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Camptothecine (CPT) and hydroxycamptothecin (HCPT), two kinds of anti-cancer alkaloids, were extracted from Camptotheca acuminata leaves using homogenate extraction technology under different conditions such as the ratio of material to liquid, ethanol
concentration, and homogenate time. The optimum technology parameters for homogenate extraction of CPT and HCPT from C. acuminata leaves were determined as homogenate time at 8 min, ethanol concentration at 55% and the ratio of material to liquid at 1:15
(g:mL). By using the optimized parameters, we obtained 0.639‰ extraction rate for CPT and 0.437‰ for HCPT. The extraction
yields of CPT and HCPT extracted by homogenating technology were higher than those by other extractive methods, such as ultrasonic,
reflux, shaking in water bath. It is concluded that the homogenate extraction technology was an efficient method for extracting
CPT and HCPT from C. acuminata leaves, with characteristics of less extraction time and high yield.
Foundation project: This research was supported by National Key Technology R & D Program (grant no. 2006BAD18B04).
Biography: SHI Wei-guo (1970–), male, Ph. D. candidate in Northeast Forestry University, Harbin 150040, P. R. China.) 相似文献
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优化喜树果中喜树碱和10-羟基喜树碱的匀浆提取工艺 总被引：1，自引：0，他引：1
采用匀浆提取的方法从喜树果中提取喜树碱和10-羟基喜树碱，考察乙醇体积分数、匀浆时间和液料比等因素对提取率和纯度的影响，并通过响应面法进行工艺优化，分别得到以喜树碱、10-羟基喜树碱的提取率和纯度为响应值的回归方程，进而获得最佳工艺条件为乙醇体积分数52．41％；匀浆时间2．93min；液料比11．11（mL：g）。最佳条件下的验证实验表明：喜树碱提取率为0．0923％，浸膏中喜树碱的纯度为1．0633％，与模型值相对偏差分别为0．75％和0．88％；10-羟基喜树碱提取率为0．0094％，浸膏中10-羟基喜树碱的纯度为0．1078％，与模型值相对偏差分别为0．53％和0．78％，说明回归方程可以很好地预测实验结果。 相似文献
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建立了一种简便、快速、准确的测定喜树(Camptotheca acuminata Decne.)种子中喜树碱和10-羟基喜树碱含量的高效液相色谱分析方法;色谱条件为:采用日本KYA HIQ sil C18柱(250 mm×4.6 mm,5 μm),流速为1 mL/min,梯度洗脱程序为:在前 15 min流动相乙腈 -水的体积比由10 %线性增加至40%,在随后的3 min乙腈 -水的体积比线性降至10 %并保持恒定3 min,在21 min时停止该程序.检测器为二极管阵列检测器,喜树碱定量分析波长254 nm,10-羟基喜树碱定量分析波长266 nm,进样量10 μL.样品制备以60 %的乙醇作溶剂,在60 ℃下超声波提取喜树种子50 min.利用以上方法分别测定了喜树种子胚乳、胚轴、子叶和种皮中的喜树碱和10-羟基喜树碱含量,喜树碱的含量是胚乳＞胚轴,子叶＞种皮,10-羟基喜树碱的含量是胚乳＞种皮＞胚轴＞子叶. 相似文献
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匀浆法提取喜树果和喜树叶中喜树碱的研究 总被引：2，自引：0，他引：2
以喜树果和喜树叶为原料,对匀浆法提取喜树碱的工艺进行了研究,并采用HPLC 法进行定量分析.确定了最佳的工艺条件:提取溶剂为体积分数 55 % 的乙醇,匀浆时间为 8 min,料液比为1 ∶ 15(g ∶ mL).在此工艺条件下,喜树果和喜树叶中喜树碱的得率分别为 0.080 1 % 和 0.067 9 %.将该法与超声波提取、回流提取、常温冷浸提取、水浴振荡提取等方法进行了比较.结果表明,匀浆提取具有得率高、时间省等优势,是一种高效提取喜树碱的方法. 相似文献
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为了探讨大叶榉树Zelkova schneideriana叶片黄酮类成分,以1年生大叶榉树叶片为材料,在超声波辅助下,通过单因素和Box-Behnken试验,探究并优化了大叶榉叶片黄酮类乙醇提取方法;采用常规显色法,对大叶榉树叶片中黄酮类物质种类进行初步鉴定。结果表明:(1)优化后的大叶榉树黄酮类提取方法为:乙醇浓度63%、液料比31:1mL·g^-1、提取时间1.7 h,大叶榉树黄酮类提取量为(31.067±0.11)mg·g^-1;(2)初步鉴定发现:大叶榉树叶片中含有黄酮、具有邻二酚羟基结构的黄酮类化合物、具有邻二苯酚羟基或兼有3-OH,4=O或5-OH,4=O的化合物;可能有黄酮醇、二氢黄酮、二氢黄酮醇等;不含查尔酮和橙酮。 相似文献
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《林业研究》2020,31(4)
Camptotheac acuminata Decne is a unique tree species in China with an important secondary metabolite,camptothecin(CPT),used in the treatment of cancer.Nitrogen(N) is an important element that affects plant growth and the accumulation of CPT.Reports on the effect of N on CPT synthesis from a genetic perspective are scarce.To explore the effects of different N sources and levels on CPT synthesis in C.acuminata,two-year-old seedlings were fertilized with different concentrations of pure ammonium sulphate,source of ammonium N(NH_4~+-N),and potassium nitrate for nitrate N(NO_3~--N).Concentrations of 2.5,5,7.5,and10 g pot~(-1) NH_4~+-N and NO_3~--N were used.The results showed that 7.5 g NH_4~+-N and NO_3~--N treatments were best for growth and fresh weight of leaves.Compared with the other treatments,the CPT content,tryptophan synthase and tryptophan decarboxylase activities,and expression of the CaTSB and CaTDCl genes under the 2.5 g NH4+-N and NO_3~--N treatments peaked significantly at 30 days.However,the expression of CaTDC2 surpassed that of the other two genes at 60 days.Therefore,compared with NH4+-N source,the NO_3~--N source was more beneficial for growth,and NO_3~--N was better for CPT yield.Consequently,leaves of C.acuminata treated with 2.5 g NO_3~--N could be harvested after 30 days to obtain maximum CPT content.CaTDC1 is more closely linked to CPT synthesis.The results of this study improved the production of CPT in C.acuminata via fertilization. 相似文献
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Liu Z Carpenter SB Bourgeois WJ Yu Y Constantin RJ Falcon MJ Adams JC 《Tree physiology》1998,18(4):265-270
We investigated variation in concentration of the secondary metabolite, camptothecin (CPT), in relation to leaf, branch and tree age, season, and leaf drying method in Camptotheca acuminata Decaisne saplings. Younger leaves contained higher CPT concentrations than older leaves. Within a branch, there was a linear decline in CPT concentration from leaves at the apex of the branch down to Leaf 7. Comparing leaves of similar age, those from younger trees had higher CPT concentrations than those from older trees. Over the course of the growing season, there was a steady decline of 11% per month in leaf CPT concentration. Branches showed a similar seasonal decline in CPT concentration to leaves; however, the rate of decline was threefold greater in leaves than in branches. Freeze-dried tissues had a 27% higher CPT concentration than oven- or air-dried tissues, suggesting that oven- and air-drying caused degradation of CPT. The decline in CPT concentration with tissue aging may reflect a genetically determined mechanism whereby, in young trees, chemicals serve as a first line of defense against attacks by herbivores and pathogenic microorganisms until other mechanisms are developed and deployed. We hypothesize that chemical defense mechanisms are programmed for early ontogenic stages, whereas they are induced by biotic and abiotic factors during later ontogenic stages. 相似文献
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超声波辅助提取脱脂红松仁中水溶性多糖的研究 总被引：1，自引：0，他引：1
采用超声波辅助提取法对脱脂红松仁中水溶性粗多糖的提取工艺进行了研究.通过正交试验,分别考察了提取温度、液料比、提取时间及醇沉浓度4个因素对红松仁多糖提取率的影响,得出优化的工艺条件：提取温度为70℃,液料比为20：1,提取时间为40 min,醇沉浓度为80%,此条件下多糖的提取率为3.65%,平均含量为45.38%.结果表明,超声波辅助提取的效率和含量均优于传统热水浸提法,且具有提取温度低、时间短及效率高的优势. 相似文献
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微波技术提取刺三加叶中绿原酸的研究 总被引：1，自引：0，他引：1
采用L9（34）正交实脸设计对影响微波法提取刺三加叶片中绿原酸的溶剂浓度、微波功率、提取时间和提取次数四个因素进行了研究,结果表明,提取刺三加叶中绿原酸的最佳条件是A2B2C3D3,即乙醇浓度60%,功率300W,提取时间3min,提取次数3次。影响因子的主次顺序为乙醇浓度〉提取次数〉提取时间〉微波功率。 相似文献
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通过单因素试验研究乙醇浓度、料液比、提取温度、提取时间4个因素对连翘叶中连翘苷得率的影响。在单因素的基础上,采用正交试验法优化提取工艺。结果表明,连翘叶中连翘苷最佳提取条件为：80％乙醇,料液比1：10,60℃超声波提取8min,在此条件下,连翘苷得率为0．3112％。 相似文献
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Naturally occurring camptothecin(CPT) is an important source of chemotherapeutic agents.The extraction from Camptotheca acuminata is still the main approach to obtain CPT compared with total synthesis.In the present study,ultrasound-assisted extractions(UAE) of CPT from C.acuminata seeds with alkaline solutions were investigated and CPT yield were determined by High Performance Liquid Chromatography.The conditions of alkaline species and concentrations,extraction time,extraction temperature and ultrasonic power were optimized.Results show that both Na3PO4 and Na2CO3 solutions gain good extraction yields,whereas Na3PO4 solution has stronger basicity and need higher concentration than Na2CO3 solution does,thus aqueous Na2CO3 is more beneficial for the extraction.The optimal condition was ultrasonically extracted with 0.5% aqueous Na2CO3 at 50°C and ultrasonic power of 400 W for 60 min.Comparing with UAE with ethanol,the extraction with 0.5% Na2CO3 solution achieves higher yield.Moreover,aqueous Na2CO3 as a solvent has various advantages including non-toxicity,inflammable,non-corrosive and low cost,which ensure this UAE method is a superior method with high utilizing prospect. 相似文献