共查询到20条相似文献，搜索用时 46 毫秒
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为了给小麦遗传转化研究提供参考,总结和分析了小麦基因转化的最新研究进展,并针对存在的主要问题提出了今后的研究方向.目前在小麦表达载体构建中普遍采用Ubi和在35S基础上改造而来的启动子序列,使用的报告基因主要有gus、uidA、gfp和Cl/LC等,选择基因主要有bar、nptⅡ、manA(pmi)、Cah、gst27、mopat、popat、CP4和GOX,筛选剂一般选用Bialaphos、Glufosinate、G418和Glyphosate等.小麦基因转化的主要受体为幼胚及其愈伤组织,转化方法主要有花粉管通道法、基因枪法和农杆菌介导法.受体类型、表达载体序列组成和基因转化方法是影响小麦基因转化效率的主要因素. 相似文献
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油菜是我国主要油料作物之一．主要分布在我国的长江流域和黄淮流域。黄淮流域主要分布在河南省、陕西省的关中地区、江苏省和安徽省的北部、山西省的南部和甘肃省的东南部等6个省份。 相似文献
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W. L. Porter 《American Journal of Potato Research》1967,44(10):382-382
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Effects of timing and rate of N fertilizer application on concentrations of P, K, S, Ca, Mg, Na, Cl, Mn, Fe, Cu and Zn in herbage from perennial ryegrass/white clover pastures were studied at two sites in south-western Victoria, Australia. Nitrogen fertilizer (0, 15, 25, 30, 45 and 60 kg ha–1 ) was applied as urea in mid-April, early May, mid-May, early June and mid-June 1996 to pastures grazed by dairy cows. At Site 1, N fertilizer resulted in a linear increase in P, K, S, Mg and Cl concentrations in herbage and a linear decrease in Ca concentration. For all times of application, concentrations of P, K, Ca, Mg and Cl in herbage increased by 0·0048, 0·08, −0·010, 0·0013 and 0·053 g kg–1 dry matter (DM) per kg N applied respectively. For S concentration, maximum responses occurred in mid-May (0·012 g kg–1 DM per kg N applied). At Site 2, N fertilizer resulted in a linear increase in P, S and Na concentrations in herbage, a linear decrease in Ca concentration and a curvilinear increase in K and Cl concentration. The maximum responses for P, S and K concentrations in herbage occurred for the N application in mid-June and were 0·015, 0·008 and 0·47 g kg–1 DM per kg N applied respectively. For Cl concentration, the maximum response occurred for the N application in early June and was 0·225 g kg–1 DM per kg N applied. Overall, applications of N fertilizer up to 60 kg ha–1 did not alter herbage mineral concentration to levels that might affect pasture growth or animal health. 相似文献
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Krishna Kumari S Thayumanavan B 《Plant foods for human nutrition (Dordrecht, Netherlands)》1998,53(1):47-56
Scanning electron microscope (SEM) pictures of small millet starch granules showed more large polygonal and few small spherical or polygonal granules. The granules of small millets resembled those of rice starch granules. The size of the starch granules ranged from 0.8–10 m. The size of the granules was larger in barnyard millet and smaller in proso millet. Several granules showed deep indentation caused by protein bodies. SEM of starch isolated from 24 hour-germinated kodo millet showed pitting or pinholes at some points due to the attack of amylases (preferentially on bigger granules). Brabender viscoamylograph studies on small millet starches revealed that the gelatinization temperatures ranged from 75.8 to 84.9 ° C. Barnyard millet possessed lower amylograph viscosity, minimum breakdown, and relative breakdown values when compared to the other small millets. 相似文献