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1.
We have recently suggested that feeding the C18 polyunsaturated fatty acid, alpha-linolenic acid (ALA), instead of linoleic acid (LA) reduced body fat in mice. However, the difference in body fat did not reach statistical significance, which prompted us to carry out this study using more animals and diets with higher contents of ALA and LA so that the contrast would be greater. The diets contained either 12% (w/w) LA and 3% ALA or 12% ALA and 4% LA. A low-fat diet was used as control. The diets were fed for 35 days. The proportion of body fat was not influenced by the type of dietary fatty acid. Plasma total cholesterol and phospholipids were significantly lower in ALA-fed mice than in mice fed LA. Activities of enzymes in the fatty acid oxidation pathway were significantly raised by these two diets when compared with the control diet. alpha-Linolenic acid vs. LA did not affect fatty acid oxidation enzymes. In mice fed the diet with LA activities of enzymes of de novo fatty acid synthesis were significantly decreased when compared with mice fed the control diet. alpha-Linolenic acid vs. LA feeding did not influence lipogenic enzymes. It is concluded that feeding mice for 35 days with diets either rich in LA or ALA did not significantly influence body composition.  相似文献   

2.
Conjugated linoleic acid changes swine performance and carcass composition   总被引：22，自引：0，他引：22  
Conjugated linoleic acid (CLA) is a collective term for positional and geometric isomers of linoleic acid. Dietary CLA has been shown to improve feed efficiency, decrease body fat, and increase lean tissue in laboratory animals. We hypothesized that CLA would improve performance and carcass composition and would be deposited in pork tissues. Diets of 40 crossbred pigs were supplemented with CLA to determine its effects on performance and carcass composition. Eight replications of five littermate barrows with an initial average weight of 26.3 kg were allotted at random to individual pens. Within replication dietary treatments containing 0, 0.12, 0.25, 0.5, or 1.0% CLA were assigned at random. Pigs were weighed and feed disappearance was determined at 14-d intervals. Average daily gain increased linearly as the level of CLA increased in the diet (P < 0.05). Average daily feed intake was not affected by the concentration of CLA in the diet. Therefore, a linear increase in gain:feed ratio (P < 0.05) was observed. Carcasses from animals fed control diets had greater 10th rib backfat than carcasses from animals fed CLA (P < 0.05). Ultrasound measurement and carcass measurements showed less fat depth over the loin eye at the 10th rib of pigs fed doses of CLA (P < 0.05) than that observed for control pigs. Belly hardness (firmness) increased linearly as the concentration of CLA in the diet increased when bellies were measured for firmness either lean side up (P < 0.001) or lean side down (P < 0.05). Loin dissection data demonstrated that CLA produced a quadratic treatment effect both for less intermuscular fat (P < 0.001) and less subcutaneous fat (P < 0.05) and a linear increase for bone (P < 0.05), although finished loin weight only tended to increase (P = 0.08). The CLA concentration increased in a linear relationship in both subcutaneous fat (P < 0.001) and lean tissue (P < 0.001). Dietary CLA was incorporated into pig tissues and had positive effects on performance and body composition.  相似文献   

3.
试验采用2×2因子设计(日粮类型0%或1%CLA,肉鸡性别)研究日粮CLA对不同性别肉仔鸡生长性能、屠体性状和脂肪代谢相关酶的影响。结果表明:日粮中添加1%CLA可以显著增加各性别肉仔鸡各阶段的体增重,并改善了4～6、0～6周的饲料转化效率(P<0.05),可以显著提高各性别肉仔鸡的胸肌率、同时显著降低各性别肉仔鸡的腹脂率(P<0.05),显著降低了各性别肉仔鸡腹脂脂蛋白脂酶(LPL)活性及其mRNA相对表达量(P<0.05),显著降低了各性别肉仔鸡腹脂及肝脏硬脂酰辅酶A去饱和酶(SCD)mRNA相对表达量(P<0.05)。另外除体增重部分指标外,性别和日粮CLA对肉仔鸡屠体性状和脂肪代谢相关酶的影响不存在互作关系。  相似文献   

4.
An experiment was undertaken to determine if dietary conjugated linoleic acid (CLA) influences the early stage of the inflammatory response caused by a single injection of Salmonella enteritidisi lipopolysaccharide (LPS) in male broiler chicks. Chicks (7 days old) were fed either 0 or 10 g CLA/kg in their diet for 21 days. At 28 days old, birds were injected intraperitoneally with 1.5 mg LPS/kg body weight after 12 h fasting. Changes in feed intake, rectal temperature and plasma α‐1 acid glycoprotein and ceruloplasmin concentrations were measured during 24 h after LPS injection. Chicks fed the CLA diet were fed more feed for 24 h, had a lower rectal temperature at 9 and 24 h and a lower plasma α‐1 acid glycoprotein concentration at 24 h after LPS injection than chicks fed the basal diet. Plasma ceruloplasmin concentration tended to be lower in chicks fed the CLA diet than in chicks fed the basal diet. The results suggest that dietary CLA alleviate undesirable early inflammatory response due to LPS injection in male broiler chicks.  相似文献   

5.
共轭亚油酸对肉仔鸡体组成和肉品品质的影响   总被引：1，自引：3，他引：1  
本试验旨在探讨日粮添加共轭亚油酸(CLA)对肉仔鸡体组成和肉品品质的影响。240只1日龄肉仔鸡随机分配到4个日粮处理组(0%、0.25%、0.5%和1%CLA),每个处理6个重复,每个重复10只鸡。试验结果表明:42d时,0.5%和1%CLA组肉仔鸡胸肌率显著高于对照组(P<0.05),1%CLA组肉仔鸡42d腿肌率显著高于对照组,肉仔鸡的腹脂率在各处理之间没有显著差异(P>0.05),1%CLA组肉仔鸡胸肌粗蛋白质粗脂肪比显著高于对照组(P<0.05)。0.5%、1%CLA组肉仔鸡胸肌滴水损失和丙二醛(MDA)含量显著降低(P<0.05)。试验结果表明,CLA可改善肉仔鸡屠体性状,增加鸡肉系水力和延长货架存放时间。  相似文献   

6.
We investigated the effects of dietary conjugated linoleic acid (CLA) on fatty acid composition and lipid oxidation in breast meat of broiler chickens. Broiler chickens (28-day-old females) were fed diets containing experimental oils at 20 g/kg diet for 28 days. The experimental oils consisted of either a 2:0, 1:1, or a 0:2 (wt : wt) ratio of safflower oil (high linoleic acid content) to a commercial CLA mixture. In this study, dietary CLA supplementation significantly increased the composition and content of CLA in chicken meat. The predominant CLA in meat from birds with supplemented diets was the cis -9, trans -11 isomer. The proportion of saturated fatty acid in meat significantly increased with increasing CLA supplementation, with a corresponding decrease in monounsaturated fatty acid. Dietary CLA also reduced thiobarbituric acid reactive substances (TBARS) values in raw meat during storage at 4°C for 5 days. These results provide evidence that CLA feeding is a practical strategy not only for adding nutritional benefits to chicken meat but also for improving meat quality including oxidative stability.  相似文献   

7.
This study was designed to determine the effects of calcium salt of palm oil fatty acids (CS), hydroxyethylsoyamide (HESA), butylsoyamide (BSA) and soybean oil (SO) on degradation of crude protein and fibre in vitro, and on the blood plasma lipid parameters in vivo. Five mature wethers (body weight 75 kg) were fed five diets in a 5 x 5 Latin square experiment. The control diet consisted of 50% meadow hay and 50% concentrate with no added fat. The control diet was supplemented with CS, HESA, BSA, or SO. Fat was added at 3.5% of dietary dry matter (DM). The final ether extract content of the ration was near 6%. Each period lasted 20 days. Fat supplements, except HESA, consistently decreased the in vitro DM disappearance of soybean meal as compared to control. In contrast to the effect of other treatments, crude protein degradation was greatest in the test tubes with inocula obtained from sheep fed diet with HESA. Fat supplements equally inhibited the DM and fibre breakdown of alfalfa pellet. CS and HESA seemed to be less detrimental to in vitro fermentation of neutral detergent fibre (NDF) than BSA and SO. All fat supplements increased blood plasma triglyceride, cholesterol and total lipid content. Plasma concentration of cholesterol and total lipid was highest with SO. The inclusion of CS in the diet increased 16:0, while all fat supplements increased plasma 18:0 and decreased 16:1 and 18:1 fatty acid content. Plasma 18:2n-6 was not changed by feeding CS and SO. However, compared to the control diet, 18:2n-6 increased with 12 and 41% in plasma fatty acids when sheep were fed HESA and BSA, respectively. The results showed that plasma concentration of linoleic acid was enhanced more when the amide was synthesised from butylamine than when from ethanolamine.  相似文献   

8.
Two separate studies tested the hypoth-esis that plasma low-density lipoprotein cholesterol (LDL-C) can be decreased by conjugated linoleic acid (CLA) by depressing hepatic acyl-coenzyme A: cholesterol acyltransferase (ACAT) activity. In the first experiment, 3 groups of 6 early-weaned piglets were fed low-fat diets containing either 1.5% CLA, 1.5% corn oil or 1.5% beef tallow;fat provided 8% of the energy intake. In the second experiment, 4 groups of 6 early-weaned piglets were fed high-fat di-ets containing either 15% beef tallow, 12% beef tal-low plus 3% CLA, 15% corn oil, or 12% corn oil plus 3% CLA; fat provided 29% of energy intake. Cholesterol was balanced across diets in both experi-ments. In pigs fed the low-fat diets, all dietary fats in-creased LDL-C and triacylglycerols and decreased high-density lipoprotein cholesterol (HDL-C) and very low-density lipoprotein cholesterol (VLDL-C). LDL-C was the same in pigs fed low-fat tallow or low-fat CLA diets. However, ACAT activity was near-ly 80% higher in pigs fed the low-fat tallow diet than in pigs fed the low-fat CLA diets. All high-fat diets increased LDL-C, HDL-C and triacylglycerols equally with no effect on VLDL-C. There were no unique fat-ty acid effects of the high-fat diets on ACAT activity.We conclude that supplemental fats had differential effects on hepatic ACAT activity and LDL-C, but on-ly in pigs fed low-fat diets.  相似文献   

9.
Two separate studies tested the hypothesis that plasma low-density lipoprotein cholesterol (LDL-C) can be decreased by conjugated linoleic acid (CLA) by depressing hepatic acyl-coenzyme A:cholesterol acyltransferase (ACAT) activity.  In the first experiment, 3 groups of 6 early-weaned piglets were fed low-fat diets containing either 1.5% CLA, 1.5% corn oil or 1.5% beef tallow; fat provided 8% of the energy intake.  In the second experiment, 4 groups of 6 early-weaned piglets were fed high-fat diets containing either 15% beef tallow, 12% beef tallow plus 3% CLA, 15% corn oil, or 12% corn oil plus 3% CLA; fat provided 29% of energy intake.  Cholesterol was balanced across diets in both experiments.  In pigs fed the low-fat diets, all dietary fats increased LDL-C and triacylglycerols and decreased high-density lipoprotein cholesterol (HDL-C) and very low-density lipoprotein cholesterol (VLDL-C). LDL-C was the same in pigs fed low-fat tallow or low-fat CLA diets.  However, ACAT activity was nearly 80% higher in pigs fed the low-fat tallow diet than in pigs fed the low-fat CLA diets.  All high-fat diets increased LDL-C, HDL-C and triacylglycerols equally with no effect on VLDL-C.  There were no unique fatty acid effects of the high-fat diets on ACAT activity.  We conclude that supplemental fats had differential effects on hepatic ACAT activity and LDL-C, but only in pigs fed low-fat diets.  相似文献   

10.
This study described the relation between the type and amount of dietary fat on the deposition of abdominal fat by broiler chickens. It was hypothesized that at higher fat intakes, the well-known lowering effect of polyunsaturated fatty acids on the deposition of abdominal fat would be diminished. Experimental diets were formulated to contain three levels of added fat (3%, 6% and 9%). Each level had different proportions of the saturated fatty acids (SFA) and unsaturated fatty acids (UFA) by installing the ratios of 1:1, 1:2, 1:3, 1:4 and 1:5 with the use of tallow and soybean oil. Arbor Acres chicks, aged 7 days, were fed one of the 15 experimental diets until they were aged 42 days. Feed and water were provided ad libitum. There was no systematic effect of the dietary fat type and the amount on the weight gain and the feed intake. The lowest SFA:UFA ratio of 1:5 produced the lowest feed conversion rates, irrespective of the amount of the fat in the diet. The abdominal fat deposition was similar in the birds fed on diets containing either 3% or 6% added fat, but deposition was lower than in those fed 9% fat. A decrease in the SFA:UFA ratio of the diet was associated with a dose-dependent decrease in abdominal fat, irrespective of the amount of fat in the diet. This observation leads to the rejection of the hypothesis stated above. A decrease in the dietary SFA:UFA from 1:1 to 1:4 caused a decrease in the number of the fat cells per surface unit of breast meat. It is concluded that an increased intake of soybean oil at the expense of tallow reduced the abdominal fat deposition and the number of fat cells in the breast meat of broiler chickens.  相似文献   

11.
The hypothesis tested was that the intake of extra fat at the expense of an isoenergetic amount of nonstructural carbohydrates reduces fibre utilisation in horses. In a crossover trial with feeding periods of 42 days each, 6 mature trotting horses (age 4-12 years, bodyweight 340-476 kg) were given either a control or test diet. The test concentrate was formulated to contain 37% of net energy in the form of soybean oil. The control concentrate contained an isoenergetic amount of corn starch plus glucose. The concentrates were fed in combination with the same amount of hay so that the control and test diet contained 25.13 and 86.66 g crude fat/kg dry matter, respectively. Apart from the amounts of fat and nonstructural carbohydrates, the 2 diets were identical. The test diet reduced the apparent total tract digestibilities of crude fibre, neutral and acid detergent fibre by 8.0 (P = 0.007), 6.2 (P = 0.022) and 8.3 (P = 0.0005) percentage units, respectively. It is suggested that a high fat intake by horses may increase the amount of fat entering the large intestine to levels that depress fermentation by cellulolytic bacteria. The observed interaction between fat content of the diet and fibre utilisation may have consequences for practical horse feeding in that calculating the energy content of test diets on the basis of feedstuff tables leads to overestimating the amount of energy provided by the high-fibre ingredients of the diets.  相似文献   

12.
本文旨在探讨饲粮蛋白质水平对育成期母貂生长性能、营养物质消化代谢及血清中与氮代谢相关的生化指标的影响。选择健康（５０±３）日龄断奶的雌性水貂 ６０只,平均体重为（０．５７７±０．０７３）ｋｇ,随机分成 ６组,每组 １０个重复,每个重复 １只,单笼饲养。６组水貂分别饲喂蛋白质水平为 ２８％、３０％、３２％、３４％、３６％、３８％的试验饲粮。在水貂 ６５、８０、９５、１１０日龄时各进行 １次消化代谢试验,分析各日龄水貂的蛋白质消化率、脂肪消化率、氮沉积、蛋白质生物学价值、饲料转化率及体增重等指标。结果表明：５０～６５日龄,３８％蛋白质组体增重极显著高于２８％和 ３２％蛋白质组（Ｐ＜０．０１）,３８％蛋白质组饲料转化率极显著优于 ２８％和 ３２％蛋白质组（Ｐ＜０．０１）;５０～１１０日龄,３２％和 ３４％蛋白质组体增重显著高于 ２８％蛋白质组（Ｐ＜０．０５）。９５日龄时,３２％、３４％和 ３８％蛋白质组的蛋白质消化率极显著高于 ２８％和 ３０％蛋白质组（Ｐ＜０．０１）。１１０日龄时,３４％蛋白质组蛋白质生物学价值和净蛋白质利用率极显著低于其他试验组（Ｐ＜０．０１）。１１０日龄时,３８％蛋白质组尿素氮含量极显著高于其他各组（Ｐ＜０．０１）;３４％和３６％蛋白质组总氨基酸含量显著高于 ２８％蛋白质组（Ｐ＜０．０５）。综合试验中的测定指标,建议５０～６５日龄,水貂饲粮蛋白质水平为 ３６％ ～３８％;６６～８０日龄,水貂饲粮蛋白质水平为 ３４％ ～３６％;８１～１１０日龄,水貂饲粮蛋白质水平为 ３２％ ～３４％。  相似文献   

13.
One hundred sixty pigs were used in a 2 x 2 factorial design to compare the performance response to daily injection of porcine somatotropin (PST); (0 or 2 mg/d) in animals fed a 14% CP corn-soy diet (control, C) to those fed a diet with 10% added fat (F) and calorie:protein and lysine:protein ratios similar to that of the C diet. Treatments, assigned randomly to 20 pens (n = 5/treatment) of eight pigs each, were initiated at 90 kg body weight and continued for 28 d. Responses to PST and dietary fat were typical. These include improved gain and feed efficiency and decreased feed intake. The effects of dietary fat on intake and efficiency were accounted for largely by the difference in energy density of these diets. Across diets, PST treatment resulted in a 13% improvement in ADG (P less than .001), a 13% decrease in feed intake (P less than .0001) and a 22% improvement in efficiency (P less than .0001). Of particular interest were the additive (PST x diet interaction, P less than .2) effects of PST and dietary fat on gain in these animals. Pigs treated with PST that were fed the F diet had greater rates of gain than did PST-treated pigs fed the C diet (P less than .05). Treatment with PST increased ADG by 9% in pigs consuming the C diet vs 16% in pigs fed the F diet. Similarly, dietary fat resulted in 4 and 11% increases in ADG in pigs treated with 0 or 2 mg of PST/d.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

14.
The objective of this experiment was to determine the effects of dietary lipid source with or without the addition of CLA on bacon composition and quality. Forty-eight barrows at a beginning BW of 55 kg +/- 2.2 were fed 1 of 6 diets for 56 d. These diets consisted of: 1) normal corn (NC), 2) NC + 1.25% CLA-60 oil (NC + CLA), 3) high-oil corn (HOC), 4) HOC + 1.25% CLA-60 oil (HOC-CLA), 5) NC + choice white grease (CWG; NC + CWG), and 6) NC + CWG + 1.25% CLA-60 oil (NC + CWG + CLA). The CLA-60 contains 60% CLA isomers in the oil, and therefore, 1.25% oil was needed to achieve 0.75% CLA in the diet. Soy oil replaced CLA in control diets. Choice white grease and high-oil corn were selected as fat sources for this study because of their utility in energy density for growing-finishing pigs, especially in hot weather. Pigs were slaughtered at an average BW of 113 kg +/- 4.1, and carcasses were fabricated at 24 h postmortem. Statistical analysis was performed using the mixed model procedure of SAS, and the main effects tested were dietary lipid source, CLA, and 2-way interaction. The addition of CLA to each basal diet improved (P < 0.05) belly firmness measured either lean side down or fat side down from the belly bar firmness test [4.39 cm vs. 7.01 cm (lean down) and 5.75 cm vs. 10.54 cm (fat down)] for 0 and 0.75% dietary CLA, respectively. The compression test used on bacon slabs showed that bacon from CLA-supplemented pigs was approximately 20% firmer than that from controls. Pigs fed the HOC diets had softer bellies compared (P < 0.05) with pigs fed the NC diet as measured by the belly bar test [6.94 cm vs. 9.26 cm (fat down)], respectively. Conjugated linoleic acid did not, however, improve bacon sliceability. No differences were observed for moisture, protein, or lipid percentages between any treatments. Overall, there was a CLA effect (P < 0.04) for lipid oxidation, in which the addition of CLA decreased bacon oxidation (0.1498 CLA vs. 0.1638 no CLA). Dietary CLA increased the percentage of SFA in tissues from pigs supplemented with CLA. Dietary inclusion of CLA increased the concentration of all measured isomers of CLA in bacon. Sensory scores of bacon showed no differences for any of the sensory attributes measured between any of the treatments. Our results indicate that inclusion of dietary CLA will improve belly firmness, extend the shelf life stability of bacon, and increase the degree of fat saturation.  相似文献   

15.
Energy balance of conjugated linoleic acid-treated pigs   总被引：2，自引：0，他引：2  
The term conjugated linoleic acid (CLA) refers to a group of linoleic acid derivatives whose conjugated double-binding system begins at the carbon atom 8 to 11. This means that including the cis-trans geometry, 16 isomers are possible in theory. CLA occurs primarily in lipids of ruminants because polyunsaturated fatty acids can be converted to CLA in the rumen of these animals through the action of bacterial enzymes (Kepler et al. 1966). The most widely occurring isomer is C18 : 2 c 9, t 11, which is present in milk and dairy products in concentrations of 0.4–1.7% (Fritsche and Steinhart 1998). But CLA was also generated in the colon of rats fed a diet containing free linoleic acid (Chin et al. 1994b). In animal experiments CLA inhibits carcinogenesis and reduces the development of arteriosclerosis (Ip et al. 1994; Belury 1995; Jahreis and Bochmann 1998), and was found to have a specific influence on body composition. In experiments with mice, pigs and chicks a negative effect of CLA on the accumulation of body fat was observed (Pariza et al. 1996; Dugan et al. 1997; Park et al. 1997; West et al. 1998). The effective dose in those experimental diets was 0.5 to 1.5% CLA. In contrast, CLA may also act as a growth factor and increased daily weight gains of rats (Chin et al. 1994a). However, in these trials on changes in body composition the feed intake was not controlled and the observed effects may be due at least partially to differences in feed intake between the control group and the CLA animals. The objective of the present study was to investigate the influence of CLA on thermogenesis and energy balance in animals with an isoenergetic feed intake by using the methodology of the calorimetric balance technique. The animals used in the model were adult sows with a nutritional status approaching equilibrium.  相似文献   

16.
研究0.33%鱼油与2mg/kgDM的有机硒对饲喂亚麻籽日粮的绵羊体组织脂肪酸组成及抗氧化功能的影响。初步探讨在绵羊日粮中添加亚麻籽提高体组织CLA含量的同时,再添加鱼油和硒是否能进一步提高CLA含量。选用体重相近,健康的杂交一代公羔羊16只,随机分为4组。4组日粮为对照组LD组(基础日粮+10%亚麻籽),LF组(基础日粮+0.33%鱼油+9.17%亚麻籽,其中0.33%鱼油+9.17%亚麻籽所含脂肪与10%亚麻籽脂肪含量相等),LDSe组(基础日粮+10%亚麻籽+2mg Se/kgDM);LFSe组(基础日粮+0.33%鱼油+9.17%亚麻籽+2mgSe/kg DM),硒以有机硒的形式添加。结果表明:硒的添加对绵羊血浆抗氧化功能无显著影响(P<0.05),可能是受基础日粮硒浓度的影响;亚麻籽日粮中添加0.33%鱼油提高了绵羊体组织cis-9,trans-11-CLA和trans-11-C18:1含量。亚麻籽日粮中添加2mg Se/kg DM的有机硒对绵羊体组织脂肪酸组成没有显著影响。鱼油与有机硒的添加对绵羊不同组织△9-脱氢酶活性各组间影响差异不显著。由此表明,日粮中联合添加亚麻籽+鱼油以提高组织CLA含量的效果优于单独添加亚麻籽。  相似文献   

17.
本文旨在研究饲粮中添加黄花蒿乙醇提取物对奶牛产奶性能及乳脂中共轭亚油酸(CLA)含量的影响。试验选取体重(600±29)kg、胎次2~3胎、泌乳期(158±3)d及泌乳量(22.8±1.8)kg/d的泌乳期荷斯坦奶牛15头,采用完全随机区组设计分为3组,每组5头,进行为期40 d的饲养试验,其中1~9 d为预试期,10~40 d为正试期。对照组饲喂基础饲粮,试验1组和试验2组分别在基础饲粮中添加96和160 g/(d·头)的黄花蒿乙醇提取物。结果表明:整个试验期间,2个试验组的产奶量(第24、31和40天)、乳脂率、乳脂产量、乳蛋白率、乳蛋白产量、非脂固形物含量以及乳脂中的饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸、短链脂肪酸、中链脂肪酸和长链脂肪酸含量与对照组相比均无显著差异(P0.05);2个试验组的乳脂中CLA含量分别比对照组提高了30.7%和38.6%,其中试验2组与对照组差异显著(P0.05)。由此得出,饲粮中添加适量的黄花蒿乙醇提取物对奶牛产奶性能没有明显影响,但可以提高其乳脂中CLA含量,从而优化乳脂中脂肪酸的比例。  相似文献   

18.
In the current study, we hypothesized that diets high in linoleic acid would increase conjugated linoleic acid (CLA) tissue content, reduce adiposity and leptin production, and result in an increase in the age at puberty in heifers. Heifers were weaned and blocked by body weight (heavy, n = 10, and light, n = 10) and allocated randomly within block to receive isocaloric and isonitrogenous diets with either added fat (HF, n = 10) or no added fat (C, n = 10) from 4 mo of age until post-pubertal slaughter. Whole sunflower seed (55% oil; 70% linoleic acid) was used as the fat source in HF diets and provided 5% added fat from the start of the study until heifers weighed 250 +/- 8 kg, at which time added fat was increased to 7% of dry matter until slaughter. Body weights were recorded weekly, and blood samples were collected weekly for total cholesterol and hormone analyses. Puberty was confirmed based on serum concentrations of progesterone and ultrasonographic confirmation of corpora lutea. Heifers were slaughtered at 325 +/- 10 d of age, and longissimus muscle between the 9th and 11th rib was collected and analyzed to estimate carcass composition. Subcutaneous and kidney, pelvic, and heart fat were collected at slaughter for fatty acid analyses. The HF heavy group tended (P < 0.10) to reach puberty later than all other groups, and one HF light heifer did not reach puberty during the study. Linoleic acid and cis-9, trans-11 CLA tissue contents were higher (P < 0.03) in HF heifers than controls, but neither total carcass fat nor percentage of dry matter differed by dietary group, although the percentage of protein tended (P < 0.10) to be lower in HF heifers. Mean serum concentrations of leptin did not differ due to diet; however, leptin increased (P < 0.01) linearly as puberty approached. Circulating concentrations of growth hormone and insulin-like growth factor I increased or remained relatively constant between wk 2 to 10 of feeding, and then declined (P < 0.01) until the onset of puberty. Serum IGF-I was lower (P < 0.01) in heifers receiving the HF diet. Mean serum concentrations of insulin and total cholesterol increased (P < 0.01) with time in both groups, but only total cholesterol was increased by the HF diet (P < 0.05). Results indicate that diets high in linoleic acid fed to growing beef heifers beginning early in life have little or no effect on total carcass fat, circulating leptin, or age at puberty despite measurable increases in CLA accumulation.  相似文献   

19.
Comparisons were made of the performance and carcase composition of lean and fat broilers from the 4th generation of a breeding experiment which were fed diets containing 25 or 80 g fat/kg. Selection over the 4th generation resulted in continued divergence in the selection trait and the correlated responses of total body lipid and protein contents and the efficiencies of conversion of food and dietary protein. In both lines body weight, efficiency of food utilisation and total body lipid and protein contents were unaffected by dietary fat content. Tissue fatty acid composition was influenced by dietary and genetic factors: dietary fat increased the proportions of linoleic and oleic acids at the expense of palmitic, palmitoleic and stearic acids whilst greater body fatness increased the proportion of palmitoleic at the expense of linoleic acid.  相似文献   

20.
An experiment was conducted to determine the efficacy of dietary betaine, CLA, or both as growth promotants and carcass modifiers in growing Iberian pigs. Twenty gilts (20 kg of BW) were individually penned and fed barley- and soybean meal-based diets (12% CP, 0.81% Lys, and 14.8 MJ of ME/kg of DM) containing either no added betaine or CLA (control), 0.5% betaine, 1% CLA, or 0.5% betaine + 1% CLA, at 95% of ad libitum energy intake. An additional group of 5 pigs was slaughtered at the beginning of the experiment to obtain the initial body composition. At 30 kg of BW, a balance experiment was conducted. At 50 kg of BW, pigs were slaughtered and viscera was removed and weighed. Betaine or CLA alone did not affect growth performance. However, betaine + CLA increased ADG (601 vs. 558 g, P = 0.03) and gain relative to ME intake (25.4 vs. 22.2 g/MJ, P = 0.03) compared with control pigs. Digestibility of nutrients and metabolizability of energy did not differ among diets (P = 0.46 to 0.75). Carcass protein, water, and lean deposition (g/d) increased (19.8, 24.2, and 23.4%, respectively, P < 0.01) in pigs fed betaine + CLA compared with control pigs. Similarly, protein deposition relative to ME intake increased by 28% in betaine + CLA-supplemented pigs (P < 0.05). Fat and mineral deposition did not differ among treatments. Carcass protein, water, and lean content (g/kg of carcass) of pigs fed betaine + CLA-supplemented diets tended to increase (P = 0.07 to 0.09) and carcass fat content tended to decrease (P = 0.09). Similarly, estimated composition of carcass gain was affected, such that water and lean content tended to increase (P = 0.06 to 0.08), whereas fat tended to decrease (P = 0.08) in pigs fed betaine + CLA-supplemented diets. Longissimus muscle area was not altered by treatments (P = 0.49). The liver of pigs fed betaine + CLA diets had increased weight (19%, P < 0.05) compared with control pigs. Overall, dietary supplementation of betaine + CLA increased ADG, protein, water, and lean deposition in growing Iberian gilts. There appears to be a synergistic action when betaine and CLA are used together.  相似文献   

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