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1.
以花粉发芽率作为花粉生活力的指标,培养基用15%蔗糖, 1%琼脂加0.01%硼酸配制而成,在25℃恒温室中培养,并观察了17个品种,每品种3次重复,每次重复观察3个视野.结果:观察品种中发芽率最高者为前坪 1#,达 68.2%,其次为桃李,达 53. 6%,最低为姜黄李,发芽率为0;其余大部分品种花粉发芽率低于4%,占所测品种的76%左右。由此说明李大多数品种花粉生活力均差,这可能是李杂交结实率低的原因之一。 花粉贮藏供试品种为前坪1#、酥李和桃李3个品种,设温度、湿度、光三个因素,共8个处理。结果表明:温度是影响李花粉贮藏的主要因素,以低温干燥保藏效果最好… 相似文献
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1997年 ,我站引进日本桃系列品种 ,1 998年建园定植 ,通过几年管理 ,2 0 0 1年少量挂果 ,2 0 0 2年全部结果 ,对品种特性进行了初步观察 ,现将试验结果报道如下。1 基本特性1 .1 物候期日本桃各品种萌芽、抽梢基本一致 ,无明显差异。开花时间 ( 2 0 0 2年 3月 1 8日 )基本一致 ,白桃、浅间白桃开花晚 1天 ,整个花期长 7天左右。落叶期均为 1 0月中、下旬。1 .2 各品种花粉情况 白桃、朝仓早生、浮羽早生、浅间白桃及国内源东白桃、砂子早生均无花粉 ,千曲白凤、八幡白凤、红清水、黄金桃、早熟黄金桃、武井白凤、日川白凤、农林 1 4号花… 相似文献
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草莓新品种宁玉的花粉特性研究 总被引：2，自引：0，他引：2
《中国果树》2014,(5)
为探明草莓新品种宁玉的花粉特性,以宁玉、红颜2个品种为试材,研究不同花期的花粉生活力与不同培养基成分、不同温度、不同光质对花粉生活力的影响。结果表明:2个品种在刚开花期的花粉生活力均达到最高,其中宁玉显著高于红颜,花粉萌发率分别为91.11%、72.94%;宁玉每枚花药中花粉数量显著高于红颜,畸形花粉率显著低于红颜;10%15%蔗糖+0.10%硼酸为2个品种的最适花粉培养基;温度为25℃时2个品种花粉萌发率均达到最高;在5℃下处理8 h后宁玉花粉萌发率48.53%,而红颜仅28.08%;在45℃下处理8 h后宁玉花粉仍有少量萌发,而红颜未萌发;不同光质对2个品种的花粉生活力影响不显著。由此可见,宁玉花粉生活力高于红颜,具有更好的耐低温、耐高温性,推广价值更高。 相似文献
4.
不同保存温度对几个梨品种花粉生活力的影响 总被引：2，自引：0，他引：2
对5个梨品种的花粉进行了不同温度条件下的保存试验.结果表明:保存前花粉发芽率较高,随着室温保存时间的延长,花粉的发芽率迅速下降.60 d后,各品种梨的花粉发芽率降低到10%以下.红酥脆、满天红、黄金梨、奥冠红梨和台湾雪梨分别降为3.8%、3.7%、4.6%、5.8%、5.1%.因此,室温长期保存花粉效果较差.但若进行短时间(5～10 d)贮藏,其保存效果还是比较理想的.低温保存条件下,随着保存时间的延长,各品种梨花粉的生活力下降不太明显,4 ℃下和-20℃下保存60 d后,花粉的生活力仍然在74%和78%以上,而-20℃下保存的花粉生活力较4℃稍强,因此,在-20℃下较长时间保存梨花粉效果更为理想. 相似文献
5.
紫荆花粉生活力测定及贮藏方法研究 总被引：1，自引：0，他引：1
以紫荆花粉为试材,利用离体培养法、碘-碘化钾染色法(I2-KI)、醋酸洋红染色法和氯化三苯基四氮唑(TTC)染色法对花粉生活力进行测定,并研究了不同贮藏条件对花粉萌发的影响,以期了解紫荆花粉的萌发特性及适宜的贮藏方法。结果表明:培养基中添加不同浓度的蔗糖和硼酸对花粉的萌发有促进作用,紫荆花粉萌发的最适培养基为15%蔗糖+0.004%硼酸+0.08%的琼脂,萌发率达88.46%;I2-KI染色法和TTC染色法不适用于测定紫荆花粉生活力;而醋酸洋红染色法可以作为测定紫荆花粉生活力的方法;花粉生活力随贮藏时间的延长而下降,最佳贮藏方法为4℃干燥,耐贮藏力超过26d。 相似文献
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本试验以毛桃、白凤、85007、穆阳水蜜桃早熟种、穆阳水蜜桃中熟种和穆阳水蜜桃晚熟种等6个品种（系）3种开放程度花朵的花粉为试验材料,进行花粉萌发力及花粉管生长试验。结果表明：供试的毛桃、白凤和85007 3个品种3种发育程度的花粉其萌发率达极显著差异,全开放〉半开放〉未开放。穆阳水蜜桃中熟种、晚熟种全开放与半开放没有显著差异,与未开放有显著差异;早熟种全开放、半开放、未开放间没有显著差异。供试6个品种花粉萌发力高低的顺序为毛桃〉晚熟种〉中熟种〉白凤〉85007〉早熟种,花粉萌发率分别为71%、56.2%、54.3%、37.4%、36.5%、10.5%。穆阳水蜜桃低产树的雄花败育,没有花粉。试验表明,桃花粉萌发、花粉管生长与温度关系密切,随着温度升高花粉萌发率增大,萌发速度加快,花粉管生长也加快。10℃以下花粉萌发率低,萌发慢。 相似文献
10.
新疆野生蔬菜实葶葱种子萌发条件的研究 总被引：1，自引：0，他引：1
以野生实葶葱种子为试材,分别设5、10、15、20、25、30℃6种不同的恒温条件处理,以0℃为对照,研究不同温度条件对实葶葱种子萌发的影响。结果表明:当发芽温度为0、5、10℃时实葶葱种子不能发芽,发芽率为0%;15℃时发芽率为32.67%;在20℃温度下发芽率、发芽势、发芽指数最高,发芽率为75.33%;随着温度条件的升高,实葶葱种子的发芽速度减慢,发芽率呈下降趋势,25℃和30℃的发芽能力低于20℃;因此,实葶葱种子发芽的最适发芽温度为20℃。 相似文献
11.
AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
12.
AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration. 相似文献
13.
《International Journal of Fruit Science》2013,13(3):101-120
Abstract Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared. 相似文献
14.
多效唑对猕猴桃离体试管苗生长及内源激素的影响 总被引：18，自引：0，他引：18
多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。 相似文献
15.
HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献
16.
The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species. 相似文献
17.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation. 相似文献
18.
Duncan Brean W. Boyle Shannon Breininger David R. Schmalzer Paul A. 《Landscape Ecology》1999,14(3):291-309
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere. 相似文献
19.
AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis. 相似文献
20.
Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations
from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations,
dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture
or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near
Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between
November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation
was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme
temperatures (summer maximums, winter minimums). The correlation (r
2) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation
in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences
respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds
that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for
37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest
species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain
the patchy distribution of many species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献