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1.
Azamal Husen 《New Forests》2008,36(1):13-27
A study was conducted on the twelve clones of shisham (Dalbergia sissoo Roxb.). These clones were obtained from India and Nepal. Single-node leafy cuttings were prepared from the vegetative multiplication
garden to examine the clonal variation, effect of IBA treatment on rooting response and associated metabolic changes during
adventitious root formation. A remarkable and significant variation was observed due to treatment of 2,000 ppm IBA in the
rooting parameters. Clonal variations were also significant for root and shoot growth while length of root was insignificant.
Among the twelve clones studied; C3 (Tulsipur, Gonda, Uttar Pradesh, India) and C4 (Laxmipur, Gonda, Uttar Pradesh, India)
clone cuttings have given the highest rooting response. Interaction (clone × IBA) was significant only for production of number
of roots per cutting. Periodic sampling for clone C3 was performed at 0, 7, 14, 21, 28 and 35 days to examine the contents
of total soluble sugars, starch, protein and peroxidase (PER) activity in the rooting zone of cuttings (∼0.5 cm) during adventitious
root primordium development. A significant increase in all the metabolic activities was noted due to IBA. Total soluble sugars
and starch contents of cuttings decreased with the passage of time. Protein content and PER-activity started to increase in
the early stage and reached the highest level on day 21, followed by a decline at the 35th day of sampling. These trends were
common for both IBA treated and untreated cuttings. Protein content and PER-activity remained higher in the rooting zone of
IBA treated cuttings. Overall these findings suggested that exogenous application of IBA may have activated carbohydrate metabolism
for release of energy, while protein and PER-activity were necessary for cell division and differentiation during adventitious
root primordium initiation and development in the rooting zone of cuttings. 相似文献
2.
Shoot anatomical features viz., number of vessel elements, width and length of vessel and fibre elements were studied in relation
to juvenility/maturity of teak (Tectona grandis Linn. f.). The findings show significant differences in these features, which indicates that variation in shoot anatomy can
be used as a reliable indicator of juvenility/maturity in teak. The effect of age of donor plants and application of auxins
on adventitious root formation in single noded leafy stem cuttings of teak was also investigated. Aging of donor trees suppressed
rooting and sprouting of cuttings, but increased callus formation at the base of cuttings. Per cent rooting and per cent sprouting
in cuttings decreased as age of donor plants increased from 2-months to 15-years and up to 30-years. IBA treatment promoted
per cent rooting as well as per cent sprouting, NAA promoted only rooting and it suppressed sprouting. The effect of auxin
treatment on rooting and sprouting of teak stem cuttings varied with the age of donor plants. Auxin and its concentration,
which suppressed rooting and sprouting in cuttings of younger donor plants, actually promoted rooting when cuttings were taken
from more mature donor plants. It appears that the auxin requirement for causing and promoting rooting in teak cuttings increased
with increasing age of donor plants. The higher auxin requirement for causing and promoting rooting in cuttings of older trees
may be due to a decrease in the content on endogenous auxins or decreased sensitivity of aging tissues to rooting promoters
and /or accumulation of inhibitory substances which inhibit rooting. 相似文献
3.
The main objective of our clonal propagation program is the production of high quality timber and faster tree growth. Teak
(Tectona grandis Linn. f.) is an important plantation species in the tropics but the demand of teak timber is increasing globally. Teak planting
stock is still produced from seeds of unselected elite genotypes which may lead to poor stand establishment. The number of
seeds per fruit and their germination ability are also variable and limited by several factors. Therefore an alternative method
i.e., clonal propagation is required for mass multiplication of elite trees. It is an established fact that the clonal propagation
or adventitious root formation in cuttings is markedly affected by several external and internal factors. Considering these,
an experiment was conducted on leafy soft wood shoot cuttings of 3-year-old hedged teak stock plants grown in a hedge garden
to study the effects of branch position and auxin treatment on adventitious root formation. A factorial completely randomized
design was used for experimentation. Coppice shoots were collected from basal, middle and upper positions of the hedge garden;
and mono-nodal leafy cuttings were prepared. These cuttings were treated with different concentrations of IBA and NAA; and
were cultured in a mist chamber for rooting where the relative humidity was maintained at 85 ± 2% with maximum and minimum
day-night temperature at 32 ± 1°C and 26 ± 1°C respectively. After 45 days the cuttings were removed from the rooting medium
and observations were made. It was observed that the rooting parameters viz., percent rooting and percent sprouting, mean
number of leaves, mean number of shoots and length, and mean number of roots and length per cutting were significantly influenced
by the branch position and auxin treatment. Treatment with 4000 ppm IBA increased percent rooting and percent sprouting whereas
NAA suppressed it. Treatment with 4000 ppm IBA also increased the mean number of leaves and shoots, and the length, and the
mean number of roots per cutting. The highest mean number of roots and length were observed in cuttings taken from the branch
emerging at the upper position followed by branches of middle and lower position. Cuttings of branches at a middle position
showed the highest values of percent rooting, percent sprouting, mean number of leaves and shoots and mean shoot length per
cutting. Furthermore, the interactive effect of branch position and auxin treatment on rooting and sprouting response was
also found to be significant. Overall, the findings of this investigation suggest that middle branch position in hedge plants
and 4000 ppm IBA can be made to induce maximum rooting and to produce high quality planting stock material for clonal forestry
program of teak. 相似文献
4.
【目的】研究不同生长调节剂处理对金叶银杏硬枝扦插生根率和成活率的影响,探讨金叶银杏的生根机制,为金叶银杏苗木扦插快繁技术体系的建立提供技术支持和理论指导。【方法】通过NAA和IBA处理金叶银杏硬枝插穗,检测它们对插穗的生根状态、生根指标及IBA对扦插过程中插穗皮部可溶性糖、抗氧化酶、激素含量的影响,探究金叶银杏硬枝扦插繁殖技术和生根机理。【结果】1)金叶银杏插穗愈伤组织诱导期和形成期、不定根发生期和形成期分别在插后的20~26、26~42、42~53、53~79 d。2)800 mg·L^-1 IBA和400 mg·L^-1 NAA+400 mg·L^-1IBA(混合)处理的插穗愈伤组织和不定根出现期及生根率与根数都较其他相应处理的效果好,其中生根率分别达64.4%和51.1%;不定根数分别达8.6条和9.3条。3)插穗生根过程中(500 mg·L^-1 IBA处理)基部韧皮部的SOD、PPO活性在插后40、60 d达到高峰,均比对照提前20 d达到峰值;插穗的POD活性出现上升-下降-升高的趋势,插穗可溶性糖也出现升高-下降-升高的趋势,但时间点不一致;而淀粉的含量出现下降-上升的趋势。4)生根期间插穗基部韧皮部的IAA含量出现了2个峰值和一个谷值;而ZR含量在第40天达到峰值后下降;GA含量先下降后上升;ABA含量在插后的前期高后期低,且值都低于同期对照。IAA/ABA比值在插后的第40天(愈伤组织形成期)达到谷底,后又上升(不定根形成期)。IAA/GA在插后的40~60 d内快速上升,80 d后又开始大幅下降;IAA/ZR值在扦插愈伤组织形成期(40 d)呈下降趋势,到60 d时(不定根发生期)又急剧上升到峰值。【结论】高活性的SOD和POD有利于插穗初期愈伤组织的形成(40 d),高活性的POD与PPO有利于不定根的形成(60 d)。较高的IAA含量是保证愈伤组织形成(20~40 d)的必要条件,不定根形成期(60 d)需要较高的IAA/ZR、IAA/ABA、IAA/GA比值。本研究为金叶银杏扦插繁殖技术和生根机理的研究及金叶银杏苗木的快速繁殖生产提供技术支持。 相似文献
5.
Variable adventitious rhizogenesis of juvenile shoot cuttings was observed in seven half-sib families (TP-1, BAN-1, AN-3,
JR-3, SL-16, UM-3, and UM-5) of Albizia procera Benth. Treatment with IBA invariably promoted sprouting by 8%, adventitious rooting by 218%, root number by 869%, and root
length by 36.4% compared with the control. Interaction between families and IBA treatment significantly enhanced sprouting
and root length in BAN-1, JR-3, UM-3, and UM-5, and root number in all families, but had a significant suppressive effect
on sprouting in AN-3. Treatment with IBA increased family heritability (h
f
2) 1.38-fold for sprouting, 2.86-fold for adventitious rooting, and 10.86-fold for root number, but reduced family heritability
by a factor of 8.25 for root length. Thus, direct genetic effects seem to have strongly affected sprouting and adventitious
rooting, with IBA playing an auxiliary role, but had little effect on root number and length, for which IBA had a dominant
regulatory role. 相似文献
6.
不同生长素处理对桉树无性系插条氧化酶活性影响的比较研究 总被引：23，自引：2，他引：23
尾叶桉MLA无性系 (简称MLA)是扦插难生根无性系 ,尾叶桉U6 无性系 (简称U6 )和刚果 12号桉W5无性系 (简称W5)是相对易生根无性系。分别用吲哚乙酸 (IAA)、吲哚丁酸 (IBA)、萘乙酸 (NAA)处理MLA、U6 和W5插条后 ,各无性系插条体内的过氧化物酶 (POD)、多酚氧化酶 (PPO)、吲哚乙酸氧化酶 (IAAO)活性呈现规律性的升高。在生根过程的不同时期 ,各酶的活性差异极显著 ,表明桉树插条的不定根发生和发展与POD、PPO、IAAO活性有密切联系。而IAA、IBA、NAA对不同无性系和不同的氧化酶活性的促进效果也有显著差异。讨论了生长素促进桉树插条氧化酶活性和不定根发生与发展的机理 相似文献
7.
几种柏科树种嫩枝扦插的研究 总被引：10，自引：2，他引：10
木本植物的插条繁殖至今仍是园林工作者获得良种无性系和培育苗木的重要途径。柏科树种多是珍贵的园林树种,它们的嫩枝插穗生根难易差别很大。本研究探讨了生根难易的生理原因,为提高扦插成活率提供理论依据。 相似文献
8.
骆琴娅 《中南林业科技大学学报(自然科学版)》1991,(2)
用IBA500ppm和1000ppm酒精溶液处理乌桕嫩枝扦插条,能促进插条生根及生长。用500 ppm和1000ppm PP333溶液处理插条,扦插后的前20天表现出对生根有不同程度的抑制作用。但40天时,500 ppm处理的生根率明显增高,1000ppm处理的也出现了愈伤组织,并表明其对根的粗度生长具有促进作用。 相似文献
9.
本试验以中国石竹为插穗,珍珠岩＋蛭石（1：1）为基质,分别采用吲哚乙酸（IAA）、吲哚丁酸（IBA）及萘乙酸（NAA）与两者相混的生根剂处理插穗,研究了不同生根剂对中国石竹扦插生根的影响。结果表明：用IBA＋NAA1000mg／L处理,可使插穗幼根提前形成,可促进根系生长旺盛,提高根系活力,但对可溶性糖含量无明显促进作用。 相似文献
10.
‘Zhongqiusucui’ jujube secondary shoots were treated with 3-indolebutyric acid (IBA) at three concentrations, 500, 1000 and 1500 mg/L. Results show that IBA could significantly enhance rooting and root characteristics of cuttings and were best with IBA at 1500 mg/L. In the rooting process, the formation of adventitious roots was related to the consumption and accumulation of nutrients (soluble sugars and proteins) and the changes in endogenous hormones in phloem, leaf tips and leaf bases. The rooting of cuttings had a positive correlation with the consumption of soluble sugars during the period of callus formation and with the accumulation of soluble sugars during adventitious root formation and growth. Rooting was positively related to the breakdown of soluble proteins in the phloem when the callus formed, and had a positive correlation with its accumulation during adventitious root formation and growth. Leaf tips and leaf bases showed a reverse trend in changes of soluble protein. However, together with the phloem, leaf tips and leaf bases regulated and controlled the formation and development of adventitious roots. The main activities of soluble proteins exist in the leaf tips as this was the main source of soluble proteins. The relation between rooting and IAA (indole-3-acetic acid) content in phloem was positive and thus a high concentration of IAA could benefit the induction and formation of adventitious roots. However, rooting was negatively related with ABA (abscisic acid) and GA (gibberellic acid) and a high concentration of both could inhibit the induction and formation of adventitious roots. Rooting had a positive correlation with phloem IAA/ABA ratios, and higher ratios could improve rooting. Low concentrations of ZR (zeatin riboside) triggered the induction of adventitious roots, while higher concentrations promoted root growth. Endogenous hormones in leaf tips and bases had an impact on rooting. The activities of endogenous hormones mainly existed in leaf tips because they play a major role in the production and consumption of IAA and its ABA content increased during rooting. The ZR in leaf tips influenced the rooting of cuttings, especially in the callus formation and rooting stage. Leaf tips were the main source of GA. 相似文献
11.
A study to determine the best vegetative propagation protocol was carried out for Dalbergia melanoxylon. Natural regeneration of D. melanoxylon is limited due to poor seed viability and germination. The effects of age of donor plant, indole-3-butyric acid (IBA) treatment
and cutting position on rooting of stem cuttings were investigated. The effect of age of donor plant was significant (P < 0.001) only for percent rooting, position effect was significant for all studied parameters, and IBA treatment at 300 ppm
was insignificant only for percent callusing. Interactive effects of cutting position × age of donor and cutting position × IBA
treatment were significant for percent rooting and root number per cuttings while interactive effect of age × IBA was significant
only for percent rooting. Observed difference in rooting performance could be due to uneven distribution of root promoting
and inhibiting substances with respect to age of donor plant and IBA which intensified root formation. Cuttings from juvenile
donor plant performed better in all rooting parameters than mature donor plant. The highest percentages in rooting were (71.11%)
and (24.42%) for cuttings from juvenile and mature donor plants, respectively. IBA treated cuttings produced higher percentage
rooting, number of roots and root length than untreated cuttings which revealed a strong IBA influence on rooting ability
of stem cuttings in D. melanoxylon. It was concluded that stem cuttings should be taken from juvenile donor plants and basal cutting position should be used
to produce high quality planting stock material for the afforestation and conservation programme of D. melanoxylon. 相似文献
12.
不同植物生长调节剂对美人梅硬枝扦插生根的影响 总被引：3，自引：0，他引：3
不同组合和质量浓度植物生长调节剂处理美人梅硬枝插穗试验结果表明：（1）不同植物生长调节剂及质量浓度促根效果不同：NAA、IAA和IBA都表现出低质量浓度促进生根,高质量浓度抑制生根的现象;同时低质量浓度的生长调节剂能使愈伤组织形成提前10～20 d,从而促进提前生根。NAA250 mg/L（浸泡30 min）处理调节生根率最好,其次是IAA500 mg/L（浸泡30 min）的处理,分别达56.67%,54.44%;在对平均单株生根数量上却以IAA500mg/L（浸泡30 min）处理效果最好,平均达11.48条。（2）NAA 250 mg/L不同浸泡时间对美人梅扦插生根效果不同：在供试质量浓度范围内,随着处理时间的增加,生根率、单株平均生根数量均增加,以NAA250 mg/L处理60 min促进生根效果最好,平均生根率达71.11%,平均生根数达10.68条。 相似文献
13.
Adventitious rooting in shoot cuttings of neem (Azadirachta indica) and karanj (Pongamia pinnata) was investigated in different seasons. The influence of auxins and B-vitamins (neem only) was evaluated. Maximum rhizogenesis coincided with the emergence of new sprouts i.e. February (neem) and March (karanj). Considering both rooting percentage and root biomass, IBA was the most effective auxin tested. Compared to auxin free controls, IBA (1000 ppm in neem and 800 ppm in karanj) significantly increased adventitious rooting percentage (80 to 100 % respectively), the numbers of roots and root biomass for both species. In neem, B-vitamin treatment yielded 40–50% rooting for cuttings. This was significantly greater than rooting for controls but equivalent to IAA treatments. In neem, some of the cuttings treated with 2000–3000 ppm IBA had well developed root systems without any sprouts. In karanj, all auxin treatments promoted sprouting. The level of total soluble sugars in the rooting zone of neem and karanj was higher in non-rooted than rooted cuttings. 相似文献
14.
以蝶花荚蒾（Viburnum hanceanum）1 a 生枝条作为插穗,采用IBA、NAA、ABT1#、根森、
根动力、核能素及其不同质量体积浓度进行处理,清水处理作为对照,研究不同生长调节剂种类及其质
量体积浓度对蝶花荚蒾扦插繁殖生根率和根系生长的影响。结果表明：不同生长调节剂对蝶花荚蒾扦插
生根有显著影响,扦插后插穗不定根数、生根率和根鲜质量均在不同处理间达到极显著差异,其中,以
IBA200 mg/L+NAA200 mg/L 处理效果最好,其平均生根率达到88.67%,平均根鲜质量1.14 g/ 株、平均
不定根数6.80 条/ 株。 相似文献
15.
Applying auxins to freshly cut basal ends of leafy single-node cuttings of Triplochiton scleroxylon, an important timber tree of West Africa, considerably enhanced rooting percentages. The optimal concentrations in 10 μl MeOH droplets of a 50 : 50 mixture of α-naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA) ranged from 8–40 μg according to clone. Larger amounts, which tended to increase numbers of roots per rooted cutting, inhibited root production in some clones. When applied singly, IBA was more effective than NAA in most clones.Leafless cuttings rarely rooted even when treated with auxins. Rooting occurred following the retention of 5 cm2 of leaf lamina, and was maximal, as judged by rooting percentages, with 50 cm2. The inability of leafless cuttings to root was associated with the rapid depletion of all carbohydrates in stem tissues, while those in unshaded leafy cuttings tended to increase.Percentages of cuttings rooting increased when temperatures of propagating-beds were raised above 20°C, and 28°C was optimal for most clones, especially if treated with auxins. Cutting survival declined above 28°C, although up to 38°C surviving cuttings rooted equally well. 相似文献
16.
Kiran Kaul 《New Forests》2008,36(3):217-224
The present study investigates optimal conditions for the vegetative propagation of Himalayan yew Taxus wallichiana Zucc., an important medicinal tree, during spring. Effect of four treatments: (a) sex of donor plant (male and female), (b)
age/type of shoot (1, 2, 3 year old, long and dwarf shoots), (c) auxin treatment (IBA and NAA at 0, 0.5, 1.25, 5.0 & 50.0 mM)
and (d) rooting environment (raised beds/polythene bags) on percentage rooting in stem cuttings was studied. Randomized complete
block (RBD) designs were used for experimentation. Rooting ability of cuttings was significantly influenced by all these treatments.
The overall rooting response was higher in long shoot cuttings taken from female tree. Age of shoot also influenced the rooting
response and was highest in 1 year old long shoot cuttings of female tree. Exogenous application of auxin, α-naphthalene acetic
acid (NAA) and indole-3 butyric acid (IBA), had significant positive effect on the percentage rooting. IBA significantly enhanced
the rooting percentage in 1 year old long and dwarf shoots at lower doses and 2 and 3 year old long shoots from female tree
at higher doses. Maximum percent rooting (90% ± 2.8) was obtained with interactive effect of 0.5 mM, NAA (22 h) × 1 year old
long shoot from female tree; followed by the interactive effect of 50 mM IBA (5 s) × 3 year old long shoot from female tree
(83% ± 4.1). Cuttings planted in soil: sand medium in polythene bags showed earlier rooting response (12 weeks) than cuttings
planted in raised nursery beds (24 weeks). Overall, the findings of this study suggest that 0.5 mM IBA treatment is suitable
for enhancing adventitious rooting in 1 year old long and dwarf shoots of male and female trees. IBA at higher doses is suitable
for enhancing the rooting percentage of 2 and 3 year old long shoots from female tree. This study provides a significant lead
towards the development of a simple and inexpensive technique for large scale propagation, aforestation of elite genotypes
and raising of bush type plantation under ex-situ conditions. 相似文献
17.
Tests were conducted to assess rootability of red alder softwood cuttings from shoots of young trees and epicormic sprouts of mature individuals. Ortets were 3 to 34 years old, and treatments with and without indole-3-butyric acid (IBA) were compared. The extent of rooting and root vigor on the cuttings varied greatly among ortets and treatments. Among nonterminal cuttings, the best overall rooting success, with and without IBA, was obtained with cuttings of the youngest ortet. Average rooting of terminal and nonterminal cuttings of one clone were 100% and 64%, respectively. Although cuttings of some trees rooted well without IBA, the IBA treatment appeared to be generally beneficial. For all nonterminal cuttings of all clones, best results (80.0%/x) were obtained with a 10-sec dip in 2,000 or 4,000 ppm IBA. Vigorous planting stock was obtained from the rooted cuttings of all clones. Using epicormic sprouts from mature trees of proven performance will presumably allow propagation of superior trees and establishment of new plantations with improved stock. 相似文献
18.
激素处理南方红豆杉扦插生根的研究 总被引：10，自引：5，他引：5
应用吲哚乙酸、吲哚丁酸、奈乙酸三种不同的生长素对插条进行处理,探讨它们在不同扦插基质上的生根率。基质配比采用（４,４）单形重心设计,结果重心设计,结果是：三种生长素中以吲哚乙酸对扦插生根的影响最好,基质以４５％黄心土和５５％珍珠岩配比效果最好。插穗浸泡时间不同对扦插生根率也产生了显著影响。 相似文献
19.
为了解油茶(Camellia oleifera)扦插生根过程中营养物质含量的动态变化及其与插穗生根的关系,以清水为对照,用IBA、ABT6、NAA生根粉处理插穗,并对油茶嫩枝扦插生根过程中部分营养指标的含量进行了测定.结果表明:外源生长激素提高了插穗生根率,改善了生根性状,其中以IBA处理为最优;处理组与对照插穗的可溶性总糖质量分数、淀粉质量分数随时间变化趋势一致,均呈"下降—升高—下降"趋势,处理组出现的峰值均比对照早且高;总氮质量比变化呈"升高—下降—升高"趋势,处理组全氮含量在根的表达期均低于对照,但生根率均高于对照; C/N值呈现"下降—升高—下降"变化趋势,处理组C/N值变化曲线的峰值显著高于对照,C/N值越高的处理,其生根率越高. 相似文献
20.
【目的】为了解生长素诱导杜梨不定根发生效应,明确不定根诱导过程与氧化酶活性间的关系,从而为杜梨等难生根植物的生根研究提供理论指导和技术借鉴。【方法】以连续继代培养的杜梨不定芽为材料,调查了不同种类(NAA、IBA和IAA)和浓度(0.1、0.5、1.0、2.0 mg·L-1)的生长素对不定芽生根的影响情况,分别在生根诱导0、4、8、12、16 d后观察不定根发育的内外组织形态,测定并分析相关酶活性的变化情况。【结果】外源生长素均能促进不定芽不定根的发生,在相同种类的生长素处理下,浓度分别为0.1和2.0 mg·L-1处理的各项生根指标均显著低于浓度分别为0.5和1.0 mg·L-1处理的。以NAA诱导产生的愈伤组织较大且松软易脱落,不利于其后期的移栽成活;以IBA处理的生根率显著高于NAA和IAA处理的,其中以浓度为0.5 mg·L-1的IBA处理的各项生根指标均最优。对其组织形态的观察结果表明,杜梨不定根发生类型属于诱生根原基,经IBA处理4 d后,其茎基部表皮颜色变红,内部根原基发生于韧皮部和维管束形成层交界处,诱导8 d后茎段外部出现了愈伤组织且组织内部根原基已形成,诱导12 d后不定根突破表皮向外生长。在杜梨不定芽生根过程中,SOD和PPO活性在诱导的0~4 d内均快速上升,在诱导的4~12 d内均下降,而在诱导的12~16 d内均呈现缓慢上升的变化趋势;IAAO活性总体呈现先下降后上升的趋势,且每个时期的活性均显著高于对照组;POD活性呈现出上升→下降→上升→下降的变化趋势,2个峰值分别出现在诱导4 和12 d之时。【结论】0.5 mg·L-1的IBA可有效促进连续继代培养的杜梨不定芽的生根,其不定根的形成过程与氧化酶有密切的关系。 相似文献