共查询到20条相似文献,搜索用时 984 毫秒
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[目的]调查江苏省泰州市某鹅场鹅大批死亡的原因。[方法]通过流行病学调查、临床诊断、病理变化、动物回归感染试验、血清学检查等对江苏省泰州市某鹅场发生的疑似鹅副黏病毒病的病料进行病原分离与鉴定。[结果]该病毒能使SPF鸡胚以及10日龄鹅和鸡全部死亡,能凝集鸡红细胞并能被NDV标准阳性血清所抑制,不能被禽流感病毒标准阳性血清所抑制。发病死亡与人工感染死亡的临诊症状和病理变化基本一致。经电镜观察发现该病毒颗粒呈圆形,直径在150 nm左右,有囊膜及纤突。该分离毒株经灭活后免疫鹅群,取到良好的防治效果。[结论]该病毒为禽Ⅰ型副黏病毒,属副黏病毒科副黏病毒属。 相似文献
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采用9日龄鸡胚,从长春某个体养鹅户的病死鹅的肝、脾和肾脏中分离到1株病毒,暂定名为JG04株。经HA和HI试验、电镜观察、常规RT-PCR、NDV荧光RT-PCR检测,证实JG04株为鹅源副黏病毒。与GenBank下载的9株NDV毒株的HN基因作同源性比较,并进一步对JG04株进行耐酸试验、氯仿和乙醚敏感试验、血凝解脱及血凝素热稳定性试验、EID50、ELD50和毒力测定。结果表明:JG04株与江苏近年来分离的鹅源副黏病毒株的HN基因同源性较高,核苷酸同源性高达96.4%,氨基酸同源性高达96.9%;JG04株有良好的血凝性,能凝集人和鸡的红细胞;电镜观察病毒粒子有囊膜,直径约200 nm;对酸、氯仿、乙醚敏感;血凝解脱为快速解脱型,血凝素热稳定性差;EID50、ELD50分别为1.74×109/mL、2.57×109/mL;MDT为54.2 h,ICPI为1.66,IVPI为2.60。上述结果表明,GJ04株与新城疫病毒(NDV)速发型具有类似的毒力,属强毒力毒株。 相似文献
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利用SPF鸡胚培养的方法对上海郊区疑似发生鸽副黏病毒I型感染的鸽场病料进行病毒分离,病料上清液在10~11日龄SPF鸡胚上盲传5代,鸡胚死亡时间趋于规律性,鸡胚在接种72h内死亡90%以上,死亡胚全身出血。通过对病毒液进行病原学鉴定(该病毒液具有HA,这种血凝性可被NDV参考阳性血清抑制,而不能被EDSV、AIV—H5、AIV—H9、AIV—H7阳性血清抑制)、致病指数(采用SPF鸡胚和SPF鸡测定致病指数分别为MDT=64.8h、ICPI=1.3625、IVPI=0.75)及鸽体致病性等试验,初步鉴定为鸽副黏病毒I型中等毒力毒株,命名为鸽副黏病毒I型(S—1株)。 相似文献
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水禽源禽1型副黏病毒强毒RT-PCR方法的建立 总被引:1,自引:0,他引:1
参照GenBank上登录的水禽源禽1型副黏病毒强毒株F基因序列设计引物,建立检测水禽源禽1型副黏病毒强毒株的RT-PCR方法.该方法能从水禽源禽1型副黏病毒强毒株扩增出1条400 bp的特异片段,从水禽源禽1型副黏病毒弱毒、鸭瘟病毒、鸭1型肝炎病毒、减蛋综合征病毒、传染性法氏囊病病毒、H9亚型禽流感病毒和禽多杀性巴氏杆菌等均不能扩增出目的片段.敏感性试验显示该RT-PCR方法最低可检测出10 pg的病毒核酸.因此,该RT-PCR方法可用于水禽源禽1型副黏病毒强毒的临床诊断. 相似文献
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为了调查蛋鸭禽1型副黏病毒的感染状况,以RT-PCR技术对2011年以来采自福建、浙江、江西、广东、广西和江苏六省(区)的495份蛋鸭样品进行了禽1型副黏病毒的检测,并测定了部分禽1型副黏病毒分离株对不同日龄麻鸭的致病性。结果表明六省(区)份蛋鸭的禽1型副黏病毒的阳性感染率高低不一,为3.4%~10.7%,揭示了禽1型副黏病毒在我国蛋鸭中存在不同程度的感染;禽1型副黏病毒感染可致死低日龄麻鸭,其中基因VII型鸭源分离株对麻鸭的致死率高于基因IX型,且随着麻鸭日龄的增长其致死率降低,即呈现日龄易感性差异,开产麻鸭感染禽1型副黏病毒则表现为产蛋率显著下降。 相似文献
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禽流感由正黏病毒科流感病毒属A型流感病毒引起;新城疫由副黏病毒科副黏病毒亚科腮腺炎病毒属的禽副黏病毒I型引起。目前我国对禽流感和新城疫的预防主要采取强制免疫、加强消毒、隔离、无害化处理等多项防疫措施,定期进行血清学与病原学的主动监测,强化补免,进而形成高水平的免疫抗体屏障。 相似文献
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Origins of molecular biology 总被引:1,自引:0,他引:1
E L Hess 《Science (New York, N.Y.)》1970,168(932):664-669
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We report the incrementally staged design, synthesis, characterization, and operation of a molecular machine that behaves like a nanoscale elevator. The operation of this device, which is made of a platformlike component interlocked with a trifurcated riglike component and is only 3.5 nanometers by 2.5 nanometers in size, relies on the integration of several structural and functional molecular subunits. This molecular elevator is considerably more complex and better organized than previously reported artificial molecular machines. It exhibits a clear-cut on-off reversible behavior, and it could develop forces up to around 200 piconewtons. 相似文献
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Computer-aided molecular design 总被引:6,自引:0,他引:6
J A McCammon 《Science (New York, N.Y.)》1987,238(4826):486-491
Theoretical chemistry, as implemented on fast computers, is beginning to yield accurate predictions of the thermodynamic and kinetic properties of large molecular assemblies. In addition to providing detailed insights into the origins of molecular activity, theoretical calculations can be used to design new molecules with specific properties. This article describes two types of calculations that show special promise as design tools, the thermodynamic cycle-perturbation method and the Brownian reactive dynamics method. These methods can be applied to calculate equilibrium and rate constants that describe many aspects of molecular recognition, stability, and reactivity. 相似文献
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Bally J 《Science (New York, N.Y.)》1986,232(4747):185-193
The interstellar medium in our galaxy contains matter in a variety of states ranging from hot plasma to cold and dusty molecular gas. The molecular phase consists of giant clouds, which are the largest gravitationally bound objects in the galaxy, the primary reservoir of material for the ongoing birth of new stars, and the medium regulating the evolution of galactic disks. 相似文献
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The molecular genetics of cancer 总被引:87,自引:0,他引:87
J M Bishop 《Science (New York, N.Y.)》1987,235(4786):305-311
The search for genetic damage in neoplastic cells now occupies a central place in cancer research. Diverse examples of such damage are in hand, and they in turn hint at biochemical explanations for neoplastic growth. The way may be open to solve the riddles of how normal cells govern their replication and why cancer cells do not. 相似文献
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