共查询到20条相似文献，搜索用时 156 毫秒
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基质金属蛋白酶(MMPs)是一类锌依赖的内源蛋白酶,能降解几乎所有的细胞外基质。研究发现,寄生虫存在MMPs,且感染宿主后可引起宿主MMPs表达改变,从而破坏宿主组织屏障,利于寄生虫入侵,调节寄生虫感染后疾病的发展与转归等。鉴于MMPs在寄生虫疾病中的重要地位,本文重点综述了寄生虫疾病中宿主和寄生虫各自MMPs的研究现状,为寄生虫疾病中基质金属蛋白酶功能的系统性研究提供参考。 相似文献
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虾青素金属蛋白酶是一种广泛存在于多种动物体内的金属蛋白酶，研究表明该蛋白酶在寄生线虫的入侵和生长发育过程中发挥重要作用。但目前对粪类圆线虫虾青素金属蛋白酶(Ss-AST-1)还所知甚少，研究旨在对该酶编码基因(Ss-ast-1)进行鉴定和表达分析，为进一步研究其功能奠定基础。利用生物信息学方法对Ss-ast-1基因进行序列分析，通过转录组数据分析Ss-ast-1在各个发育阶段的转录水平，转基因技术进行虫体内表达定位分析。通过体外原核表达重组蛋白，Western blot分析其抗原性。采用免疫荧光组织化学方法对Ss-AST-1在粪类圆线虫幼虫体内的定位进行研究。结果显示：Ss-ast-1基因全长1 905 bp,编码634个氨基酸；转录组分析该基因在iL3期的表达水平最高。定位分析表明该蛋白在虫卵细胞的细胞核和细胞质均有表达；体外表达重组蛋白的大小约67.5 kDa, Western blot分析表明多克隆抗体可以较好的识别全虫蛋白中的Ss-AST-1。免疫荧光结果显示Ss-AST-1在幼虫体内表达并分布幼虫的头部和体壁。Ss-ast-1属于虾青素金属蛋白酶家族，其可能在寄生虫入侵过程中... 相似文献
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半胱氨酸蛋白酶(CP)是广泛存在于动物和植物以及寄生虫中的一类含有半胱氨酸残基的蛋白水解酶,是蛋白酶家族中最重要的成员之一.目前,对人CP的研究宅要集中在凋亡和肿瘤两个方面,而对寄生虫CP的研究则相对比较广泛. 相似文献
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寄生虫半胱氨酸蛋白酶核酸疫苗的研究进展与应用前景 总被引：1，自引：1，他引：0
就寄生虫半胱氨酸蛋白酶的一些生理生化与免疫学性质进行了概括,阐述了该酶与宿主免疫应答之间的作用关系,证明了该酶具有作为研制高效核酸疫苗的条件,同时综述了寄生虫半胱氨酸蛋白酶核酸疫苗的发展以及应用前景。 相似文献
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从顶质体的发现、起源、形态学、基因组以及生物学功能等方面介绍了顶复门寄生虫顶质体的一些研究进展，说明了顶质体对于顶复门寄生虫的存活具有十分重要的作用，它参与了脂肪酸、血红素以及类异戊二烯的生物学合成。提示，顶质体所具有的新陈代谢功能也使其成为研制抗寄生虫药的潜在靶位点。 相似文献
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蜱是以吸血为生的体外寄生虫,能传播多种病原体。控制蜱的传统方法是使用杀虫剂,但会产生药物残留等问题。为了找到控制蜱及蜱传病的新方法,必须在分子水平上了解蜱的相关生理过程。蜱半胱氨酸蛋白酶抑制分子是一类抗蛋白酶分子,主要作用于半胱氨酸蛋白酶的抑制调控。论文介绍了蜱半胱氨酸蛋白酶抑制分子的结构和分类,以及在蜱的吸血、血液消化、蜱的先天免疫及对宿主免疫调节等生理过程的重要作用,同时也综述了半胱氨酸蛋白酶抑制分子具有抗寄生虫和抗癌的作用,为全面了解蜱半胱氨酸蛋白酶抑制分子提供参考。 相似文献
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寄生虫中蛋白酶的研究 总被引：1，自引：1，他引：0
寄生虫中蛋白酶的研究钟淑梅审核：沈杰（中国农科院上海家畜寄生虫病研究所）蛋白酶是催化蛋白质肽链水解的酶类，从病毒到人类普遍存在于整个生物界。生物体内的蛋白酶催化许多重要的生化反应，如激素前体的加工、血液凝固和血纤维蛋白水解作用、蛋白质的代谢、免疫反应... 相似文献
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范美红 《国外畜牧学(猪与禽)》2022,42(2):76-79
《生物化学杂志》(Journal of Biological Chemistry,JBC)一直是传播和记录蛋白酶发现和特性的主要载体。1971-2010年，在HerbTabor博士担任JBC主编期间，蛋白酶领域的研究取得了快速的发展。当他开始任职时，只有少数蛋白酶的精细结构和动力学为人所知。现在，人们已对数千种蛋白酶进行了鉴定，在人类基因组中发现了600多个蛋白酶基因。本综述除了回顾Herb Tabor博士对JBC以及美国生物化学和分子生物学学会(American society for biochemistry and molecular biology,ASBMB)在蛋白酶研究中所起的可贵贡献外，笔者还尽力概述了蛋白酶领域的研究历史，重点介绍了在蛋白酶研究过程中的一些发现及其在动物体内的作用。此外，本综述还讨论了金属蛋白酶，特别是龙虾肽酶家族中穿膜肽酶(meprin)的结构特征、调控、作用机制以及在动物健康和疾病中的作用。蛋白酶和蛋白质降解作用在生命系统中起着至关重要的作用，笔者还简要介绍了这一高度多样化和蓬勃发展的研究领域的未来方向。 相似文献
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C Sugimoto S Onoe W Z Zhuang M Onuma 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):931-936
The lysate of Theileria sergenti piroplasms was tested for proteinases using sodium dodecyl sulfate-polyacrylamide gel electrophoresis in which substrate was included in gel matrix. Six proteinases of molecular weight 330, 125, 98, 94, 67 and 58 kilodalton (kDa) were detected. From the results of the Triton X-114 phase partition, 330, 125 and 58 kDa proteinases were partitioned into aqueous phase, which indicated that they were not associated with parasite membranes. All these three enzymes were classified into metalloproteinase family because of their sensitivities to metal-ion chelating compounds, ethylenediaminetetraacetic acid (EDTA) and 1,10-phenanthroline. On the other hand, 98 and 94 kDa proteinases were membrane-associated metalloproteinases which were preferentially inhibited by 1,10-phenanthroline. Another metalloproteinase of 67 kDa which was inhibited by EDTA and 1,10-phenanthroline was not associated with parasite membranes. Proteinases of 98 and 94 kDa degraded heat-denatured hemoglobin. 相似文献
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Susan J. Kutz Emily J. JenkinsAlasdair M. Veitch Julie DucrocqLydden Polley Brett ElkinStephane Lair 《Veterinary parasitology》2009
Climate change is influencing the structure and function of natural ecosystems around the world, including host–parasite interactions and disease emergence. Understanding the influence of climate change on infectious disease at temperate and tropical latitudes can be challenging because of numerous complicating biological, social, and political factors. Arctic and Subarctic regions may be particularly good models for unraveling the impacts of climate change on parasite ecology because they are relatively simple systems with low biological diversity and few other complicating anthropogenic factors. We examine some changing dynamics of host–parasite interactions at high latitudes and use these to illustrate a framework for approaching understanding, preventing, and mitigating climate change impacts on infectious disease, including zoonoses, in wildlife. 相似文献
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Leibman NF Lana SE Hansen RA Powers BE Fettman MJ Withrow SJ Ogilvie GK 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(6):583-586
Presence of matrix metalloproteinases has been associated with tumor invasion and metastasis in human neoplasia. The presence of matrix metalloproteinase 2 and matrix metalloproteinase 9 was determined in canine mast cell tumor tissue and normal stromal tissue from 24 dogs with spontaneously occurring cutaneous mast cell tumors. Seventeen of the mast cell tumors were of histologic grade 2, and 7 were of histologic grade 3. Gelatin zymography and computer assisted densitometry image analysis were used to quantify matrix metalloproteinase concentration. Bands from canine tissues migrated in the same location as human proenzyme and active enzyme matrix metalloproteinase 2 and matrix metalloproteinase 9 standards. A semiquantitative value for each patient sample was obtained by comparing the optical assessment density of each unknown band to the optical density of the human standard. The presence of matrix metalloproteinase 2 and matrix metalloproteinase 9 in histologic grade 2 mast cell tumors and histologic grade 3 mast cell tumors was compared, as was presence of matrix metalloproteinases in tumor and stromal tissue. There was dramatically more proenzyme matrix metalloproteinase 9 activity in histologic grade 3 mast cell tumors when compared to grade 2 tumors (P = .03). There was also dramatically more active enzyme matrix metalloproteinase 2 and active enzyme matrix metalloproteinase 9 activity in tumor tissue compared to stromal tissue (P = .02, P < .0001). This study demonstrates that the proenzyme and active enzyme forms of matrix metalloproteinase 2 and matrix metalloproteinase 9 are present in canine mast cell tumors. This appears to be related to the degree of histologic malignancy, although histologic grade 1 tumors were not evaluated. 相似文献
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转录组测序(RNA-seq)是通过高通量测序方法全面快速的获悉特定组织或细胞的特定发育阶段或功能状态下所有RNA序列信息的技术。该技术以其准确和高通量等特征,被广泛应用于生物学、医学和农学等基础性研究。寄生虫在体外发育、感染入侵和体内繁殖等不同阶段,发生着一系列复杂的生物变化。采用RNA-seq技术筛选寄生虫发育、感染和繁殖等不同阶段的差异表达基因,阐明其功能,获悉寄生虫-宿主互作的分子调控机制等研究是目前的研究重点和热点。基于此,作者对RNA-seq技术在寄生虫研究中的应用作一综述。 相似文献
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Managing anthelmintic resistance: untreated adult ewes as a source of unselected parasites, and their role in reducing parasite populations 总被引：1，自引：0，他引：1
Leathwick DM Miller CM Atkinson DS Haack NA Waghorn TS Oliver AM 《New Zealand veterinary journal》2008,56(4):184-195
AIMS: To test the hypotheses that when untreated adult ewes are rotationally grazed (follow behind) on pastures after lambs receiving routine anthelmintic treatments, the ewes can function as a source of unselected parasites in refugia, capable of slowing the development of anthelmintic resistance, and suppress the build-up of parasites resulting from the development of anthelmintic resistance. METHODS: Firstly, the potential of untreated adult ewes to slow the development of anthelmintic resistance, and to suppress parasite populations under differing levels of anthelmintic efficacy, was investigated using a simulation model. Secondly, a field trial with three replicates of each treatment compared two grazing systems (lambs only vs lambs followed by ewes) and two types of anthelmintic, viz albendazole (ALB), to which resistance was present (faecal nematode egg count reduction (FECR)=57-59%) and ivermectin plus levamisole (IL), to which resistance was absent (FECR=97-99%), in a factorial treatment structure. Parasite populations were monitored using faecal nematode egg counts (FEC), faecal larval cultures, pasture larval sampling, and slaughter of tracer lambs. Animal performance was measured using liveweight, dag score, body condition score, and fleece weights. RESULTS: Model simulations indicated that parasites cycling in the untreated ewes could slow the development of resistance being selected for by the anthelmintic treatments given to lambs and this could occur without a nett increase in larval numbers on pasture. Further, as worm control in the lambs declined with increasing levels of anthelmintic resistance the ewes increasingly functioned as nett removers of parasite larvae, effectively reducing parasite population size. In the field trial, untreated adult ewes contributed to pasture infestations of most parasite species, but not Nematodirus spp. Parasite species on pasture and infecting lambs changed when ewes were present, but larval populations on pasture in the autumn were no greater than when lambs grazed alone. In the presence of anthelmintic resistance, parasite populations were reduced when ewes grazed in rotation with lambs, implicating the ewes as nett removers of parasite challenge. CONCLUSIONS: Untreated adult ewes were a source of unselected genotypes, capable of slowing the development of anthelmintic resistance in most, but not all, parasite species. Further, the potential of adult ewes to remove from pasture more parasite larvae than they contribute through faecal contamination indicates a potentially useful role in suppressing parasite populations, particularly when worm control in lambs is less effective as a result of anthelmintic resistance. 相似文献
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寄生虫在入侵机体时必须具备逃避机体免疫,破坏宿主免疫屏障才能感染,这种入侵过程必然和宿主机体的免疫系统发生联系,不但使宿主的先天免疫细胞功能缺失,而且获得性免疫也受到抑制。寄生虫感染均不同程度地伴随免疫损伤,宿主在感染寄生虫后产生的免疫应答中,细胞因子作为免疫过程中的介质起到关键作用,主要是调节机体细胞免疫抗虫,保护机体不被感染,其中巨噬细胞是主要功能的承担者。本文将阐述细胞因子在寄生虫感染过程中所发挥的免疫作用,为解决寄生虫感染的防控问题奠定基础,同时为寄生虫的研究提供新的思路。 相似文献
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Aupperle H Thielebein J Kiefer B März I Dinges G Schoon HA 《Veterinary journal (London, England : 1997)》2009,180(1):88-94
While the pathogenesis of chronic valvular disease (CVD) in dogs remains unclear, alterations in the activity of specific metalloproteinase enzymes and their inhibitors within the valve stroma are suspected of having a role. This study describes the immunohistochemical distribution pattern of matrix metalloproteinase (MMP) types 2, 9 and 14 and their tissue inhibitors, termed tissue inhibitors of metalloproteinase (TIMP), types 2 and 3, in normal canine mitral valves (MVs) (n=10) and in dogs with mild (n=7), moderate (n=14) and severe (n=9) CVD. In normal MVs, MMP-2 and -14, and TIMP-2 were expressed in isolated stromal cells. Tissue inhibitor of metalloproteinase-3 exhibited moderate intracellular and mild extracellular expression. With increasing severity of CVD, the expression of MMP-2 decreased. The number of stromal cells expressing MMP-14 increased, predominantly in the margins of the nodular lesions. Tissue inhibitor of metalloproteinase-2 and -3 expression increased both intra- and extracellularly. Matrix metalloproteinase-9 was not detected in normal or diseased valves. In conclusion, CVD was characterised by alterations in the distribution and intensity of valvular MMP and TIMP expression, suggesting that depressed catabolism and the accumulation of extracellular matrix components within affected valves contributes to their structural alteration and consequent loss of function. 相似文献