共查询到20条相似文献，搜索用时 15 毫秒
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随着新技术的出现与多种技术之间的交叉融合,尿液蛋白质组学研究发展迅速。论文概述了正常的尿液蛋白质组学研究发展历程,生理情况下的尿液蛋白质组学研究进展及在尿液中已检测到疾病相关蛋白标记物的研究,列举了一些收集和整合尿液蛋白质组数据的共享平台。目前,正常尿液中的大多数蛋白种类已近被发现,建立基本的尿液蛋白质数据库非常必要的;病理状态生物标记物的检测已进入临床应用阶段,然而一种生物标记分子往往不能特异性的标记一种疾病或一个生理变化,可能需要多个相关学科的整合协调。尿液蛋白质组学研究还面临着许多困难,仍有许多工作需要去做。 相似文献
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Pallatto V Wood M Grindem C 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2005,34(4):425-428
A 6-year-old, intact male Chihuahua was presented with stranguria and painful urination of 5 days duration. Cystine crystals were observed in low numbers in unstained urine sediment preparations, and a diagnosis of cystinuria was made. Uroliths were removed surgically from the urethra and the bladder, and mineral analysis indicated the stones were composed of 100% cystine. Cystinuria results from an inherited defect in renal tubular transport of cystine that affects many breeds and has been found as an autosomal recessive trait in Newfoundlands. Accurate identification of cystine crystals in urine is an important means of diagnosing cystinuria. 相似文献
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犬尿结石的临床诊治体会 总被引：1，自引：0，他引：1
犬尿结石是临床上经常遇到的一种严重的泌尿系统疾病。它是由尿中的无机盐析出形成结石,引起尿路粘膜发炎、出血和排尿障碍的疾病的总称。根据尿结石形成和阻塞部位的不同,可分为肾盂结石、输尿管结石、膀胱结石和尿道结石。该病多发生于老 相似文献
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Urine collection harness for horses 总被引：1，自引：0，他引：1
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ADJ WATOSON 《Australian veterinary journal》1998,76(6):392-398
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Ilse K. van Vonderen Hans S. Kooistra Ad Rijnberk 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1997,11(1):30-35
Urine specific gravity (Usg) and urine osmolality (Uosm) are used routinely to assess renal concentrating ability, but limited data on these variables are available for healthy dogs. Consequently, we studied the intra- and interindividual variations in Usg and Uosm in healthy dogs as well as the influence of age and gender on these variables. Dogs were selected for health and anestrus in female dogs through the use of a detailed questionnaire. Eighty-nine owners collected morning and evening urine samples from their dogs on 2 consecutive days. In 8 dogs in which the Uosm of different samples varied more than 50%, owners collected urine for 24 hours at 2-hour intervals during the day and at 4-hour intervals at night. The possible effect of changes in adrenocortical function with age was assessed by measurements of urinary corticoid/creatinine (C/C) ratios. Among all samples, Uosm ranged from 161 to 2,830 mOsm/kg and Usg from 1.006 to > 1.050. In the morning, Uosm (1,541 ± 527 mOsm/kg, range 273–2,620 mOsm/kg) and Usg (1.035 ± 0.010, range 1.009- > 1.050) were higher than in the evening (Uosm 1,400 ± 586 mOsm/kg, range 161–2,830 mOsm/kg; Usg 1.031 ± 0.012, range 1.006- > 1.050). The interindividual coefficient of variation in Uosm was 34.2% for morning urine samples and 41.9% for evening samples. In 8 dogs with large differences in urine concentration, there were 2– to 3-fold increases or decreases in Uosm during the day, and the intraindividual coefficient of variation was 33.0%. There was no relation between gender and urine concentration. Urine concentration in both the morning and evening samples decreased with age. Urinary corticoid/creatinine ratios did not change with age. It can be concluded that Uosm and Usg vary widely among healthy dogs. Urine concentration is generally lower in the evening than in the morning and is not related to gender. Urine concentration decreases with age, and this cannot be ascribed to an associated increase in endogenous corticoids. In some dogs, Uosm varies widely during the day, with an intraindividual coefficient of variation approaching the interindividual coefficient of variation. This may be regarded as a biologic variation but also could represent an early undi-agnosed clinical abnormality. 相似文献
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Gregory F. Grauer Deborah S. Greco Ellen N. Behrend Indu Mani Martin J. Fettman Timothy A. Allen 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(5):324-327
The correlation between 24-hour urinary excretion of N -acetyl-β- d -glucosaminidase (NAG) and γ-glutamyl transferase (GGT) with urine NAG and GGT/creatinine ratios was assessed in dogs with gentamicin-induced nephrotoxicosis. Eighteen 6-month-oid male Beagles with normal renal function were randomly divided into 3 groups of 6. Each group was fed a different concentration of protein (high protein, 27.3%; medium protein, 13.7%; and low protein, 9.4%) for 21 days. After dietary conditioning, gentamicin was administered at a dose of 10 mg/kg IM tid for 8 days and each group was continued on its respective diet. Endogenous creatinine clearance and 24-hour urinary excretion of NAG and GGT were determined after dietary conditioning (day 0) and on days 2, 4, 6, and 8 of gentamicin administration. In addition, urine NAG and GGT/creatinine ratios (IU/L ± mg/dL) were determined from catheterized spot urine samples obtained between 7 and 10 am on the same days. The correlation between 24-hour urinary enzyme excretion and urine enzyme/creatinine ratio in the spot urine samples was evaluated by simple linear regression analysis. Spot sample urine enzyme/creatinine ratios were significantly correlated with 24-hour urinary enzyme excretion through day 4 for dogs on low dietary protein, through day 6 for those on medium protein, and through day 8 for those on high dietary protein. Mean ± SD baseline values for urine NAG/creatinine ratio and 24-hour urinary NAG excretion were 0.06 ± 0.04 and 0.19 ± 0.14 IU/kg/24 hr, respectively. Baseline values for urine GGT/creatinine ratio and 24-hour urinary GGT excretion were 0.39 ± 0.18 and 1.42 ± 0.82 IU/kg/24 hr, respectively. 相似文献
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猪尿中克伦特罗检测方法--酶联免疫吸附测定法 总被引：4，自引：2，他引：2
农业部于2001年11月1日发布农牧发[2001]38号文，发布10种动物源食品中兽药残留检测方法。方法之十：猪尿中克伦特罗检测方法——酶联免疫吸附测定法。全文如下：猪尿中克伦特罗检测方法——酶联免疫吸附测定法1范围本标准规定了猪尿中克伦特罗检验的制样和酶联免疫吸附测定方法。本标准适用于猪尿中克伦特罗的残留常规快速筛选检测。2规范性引用文件下列文件中的条款通过本标准的引用而成为本标准的条款。凡是注日期的引用文件，其随后所有的修改单(不包括勘误的内容)或修订版均不适用于本标准，然而，鼓励根据本标准达成协议的各方研究是… 相似文献
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为了调查上海屠宰生猪盐酸克伦特罗(瘦肉精)的残留情况,2010—2011年对该市4个定点屠宰场分25个批次,共取猪尿样本2 791份,用ELASA进行快速筛检,结果显示:阳性率为4.73%(132/2 791)。 相似文献
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As hydrocortisone is an endogenous substance, it is first necessary to establish its normal concentrations so as to be able to control its use in racing animals. This study was designed to establish the hydrocortisone concentrations in post-race urine samples of horses racing in Brazil and also to evaluate the results in relation to the international threshold set for this drug. Urine samples were analysed by HPLC-UV. The results were evaluated according to the concentration range as well as sex and time of sample collection (afternoon or evening races). The results showed a high degree of variation in the concentrations of hydrocortisone in the urine (93±69 ng/ml). The maximum concentration observed was 646 ng/ml, although only a few horses (around 1%) showed levels within the range 500–650 ng/ml, 91% being in the range 0–150 ng/ml. The data suggested a normal distribution curve. Statistical analysis showed no significant influence of sex or time of sample collection. 相似文献