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1.
The objective of this study was to eval- uate the effects of inorganic phosphorus source and phytase addition on performance, nutrient digestibility and bone mineralization in broiler chickens. In Exp. 1,150 two-day old, male broiler chicks were fed a corn-soybean meal basal diet supplemented with phos- phorus provided by dicalcium phosphate, tricalcium phosphate or defluorinated rock phosphate. Five cages containing 10 birds were allotted to each of the three treatments. In Exp. 2,120 three-day old, male broiler chicks were fed the basal diet from Exp. 1 supplemen- ted with 0,250,500 ,or 1,000 P-＇rU phytase per kg of diet. Six cages containing five chicks were allotted to each of the four treatments. In Exp. 1, there was no difference in weight gain, feed intake or feed conver- sion as a result of feeding the different sources of in- organic phosphorus. The digestibility of phosphorus was significantly lower （P =0.01 ） for chicks fed di- ets supplemented with tricalcium phosphate than for chicks fed the other two diets. However, despite the lower digestibility, serum phosphorus levels did not differ among the three treatments. For Exp. 2, feedconversion showed a linear improvement （P = 0.03 ） with increasing levels of phytase inclusion （ days 0 to 33 ）. Phytase supplementation resulted in linear increa- ses in the digestibility of dry matter （P = 0.02 ）, crude protein （ P --- 0.04 ） and energy （ P 〈 0.01 ）. Chicks fed 1,000 FTU/kg phytase had significantly higher bone calcium （ P = 0.05 ） and bone breaking strength （P = 0.04 ） than chicks fed the basal diet on day 33. In conclusion, the results of the current study indicated that the performance of birds fed diets sup- plemented with dicalcium phosphate, tricalcium phos- phate or defluorinated phosphate was similar and therefore production costs could be lowered by choo- sing the cheapest inorganic phosphorus source when formulating diets for poultry. When diets were formu- lated to meet dietary phosphorus requirements, the growth of broilers was not enhanced with phytase sup- plementation. However, increases in feed conversion and bone breaking strength and its potential to impact culling and mortality in broiler operations may be suf- ficient justification for the routine inclusion of phytase in diets fed to broilers.  相似文献   

2.
1. Two experiments were conducted to investigate the effect of dietary enzyme supplementation of a low-protein diet on performance, nitrogen excretion, fat deposition, hepatic lipogenic and lipolytic enzyme activity in 7-21- (Experiment 1) and 21-42-d-old (Experiment 2) male broiler chicks. 2. Chicks were given diets containing 210 g (Experiment 1) or 170 g (Experiment 2) crude protein (CP)/kg (Control), amino acid-fortified diets 190 g (Experiment 1) and 150 g (Experiment 2) CP/kg (Low-protein), and a low-protein diet supplemented with 1000 U/kg of cellulase. 3. In Experiment 1, growth performance and abdominal fat deposition were not affected by dietary treatments, and birds given low-protein diets excreted less nitrogen. The activities of hepatic lipogenic and lipolytic enzymes were not different among the three dietary groups. 4. In Experiment 2, the dietary treatment did not affect growth performance or abdominal fat weight. Nitrogen excretion was significantly lower in chicks given the 150 g/kg CP diet than those on the 170 g/kg CP diet; however, nitrogen retention was no different among the treatments. Dietary CP and enzyme supplementation did not significantly affect hepatic enzyme activities. 5. These results suggest that CP content in the broiler diet can be reduced by 20 g/kg without lowering performance by the supplementation of crystalline amino acids, and can reduce nitrogen excretion by about 25%. Cellulase supplementation of a low-CP diet slightly lowered abdominal fat deposition; however, it did not significantly affect hepatic lipogenic and lipolytic enzyme activity.  相似文献   

3.
The objective of this study was to evaluate the effects of inorganic phosphorus source and phytase addition on performance, nutrient digestibility and bone mineralization in broiler chickens. In Exp. 1, 150 two-day old, male broiler chicks were fed a corn-soybean meal basal diet supplemented with phosphorus provided by dicalcium phosphate, tricalcium phosphate or defluorinated rock phosphate. Five cages containing 10 birds were allotted to each of the three treatments. In Exp. 2, 120 three-day old, male broiler chicks were fed the basal diet from Exp. 1 supplemented with 0, 250, 500, or 1,000 FTU phytase per kg of diet. Six cages containing five chicks were allotted to each of the four treatments. In Exp. 1, there was no difference in weight gain, feed intake or feed conversion as a result of feeding the different sources of inorganic phosphorus. The digestibility of phosphorus was significantly lower (P = 0.01) for chicks fed diets supplemented with tricalcium phosphate than for chicks fed the other two diets.  However, despite the lower digestibility, serum phosphorus levels did not differ among the three treatments. For Exp. 2, feed conversion showed a linear improvement (P = 0.03) with increasing levels of phytase inclusion (days 0 to 33).  Phytase supplementation resulted in linear increases in the digestibility of dry matter (P = 0.02), crude protein (P = 0.04) and energy (P < 0.01).  Chicks fed 1,000 FTU/kg phytase had significantly higher bone calcium (P = 0.05) and bone breaking strength (P = 0.04) than chicks fed the basal diet on day 33. In conclusion, the results of the current study indicated that the performance of birds fed diets supplemented with dicalcium phosphate, tricalcium phosphate or defluorinated phosphate was similar and therefore production costs could be lowered by choosing the cheapest inorganic phosphorus source when formulating diets for poultry. When diets were formulated to meet dietary phosphorus requirements, the growth of broilers was not enhanced with phytase supplementation.  However, increases in feed conversion and bone breaking strength and its potential to impact culling and mortality in broiler operations may be sufficient justification for the routine inclusion of phytase in diets fed to broilers.  相似文献   

4.
试验研究了豆粕日粮和含5%～8%棉粕的日粮中添加α-半乳糖苷酶对肉仔鸡生长性能的影响及可能机制。试验Ⅰ为肉仔鸡饲养试验,选用1日龄AA商品代肉仔鸡公雏672只,随机分为4个处理,每个处理12个重复,每个重复14只鸡,试验期42d。结果表明:在棉粕和豆粕日粮中添加α-半乳糖苷酶对42日龄肉仔鸡体增重分别提高了4.35%和3.49%。试验Ⅱ为排空强饲代谢试验。结果显示:豆粕日粮中添加α-半乳糖苷酶增加蛋公鸡色氨酸真消化率(P<0.001),对其他氨基酸消化率无影响。添加α-半乳糖苷酶的豆粕的,真代谢能值与不添加酶的相比,提高了4.69%(0.51MJ/kg)。本研究表明,在玉米-豆粕型日粮中添加α-半乳糖苷酶,提高了色氨酸真消化率和真代谢能,有利于提高肉仔鸡生长性能。  相似文献   

5.
Young chicks exhibited a 61% reduction in weight gain when a corn-soybean meal diet was supplemented with 15 mg/kg Se provided as Na selenite. The same level of Se provided as selenomethionine depressed weight gain by 32%. Supplementing the high selenite diet with isoarsenous (14 mg/kg As) additions of As2O5, As2O3, phenylarsonic acid, phenylarsine oxide and roxarsone ameliorated the Se-induced growth depression: As2O5 almost totally restored growth rate; As2O3, phenylarsonic acid and phenylarsine oxide gave intermediate responses; and roxarsone gave only a small ameliorative growth response. Arsanilic acid was without effect in stimulating growth rate of selenite-intoxicated chicks. Dietary addition of .4% L-cysteine produced a growth response in selenite intoxicated chicks that was somewhat greater than that obtained with roxarsone; administering both roxarsone and cysteine corrected growth better than either compound given singly. Both roxarsone and As2O5 also effectively ameliorated the Se-toxicity growth depression caused by selenomethionine (15 mg Se/kg) supplementation, but cysteine showed no efficacy against morbidity caused by this form of Se. Liver Se concentration was elevated 10-fold by selenite and 25-fold by selenomethionine supplementation. The arsenic compounds had varying effects on liver Se, whereas cysteine tended to increase Se concentration. These findings suggest that both inorganic and organic arsenicals as well as cysteine ameliorate selenium toxicity by different mechanisms.  相似文献   

6.
1. A total of 192 male broilers (Cobb 500) were used in a growth and digestibility assay, involving a 2 x 2 factorial arrangement of treatments, to assess the effects of an enzyme cocktail of xylanase, amylase and protease in maize-based diets. 2. The following two diets were formulated: a positive control diet containing adequate nutrient concentrations for broiler starters as per breeder recommendations and a negative control diet to contain approximately 0.63 MJ/kg apparent metabolisable energy (AME) and 3% amino acids less than the positive control. 3. A further two dietary treatments were developed by supplementing each control diet with an enzyme product containing xylanase, amylase and protease. 4. Birds fed on the negative control diet had poorer weight gain and feed efficiency than those given the positive control diet. There was no effect of diet or enzyme on feed intake. The digestibility of nitrogen, calcium, phosphorus and most amino acids were unaffected by dietary nutrient density. 5. Supplementation of both the positive and negative control diets with the enzyme improved weight gain and feed efficiency compared with the unsupplemented diets. In the case of the negative control, supplemental enzyme improved performance to that of the unsupplemented positive control diet. There was no interaction between diet and enzyme for either weight gain or FCR, suggesting similar beneficial responses regardless of the nutrient density of the diet. 6. In both diets, enzyme supplementation improved AME by an average of 3% (0.35 MJ/kg DM) and nitrogen retention by an average of 11.7% (26 g/kg DM intake vs 29 g/kg DM intake). There was no interaction between diet and enzyme for AME or nitrogen retention. 7. Ileal digestibilities of calcium and phosphorus were not influenced by supplemental enzyme, whereas the digestibility of nitrogen and most amino acids was improved by enzyme addition compared with the unsupplemented control diets. There was no interaction between diet and enzyme for the ileal digestibility of nitrogen and amino acids. 8. These data demonstrate that it is possible to improve the nutritional value of a maize/soy-based diet for broiler starters through the use of exogenous enzymes. The nutrient density of the diet does not appear markedly to influence the response to enzyme, offering flexibility in the use of enzymes for maize-based diets. 9. It is concluded that the energy and amino acid values of maize-based diets for broilers can be enhanced by supplementation with an enzyme cocktail of xylanase, amylase and protease, offering potential economic benefits to producers.  相似文献   

7.
Four trials investigated the effect of high levels of three phytase enzymes on P and protein utilization in chicks. The three phytases were derived from Aspergillus (Fungal Phytase 1), Peniophora (Fungal Phytase 2), and E. coli. Within each assay, 8-d-old male chicks were given ad libitum access to their experimental diet for 10 to 14 d. For Trials 1, 2, and 3, the basal diet was a corn-soybean meal diet deficient in P that was analyzed to contain 23% CP and 0.38% total P (0.10% estimated available P, as-fed basis). Phytase supplementation levels were based on the assessment of phytase premix activity (i.e., P release from Na phytate at pH 5.5 and 37 degrees C). In Trial 1, supplementation of inorganic P from KH2PO4 (0 to 0.20%) resulted in a quadratic (P < 0.05) response in weight gain, gain:feed, and tibia ash concentration but a linear (P < 0.01) increase in tibia ash weight. Tibia ash was higher (P < 0.01) for chicks fed E. coli phytase than for those fed Fungal Phytase 1 at 500, 1,000, and 5,000 phytase units (FTU)/kg, but did not differ between these two phytases at 10,000 FTU/kg. In Trial 2, E. coli phytase supplementation at 1,000 FTU/kg maximized growth and bone responses, whereas addition of either of the two fungal phytases resulted in increasing responses up to 5,000 and 10,000 FTU/kg. Dietary addition of Fungal Phytase 2 resulted in the poorest (P < 0.01) responses among the three phytases. Escherichia coli phytase supplementation at 10,000 FTU/kg in Trial 3 resulted in tibia ash (millligrams) responses that were greater (P < 0.05) than those resulting from either 0.35% inorganic P supplementation or 10,000 FTU/kg of Fungal Phytase 1 or 2. Trial 4 showed that E. coli phytase supplementation at either 500 or 10,000 FTU/ kg did not improve protein efficiency ratio (gain per unit of protein intake) of chicks fed low-protein soybean meal or corn gluten meal diets that were first-limiting in either methionine or lysine, respectively. These results demonstrate that high dietary levels of efficacious phytase enzymes can release most of the P from phytate, but they do not improve protein utilization.  相似文献   

8.
为研究不同锰源与锰水平对肉仔鸡的生产性能与组织矿物元素沉积的影响,试验采用2×5完全随机试验设计,在玉米—豆粕型基础日粮(锰含量为37.66mg/kg)中添加0、45、90、130、180mg/kg(5个锰添加水平)的复合氨基酸锰和硫酸锰(2种锰源),构成10个日粮处理组,将540只1日龄健康艾维茵肉仔鸡随机分为10个处理组,开展肉仔鸡饲养试验(共42d)。于35日龄时测定其生产性能指标,计算料肉比;于42日龄时测定其胫骨、肝脏、肾脏组织中铜、铁、锌浓度以及胫骨灰分、钙及磷含量。结果表明,在上述日粮中,锰添加量在45~130mg/kg时,无论是添加复合氨基酸锰组还是添加硫酸锰组35日龄肉仔鸡体重和采食量较未添加组差异显著(P≤0.05),经分析,锰水平对其平均体重及采食量有极显著影响(P<0.01);不同锰源的35日龄肉仔鸡体重、采食量、料肉比差异不显著(P >0.05)。42日龄肉仔鸡胫骨、肝脏、肾脏组织中铜、锌、铁含量及胫骨灰分、钙磷含量未受锰源和锰水平及两者互作的显著影响(P >0.05)。由以上结果可以得出,锰水平对肉仔鸡生长性能有极显著影响(P<0.01);锰源对肉仔鸡生产性能无显著影响(P >0.05);锰源和锰水平及两者互作对肉仔鸡组织矿物元素沉积无显著影响(P >0.05)。  相似文献   

9.
The overall objective of the studies reported here was to evaluate the growth and nutrient utilization responses of pigs to dietary supplementation of phytate- or nonstarch polysaccharide-degrading enzymes. In Exp. 1, growth performance and nutrient digestibility responses of forty-eight 10-kg pigs to dietary supplementation of phytase or a cocktail of xylanase, amylase, and protease (XAP) alone or in combination were evaluated. The growth response of one hundred fifty 23-kg pigs to dietary supplementation of phytase or xylanase individually or in combination was studied in Exp. 2 in a 6-wk growth trial, whereas Exp. 3 investigated the nutrient digestibility and nutrient retention responses of thirty 24-kg pigs to dietary supplementation of the same enzymes used in Exp. 2. In Exp. 1, the pigs were used in a 28-d feeding trial. They were blocked by BW and sex and allocated to 6 dietary treatments. The treatments were a positive control (PC) diet; a negative control (NC) diet marginally deficient in P and DE; NC with phytase added at 500 or 1,000 phytase units (FTU)/kg; NC with xylanase at 2,500 units (U)/kg, amylase at 400 U/kg, and protease at 4,000 U/kg; and NC with a combination of phytase added at 500 FTU/kg and XAP as above. In Exp. 2 and 3, the 5 dietary treatments were positive control (PC), negative control (NC), NC plus 500 FTU of phytase/kg, NC plus 4,000 U of xylanase/kg, and NC plus phytase and xylanase. In Exp. 1, low levels of nonphytate P and DE in the NC diet depressed (P < 0.05) ADG of the pigs by 16%, but phytase linearly increased (P < 0.05) ADG by up to 24% compared with NC. The cocktail of XAP alone had no effect on ADG of pigs, but the combination of XAP and phytase increased (P < 0.05) ADG by 17% compared with the NC treatment. There was a linear increase (P < 0.01) in Ca and P digestibility in response to phytase. In Exp. 2, ADG was 7% greater in PC than NC (P < 0.05); there were no effects of enzyme addition on any response. In Exp. 3, addition of phytase alone or in combination with xylanase improved (P < 0.05) P digestibility. Phosphorus excretion was greatest (P < 0.01) in the PC and lowest (P < 0.05) in the diet with the combination of phytase and xylanase. The combination of phytase and xylanase improved P retention (P < 0.01) above the NC diet to a level similar to the PC diet. In conclusion, a combination of phytase and carbohydrases improved ADG in 10-kg but not 23-kg pigs, but was efficient in improving P digestibility in pigs of all ages.  相似文献   

10.
Several bioassays were conducted with young chicks and pigs fed phosphorus (P)-deficient corn-soybean meal diets. With diets for chicks containing .62% Ca and .42% P (.10% available P), graded doses of a citric acid + sodium citrate (1:1, wt:wt) mixture (0, 1, 2, 4, or 6% of diet) resulted in linear (P < .01) increases in both weight gain and tibia ash. Relative to chicks fed no citric acid, tibia ash (%) and weight gain (g/d) were increased by 43 and 22%, respectively, in chicks fed 6% citric acid. Additional chick trials showed that 6% citric acid alone or sodium citrate alone was as efficacious as the citric acid + sodium citrate mixture and that 1,450 U/kg of phytase produced a positive response in bone ash and weight gain in chicks fed a diet containing 6% citrate. Varying the Ca:available P ratio with and without citrate supplementation indicated that citric acid primarily affected phytate-P utilization, not Ca, in chicks. Moreover, chicks did not respond to citrate supplementation when fed a P-deficient (.13% available P), phytate-free casein-dextrose diet. Young pigs averaging 10 to 11 kg also were used to evaluate citric acid efficacy in two experiments. A P-deficient corn-soybean meal basal diet was used to construct five treatment diets that contained 1) no additive, 2) 3% citric acid, 3) 6% citric acid, 4) 1,450 U/kg phytase, and 5) 6% citric acid + 1,450 U/kg phytase. Phytase supplementation increased (P < .01) weight gain, gain:feed, and metatarsal ash, whereas citric acid addition increased only gain:feed (P < .05) and metatarsal ash (P < .08). A subsequent 22-d pig experiment was conducted to evaluate the effect of lower levels of citric acid (0, 1, 2, or 3%) or 1,450 U/kg phytase addition to a P-deficient corn-soybean meal diet. Phytase supplementation improved (P < .01) all criteria measured. Weight gain and gain:feed data suggested a response to citric acid addition, but this was not supported by fibula ash results (P > .10). The positive responses to phytase were much greater than those to citric acid in both pig experiments. Thus, dietary citric acid effectively improved phytate P utilization in chicks but had a much smaller effect in pigs.  相似文献   

11.
1. Non-genetically modified (non-GM) phytase product derived from Aspergillus niger possesses various side active enzymes including α -amylase, protease, cellulase and hemicellulase. In contrast, the product of genetically modified (GM) phytase product has much less side active enzyme since the capacity of phytase production is reinforced by gene modification. In the present study we have tried to determine whether the difference of side enzyme activity of phytase product affects growth performances and nutritive value in chicks; in addition we tried to characterise the physiological change induced by the difference of side active enzymes. 2. Single Comb White Leghorn male chicks at 7 d of age were fed on experimental barley-based diets for 10 d. The feeding trial was of a factorial design (3 × 2 × 2), having three types of dietary phytase products (control, non-GM or GM phytase products derived from A. niger at 1000 U/kg diet), two levels of dietary available P supplement (0 or 6 g/kg diet) and two levels of dietary protein (CP 180 or 120 g/kg). 3. The non-GM phytase product caused a 6% increase in final body weight and feed efficiency com6 pared with the control and the GM phytase product without interacting with dietary protein and available P level. However, in birds given available P-free diet, both non-GM and GM phytase products induced a 20% increase in plasma P concentration, suggesting no difference in phytase activity between the non-GM and GM phytase products. 4. The balance study showed that the metabolisable energy of the non-GM phytase product (15.6 ± 0.05 kJ/g diet) was significantly higher among the treatments (control, 15.1 ± 0.05; GM phytase product 15.3 ± 0.07). The non-GM phytase product also increased the rate of food passage through the crop, and caused a drastic reduction in intestinal weight, perhaps as a consequence of digestion of non-starch polysaccharides. 5. We conclude that the side active enzymes in non-GM phytase product improve growth performance and nutritive value of the diet in chicks. However, the efficacy of phytase activity should not be different between non-GM and GM phytase products.  相似文献   

12.
The experiment was conducted to assess the effects of dietary supplementation of Cu on the growth performance, digestive enzymes, tissue minerals and absorptive transporters in small intestinal mucosa of weanling pigs. One hundred crossbred pigs weaned at 28 ± 2 d of age were assigned randomly to one of the following diets with 5 replicates: corn-soybean basal diet with 10, 100, 175, 250 mg/kg of Cu as CuSO4•5H2O. The results showed that 250 mg/kg Cu had a positive effect (P < 0.05) on average daily gain, daily feed intake and ratio of gain/feed. Compared to 10 mg/kg Cu, higher Cu had significant effect on the apparent digestibility of protein and fat (P < 0.05). The supplementing of Cu improved amylase and lipase activity in jejunum content and lipase in pancreas (P < 0.05) and had no effect on intestinal morphology. The liver Cu elevated approximately 4-fold in pigs fed diet with 250 mg/kg Cu compared with pigs fed diet with 10 mg/kg Cu, no increases were observed in pigs receiving the lower level of Cu (100 and 175 mg/kg). Both Fe and Zn contents in kidney and liver were not affected by Cu supplementation. There was no positive effect (P > 0.05) of Cu supplementation on PepT1 (peptide transporter 1) and SGLT1 (sodium/glucose cotransporter) mRNA abundance in intestinal mucosa. However, higher supplementing level (250 mg/kg) significantly elevated the DMT1 (divalent metal transporter) mRNA abundance in duodenum mucosa. These results suggested that dietary supplementation with 250 mg/kg Cu could improve growth performance, nutrient digestibility and intestinal enzyme activities of weanling pigs.  相似文献   

13.
本试验旨在探讨单宁酸对生长猪胃、小肠仿生消化中消化酶活性及玉米-豆粕型饲粮干物质和粗蛋白消化率的影响，为评价单宁酸的生物学效应提供参考。试验一采用单因素完全随机设计，考察在无饲粮下2种单宁酸对猪模拟胃液、模拟小肠液消化酶活性的影响。设5个处理，单宁酸添加量分别为0 mg （胃液体积为20 mL，小肠液体积为22 mL）；单宁酸1，10 mg；单宁酸1，20 mg；单宁酸2，10 mg；单宁酸2，20 mg。测定各处理的胃蛋白酶、淀粉酶、胰蛋白酶和糜蛋白酶的活性。试验二考察玉米-豆粕型饲粮添加单宁酸对猪仿生消化中胃、小肠阶段消化酶活性及养分消化率的影响。采用单因素完全随机设计，设5个处理，单宁酸在饲粮中的含量分别为0 mg·（2 g）-1；单宁酸1，10 mg·（2 g）-1；单宁酸1，20 mg·（2 g）-1；单宁酸2，10 mg·（2 g）-1；单宁酸2，20 mg·（2 g）-1。测定仿生消化中胃阶段0.5和4 h时胃蛋白酶活性，小肠阶段0.5、4和8 h时淀粉酶、胰蛋白酶和糜蛋白酶活性及生长猪胃-小肠仿生消化测定饲粮的干物质和粗蛋白消化率。结果表明：1）无饲粮的情况下，和空白对照组相比，2种单宁酸对模拟胃液中胃蛋白酶活性无显著影响（P>0.05），单宁酸1比单宁酸2更高地降低了模拟小肠液中淀粉酶、胰蛋白酶和糜蛋白酶的活性（P<0.05）。2）在饲粮进行仿生消化的胃消化0.5～4 h内，除4 h时10 mg·（2 g）-1添加量外，添加单宁酸1时胃蛋白酶的活性均显著高于添加单宁酸2时的相应值（P<0.05），除单宁酸2在消化0.5 h外，2种单宁酸在添加10 mg·（2 g）-1时胃蛋白酶活性均显著高于20 mg·（2 g）-1添加量的相应值（P<0.05）。在小肠仿生消化0.5 h时，饲粮中添加单宁酸1、2的2个水平对消化液中淀粉酶活性无显著影响（P>0.05），但均显著降低了糜蛋白酶的活性（P<0.05）；单宁酸1的消化液中胰蛋白酶活性高于单宁酸2的相应值（P<0.05）。在小肠仿生消化4 h时，除添加水平为20 mg·（2 g）-1时的糜蛋白酶活性外，饲粮中添加单宁酸1消化液中淀粉酶、糜蛋白酶活性高于添加单宁酸2的相应值，而胰蛋白酶活性低于添加单宁酸2的相应值（P<0.05）。单宁酸1、2的两个添加量均降低了胰蛋白酶和糜蛋白酶的活性（P<0.05）。在小肠仿生消化8 h时，饲粮中单宁酸的添加量影响了淀粉酶的活性，但单宁酸1和单宁酸2各两个添加量在淀粉酶的平均活性上无显著差异（P>0.05）。单宁酸1、2的两个添加量均降低了胰蛋白酶、糜蛋白酶的活性（P<0.05）。3）与对照组相比，两种单宁酸在两种添加水平下均显著降低了饲料粗蛋白消化率（P<0.05），且单宁酸2比单宁酸1更多地降低了饲粮粗蛋白的消化率（P<0.05）。综上所述，在有、无饲粮条件下，单宁酸对消化酶活性呈现不一致影响。单宁酸影响饲粮粗蛋白的消化率可能主要与消化液中糜蛋白酶活性降低以及单宁酸与饲粮中的化学成分形成螯合物降低了小肠消化酶的水解效率有关。  相似文献   

14.
The effects of dietary supplementation of zinc (Zn) sources and concentrations were investigated on growth performance, absorption into tissues, fecal excretion, nutrient retention, and intestinal morphology in broilers fed a corn-soybean meal basal diet. A total of 525 one-day-old chicks (Ross 308) were assigned based on body weight to seven dietary treatments. There were five replicate pens for each treatment and 15 broilers per replicate pen. The dietary treatments included a basal diet (control, without supplementing Zn), and basal diet supplemented with Zn, as inorganic zinc sulfate (ZnS; 110 mg/kg); organic Zn-methionine (ZnM; 110 mg/kg); hot-melt extruded (HME) 25 zinc sulfate (27.5 mg/kg); HME50 zinc sulfate (55 mg/kg); HME75 zinc sulfate (82.5 mg/kg); or HME100 zinc sulfate (110 mg/kg) for 35 days in two phases (d 1–21, phase I and d 22–35, phase II). Bodyweight and feed efficiency of broiler chicks fed diets supplemented with increasing dietary concentrations of HME-Zn improved linearly during the study period (P<0.05). Compared to the control treatment, the ZnS, ZnM, and HME diets increased Zn concentrations in the serum and liver. Inorganic ZnS supply resulted in the highest Zn concentration in excreta. Increasing supplemented Zn content in diets as HME linearly increased Zn concentration in the excreta, serum, liver, and tibia. Broiler chicks fed diets supplemented with increasing concentrations of HME increased villus height (VH; linear and quadratic) of the jejunum and VH of the ileum (linear). Increasing concentrations of dietary Zn supplied as HME resulted in linearly enhanced dry matter, gross energy, and nitrogen retention of broilers on day 21. These results suggest that dietary HME-Zn at a lower level (55 ppm) shows the same growth performance as common ZnSO4 at 110 ppm.  相似文献   

15.
试验选用270羽1日龄艾维茵健康肉仔鸡,随机分成3组,研究了玉米-豆粕型日粮条件下添加不同水平的木聚糖酶对肉鸡生产性能和内源酶活性的影响。结果表明,添加25g/t和50g/t的木聚糖酶可提高肉鸡的日增重,降低料重比;提高胃蛋白酶、胰蛋白酶、胰淀粉酶和胰脂肪酶酶活力,同时亦显著提高了肉鸡的生产效益。  相似文献   

16.
Four growth experiments were conducted to assess the effects of organic acid supplementation on performance of starter and finisher pigs. Three 4-wk starter experiments utilized 392 pigs fed simple corn-soybean meal diets. A fourth experiment employed 135 finisher pigs in a 6-wk study. Each of the starter experiments was initiated immediately after weaning; piglets were 30 +/- 3 d of age. In Exp. 1, weanling pigs fed a 19% crude protein, simple corn-soybean meal diet were compared with pigs fed similar diets supplemented with 2% propionic, fumaric, or citric acid. Addition of each acid improved (P less than .07) efficiency of gain, while propionate depressed (P less than .05) feed intake. Additions of 1, 2, 3 or 4% fumarate were made in Exp. 2, resulting in linear daily gain and feed efficiency improvements (P less than .05). In Exp. 3, a possible protein-sparing effect of fumaric acid was investigated. Increasing protein levels from 16 to 20% improved daily gain (P less than .01) and feed efficiency (P less than .0001); fumarate supplementation (2%) increased (P less than .01) gain:feed. However, there was no protein X fumaric acid interaction. In Exp. 4, no treatment effects were noted with performance of finisher pigs fed a 14% crude protein, corn-soybean meal diet was compared with that of pigs fed similar diets supplemented with 1.5 or 3% fumaric acid.  相似文献   

17.
An experiment was conducted with 208 day-old male Cobb feather-sexed chicks to study tissue accumulation of Cu as an estimate of biological availability of inorganic Cu sources for chicks. Chicks were allotted randomly to dietary treatments that included an unsupplemented basal corn-soybean meal diet (11.1 mg/kg Cu, DM basis) or this basal diet supplemented with 150, 300 or 450 mg/kg Cu either as reagent-grade acetate or feed grade oxide, carbonate or sulfate. Chicks were housed in batteries and allowed ad libitum access to feed and tap water for 21 d. Liver Cu was not affected by dietary Cu from the oxide source, but it increased (P less than .001) with increasing dietary Cu from all other sources. Bone Cu was lower (P less than .05) in chicks fed 150 mg/kg Cu compared to other dietary levels of Cu but was not influenced (P greater than .10) by Cu source. Using the slope-ratio technique from regression of log liver Cu on mean daily Cu intake with Cu from acetate set at 100%, the relative biological availability values were estimated to be 88.5 and 54.3% for sulfate and carbonate, respectively, and oxide was essentially unavailable.  相似文献   

18.
有机锌源对肉鸡生长性能和胫骨软骨症的影响   总被引：4，自引：0，他引：4  
采用玉米－豆饼型饲粮研究有机锌源对肉鸡生长性能和胫骨软骨症（ＴＤ）的影响。结果表明,有机锌源与硫酸锌对肉鸡生长和饲料采食量均无显著影响,锌的补加提高了胫骨,中趾骨,胰Ｚｎ含量,有机锌源的补加并不影响骨灰含量,胫骨长度,不诱导肉鸡ＴＤ发病率上升。  相似文献   

19.
1. A total of 2208 broiler chicks were used in two growth experiments (8 treatments and 12 replicate pens in each experiment) to assess the effects of xylanase, amylase, protease and phytase in maize-based diets. 2. A positive control diet was formulated containing adequate nutrient concentrations. A negative control diet was formulated to contain approximately 628 kJ/kg, 0.13%, 0.12% and 1 to 2% less metabolisable energy (ME), phosphorus (P), calcium (Ca) and amino acids, respectively, than the positive control. In addition, two further negative control diets that contained 167 or 334 kJ/kg more ME, respectively, than negative control 1 were formulated. 3. A further 4 dietary treatments were made by supplementing each of the 4 negative control diets with a combination of xylanase, amylase, protease and phytase, resulting in 8 dietary treatments in a 4 by 2 factorial arrangement. 4. The scale of the removal of energy, P, Ca and amino acids from the positive control diet was determined using least square models based on in vivo data for both the xylanase/amylase/protease cocktail and for phytase and it was predicted that performance of birds fed on negative control 1 would be returned by supplemental enzymes to that of those fed on the positive control. 5. In both experiments there was a significantly poorer performance in birds fed on the negative control 1 than in those fed on the positive control. The poorer weight gain and feed conversion ratio could be attributed in part to a reduced intake of digestible energy, P, nitrogen (N) and amino acids associated with birds fed on the negative control diet. 6. Supplementation of the negative control diets with the enzyme combination returned performance to that of the positive control in both experiments. 7. These data indicate that exogenous xylanase, amylase, protease and phytase can be used successfully in a strategically formulated low nutrient density diet to maintain performance to that of birds fed on a nutritionally adequate diet.  相似文献   

20.
Four chick trials and one pig trial were conducted to investigate the phosphorus-releasing efficacy oftwo commercial phytase enzymes (Natuphos and Ronozyme) and an experimental E. coli phytase enzyme (ECP) when added to corn-soybean meal diets containing no supplemental inorganic P (iP). In the 13- or 14-d chick trials, three or four graded levels of iP (0, 0.05,0.10,0.15%) from KH2PO4 were added to the basal diet to construct standard curves from which bioavailable P release could be calculated for the phytase treatments. In all cases, phytase supplementation levels were based on an assessment of phytase premix activity (i.e., P release from Na phytate at pH 5.5). Linear (P < 0.01) responses in tibia ash and weight gain resulted from iP supplementation in all assays. In the first chick trial, supplementation of 500 phytase units (FTU)/kg of ECP resulted in superior (P < 0.01) weight gain and tibia ash values compared with 500 FTU/kg of Natuphos. Results of the second chick trial revealed P-release values of 0.032 and 0.028% for 500 FTU/kg Natuphos and Ronozyme, respectively, and these were lower (P < 0.01) than the 0.125% P-release value for 500 FTU/kg of ECP. Tibia ash responded quadratically (P < 0.05) in response to graded levels of ECP up to 1,500 FTU/kg in the third chick trial. Combining Natuphos with either Ronozyme or ECP in Chick Trial 4 revealed no synergism between phytases with different initiation sites of P removal. The pig trial involved 10 individually fed weanling pigs per diet, and and phytase enzymes were supplemented to provide 400 FTU/kg in diets containing 0.60% Ca. Based on the linear regression of fibula ash on supplemental iP intake (r2 = 0.87), P-release values were 0.081% for Natuphos, 0.043% for Ronozyme, and 0.108% for ECP. These trials revealed an advantage of the E. coli phytase over the commercial phytases in young chicks.  相似文献   

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