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1.
Summary. High concentrations of 2,4-D and picloram interfered with the downward movement of 14c-assimilates infield-grown vines. The interference in translocation was appreciably greater with picloram than it was with 2,4-D, Although basipetal translocation was retarded, translocation within the treated shoots continued from the vegetative part to the clusters. Translocation of 2,4-D appeared to follow the same route as 14c-assimilatcs for the most part. Formative effects were absent on untreated grape shoots although the adjacent shoots treated with 2,4-D or picloram on the same cordons were killed; however, formative effects were evident on some of the stump sprouts which developed after the vines were harvested. The malformed leaves on the stump sprouts were twelve or more nodes from the base of the shoots, while 14c was in the more basal leaves.
Thompson Seedless (Sultanina) rootings treated with 20 000 ppm 2,4-D or picloram transported less 14C to the roots than did the controls. Treatment with either herbicide resulted in a marked increase in the labelling of the stems.
Effet du 2,4-D et du pichlorame sur la migration de métabolites marqués au 14C dans Vitis vinifera L.  相似文献   

2.
Summary. Translocation of 3-amino-1,2,4-triazole-5-14C (14C-aminotriazole) was compared to that of 14C-assimilates in couch grass ( Agropyron repens (L.) Beauv.) at three different growth stages.
Assimilates of 14CO2 were translocated from the treated shoot to other shoots and rhizomes at the 2–3-leaf and 3–4-leaf stages of development. Much less labelled material was translocated into untreated shoots at the 5-leaf stage. More 14C-assimilates were translocated to the roots than to untreated shoots at all developmental stages. The translocation patterns of 14C-aminotriazole and 14C-assimilates were similar.
Two metabolites, A and B, were formed from 14C-aminotriazole, which chromatographed identically to previously described metabolites in Cirsium arvense (L.) Scop. A was further metabolized into B. Labelled aminotriazole and its two metabolites were translocated throughout the plants. Metabolite A was phytotoxic when concentrated and re-applied to couch grass, but its properties were not those of Unknown II from Cirsium arvense. They were the same as those of Unknown III. Whether or not metabolite A and Unknown III are identical was not established.
Migration des substances assimilables marquées au 14 C, du 3 amino-1,2,4-triazole et de ses métabolites chez Agropyron repens  相似文献   

3.
Summary. Previous findings suggested that the translocation in Agropyron repens of 2,2-dichloropropionic acid (dalapon) may be considerably affected by the transpiration rate. This relationship has been studied further in the present investigation using 14C-labelled material and autoradiography.
It was found that when dalapon was applied to the leaves and the treated plants were placed in darkness a reduction in the transpiration rate of ca. 90% was associated with a marked increase in the amount of dalapon translocation into the roots and tillers. The evidence provided by the autoradiographs was confirmed by a quantitative assay of the 14C present in the roots. It was also found, however, that translocation was not appreciably affected either by a 50% reduction in the light intensity or when the transpiration rate was reduced by placing the plaints under conditions of high humidity in the light or by the application of white petroleum jelly to the leaves. An alternative hypothesis, namely that the effect of darkness on the movement of the herbicide might be due to changes induced in the normal pattern of assimilate translocation was also investigated but was not supported by the results obtained. Further experimentss, in which various parts of the treated shoot were placed in the dark, suggested that the effect of darkness on the translocation of dalapon is exerted primarily on the treated leaf itself.
Etudes sur to migration dans Agropyron repens de l'acide 2,2-dichloropropionique marqué avec 14C  相似文献   

4.
Summary. 14CO2 together with autoradiographic techniques was used to stuy the movement of labelled carbon within wheat plants at different stages in their development. Movement of 14C about the plain was found to be extensive in the early stages, gradually bccoming more localized until at a stage after ear emergence there was little transference of labelled carbon between tillers within a 24-hour period.
In the early stages of development 14C from leaves on the main shoot was transported throughout the plant but accumulated in greatest amounts in the meristematic regions. Assimilates produced by the leaves of newly formed tillers were distributed mainly to the meristematic regions of those tillers although labelled carbon was moved in into the rest of the plant. In the later stages of development of each tiller the labelled assimilate was restricted to the tiller itself and to any very young tillers associated with it. After car emergence the pattern of distribution of the labelled carbon from the youngest and second youngest leaves was found to differ. Movement was predominantly towards the ear from the flag leaf and towards the root system from the leaf below the flag.
It was noticed that mature leaves accumulated some labelled material and the possibility that this occurred via the transpiration stream following migration from phloem to xylem, as is the case with some of the case with some of the translocated herbicides, was investigated, but no indication of xylem transference was obtained.
The possible application of these studies to investigations into the relationship between the movement natural assimilates and of translocated herbicides is discussed.  相似文献   

5.
Summary. A method of exposing seedlings of Norway spruce (Picea abies (L.) Karst.) to 14CO2 is described. Within the 1st hr alter 14CO2 exposure, no translocation of the 14C out of the treated branch could be observed. After a 24-hr period, however, the 14C in dormant seedlings had been translocated basipetally to part of the root system only, with no lateral diffusion of the 14C-compounds in the stem. About a week after exposure, both symplastic and apoplastic patterns of translocation had caused a more uniform distribution of 14C. In seedlings at active internode elongation, the translocation patterns were fundamentally identical to those in dormant seedlings, but the active shoot growth had led to a more uniform distribution of the 14C.
Simazine at 20 ppm had apparently stimulated both the photofixation of 14CO2 and the rate of translocation of the 14C-assimilates. At 30 ppm, however, simazine had blocked the translocation of nutrients to the roots. On the other hand, the 14CO2 uptake was not influenced. The simazine incubation had apparently no influence on the synthesis of cationic photosynthate.  相似文献   

6.
Summary. Plants of Potamogeton nodosus, a submersed aquatic, were treated with the di-sodium salt of 7-oxabicyclo(2,2,l)heptane-2,3-dicarboxylic acid-14C (endothal-14C). Gross radioautographs showed that the 14C label moved from mature photosynthesizing leaves and accumulated in the apices and developing secondary plants. Similar results were obtained with mid-leaf, stem (internode) and winter-bud applications. No move-ment of radioactivity occurred following root treatment. The results indicate symplastic translocation of endothal-14C when applied to the leaf, stem or winter bud. It is suggested that endothal can cause the death of plants by direct injury to root tissues subsequent to absorption.
Recherches sur la migration de l'endothal-14C dans le Potamogeton nodosus Poir.  相似文献   

7.
Summary. An autoradiographic study of the distribution of 14C-labelled assimilates, derived from 14CO2 fed to the aerial parts, was made at ten different developmental stages of Oxalis latifolia H.B.K. and Oxalis pes-caprae L. Changing patterns of distribution of labelled assimilates to the underground parts of both species were revealed. These patterns are discussed in the context of the timing of control by defoliation and by foliageapplied herbicides.
Etude autoradiographique de la distribution des métabolites marqués an 14C à différents stades de développement de l'Oxalis latifolia H.B.K. et de l' O. pes-caprae L.  相似文献   

8.
Studies of the absorption and translocation of foliage-applied ring-labelled [14C]asulam [methyl (4-aminobenzenesulphonyl) carbamate] were carried out using glasshouse and field-grown bracken plants. Translocation of 14C from the treated frond was primarily according to a 'source to sink’pattern with intense accumulation of radioactivity in the metabolically active sinks viz. rhizome apices, frond buds, root tips and young frond tissue. In the case of field bracken, translocation and distribution of 14C was extensive in the rhizome system, accumulation occurring in the active as well as dormant buds situated on the non-frond-bearing and storage rhizome branches. Treatment of fully expanded fronds with 100μl of [14C]asulam (1 mg, 1.0–1.5 μCi) as 2 μl droplets resulted in a rapid initial uptake during the first week, followed by progressive entry and distribution with time. Basipetal translocation to the rhizome system was positively correlated with total uptake. High humidity (95%) and high temperature (30°C) stimulated uptake and subsequent basipetal translocation to a considerable degree. Uptake was greater through the stomatal-bearing abaxial than through the adaxial cuticle. Incorporation of a surfactant (Tergitol-7, 0.1%) increased penetration by up to 30%. Uptake declined markedly as the frond aged, while translocation was predominantly acropetal in young treated fronds, becoming exclusively basipetal when the fronds matured. Optimum uptake and maximum distribution of [14C]asulam in the rhizome and its associated buds was achieved when treatments were applied to almost fully expanded fronds. The translocated 14C (asulam and possibly some of its metabolites) showed a considerable degree of persistence in the rhizome system, 8% of the applied activity still remaining in the rhizome 40 weeks after treatment.  相似文献   

9.
Experimental systems were produced from fragments of bracken rhizome and picloram-14C was applied to frond laminae, rhizome apices, frond buds and roots and translocation assessed 7 days after treatment. The isotope was readily taken up by all organs and freely translocated to associated fractions of the rhizome except in the case of laminae from which distribution was very poor. Accumulation of activity in the roots was considerable following treatment of the frond buds but was limited in the frond buds. Poor translocation of herbicide from treated frond laminae is considered a possible explanation of poor control in the field when bracken is sprayed in July. Lapplication du picioram e14C à la fougère Des systèmes expérimentaux ont étéétablis en utilisant des fragments de rhizome de fougére et du piciorame 14C a été appliqué sur les limbes des frondes, les apex des rhizomes, les bourgeons des frondes et les racines. La migration fut évaluée sept jours aprés le traitement. L'isotope a été facilement absorbé par tous les organes et librement transporté aux fractions correspondantes du rhizome, sauf dans le cas des limbes è partir desquels la distribution a été très faible. L'accumulation de ractivité dans les racines a été considérable è la suite du traitement des bourgeons de frondes mais a été Iimitée dans les bourgeons de frondes. Une faible migration de I'herbicide depuis les limbes des frondes est considérée comme une explication possible du désherbage mediocre auchamp, lorsque la fougère est traitée en juillet. Die Anwendung von PicIoram-14C zu Adlerfarn Aus Rhizomstücken von Adlerfarn wurden Versuchsp-flanzen gezogen und auf die Wedelspreiten, Rhizomapices, Wedelknospen und Wurzein- Picloram-14C appliziert. Die Transiokation des Herbizids wurde eine Woche nach der Behandlung gemessen. Das lsotop wurde von alien Pflan-zenorganen schnell aufgenommen und in die dem Rhizom benachbarten Pflanzenteile transloziert; bei Behandlung der Wedelspreiten war jedoch nur eine geringe Verteilung fest-zustellen. Nach Behandlung der Wedelknospen war in den Wurzein eine beträchtliche, aber in den Wedelknospen nur eine geringe Aktivitätsanreicherung feststellbar. Für den schwachen Bekämpfungserfolg des Adierfarns im Freiland bei Spritzungen im Juli, wird die geringe Transiokation des Herbizids aus den Wedelspreiten als mögliche Erklärung angesehen.  相似文献   

10.
A. J. SANAD 《Weed Research》1971,11(4):215-223
Studies of the uptake and translocation of 14C-labelled 2, 4-D, MCPA and aminotriazole in Agrostemma githago L. and Tussilago farfara L. clarified the behaviour of the herbicides in both species. In A. githago, MCPA was more freely mobile than 2,4-D after application to the leaf; it was distributed in the plant more rapidly and in greater quantity. Similarly, following root uptake MCPA was transported in the shoot in greater amounts than was 2,4-D. There is a clear relationship between the susceptibility of A. githago to MCPA and the mobility of the herbicide in the plant. In T. farfara, 2,4-D and aminotriazole applied to the leaves were equally well absorbed and relatively rapidly translocated. During the period up to 72 h the amounts of herbicide in the plant increased to similar levels; after that, 14C activity in plants treated with 2,4-D fell slightly whereas there was further accumulation of aminotriazole. Following uptake through the roots, translocation and accumulation in the leaves were considerably greater with aminotriazole than with 2,4-D. The lack of accumulation of 2,4-D could be a factor in the resistance of T. farfara to this herbicidie. Recherches sur l'absorption et la migration d'herbicides marqués au 14 C dans Agrostemma githago L. et Tussilago farfara L.  相似文献   

11.
This study was conducted to determine the effects of three organosilicone-based and six conventional organic adjuvants on the absorption and translocation of 14C-glyphosate in guineagrass and redroot pigweed. The organosilicone adjuvants produced rapid absorption of the 14C-glyphosate into the redroot pigweed leaves, reaching maximum absorption within 0.5–1.0 h after application. The conventional adjuvants produced slower absorption of the 14C-glyphosate, as the maximum absorption was not achieved until at least 24 h after application in redroot pigweed, remaining similar until 72 h. In guineagrass, the maximum absorption of the glyphosate was earlier than 24 h with the organosilicone-based adjuvants, compared with longer times for the conventional adjuvants. The organosilicone-based adjuvants also increased the glyphosate translocation in redroot pigweed, but not in guineagrass. Organosilicone adjuvants have the potential to provide greater rainfastness to glyphosate on redroot pigweed and, to a lesser extent, on guineagrass.  相似文献   

12.
Rooted cuttings of Pyrus melanocarpa (Michx.) Willd. had a 3.5-fold greater tolerance to soil-applied hexazinone than those of Rubus hispidus L. in a greenhouse trial. R. hispidus accumulated four times more 14C-label in the foliage following root uptake of 14C-hexazinone than P. melano-carpa . Here, the greater uptake and susceptibility of R. hispidus was related to its greater root:fol-iage (weight) ratio compared to the tolerant P. melanocarpa . However, in whole plant metabolism studies employing younger plants there were no differences in 14C accumulation in the leaves. Here 14Chexazinone was converted to a number of hydroxylated and/or demethylated triazinone metabolites in both species following vacuum infiltration into leaf disks and root sections, or following root uptake in whole plants. A major difference in metabolism between the two species was the greater formation of the mono demethylated metabolite, B, [3-cyclohexyl-6-methylamino-l-methyl-l,3,5-triazine-2,4-dione] in P. melanocarpa which may also contribute to its greater tolerance. A loss of total 14Clabel from all plant parts with time may reflect cleavage of the 14C-ring-labelled herbicide.  相似文献   

13.
Summary. Absorption and translocation of 14C-labelled herbicides applied in culture solutions lo the roots and in concentrated drops to mature leaves of 'Thompson Seedless' (Sultanina) cuttings were studied. Neither 2,4-D nor paraquat, and only traces of 2,4,5-T translocated from the roots to the shoot. Dicamba, amitrole, diuron, monuron, simazine and atrazine readily moved from the roots to the shoots. The substituted ureas and s -triazines were mobile only in the xylem or cell walls (apoplast), while dicamba and amitrole were mobile in the apoplast and symplast (living protoplasm). Both 2,4-D and 2,4,5-T moved almost entirely in the symplast. Paraquat moved only slightly in either apoplast or symplast. Stability of the herbicides in the plants varied, as judged by the detection of label in ethanol-insoluble products. Dicamba was considerably more stable than 2,4,5-T which, in turn, was more stable than 2,4-D. Diuron was more stable than either atrazine or simazine. Amitrole largely was converted into ethanol-insoluble products in 30 days. Paraquat was not extractable with ethanol.
Absorption et migration d'herbicides chez une vigne: Thompson Seedless (Sultanina), Vitis vinifera L.  相似文献   

14.
Absorption and translocation of 14C-glyphosate was evaluated under controlled conditions in peas ( Pisum sativum L.) and broad beans ( Vicia faba L.) parasitised or not by crenate broomrape Orobanche crenata Forsk.). Absorption increased with time up to 12 days after treatment, and reached about 50% of the 14C-glyphosate applied. Three days after treatment 70–85% of the total herbicide absorbed had been translocated out of the treated leaflet. There was no consistent differ ence in absorption and translocation between infected and non-infected plants 12 days after treatment. The 14C-glyphosate concentration in the root system increased with time in broad beans. In peas it remained more or less constant from 3 days after treatment. Generally, pods were stronger sinks for glyphosate than other parts of the plant. Herbicide accumulation in broomrape increased with its growth stage, and the parasite was a much stronger sink for glyphosate than the legume root system.  相似文献   

15.
Summary. Autoradiographs and liquid scintillation counts of cucumber ( Cucumis sativus L. cv. Ashley) seedlings indicated rapid absorption and translocation of radioactivity from fluorodifen-1'-14C ( p -nitrophenyl-α,α,α-trifluoro-2-nitro- p -tolyl ether labelled with 14C in the 1 position of the p -nitrophenyl ring). After 24 h in 1 mg/1 of fluoro-difen-1'-14C, 25% of the absorbed radioactivity was translocated from the roots.
Cucumber seedlings degraded fluorodifen-1'-14C via a pathway similar to that reported for peanut seedlings but at a slower rate. The major degradation products were p -nitrophenol and an unidentified polar compound (Unknown I). More rapid absorption and translocation coupled with slower degradation by cucumber as compared with peanut seedlings are factors which may contribute to the susceptibility of cucumber to fluorodifen.  相似文献   

16.
Summary. Picloram is decarboxylated to a negligible extent in roots of skeleton weed: applied to leaves it kills roots better than 2,4-D; it penetrates leaves better than 2,4-D and disrupts the translocation system more, does not leak considerably into the soil from roots by the time of appropriate harvests, yet is not as well translocated as 2,4-D. Under such conditions its ability to kill roots to a greater depth is attributable to its greater intrinsic toxicity.
There is some evidence that under certain field conditions useful movement or uptake of picloram may occur via the soil.
Recherches sur Chondrilla juncea L . avee le plclorame et le 2,4-D.  相似文献   

17.
Summary Plants of Paspalum distichum L. with two stolons growing over water were treated separately with the sodium salt of 2,2-dichloropropionic acid-36C1 (dalapon), 3-amino-1,2,4-triazole-14C (amitrole) and 1,1'-dimethyl-4,4'-dipyridylium dichloride-14C (paraquat) at four different situations on one stolon. Autoradiographs showed that both the amitrole and dalapon label accumulated in the apices of the treated stolons and in some of the young shoots growing from the base of the plant. The 14C from the paraquat label showed predominantly xylem translocating with no apical accumulation and little basal translocation. The results indicate that young basal shoots rely to some extent on assimilates from the mature stolons but that there is little transport between mature stolons.
La migration et la distribution de trois Herbicides marqués dans Paspalum distichum L.  相似文献   

18.
There is limited information on the effect of amitrole and 2,4-D ester applied preplant and pre-emergence in soybean (G lycine max L.) in Ontario, Canada. Six field trials were conducted over a 2 year period (2004 to 2005) at three Ontario locations to evaluate the response of soybean to amitrole or 2,4-D ester applied at 14 days preplant (DPP), 7 DPP, 1 day after planting (DAP), and 7 DAP. The application of amitrole resulted in as much as 5.8, 3.9, 1.7, and 1% visible crop injury in soybean at 7, 14, 28, and 56 days after emergence (DAE), respectively. There was no visible injury in soybean with any amitrole treatment at 56 DAE, except for amitrole applied at 7 DAP, which caused 1% visible injury in soybean at 2310 g ha−1. The application of the 2,4-D ester caused ≤8.3, 9.7, 4.6, and 1.3% visible injury in soybean at 7, 14, 28, and 56 DAE, respectively. The visible injury decreased over time. There was no visible injury in soybean with any of the 2,4-D ester treatments at 56 DAE, except for the 2,4-D ester treatment applied at 7 DAP, which caused 1% visible injury at 1155 g ha−1 and 1.3% visible injury at 2310 g ha−1. Soybean generally responded similarly to amitrole and 2,4-D ester when applied at 14 and 7 DPP; however, soybean was more tolerant to amitrole compared to 2,4-D ester when applied at 1 or 7 DAP. The application of amitrole and 2,4-D ester resulted in no biomass or yield reduction in soybean compared to the weed-free, untreated control at all doses and application timings evaluated. Soybean is tolerant to the preplant and pre-emergence application of amitrole or 2,4-D ester at the doses evaluated.  相似文献   

19.
Summary. Tracer studies using single drops of solutions containing 3–amino-1,2,4–triazole-5–14C (aminotriazole-14C) or 2,2–dichloropropionic acid-2–14C (datapon-14C) revealed that in couch plants (Agropyron repens (L.) Beauv.) growing under field conditions in the autumn and at the stage where the aerial shoots were 40–50 cm long, both compounds moved in both symplast and apoplast. Dalapon was less mobile in the symplast than aminotriazole and only negligible amounts of dalapon were translocated to the rhizomes. The nodes of the treated shoots appeared to act as barriers to translocation, a phenomenon more pronounced for dalapon than for aminotriazole.
Application to a basal green leaf led to a more uniform distribution of the compounds within plants and rhizomes than when the application was made to the youngest fully-expanded leaf.
In couch plants with aerial shoots 10–15 cm long treated in the stubble, the distribution of both aminotriazole and dalapon was mainly restricted to the treated shoots. Even 15 days after application only trace amounts of radioactivity could be found in the rhizomes and untreated shoots.  相似文献   

20.
Summary. The herbicides studied were 2,4-D, 2,4-DB, dicamba and orthoarsenic acid. Herbicide content in the roots was taken as an overall measure of penetration into and absorption by the leaves, and of translocation to the roots.
A significantly greater 2,4-D content resulted from foliar application at pH 3–5 than at higher values, though at pH 8–5 the inclusion of triethanolamine significantly increased the 2,4-D content. No evidence was obtained that a greater 2,4-D content should result from foliage applications of 2,4-DB than from 2,4-D. Dicamba gave a greater herbicide content than 2,4-D when applied at high concentration at 20° C but not at 25° C, probably because of less injury at the lower temperature.
Concentrations of Tween 20 up to 2% had no deleterious effect on the 2,4-D content; on the other hand 2,4-D content was lowered by 0–25% or more of cetyltrimethyl-ammonium bromide. Poor wetting is not the cause of the variable herbicide contents sometimes obtained.
Orthoarsenic acid, which has given better control of the weed than 2,4-D, was very poorly translocated; its effectiveness is due to its high intrinsic toxicity.
Etudes sur la teneur en herbicide des racines de Chondrilla juncea L. á la suite d'applications sur les feuilles  相似文献   

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