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QIAN Ai-ping WENG Bo-qi WANG Yi-xiang YAN Sun-an JIANG Zhi-he Central Laboratory Fujian Academy of Agricultural Sciences Fuzhou China Agricultural Ecology Institute Fujian Academy of Agricultural Sciences Fuzhou China The Development Application Research Center of Edible Fungi Fujian Academy of Agricultural Sciences Fuzhou China 《食用菌学报》2006,(4)
2Results and Analysis2.1Essential amino acid composition of proteinfromspores ofA.blazeiJ1and J3Essential amino acid compositions ofprotein fromspores ofA.blazei,strains J1andJ3,are shown in Table1(see the Chineseversion).Ile,Leu and Phe+Tyr levels inprotein from J3spores were113.6%,16.7%and5.0%higher,respectively compared withthe corresponding protein fromJ1(P<0.05),whereas the content of other amino acids were6%-35%higher in protein fromJ1.The totalessential amino acid content of prot… 相似文献
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AIM: To observe the effects of tumor necrosis factor-α（TNF-α）-induced changes of expression and activity of 11-beta-hydroxysteroid dehydrogenase type1(11-β HSD-1) on the insulin sensitivity in 3T3-L1 adipocytes.METHODS: 3T3-L1 adipocytes were treated with TNF-α and TNF-α combined with aspirin, 2’-hydroxyflavanone or RU486, then mRNA expression and activity of 11-β HSD-1 and insulin-stimulated glucose uptake were examined.RESULTS: TNF-α increased expression and activity of 11-β HSD-1 in 3T3-L1 adipocytes, and decreased insulin-stimulated glucose uptake. Aspirin decreased expression and activity of 11-β HSD-1 induced by TNF-α, and alleviated the inhibiting effect of TNF-α on insulin-stimulated glucose uptake. 11-β HSD-1 specific inhibitor 2’-hydroxyflavanone and cortisol-receptor antagonist RU486 also alleviated the inhibiting effect of TNF-α on insulin-stimulated glucose uptake. CONCLUSION: TNF-α may decrease the insulin sensitivity in 3T3-L1 adipocytes through increasing expression and activity of 11-β HSD-1. 相似文献
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AIM: To investigate the effect of peroxisome proliferator-activated receptor γ(PPARγ) on the invasion ability of trophoblasts. METHODS: A segment of PPARγ siRNA was synthesized. Three groups were designed: experiment group, negative control group and blank control group. The PPARγ siRNA was transfected into JEG-3 cells. Real-time quantitive PCR was used to detect the mRNA levels of PPARγ and mucin-1(MUC1). The Transwell culture inserts were used to detect the invasion ability of JEG-3 cells 24 h after treated with PPARγ siRNA. RESULTS: After transfected with PPARγ siRNA, the mRNA expression levels of PPARγ and MUC1 were significantly depressed by (75.0±0.8)% and (65.0±1.3)% (P<0.05),respectively, and the invasion ability of JEG-3 cells was significantly strengthened (P<0.05). CONCLUSION: The depression of PPARγ gene down-regulates the expression of MUC1, and affects the invasion ability of trophoblasts, indicating that PPARγ may regulate the invasion of trophoblasts by MUC1 and involve in the development of placental defects such as preeclampsia. 相似文献
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AIM: To study the protective role of 14-3-3γ in burn or LPS-induced myocardial injury. METHODS: The rat model of burn or LPS-induced injury was established. The heart functions and 14-3-3γ protein expression were detected 3 h, 6 h, 12 h and 24 h after treatment. Primary neonatal rat cardiomyocytes were used in vitro. pFLAG-14-3-3γ plasmid was constructed and transfected into the cardiomyocytes 24 h before LPS-induced injury. The injury in the cardiomyocytes was evaluated by measuring the cell viability and the level of lactate dehydrogenase(LDH). Apoptosis of cardiomyocytes was detected by flow cytometry. Opening of mitochondrial permeability transition pore (mPTP) was also determined by Ca2+-induced swelling of isolated myocardial mitochondria. RESULTS: The expression of 14-3-3γ was elevated following the burn or LPS-induced myocardial injury in vivo. In vitro, transfection with pFLAG-14-3-3γ plasmid in to the cardiomyocytes significantly protected against LPS-induced injury. Compared with the cardiomyocytes without transfection with pFLAG-14-3-3γ plasmid, higher cell viability rate and lower LDH release, cell apoptosis and mPTP opening were observed in the cardiomyocytes transfected with pFLAG-14-3-3γ plasmid. CONCLUSION: The 14-3-3γ protein protects the heart against burn or LPS-induced injury by inhibiting the mPTP opening. 相似文献
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Efforts to improve the efficiency and efficacy of tree structure and crown architecture measurement are necessary to reduce error associated with indirect estimation of volume, which affects biophysical and ecosystem modelling, as well as resource assessment. In this short communication, we test the potential for a commercial SfM-MVS (Structure from Motion coupled with Multiple-View Stereophotogrammetry) software package, Photoscan-Professional, to accurately determine tree height, stem diameter, and eventually volume. SfM is a technique in computer vision, which calculates the 3D position of objects in a scene from a series of photographs. It uses a technique assuming that an object in a 3D scene is located on a vector between the image of the object in the camera and the object itself. The technique allows the construction of a 3D pointcloud, such as the one produced by laser technologies – terrestrial or airborne LiDAR. SfM requires no camera calibration or control points to construct an initial model. Moreover, it is a low-cost alternative to laser technologies. The second part of our method – MVS – is a visualization technique that reconstructs a 3D textured mesh of the scene from the SfM-derived pointcloud and RGB photographs. As a proof of concept, our methods were limited to two scenarios: (1) a single potted tree in a lab environment, where exact measurements could be made, and (2) two trees of different species and size in natural environments to test feasibility outside the laboratory. Precise measurements of tree height and stem diameter were compared with estimates obtained from the 3D model created using SfM-MVS. The results indicate that the SfM method is a promising – and inexpensive – alternative to terrestrial LiDAR and 3D scanners. Tree height estimates had error of 2.59%, while stem diameter estimates had error of 3.7%. The MVS algorithm used in this study was developed for plan surfaces such as topography or ‘compact’ objects and does not provide a representative 3D mesh for slender trees, although it works well for large stems. The authors link this disparity to the complex branching structure of trees.Future work requires (1) the development of effective automated volume reconstruction software specific to trees, and (2) the validation of the wide-scale applicability of the technology, which must include trees of various size, species, and growth forms. Moreover, future testing must include more complex environments, such as heterogeneous urban sites or closed-canopy forest sites where the proximity of other features may limit the utility of the new technology. 相似文献
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近几年来,鸡腿菇进入广州食用菌市场,已为不少市民所接受,同时促进了生产的发展。本所引进鸡腿菇新品种G 015进行了栽培试验,从中总结栽培技术。鸡腿菇G 015菌种引自湖北华中食用菌栽培研究所,菌丝生长温度10~35℃,最适22~28℃,子实体形成温度12~30℃,以15~24℃为最适,属中温型菌类。子实体丛生,色洁白。1季节安排9月初制作原种,经20 d左右的菌丝培育,9月中下旬则可接种培育种,再经20 d左右的培育后,栽培种则可脱袋安排出菇。2菌种制作原种和栽培种均以棉籽壳为主要原料。培养料配方:棉籽壳78%,麸皮20%,生石灰2%。棉籽壳等原料必须是新鲜、… 相似文献
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WANG Xu-dan LIANG Zhi-hui YANG Hui-ling TANG Bing ZHAO Rui-ying GUO Yu-biao ZHENG Cheng ZHENG Qin 《园艺学报》2008,24(5):972-977
AIM: To discuss the effect of Ad-14-3-3σ to microRNA (miRNA) in different radioresistant nasopharyngeal carcinoma (NPC) cells, CNE-1 and CNE-2, and study the relationship between the discrepancy of miRNA and radiosensitivity of NPC.METHODS: Ad-14-3-3σ was transfected to CNE-1 and CNE-2 cells, and then miRNAs were detected by Paraflo microfluidic microRNA chip. Hybridization images were collected using a laser scanner and the signals were normalized using a LOWESS filter. The effect of Ad-14-3-3σ to miRNAs and the relationship between the discrepancy of miRNA and radiosensitivity of NPC were studied according to Targetscan3.1 database (http://www.targetsan.org) after analyzing data.RESULTS: After treated by Ad-14-3-3σ, comparing to CNE-2 cells, there are 37 miRNAs changed remarkably, including 17 over-expression microRNAs and 20 under-expression microRNAs in CNE-1 cells. 6 miRNAs that one detective value was more than 1 000 and 3 folds than the other were hsa-miR-152,hsa-miR-205,hsa-miR-203,hsa-miR-7,hsa-miR-636 and hsa-miR-100.CONCLUSION: Ad-14-3-3σ can change the expression of miRNAs in different radioresistant nasopharyngeal carcinoma, and some miRNAs have relevance to carcinoma and radiosensitivity. 相似文献
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Effect of TNF-α on production and activation of caspase-3 in primary rat renal proximal tubule cells
LIU Shan-ying LI Yan PAN Qiu-hui WEI Jing FAN Xin-lan SU Fang LIN Yan-hua LIN Tian-xin 《园艺学报》2010,26(1):146-149
AIM: To investigate the production and activation of caspase-3 in primary rat renal proximal tubule cells in response to tumor necrosis factor-α(TNF-α) and the implication of nuclear factor-κB (NF-κB) in the process. METHODS: Isolated rat renal proximal tubule cells (PTCs) from male adult Sprague Dawley rats were treated with TNF-α according to the indicated time courses. A specific NF-κB inhibitor, Bay11-7082, was used alone or as a pretreatment for 1 h followed by exposure to TNF-α for 24 h. The protein levels of cleaved caspase-3, caspase-3, I-κBα, phosphorylated I-κBα, and GAPDH were detected by Western blotting using specific antibodies. RESULTS: The protein level of cleaved caspase-3 relative to caspase-3 was significantly increased in the presence of TNF-α for 6 h, 12 h, and 24 h. Protein levels of caspase-3 were significantly decreased by 12 h and returned to baseline by 24 h in the presence of TNF-α. Treatment with Bay11-7082 for 25 h alone or pretreatment with Bay11-7082 for 1 h followed by addition of TNF-α for 24 h caused a remarkable reduction in both cleaved caspase-3 and caspase-3 as compared to control and TNF-α treated groups. An increase in phosphorylated I-κBα was observed from 15 min to 60 min after treatment with TNF-α at a dose of 10 μg/L in PTCs. CONCLUSION: NF-κB is not only associated with the activation of caspase-3 but also the production of caspase-3 in primary rat renal proximal tubule cells in response to TNF-α. 相似文献
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WANG Hong CHEN Mingjie 《食用菌学报》2007,14(3):20-23
A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the coldinduced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea. 相似文献
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《食用菌学报》2006,13(3):80
November 3 ~6 ,2006Shanghai and Nantong,P.R.ChinaOrganized by MushroomBranch of the China Agricultural SocietyJapanese Society for MushroomScience and BiotechnologyThe Korean Society of MushroomScienceInstitute of Edible Fungi ,Shanghai Academy of Agricultural SciencesCo-organized by Jiangsu Alphay Bio-tech Co.,LtdShanghai Society for MicrobiologyShanghai Society for Horticultural ScienceTIMETABLE OF ACTIVITIESFRIDAY,3rdNovember ,20061400-1900 Registration (Foyer , … 相似文献
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