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1.
全反式维甲酸对大鼠前体脂肪细胞增殖与分化的影响 总被引：3，自引：0，他引：3
利用MTT比色检测不同浓度的全反式维甲酸(all trans Retinoic Acid ATRA)对大鼠前体脂肪细胞增殖的影响,采用油红O染色和吖啶橙染色观察大鼠前体脂肪细胞分化的形态变化;采用油红O染色提取法分析不同浓度ATRA对大鼠前体脂肪细胞分化的影响。MTT比色结果显示,低浓度(10-8～10-7mol/L)的ATRA对前体脂肪细胞的增殖具有促进作用(P<0101),而高浓度(10-6～10-4mol/L)的ATRA则抑制前体脂肪细胞的增殖(P<0101)。油红O染色和吖啶橙染色结果表明,前体脂肪细胞分化成脂肪细胞后,细胞内充满脂滴,由梭形变成椭圆形;油红O染色提取法结果显示高浓度(10-6～10-4mol/L)的ATRA能够显著抑制前体脂肪细胞的分化(P<0101),而低浓度(10-8～10-7mol/L)的ATAR却显著促进前体脂肪细胞的分化(P<0101)。 相似文献
2.
Höinghaus R Hewicker-Trautwein M Mischke R 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(1):104-111
BACKGROUND: Immunocytochemical techniques are a potentially valuable diagnostic tool to support cytologic diagnosis in dogs. However, detailed studies of staining patterns and intensity in cytologic specimens of mesenchymal tumor types are lacking. OBJECTIVE: The aim of this study was to evaluate commercially available antibodies against human proteins for use in the characterization of canine tumors of mesenchymal origin in cytologic samples. METHODS: Immunocytochemical staining was performed on air-dried imprint specimens of biopsies obtained from 103 mesenchymal neoplasms and 14 metastatic lesions from 98 dogs. All specimens were stained with anti-cytokeratin AE1/AE3 and vimentin. Based on the histologic diagnosis, tumors of muscle, endothelial, histiocytic, and melanocytic origin also were stained with cell-specific antibodies. Staining intensity was subjectively graded and the percentage of positive tumor cells was estimated. RESULTS: All mesenchymal tumors and metastases, with the exception of mesotheliomas, were vimentin-positive and cytokeratin-negative; mesotheliomas (n=6) were positive for both vimentin and cytokeratin. Tumors of muscle (n=5), endothelial (n=15), and histiocytic (n=18) origin stained moderately to strongly positive in a majority of tumor cells with desmin, von Willebrand factor, and lysozyme, respectively. Malignant melanomas (n=15) had variable staining and a variable percentage of positive cells with Melan-A and S100. CONCLUSIONS: Our results indicate that immunocytochemical staining of canine cytologic specimens is a reliable and sensitive technique that may be of benefit for the differentiation of poorly differentiated mesenchymal tumors and metastases. Additional study is needed to assess the specificity of immunocytochemical stains in mesenchymal tumors. 相似文献
3.
Brown JS Johnson MC Sims WP Boone LH Swor TM Weeks BR 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(2):265-269
A 4-year-old Quarter Horse mare was presented to the Texas A&M University Veterinary Medical Teaching Hospital for evaluation of a rectal tear. On initial evaluation, rectal palpation and colonoscopy revealed a grade IIIb rectal tear. Analysis of peritoneal fluid revealed a modified transudate. Preliminary supportive care included fluid therapy and mineral oil administration via nasogastric tube. Approximately 48 hours after presentation, a second abdominocentesis was performed, and cytologic examination of the fluid revealed a marked suppurative exudate. Round clear nonrefractile material observed within neutrophils and macrophages and in the background stained bright pink to red with Oil Red O, confirming the material as lipid, likely from leakage of mineral oil through the rectal tear. The condition of the mare deteriorated and euthanasia was elected due to the poor prognosis. At necropsy, gross and histologic findings included peritoneal effusion and a full-thickness rectal tear with transmural necrotizing pyogranulomatous colitis and fibrinous peritonitis. To the authors' knowledge, this is the first reported case of Oil Red O-positive lipid vacuoles in the peritoneal fluid of a horse from presumed leakage of mineral oil through a transmural rectal perforation. The frequency of this occurrence in horses is unknown, but it is important for cytopathologists to be familiar with the appearance and significance of lipid-type droplets in phagocytic cells in cytologic fluid analysis specimens. 相似文献
4.
Chang SC Liao JW 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):637-640
We report a liposarcoma at the mesojejunoileum that was widely excised, and then the intrahepatic metastatic liposarcoma was found. The dog was treated by two single-agent chemotherapy separately. The patient was an 8-year-old, male Dachshund, and was referred to our hospital owing to the significant distention of the left abdomen. Neither radiography nor ultrasonography detected the actual association of the tumor with the abdominal viscera before surgery. A large-sized tumor mass that adhered to the mesojejunoileum was explored by laparatomy. Final diagnosis of a pleomorphic liposarcoma was made by the routine histology and further identified by Oil Red O stain. The two single-agent chemotherapy including doxorubicin followed by capecitabine, however, failed to cause remission of the intrahepatic metastatic lesions. The patient died 22 months after operation and the cause of death was supposed to be metastatic liposarcoma. 相似文献
5.
Characterization of melamine-containing and calcium oxalate crystals in three dogs with suspected pet food-induced nephrotoxicosis 总被引：16，自引：0，他引：16
Thompson ME Lewin-Smith MR Kalasinsky VF Pizzolato KM Fleetwood ML McElhaney MR Johnson TO 《Veterinary pathology》2008,45(3):417-426
The histomorphologic characteristics and chemical composition of the crystals associated with suspected pet food-induced nephrotoxicosis in 3 dogs are described. Kidney specimens from 2 dogs, a 3-year-old Parson Russell Terrier and a 3-year-old Bernese Mountain Dog, were examined. Both developed acute renal failure after eating canned pet food on the 2007 Menu Foods recall list. The third case was a kidney specimen from a 1-year-old mixed-breed dog from a similar 2004 outbreak of canine renal failure in Taiwan, which occurred after eating a commercial dog food. Hematoxylin and eosin (HE), 72-hour Oil Red O (ORO72h), Alizarin Red S (pH 4.1-4.3), and Von Kossa stains; infrared (IR) spectroscopy; and scanning electron microscopy with energy dispersive X-ray analysis (SEM/EDXA) were performed to determine the histomorphologic characteristics and chemical composition of the crystals observed in each case. Histomorphologic findings in each case included acute, marked tubular degeneration and necrosis with many intratubular birefringent crystals, and lymphoplasmacytic interstitial nephritis. In each case, most of the crystals were rough, pale brown, and stained with ORO72h but did not stain with Alizarin Red S (pH 4.1-4.3) or Von Kossa stains; these features were consistent with a plastic or lipid. IR spectroscopy and SEM/EDXA results were consistent with melamine-containing crystals. A second crystal type identified in each case was smooth and platelike with staining characteristics and IR spectroscopy and SEM/EDXA results consistent with calcium oxalate crystals. Melamine-containing crystals have distinct light microscopic, histochemical, and SEM/EDXA characteristics that facilitate their identification in tissue. 相似文献
6.
Skin biopsies from three groups of sheep infested with Psoroptes ovis were cryofixed in liquid nitrogen to preserve outer epidermis with its lipid. From one group of five sheep (Group A), biopsies were taken from relatively healthy skin near the edge of scab lesions where mites had congregated. Frozen vertical sections from these biopsies were stained with Oil Red O and haematoxylin before mounting. Red lipid globules were plentiful within the body cavity of sectioned mites, but ingested lipid could not be distinguished from endogenous mite body stores by this technique. In a second group of five sheep (Group B), enclosed lumbar skin areas were coloured red with the lipophilic stains Oil Red O or Sudan IV. These enclosed coloured skin areas were inoculated with P. ovis and sampled by skin biopsy 1 or 4 days later. Cryofixed biopsies were cut into vertical frozen sections and mounted without staining for examination. Red-coloured lipid within mites, matching red-coloured lipid on outer epidermis, was evidence for the epidermal origin of P. ovis ingesta in the early stages of an infestation. From two other sheep (Group C), cryofixed biopsies were examined by scanning electron microscope and mites were seen with mouthparts embedded in abraded outer epidermis, but the precise depth of epidermal penetration was not determined. A light microscope survey of 3198 frozen vertical skin sections from the first 10 sheep (Groups A and B) showed that inner stratum corneum of the epidermis was the deepest penetration recorded for gnathosomes of P. ovis cryofixed in situ by liquid nitrogen. No structure of P. ovis was identified in dermal tissues. 相似文献
7.
旨在研究RNF20及其介导的组蛋白H2B第120位赖氨酸的单泛素化（H2Bub）对小鼠棕色脂肪细胞成脂分化的影响。采集1日龄和2月龄雄性C57BL/6小鼠的棕色脂肪组织（n=3），用Western blot方法检测RNF20的表达及其介导的H2Bub水平。利用胶原酶消化法分离获得1日龄小鼠的棕色前体脂肪细胞。分别诱导棕色前体脂肪细胞和C3H10T1/2细胞系成脂分化，通过油红O染色检测其分化效果，进一步通过Western blot检测细胞分化前后（0和8 d）RNF20的表达及其介导的H2Bub水平。通过siRNA干扰Rnf20基因在C3H10T1/2细胞系中的表达，油红O染色方法观察Rnf20基因对成脂分化的影响，利用qPCR和Western blot技术检测Rnf20基因的干扰效率及其介导的H2Bub水平。结果显示，2月龄小鼠棕色脂肪组织中RNF20表达量及其介导的H2Bub水平均显著高于1日龄小鼠。脂肪细胞分化标记蛋白PPARγ和CEBPα的表达水平，RNF20表达量及其介导的H2Bub水平在棕色前体脂肪细胞及C3H10T1/2细胞成脂分化后均显著增加。此外，在C3H10T1/2细胞中敲降Rnf20基因后，与阴性对照组相比，RNF20及其介导的H2Bub水平显著降低，成脂分化后脂滴明显减少。综上表明，RNF20对小鼠棕色脂肪细胞的分化是必需的，敲降Rnf20基因导致组蛋白H2Bub水平显著降低，且降低了C3H10T1/2细胞的成脂分化效率。本研究丰富了小鼠棕色脂肪细胞分化过程中的表观遗传调控研究，为深入理解动物脂肪细胞分化提供了新的基因素材。 相似文献
8.
A 5-year-old, female, Japanese Macaque (Macaca fuscata) was diagnosed with round cell variant of myxoid liposarcoma. At necropsy, multifocal to coalescing, reddish tan to white nodules, ranging from 0.5 to 1 cm in diameter, were noted throughout the omentum and retroperitoneum. Similar neoplastic nodules were also present in diaphragm, abdominal wall, and on hepatic capsule. Microscopically, neoplastic masses consisted of round to polyhedral cells, which had round, often eccentric nuclei and abundant eosinophilic granular and microvacuolated cytoplasm; Oil red O staining demonstrated large numbers of lipid droplets in the cytoplasm. Ultrastructurally, the cytoplasm of the tumor cells was packed with occasional lipid vacuoles and numerous enlarged mitochondria. Immunohistochemistry revealed tumor cells were positive for vimentin, while negative to cytokeratin, actin, and Factor VIII-related antigen. To the authors' knowledge, this is the first report of round-cell variant of myxoid liposarcoma in nonhuman primate. 相似文献
9.
本研究旨在探讨C型利钠肽（CNP）对鸡胸肌组织肌内前脂肪细胞的增殖、分化和分解的调控作用及机制，为进一步阐明肌内脂肪沉积的分子机制奠定基础。以黄羽肉鸡胸肌组织的肌内前脂肪细胞作为体外研究模型，利用10-7 mol/L CNP激素外源诱导前脂肪细胞，采用CCK8、Edu染色法观察肌内前脂肪细胞增殖的变化，油红O染色和异丙醇萃取法观察脂肪分化和沉积的变化；利用试剂盒检测细胞内cGMP及甘油浓度变化；实时荧光定量PCR检测利钠肽受体A、B、C（NPRA、NPRB和NPRC）的表达变化。结果显示，与对照组相比，CNP诱导前脂肪细胞1和3 d后，细胞的增殖能力显著升高（P<0.05）；CNP诱导3和6 d后，诱导组脂肪细胞的分化和脂滴沉积能力极显著降低（P<0.01），释放到培养基的甘油浓度及细胞中的cGMP的浓度极显著升高（P<0.01）。NPRB和NPRC基因mRNA的表达极显著和显著高于对照组（P<0.01；P<0.05），NPRA基因mRNA的表达无显著变化（P>0.05）。本试验结果表明，CNP通过NPRB/NPRC-cGMP通路调控肉鸡肌内脂肪细胞的脂代谢过程。 相似文献
10.
HUANG Huayun LIANG Zhong LIU Longzhou ZHAO Zhenhua WANG Qianbao LI Chunmiao HUANG Zhenyang ZHANG Xin LI Shoufeng 《中国畜牧兽医》2007,47(11):3555-3562
The aim of this study was to investigate the regulatory effect and mechanism of C-type natriuretic peptide (CNP) on the proliferation,differentiation and lipolysis of intramuscular preadipocytes (IMPs) in breast muscle of chickens,and lay a foundation on further elucidating the molecular mechanism of intramuscular fat deposition in chickens.The IMPs of breast muscle in Yellow-feathered broilers were used as the model in vitro,the preadipocytes were induced by 10-7 mol/L CNP.The proliferation,differentiation and lipolysis of IMPs were observed by CCK8,Edu staining,Oil Red O staining and isopropanol extraction,respectively.The concentration of cGMP and glycerin were detected by cGMP and glycerin kit,respectively.The expression of NPRA,NPRB and NPRC genes mRNA was detected by Real-time quantitative PCR.The results showed that compared with control group,the cell proliferation in CNP treatment group was enhanced significantly at 1 and 3 d (P<0.05).The cell differentiation and lipid deposition were extremely significantly decreased at 3 and 6 d (P<0.01),the intracellular cGMP concentration and glycerin concentration in the medium at 3 and 6 d were extremely significantly increased (P<0.01).The expression of NPRB and NPRC genes mRNA in CNP treatment group was extremely significantly or significantly higher than control group (P<0.01;P<0.05),and the expression of NPRA gene mRNA had no significant difference (P>0.05).The results revealed that CNP regulated the lipid deposition of intramuscular adipocytes through NPRB/NPRC-cGMP pathway in chickens. 相似文献
11.
Simeonov R Simeonova G 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):88-90
BACKGROUND: The use of computer-based image analysis systems in veterinary oncology has increased. Computerized morphometry is a part of image analysis that describes geometric figures of cellular structures in any dimension. Most investigators have performed morphometric analysis on histologic specimens. Computer-assisted nuclear cytomorphometry can provide important preoperative information on neoplastic lesions in animals. OBJECTIVES: The aim of this study was to define whether the morphometric parameters of mean nuclear diameter and nuclear roundness could be used to differentiate benign from malignant canine mammary gland tumors on cytologic specimens. METHODS: Mean nuclear diameter and nuclear roundness were determined by computer-assisted morphometry of epithelial cells in Hemacolor-stained cytologic smears from normal canine mammary gland (n = 7) and from canine mammary adenomas (n = 8), tubulopapillary carcinomas (n = 9), and solid carcinomas (n = 6). Data were analyzed by the Mann-Whitney U test. RESULTS: Significant differences (P <.001) were found in mean nuclear diameter and nuclear roundness among all tumor types and in comparison with normal canine mammary gland epithelial cells (except for nuclear roundness between tubulopapillary carcinomas and solid carcinomas). CONCLUSIONS: The morphometric parameters of mean nuclear diameter and nuclear roundness can be used in the preoperative differentiation of benign from malignant canine mammary gland tumors. 相似文献
12.
旨在探究产蛋各期番鸭肝腺苷酸活化蛋白激酶（AMPK）信号通路基因表达和肝脂肪酸组成,为番鸭肝脂质代谢应答产蛋提供机理研究。本研究选取开产前22周龄、产蛋初期30周龄、产蛋中期40周龄和产蛋末期60周龄母番鸭各15羽,全自动生化仪测定血脂水平,苏木精-伊红（HE）染色和油红O染色观察肝组织学结构,实时荧光定量PCR （qRT-PCR）检测肝AMPK通路基因表达,气相色谱-质谱联用法（GC-MS）检测产蛋各期肝脂肪酸组成。结果表明,总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、甘油三酯（TG）和极低密度脂蛋白胆固醇（VLDL-C）水平在40周龄显著高于22、30和60周龄（P<0.05）;高密度脂蛋白胆固醇（HDL-C）水平在30和40周龄显著低于22和60周龄（P<0.05）。肝HE和油红O染色切片显示,肝在22周龄呈实质状,至产蛋40和60周龄,肝脂滴沉积明显（P<0.05）。AMPKα1在22、30、40和60周龄呈本底低水平表达,并显著低于产蛋各期肉碱脂酰转移酶1（CPT1）和脂肪酸合成酶（FAS）的表达量（P<0.05）,FAS在22、40和60周龄均呈高水平表达（P<0.05）;羟甲基戊二酰辅酶A还原酶（HMGR）、肝细胞核因子4α（HNF4α）、乙酰辅酶A羧化酶1（ACC1）和固醇调控元件结合蛋白-1（SREBP1c）在40周龄表达量显著高于22和30周龄（P<0.05）。肝中饱和脂肪酸（SFA）、单不饱和脂肪酸（MUFA）和多不饱和脂肪酸（PUFA）主要由C16∶0、C18∶0、C18∶1和C20∶2 n-6构成,分别占总脂肪酸含量的32%、16%、30%和9%。C14∶0和C16∶0含量在40周龄显著高于22周龄（P<0.05）;C24∶0、C20∶2 n-6和PUFA含量在60周龄显著高于22和40周龄（P<0.05）。综上,番鸭产蛋期肝通过上调FAS等脂质合成基因表达,合成大量长链脂肪酸,沉积于肝,并增加血脂水平。 相似文献
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Adiponectin is a protein hormone secreted exclusively by adipocytes that plays an important role in the modulation of glucose and lipid metabolism. To investigate the effect of adiponectin on lipid metabolism in chicken, rosiglitazone (agonist of adiponectin) and dexamethasone (inhibitor of adiponectin) were used to treat 23‐day‐old broilers in vivo. To verify the functionality of adiponectin on fat deposition, chicken pre‐adipocytes were cultured in the medium containing 10 μg/ml adiponectin. Serum adiponectin and lipids and fat distribution were analysed. Oil Red O staining was used to determine lipid deposition in adipocytes. The expression levels of adiponectin, adiponectin receptors (AdipoR) and lipid metabolism–related genes in different tissues and pre‐adipocytes were measured using real‐time PCR, and the abundance of lipid metabolism–related proteins was measured by Western blot. Rosiglitazone increased serum adiponectin concentration and the expression levels of adiponectin and adiponectin receptor 1 (AdipoR1) in tissues and significantly decreased levels of serum lipids and fat deposition. Rosiglitazone significantly increased the expression levels of adipose triglyceride lipase (ATGL) and AdipoR1 and decreased the expression levels of fatty acid synthase (FAS). Dexamethasone had the converse effects compared with rosiglitazone. Oil red O staining results showed a marked decrease in fat deposition in cells treated with adiponectin. In adipocytes, adiponectin could decrease the expression levels of CCAAT/enhancer‐binding protein α (C/EBPα) and FAS and increased the expression levels of ATGL and AdipoR1. These results indicate that adiponectin has a remarkable effect on impairment of adipocyte differentiation, which contributes to the negative regulation of fat deposition in chicken. 相似文献
14.
miR-106b-5p靶向KLF4调控山羊肌内前体脂肪细胞分化 总被引：1，自引：1，他引：0
旨在明确miR-106b-5p对山羊肌内前体脂肪细胞分化的影响，并确定这种作用是通过靶向KLF4来实现的。本研究利用实时荧光定量PCR （quantitative real-time PCR，qRT-PCR）技术检测miR-106b-5p在山羊肌内前体脂肪细胞分化过程中的表达模式，通过脂质体转染技术将miR-106b-5p mimic和miR-106b-5p inhibitor转入体外培养的山羊肌内前体脂肪细胞，油红O染色法从形态学验证miR-106b-5p对脂肪细胞中脂滴积聚的影响，qRT-PCR检测预测的靶标基因KLF4和脂肪分化标志基因的表达情况，利用双荧光素酶报告系统鉴定miR-106b-5p与KLF4的靶标关系。qRT-PCR结果显示，miR-106b-5p在山羊肌内前体脂肪细胞诱导分化第3天时表达量最高。在山羊肌内脂肪细胞中干扰miR-106b-5p后油红O染色显示脂滴聚积减少，过表达miR-106b-5p后脂滴聚积增加。在山羊肌内前体脂肪细胞中转染miR-106b-5p inhibitor后PPARγ表达量显著降低（P<0.05），而KLF4表达量极显著升高（P<0.01）；转染miR-106b-5p mimic后LPL和PPARγ表达量极显著升高（P<0.01）。荧光素酶活性试验结果显示，过表达miR-106b-5p可显著抑制KLF4荧光活性。miR-106b-5p通过靶向并负调节KLF4的表达促进山羊肌内脂肪细胞分化。 相似文献
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16.
The objectives of this study were to evaluate morphologic, histochemical, and immunohistochemical characteristics of well-differentiated and anaplastic intraocular neoplasms of cats, and to develop a diagnostic algorithm for, and investigate the association of ruptured lenses with these neoplasms. Seventy-five feline globes with intraocular neoplasms were stained with hematoxylin and eosin and examined by light microscopy. Morphologic diagnoses included 33 intraocular sarcomas, 17 diffuse iris melanomas, 15 lymphosarcomas, three ciliary adenomas, one metastatic carcinoma, and six undifferentiated intraocular neoplasms. Sections of these globes were then stained with periodic acid Schiff (PAS), and immunohistochemical (IHC) labels for various cellular markers. Histochemical staining and IHC labeling confirmed cellular differentiation in 73/75 neoplasms but was discordant with morphologic diagnoses in 8/75. These included four neoplasms morphologically diagnosed as lymphosarcomas but which expressed differentiation antigens consistent with melanoma (n = 3) or ciliary adenocarcinoma (n = 1), and four tumors morphologically diagnosed as intraocular sarcomas that expressed differentiation antigens for melanoma (n = 2), metastatic carcinoma (n = 1), or remained undifferentiated (n = 1). Immunohistochemical labeling suggested a diagnosis in 5/6 morphologically undifferentiated neoplasms including one intraocular sarcoma, two diffuse iridal melanomas, and two ciliary adenocarcinomas. Based upon morphologic, histochemical, and IHC characterization, ruptured lens capsules were detected in 28/30 intraocular sarcomas, 3/24 diffuse iris melanomas and 1/11 lymphosarcomas, but not in ciliary epithelial neoplasms, metastatic carcinomas, or undifferentiated intraocular neoplasms. An algorithm is provided that facilitates stain and IHC label selection for differentiating anaplastic intraocular feline neoplasms. 相似文献
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Boyd SP Taugner FM Serrano S Gregory SP Tennant KV 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2005,34(3):271-274
A 5-year-old, intact male Italian Spinone dog was presented for progressive, severe dyspnea and coughing. Thoracic radiographs revealed a large mass in the right cranial thorax. Fine needle aspiration of the mass yielded a highly cellular sample containing dense clumps of oval to spindle-shaped mesenchymal cells with distinct intracytoplasmic vacuolation, consistent with lipoblasts and lipocytes. Cell clusters were associated with abundant eosinophilic matrix, which was identified as mucin, based on Alcian blue staining. At exploratory thoracotomy, the mass was found to be nonresectable, and the dog was euthanized. Histologic sections of the multilobular mass had discrete regions of variable cellular differentiation, including highly cellular areas of pleomorphic cells, areas of spindle cells and lipoblasts in a myxoid background, and areas of well-differentiated lipogenic cells. The histologic diagnosis was myxoid liposarcoma. The thoracic cavity is a rare site for liposarcoma in the dog. The cytologic features of lipoblasts together with a mucopolysaccharide matrix were useful for distinguishing the myxoid variant of liposarcoma from other forms of liposarcoma and myxoid sarcomas. 相似文献
19.
Bauer NB Zervos D Moritz A 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(5):928-935
BACKGROUND: The count of argyrophilic nucleolar organizing regions (AgNOR) has been considered a useful variable that reflects cellular proliferation in canine lymph nodes, but it has not been compared with other markers of proliferation. Hypothesis: Ki67 and AgNORs are equally useful as markers of tissue proliferation in fine needle aspirates of canine lymph nodes. ANIMALS: A total of 101 dogs. MATERIAL AND METHODS: Prospective, observational study of a convenience sample of dogs. Two smears were prepared for a May-Gruenwald-Giemsa stain and a Ki67/AgNOR double stain. In addition, CD3/CD79a immunostaining was performed when cytologic examination revealed a lymphoma. The dogs were grouped as normal (n = 26), reactive hyperplasia (n = 25), lymphadenitis (n = 31), and lymphoma (n = 19), based on the physical examination and the cytologic findings. The AgNOR count/cell, AgNOR area/cell and the percentage of cells staining positive for Ki67 were evaluated in 100-167 cells (median, 113 cells) by using automatic image analysis. RESULTS: Mean (SD) AgNOR counts/cell were 1.36 +/- 0.19 in normal dogs, 1.55 +/- 0.26 in lymphadenitis, 1.65 +/- 0.32 in reactive hyperplasia, and 3.67 +/- 1.08 in lymphoma. The percentage of Ki67 positive cells was 2.67 +/- 0.99% in normal lymph nodes, 5.04 +/- 3.34% in lymphadenitis, 5.36 +/- 2.14% in reactive hyperplasia, and 30.2 +/- 10.8% in lymphoma. All variables were significantly higher in dogs with lymphoma compared with the other groups (P < .0001). The sensitivity and the specificity of the AgNOR count for diagnosing lymphoma were 95 and 96% at a cutoff value of >2.04 AgNORs/cell. The cutoff value for the Ki67 positive cells was >10.40% (sensitivity, 95%; specificity, 98%). CONCLUSION AND CLINICAL IMPORTANCE: The results indicated that both AgNOR and Ki67 counts were good diagnostic tools for assessment of proliferation in aspirates of canine lymph nodes. 相似文献
20.
G K Ogilvie H A Reynolds R C Richardson S J Withrow A M Norris R A Henderson J S Klausner J D Fowler D McCaw 《Journal of the American Veterinary Medical Association》1989,195(11):1580-1583
One hundred eighty-five dogs with histologically confirmed, measurable malignant tumors were used in a prospective study to determine the response to 2 doses of the anthracycline antitumor antibiotic, doxorubicin. Eighty-three dogs had been refractory to one or more previous treatment modalities (surgery, n = 54; chemotherapy, n = 22; radiation, n = 10; hyperthermia, n = 1; biological response modifier, n = 1). The extent of neoplastic disease was determined immediately prior to and 3 weeks after 2 doses of doxorubicin were administered (30 mg/m2 of body surface area, iv) 21 days apart. Eighty-four percent (n = 157) of the dogs received 2 doses of doxorubicin and were evaluated. Of the 28 dogs ruled ineligible, 4 had serious side effects to the first dose of doxorubicin, and 24 others acquired complications resulting from their malignant tumors. A partial or complete remission was obtained in 41% (64/157) of all evaluable dogs: 26% (11/43) of the dogs with carcinoma, 67% (42/63) of the dogs with lymphoma, and 22% (11/51) of the dogs with sarcoma. Tumors in which there was at least a 50% volume reduction (partial or complete remission) included malignant lymphoma (42/63), fibrosarcoma (1/14), solid follicular thyroid carcinoma (3/13), mammary adenocarcinoma (2/8), hemangiosarcoma (2/8), osteosarcoma (1/6), circumanal carcinoma (3/5), synovial cell sarcoma (2/3), undifferentiated sarcoma (2/3), nasal adenocarcinoma (1/2), liposarcoma (1/2), infiltrating lipoma (1/1), malignant melanoma (1/1), sclerosing mesothelioma (1/1), and neurofibrosarcoma (1/2).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献