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1.
为探明覆膜对早春东北黑土玉米叶片光合生理特征的影响,在大田条件下,研究了覆膜对不同施肥条件下玉米拔节期叶片光合参数与叶绿素荧光参数的影响.结果表明:覆膜能够显著增加不同施肥条件下玉米拔节期叶片光合速率、蒸腾速率、气孔导度和水分利用率,而对其他光合参数如细胞间隙CO2浓度和气孔限制值影响不显著.就施肥条件而言,覆膜对N、P处理玉米拔节期叶片光合参数的影响明显强于其他施肥处理.覆膜能够增加施P和PK处理玉米拔节期叶片Fm、Fv/Fm和Fv/Fo值,而显著降低了施NPK处理玉米拔节期叶片相应的荧光参数,覆膜对其他施肥处理玉米拔节期叶片叶绿素荧光参数的影响不显著.  相似文献   

2.
This study aims to explain the effects of silicon (Si) foliar application on gas exchange characteristics, photosynthetic pigments, membrane stability and leaf relative water content of different wheat cultivars in the field under drought stress conditions. The experiment was arranged as a split-split plot based on randomized complete block design with three replications. Irrigation regime (100%, 60%, and 40% F.C.), silicon (control and Si application) and wheat cultivars (Shiraz, Marvdasht, Chamran, and Sirvan) were considered as main, sub and sub-sub plots, respectively. This study was carried out at the Research Farm of the Collage of Agriculture, Shiraz University, Iran, during 2012–2013 growing season. The results showed that foliar application of silicon increased the leaf relative water content, photosynthesis pigments (chlorophyll a, b and total chl and carotenoids), chlorophyll stability index (CSI) and membrane stability index (MSI) in all wheat cultivars, especially in Sirvan and Chamran (drought tolerant cultivars), under both stress and non-stress conditions. However, more improvement was observed under drought stress as compared to the non-stress condition. In contrast, these parameters decreased under drought stress. Si significantly decreased electrolyte leakage in all four cultivars under drought stress conditions. Furthermore, the intercellular carbon dioxide (CO2) concentration (Ci) increased under drought stress. Si application decreased Ci especially under drought stress conditions. Net photosynthesis rate (A), transpiration rate (E) and stomatal conductance (gs) were significantly decreased under drought conditions. Under drought, Si applied plants showed significantly higher leaf photosynthesis rate, transpiration rate, and stomatal conductance. Intrinsic water use efficiency (WUEi) and carboxylation efficiency (CE) decreased in all cultivars under drought stress. However, the silicon-applied plants had greater WUEi and CE under drought stress. The stomatal limitation was found to be higher in stressed plants compared to the control. Exogenously applied silicon also decreased stomatal limitation. Overall, application of Si was found beneficial for improving drought tolerance of wheat plants.  相似文献   

3.
不同水分和施氮量对催吐萝芙木光合特性和生长的影响   总被引：3，自引：0，他引：3  
在西双版纳干季, 田间试验探讨了水分胁迫下施氮量[不施氮、低量施氮(1.25 g·株-1)、高量施氮(3.75 g·株-1)]对两年生催吐萝芙木(Rauvolfia vomitoria Afzel.)生长和光合的影响。结果表明: 水分胁迫显著降低了催吐萝芙木叶片相对含水量(LRWC), 但LRWC仍在85%以上, 属于低度水分胁迫, 其最大净光合速率、气孔导度、比叶面积、茎重比以及株高、基径和生物量的相对生长速率均较无水分胁迫时低。水分胁迫下, 低量施氮可使最大净光合速率、蒸腾速率、根重比升高, 使株高、基径和生物量的相对生长速率增加, 从而明显增加最终根产量; 而高量施氮则会增加幼树对干旱的敏感性, 加重干旱对催吐萝芙木光合和生长的抑制作用。水分与施氮量交互作用对催吐萝芙木叶片相对含水量、最大净光合速率、水分利用效率、比叶面积和根重比的影响显著, 表明施氮量对其影响视水分胁迫状况而不同。因此, 为获得催吐萝芙木最大根产量, 应在干季收获期前少量施用氮肥。  相似文献   

4.
This study investigated the effects of foliar application of normal and nano-sized zinc oxide on the response of sunflower cultivars to salinity. Treatments included five cultivars (‘Alstar’, ‘Olsion’, ‘Yourflor’, ‘Hysun36’ and ‘Hysun33’), two salinity levels [0 and 100 mM sodium chloride (NaCl)], and three levels of fertilizer application. Fertilizer treatments were the foliar application of normal and nano-sized zinc oxide (ZnO). Foliar application of ZnO in either forms increased leaf area, shoot dry weight, net carbon dioxide (CO2) assimilation rate (A), sub-stomatal CO2 concentration (Ci), chlorophyll content, Fv/Fm, and Zn content and decreased Na content in leaves. The extent of increase in chlorophyll content, Fv/Fm and shoot weight was greater as nano-sized ZnO was applied to the normal form. The results show that the nano-sized particles of ZnO compared to normal form has greater effect on biomass production of sunflower plants.  相似文献   

5.
The present experiment comprised seven wheat cultivars, two drought levels (0 and 17% PEG-8000) and four replicates. The seeds of six wheat cultivars (Al-lugaimi, Bonus, Kronos, Yecora-rojo, Irena and Sama) were supplied by the King Saud University, Riyaz, Saudi Arabia, whereas S-24 was obtained from the Department of Botany, University of Agriculture, Faisalabad. The seeds were allowed to germinate and grow for 20 days in medium having full-strength Hoagland's nutrient solution or Hoagland's solution with 17% PEG-8000. For the appraisal of drought tolerance, various physiological traits such as gas-exchange attributes (A, E, Ci, gs , and A/E), leaf water relations (ψw, ψs and ψp) and the activities of key antioxidant enzymes (SOD, POD and CAT) were determined. On the basis of biomass and gas-exchange attributes (A, E, and gs ), cultivars Al-lugaimi and Sama were found to be drought tolerant, cultivars Yecora-rojo and Irena moderately drought tolerant, and cultivars S-24, Bonus and Kronos drought sensitive. However, plant osmotic adjustment and the activities of potential antioxidant enzymes (SOD, POD and CAT) were not found to be associated with drought tolerance of the different wheat cultivars.  相似文献   

6.
为了比较不同烤烟品种的苗期耐旱性差异,选用河南烟区主栽烤烟品种‘豫烟6号’、‘豫烟10号’、‘豫烟12号’和‘中烟100’为供试材料,利用浓度为15%的聚乙二醇(PEG-6000)模拟中度干旱环境,研究不同烤烟品种幼苗生物量、根系形态、叶片气孔特征、叶绿体超微结构和光合参数等指标对干旱胁迫响应机制的差异。结果表明:(1)干旱刺激了幼苗根系生长,抑制了地上种幼苗根冠比均显著提高;‘豫烟6号’和‘豫烟12号’幼苗根系生物量、总根长、根系表面积和根系体积均显著增加,但根系平均直径与对照无显著性差异;而‘豫烟10号’和‘中烟100’根系形态指标增加幅度较小,仅有‘豫烟10号’根系表面积显著增加,而根系平均直径均显著下降。(2)干旱引起‘中烟100’叶片气孔总面积比对照显著增加,‘豫烟12号’仅有气孔长度比其对照增加显著。(3)干旱处理后,‘豫烟6号’和‘豫烟12号’叶绿体整体结构变化不大,而‘豫烟10号’和‘中烟100’中叶肉细胞叶绿体被膜分解,与细胞壁分离;其中‘中烟100’叶绿体平均长度、长宽比和面积均显著降低。(4)与对照相比,干旱组叶片光合作用被抑制,其中‘豫烟10号’和‘中烟100’叶片净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)均显著下降,而胞间CO2浓度(Ci)显著上升,说明干旱胁迫下烤烟光合速率下降是非气孔因素所致。(5)干旱胁迫后‘豫烟6号’和‘豫烟12号’叶片叶绿素总量显著增加,而‘豫烟10号’和‘中烟100’却呈下降趋势。结果表明,‘豫烟6号’和‘豫烟12号’是耐旱型品种,而‘豫烟10号’和‘中烟100’抗旱性较差,抗旱能力排序为‘豫烟6号’‘豫烟12号’‘豫烟10号’‘中烟100’。  相似文献   

7.
The effect of nitrogen (30 and 120 mg N per cuvette) on photosynthetic rate of four cultivars of triticale (‘Bolero’, ‘Grado’, ‘Largo’, and ‘Lasko’) grown 14 days in phytotron was strongly modified by water content (75, 45 and 35% of full water capacity). For plants grown under 35% of full water capacity, it was higher when they were grown under 30 than under 120 mg N/cuvette (9.88 and 8.76 μmol CO2 m?2 s?1, respectively) but for plants grown under 45 and 75% of full water capacity there were not significant differences. Transpiration, stomatal conductance, photosynthetic water use efficiency, and internal water use efficiency were not influenced by nitrogen doses independently of water content. Photosynthetic rate, transpiration, stomatal conductance, photosynthetic water use efficiency, and dry matter of studied cultivars of triticale grown under 45 and 35% of full water capacity and both nitrogen doses were lower than for plants grown under 75% of full water capacity. With lowering of water content stomatal conductance was decreasing similarly as photosynthetic rate e.g. for plants grown under 35% of full water capacity as compared with those grown under 75% of full water capacity average stomatal conductance decreased from 0.209 to 0.138 mol H2O m?2 s?1 and photosynthetic rate from 13.69 to 9.32 μmol CO2 m?2 s?1 and as a result there were not significant differences in internal water use efficiency for all studied combinations (67.09 μmol CO2 mol?1 H2O) which shows that stomatal factors were mainly responsible for changes of photosynthetic rate. With lowering of water content from 75 to 35% of full water capacity the decrease of photosynthetic rate and stomatal conductance was much higher than the decreases of transpiration (from 3.57 to 3.02 mmol H2O m?2 s?1) what shows not direct dependence of transpiration on stomatal conductance (water use efficiency decreased from 3.87 to 3.10 μmol CO2 mmol?1 H2O). The effect of nitrogen on dry matter production was strongly modified by water availability e.g. for plants grown under 35% of full water capacity, dry matter was similarly independent of nitrogen dose but for plants grown under 45 and 75% of full water capacity dry matter was significantly higher than when they were grown under 120 (79.05 and 86.75 mg, respectively) or with 30 mg N/cuvette (74.03 and 80.30 mg, respectively).  相似文献   

8.
Coupled photosynthesis-stomatal conductance (A-gs) models are commonly used in ecosystem models to represent the exchange rate of CO2 and H2O between vegetation and the atmosphere. The ways these models account for water stress differ greatly among modelling schemes. This study provides insight into the impact of contrasting model configurations of water stress on the simulated leaf-level values of net photosynthesis (A), stomatal conductance (gs), the functional relationship among them and their ratio, the intrinsic water use efficiency (A/gs), as soil dries. A simple, yet versatile, normalized soil moisture dependent function was used to account for the effects of water stress on gs, on mesophyll conductance (gm) and on the biochemical capacity. Model output was compared to leaf-level values obtained from the literature. The sensitivity analyses emphasized the necessity to combine both stomatal and non-stomatal limitations of A in coupled A-gs models to accurately capture the observed functional relationships A vs. gs and A/gsvs. gs in response to drought. Accounting for water stress in coupled A-gs models by imposing either stomatal or biochemical limitations of A, as commonly practiced in most ecosystem models, failed to reproduce the observed functional relationship between key leaf gas exchange attributes. A quantitative limitation analysis revealed that the general pattern of C3 photosynthetic response to water stress may be well represented in coupled A-gs models by imposing the highest limitation strength to gm, then to gs and finally to the biochemical capacity.  相似文献   

9.
This study was undertaken to investigate how different cultivars of sweet basil (Ocimum basilicum) responded to boron (B) excess. Two purple‐leafed and eight green‐leafed cultivars were hydroponically grown for 20 d with 0.2 or 20 mg L–1 B in the nutrient solution. Leaf B concentration, gas exchanges, chlorophyll a fluorescence, and oxidative stress were determined at the end of the treatment along with the severity of leaf necrosis. A range of tolerance to B toxicity was found: the green cultivars were more susceptible than the purple‐leafed ones characterized by a higher constitutive anthocyanin concentration. In all the genotypes B excess resulted in oxidative stress as determined by accumulation of malondialdehyde by‐products (MDA), reduced photosynthesis, and the occurrence of leaf burn. A close correlation was found between leaf B accumulation and oxidative stress, as well as between oxidative stress and the severity of leaf burn. Net photosynthesis (Pn) was reduced due to both stomatal and nonstomatal limitations in the green cultivars whereas the reduction of Pn in the purple leaves was only attributable to stomatal factors. Chlorophyll a fluorescence revealed a decrease in the maximum quantum yield of PSII (Fv/Fm) and in the electron transport rate (ETR) in plants grown with B excess although less reduction was observed in the purple genotypes. The quantum yield of PSII (ΦPSII) decreased as a result of B toxicity only in the green cultivars. It is concluded that anthocyanins are involved in attenuation of the negative effects of B toxicity.  相似文献   

10.
The deficiency of potassium (K) has resulted in decreasing the yield and quality of food grains. Moreover, with decreasing water resources the use of wastewater in agriculture as an alternative source of water and nutrients is being debated. This study was therefore undertaken to test wastewater for its suitability as irrigation water together with uniform basal doses of nitrogen (N) and phosphorus (P) and varying doses of potassium (K0, K20, K40 and K60). It was observed that the plants receiving wastewater as a source of irrigation water performed better with a lower K dose. Potassium at 20?kg?ha?1 along with wastewater resulted in better growth, photosynthetic rate (P N), stomatal conductance (g s) and yield of the chickpea (Cicer arietinum L.). Thus fertilizer rates could be lowered with the use of wastewater which can serve not only as the source of water but of nutrients also. However, regular monitoring of wastewater and soil for any buildup of heavy metal is necessary. The physical and chemical parameters of wastewater were also tested and most of them were found to be well within the permissible limits as set by the Food and Agriculture Organization (FAO).  相似文献   

11.
Abstract

Influence of boron (B) on photosynthesis and water use has not sufficiently been evaluated in soybean despite its worldwide importance as a crop. The objective of this work was to evaluate the effects of B application on photosynthesis and water use in soybean at vegetative growth stage. A pot experiment was carried out in a greenhouse. Soybean [Glycine max (L.) Merrill cv. M8644 IPRO] was grown in a clayey Oxisol previously fertilized with 4 B rates (0, 1.5, 3.0, and 6.0?mg dm?3). Net photosynthesis rate (Pn), intercellular CO2 concentration (Ci), transpiration rate (E), stomatal conductance (gs) were measured in the second trifoliate leaf of plants at the V4 growth stage. Instantaneous water use efficiency (WUEis = Pn/E) and intrinsic water use efficiency (WUEic = Pn/gs) were calculated. B application to soil increased all these variables. The most consistent increases were in Pn, WUEis, and WUEic. The critical level of hot water extractable B in soil that maximized photosynthesis and optimized water use by soybean at vegetative growth stage was calculated to be 0.38?mg dm?3.  相似文献   

12.
Abstract

The olive tree (Olea europaea L.) is adapted to tolerate severe drought and high irradiance levels. Relative electron transport rate (J), photosynthetic efficiency (in terms of F v /F m and ΦPSII), photochemical (qP) and non-photochemical quenching (NPQ) were determined in 2-year old olive plants (cultivars ‘Coratina’ and ‘Biancolilla’) grown under two different light levels (exposed plants, EP, and shaded plants, SP) during a 21-day controlled water deficit. After reaching pre-dawn leaf water potentials of about -6.5 MPa, plants were rewatered for 23 days. During the experimental period, measurements of gas exchange and chlorophyll fluorescence were carried out to study the photosynthetic performance of olive plants. The effect of drought stress and high irradiance levels caused a reduction of gas exchange, J, ΦPSII and F v/F m and this decrease was more marked in EP. Under drought stress, EP showed a higher degree of photoinhibition, a higher NPQ and a lower qP if compared to SP. Coratina was more sensitive to high light and drought stress and had a slower recovery during rewatering. The results confirm that photoprotection is an important factor that affects photosynthetic productivity in olive, and that the degree of this process varies between the cultivars. This information could give a more complete picture of the response of olive trees grown under stressful conditions of semi-arid environments, and could be important for the selection of drought-tolerant cultivars with a high productivity.  相似文献   

13.
ABSTRACT

To characterize the effect of silicon (Si) on decreasing transpiration rate in maize (Zea mays L.) plants, the transpiration rate and conductance from both leaves and cuticula of maize plants were measured directly. Plants were grown in nutrient solutions with and without Si under both normal water conditions and drought stress [20% polyethylene glycol (PEG) concentration in nutrient solution] treatments. Silicon application of 2 mmol L?1 significantly decreased transpiration rate and conductance for both adaxial and abaxial leaf surface, but had no effect on transpiration rate and conductance from the cuticle. These results indicate that the role of Si in decreasing transpiration rate must be largely attributed to the reduction in transpiration rate from stomata rather than cuticula. Stomatal structure, element deposition, and stomatal density on both adaxial and abaxial leaf surfaces were observed with scanning electron microscopy (SEM) and a light microscope. Results showed that changes in neither stomatal morphology nor stomatal density could explain the role of Si in decreasing stomatal transpiration of maize plants. Silicon application with H4SiO4 significantly increased Si concentration in shoots and roots of maize plants. Silicon concentration in shoots of maize plants was higher than in roots, whether or not Si was applied. Silicon deposits in cell walls of the leaf epidermis were mostly in the form of polymerized SiO2.  相似文献   

14.
Sorghum [Sorghum bicolor (L.) Moench] was grown hydroponically with or without 50 ppm silicon (Si), and exposed to water stress from 10 days after sowing (DAS). At 15 and 23 DAS, we measured dry weight and diurnal variations in photosynthetic rate (P N), stomatal conductance (g s), transpiration rate (T), leaf water potential (ψ L), and water uptake rate (23 DAS only). The reduction in dry weight at 23 DAS caused by water stress was ameliorated by silicon. Under water stress, silicon-treated seedlings showed higher g s, P N, and T than untreated ones. ψ L remained almost constant within treatments throughout the daytime. Water uptake rate was reduced by water stress, but the reduction was ameliorated by silicon. We conclude that silicon enhanced water uptake and g s, improving water supply to the leaves. These effects of silicon occurred soon after exposure to water stress.  相似文献   

15.
Enhancing crop water‐use efficiency (WUE) is a major research objective in water‐scarce agroecosystems. Potassium (K) enhances WUE and plays a crucial role in mitigating plant stress. Here, effects of K supply and PEG‐induced water deficit on WUE of spring wheat (Triticum aestivum L. var. Sonett), grown in nutrient solution, were studied. Plants were treated with three levels of K supply (0.1, 1, 4 mM K+) and two levels of PEG (0, 25%). WUE was determined at leaf level (WUEL), at whole‐plant level (WUEP), and via carbon isotope ratio (δ13C). Effects of assimilation and stomatal conductance on WUEL were evaluated and compared with effects of biomass production and whole‐plant transpiration (EP) on WUEP. Adequate K supply enhanced WUEP up to 30% and by additional 20% under PEG stress, but had no effect on WUEL. EP was lower with adequate K supply, but this effect may be attributed to canopy microclimate. Shoot δ13C responded linearly to time‐integrated WUEL in adequately supplied plants, but not in K‐deficient plants, indicating negative effects of K deficiency on mesophyll CO2 diffusion. It is concluded that leaf‐scale evaluations of WUE are not reliable in predicting whole‐plant WUE of crops such as spring wheat suffering K deficiency.  相似文献   

16.
The objective of this study was to determine drought tolerance characteristics of dryland wheat genotypes based on leaf gas exchange and water-use efficiency in order to identify promising genotypes for drought tolerance breeding. Physiological responses of ten genetically diverse wheat genotypes were studied under non-stressed (NS) and water stressed (WS) conditions using a 2?×?10 factorial experiment replicated 3 times. A highly significant water condition?×?genotype interaction (P?<?0.001) was observed for photosynthetic rate (A), ratio of photosynthetic rate and internal CO2 concentration (A/Ci), ratio of internal and atmospheric CO2 (Ci/Ca), intrinsic (WUEi) and instantaneous (WUEinst) water-use efficiencies suggesting genotypic variability among wheat genotypes under both test conditions. Principal component analysis (PCA) identified three principal components (PC’s) under both test conditions accounting for 84% and 89% of total variation, respectively. Bi-plot analysis identified G339 and G344 as drought tolerant genotypes with higher values of A, T, gs, A/Ci, WUEi and WUEinst under WS condition. The current study detected significant genetic variation for drought tolerance among the tested wheat genotypes using physiological parameters. Genotypes G339 and G344 were identified to be drought tolerant with efficient A, T, gs, A/Ci and water-use under water stressed condition.  相似文献   

17.
Sunflower plants (Helianthus annuus L. cv. Zwerg Sonnengold) were cultivated in pots containing 1 kg of soil fertilized with 0.6 g K (K1) and 5.0 g K/pot (K2). At the age of 5 and 11 weeks, respectively the plants were transferred to a growth chamber. 50% of the plants per K treatment received sufficient watering (controls), the remaining 50% were subjected to water stress until visible signs of severe wilting were observed. At that stage the plants were rewatered. In both growth stages and even under water stress conditions the up to 5 times higher K concentrations in the tissue of K2 plants caused an increased dry matter production during the experimental periods and a larger total leaf area which was less reduced under water stress than the leaf area of K1 plants. During continuous watering the water content per leaf area of K2 plants was on the average 1.5g H2O dm?2 (K1: 1.35g), the water content of older K2 plants being on the average 1.33g H2O dm?2 (K1: 1.25g). During water stress and subsequent recovery this relation observed between K2 and K1 remained constant. In young K1 plants, however, no intensified loss of succulence was found during severe drought compared with 11-week-old K1 plants where the water content decreased from 1.2g to 0.6g H2O per dm2 of leaf area. At almost equally high soil water availability stomatal diffusive resistance especially of the older unstressed K2 plants was higher than in K-deficient plants. Under water stress, the degree of stomatal opening of K2 plants at first decreased more rapidly, but at equally low soil water potentials diffusive resistance in the leaves of K2 plants remained lower than in K1 plants. A relation of the leaf water characteristics, total water potential (41), osmotic potential (42), and turgor potential (Φp) to the corresponding soil water potential showed that under stress Φ1 and Φs in plants with sufficient K nutrition always remained on a higher level than in K1 plants. Consequently, they were able to maintain a higher turgor pressure even under conditions of restricted water availability.  相似文献   

18.
In central India, four populations of groundnut were grown to assess the interaction between population and water stress.Transpiration was calculated from measurements or estimates of stomatal resistance, rs, boundary layer resistance, ra, vapour concentration difference between leaf and air, δχ, and leaf area index, L.The frequency distributions of rs, ra, δχ and seasonal changes in L were plotted to analyse the dependence of transpiration rate on each variable, both per unit area of leaf surface, El, and per unit land surface, Ee. For estimates of El, both rs and δχ were of similar importance, exerting a far greater influence than changes in ra. However, in terms of Ee, changes in L were far more important than in any other variable, particularly late in the season when water was scarce.This study provides further experimental support for estimates of evaporation based on stomatal resistance and allied measurements and confirms similar estimates obtained earlier for crops of pearl millet maintained on stored water. The ability of the technique to describe temporal and spatial variations as well as the dominant environmental and physiological influences on transpiration may outweigh any small loss in accuracy of estimates thus obtained.  相似文献   

19.
ABSTRACT

The interactions between salinity and different nitrogen (N) sources nitrate (NO3 ?), ammonium (NH4 +), and NO3 ? + NH4 + were investigated on Indian mustard (Brassica juncea cv. RH30). Treatments were added to observe the combined effect of two salinity levels (8 and 12 ds m? 1) and three nitrogen sources (NO3 ?, NH4 +, and NO3 ? + NH4 +) on different growth parameters and mineral composition in different plant parts, i.e., leaves, stem, and root. Salinity has been known to affect the uptake and assimilation of various essential nutrients required for normal growth and development. Different growth parameters, i.e., leaf area, dry weight of different plant parts, absolute growth rate (AGR), relative growth rate (RGR), and net assimilation rate (NAR) declined markedly by salinity at pre-flowering and flowering stages. All growth indices were less sensitive to salinity (12 d s m? 1) with the nitrate form of nitrogen. It is pertinent mention that a high dose (120 kg ha? 1) of nitrogen in ammonium form NH4 +, acted synergistically with salinity in inhibiting growth. Plants fed with combined nitrogen (NO3 ? + NH4 +) had an edge over individual forms in ameliorating the adverse effects of salinity on growth and yield. Under salt stress, different nutrient elements such as N, phosphorus (P), potassium (K+), and magnesium (Mg2 +) were decreased in different plant parts (leaves, stem, and root). The maximum and minimum reduction was observed with ammoniacal and combined form of nitrogen, respectively, while the reverse was true of calcium (Ca2 +), sodium (Na+), chloride (Cl?), and sulfate (SO4 2?) at harvest. Nitrogen application (120 Kg ha? 1) in combined form had been found to maintain highest concentrations of N, P, Mg2 +, and Ca2 + along with reduced concentrations of Na+, Cl?, and SO4 2 ?. However, reverse was true with ammoniacal form of nitrogen.  相似文献   

20.
Understanding the effect of boron (B) on plant physiology will help to refine the diagnosis of B deficiency and improvement in B fertilizer recommendations for cotton (Gossypium hirsutum L.) growing areas. This study shows the testing of hypotheses “that application of B-fertilizer improves net photosynthetic rate (PN) and water use efficiency (WUE) for cotton plant on a B-deficient soil [< 0.50 mg B kg?1 hydrochloric acid (HCl)-extractable] in an arid environment”. Thus, a permanent layout [two-year field experiment (2004 and 2005)] was conducted to study the impact of B fertilizer at 0, 1.0, 1.5, 2.0, 2.5, and 3.0 kg ha?1 on gas exchange and electrolyte leakage (EL) characteristics of cotton crop (cv. ‘CIM-473’). The soil at experimental site was alkaline (pH 8.1), calcareous [calcium carbonate (CaCO3 5.6%)], and silt loam (Typic Haplocambid). Boron use decreased EL of plant membrane (P ≤ 0.05), and increased PN, transpiration rate (E) and stomatal conductance (gs), while intercellular concentration of carbon dioxide (CO2; Ci) significantly decreased (P ≤ 0.05) during both experimental years. There was a positive, but non-significant effect of B concentration on chlorophyll content in plant leaves. Application of 3.0 kg B ha?1 improved WUE up to 9.7% [4.62 μmol (CO2) mmol?1 water (H2O)] compared to control plants (4.21 [μmol (CO2) mmol?1 (H2O)]. Principal component analysis (PCA) of data indicates positive correlations between leaf B concentration and PN, E, gs, and WUE, while a negative relationship existed between leaf B concentration and intercellular CO2 (Ci). This study showed that addition of B fertilizer in the B-deficient calcareous soil proved beneficial for growth and development for cotton crop by enhancing its WUE and gas exchange characteristics.  相似文献   

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