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动物毛发中兽药残留检测进展 总被引：3，自引：0，他引：3
动物毛发是兽药残留检测中的新型检材,本文对动物毛发中兽药残留的相关研究作一综述。毛发主要由α-角蛋白、黑色素等物质所组成,药物吸收和累积到毛发中的主要机制包括简单的"被动扩散"和复杂的"多池模型"。毛发的颜色和药物的服用方式也影响药物积聚在毛发中的速率和可能方式。常用的毛发样品处理方式包括清洗、水解、提取和净化等步骤。已被研究的药物包括克伦特罗、激素、磺胺、喹诺酮、四环素等,已用的检测手段包括酶联免疫分析法、气质联用法和液质联用法。目前对动物毛发分析存在机制不明确、质量控制存在争议等问题,动物毛发分析仍具有发展前景。 相似文献
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动物毛发下脚料中粗蛋白质的测定 总被引：2，自引：0，他引：2
王仁华 《江西畜牧兽医杂志》2002,(4):26-26
<正>1 动物毛发下脚料中粗蛋自质的特性 毛发角蛋白是索状结构的高分子化合物,其基本单位是个氨基酸L-构型,属角质蛋白质,由碳、氢、氧、氮4种元素构成,其化学结构是多肽链沿纤维轴平行螺旋排列而成,链与链之间是二硫键和氢键联接。毛发角蛋白不能直接被动物消化利用,因此,必须通过高温、高压、酸、碱或酶等处理,使其索状结构的高分子断裂,变为低分子结构的蛋白源或游离氨基酸,动物才能消化吸收利用。 相似文献
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Fifty-one masked palm civets (Paguma larvata) were trapped as part of a nuisance wildlife control programme between July 2001 and August 2002 in Fujisawa City, Kanagawa Prefecture, Japan. Eleven civets had characteristic mange lesions with marked alopecia and crusts, caused by the burrowing epidermal mite Notoedres cati. The diagnosis was confirmed by histology and examination of mites obtained from skin scrapings of affected civets. Histopathology of lesions demonstrated moderate hyperplastic epidermis, parakeratosis and acanthosis. Occasionally microvascular angiogenesis was observed in the epidermis. Tunnels were excavated in the hair follicles, reaching the hair roots. The tunnels were located between the hair shaft and the inner root sheath. Female mites, eggs and nymphs were demonstrated in their tunnels using scanning electron microscopy. 相似文献
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Songtao GUO Min QIANG Xiaorui LUAN Pengfei XU Gang HE Xiaoyan YIN Luo XI Xuelin JIN Jianbin SHAO Xiaojiang CHEN Dingyi FANG Baoguo LI 《Integrative zoology》2015,10(6):572-578
For ecologists, understanding the reaction of animals to environmental changes is critical. Using networked sensor technology to measure wildlife and environmental parameters can provide accurate, real‐time and comprehensive data for monitoring, research and conservation of wildlife. This paper reviews: (i) conventional detection technology; (ii) concepts and applications of the Internet of Things (IoT) in animal ecology; and (iii) the advantages and disadvantages of IoT. The current theoretical limits of IoT in animal ecology are also discussed. Although IoT offers a new direction in animal ecological research, it still needs to be further explored and developed as a theoretical system and applied to the appropriate scientific frameworks for understanding animal ecology. 相似文献
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我国经济野生动物养殖及其发展对策 总被引：2，自引：2，他引：0
本文论述了我国经济野生动物养殖发展历程、目前养殖种类及研究工作进展情况 ,同时 ,指出了我国经济野生动物养殖业存在的问题 ,提出了加强养殖行业的协调及管理 ,以经济野生动物产品及加工开发促进养殖业的发展 ,以养殖促进野生动物保护事业发展的战略对策 ,使野生经济动物继续为人类利用 ,造福于人民 ,造福于社会 相似文献
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为比较2、35日龄滩羊皮肤毛囊的发育特点与血管内皮生长因子（vascular endothelial growth factor，VEGF）和血管内皮生长因子受体2（vascular endothelial growth factor receptor 2，VEGFR2）的分布特征，探究出生后滩羊被毛生长发育的变化特点，试验应用常规HE染色及改良Masson胶原纤维染色、Gomori银氨法染色、磷钨酸染色等特殊染色观察2与35日龄滩羊皮肤组织结构特点；应用免疫组织化学法结合免疫荧光染色法观察VEGF及VEGFR2在2与35日龄滩羊皮肤组织中的分布定位，并用IPP图像分析软件进行定量分析。结果显示：与2日龄滩羊皮肤组织比较，35日龄滩羊表皮与真皮间界限更加清晰，毛囊结构发育完整；毛囊密度显著降低（P<0.05）；胶原纤维与弹性纤维含量增加，形成网格状分布。免疫组化及免疫荧光结果显示，VEGF及VEGFR2在滩羊皮肤表皮及毛囊外根鞘、皮脂腺上均有表达。统计表明，VEGF及VEGFR2在2日龄滩羊皮肤组织中的表达量均显著高于35日龄（P<0.05）。综合上述结果，滩羊毛囊发育过程中，胶原纤维和弹性纤维增加明显；VEGF与VEGFR2通路在毛囊角质形成中起直接调节作用。 相似文献
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为掌握德宏州陆生野生动物资源现状及其动态变化状况,分析德宏州当前野生动物繁育及经营利用存在的问题,以提出做好野生动物繁育及经营利用的建议,达到野生动物保护与开发利用共赢的目的,德宏州野生动物收容救护中心成立调查组,协同德宏州林业局保护办及芒市、梁河县、盈江县、陇川县、瑞丽市保护办对全州野生动物繁育情况进行了详细调查。经过调查,德宏州共有野生动物养殖户102户,养殖野生动物25种119 687头(条、只),从业人数307人;总固定资产1. 18亿元,养殖场(厂)总面积151. 9 hm^2;野生动物产品加工企业3户,主要产品有熊胆干粉、熊胆胶囊和鲜鹿茸;野生动物年平均交易额3 864. 3万元。目前主要存在野生动物养殖技术落后、较少使用野生动物保护标识、资金缺乏保障等问题,本文根据存在的问题提出了发展对策。 相似文献
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Saridomichelakis MN Koutinas AF Farmaki R Leontides LS Kasabalis D 《Veterinary dermatology》2007,18(2):138-141
This study was designed to compare the sensitivity of deep skin scraping, hair plucking, and exudate microscopy for the diagnosis of canine demodicosis. Sixty-seven dogs diagnosed with demodicosis were enrolled in the study. Thirty dogs had localized and 37 had generalized demodicosis. Twenty-seven of the 67 dogs had complicated (secondarily infected) and 40 had noncomplicated form. On each dog, a single lesion was randomly selected to obtain one deep skin scraping, hair plucking, and, when present (n = 13) exudate. For skin scraping and exudate microscopy, an area under a cover slip measuring 2.2 x 2.2 mm was examined, while trichography included the evaluation of 100 hair shafts. At least one parasitic element was found in 85.1% of trichograms, and 100% of exudate preparations. The number of parasitic elements was higher in skin scrapings compared to the other two methods. The diagnostic sensitivity of skin scrapings was higher than that of hair pluckings for the total number of samples (P = 0.002) and for those obtained from dogs with the localized (P = 0.004) and the noncomplicated (P = 0.002) forms of the disease. The diagnostic sensitivity of hair pluckings was higher in generalized and complicated demodicosis compared to the localized and noncomplicated variants. Based on these results, exudate microscopy may be equally sensitive to deep skin scrapings, and trichography may be of value in generalized and complicated demodicosis, although a negative result cannot rule it out. 相似文献
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This article reports on the development and implementation of a qualitative methodology to measure wildlife value orientations (WVOs) in a focus group setting, with the ultimate goal of developing a technique to help with cross-cultural assessments of WVOs. Information provided by such assessments can assist conservation organizations in understanding and meeting the needs of an increasingly diverse population. The technique was administered to Latino and Chinese-American audiences in New York City. Focus group participants were shown a series of photographs depicting wildlife and human–wildlife interactions, and asked to individually evaluate each photograph and discuss their reactions. Results revealed this methodology to be effective in eliciting WVOs. Four WVO types recognized from previous literature were identified across the groups based on participant comments. This article concludes with suggestions on how to improve this methodology for future use and adapt it for applications in other settings. 相似文献
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水獭针毛形态结构的稳定性与变异性的系统研究 总被引：8，自引：0，他引：8
选用非明显季节性换毛的水獭皮为实验材料，通过扫描电子显微镜观察毛的微观结构并系统比较了不同个体的相同身体部位、同一个体的不同部位以及不同生长阶段的各种针毛在微观结构上的差异性与稳定性。结果表明，水獭针毛的微观结构既有种的稳定性，也有因不同部位和不同生长阶段等的结构差异．从而提出应用毛的微观结构进行兽类分类与鉴别必须把握毛的类型、毛的生长阶段及所处的身体部位的可比性。 相似文献