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1.
Resistance of early-maturing European maize germplasm to sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) 总被引：3，自引：0，他引：3
L. Kuntze E. Fuchs M. Grüntzig B. Schulz D. Klein A. E. Melchinger 《Plant Breeding》1997,116(5):499-501
Sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) are the most important viruses of maize in Europe. In field and greenhouse experiments, 122 early-maturing European maize inbreds (45 flint and 77 dent lines) were evaluated for their reaction to artificial inoculation by SCMV and MDMV. Three dent inbreds (D21, D32, FAP1360 A) with complete resistance and four dent inbreds (D06, D09, R2306, FAP1396A) with partial resistance against both potyviruses under both greenhouse and field conditions were identified. All other inbreds were highly susceptible to both SCMV and MDMV. Selection for virus resistance in maize breeding could be performed with only one virus at a time because all inbreds resistant to SCMV were also resistant to MDMV. Rank correlations between percentages of infected plants in greenhouse and field trials ranged from 0.51 to 0.72 for both SCMV and MDMV, suggesting that prescreening of breeding materials for virus resistance can be performed in the greenhouse but final evaluation in multilocation trials in the field is recommended. 相似文献
2.
Molecular mapping and gene action of Scm1 and Scm2, two major QTL contributing to SCMV resistance in maize 总被引：4，自引：0，他引：4
Sugarcane mosaic virus (SCMV) is one of the most important virus diseases of maize in Europe. In this study, the gene action at two major quantitative trait loci (QTL) affecting resistance to SCMV in maize was mapped and characterized. A total of 121 F3 lines from cross F7 (susceptible) × FAP1360A (resistant) were evaluated for SCMV resistance in replicated field trials across two environments under artificial inoculation at seven scoring dates. The genotypic variance was always highly significant and heritability increased up to 0.92 for later scoring dates. The method of composite interval mapping was employed for QTL mapping using four simple sequence repeat (SSR) markers flanking two regions identified in a previous study with cross D145 × D32. The presence of two QTL for SCMV resistance, one on chromosome 6 (Scml region) and one on chromosome 2 (Scm2 region), was confirmed. These two QTL together explained between 15% (first score) and 62% (final score) of the phenotypic variance at various stages of plant development. Gene action was additive for the Scm1 region but completely dominant for the Scm2 region. Comparison of results of this study with those obtained for cross D145 × D32 suggested that the resistance alleles in the two populations are identical for the Scm1 region but different for the Scm2 region. 相似文献
3.
4.
Targeted BSA mapping of Scmv1 and Scmv2 conferring resistance to SCMV using PstI/MseI compared with EcoRI/MseI AFLP markers 总被引：2，自引：0，他引：2
L. Yuan C. M. Duble J. Muminovic A. E. Melchinger T. Lübberstedt 《Plant Breeding》2004,123(5):434-437
In a previous study, two chromosome regions (Scmv1 and Scmv2), conferring sugarcane mosaic virus (SCMV) resistance in maize, were enriched with EcoRI/MseI AFLP (Eco‐AFLP) markers (methylation insensitive) by targeted bulked segregant analysis (tBSA). The objective of the present study was to further saturate these two regions with PstI/MseI AFLP (Pst‐AFLP) markers (methylation sensitive) using the same tBSA approach, and to compare the genomic distribution of both Pst‐AFLP and Eco‐AFLP markers. Out of 470 PstI/MseI primer combinations screened, four Pst‐AFLP markers were identified in the Scmv1 region (chromosome 6), and none in the Scmv2 region (chromosome 3). These Pst‐AFLP markers were more closely linked to the Scmv1a gene than any of the Eco‐AFLP markers, and could be useful for marker‐assisted selection and even map‐based cloning. In addition, Pst‐AFLP and Eco‐AFLP markers were dissimilarly distributed in both target regions. Pst‐AFLP markers were equally distributed across both regions, while Eco‐AFLPs were clustered in the Scmv2 region. 相似文献
5.
一个新的抗玉米矮花叶病基因的发现及初步定位 总被引：3，自引：0，他引：3
由SCMV引起的矮花叶病是我国的主要玉米病害之一, 鉴定和发掘新的抗病基因对于玉米抗病遗传育种具有重要意义。以抗病自交系海9-21和感病自交系掖478杂交的一个BC2F3群体为试验材料, 通过人工接种矮花叶病毒进行抗病性鉴定, 发现该分离群体中抗病植株与感病植株数符合1∶3的分离比例, 推测其抗病基因是由1对隐性基因控制。抗感池和SSR标记连锁分析表明, 存在一个新的玉米矮花叶病隐性抗病基因(或等位基因), 将该基因命名为scm3。scm3基因来源于抗病玉米自交系海9-21, 位于第3染色体短臂3.04~3.05区域, 在SSR标记umc1965和bnlg420之间, 遗传距离分别为45.7 cM和6.5 cM。连锁的标记还有umc1307、umc2265、bnlg2241和umc2166, 它们与scm3之间的遗传距离分别是8.3、13.3、15.5和19.7 cM, 这些SSR标记与scm3基因在染色体上的排列顺序为umc1965—scm3—bnlg420—umc1307—umc2265—bnlg2241—umc2166。 相似文献
6.
A melon (Cucumis melo L.) breeding line derived from PI 414723 is resistant to three potyviruses,watermelon mosaic virus (WMV), zucchini yellow
mosaic virus (ZYMV), papaya ringspot virus (PRSV), and to powdery mildew (PM). The inheritance and linkage relationships of
these four resistances were studied in a segregating F2 population and derived F3 families from a cross between cultivar Top
Mark and the resistant breeding line. Dominant monogenic inheritance of all four resistances was observed. We report that
line 414723-4S3, which was initially selected as a source of ZYMV and WMV resistance, is also a source of dominant monogenic
resistances to PRSV and PM race 1. We also report on genetic linkage (significant departure from independent segregation,
χ2 = 58.1, p≪ 0.0001) between resistance to WMV and ZYMV. The map distance between these loci was estimated to be 7.5 cm. The genes for
resistance to PM and PRSV segregated independently from each other, and from ZYMV and WMV resistance.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
7.
Role of male-sterile cytoplasm in resistance to barley yellow mosaic virus and Fusarium head blight in barley 总被引：2，自引：0，他引：2
Two types of male‐sterile cytoplasm, designated msm1 and msm2, in barley were investigated to determine whether these cytoplasms confer resistance to barley yellow mosaic virus (Ba YMV) and Fusarium head blight (FHB). Alloplasmic lines and isogenic lines of two cultivars showed the same reaction to each Ba YMV as that of their euplasmic lines. This demonstrates that the barley male‐sterile cytoplasms msm1 and msm2 have no effect on resistance to BaYMV. No significant difference in reactions to FHB was recognized among fertile alloplasmic lines of ‘Adorra’, but the difference in reactions to FHB between fertile and sterile isogenic lines of ‘Adorra’ was significant. The damage caused by FHB in the male‐sterile lines that produced sterile pollen was significantly greater than the damage in a sterile line that did not produce pollen. These results suggest that pollen or anthers are important factors in infection with or spread of FHB. For production of hybrid seeds, male‐sterile lines with no pollen production, such as those with msm1 male‐sterile cytoplasm, would reduce FHB infestation. 相似文献
8.
9.
Sugarcane mosaic virus (SCMV) is one of devastating pathogens in maize (Zea mays L.), and causes serious yield loss in susceptible cultivars. An effective solution to control the virus is utilizing resistant
genes to improve the resistance of susceptible materials, whereas the basic work is to analyze the genetic basis of resistance.
In this study, maize inbred lines Huangzao4 (resistant) and Mo17 (susceptible) were used to establish an F9 immortal recombinant inbred line (RIL) population containing 239 RILs. Based on this segregation population, a genetic map
was constructed with 100 simple sequence repeat (SSR) markers selected from 370 markers, and it covers 1421.5 cM of genetic
distance on ten chromosomes, with an average interval length of 14.2 cM. Analysis of the genetic map and resistance by mapping
software indicated that a major quantitative trait locus (QTL) was between bin6.00 and bin6.01 on chromosome 6, linked with
marker Bnlg1600 (0.1 cM of interval). This QTL could account for 50.0% of phenotypic variation, and could decrease 27.9% of
disease index. 相似文献
10.
Summary The mode of inheritance for resistance to zucchini yellow mosaic virus (ZYMV) in Cucurbita moschata was determined from F1, F2, and backcross progenies of the cross between the susceptible cultivar Waltham Butternut from the U.S.A. and a resistant inbred line of the Menina cultivar from Portugal. Resistance to ZYMV in Menina was conferred by a single dominant gene designated Zym. 相似文献
11.
复合侵染甘蔗的病原病毒RT-PCR检测 总被引：1，自引：1，他引：0
根据甘蔗花叶病毒（sugarcane mosaic virus,SCMV）、高粱花叶病毒（sorghum mosaic virus,SrMV）和甘蔗黄叶病毒（sugarcane yellow leaf virus,SCYLV）外壳蛋白（CP）基因序列分别设计合成3对特异引物SCMV–F/R、SrMV-F/R和SCYLV-F/R,以表现花叶和黄叶复合症状的甘蔗叶片总RNA为模板,分别进行3种病毒单一RT-PCR检测,在SrMV和SCYLV特异引物RT-PCR反应体系中分别扩增到约850 bp和630 bp特异性片段。序列测定及同源性比对结果显示,850 bp片段测序所得序列与浙江象山、临平和余杭SrMV分离物（GenBank登录号分别为AJ310194、AJ310195和AJ310198）对应序列同源性95%,630 bp片段测序所得序列与广东、广西、福建SCYLV分离物（GenBank登录号分别为GU190165、GU190162、GU190159）对应序列同源性99%,表明该样品同时感染SrMV和SCYLV。在此基础上建立同时检测SrMV和SCYLV的一步双重RT-PCR体系,可检测10-6 g病叶组织中的病毒,田间样品检测效果良好。 相似文献
12.
Francis Kusi Francis K. Padi Daniel Obeng‐Ofori Stephen K. Asante Richard Y. Agyare Issah Sugri Michael P. Timko Robert Koebner Bao‐Lam Huynh Jansen R. P. Santos Timothy J. Close Philip A. Roberts 《Plant Breeding》2018,137(2):203-209
The utility of combining simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) marker genotyping was determined for genetically mapping a novel aphid (Aphis craccivora) resistance locus in cowpea breeding line SARC 1‐57‐2 and for introgressing the resistance into elite cultivars by marker‐assisted backcrossing (MABC). The locus was tagged with codominant SSR marker CP 171F/172R with a recombination fraction of 5.91% in an F2 population from ‘Apagbaala’ x SARC 1‐57‐2. A SNP‐genotyped biparental recombinant inbred line population was genotyped for CP 171F/172R, which was mapped to position 11.5 cM on linkage group (LG) 10 (physical position 30.514 Mb on chromosome Vu10). Using CP 171F/172R for foreground selection and a KASP‐SNP‐based marker panel for background selection in MABC, the resistance from SARC 1‐57‐2 was introduced into elite susceptible cultivar ‘Zaayura’. Five BC4F3 lines of improved ‘Zaayura’ that were isogenic except for the resistance locus region had phenotypes similar to SARC 1‐57‐2. This study identified a novel aphid resistance locus and demonstrated the effectiveness of integrating SSR and SNP markers for trait mapping and marker‐assisted breeding. 相似文献
13.
Barley yellow mosaic disease is caused by several viruses, i.e. barley yellow mosaic virus (BaYMV), barley mild mosaic virus (BaMMV) and BaYMV-2. The reaction of different barley germplasms to the barley mosaic viruses was studied in field and greenhouse experiments. The results show a complex situation; some varieties are resistant to all the viruses, while others are resistant to one or two of them only. Crosses between different barley germplasms were earned out in order to test whether genetic diversity of resistance against mosaic viruses does exist, particularly, BaMMV. A total of 45 foreign barley varieties were crossed to German cultivars carrying the resistance gene ym4. In F2 of 27 crosses, no segregation could be detected, leading to the conclusion that the resistance genes of the foreign parents are allelic with ym4 e.g. Ym1 (‘Mokusekko 3’) and Ym2 (‘Mihori Hadaka 3’). A total of 18 crosses segregated in F2 indicating that foreign parents, like ‘Chikurin Ibaraki 1’, ‘Iwate Omugi 1’, and “Anson Barley”, carry resistance genes different from the gene of German cultivars, e.g. ‘Asorbia’ or ‘Franka’. By means of statistical evaluation (Chi2-test), the observed segregation ratios were analyzed in order to obtain significant information on the heredity of resistance. All the resistance genes described here as being different from the gene ym4, act recessively. Most of the exotic varieties seem to carry only one resistance gene. In a few cases, more than one gene may be present. 相似文献
14.
Josias C. Faria Paula A. M. R. Valdisser Elsa O. P. L. Nogueira Francisco J. L. Aragão 《Plant Breeding》2014,133(5):649-653
Bean golden mosaic virus (BGMV) is the causal agent of bean golden mosaic of common beans. A transgenic bean line that has been developed based on RNA interference to silence the BGMV rep gene showed immunity to the virus. Crosses were done between the transgenic line and six bean cultivars followed by four backcrosses to the commercial cultivars ‘Pérola’ and ‘BRS Pontal’. The transgene locus was consistently inherited from the crosses analysed in a Mendelian fashion in the segregating populations. The disease resistance reaction co‐inherited with the transgene. Nevertheless, the expression of disease resistance displayed a dosage effect phenomenon in the F1 generation. The analysis of the homozygous near‐isogenic lines in field conditions, under high BGMV disease incidence, indicated that the transgenic lines were completely resistant. These results show the strength of the disease resistance obtained, the stability of the trait across generations and its usefulness in the management of a disease for which there is no reported Phaseolus germplasm with immunity. 相似文献
15.
反义外壳蛋白基因介导的抗SCMV转基因玉米研究 总被引：6，自引：1，他引：6
玉米矮花叶病（MDM）是一种世界性病害，在我国主要由甘蔗花叶病毒(SCMV)所致。为探索一条高效、安全的抗SCMV转基因途径，构建了无标记基因的SCMV反义外壳蛋白基因（cp）表达载体pACP。通过冻融法将该载体与抗除草剂标记基因(bar)载体分别导入农杆菌LBA4404,然后共转化玉米自交系综3的幼胚。通过除草剂梯度筛选，从抗性愈伤组织分化获得了35株再生苗。PCR检测证明，其中26株带有抗除草剂标记基因（bar），14株带有SCMV反义cp基因。这14株带有目的基因的玉米植株自交，其种子在田间种植成株行（T1代）。玉米T1代幼苗人工接种SCMV，筛选出2个抗病株率高于70%的株行。ELISA检测表明，抗病株SCMV含量极低，抗性达高抗水平。PCR检测表明，抗病性是反义cp基因作用的结果，并且获得了2株cp基因阳性而标记基因阴性的抗病株。 相似文献
16.
Genetic analysis and identification of two soybean mosaic virus resistance genes in soybean [Glycine max (L.) Merr] 
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下载免费PDF全文 Kai Li Rui Ren Karthikeyan Adhimoolam Le Gao Yuan Yuan Zhitao Liu Yongkun Zhong Haijian Zhi 《Plant Breeding》2015,134(6):684-695
Soybean mosaic virus (SMV) commonly affects soybean production worldwide, and the SC18 strain has been widespread in China. This study aimed to characterize and map the SC18 resistance genes present in soybean cultivars ‘Kefeng No. 1’ and ‘Qihuang 22’. Inheritance analysis revealed that two independent single dominant genes in Kefeng No. 1 and Qihuang 22 confer resistance to SC18. Using simple sequence repeat (SSR) markers and bulked segregant analysis, the Kefeng No. 1 and Qihuang 22 resistance genes were located on soybean chromosomes 2 and 13, respectively. We further screened two populations of recombinant inbred lines with 32 SSR markers in the target region, where the resistance gene in Kefeng No. 1 was fine mapped to an 80‐kb region containing six putative genes. Sequence and expression analyses of these genes revealed that SMV resistance in Kefeng No. 1 was probably attributable to three of the candidate genes (i.e. Glyma.02G127800, Glyma.02G128200 and Glyma.02G128300). Collectively, the results of this study will greatly facilitate the cloning of SC18 resistance genes and marker‐assisted breeding of SMV‐resistant soybean cultivars. 相似文献
17.
Ratanakorn Kitsanachandee Prakit Somta Orawan Chatchawankanphanich Khalid P. Akhtar Tariq Mahmud Shah Ramakrishnan M. Nair Tejinderjit S. Bains Asmita Sirari Livinder Kaur Peerasak Srinives 《Breeding Science》2013,63(4):367-373
Yellow mosaic disease (YMD) is one of the major diseases affecting mungbean (Vigna radiata (L.) Wilczek). In this study, we report the mapping of the quantitative trait locus (QTL) for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean. An F8 recombinant inbred line (RIL) mapping population was generated in Thailand from a cross between NM10-12-1 (MYMIV resistance) and KPS2 (MYMIV susceptible). One hundred and twenty-two RILs and their parents were evaluated for MYMIV resistance in infested fields in India and Pakistan. A genetic linkage map was developed for the RIL population using simple sequence repeat (SSR) markers. Composite interval mapping identified five QTLs for MYMIV resistance: three QTLs for India (qYMIV1, qYMIV2 and qYMIV3) and two QTLs for Pakistan (qYMIV4 and qYMIV5). qYMIV1, qYMIV2, qYMIV3, qYMIV4 and qYMIV5 explained 9.33%, 10.61%, 12.55%, 21.93% and 6.24% of variation in disease responses, respectively. qYMIV1 and qYMIV4 appeared to be the same locus and were common to a major QTL for MYMIV resistance in India identified previously using a different resistant mungbean. 相似文献
18.
Identification and validation of a quantitative trait locus associated with wheat yellow mosaic virus pathotype I resistance in a Japanese wheat variety 下载免费PDF全文
下载免费PDF全文 Hisayo Kojima Zenta Nishio Fuminori Kobayashi Mika Saito Takahide Sasaya Chikako Kiribuchi‐Otobe Masako Seki Shunsuke Oda Toshiki Nakamura 《Plant Breeding》2015,134(4):373-378
Yellow mosaic disease, caused by wheat yellow mosaic virus (WYMV), is one of the most serious diseases of winter wheat (Triticum aestivum L.) in Japan. The three pathotypes of WYMV are distributed in different geographical areas: pathotype I is found mainly in western and central Japan (Kanto), pathotype II in northern Japan (Tohoku and Hokkaido) and pathotype III on the southern island of Japan (Kyushu). A total of 246 doubled‐haploid (DH) lines, derived from a cross between ‘Yumechikara’ (resistant) and ‘Kitahonami’ (susceptible), were evaluated for 2 years for their resistance to WYMV pathotype I. A single major quantitative trait locus, Q.Ymym, mapping to chromosome 2D was associated with resistance to pathotype I in ‘Yumechikara’. This is the first time a QTL responsible for pathotype I resistance has been identified. Fine mapping of Q.Ymym indicated that it was on a tight linkage block originating from ‘Yumechikara’, and the markers associated with this block will accelerate the development of varieties resistant to WYMV pathotype I. 相似文献
19.
大豆花叶病引起的大豆顶端坏死症 总被引：5，自引：0，他引：5
２个抗病亲本和２个感病亲本配制４个杂交组合和４个回交组合。调查其Ｆ１、Ｆ２和ＢＣＦ１群体接种东北大豆花叶病毒二号株系后，顶端坏死株的形成和分离比例。Ｆ１表现两种类型：无症株和坏死株，Ｆ２表现三种类型：无症株、有症株（花叶、皱缩等）和坏死株。其分离比例或为３抗：１感，或为７抗；９感，χ＾２测验符合一对显性基因控制或者两对隐性互补基因控制。 相似文献