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Optimal conditions for assaying hemolytic complement of goat (caprine) and swine (porcine) sera were determined. Effects of the following were tested: pH, ionic strength, calcium and magnesium ion concentrations, time and temperature of incubation, and ethylenediamine tetracetate concentration. Guinea pig erythrocytes sensitized with goat or cattle antibodies were the most sensitive target cells for goat complement. Sheep and cattle erythrocytes sensitized with rabbit hemolysin were the best target cells for swine complement. Barbital buffer, pH 7.3, ionic strength of 90 nmM relative salt concentration, containing 0.5 mM CaCl2 and 1 mM MgCl2 was the best for swine complement assay. Goat complement lysed best in a barbital buffer, pH 8, ionic strength of 90 to 120 mM of relative salt concentration, in presence of 0.5 mM CaCl2 and 1 mM MgCl2. The optimal incubation temperature was 37 degrees C for both complements. The complement dependent lysis required 75 minutes to reach its maximum. Ethylenediamine tetracetate in 4 mM concentration completely inhibited lysis by both species complements. The CH50 for goat sera varied between 18 and 75 per ml, in swine sera between 75 and 210 per ml. 相似文献
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C.D. Buxton 《African Zoology》2013,48(3):212-216
A histological study of the gonads of two sparid fishes, Chrysoblephus laticeps and C. cristiceps showed that these species are protogynous hermaphrodites. Both species are also monandric in which all males are derived from functional females. Intersexual individuals were restricted to a narrow size range between females and males, and no evidence for simultaneous hermaphroditism was found. Similar to other sparids, the development of the ovary and testis was delimited by a connective tissue layer. 相似文献
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抗CP型、NCP型牛病毒性腹泻病毒高免卵黄抗体的制备 总被引：1，自引：0，他引：1
本研究采用致细胞病变（cytopathic,CP）型牛病毒性腹泻病毒（bovine viral diarrhea virus,BVDV）标准毒和非致细胞病变（noncytopathic,NCP）型新疆优势毒株免疫产蛋鸡,用改良PEG法提取卵黄抗体(IgY),并对提取的IgY采用SDS-PAGE检测纯度,间接ELISA检测免疫后每隔7 d的抗体效价,并测定所得抗体对NCP型BVDV的中和效价。结果表明,用该法提取的IgY纯度较高;间接ELISA结果证明,经过4次免疫后,抗CP型BVDV的效价达到1∶32000,抗NCP型BVDV的效价达到1∶40000,3个月后再次检测,卵黄抗体效价未见明显下降。最后一次免疫14 d的抗体对NCP型BVDV的中和效价达到1×10-3。 相似文献
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Some kinetic data on the inactivation of equine herpesvirus type 1 (EHV1) and equine rhinovirus type 1 (ERhV1) by betapropiolactone (BPL) and ultraviolet (UV) irradiation are reported. 0.25% BPL at 37 degrees C for 1 h reduced the titre of EHV1 by greater than 10(3 . 4) and of ERhV1 by greater than 10(4 . 1) TCID50/ml. UV irradiation (334 microW/cm2) produced similar reductions in titre after 2 min. These data were used as a basis for inactivating EHV1 and ERhV1 by the combined action of BPL and UV irradiation. Viruses were exposed to 0.1% BPL for 1 h at 4 degrees C with constant stirring, followed by UV irradiation for 2 min, followed by incubation for 3 h at 37 degrees C. Inactivated EHV1 elicited secondary immune responses only in horses whereas ERhV1 produced primary immune responses in mice (including athymic nu/nu mice), rabbits and probably in horses. 相似文献
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P R Joner 《Acta veterinaria Scandinavica》1977,18(2):187-193
The antioxydants butylhydroxyanisol (BHA), butylhydroxytoluene (BHT) and ethoxyquin (EMQ) were tested for mutagenicity in the so-called “Ames test”. The test was performed with and without the addition of microsomal mixed function oxidase system from rat liver. The compounds were found not to be mutagenic in this test. At high concentration of BHA and EMQ toxic effects on the growth of the bacteria were observed. 相似文献
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Amphistomes of Calicophoron microbothrioides were recovered from the rumen of a Malaysian sambar deer (Cervus unicolor). Some measurements and illustrations of C. microbothrioides are given. This is a new host record for the species C. microbothrioides. 相似文献
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de Carvalho MA Santos PS Nogueira SS Lessa F Almeida Vdos A Le?a-Júnior NF Salvarani FM Lobato FC Nogueira-Filho SL 《Journal of zoo and wildlife medicine》2011,42(4):732-734
An outbreak of necrotic enteritis caused by Clostridium perfringens type C was diagnosed in captive collared (Pecari tajacu) and white-lipped (Tayassu pecari) peccaries housed in the Laboratory of Applied Ethology of Universidade Estadual de Santa Cruz located in Ilhéus, State of Bahia, Brazil. Four collared peccaries and three white-lipped peccaries, all juveniles (25-105 days old), were affected. For all affected animals, lethargy and inappetance were followed by sudden death within 24 hours. Histopathology of intestinal wall, culture of C. perfringens type C, and the identification of beta-toxin from intestinal content confirmed the diagnosis. 相似文献
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本文介绍了FSH及其受体的结构,重点讨论了雌性哺乳动物FSH的功能及FSH受体在卵巢中的表达。为进一步的研究工作提供了参考资料。 相似文献
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近年来,随着对乳铁蛋白和乳铁蛋白肽作用机制和空间结构的研究趋于成熟,国内外研究者逐渐将研究重点倾向于乳铁蛋白和乳铁蛋白肽的开发应用.主要介绍了乳铁蛋白和乳铁蛋白肽基因工程的研究进展,尤其是对原核表达系统和真核表达系统进行了综述. 相似文献