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1.
Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 microg/ml and MBC in the range of 256-512 microg/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth.  相似文献   

2.
The methanolic extract of Verbascum sinuatum inflorescences and isolated compounds were tested for their antibacterial activity against 13 Gram(+) and Gram(-) bacteria and for their allelopathic activity against radish.  相似文献   

3.
外来树种火炬树化感作用的研究   总被引:1,自引:1,他引:0       下载免费PDF全文
以盐肤木和黄栌为参照树种,用火炬树树叶水浸提液、林下土壤水浸提液浸种24 h,研究了浸提液对侧柏、油松、香椿、臭椿、小麦、小白菜种子萌发及幼苗生长的影响,研究了火炬树的化感作用。结果表明:火炬树完整鲜叶水浸提液处理小白菜种子,其发芽率和发芽势分别为88.0%、82.5%,与对照发芽率(94.0%)和发芽势(91.5%)无显著差异,而破碎鲜叶水浸液则极显著影响小白菜的发芽率和发芽势,分别降低为24.8%和5.0%;火炬树完整鲜叶和破碎鲜叶水浸液都能促进小白菜苗高生长,但都抑制小白菜胚根的伸长。与鲜叶相比,火炬树干叶水浸提液的化感作用明显降低。火炬树破碎鲜叶水浸提液极显著影响小麦发芽率,分别比对照的发芽率和发芽势降低11.0%和83.5%,干叶水浸提液则不影响小麦的发芽率,为95.0%,但发芽势比对照降低23.0%。与盐肤木和黄栌相比,火炬树破碎鲜叶水浸提液对小麦幼茎及胚根生长的抑制作用最强。火炬树破碎鲜叶水浸提液对4个乡土树种的种子萌发产生了不同程度的抑制作用,对香椿及臭椿发芽率的抑制作用极显著。火炬树破碎鲜叶水浸提液对4个树种的苗高以及油松苗胚根的伸长没有显著影响,但却极显著地抑制了侧柏、香椿及臭椿胚根的伸长。火炬树林下表层土壤水浸提液对小麦幼苗的生长没有抑制作用,能明极显著促进香椿的生长;处理55 d时,香椿苗平均高为8.63 cm,极其显著高于对照(5.14 cm)。  相似文献   

4.
飞机草水提液对任豆种子萌发和幼苗生长的影响   总被引:1,自引:0,他引:1  
以任豆为受体植物,研究了飞机草根、茎和叶的水提液对任豆种子萌发和幼苗生长的化感效应。结果表明,不同浓度水提液对任豆的化感作用强度不同,在100%高浓度下才能明显延缓任豆种子发芽速率和显著抑制幼苗根的生长;在不同部位水提液中,叶水提液的化感作用最强,且表现出低促高抑的现象;而各部位、各浓度的飞机草水提液对任豆的发芽率、苗高和干重等重要指标均无显著抑制作用,表明任豆对飞机草的化感作用具有较强抵抗力。  相似文献   

5.
研究了长白落叶松未分解和半分解枯落针叶的水浸液处理对红皮云杉种子和长白落叶松种子萌发的不同生物效应。对于长白落叶松种子萌发来说,长白落叶松半分解和未分解枯落针叶水浸液4.0 g/L浓度的处理效果均为最佳;而对于红皮云杉种子萌发来说,10.0 g/L浓度或0.5~1.0 g/L浓度的处理效果为最佳。长白落叶松枯落针叶水浸液处理对长白落叶松自身种子和对红皮云杉种子萌发的作用均有很大的差异,推测这是长白落叶松枯落针叶水浸液中所含的化感物质和营养物质与长白落叶松和红皮云杉萌发过程中种子产生的不同代谢物质之间不同相互作用的反映。  相似文献   

6.
毛竹叶及其竹鞭生化物质对杉木种子的发芽效应   总被引:1,自引:0,他引:1  
通过杉木种子发芽试验,检测毛竹叶及竹鞭生化物质对杉木的化感作用;就总体而言,毛竹叶和竹鞭生化物质对杉木种子发芽有着一定程度的促进作用;毛竹叶及竹鞭非极性与极性生化物质的差异而言,毛竹叶及鞭弱极性生化物质对杉木种子发芽的促进作用强于极性生化物质。  相似文献   

7.
Agroforestry is a leading alternative for food security and forest conservation. A full understanding of positive and negative, i.e. allelopathic, interactions between crops and forest trees is necessary for producing crops and conserving forests especially within the threatened Mediterranean forest ecosystems. The present study explored the allelopathic effects of green and senescent leaf and soil extracts of two agroforestry trees—Pinus halepensis and Quercus coccifera—on the germination of wheat, barley, lentil, chickpea, and fababean as the major grain crops of Jordan. Results revealed that allelopathic effects reduced seed germination of these crops. Germination reduction reached a maximum of 75% in fababean treated with green extracts of Q. coccifera and differed among crops and extract sources, but not between tree species. Comparing between green and senescent leaf and soil extract, regarding their effect on germination percentage, it was noticed that these effects were similar in some crops and were different in others. Germination responses were generally different between cereals and legumes where cereals tend to be less affected by allelopathic influences than legumes, especially fababean. We suggest using cereals such as wheat and barley in agroforestry practices in the Mediterranean region of Jordan.  相似文献   

8.
采用正交试验及超临界CO_2流体萃取技术萃取杉木根化感物质,并通过杉木种子发芽试验进行杉木根化感物质对杉木的化感作用进行评价。结果表明:就化感物质萃取得率而言,在众多萃取因素当中,萃取压力和萃取温度是两个最主要的因素;经纯CO2及乙醇和CO2混和萃取的杉木根化感物质对杉木存在不同的化感效应,其中经乙醇和CO2混和萃取的杉木根化感物质对杉木种子发芽的抑制效应强于纯CO2萃取的化感物质。表3参20。  相似文献   

9.
野扇花果肉和胚乳发芽抑制物的研究   总被引:1,自引:0,他引:1  
通过采用野扇花果肉和胚乳的水浸提液对白菜种子进行发芽试验,结果表明:果肉水浸提液高浓度时显著抑制白菜种子的萌发,但低浓度时反而促进种子的萌发;胚乳水浸提液对白菜种子的萌发没有显著影响。2种水浸提液均对幼苗的根和子叶的伸展有一定的抑制作用。  相似文献   

10.
Gbolade AA  Adeyemi AA 《Fitoterapia》2008,79(3):220-222
Extracts from Pycnanthus angolensis stem bark, and fruits and seeds of Sphenocentrum jollyanum, when tested in vitro, showed potent anthelmintic activity on the earthworm, Eudrilus eugeniae. Methanolic extract of P. angolensis was more active than its chloroform extract (P<0.001), while fruit ethanolic extract of S. jollyanum was also more potent than the seed extract. When compared with other worms, S. jollyanum fruit extract showed the greatest activity on wireworms when tested at 80 mg/ml.  相似文献   

11.
Kwon DY  Kang OH  Choi JG  Lee YS  Oh YC  Chae HS  Lee GH  Park PS  Kim YC  Sohn DH  Park H  Lee JH 《Fitoterapia》2007,78(6):430-433
Methanol extract and its fractions (hexane, EtOAc, n-BuOH, and H2O) of Dryopteris crassirhizoma were investigated for antibacterial activity against methicillin-resistant Staphylococcus aureus. The hexane fraction showed a good antibacterial activity against all tested strains.  相似文献   

12.
卷叶黄精根不同极性化感物质的化感作用   总被引:1,自引:0,他引:1  
王冬梅  李登武  朱玮  李娟丽 《林业科学》2007,43(10):145-149
化感作用是植物通过淋溶、挥发、残茬降解和根系分泌向环境中释放化学物质,从而对自身或周围其他植物的生长产生影响的现象(孔垂华等,2001;董卓杭等,2001;翟明普等,1993;林文雄等,2001;马茂华等,1999;彭少麟等,2001).化感作用普遍存在于自然界,对其深入研究将有利于复合系统中植物配置、耕作制度和栽培措施的科学化,在植物间加强促进作用,降低抑制作用,促进生物多样性和农业可持续发展.  相似文献   

13.
The methanol extract of the dried leaves of Abies webbiana was evaluated for antimicrobial activity. The methanol extract showed a broad spectrum of antibacterial activity.  相似文献   

14.
E Attard  H Attard 《Fitoterapia》2008,79(3):226-228
The antitrypsin activity of extracts obtained from two different seed types of Ecballium elaterium, was tested in vitro. The presence of trypsin inhibitors in three chromatographed samples for each extract, showed a strong and specific antitrypsin activity.  相似文献   

15.
以水浸提法提取薄壳山核桃和山核桃2种植物的叶片内含物,研究其对刺槐、萝卜和黑麦草种子萌发和幼苗生长的化感作用。结果表明:水浸提物明显抑制了3种受试植物种子的萌发及其幼苗的生长;生长7 d的幼苗体内,可溶性蛋白、SOD活性、POD活性降低,MDA含量升高。通过氯仿热回流及高效液相色谱法,提取、鉴定了2种植物叶片中的化感物质胡桃醌,并测定其含量。结果表明,薄壳山核桃叶片中所含有的胡桃醌含量高于山核桃。  相似文献   

16.
Sasikumar JM  Doss PA 《Fitoterapia》2006,77(7-8):605-607
Methanol extract of Rhaphidophora pertusa stem was analyzed for its antioxidant (DPPH, reducing power and Fe(3+) metal chelation methods) and antibacterial activities. The extract was found effective against the three antioxidant test models and exhibited strong and moderate antibacterial activity against the tested pathogenic bacteria.  相似文献   

17.
Allelochemicals of Chinese-fir root was extracted by technology of supercritical CO2 extraction under orthogonal experiment design, and it was used to analyze allelopathic activity of Chinese-fir through bioassay of seed germination, The results showed that as to the available rate of allelochemicals, the pressure and temperature of extraction were the most im-portant factors, The allelochemicals of Chinese-fir root extracted by pure CO2 and ethanol mixed with CO2 have different al-lelopathic activities to seed germination, and the allelochemicals extracted by ethanol mixed with CO2 had stronger inhibitory effects on seed Qermination than that extracted by pure CO2.  相似文献   

18.
4种经济树种叶片浸提液对作物种子萌发化感作用的研究   总被引:1,自引:0,他引:1  
用广西产的马尾松、巨尾桉、香樟、油茶4种主要经济树种的鲜叶及凋落叶浸提液作浸泡萝卜、油青菜心及上海青种子处理,进行了其对作物种子萌发影响的化感作用研究。结果表明,在4个树种凋落叶的浸提液处理中,化感作用最强的为香樟,其次为油茶、巨尾桉,最弱的为马尾松;而在4个树种鲜叶的浸提液处理中,化感作用最强的为巨尾桉,其次为香樟、油茶,最弱的为马尾松。其中萝卜种子的萌发对香樟叶片的浸提液更为敏感,而上海青种子的萌发则对油茶叶片的浸提液更为敏感。  相似文献   

19.
桑叶总黄酮的提取及其抑菌活性研究   总被引:2,自引:0,他引:2  
以抑菌活性为评价指标,对桑叶总黄酮的提取工艺进行了优化,并对优化条件下得到的粗提物采用不同溶剂进行分级提取和定性分析,测定了各萃取物的总黄酮含量,考察了萃取物对大肠杆菌、金黄色葡萄球菌的抑菌圈直径、最低抑菌浓度,选取抑菌效果最佳的萃取物进行了稳定性实验。结果表明,桑叶总黄酮提取的最佳条件为:温度70℃,乙醇体积分数70%,提取时间4 h,固液比1∶30(g∶mL);抑菌效果最佳的为乙酸乙酯萃取物,且乙酸乙酯部分对温度和紫外线照射的影响均具有良好的稳定性。  相似文献   

20.
毛白杨他感作用的研究   总被引:4,自引:0,他引:4  
用毛白杨根系提取液处理大豆、玉米、小麦、花生和常见田间杂草的种子,进行发芽试验,研究毛白杨是否存在他感作用。结果表明:不同浓度的浸提液对几种农作物和杂草的种子萌发、幼苗生长、根生长均有不同程度的抑制或促进作用。  相似文献   

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