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1.
AIM: To investigate the diffusion effect of malignant tumor in cervix by diffusion weighted imaging (DWI) of magnetic resonance imaging (MRI).METHODS: Routine MRI sequences and axial diffusion weighted sequences were performed in the cases of cervical cancer and endometrial carcinoma. Normal cervixes and endometria were served as controls. The ADC values of cervical cancer and normal cervix, endometrial carcinoma and normal endometrium were measured and analyzed respectively.RESULTS: (1)The ADC values in 37 cases of cervical cancer and normal cervix of 16 volunteers were (0.92±0.20)×10-3 mm2/s and (1.26±0.24)×10-3 mm2/s respectively, with statistically significant difference between cervical cancers and normal cervixes (P<0.01). (2)The ADC values in 14 cases of endometrial carcinoma and normal endometrium of 14 volunteers were (0.87±0.17)×10-3 mm2/s and (1.34±0.26)×10-3 mm2/s respectively, with statistically significant difference between endometrial carcinoma and normal endometria (P<0.01).CONCLUSION: The diffusion effects of cervical cancer and endometrial carcinoma were different from those of normal cervical tissues. The DWI of 3.0T MRI may be used to quantitatively determine the limitation of diffusion effect in the malignant tumor in cervix by measuring the ADC value.  相似文献   

2.
AIM: To investigate the changes of endometrial receptivity under the effect of mouse embryo both in vitro and in vivo, and to figure out which part of the embryo induces the change. METHODS: Scanning electron microscope was applied to observe the pinpode formation on day 4 endometrium both in vitro and in vivo. The expression of integrin β3 and leukaemia-inhibitory factor(LIF) on day 2 pregnant mouse endometrium , day 4 endometrium after co-culture for 2 d with day 2 embryo , blastomere and zona pellucida as well as control group in vitro were detected by the methods of fluorescent quantitative PCR, immunohistochemistry and Western blotting. The same tests were conducted in the in vivo part of the experiment with the integral embryo or different parts of the embryo being transferred to pseudopregnant mouse uterus. On day 4 of the pregnancy, the endometrium was extracted to carry out the tests. RESULTS: After co-cultured for 2 d with whole embryo, the experssion of integrin β3 and LIF was higher than that in any other group in the in vitro part. The expression of integrin β3 and LIF on day 4 of normal pregnancy was higher than that in any other group in the in vivo experiments. CONCLUSION: Mouse embryo as a whole is able to induce better endometrial receptivity, while any separated part of embryo, such as blastomere or zona pellucida, couldn't.  相似文献   

3.
AIM: To determine whether microRNA-26a(miR-26a) is involved in development of liver cancer by analysis of proteomic expression profile of human hepatocarcinoma cell HepG2 transfected with miR-26a mimics.METHODS: HepG2 cells were cultured by a routine method and transfected with miR-26a mimics for 48 h for cell cycle analysis. The expressive proteome profiles of HepG2 cells with or without miR-26a mimics treatment were established by the methods of two-dimensional electrophoresis separation following lysis of the cells and extraction of the proteins. The proteomic expression profiles were analyzed by comparative proteomics technique to discover the important protein spots with differential expression. The identification of the proteins was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS). RESULTS: miR-26a brought down the proliferation of HepG2 cells. Total 11 protein spots with alteration of expressive amounts more than 2 times were successfully identified in the proteomic expression profile of HepG2 cells treated with miR-26a mimics, including annexin A1, peroxiredoxin 4, proliferating cell nuclear antigen, apolipoprotein A1, cytochrome C oxidase subunit 5A, cyclin E2, ribose-phosphate pyrophosphokinase 3, cyclin-dependent kinase 1 and phosphatidylethanolamine-binding protein 1. Among these, the expression of 3 protein spots was up-regulated and 8 of them was down-regulated.CONCLUSION: miR-26a contributes to the anti-cancer effect by expressive regulation of the proteins mentioned above, or directly or indirectly controls the proliferation, differentiation and death of hepatocarcinoma cells.  相似文献   

4.
AIM:To explore the expression of glucose transporter 4 (GLUT4) in the endometrium of rats with polycystic ovarian syndrom (PCOS) and evaluate the relationship between GLUT4 expression and insulin resistance (IR). METHODS:54 female SD rats of 85 days were randomized to control group (n=20), PCOS model group (n=17) and metformin treatment group (n=17). The rats in the latter two groups were induced by Poretsky’s method for PCOS model, followed by placebo or metformin, respectively. After 14 days of treatment, the rats were sacrificed and the expression of GLUT4 in endometrium was detected by ElivisionTM Plus two steps immunohistochemical staining. RESULTS:The expression of GLUT4 and insulin receptor(INS-R) proteins of endometrial glandulan epitheliu in PCOS rats were significantly lower (P<0.01,P<0.05) than those in control group, however, the expression of insulin(INS) protein in PCOS rats was higher than that in control group (P<0.01). The expression of GLUT4 in the treatment group increased (P<0.01), but was still lower than that in control group (P<0.01). However, compared with PCOS group, the expression of INS protein was decreased (P<0.05), but was still higher than that in control group (P<0.05). There was no GLUT4 expression in interstitial cells in endometrium, and the changes of the expressions of INS and INS-R proteins in those cells were similar with those in glandulan epitheliu. CONCLUSION:The decrease in GLUT4 expression of endometrium in PCOS rats is related with endometrial insulin resistance.  相似文献   

5.
AIM：To observe the effect of acupuncture on CD4+ CD25+ Foxp3(forkhead box P3)+ regulatory T-cells(Treg cells) in rats with embryo implantation failure. METHODS：One hundred and forty-four pregnant rats were randomly divided into control group(N), mifepristone treatment group(M), mifepristone+acupuncture treatment group(A) and mifepristone+progestin treatment group(W). The rats in groups M, A and W were treated with mifepristone-sesame oil solution on day 1, while the rats in group N were injected with the same amount of sesame oil. The Housanli(ST36) and Sanyinjiao(SP6) points were selected for acupuncture. From day 1 to the time of death, the rats in group A were fasten up and then the acupuncture was performed. Accordingly, the rats in group N and group M were only fixed, and the rats in group W were given progestin. Implanted embryos in each group were counted. The proportions of CD4+ CD25+ Foxp3+ Treg cells in peripheral blood and CD4+ Foxp3+ Treg cells in the endometrium were detected by flow cytometry. The mRNA and protein levels of Foxp3 were determined by real-time PCR and Western blotting, respectively. RESULTS：Compared with group N, the number of implanted embryos, the percentages of CD4+ CD25+ Foxp3+ Treg cells in peripheral blood and CD4+ Foxp3+ Treg cells in the endometrium, and the expression of Foxp3 protein and mRNA in the endometrium were significantly decreased in group M(P<005). Compared with group M, the above indexes in group A and group W were significantly increased. CONCLUSION: The effect of acupuncture in rats with embryo implantation failure may be closely correlated with the modulation of CD4+ CD25+ Foxp3+ Treg cells.  相似文献   

6.
AIM: To determine the expression of CCL17 and CCL22 in dendritic cells (DC) from human decidua and endometria. METHODS: The decidua were collected from normal pregnant women undergoing induced abortion and recurrent spontaneous abortion (RSA) women undergoing early abortion.The endometria were cllected from non-pregnant women undergoing abdominal hysterectomy.The mononuclear cells in the decidua and endometria were isolated. DC were induced by GM-CSF and IL-4, cultured in vitro and identified. The expression of CCL17 and CCL22 in DC at mRNA and protein levels was analyzed by real-time PCR and ELISA. RESULTS: The mRNA levels of CCL17 and CCL22 in decidual DC in normal pregnancy group were 3.04?0.40 and 1.83?0.24, respectively, significantly higher than those in endometrial DC in non-pregnancy group (0.85?0.24 and 0.31?0.08, respectively, P<0.01) and those in decidual DC in RSA group (1.65?0.14 and 0.96?0.09,respectively,P<0.01). Decidual DC continually and strongly secreted CCL17 and CCL22. The levels of CCL17 and CCL22 in normal pregnancy group were significantly higher than those in non-pregnancy group and RSA group at the same culture time point (P<0.01). CONCLUSION: The expression of CCL17 and CCL22 in decidual DC in pregnant woman increases. This may attract more CD4+CD25+ regulatory T cells to decidua and play an important role in the establishment of maternal-fetal immune tolerance.  相似文献   

7.
AIM: To investigate the expression of erythropoietin-producing hepatocellular receptor A2 (EphA2) and its ligand ephrin-A1 in endometrial endometrioid adenocarcinoma (EEA), and to analyze their relationship with angiogenesis of the tumor. METHODS: The CD34-stained microvessel density (MVD) and the expression of ephA2 and ephrin-A1 were detected by immunohistochemical assay in 56 cases of EEA, 20 cases of endometrial hyperplasia, 30 cases of normal proliferative endometrium and 30 cases of normal secretory endometrium. The correlations among the expression of EphA2 and ephrin-A1, MVD and clinicopathological features were analyzed. RESULTS: MVD and the expression of EphA2 and ephrin-A1 in EEA were significantly higher than those in the tissues from endometrial hyperplasia and normal endometrium (P<0.05). They were related to FIGO stage, histological differentiation, depth of myometrial invasion, lymphovascular invasion and progesterone receptor expression (P<0.05). A significant positive correlation between MVD and the expression of EphA2 and ephrin-A1 was observed by Spearman rank correlation test (r=0.476, P<0.05; r=0.501, P<0.05). CONCLUSION: Overexpression of EphA2 and its ligand ephrin-A1 in EEA may be involved in the angiogenesis and progesterone resistance.  相似文献   

8.
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9.
AIM:To investigate the influence of mifepristone on ultrastucture of human endometrium in the early secretory phase. METHODS: Endometrial tissue was obstained from 10 patients of reproductive age, who underwent a hysterectomy within 1 week postovulatory for gynecologic diseases not involving the endometrium. Patients were divided into mifepristone group (n=5) and control group (n=5) randomly. Each patient in the mifepristone group had taken 25 mg mifepristone per os 24 h before the operation was performed, while none of the control group had taken mifepristone. After removal of uterus, endometrial tissue was immediately acquired and prepared for electron microscopic examination. RESULTS:In comparison with the control group, the endometrial tissue in mifepristone group displayed the following distinctly morphological changes: (1) In the endometrial epithelium neither nucleolar channel system nor giant mitochondrium was seen, and subnuclear glycogen accumulation was seldom observed, but giant lysosomes were frequently found. (2) The intercellular spaces of the epithelium were narrow and straight, the indigitations of lateral plasma membranes were rarely visible. (3) Cytolysis and karyopyknosis of stroma cells and extravasal red cells were repeatedly observed.CONCLUSION:The above ment ioned morphological changes in endometrium in the early secretory phase caused by mifepristone are undoubtedly sufficient to prevent implantation.Consequently, mifepristone may have a contraception effect.  相似文献   

10.
AIM: To assess the effect of estrogen on the gene expression of caveolin-1 in rat vascular smooth muscle cells (VSMCs). METHODS: Wistar rats were ovariectomized and subjected to subcutaneous implantation of placebo pellets (OVX+V group) or estradiol pellets (OVX+E group). 2 weeks after implantation, the expression of caveolin-1 gene in endothelium-denuded aortic tissue was examined by RT-PCR. Furthermore, Northern blotting was used to analyze the mRNA expression of caveolin-1 in cultured rat VSMCs. RESULTS: RT-PCR showed that expression of caveolin-1 gene was significantly higher in OVX+E group than that in OVX+V group. Northern blot analysis showed that the mRNA expression of caveolin-1 was higher in VSMCs pretreated with 17β-estradiol (17β-E2) than that in VSMCs without 17β-E2 pretreatment. CONCLUSION: Estrogen up-regulates the gene expression of caveolin-1 in the vascular wall, partially indicating the cardiovascular effect of estrogen.  相似文献   

11.
AIM: To investigate the differential expression of annexin A2 (ANXA2) in gastric carcinoma and to analyze the relationship between ANXA2 expression and clinicopathological parameters of gastric carcinoma. METHODS: Pure gastric adenocarcinoma cells (GAC) and normal gastric epithelial cells (NGEC) in 15 patients with gastric cancer were acquired by laser capture microdissection (LCM). All peptide specimens after trypsin digestion were labeled with 18O/16O. Quantitatively identification of differential expression of the proteins betweem GAC and NGEC was performed by Nano-RPLC-MS/MS. The expression of ANXA2 in the 2 kinds of tissues was detected by Western blotting. Tissue microarray containing 75 pairs of gastric carcinoma and para-carcinoma tissues was used and the expression of ANXA2 in these specimens was detected by the method of immunohistochemistry (IHC). The relationship between ANXA2 expression and clinicopathological parameters of the pateints with gastric carcinoma was analyzed. RESULTS: A total of 78 differential proteins were identified and ANXA2 was up-expressed in GAC (2.32∶ 1), which was confirmed by Western blotting (P<0.01). The results of IHC showed that the correlations between the expression level of ANXA2 protein and invasive depth (T stage), lymph node metastasis (N stage), histological differentiation, TNM stage and the size of tumor were observed (P<0.01), but the correlations between the ANXA2 expression and sex, age and distant metastasis (M stage) were not found (P>0.05). CONCLUSION: The up-expressed ANXA2 may play an important role in the biological behavior of gastric cancer.  相似文献   

12.
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13.
AIM: The purpose of the present study was to explore the relationship between interleukin-6 mRNA expression and endometriosis. METHODS: Using the rat model, IL-6 mRNA expression in the endometrium was examined by RT-PCR. RESULTS: The expression of IL-6 mRNA in control rats did not change at 2, 4, 6 and 8 weeks after sham operation (P>0.05), but in model rats it gradually increased at 2, 4, 6 and 8 weeks after endometriosis (P<0.01). The expression of IL-6 mRNA in uterine endometrium with endometriosis was lower than in endometriotic tissue, but higher than in endometrium from healthy controls. CONCLUSION: The IL-6 mRNA expression may contribute to the development of endometriosis . The increase in IL-6 mRNA expression may promote the implantation and growth of endometriotic tissue.  相似文献   

14.
AIM: To investigate ischemia reperfusion (I/R)-induced proteomic changes in rat skeletal muscle. METHODS: Healthy male Wistar rats were randomly divided into two groups as follows (n=6): sham group and I/R group. I/R of right hind limb was induced by 4 h ischemia followed by 24 h reperfusion. The 2-DE was applied to separate the proteins extracted from skeletal muscle tissue at the end of experiment, followed by Coomassie Brillant blue R-250 staining. Computer image analysis was used to determine the differential expression of proteins between the two groups, and 7 protein spots expressed differentially were picked out and subjected to in-gel digest and MALDI-TOP for identification. RESULTS: 354±13 proteins were detected and the match rate was (78.7±1.4)%. 10 proteins displayed significant changes after I/R, of which, 6 proteins increased and 3 proteins decreased in expression. Moreover, 2 spots in I/R group were observed, only 1 spots of which in control. 5 proteins were identified after mass spectrometry. Mitochondrial aldehyde dehydrogenase (ALDH) precursor, heat shock 27 kD protein (HSP27), an unnamed protein product (increased in I/R group), α-actin (decreased in I/R group), and nuclear transport factor 2 (NTF-2) W7a mutant were found in I/R group. CONCLUSION: I/R injury induced differential proteomic changes in rat skeletal muscle. ALDH, α-actin and HSP27 expression, and NTF-2 mutation are involved in I/R injury.  相似文献   

15.
 对结球甘蓝（Brassica oleracea L. var. capitata L.）花粉总蛋白双向电泳及差异蛋白质谱分析, 发现膜联蛋白在花粉萌发后较萌发前表达下调。通过同源扩增和RACE 等方法首次在结球甘蓝花粉中扩增得到BoAnnexin2 基因的cDNA 序列,该基因cDNA 全长1 157 bp,开放阅读框为951 bp,编码316 个氨基酸残基,预测分子量为36.02 kD,等电点6.33。BoAnnexin2 编码蛋白C 端有4 个膜联蛋白重复序列,共2 个Ⅱ型钙结合区域,在第4 个钙结合区位点包含GXXXGXS（T）/DXXG 基序。实时荧光定量试验表明,BoAnnexin2 在甘蓝成熟未萌发花粉中的表达量是萌发45 min 后表达量的8 倍,表明BoAnnexin2 在甘蓝花粉萌发起始阶段起到重要调控作用。  相似文献   

16.
AIM: To investigate the effect of human chorionic gonadotropin(hCG) on cyclooxygenase-2(COX-2) expression in endometrial carcinoma so as to study the function of ectopic hCG.METHODS: The ectopic β-hCG in JEC endometrial carcinoma cell lines was quantified by radioimmunoassay. By MTT assay, the effect of hCG on the cell viability of JEC cell lines and the inhibition of selective COX-2 inhibitor NS398 on JEC cell lines were examined. The effect of hCG on COX-2 expression was detected by Western blotting. RESULTS: The ectopic β-hCG release from cultured JEC cell lines were observed. HCG promoted the cell viability, upregulated the expression of COX-2 protein and increased the inhibition of selective COX-2 inhibitor NS398 in JEC cell lines. CONCLUSION: The ectopic hCG in JEC endometrial carcinoma cell lines increases cell proliferation, which may be mediated by upregulating the expression of COX-2 protein.  相似文献   

17.
AIM: To study the reverse effect of FOXC2 silencing on epithelial-mesenchymal transition (EMT) induced by transforming growth factor β1(TGF-β1) in MCF-7 cells. METHODS: Cultured MCF-7 cells were treated with TGF-β1 at concentration of 5 μg/L for 6 d. The cell morphological changes were observed under phase-contrast microscope. The changes of EMT-related marker proteins were assessed by immunofluorescence staining assay. TGF-β1-induced MCF-7 cells were transfected with FOXC2-siRNA mediated by recombinant lentivirus. In addition, the expression levels of FOXC2 and EMT-related marker proteins E-cadherin, claudin-1 and fibronectin-1 were also measured by RT-PCR and Western blotting. The invasion of MCF-7 cells was detected by Transwell assay. RESULTS: TGF-β1 induced the morphological alteration in MCF-7 cells from epithelial phenotype to mesenchymal phenotype,up-regulated the expression of mesenchymal marker fibronectin-1, and down-regulated the expression of epithelial markers E-cadherin and claudin-1. FOXC2 silencing reversed and restored the mesenchymal MCF-7 cells to epithelial phenotype and reduced the tumor invasion. CONCLUSION: EMT model induced by TGF-β1 in breast cancer MCF-7 cells is successfully established, which increases the invasion of MCF-7 cells. The effect of TGF-β1 is reversed by FOXC2-siRNA and the invasion of the cells is reduced.  相似文献   

18.
AIM: To investigate the depressant effect of FK228 combined with rapamycin on the human breast cancer cell line MCF-7 and MDA-MB-435.METHODS: FK228, a new histone deacetylase inhibitor, and rapamycin, the specific inhibitor of the mammalian target of rapamycin (mTOR) protein, were used in the study. MCF-7 cells and MDA-MB-435 cells were exposed to different concentrations of FK228 and rapamycin. The inhibitory rate of cell growth was determined by SRB assay. Combination index (CI) was used to evaluate the interaction between FK228 and rapamycin. The expression of the apoptotic proteins, cycle proteins and nucleic acid proteins were detected by Western blotting. The cell cycle was analyzed by flow cytometry.RESULTS: Both FK228 and rapamycin showed growth inhibitory effects on the breast cancer cell lines in a time-and dose-dependent manner. CI of the 2 drugs was less than 1 when the inhibitory rate of the cell growth was 50% effective dose (ED50)~ED70, indicating a synergistic effect. The combination therapy of FK228 with rapamycin increased the apoptotic proteins, and induced the down-regulation of phosphorylated Akt and over-expression of caspase-3 compared with a single use of the drugs. The combination therapy of FK228 with rapamycin reduced the cycle proteins, and the cell cycle was arrested in G2/M. The levels of phosphorylated H2AX and acetylated H3 were ob-viously increased after combination therapy.CONCLUSION: The combination therapy of FK228 with rapamycin inhibits the cell proliferation and increases apoptosis with a synergistic effect, which may become a new trend for treating endometrial cancer.  相似文献   

19.
AIM: To analyze the proteomic components of the sera from knee osteoarthritis patients and normal people, and to search proteins that might serve as serum biomarkers for osteoarthritis diagnosis, treatment or pathogenesis. METHODS: Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) was applied to analyze the sera obtained from the patients with knee osteoarthritis (n=4) and normal controls (n=4). The differentially expressed proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Western blotting analysis was applied to confirm the results. RESULTS: Comparative proteomic data of serum from the patients with osteoarthritis was successfully obtained. Eight differentially expressed protein spots were observed. Five up-regulated and 3 down-regulated proteins were identified. Western blot analysis confirmed that α2-macroglobulin was increased. CONCLUSION: There are significant differences between serum proteins obtained from the patients with knee osteoarthritis and normal controls. α2-Macroglobulin might be utilized as potential biomarkers for the diagnosis and treatment of osteoarthritis.  相似文献   

20.
AIM: The comparative analysis of total proteins expressed differentially between adenocarcinoma cell line A549 (a human lung adenocarcinoma) and normal cell line HBE (a human lung bronchial epithelium) was conducted to search the proteins involved in tumorigenesis and potential biomarkers of diagnosis or prognosis. METHODS: The proteins of dramatic differential expression were screened in adenocarcinoma cell Line A549 and normal cell line HBE by using immobilized pH gradient-two dimensional polyacrylamide gel electrophoresis combined with MALDI-TOF/TOF tandem mass spectrometry. Furthermore, the differential expressed proteins were confirmed by the method of Western blotting. RESULTS: Compared with the two cell lines, 21 differential expressed proteins were found and their functions involves in cell metabolism, protein modification, cell motility, protein trafficking and signal transduction. The up-regulation of heat shock protein beta-1 (HSPB1) in A549 cells was identified and confirmed. CONCLUSION: These results suggest that dramatic differential proteomic expression exists between the two cell lines. The high level of HSPB1 might play an important role in the process of tumorigenesis.  相似文献   

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