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1.
The feasibility of using planted biofilters for purification of recirculated aquaculture water in the Mekong Delta of Vietnam was assessed. The plant trenches were able to clean tilapia aquaculture water and to maintain good water quality in the fish tanks without renewal of the water. NH4‐N was removed efficiently in the plant trenches, particularly in the trenches with Canna glauca L., probably because of plant uptake and nitrification–denitrification. Plant uptake constituted 6% of N and 7% of P in the input feed. Approximately 1.0 m3 of water was needed per kg of fish produced, and 370, 97 and 2842 g fresh aboveground biomass of Ipomoea aquatica Forssk., Lactuca sativa L. and C. glauca, respectively, were produced. The leafy vegetables provide some extra income besides fish products, whereas C. glauca provides nice flowers and contributes to a significant nutrient removal with annual uptake rates of 725 kg N and 234 kg P ha?1 year?1. This research demonstrates that integrated recirculating aquaculture‐hydroponics (aquaponics) systems provide significant water savings and nutrient recycling as compared with traditional fish ponds.  相似文献   

2.
During a 1-year operation of a warm water recycling system (salinity about 8‰) sufficient water quality was maintained under high stocking density conditions using Tilapia and the European eel as potential candidates for intensive farming. The final fish: water ratio was 1 : 23 (or 43.5 g fish per 1 water) for the whole experimental culture unit. The total water volume of the system was about 5 m3. The water treatment unit held 52% of the total volume, whereby 46% was available for fish culture. Combined biological (trickling filter with Hydropack-foil) and chemical (ozonation) water treatment proved to be useful to meet water quality requirements under these rearing conditions. After an initial conditioning period of the biofilter, BOD varied from 4.5 to 6.0 mg O2/l, ammonium levels were maintained at less than 1 mg/l and nitrite concentrations averaged 1 mg/l. The average efficiency (oxidation rate) of the biofilter for NH4+-and NO2?-oxidation was 31% and 13.2%, respectively. The pH was stabilized slightly above 7.0 when a denitrification unit was connected to the system. Nitrate concentration of the system levelled of between 200 and 400 mg/l and was regulated by the addition of an electron donator (first glucose solution, then methanol) to the denitrification unit; the elimination rate averaged 50% with a maximum of 98%. High nitrite levels were avoided by ozone treatment of the recycled water. The accumulation of low-biodegradable substances was also successfully counteracted by ozonation. Fish growth rates of about 30% per month at high stocking densiteis were reached for Tilapia at a fish: water ratio of 1 : 4.6 (217 g fish per 1 water), indicating that a combination of biological water treatment and ozonation supports intensive fish culture in a closed aquaculture system.  相似文献   

3.
Intensive recirculating aquaculture relies on biofilters to sustain satisfactory water quality in the system. Fluidized bed and immobilized cell technologies were used to remove ammonia from the water and maintain fish health. A high‐rate nitrifying fluidized bed biofilter combined with valveless filter was designed for use in a recirculation aquaculture system (RAS). The suspended solids produced during fish culture could automatically be removed using a valveless filter. Natural porosity with fitting proportion, steady fluidization and expanding rate was chosen as the fluidized carrier. The technology of bacterial separation and cultivation was used. The immobilized Rhodopseudomonas palustris (R. palustris) produced through a biotechnologically embedding medium is suitable for fish and could help prevent diseases. Nitrification was promoted through the selective rearing of nitrobacteria in a fluidized bed biofilter. Water quality was improved using fluidized bed biofilter and immobilized R. palustris in the RAS. In addition, the proposed system was able to reduce costs. Maximum fish load was 45 ± 3 kg m?3 in the closed recirculating water fish culture system, and water use was reduced by 80–90%. The total ammonia nitrogen removal rate of the technology was 80–95%, and nitrite N removal rate was above 80%.  相似文献   

4.
Aerobic biological filtration systems employing nitrifying bacteria to remediate excess ammonia and nitrite concentrations are common components of recirculating aquaculture systems (RAS). However, significant water exchange may still be necessary to reduce nitrate concentrations to acceptable levels unless denitrification systems are included in the RAS design. This study evaluated the design of a full scale denitrification reactor in a commercial culture RAS application. Four carbon sources were evaluated including methanol, acetic acid, molasses and Cerelose™, a hydrolyzed starch, to determine their applicability under commercial culture conditions and to determine if any of these carbon sources encouraged the production of two common “off-flavor” compounds, 2-methyisoborneol (MIB) or geosmin. The denitrification design consisted of a 1.89 m3 covered conical bottom polyethylene tank containing 1.0 m3 media through which water up-flowed at a rate of 10 lpm. A commercial aquaculture system housing 6 metric tonnes of Siberian sturgeon was used to generate nitrate through nitrification in a moving bed biological filter. All four carbon sources were able to effectively reduce nitrate to near zero concentrations from influent concentrations ranging from 11 to 57 mg/l NO3–N, and the maximum daily denitrification rate was 670–680 g nitrogen removed/m3 media/day, regardless of the carbon source. Although nitrite production was not a problem once the reactors achieved a constant effluent nitrate, ammonia production was a significant problem for units fed molasses and to a less extent Cerelose™. Maximum measured ammonia concentrations in the reactor effluents for methanol, vinegar, Cerelose™ and molasses were 1.62 ± 0.10, 2.83 ± 0.17, 4.55 ± 0.45 and 5.25 ± 1.26 mg/l NH3–N, respectively. Turbidity production was significantly increased in reactors fed molasses and to a less extent Cerelose™. Concentrations of geosmin and MIB were not significantly increased in any of the denitrification reactors, regardless of carbon source. Because of its very low cost compared to the other sources tested, molasses may be an attractive carbon source for denitrification if issues of ammonia production, turbidity and foaming can be resolved.  相似文献   

5.
Comparative studies of larviculture of Macrobrachium rosenbergii (de Man) were made for three systems: (1) the static water system with partial changes of water, (2) the closed recirculating water system with a separate subsand filter unit, and (3) the closed recirculating water system with a subsand filter inside the rearing tank. The mean production of juveniles per unit volume of water of these three systems was comparable (3.2, 1.9, and 2.9, respectively). Production fluctuated more in the static system than in the closed recirculating system. A significant asymptotic relationship between the stocking density and the survival of prawn larvae was observed in the first type of the closed recirculating system; higher stocking density resulted in lower survival. A stocking density of 20 larvae/l in the closed recirculating system was considered to be good for growth and survival. The intermittent high nitrite level was a problem encountered in the static system but not in the closed recirculating system. The operational costs of these three systems were discussed.  相似文献   

6.
Photosynthetic suspended-growth systems in aquaculture   总被引:3,自引:0,他引:3  
Standardized evaluation and rating of biofilters for aquaculture should be assessed in the context of the economic efficiency of ecological services (waste assimilation, nutrient recycling, and internal food production) provided by earthen ponds, and the availability and cost of land, water, and electrical energy resources required to support particular classes of production systems. In photosynthetic suspended-growth systems, water quality control is achieved by a combination of natural and mechanical processes. Natural processes include photosynthesis of oxygen, algal nutrient uptake, coupled nitrification–denitrification, and organic matter oxidation; mechanical processes include aeration and water circulation. Ammonia is controlled by a combination of phytoplankton uptake, nitrification, and immobilization by bacteria. Unlike biofilters for recirculating aquaculture systems, unit processes are combined and are an integral part of the culture unit. The important design and operational considerations for photosynthetic suspended-growth systems include temperature effects, aeration and mixing, quantity and quality of loaded organic matter, and fish water quality tolerance limits. The principle advantages of photosynthetic suspended-growth systems are lower capital costs relative to other recirculating aquaculture systems and increased control over stock management relative to conventional static ponds. The main disadvantage is the relatively low degree of control over water quality and phytoplankton density, metabolism, and community composition relative to other recirculating aquaculture systems. Examples of photosynthetic suspended-growth systems include semi-intensive ponds, intensively aerated outdoor lined ponds, combined intensive–extensive ponds, partitioned aquaculture systems, greenwater tanks, greenwater tanks with solids removal, and greenwater recirculating aquaculture systems.  相似文献   

7.
Use of live and dead probiotic cells in tilapia Oreochromis niloticus   总被引:3,自引:0,他引:3  
ABSTRACT:   To investigate the effect of live and dead probiotic cells on the non-specific immune system of tilapia Oreochromis niloticus , probiotics were introduced by feeding either in the form of live or dead cells, or supplying live cells to the rearing water in a closed recirculating system. The probiotics treatment enhanced non-specific immune parameters such as lysozyme activity, migration of neutrophils and plasma bactericidal activity, resulting in improvement of resistance to Edwardsiella tarda infection. Especially, oral administration of live cells seemed to be more effective compared with other probiotic treatments such as oral administration of dead probiotic cells and supply of live probiotic cells to the rearing water. These results indicate that probiotics treatment is promising as an alternative method to antibiotics for disease prevention in aquaculture, and the viability of probiotic bacteria is a key factor to induce more potential effect of probiotics used for fish production.  相似文献   

8.
Competition between heterotrophic and nitrifying bacteria is of major practical importance in aquaculture biofilter design and operation. This competition must be understood to minimize the negative impact of heterotrophic bacteria on an aquaculture system. On the other hand, the heterotrophic population is suspected of having a positive effect against pathogenic bacteria. Little information is available on the bacterial communities present within aquaculture systems, except for nitrifying bacteria, but a combination of traditional aquacultural engineering research methods and novel microbiological techniques offers new opportunities for the study of these communities.

The heterotrophic bacterial population activity and the nitrification efficiency of a submerged biological filter were studied for an influent TAN concentration of 2 mg/l and varying C/N ratios. The TAN removal rate was found to be 30% lower at a C/N ratio of 0.5 than at a C/N ratio of 0. For higher C/N ratios the reduction in nitrification efficiency was 50% while the attached bacterial abundance was doubled. Moreover, results confirm that abundance of sheared and attached bacteria are correlated. It is not known to what extent biofilter configuration might influence the relationship between heterotrophic and nitrifying bacteria, and further work will be carried out with moving bed and fluidized filters. A better understanding of the role of the heterotrophic bacteria in RAS will help to optimize any positive “biocontrol” effect and to minimize the microbial degradation of rearing water and the reduction of nitrification rates.  相似文献   


9.
This paper presents an innovative process to solve the nitrate build-up problem in recirculating aquaculture systems (RAS). The novel aspects of the process lie in a denitrification bioreactor system that uses solid cotton wool as the primary carbon source and a unique degassing chamber. In the latter, the water is physically stripped of dissolved gaseous O2 (by means of a Venturi vacuum tube), and the subsequent denitrification becomes more efficient due to elimination of the problems of oxygen inhibition of denitrification and aerobic consumption of cotton wool. The cotton wool medium also serves as a physical barrier that traps organic particles, which, in turn, act as an additional carbon source for denitrification. Operation in the proposed system gives an extremely low C/N ratio of 0.82 g of cotton wool/g of nitrate N, which contributes to a significant reduction of biofilter volume. The additional advantage of using solid cotton wool as the carbon source is that it does not release organic residuals into the liquid to be recycled. Operation of the system over a long period consistently produced effluents with low nitrate levels (below 10 mg N/l), and there was only a very small need to replace system water. The overall treatment scheme, also incorporating an aerobic nitrification biofilter and a granular filtration device, produced water of excellent quality, i.e., with near-zero levels of nitrite and ammonia, a sufficiently high pH for aquaculture, and low turbidity. The proposed system thus provides a solution for sustainable small-scale, urban aquaculture operation with a very high recovery of water (over 99%) and minimal waste disposal.  相似文献   

10.
To achieve water reuse in recirculating aquaculture systems, intermittent nitrification and denitrification processes using internal fibrous media was proposed. A pre-acclimated Biocord biofilter, with an initial nitrification rate of 17.1 ± 12.4 mg total ammonia nitrogen-N/m2/d was applied in a marine whiteleg shrimp (Litopenaeus vannamei) culture tank. Throughout the experiment, the aerobic nitrification activity of the biofilter was sufficient to control the ammonia and nitrite levels below 0.2 mg-N/L with an accumulation of nitrate up to 50 mg-N/L. The remaining nitrate was successfully removed after shrimp harvest with the same biofilter through anoxic denitrification in conjunction with a methanol supplement at a chemical oxygen demand: nitrate-N ratio of 5:1. With complete nitrogen removal, the water was re-aerated and the next crop of shrimp culture was initiated. In this study, a two-crop shrimp cultivation was performed in sequence in the same tank without water exchange. The microbial diversity was monitored using high-throughput sequencing on Illumina MiSeq, which demonstrated that Proteobacteria (45.3 %), Chloroflexi (18.4 %), and Bacteroidetes (17.1 %) were the most abundant phyla. With an emphasis on nitrogen removal, the family Nitrosomonadaceae and Nitrospiraceae were the dominant nitrifying bacteria during the aerobic nitrification, while a high relative abundance of the Methylophaga and Methylotenera genera was observed under the anoxic condition.  相似文献   

11.
The development of marine intensive land‐based aquaculture systems has been limited due to the absence of methods to manage saline wastewater. Aquaponic systems, although commonly applied to freshwater aquaculture, can potentially manage nutrient wastes while providing a secondary product. The aim of this study was to evaluate both the capacity for water treatment and the production requirements of two saltwater‐tolerant plant species (Sesuvium portulacastrum and Batis maritima) when grown hydroponically in a marine aquaponic system. The presence of plants was found to significantly contribute to nitrate removal, such that mean nitrate concentrations were 10.1 ± 5.4 and 12.1 ± 6.1 mg/L NO3?‐N in planted and unplanted treatments respectively. The use of coconut fibre as a planting medium also significantly contributed to nitrate removal, such that mean nitrate concentrations were 9.78 ± 5.4 and 12.4 ± 6.0 mg/L NO3?‐N in coconut fibre and expanded clay treatments respectively. Daily nitrogen removal was greatest in the coconut fibre/plants treatment, ranging from ?18% to 67%. Hydraulic loading rate, plant species and plant density did not significantly affect water quality or plant growth. The low flow/saltwort/low density treatment had the greatest mean daily nitrogen removal, ranging from 25% to 172%. The results indicate that the main nitrogen removal mechanisms were simultaneous nitrification–denitrification in the hydroponic plant beds and nitrogen removal through plant growth. This study demonstrates that marine aquaponics could be an effective way to manage nutrient removal in marine land‐based aquaculture systems.  相似文献   

12.
Total ammonia nitrogen (TAN) concentration is often a key limiting water quality parameter in intensive aquaculture systems. Removing ammonia through biological filtration is thus the first objective in recirculating aquaculture system design. In this study, the performance characteristics of a steady-state nitrification biofilm were explored using a series of reactors. Four nitrification kinetics parameters were estimated using the data collected from the experimental system, including minimum TAN concentration, half saturation constant, maximum TAN removal rate and maximum specific bacterial growth rate. Experimental data showed that a minimum TAN concentration was needed to support a steady-state nitrification biofilm. For the temperature of 27.2°C, the mean minimum TAN concentration was 0.07 mg/l. For a single substrate-limiting factor, the relationship between TAN removal rate (R) and TAN concentration (S) was represented by an empirical equation [R=1859(S−0.07)/(S+1.93)]. The characteristics of nitrite oxidation were also demonstrated by the experiment system. The results of this study will help to better understand the characteristics of nitrification biofilters applied in recirculating aquaculture systems.  相似文献   

13.
This study evaluated wood chips and wheat straw as inexpensive and readily available alternatives to more expensive plastic media for denitrification processes in treating aquaculture wastewaters or other high nitrate waters. Nine 3.8-L laboratory scale reactors (40 cm packed height × 10 cm diameter) were used to compare the performance of wood chips, wheat straw, and Kaldnes plastic media in the removal of nitrate from synthetic aquaculture wastewater. These upflow bioreactors were loaded at a constant flow rate and three influent NO3–N concentrations of 50, 120, and 200 mg/L each for at least 4 weeks, in sequence. These experiments showed that both wood chips and wheat straw produced comparable denitrification rates to the Kaldnes plastic media. As much as 99% of nitrate was removed from the wastewater of 200 mg NO3–N/L influent concentration. Pseudo-steady state denitrification rates for 200 mg NO3–N/L influent concentrations averaged (1360 ± 40) g N/(m3 d) for wood chips, (1360 ± 80) g N/(m3 d) for wheat straw, and (1330 ± 70) g N/(m3 d) for Kaldnes media. These values were not the maximum potential of the reactors as nitrate profiles up through the reactors indicated that nitrate reductions in the lower half of the reactors were more than double the averages for the whole reactor. COD consumption per unit of NO3–N removed was highest with the Kaldnes media (3.41–3.95) compared to wood chips (3.34–3.64) and wheat straw (3.26–3.46). Effluent ammonia concentrations were near zero while nitrites were around 2.0 mg NO2–N/L for all reactor types and loading rates. During the denitrification process, alkalinity and pH increased while the oxidation–reduction potential decreased with nitrate removal.

Wood chips and wheat straw lost 16.2% and 37.7% of their masses, respectively, during the 140-day experiment. There were signs of physical degradation that included discoloration and structural transformation. The carbon to nitrogen ratio of the media also decreased. Both wood chips and wheat straw can be used as filter media for biological denitrification, but time limitations for the life of both materials must be considered.  相似文献   


14.
Denitrification in recirculating systems: Theory and applications   总被引:20,自引:0,他引:20  
Profitability of recirculating systems depends in part on the ability to manage nutrient wastes. Nitrogenous wastes in these systems can be eliminated through nitrifying and denitrifying biofilters. While nitrifying filters are incorporated in most recirculating systems according to well-established protocols, denitrifying filters are still under development. By means of denitrification, oxidized inorganic nitrogen compounds, such as nitrite and nitrate are reduced to elemental nitrogen (N2). The process is conducted by facultative anaerobic microorganisms with electron donors derived from either organic (heterotrophic denitrification) or inorganic sources (autotrophic denitrification). In recirculating systems and traditional wastewater treatment plants, heterotrophic denitrification often is applied using external electron and carbon donors (e.g. carbohydrates, organic alcohols) or endogenous organic donors originating from the waste. In addition to nitrate removal, denitrifying organisms are associated with other processes relevant to water quality control in aquaculture systems. Denitrification raises the alkalinity and, hence, replenishes some of the inorganic carbon lost through nitrification. Organic carbon discharge from recirculating systems is reduced when endogenous carbon sources originating from the fish waste are used to fuel denitrification. In addition to the carbon cycle, denitrifiers also are associated with sulfur and phosphorus cycles in recirculating systems. Orthophosphate uptake by some denitrifiers takes place in excess of their metabolic requirements and may result in a considerable reduction of orthophosphate from the culture water. Finally, autotrophic denitrifiers may prevent the accumulation of toxic sulfide resulting from sulfate reduction in marine recirculating systems. Information on nitrate removal in recirculating systems is limited to studies with small-scale experimental systems. Packed bed reactors supplemented with external carbon sources are used most widely for nitrate removal in these systems. Although studies on the application of denitrification in freshwater and marine recirculating systems were initiated some thirty years ago, a unifying concept for the design and operation of denitrifying biofilters in recirculating systems is lacking.  相似文献   

15.
单级生物接触氧化法去除海水养殖废水中的无机氮   总被引:1,自引:0,他引:1  
利用在填料上人工接种微生物组成的浸没式生物接触氧化单级处理系统对养殖废水进行净化,效果良好。在试验水体体积与处理系统体积之比约为100∶1的情况下,对氨氮、亚硝酸盐氮、硝酸盐氮起始质量浓度分别为4.0 mg/L、1.76 mg/L、800 mg/L,COD质量浓度为16.33 mg/L的养殖废水进行处理,发现处理系统中进行着强烈的硝化和反硝化作用:处理30 h,氨氮质量浓度下降并一直保持在0.1 mg/L;亚硝酸盐氮浓度48 h内,前6 h从1.76 mg/L短暂上升到2.24 mg/L,然后持续下降,最低到0.22 mg/L;对硝酸盐氮的反硝化作用能力也很强,经48 h处理,硝酸盐氮质量浓度从800 mg/L下降到180 mg/L。根据对处理过程中的水质测定,浸没式生物接触氧化单级处理试验系统具有较强的生物脱氮能力。  相似文献   

16.
海水贝类苗种循环水培育水处理技术的研究与应用   总被引:3,自引:0,他引:3  
刘鹰  郑瑞东 《水产科学》2005,24(12):21-24
封闭循环水育苗作为一种高技术育苗模式,其核心是养殖废水的净化处理。对于循环水系统的每一道处理工序,都有大量可选择的工艺。本试验利用自建的100 m3的海湾扇贝苗种封闭循环水培育系统,通过用各个阶段的出水养殖扇贝幼体,以及测定出水指标等来判断水处理系统的处理效果。结果表明,封闭循环水处理系统的效果是成功的。  相似文献   

17.
Reduced fishery harvests and increased consumer demand for seafood have precipitated an increase in intensive fish farming, predominantly in coastal and open ocean net-pens. However, as currently practiced, aquaculture is widely viewed as detrimental to the environment and typical operations are vulnerable to environmental influences, including pollution and endemic diseases. Here we report the development of a land-based, marine recirculating aquaculture system that is fully contained, with virtually no environmental impact as a result of highly efficient biological waste treatment and water recycling. Over 99% of the water volume was recycled daily by integrating aerobic nitrification to eliminate toxic ammonia and, for the first time, simultaneous, anaerobic denitrification and anaerobic ammonium oxidation, to convert ammonia and nitrate to nitrogen gas. Hydrogen sulfide generated by the separated endogenous organic solids was used as an electron source for nitrate reduction via autotrophic denitrification and the remaining organic solids were converted to methane and carbon dioxide. System viability was validated by growing gilthead seabream (Sparus aurata) from 61 g to 412 g for a total of 1.7 tons in a record 131 days with 99% fish survival. Ammonia nitrite and nitrate did not exceed an average daily concentration of 0.8 mg/l, 0.2 mg/l and 150 mg/l, respectively. Food conversion values were 16% lower than recorded levels for net-pen aquaculture and saltwater usage of less than 16 l/every kg of fish produced. The system is site-independent, biosecure, devoid of environmental contaminants and is not restricted to a single species.  相似文献   

18.
Nitrification kinetics of biofilm as affected by water quality factors   总被引:12,自引:0,他引:12  
Various types of fixed film biofilters have been used in recirculating aquaculture systems under different water quality and operating conditions. The effectiveness of the nitrification process can be evaluated by nitrification kinetics. Nitrification in the bacterial film of the biofilter involves physical, chemical and biological processes that are governed by a variety of parameters such as substrate and dissolved oxygen concentrations, organic matters, temperature, pH, alkalinity, salinity and turbulence level. The impacts of these parameters upon nitrification kinetics make predicting the performance of a biofilter for a given application an engineering challenge. Knowing the performance of a biofilter is critical for both designers and managers. This paper summarizes the current knowledge on nitrification kinetics as affected by the aforementioned factors based on literature and the results from the authors’ laboratories. These factors were ranked according to their significance of impact on biofilter nitrification performance. The information presented can be used as a reference for the design and operation of biofilters in recirculating aquaculture systems.  相似文献   

19.
为了实现规模化人工养殖小丑鱼(Amphiprioninae),研发了小丑鱼室内循环水养殖设施和技术。1组循环水养殖系统由10个玻璃钢养殖桶和1个水处理玻璃缸及管道系统组成,采用物理过滤、生化过滤、藻板过滤进行循环水处理。1组循环水养殖系统每3个月可养殖产出全长约3.5 cm的商品小丑鱼5 000尾,养殖存活率达80%以上。从2014年至2015年,利用该设施养殖生产出商品小丑鱼10余万尾。和常规的食用海水鱼循环水养殖设施相比,小丑鱼室内循环水养殖系统主要减少了蛋白分离器、气浮机、微滤机等设备,增加了藻板过滤设施。研究表明,小丑鱼室内循环水养殖系统建造成本低、运行能耗低、管理维护简单、水质稳定,可基本实现全封闭循环水养殖,适合进行小丑鱼等海水珊瑚礁观赏鱼类的规模化养殖生产。  相似文献   

20.
Fish Physiology and Biochemistry - The nitrification process in recirculating aquaculture systems can reduce water pH. Fish can also be exposed to water acidification during transport, an important...  相似文献   

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