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1.
This study was designed to determine the effect of complete substitution of fish meal (FM) by three plant protein sources including extruded soybean meal (SBM), extruded full‐fat soybean (FFSB) and corn gluten meal (CGM) on growth and feed utilization of Nile tilapia Oreochromis niloticus and tilapia galilae Sarothrodon galilaeus. Four isonitrogenous of crude protein (ca. 28.0%) and isocaloric (ca. 19 MJ kg−1) experimental diets were formulated. The control diet (diet 1) was prepared with FM as the main protein sources. Diets 2–4, each FM control diet, were completely substituted with SBM (diet 2), FFSB (diet 3) and CGM (diet 4). l ‐lysine and dl ‐methionine were added to plant protein diets to cover the nutritional requirements of tilapia. Each treatment was allocated to three net pens and fed for 17 weeks. Nile tilapia fed the control diet showed significantly higher (P≤0.05) values for final body weight (FBW), feed intake (FI), weight gain (WG) and specific growth rate (SGR), whereas fish fed the diet with CGM achieved the lowest values. Tilapia galilae fed SBM diet recorded the highest (P≤0.05) values for growth performance. Better feed conversion ratio (FCR) for both Oreochromis niloticus and Sarothrodon galilaeus was observed when fish were fed SBM diet, whereas the worse FCR was recorded for FFSB diet. Feed utilization parameters including protein productive value (PPV), fat retention (FR) and energy retention (ER) showed significant differences (P≤0.05) for both the species fed different dietary protein sources. The present results suggest that, for Nile tilapia, both SBM and FFSB supplemented with dl ‐methionine and l ‐lysine can completely replace dietary FM. Meanwhile, S. galilaeus fed SBM diet exhibited comparable growth and feed utilization with those fish fed a fish‐meal‐based diet.  相似文献   

2.
A 8‐week feeding trial was conducted to determine the effect of substituting fish oil with palm oil‐laden spent bleaching clay (SBC), a by‐product from crude palm oil (CPO) refining, on growth, feed utilization, fatty acid composition and heavy metal accumulation in the muscle of Nile tilapia, Oreochromis niloticus. Four isonitrogenous and isolipidic practical diets were formulated to contain 0, 100, 200 or 300 g kg?1 SBC. Growth performance of Nile tilapia was significantly better in fish fed the 100 g kg?1 SBC diet compared with fish fed the 0, 200 or 300 g kg?1 SBC diet. Growth and feed utilization efficiency of fish fed 200 or 300 g kg?1 SBC were similar to fish fed the control diet without added SBC. Whole‐body composition, body‐organ indices and haematocrit of tilapia were not affected by dietary treatments. Fatty acid compositions in the muscle lipid of Nile tilapia were strongly influenced by dietary treatments with progressively elevated levels of total saturates and n‐6 PUFA because of the dietary influence of these fatty acids from residual CPO adsorbed onto SBC. A gradual decrease in total n‐3 PUFA concentrations were also observed with the ratio of n‐3 to n‐6 fatty acids in muscle lipids decreasing from 4.75 to 4.41, 3.23 or 2.37 after 8 weeks on the 0, 100, 200 or 300 g kg?1 SBC diet, respectively. The arsenic, cadmium and lead concentrations in the experimental diets increased with increasing dietary levels of SBC but the concentrations of these heavy metals in the whole body and bone of Nile tilapia were not significantly different among fish fed the various diets. The present 8‐week study showed that in fishmeal‐based diets for Nile tilapia, palm oil‐laden SBC can totally replace added fish oil. The use of this presently discarded waste product from palm oil refining in tilapia diets will greatly contribute to reducing the impact of rising feed costs in the culture of tilapia in many tropical countries. Other potential benefits may include acting as a feed binder, removal of mycotoxins in fish feeds as well as adsorbing toxic substances present in the culture water.  相似文献   

3.
Aquaculture of yellow perch (Perca flavescens) has been increasing, yet there have been few nutritional studies and no evaluations of alternative protein sources in diets. Solvent‐extracted, dehulled soybean meal (SBM) and expelled‐extruded soybean meal (exSBM) were fed to yellow perch to evaluate their effectiveness in replacing dietary fish meal (FM) in isonitrogenous practical feed formulations. Both soy ingredients were incorporated in graded amounts from 100 to 730 g kg−1 of the diet. Feed consumption, weight gain, feed efficiency (FE) and survival were significantly affected by type of soy ingredient, concentration and the interaction of the two main effects. Consumption was significantly lower in fish fed diets containing 400, 600 and 730 g kg−1 compared to fish fed diets containing lower concentrations. Weight gain was significantly lower in fish fed diets containing 600 g kg−1 and FE significantly lower in fish fed diets containing 500 g kg−1 compared to fish fed the control diet or lower concentrations of soy ingredients. Most fatty acid concentrations were affected by feeding exSBM compared to fish fed the control diet, but long chain fatty acids remained at relatively high concentrations. Based on feed consumption, weight gain and FE data, yellow perch are able to effectively utilize both soy ingredients in practical diets. A conservative recommendation of 300 g kg−1 diet appears appropriate for growout diets.  相似文献   

4.
Replacement of fish meal (FM) protein with dehulled and solvent‐extracted plant by‐products, soybean meal, cottonseed meal, sunflower meal, and linseed meal was tested in diets for juvenile Nile tilapia, Oreochromis niloticus. Fish averaging (±SD) 14.2 ± 2.9 g were divided into 18 groups and fed for 6 mo on pelleted feed containing each of the plant protein meal supplemented with Yucca schidigera powder extract at 750 mg/kg. Methionine (1%) and lysine (0.5%) were added to each diet except the control diet (FMC), while diet FMC + Y was supplemented with yucca only. Three groups of fish were fed each of six isonitrogenous (25% crude protein) and isocaloric (4.3 kcal/g) diets replacing 100% of FM protein and performance compared against a nutritionally balanced control and a commercial tilapia feed. After 6 mo of feeding, the fish fed plant protein diets supplemented with yucca exhibited growth performance not differing significantly from that of fish fed FMC + Y, while differing significantly from the control FMC and diet linseed meal (LSM). The highest apparent protein digestibility coefficient was observed for diets treated with yucca, which was significantly higher than that observed for the control diet FMC. No significant differences were found in whole‐body moisture of fish fed different experimental diets. An increase in the whole‐body protein content was observed in fish fed diets supplemented with yucca, which was significantly different from that of the diet FMC. The whole‐body fat content of Nile tilapia was low and showed significant differences among treatments supplemented with yucca compared with the control diet FMC. The whole‐body energy content showed the same trend as whole‐body fat content.  相似文献   

5.
Four practical diets containing 2% of crude protein (CP) (180 and 280 g kg?1), with or without menhaden fish meal (FM), were fed to Australian red claw Cherax quadricarinatus during a 97‐day feeding trial. Growth, survival, body composition and processing traits of pond‐cultured red claw were determined. Juvenile red claw (mean individual weight of 5.75 ± 3.3 g) were randomly stocked into twelve 0.04‐ha ponds at a rate of 1000 per pond (25 000 ha?1), and each diet was fed to three ponds. At harvest, the final mean weight of red claw fed Diet 4 (0 g kg?1 FM and 280 g kg?1 CP) and Diet 3 (113 g kg?1 FM and 280 g kg?1 CP) was significantly (P < 0.05) higher (62.4 and 58.5 g, respectively) than red claw fed Diet 1 (73 g kg?1 FM and 180 g kg?1 CP; 51.7 g) and Diet 2 (0 g kg?1 FM and 180 g kg?1 CP; 53.0 g). Red claw fed diets containing 280 g kg?1 CP, with or without FM, had significantly higher percent weight gain (894 and 959%, respectively) compared to red claw fed 180 g kg?1 CP, with or without FM (778 and 799%, respectively). Feed conversion ratio, percent survival, and total yield among treatments, which averaged 3.55, 65.2%, and 724 kg ha?1 overall, were not significantly different. Results from this study indicate that pond‐cultured red claw stocked at 25 000 ha?1 can be fed a practical diet containing 280 g kg?1 CP with 0 g kg?1 FM if a combination of plant‐protein ingredients (soybean meal, distillers’ dried grains with solubles, and milo) is added; however, if the percentage of dietary protein level is 180 g kg?1, growth is reduced even if FM is added at 73 g kg?1 of the diet. Use of diets containing plant‐protein sources may help reduce diet costs to producers and thereby, increase profits.  相似文献   

6.
A growth trial was conducted to examine the effect of dietary digestible energy (DE) content on methionine (Met) utilization and requirement in juvenile Nile tilapia (Oreochromis niloticus). Ten iso‐nitrogenous (288 g kg?1 protein) practical diets, with two DE levels (10.9 MJ kg?1; 12.4 MJ kg?1) and five methionine supplementation levels (0, 1, 2, 4 and 6 g kg?1), were hand‐fed twice daily to triplicate groups of Nile tilapia (initial body weight 8.95 ± 0.06 g) for 8 weeks. Weight gain (WG) and specific growth rate (SGR) increased significantly with increasing dietary methionine concentration at the same DE content (< 0.001). At the same dietary methionine level, WG and SGR of fish fed high‐DE diets were significantly higher than that of fish fed low‐DE diets (= 0.0001), although no interaction was found between dietary DE and methionine supplementation. Based on quadratic regression analysis between dietary methionine concentration and weight gain, optimal methionine requirement for maximum growth, expressed as g Met required kg?1 diet (low‐ versus high‐DE diets), increased as diet DE concentration increased (7.34 versus 9.90 g kg?1 diet, respectively; with cysteine 4.70 g kg?1 diet). The results indicated that diet DE content affects methionine utilization and requirement in juvenile Nile tilapia, fish fed high‐DE diets required more methionine for maximum growth.  相似文献   

7.
An 8‐week growth trial was conducted to determine the effects of complete replacement of fishmeal protein by soy protein concentrate (SPC) on growth performance of Nile tilapia (Oreochromis niloticus GIFT strain) fry (initial body weight 1.6 ± 0.0 g). In control diet, 135 g kg‐1 fishmeal was used, and in the other two diets, 100% of fishmeal was replaced by SPC supplemented with or without methionine hydroxy analogue (MHA) according to the content in FM diet. Fish of FM group were fed twice daily. Fish of SPC6 group were fed SPC diet six times daily. Fish of SPCM group were fed twice (SPCM2) or six times (SPCM6) daily. The results showed that complete replacement of fishmeal with SPC did not affect survival, condition factor, visceralsomatic index of Nile tilapia. Feed intake (FI) and feed conversion ratio (FCR) of fish in SPCM2 and SPC6 groups were higher than those in FM and SPCM6 groups. Specific growth rate (SGR) of fish in SPCM6 group was highest among four treatments. Productive protein value (PPV) of SPCM2 and SPC6 groups were significantly lower than that of FM group. Fishmeal could be completely replaced by SPC without negative effect on growth by MHA supplementation and increasing feeding frequency.  相似文献   

8.
This study was to assess effects of the pretreatment in all‐plant based diets with microbial phytase on phosphorous utilization and growth performance of Nile tilapia (Oreochromis niloticus). Pretreatment trials were conducted using phytase at graded doses to determine the optimal dose of phytase. Available phosphorus (P) levels increased significantly with the increased doses of phytase and the dose of 1000 U kg?1 was most efficient. Based on the pretreatment trials, plant based diets for Nile tilapia were formulated by pretreating with phytase at 1000 U kg?1. Experimental diets were supplemented with graded levels of mono calcium phosphate (MCP) at 25, 18.75, 12.5, 6.25 and 0 g kg ?1 diet. In addition, there were three controls: one phytase control, one inorganic P control and one pretreatment control. The results showed that diets pretreated with phytase gave better growth performance, feed conversion ratio and protein efficiency ratio of Nile tilapia compared with the phytase control diet and pretreatment control diet (P < 0.05). There were no significant differences in growth performance of Nile tilapia between the inorganic control diet and phytase pretreated diets supplemented with MCP at 25, 18.75 and 12.5 g kg?1 (P > 0.05), which resulted in significantly better performance than those at 6.25 and 0 g kg?1 (P < 0.05). Dietary interaction effects of phytase were observed for phosphorus retention efficiency and phosphorus load. Apparent digestibility coefficient of P (ADCp) was improved significantly by phytase pretreatment (P < 0.05). No significant difference was detected on ADC of crude protein among all experimental diets (P > 0.05).  相似文献   

9.
An 8‐week growth trial was conducted using a 2 × 3 factorial design to evaluate the effect of substitution of fishmeal (FM) by rendered animal protein blend [APB, comprised of 400 g kg?1 poultry by‐product meal, 350 g kg?1 meat and bone meal, 200 g kg?1 hydrolysed feather meal (HFM) and 50 g kg?1 spray‐dried blood meal] in diets of Siberian sturgeon, Acipenser baerii Brandt. Two isoenergetic control diets were formulated to contain two different protein levels [high‐protein control (400 g kg?1), with 483 g kg?1 of FM] and [low‐protein control (360 g kg?1), with 400 g kg?1 of FM]. At each protein level, dietary FM protein was replaced by APB at 75% and 100% levels and supplemented with crystallized essential amino acid under ideal protein concept. The six diets were named as HC, HAPB75, HAPB100, LC, LAPB75 and LAPB100, respectively. No significant differences were found in weight gain rate (WGR) and specific growth rate (SGR), but fish fed with the low‐protein diets showed higher feed intake and feed conversion ratio. Plasma growth hormone and insulin‐like growth factors I of each group were not significantly different (P > 0.05). The whole‐body composition and liver composition were not affected by dietary protein levels, replacement or their interaction. Muscle protein and lipid contents of fish fed with diet LAPB100 were significantly lower than those of HC group. Digestibility of nitrogen (N) and phosphorus (P) were reduced with higher APB inclusion levels, but productive N and P values of all groups were not different. Lower N and P intake induced lower nutrients losses (P < 0.05). The results suggested that dietary protein level could be reduced to 360 g kg?1 from 400 g kg?1 without affecting WGR or SGR and significantly reduced nutrients lose. Furthermore, dietary FM protein can be totally replaced by APB in feed formulation either at 400 g kg?1 or at 360 g kg?1 protein level.  相似文献   

10.
This study was undertaken in a closed system with Nile tilapia (Oreochromis niloticus) to examine the effects of total replacement of fish meal (FM) by soybean meal. Nile tilapia fingerlings with an average weight of 5.34±0.08 g were hand‐fed one of the five isoenergetic (≈13.5 MJ digestible energy kg?1) and isoproteic (≈31% of digestible protein) experimental diets to satiation, six times a day during 85 days in eight replicate fibreglass tanks (six fish per tank). The control diet containing FM was substituted by soybean meal, with and without essential amino acids (lysine, methionine and threonine) or dicalcium phosphate supplementation. The supplemental amino acids were added at levels to simulate the reference amino acid profile of Nile tilapia carcass protein, based on the ideal protein concept. The results showed that soybean meal diet supplemented only with dicalcium phosphate was inferior to the control diet with FM and soybean meal diets supplemented with dicalcium phosphate and essential amino acids. Multiple essential amino acids and dicalcium phosphate incorporation in soybean meal diets was associated with performance, whole‐body composition and carcass yield equal to that of the fish fed with the control diet containing FM. These data suggest that a diet with all plant protein source, supplemented with essential amino acids, based on tissue amino acid profile, can totally replace FM in a diet for Nile tilapia, without adverse effects on the growth performance, carcass yield and composition.  相似文献   

11.
Economical, nutritious diets for hybrid striped bass (HSTB) are required for the continued expansion and sustainability of this industry. Turkey meal (TM) is a by‐product of the US turkey industry and is a potentially‐valuable local, alternative protein source for use in aquaculture diets because of its excellent nutritional composition and quality. TM may substitute for more expensive fish meal (FM)‐based diets; however, there are no published data with regard to using this ingredient in sunshine bass diets. Therefore, a 16‐week feeding trial was conducted with juvenile (36 g) sunshine bass (Morone chrysops × Morone saxatilis) to evaluate growth, feed conversion and body composition when fed diets with decreasing levels of FM (300, 200, 100 and 0 g kg?1) and increasing levels of turkey meal (0, 97, 175 and 264 g kg?1). Four practical diets were formulated to contain 400 g kg?1 protein and similar energy levels. Twenty fish were stocked into each of the 12, 1200‐L circular tanks and were fed twice daily ad libitum. At the conclusion of the feeding trial, there were no significant (P > 0.05) differences in final mean weight, percentage weight gain, specific growth rate and feed conversion ratio among treatments, which averaged 363.7 g, 904.3%, 2.02% day?1 and 1.73, respectively. Percentage survival of fish fed diet 4 (0 g kg?1 FM and 264 g kg?1 TM) was significantly (P > 0.05) lower (survival = 88.3%) than fish fed diet 3 (100 g kg?1 FM and 175 g kg?1 TM; survival = 95%), but not different from fish fed diet 1 (survival = 92.5%) and fish fed diet 2 (survival = 93.3%). Fillet weight and amount of abdominal fat were not significantly different among all treatments and averaged 258 and 58 g kg?1, respectively. Fish fed diet 1 (300 g kg?1 FM, 0 g kg?1 TM) and diet 2 (200 g kg?1 FM and 970 g kg?1 TM) had a significantly (P < 0.05) lower hepatosomatic index (2.83 and 3.01, respectively) than fish fed diet 4 (3.33), but not different (P > 0.05) compared to fish fed diet 3 (3.14). Lipid in the fillet of fish fed diet 2 (197 g kg?1) was significantly (P < 0.05) higher than fish fed all other diets; and the percentage lipid in the fillet of fish fed diet 1 (126 g kg?1) was significantly lower than fish fed diets 2 and 4, but not different (P >0.05) compared to fish fed diet 3. Fillet moisture, protein and ash were similar among fish fed all diets and averaged 748, 798 g kg?1 and 51.0 g kg?1 (dry‐matter basis), respectively. The amino acid composition of fillets was similar among all treatments with a few slight significant differences. Results from the present study indicate that tank‐grown sunshine bass can be fed a diet containing 264 g kg?1 TM with 0 g kg?1 FM, compared to diets containing up to 300 g kg?1 FM, without adverse effects on weight gain, growth rate, feed conversion and body composition. Further research should be conducted using lower‐protein diets to determine minimum protein level for tank‐grown sunshine bass.  相似文献   

12.
Juvenile cobias, Rachycentron canadum, were fed extruded diets containing toasted defatted soybean meal (SBM) or untoasted defatted SBM [white flakes (WF)] to study growth and feed conversion, and to study if SBM induces morphological changes in the gastrointestinal (GI) tract. Three diets were produced: a fish meal‐based control diet (FM diet) with 558 g FM kg?1, and two diets with 335 g FM and either 285 g SBM kg?1 (SBM diet) or 285 g WF kg?1 (WF diet). The diets were extruded at approximately 120°C with 280 g kg?1 moisture. Triplicate groups of cobias (mean weight: 25.9 g) were fed the diets during 6 weeks. Feed intake of the FM and SBM diets were not significantly different, whereas the cumulative feed intake of cobias fed the WF diet was lower (P < 0.05) than that of cobias fed the FM and SBM diets after the first 21‐day period. Specific growth rate and feed conversion ratio were not significantly different between cobias fed the FM and SBM diets, but significantly poorer results were obtained in cobias fed the WF diet. No morphological differences in the GI tract could be attributed to the diets, and cobias fed soy did not develop enteritis in the distal intestine.  相似文献   

13.
An 8‐week feeding trial was conducted to evaluate the effects of dietary arginine on growth performance, feed utilization, haematological parameters and non‐specific immune responses of juvenile Nile tilapia (6.03 g). Five isonitrogenous and isoenergetic diets were formulated to contain graded levels of L‐arginine (8.5 g kg?1, 11.5 kg?1, 15.3 kg?1, 18.8 kg?1 and 22.4 kg?1 dry diet) from dietary ingredients and crystalline arginine. Each diet was randomly assigned to triplicate groups of 25 juvenile fish (6.04 ± 0.02 g) three times daily (8:30, 12:30, and 17:00 hours) to apparent satiation. Results showed that the weight gain (WG) and special growth rate (SGR) increased with increasing dietary arginine levels up to 15.3 kg?1 and remained nearly the same thereafter. Arginine supplementation had no impact on the survival, body composition and haematological parameters of tilapia. However, the immune responses (plasma nitric oxide content, total nitric oxide synthase (T‐NOS) and lysozyme activity) significantly (< 0.05) increased with increasing dietary argentine level after stress. Quadratic regression analysis (y = ?0.7147x2 + 25.986x + 147.53 R² = 0.8736) on weight gain against dietary arginine levels indicated that the optimal dietary arginine requirement was estimated to be 18.2 kg?1 of the diet (corresponding to 6.24% of dietary protein on a dry weight basis). The present study indicates that arginine has an immunostimulatory potential to help juvenile Nile tilapia cope with stress conditions such as Streptococcus iniae infection.  相似文献   

14.
The use of antibiotics to control fish disease is one of the constraints in tilapia farming. Hence, the use of probiotic and live organisms as alternative strategies has received much more attention. This study was undertaken to evaluate the use of Spirulina (Arthrospir platensis) as a growth and immunity promoter for Nile tilapia, Oreochromis niloticus (L.). Fish (1.88 g) were randomly distributed at 20 fish per 100 L aquarium and fed a diet containing either 0.0, 1.25, 2.5, 5.0, 7.5 or 10.0 g Spirulina kg?1 diet for 12 weeks. After the feeding trial, fish of each treatment were challenged by pathogenic Aeromonas hydrophila, which was given by an interperitoneal injection, and they were kept under observation for 10 days to record any abnormal clinical signs and the daily mortality rate. The growth‐promoting influence of Spirulina was observed with fish, and the optimum growth and feed utilization were obtained at 5.0 g Spirulina kg?1 diet. No significant changes were observed in fish survival among the different treatments. Spirulina supplementation improved the protein and lipid contents in fish bodies without significant differences among them, especially when fed 2.5–10.0 g kg?1 diet, but no significant differences in the moisture and ash contents were observed among different treatments. The physiological parameters were improved when fish were fed a Spirulina‐enriched diet. However, the highest red blood cells, white blood cells and nitro blue tetrazolium values were obtained at 5.0–10.0 g Spirulina kg?1 diet; meanwhile, the lowest value was obtained in the control. Total fish mortality due to A. hydrophila infection decreased with an increase in the Spirulina level in fish diets. These results indicate that Spirulina supplement is promising as an alternative method to antibiotics for disease prevention in tilapia aquaculture, and the optimum level of Spirulina in the fish diet should be 5.0–10.0 g kg?1 diet.  相似文献   

15.
A feeding trial was conducted in a closed system with Nile tilapia, Oreochromis niloticus, juveniles (mean initial weight, 2.66 g) to examine total replacement of menhaden fish meal (FM) with distiller's dried grains with solubles (DDGS), which had been used as substrate for the production of black soldier fly larvae, Hermetia illucens, in combination with soybean meal (SBM) and poultry by‐product meal (PBM), with or without supplementation of the amino acids (AA) DL‐methionine (Met), L‐lysine (Lys) and a commercial non‐amylaceous polysaccharide enzyme (Enz) product. Fish were fed seven isoenergetic [available energy (AE) = 4.0 kcal g?1 of diet] and isonitrogenous (350 g kg?1 protein as‐fed basis) practical diets formulated with equivalent digestible protein levels. Diet 1 was formulated to be similar to a commercial, high‐quality, tilapia diet containing 200 g kg?1 FM. Diets 2–5 were formulated as a 2 × 2 factorial to replace FM with similar contributions from DDGS (45%), PBM (25%) and SBM (2.1–2.9%), but to differ in supplementation of AA and/or Enz preparation. Diets 6 and 7 were formulated to investigate the effects of a 2/3 and 1/3 reduction, respectively, in DDGS contribution to the replacement protein mix, with concomitant increases in SBM, with respect to diet 3, and were balanced with Lys and Met. After 6 weeks, growth responses were slightly attenuated (P ≤ 0.05) and average daily intake (ADI) and feed conversion ratio (FCR) were slightly higher in tilapia fed DDGS diets 2–5 compared to those of fish fed the FM control diet 1. Growth responses were not significantly affected by the presence or absence of AA or Enz (diets 2–5), or the level of DDGS (diets 3, 7 and 6). Whole‐body proximate composition was not different among treatments. Amino acid profiles of fish fed DDGS diets were not significantly different from those of fish fed the FM control. Evidence of interaction between AA and Enz supplementation was detected in whole‐body amino acid concentrations such that AA content was higher with AA or Enz addition alone, but lower when both were added to the diet. Results suggest that DDGS replacement of FM in tilapia diets can be substantial when diets are formulated on a digestible protein basis and DDGS is combined with highly digestible animal (PBM) and plant proteins (SBM).  相似文献   

16.
The effect of replacing fishmeal with simple or complex mixtures of plant proteins in tilapia diets was examined. Diet formulations were arranged in a 2 × 4 factorial design with two types of plant protein mixtures used to replace fishmeal (simple: soybean meal and maize gluten meal or complex: soybean meal, maize gluten meal, dehulled flax, pea protein concentrate and canola protein concentrate) and four levels of protein originating from fishmeal (1000 g kg?1, 670 g kg?1, 330 g kg?1 and 0 g kg?1). Diets contained equal digestible protein (380 g kg?1) and digestible energy (17.6 MJ kg?1). The average daily gains, specific growth rates and feed efficiencies of fish fed diets with 0 g kg?1 fishmeal were significantly lower than fish fed diets with the 330 g kg?1, 670 g kg?1 or 1000 g kg?1 fishmeal levels. Fish fed the complex diets had significantly higher average daily gains, specific growth rates, feed : gain ratios and protein efficiency ratios than those fed the simple diets. Intestinal villus length decreased with decreasing levels of fishmeal and increased with increased diet complexity but the effects were not significant. Replacement of fishmeal with a complex mixture of plant ingredients may allow a greater replacement of fishmeal in diets fed to Nile tilapia.  相似文献   

17.
A 63‐day feeding study was conducted to evaluate the effect of partial substitution (0%, 20%, 40% and 60%) of dietary fish meal (FM) with soybean meal (SBM) on the growth performance and feed utilization of speckled shrimp, Metapenaeus monoceros juveniles. A total of 180 M. monoceros juveniles with an average weight of 0.67 ± 0.18 g were equally distributed over 12 glass aquaria (30 L each, triplicate per treatment). All aquaria were filled with filtered sea water (30–35 ‰, salinity). Four isonitrogenous (400 g kg?1 crude protein) and isocaloric (20.5 MJ GE kg?1) test diets were formulated. The control diet contained FM (D1) as the main protein source. In the other diets, FM was partially replaced with 20 (D2), 40% (D3) or 60% (D4) SBM. The results indicate that the diet in which 40% of the FM is replaced by SBM yields similar growth and production, and is as cost effective, as the control diet.  相似文献   

18.
A 16‐week feeding experiment was conducted to study the feasibility of using broad bean meal (BBM) as a replacement for fish meal (FM) for Nile tilapia Oreochromis niloticus fry, initial average weight 1.9±0.18 g. FM (50% of the diet) was used as sole sources of animal protein in the control diet 1. The replacement levels of BBM in diets (2–5) were 25%, 50%, 75% and 100% of the FM. Methionine (1%) and lysine (0.5%) were added to each diet except the control diet. Three groups of fish were fed each of five isonitrogenous (31.2% CP) and isocaloric (20.1 kJ g?1), and performance was compared against a nutritionally balanced control diet at the end of the experiment. Nile tilapia fed the diet containing 50% BBM exhibited comparable growth with those fed the FM‐based control diet. Digestibility of protein, energy and lipid decreased with increasing levels of BBM above 50% of total replacement FM into the diet. Incorporation of BBM in the diets significantly affected the moisture, fat and energy of whole fish body. These results suggest that BBM can replace 50% of the FM in diet for Nile tilapia fry, without adverse effects on fish performance.  相似文献   

19.
Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] at two inclusion levels [170 g kg?1 (L) and 350 g kg?1 (H)] were evaluated in a 13‐week experiment with triplicate groups of 92.6 ± 5.0 g gilthead seabream (Sparus aurata L.). A control diet included wheat meal, fishmeal (FM) and a mixture of plant ingredients as protein sources. Diets were formulated to be isonitrogenous and isoenergetic and processed in a twin‐screw extruder. Restricted feeding was applied (15 g kg?1 of body weight) and growth, haematology and histology parameters were evaluated. Decreased, but not significant, growth values were observed for all diets including legumes compared to the control. Poorer feed conversion ratio values were observed for both P diets and for high level B diet. Liver glycogen increased with increasing starch level, but hepatosomatic index did not differ significantly for any of the diet treatments. Histological examination of internal organs showed no pathological abnormalities that could be related to nutritional treatment. The study indicated that the tested legumes are ingredients that could be used in farmed seabream diets up to 350 g kg?1 without negative effects replacing other carbohydrate sources and part of FM.  相似文献   

20.
Two feeding experiments were carried out to evaluate the effect of partial substitution of fish meal (FM) with raw or heat‐treated cowpea Vigna unguiculata L. Walp var. IT86‐D719 seeds on growth performance, digestibility and pancreas tissue in Nile tilapia (Oreochromis niloticus L.) fry. Experiment 1 involved six isonitrogenous and isocaloric diets: four containing different concentrations of raw, whole cowpea meal (protein basis), a positive control with FM as sole protein source and a negative with cowpea meal as sole protein source. Substitution at up to 200 g kg?1 had no significant effect on production parameters, although growth was negatively affected in the negative control because of seed antinutritional factor content. Experiment 2 evaluated the effect of heat treatment (oven drying at 48 °C; hot air drying at 70 °C; or autoclaving at 119 °C) and/or seed dehulling using diets containing 200 g kg?1 cowpea meal presoaked in water. Only autoclaving eliminated trypsin inhibitor and lectin contents, independent of dehulling. Histological analysis indicated no histological changes in pancreas tissue. Raw or treated cowpea meal can replace FM in tilapia fingerling diets at up to 200 g kg?1 protein content without significantly affecting productive parameters or pancreas tissue.  相似文献   

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