共查询到20条相似文献，搜索用时 23 毫秒
1.
Sang-Nag Ahn Yeon-Kyu Kim Ha-Cheol Hong Seong-Sook Han Soo-Jin Kwon Hae-Chune Choi Huhn-Pal Moon Susan R. McCouch 《Euphytica》2000,116(1):17-22
A single dominant blast resistance gene conferring resistance to a Korean rice blast isolate was identified in rice variety
`Suweon 365'. We report the chromosomal localization and molecular mapping of this blast resistance gene designated as Pi-18, which confers resistance to Korean isolate `KI-313' of the blast pathogen. To know whether there is a relationship among
genes conditioning resistance to location-specific isolates of the blast pathogen and thereby to identify linked markers to
resistance gene for isolate KI-313 collected in Korea, RFLP markers previously reported to be linked to major blast resistance
genes in different rice germplasm and other markers mapped to nearby regions were surveyed for polymorphism between a resistant
(`Suweon 365') and a susceptible (`Chucheongbyeo') parent. Linkage associations of the RFLP markers with the resistance gene
were verified using an F2 and F3 segregating population of known blast reaction. RFLP analysis showed that Pi-18 was located near the end of chromosome 11, linked to a single copy clone RZ536 at a distance of 5.4 centiMorgans (cM) and
that this gene was different from Pi-1(t). An allelism test revealed that this gene was also different from Pi-k. Currently, a combination of RAPD and microsatellite primers is being employed to find additional markers in this region.
Tightly linked DNA markers will facilitate selection for resistant genotypes in breeding programs and provide the basis for
map based cloning of this new blast resistance gene.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
2.
水稻黑条矮缩病是水稻主要病毒病害之一。目前由于缺乏规模、高效的黑条矮缩病抗性鉴定体系,制约了抗黑条矮缩病水稻资源的发掘,限制了抗黑条矮缩病的育种进程和基础研究。本研究通过分析水稻黑条矮缩病田间鉴定所需灰飞虱的有效接种密度、带毒率及播期等,提出水稻黑条矮缩病田间鉴定有效接种的灰飞虱密度在800万头 hm-2左右较为合理,而带毒率应不低于5%。并进一步对现有黑条矮缩病人工接种鉴定的循回期、接种虫量、接种时间及虫龄等进行了优化。利用上述鉴定体系,2010年对来源于20个国家的共1240份水稻种质进行黑条矮缩病田间鉴定,初步获得发病率低于10%的品种34个;2011、2012连续两年对该34个品种进行多年多点重复抗性鉴定,发现来自东南亚地区的3个品种Kanyakumari 29、Madurai 25和Vietnam 160连续3年发病率均低于10%,表现较高的黑条矮缩病的抗性。进一步分期播种鉴定的结果表明,Kanyakumari29在3个播期、3个鉴定点的发病率均低于12%,而Madurai 25和Vietnam 160发病率均低于9%。此外,在人工接种条件下Kanyakumari 29、Madurai 25和Vietnam 160的发病率均低于9%。因此,多年多点田间鉴定和人工室内接种鉴定的结果均表明,Kanyakumari 29、Madurai 25和Vietnam 160稳定、高抗黑条矮缩病。综上所述,本研究建立的田间鉴定与室内鉴定相结合的黑条矮缩病鉴定体系准确、可靠,可用于黑条矮缩病的大规模鉴定,该体系的建立及高抗黑条矮缩病水稻资源的发掘为水稻抗黑条矮缩病基因的鉴定及育种利用提供了重要的方法和材料基础。 相似文献
3.
We investigated the mode of inheritance and map location of field resistance to rice blast in the elite rice strain Chubu 111, and yield under severe blast conditions. Chubu 111 carries the complete resistance gene Pii, although field testing showed this strain to be susceptible to infection. The level of field resistance of Chubu 111 was so high that chemicals used to control blast were not required, even in an epiphytotic area. Genetic analysis of field resistance to blast in 149 F3 lines derived from a cross between Chubu 111 and the susceptible cultivar ‘Mineasahi’ suggested that field resistance is controlled by a dominant gene, designated Pi39(t), that cosegregates with the single sequence repeat marker loci RM3843 and RM5473 on chromosome 4. Comparative studies of polymorphism at RM3843 among Chubu 111 and six cultivars or lines in its pedigree suggested that the donor of the resistance gene was the Chinese cultivar ‘Haonaihuan’. Marker‐assisted selection of Pi39(t) should be useful in rice‐breeding programmes for field resistance to blast. 相似文献
4.
Homozygous transgenic rice lines expressing GNA with enhanced resistance to the rice sap-sucking pest Laodelphax striatellus 总被引：5，自引：0，他引：5
Homozygous transgenic rice lines expressing snowdrop lectin, Galanthus nivalis agglutinin (GNA), were investigated for resistance to the rice small brown planthopper (SBPH), Laodelphax striatellus, an important rice sap‐sucking pest causing yield losses and serving as vector for some important rice viruses. Insect bioassay results revealed that both the GNA‐expressing homozygous lines tested significantly inhibited SBPH by decreasing its survival, its overall fecundity and retarding development. This is the first report on homozygous transgenic rice lines expressing GNA conferring enhanced resistance to SBPH. This result suggests that transgenic rice expressing GNA will be useful in rice pest resistance breeding, as an alternative to conventional breeding for the control of SBPH, and potentially alleviating damage caused by the viruses it transmits. 相似文献
5.
利用Mudgo/武育粳3号F2群体分析水稻抗灰飞虱QTL 总被引：1，自引：0，他引：1
灰飞虱是我国水稻生产上的重要害虫。Mudgo是一个高抗灰飞虱的籼稻品种,对灰飞虱具有强的排驱性和抗生性抗性。利用Mudgo/武育粳3号F2群体,构建了含有177个单株的F2群体的遗传连锁图谱。该连锁图包含104个SSR标记和3个Indel标记,覆盖整个水稻基因组1 409.9 cM,每两个标记之间的平均距离为13.2 cM。采用改进的苗期集团筛选法对177个F2:3家系进行了抗性鉴定,通过Windows QTL Cartographer 2.5进行复合区间作图分析,在第2、3、12染色体上分别检测到抗灰飞虱QTL Qsbph2b、Qsbph3d和Qsbph12a,分别位于标记RM5791~RM29、RM3199~RM5442和I12-17~RM333 1之间,单个LOD值分别为3.25、3.11和6.82,贡献率分别为17.3%、15.6%和35.8%,各QTL增强抗性的等位基因效应均来自Mudgo。其中Qsbph12a与标记RM3331和I12-17紧密连锁。结合表型鉴定的结果,Qsbph12a应为抗灰飞虱主效QTL,与该位点紧密连锁的标记可用于抗灰飞虱快速选择辅助育种。 相似文献
6.
Summary Lesion size and lesion number were measured on cultivars of rice inoculated by clipping or spraying with virulent isolates of Xanthomonas campestris pv. oryzae. Mean percentage diseased leaf area (%DLA) gave a similar ranking for the two inoculation methods but differences in lesion size among cultivars were much more evident after clip than after spray inoculation. Correlation between the methods was high (r=0.82**), but some cultivars responded differently with the two techniques. Cultivars which had low scores following spray inoculation showed low disease progress during the first nine weeks after transplanting into a screen-house experiment. Assessment after clip inoculation measures resistance due to spread of bacteria within the leaf xylem system, an important component of quantitative resistance. Assessment after spray inoculation measures all resistance, including resistance to entrance of bacteria into the leaf. In order to select rice entries with improved quantitative resistance to X. c. oryzae based on both components, a screening based on lesion length after clip inoculation, followed by a test for lesion number after spray inoculation, is advised. 相似文献
7.
J. M. Bonman 《Euphytica》1992,63(1-2):115-123
Summary Blast is one of the most serious diseases of rice worldwide. The pathogen,Pyricularia grisea, can infect nearly all parts of the shoot and is commonly found on the leaf blade and the panicle neck node. Host resistance is the most desirable means of managing blast, especially in developing countries. Rice cultivars with durable blast resistance have been recognized in several production systems. The durable resistance of these cultivars is associated with polygenic partial resistance that shows no evidence of race specificity. This partial resistance is expressed as fewer and smaller lesions on the leaf blade but latent period does not appear to be an important component. Partial resistance to leaf blast is positively correlated with partial resistance to panicle blast, although some cultivars have been found showing leaf-blast susceptibility and panicle-blast resistance. A diverse set of environmental factors can influence the expression of partial resistance, including temperature, duration of leaf-wetness, nitrogen fertilization, soil type, and water deficit. Because of the great diversity of rice-growing environments, resistance that proves durable in one system may or may not prove useful in another. In highly blast-conducive environments, other means of disease management must be applied to assist host-plant resistance. 相似文献
8.
Jorge Luis Fuentes Fernando José Correa-Victoria Fabio Escobar Gustavo Prado Girlena Aricapa Myriam Cristina Duque Joe Tohme 《Euphytica》2008,160(3):295-304
The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from
the Gramene database (www. gramene.org) considering their relative proximity to Pi-1(t) gene in the current rice genetic map. Progenitors and DNA bulks of resistant and susceptible families from F3 segregating populations of a cross between the near-isogenic lines C101LAC (resistant) and C101A51 (susceptible) were used
to identify polymorphic microsatellite markers associated to this gene through bulked segregant analysis. Putative molecular
markers linked to the blast resistance gene Pi-1(t) were then used on the whole progeny for linkage analysis. Additionally, the diagnostic potential of the microsatellite markers
associated to the resistance gene was also evaluated on 17 rice varieties planted in Latin America by amplification of the
specific resistant alleles for the gene in each genotype. Comparing with greenhouse phenotypic evaluations for blast resistance,
the usefulness of the highly linked microsatellite markers to identify resistant rice genotypes was evaluated. As expected,
the phenotypic segregation in the F3 generation agreed to the expected segregation ratio for a single gene model. Of the 24 microsatellite sequences tested, six
resulted polymorphic and linked to the gene. Two markers (RM1233*I and RM224) mapped in the same position (0.0 cM) with the
Pi-1(t) gene. Other three markers corresponding to the same genetic locus were located at 18.5 cM above the resistance gene, while
another marker was positioned at 23.8 cM below the gene. Microsatellite analysis on elite rice varieties with different genetic
background showed that all known sources of blast resistance included in this study carry the specific Pi-1(t) allele. Results are discussed considering the potential utility of the microsatellite markers found, for MAS in rice breeding
programs aiming at developing rice varieties with durable blast resistance based on a combination of resistance genes.
Centro Internactional de Agricultura Tropical (CIAT) institute where the research was carried out 相似文献
9.
10.
稻瘟病苗瘟叶瘟和穗颈瘟的相关性分析 总被引：8，自引：0，他引：8
采用温室人工接菌法探讨水稻稻瘟病苗瘟、叶瘟和穗颈瘟的相关性,研究结果表明,稻瘟病苗瘟、叶瘟和穗颈瘟三者之间存在着一定的正相关性,相关程度因水稻品种、稻瘟病菌生理小种的不同而有所差异。此外,对水稻品种温室苗瘟抗性率与田间叶瘟抗性率、穗颈瘟抗性率之间的相关性进行研究,结果表明温室苗瘟抗性率与自然病圃叶瘟抗性率表现正相关关系(r=0.5435),温室苗瘟抗性率与自然病圃穗颈瘟抗性率表现极显著正相关关系(r=0.7583**,p<0.01),自然病圃叶瘟抗性率与穗颈瘟抗性率呈显著正相关关系(r=0.6322*,p<0.05)。因此,认为利用可控温室代替田间进行水稻品种稻瘟病抗性鉴定是可行的,而且根据苗瘟、叶瘟的抗性鉴定结果推测穗颈瘟的抗性基本上也是可行的。 相似文献
11.
Jahangir Imam Shamshad Alam Nimai P. Mandal Mukund Variar Pratyoosh Shukla 《Euphytica》2014,196(2):199-211
Molecular screening and genetic diversity of major rice blast resistance (R) genes were determined in 32 accessions of rice germplasm from North East and Eastern India with ten gene based single nucleotide polymorphisms and sequence tagged sites (STS) markers, namely z56592, zt56591, k39512, k3957, candidate gene marker, Pita3, YL155/YL87, YL183/YL87, Pb28, 195R-1 which showed close-set linkage to nine major rice blast resistance (R) genes, Piz, Piz-t, Pik, Pik-p, Pik-h, Pita/Pita-2, Pib and Pi9 and one susceptible pita gene. Among the 32 accessions, 13 were positive for Piz gene and six for Piz-t gene. Six accessions were positive for Pik gene, seven for Pik-p and 16 for Pik-h gene. One accession, Atte thima, was positive for three of Pik multiple genes. Out of 32, only two germplasm, Dudhraj and Nepali dhan, were detected with both Pita3 and YL155/YL87 marker for Pita/Pita-2 gene. The Pib gene appeared to be omnipresent and was detected in 31 of 32 germplasm with marker Pb28. The gene specific STS marker, 195R-1, for Pi9 gene produced positive bands in only two germplasm, Kalchatti and Bachi thima. The Uniform Blast Nursery (UBN) analysis showed that out of 32, six germplasm was resistant, ten moderately resistant and 16 germplasm were susceptible. Presence of Piz-t, Pita/Pita-2 and Pi9 gene ensured a resistant reaction in outdoor blast nursery whereas germplasm carrying Pib was susceptible when present alone. Presence of multiple genes, however, contributed to slow blasting resistance in the field. These results are useful in identification and incorporation of resistant genes from the germplasm into elite cultivars through marker assisted selection in rice breeding programs. 相似文献
12.
Atitaya Chumpol Sompong Chankaew Suwita Saepaisan Tidarat Monkham Jirawat Sanitchon 《Euphytica》2018,214(10):183
Upland rice production plays an important role in both household consumption and crop rotation. Until now, a blast resistant upland variety has not been released in Thailand. The bimodal pattern of rain distribution in Thailand’s upland rice production areas create a favorable environment for the outbreak of leaf blast when seedling-tillering, and neck blast within the heading stage. The use of genetically resistant cultivars has proven to be an effective way to cope with this problem. In this study, 256 indigenous upland rice plants were screened for blast resistance under greenhouse and field conditions. Ten indigenous upland rice varieties, ULR292, ULR242, ULR219, ULR162, ULR161, ULR134, ULR109, ULR098, ULR081, and ULR066, were identified as resistant to leaf blast disease in both natural infection and artificial inoculation, under greenhouse conditions. Additionally, six of the ten varieties, ULR162, ULR161, ULR134, ULR109, ULR098, and ULR081, were found to be resistant to neck blast under field conditions. These new sources of blast resistance identified from indigenous upland rice varieties proved more resistant than the check varieties, depicting their potential for further use in Thailand’s rice blast resistance improvement program. 相似文献
13.
广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应 总被引：4，自引：0，他引：4
Pigm是Pi2基因簇的等位或紧密连锁基因。本研究构建了4个不同遗传背景下Pigm和Pi2的系列近等基因系,204个菌株苗期接种结果显示其抗性频率均超过70%,但Pigm和Pi2的抗谱重叠度仅为54.4%~65.7%,聚合Pi1/Pigm和Pi1/Pi2杂种的抗性频率均超过90%。穗瘟人工接种及病圃自然诱发鉴定表现与苗期接种一致的发病趋势。农艺性状调查结果显示获得的近等基因系与其轮回亲本基本相似,存在较少的累赘连锁。表明Pigm是一个与Pi2抗谱差异明显的广谱抗性基因,对稻瘟病抗性育种具有重要应用价值。 相似文献
14.
M. N. Shrivastava Arvind Kumar Sandeep Bhandarkar B. C. Shukla K. C. Agrawal 《Euphytica》2003,130(1):143-145
The Asian rice gall midge, Orseolia oryzae Wood Mason (Diptera: Cecidomyiidae), is a major pest of rice in several South and South East Asian countries. The maggots
feed internally on the growing tips of the tillers and transform them into tubular galls, onion leaf-like structures called
‘silver shoots’ resulting into severe yield loss to the rice crop. We studied the mode of inheritance and allelic relationships
of the resistance genes involved in resistant donor Line 9, a sib of a susceptible cultivar ‘Madhuri’. The segregation behaviour
of F1, F2 and F3 populations of the cross between Line 9 and susceptible cultivar MW10 confirmed the presence of a single dominant gene for
resistance. Tests of allelism with all the known genes giving resistance to this population indicated that Line 9 possessed
a new gene which was designated Gm 9
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
15.
Downy mildew, incited by Peronospora parasitica (Pers.: Fr.) Fr., is a destructive disease of broccoli (Brassica oleraceaL., Italica Group). Resistant cultivars represent a desirable control method to provide a practical, environmentally benign,
and long-term means of limiting damage from this disease. Doubled-haploid (DH) lines developed by us exhibit a high level
of downy mildew resistance at the cotyledon stage. To determine the mode of inheritance for this resistance, a resistant DH
line was crossed to a susceptible DH line to make an F1, from which F2 and backcross (BC) populations were developed. All populations were evaluated for response to artificial inoculation with
P. parasitica at the cotyledon stage. All F1 plants (including reciprocals) were as resistant as the resistant parent, indicating no maternal effect for this trait. F2 populations segregated approximately 3resistant to 1 susceptible, BC populations using the resistant parent as the recurrent
parent contained all resistant plants, and the BC to the susceptible parent segregated 1 resistant to 1 susceptible. These
results indicate that resistance is controlled by a single dominant gene. This gene should be easily incorporated into F1 hybrids and used commercially to prevent downy mildew at the cotyledon stage.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
16.
Summary Rice cultivars (Oryza sativa L.) belonging to five ecotypes (aus, aman, boro, bulu and tjereh) and to two groups of Japanese rice (lowland and upland) are examined with respect to KClO3 resistance, phenol reaction and apiculus hair length. These characters have been used as available criteria to classify rice into two types indica and japonica, for the last thirty years.The findings of this study are that the aman, boro and tjereh ecotypes should be classified as typical indica; and that the Japanese lowland rice cultivars are mainly typical japonica. Some of the aus, bulu and Japanese upland rice cultivars differ from typical indica and typical japonica, so the respective terms aus type, bulu type and J.u.r. type, are proposed.
Aman, boro tjereh and Japanese lowland rice are cultivated in lowland. Some of the aus, bulu and Japanese upland rice cultivars have the characteristics of upland rice. In general, lowland rice cultivars can be clearly classified into indica or japonica, while upland cultivars cannot.Abbreviations
Aph
dominant gene for apiculus hair length > 0.7 mm
-
aph
recessive gene for apiculus hair length < 0.7 mm
-
J.u.r. type
Japanese upland rice type 相似文献
17.
Molecular mapping of a novel blast resistance gene Pi38 in rice using SSLP and AFLP markers 总被引：2，自引：0，他引：2
Blast, caused by Magnaporthe grisea, is the most devastating disease of rice worldwide. In this study, the main objective was to identify and map a new gene for blast resistance, in an indica rice cultivar ‘Tadukan’ against blast fungal isolate B157, using molecular tools. F2 segregating population was derived from ‘CO39’ (susceptible) and ‘Tadukan’ (resistant), and molecular mapping of the blast resistance gene was carried out using simple sequence length polymorphism (SSLP) and amplified fragment length polymorphism (AFLP) methods. Two SSLP markers, RM206 and RM21 and three AFLP markers (AF1: E‐aca/M‐ctt; AF2: E‐aca/M‐cat and AF3: E‐acc/M‐cac2) were identified to be linked to the resistance gene. The co‐segregation analysis using SSLP markers implied that the blast resistance gene designated Pi38 resides on rice chromosome 11. 相似文献
18.
Rice blast resistance gene ‘Pi-z’ present in rice genotypes, Zenith and Fukunishiki, represents a potential source of blast resistance for the north-western
Himalayan region of India. We tested the reliability of microsatellite markers linked to Pi-z for assessing blast resistance phenotype in crosses of commercial importance. A new set of microsatellite markers linked
to Pi-z was also developed by exploiting the publicly available marker and genomic resources of rice. Of the three previously reported
markers for Pi-z, only MRG5836 was suitable for the marker assisted selection of Pi-z. Among the 17 microsatellites selected from the putative region of Pi-z locus, two, RM8225 and RM8226 cosegregated with MRG5836 and were located at distance of 1.2–4.5 cM from the gene. A new microsatellite marker ‘SSR236’ was developed from the (CT)16 repeat of PAC clone P0502B12, which exhibited closer linkage (0.6–1.2 cM) to Pi-z. Survey of the allelic diversity at the loci of the Pi-z linked microsatellite markers revealed that the Fukunishiki and Zenith type alleles were not present in majority of the local
indica rice genotypes. As these markers are polymorphic between the Pi-z donors and a great majority of local indica rices tested, they can be used as a selection tool in rice breeding programs aimed at improving the blast resistance of local
rices. 相似文献
19.
The Pi‐ta gene in rice confers resistance to strains of the blast pathogen Magnaporthe grisea (Herbert) Borr. (anamorph Pyricularia oryza Cav.) containing the corresponding avirulence gene AVR‐Pita in a gene‐for‐gene fashion. The Pi‐ta gene is a typical nucleotide‐binding site type resistance gene. Nucleotide sequences distinguishing the resistant Pi‐ta and susceptible pi‐ta alleles were previously identified and used for developing DNA markers for a resistant Pi‐ta haplotype and three susceptible pi‐ta haplotypes. In the present study, the existence of the Pi‐ta gene in 141 rice germplasm accessions was rapidly determined using these markers, and the results were confirmed by inoculating rice germplasm with an M. grisea strain containing AVR‐Pita. The Pi‐ta gene was found in accessions from several major rice producing countries, including China, Colombia, Japan, Vietnam, the Philippines, Iran and the United States. The usefulness of DNA markers for rapid determination of the genotype of rice germplasm was thus demonstrated. The Pi‐ta gene also was found in rice cultivar known to contain the Pi‐ta2 gene, although the allelic relationship of these genes remains to be determined. The presence of the Pi‐ta gene in landrace cultivars in several different geographical locations, the Philippines and Vietnam, other indica rice cultivars in China and Colombia suggest that the Pi‐ta gene may have spontaneously originated in indica rice cultivars. These results are useful for incorporating the Pi‐ta gene into advanced breeding lines by marker‐assisted selection for rice breeding programmes worldwide. 相似文献
20.
水稻抗条纹叶枯病基因Stv-bi的分子标记辅助选择 总被引：1，自引：0，他引：1
水稻条纹叶枯病是我国黄淮及长江流域粳稻区重要的病害。由于水稻条纹叶枯病的发病受外界条件影响较大,人工接种抗性鉴定比较困难,利用与抗病基因紧密连锁的分子标记进行标记辅助选择对提高抗性育种效率具有重要意义。来自籼稻抗源Modan的Stv-bi是水稻育种中广泛应用的条纹叶枯病抗性基因。本研究设计了与Stv-bi紧密连锁的SSR及STS分子标记,用3个抗条纹叶枯病混合群体F30718(圣稻13/镇稻88)、F50701(武优34/T022//圣稻806)、F60702 (V6/T022//镇稻88)进行分子标记检测和田间条纹叶枯病抗性鉴定,其结果的符合率分别为99.3%、87.7%和91.8%。表明这些分子标记可以用于条纹叶枯病抗性基因Stv-bi分子标记辅助选择。 相似文献