共查询到19条相似文献，搜索用时 234 毫秒
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亮菌多糖ATPS-2对小鼠四氯化碳和酒精肝损伤的保护作用 总被引：2，自引：0，他引：2
分别采用四氯化碳（CCl4）和北京红星二锅头诱导小鼠肝损伤，比色法测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）的含量；肝脏中超氧化物歧化酶（SOD）活力和丙二醛（MDA）浓度，并作肝组织切片病理观察。亮菌多糖ATPS-2（25mg／kg、50mg／kg、100mg／kg）给药明显降低小鼠血清中升高的ALT和AST水平，抑制肝脏中上升的MDA水平和提高过低的SOD活性。病理检查结果显示亮菌多糖ATPS-2有明显的保肝作用。亮菌多糖ATPS-2对小鼠四氯化碳肝损伤和酒精肝损伤均具有显著的保护作用。 相似文献
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测定了灵芝发酵产物和添加薏苡仁的灵芝发酵产物对小鼠抗氧化性。结果表明:灵芝和加薏苡仁后的灵芝能提高小鼠的器官指数和SOD、GSH-PX活性、降低组织中MDA、脑褐素的含量。添加薏苡仁后的灵芝中剂量组能提高血清肝组织中的SOD活性,而低剂量组能提高脑中的SOD活性;低剂量组能降低血清中MDA含量,而中剂量组能降低脑中MDA的含量;低剂量组能提高血清、肝的GSH-PX活性,但不能提高对脑中的GSH-PX活性;高剂量组能降低心脂褐素的含量,而中剂量组能降低脑脂褐素的含量。 相似文献
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裂褶菌对小鼠抗疲劳作用的研究 总被引：1，自引：0，他引：1
以裂褶菌子实体干粉（试验Ⅰ组和试验Ⅱ组）和裂褶菌发酵液（试验Ⅲ组）饲喂小鼠，检测裂褶菌子实体干粉和发酵液对小鼠肝糖原含量、肌糖原含量、琥珀酸脱氢酶（succinate dehydrogenase，SDH）活力、乳酸脱氢酶（lactic dehydrogenase，LDH）活力、血清尿素氮（blood urea nitrogen，BUN）含量及血乳酸（lactic acid，LD）含量的影响。结果表明，试验Ⅰ组、试验Ⅱ组和试验Ⅲ组小鼠的肝糖原含量、SDH活力及LDH活力极显著高于对照组（饲喂100％基础饲料）（p〈0．01）；试验Ⅰ组和试验Ⅱ组的肌糖原含量极显著高于对照组（P〈0．01）；运动后试验Ⅰ组、试验Ⅱ组和试验Ⅲ组小鼠的BUN增量和LD的水平极显著低于对照组（P〈0．01），说明裂褶菌具有明显解除运动疲劳的作用。 相似文献
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AIM: To study the effect of microRNA-7 (miR-7) knockdown (KD) on concanavalin A (ConA)-induced acute liver injury (ALI) in mice.METHODS: Wild type (WT) mice and miR-7KD mice were received ConA (30 mg/kg) to induced acute liver injury model by intraperitoneal injection, and the morphological changes, liver weight and weight index were measured 48 h later. The pathological changes of the liver tissues were observed by HE staining. The levels of serum alanine aminotransferase (ALT), IL-4 and IFN-γ were detected by ELISA. The proportional changes of CD4+ T cells and the relative levels of IL-4 and IFN-γ were analyzed by flow cytometry.RESULTS: The color of the liver tissue became lighter, and the weight and weight index were changed significantly in miR-7KD mice compared with control group (P<0.05). HE staining showed that the inflammatory cell infiltration was increased in the liver of miR-7KD mice. Moreover, the level of serum ALT was significantly increased (P<0.05). The serum level of IFN-γ elevated significantly (P<0.01), while the IL-4 levels decreased significantly (P<0.01) in the serum of miR-7KD mice. Furthermore, the proportion of CD4+ T cells and relative IFN-γ cells increased obviously (P<0.01).CONCLUSION: miR-7 knockdown promotes the pathogenesis of the ConA-induced acute liver injury in mice. 相似文献
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冬虫夏草菌丝体免疫特性测试 总被引：3，自引：0，他引：3
以发酵生产的冬虫夏草菌丝体为研究材料,通过测试其对荷瘤小鼠超氧化物歧化酶(SOD)和脂质过氧化物(LPO)水平的影响和观察小鼠组织细胞的病理变化,测试冬虫夏草的免疫功能。试验表明,冬虫夏草菌丝体能显著增加荷瘤小鼠脑组织和全血中SOD含量,从而提高机体免疫力,明显减少荷瘤小鼠S180癌细胞面积,降低癌细胞重量,促进癌细胞高度分化,致使癌细胞坏死,对肿瘤生长表现出较强的抑制力。 相似文献
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AIM: To observe the expression of undulin (Un) in liver tissue and to clarify the diagnostic significance of serum Un in experimental rat liver fibrosis. METHODS: Rat liver fibrosis was induced by carbon tetrachloride. The expression of Un in liver tissue was detected by immunohistochemical method. Serum Un levels was measured by enzyme immunoassay. RESULTS: Expression of Un increased in fibrotic liver than normal liver, and it was mainly distributed in portal tract stroma, central veins and fibrotic septa in fibrotic liver. Also, the level of serum Un was significantly higher in fibrotic liver than normal liver. CONCLUSION: These data suggest that Un should be a component of the hepatic extracellar matrix, and its expression could be increased greatly in fibrotic rat liver. Serum Un levels may be used as an indicator in liver fibrosis diagnosis. 相似文献
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AIM: To investigate the effect of recombinant human interleukin-10 (rhIL-10) on IL-6 and TNF-α levels in serum and liver of mice exposed to lipopolysaccharide (LPS). METHODS: rhIL-10 was prepared by using genetic engineering technology. Mice were intraperitoneally with 500 μg of LPS, and then were treated intravenously with various dosages of rhIL-10. The levels of IL-6 and TNF-α in hepatic tissue and serum were determined by ELISA at 12 h, 24 h, 48 h and 72 h post rhIL-10 treatment. RESULTS: rhIL-10 markedly inhibited the increase in IL-6 and TNF-α levels in hepatic tissue and serum at 12 h after rhIL-10 treatment in LPS-challenged mice, and the inhibition effect was significant at 24-48 h after rhIL-10 treatment （P＜0.05）. CONCLUSION: rhIL-10 can inhibit the increase in IL-6 and TNF-α levels induced by LPS in mice. 相似文献
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AIM: To study whether the pulmonary infection of Escherichia coli (E. coli) interferes the glucolipid metabolism in high-fat diet-induced obese mice. METHODS: High-fat diet-induced obese mice (n=48) and normal chow-fed control mice (n=48) were intranasally infused with 40 μL fluid containing 4×109 CFUs E. coli. The serum, periepididymal adipose tissue and liver were obtained at 0 d, 1 d, 2 d, 3 d and 4 d after infection. The body mass, periepididymal adipose tissue and liver were weighed, and the levels of fasting blood glucose (FBG), fasting blood insulin (FINS), free fatty acid (FFA) and very-low-density lipoprotein (VLDL) were measured by ELISA. The serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), and hepatic TG contents were detected, and the hepatic steatosis was observed under microscope with oil red O staining. RESULTS: Compared with day 0, the body mass, fat mass and fat index were decreased significantly from day 1 to day 4 after infection (P<0.05). The levels of FBG, FINS and HOMA-IR were apparently raised from day 2 to day 4 after infection (P<0.05). The contents of serum FFA, TG and VLDL were increased markedly from day 1 to day 4 after infection (P<0.05). However, the concentrations of serum TC, LDL-C and HDL-C were decreased obviously from day 1 to day 3 (P<0.05). The liver mass, liver index and TG content were significantly increased from day 1 to day 4 (P<0.05). Consistently, the lipid droplet accumulation in the liver cells was increased obviously at day 2 and day 4 after infection. Compared with control group, except the levels of serum TC, LDL-C and HDL-C in obese group substantially decreased, the other indexes were increased by different degrees during the whole experiment period (P<0.05). CONCLUSION: Pulmonary infection of Escherichia coli exacerbates the disorder of glucose and lipid metabolism in high-fat diet-induced obese mice, which contributes the development of insulin resistance and hepatic steatosis. 相似文献
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AIM: To study the effect of glycine on body weight and lipid metabolism in mice. METHODS: Mice were randomly divided into four groups, control, high-fat diet group (HF), glycine group (Gly), and glycine treatment group (Gly+HF). The mice were fed with normal diet and high-fat diet for 12 days, respectively, and then were treated with glycine solution by intraperitoneal injection. Finally, the serum was collected to measure serum lipid, epididymal fat-pads, pararenal fat-pads and abdominal fat-pads were taken out to weight. Moreover, pathologic changes in liver were observed. RESULTS: The body weight of HF group was obviously lower than that in control; the body weight and adipose tissue content of Gly group were obviously lower than those in control; the body weight and adipose tissue content of Gly+HF group were obviously lower than those in HF group; the levels of TC and TG in Gly group were obviously lower than those in control; there was no statistical difference in serum lipid between Gly+HF and HF groups. In addition, marked hepatic steatosis in HF group, and slight hepatic steatosis in Gly+HF group were found. CONCLUSION: Gly can decrease the body weight and adipose tissue content and serum lipid in normal diet and high-fat diet mice, and it also can prevent hepatic steatosis. 相似文献
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研究金针菇多糖对正常小鼠学习记忆的影响。利用三等分辐射式迷宫箱训练小鼠,检测每实验日正确反应率、达标小鼠的比率及达标所需训练次数,训练结束后取脑,用紫外分光光度计检测各项指标的OD值,测得超氧化物歧化酶（SOD）和丙二醛（MDA）含量。结果显示低剂量金针菇多糖可显著提高小鼠的达标率,显著降低小鼠达标的训练次数,金针菇多糖对SOD含量水平均有显著的提高作用（p〈0.05）,低剂量的金针菇多糖明显提高大脑海马组织中蛋白质的含量（p〈0.05）并能显著降低MDA含量（p〈0.05）。这表明金针菇多糖对小鼠的学习记忆有一定的促进作用,并能显著提高大脑海马组织中的蛋白质含量,提高SOD活力,降低MDA含量,表现出明显的抗氧化作用。 相似文献
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LI Mei-ai WANG Hua-dong LU Da-xiang WANG Yan-ping QI Ren-bin YAN Yu-xia FU Yong-mei LI Chu-jie 《园艺学报》2006,22(5):987-991
AIM: To investigate the protective effects of berberine against liver injury induced by lipopolysaccharide in mice and the mechanisms underlying its protective effect. METHODS: The male mice were divided randomly into control, berberine group, LPS group and berberine treatment group. Mice were administered intragastrically with distilled water (0.01 mL/g) or 5 g/L neutral sulfate berberine (0.01 mL/g) once a day for 5 days and injected intraperitoneally with normal saline or LPS （0.02 mL/g，28 mg/kg）at 1 h after gavage on day 5. Blood was collected for determining alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, the content of tumor necrosis factors-α (TNF-α) at 10 h and 2 h after LPS or normal saline injection, respectively. Furthermore, the liver tissue was processed, and histological changes and ultrastructure in liver were observed with light and electron microscopy, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in liver were also detected. RESULTS: Both ALT and AST activities in serum in LPS group were higher than those in control and berberine treatment group. LPS increased the serum TNF-ɑ content at 2 h after injection, which was reversed by berberine pretreatment. The histological examination showed that LPS caused severe hepatic cell edema, degeneration, apoptosis and even necrosis, and ultrastructure observation demonstrated that LPS induced mitochondrial swelling, condensation and margination of chromatin, irregular nuclear envelope in hepatocytes. The above pathological changes produced by LPS were attenuated by berberine pretreatment. Moreover, MDA contents in liver tissue were higher in LPS group than control and berberine treatment group, but there were no significant difference in SOD activity between berberine treatment and LPS group. CONCLUSION: Berberine has a protective effect on LPS-induced liver injury in mice, the mechanisms may be related to its decreasing the production of TNF-α, inhibiting lipid peroxidation and protecting mitochondria. 相似文献