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1.
酶法提取黑木耳多糖的纯化与鉴定   总被引:1,自引:0,他引:1  
利用优化改进的Sevag试剂法对酶法提取的黑木耳粗多糖进行蛋白质的脱除。采用SephadexG-200凝胶层析对黑木耳粗多糖进行分级纯化,得到两个组分AAP1、AAP2,合并收集液,透析、浓缩、冻干得到黑木耳纯多糖,为白色粉末。采用SephroseA-200凝胶层析、紫外光谱扫描对黑木耳纯多糖进行纯度鉴定。SephroseA-200凝胶层析结果表明,多糖的两种组分都为单一洗脱峰;紫外光谱扫描说明多糖中不合有蛋白质、多肽及核酸物质;说明黑木耳多糖的纯度已经达到物理化学的分析标准。红外光谱扫描的结果表明,黑木耳多糖中合有多糖类物质的特征吸收峰。纸层析法对黑木耳纯多糖进行组分分析的结果表明黑木耳多糖由葡萄糖、木糖、半乳糖、甘露糖、阿拉伯糖组成,是一种杂多糖。  相似文献   

2.
黑木耳多糖速食羹加工工艺研究   总被引:2,自引:0,他引:2  
黑龙江生产黑木耳品质优良。研究生产以黑龙江产黑木耳提取的黑木耳多糖和红豆为原料、琼脂为凝胶剂,制备黑木耳多糖速食羹。感官评分作为评价指标。通过单因素和正交试验,对影响黑木耳多糖速食羹感官品质等加工条件进行探究。试验结果表明,黑木耳多糖速食羹的最佳配方为:黑木耳多糖添加量为2.0%,红豆沙添加量为30%,琼脂添加量为1.0%,白砂糖添加量为2.0%。  相似文献   

3.
用黑木耳提取其有效成份,配伍其它营养辅助因子,可制得风味独特口感良好的保健品凝胶颗粒冲溶 饮品,加工过程中采用提取、配浆、造粒喷雾干燥等工艺。产品外观呈疏松轻脆的多孔状颗粒,具有良好的冲 溶性、分散性和稳定性。经试验,黑木耳加工成黑木耳凝胶的料液浓缩比(A)20:1、料液温度(B)46℃、 进风温度(C)148℃、排风温度(D)为103℃。得率和质量的主次因素为D>C>A>B。  相似文献   

4.
以5%、10%、15%、20%、25%、30%、35%、40%、45%、50%黑木耳茵糠代替木屑配制培养基栽培黑木耳,与未加菌糠的常规培养基进行产量、质量比较试验。结果表明:黑木耳菌糠代替部分木屑袋栽黑木耳是可行的,用30%的茵糠替代木屑生产黑木耳为最适宜配比。配方为木屑47%、茵糠30%、麸皮20%、石膏1%、玉米粉1%、豆粉1%,生产出的黑木耳产量高、质量好。  相似文献   

5.
响应面法优化超声波辅助提取黑木耳多糖的工艺研究   总被引:4,自引:0,他引:4  
黑木耳是我国重要的药食兼用真菌,采用超声波辅助提取黑木耳多糖的方法,改进了传统的浸提工艺。研究超声功率、超声时间、液料比及水浴浸提时间等因素对黑木耳多糖提取效果的影响,在单因素基础上,进行响应面分析实验,结果表明,超声波辅助提取黑木耳多糖的最佳工艺条件为:超声功率520W,超声时间13min,液料比132,水浴浸提时间3.1h,黑木耳多糖得率可达16.59%。  相似文献   

6.
以黑木耳、蓝莓果汁、复合凝胶、蔗糖、柠檬酸为主要原料,经过优化工艺条件、正交试验。结果表明,最佳配方为:黑木耳汁25%、蓝莓汁10%、蔗糖28%、复合凝胶0.6%、柠檬酸0.1%。  相似文献   

7.
一种改良的黑木耳多糖提取方法   总被引:4,自引:0,他引:4  
探索一种黑木耳多糖提取方法。方法:采用黑木耳粉用不同的提取剂对黑木耳进行提取。经浸提、过滤浓缩及除蛋白,最后所得沉淀经硫酸-苯酸法证实均为黑木耳多糖。结果:用稀NaOH溶液作提取剂的多糖含量比蒸馏水作提取剂的多糖含量高。结论:采用稀碱液浸提后不但能节省时间而且能减少原材料及试剂的消耗,且提取的多糖含量高。  相似文献   

8.
比较研究了热提取、超声波提取、微波提取3种不同工艺对栀子黄色素提取效果的影响。热浸提正交试验的最佳工艺条件:70%的乙醇(体积分数,下同)提取1.5h,提取温度30℃,料液比1:25(g:mL,下同),产品的色价为66.45。超声波提取正交试验的最佳工艺条件:80%的乙醇提取20min,料液比1:20,产品的色价为87.77。微波提取单因素试验的最佳工艺条件:提取功率800W,用50%乙醇提取30min,提取温度50℃,料液比1:5,色价为64.14。结果表明:热浸提、超声波提取、微波提取3种方法提取栀子黄色素的色价明显不同,超声波提取的效果最好。  相似文献   

9.
以黑木耳品种“延农科1号”为试验材料,马铃薯、黄豆粉、麦麸、木屑、废弃黑木耳菌袋等浸出液为原料,研究对黑木耳菌种培养的影响,筛选黑木耳菌种最佳培养基。结果表明,黑木耳菌种培养的最佳培养基为配方3和配方5。  相似文献   

10.
黑木耳是一种重要的食药用真菌,含有丰富的营养与功能性成分,其中木耳多糖具有降血糖、降血脂、抗氧化、抗血栓形成以及增强机体免疫力等功效。但是木耳多糖的分离制备方法以传统技术为主,提取效率低,周期长,因此现代技术的开发显得尤为重要。本试验以黑木耳为研究对象,采用响应面方法优化高压均质技术制备黑木耳多糖工艺。结果表明:均质时间25 min,均质压力25 MPa,料液比1∶76,黑木耳多糖得率可达(40.33±0.41)%提取效果显著优于传统水提技术。本试验的研究为促进黑木耳及其多糖资源的深度开发利用提供数据参考。  相似文献   

11.

In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads.  相似文献   

12.

The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage.  相似文献   

13.
Contour hedgerows of multipurpose tree species in the sloping tea lands of Sri Lanka are expected to reduce soil erosion and also add significant amounts of plant nutrients to the soil via periodic prunings. The objective of this experiment was to characterize the biomass decomposition pattern and quantify the amount of nutrients added through prunings of six tree species (Calliandra calothyrsus, Senna spectabilis, Euphatorium innulifolium, Flemingia congesta, Gliricidia sepium and Tithonia diversifolia) currently being used in hedgerows associated with tea. Withered leaf and stem prunings (50 g) were enclosed in 2-mm litter bags, placed at 5-cm depth and retrieved after one, three, six, nine and 12 weeks. Loss of initial dry weight, N, P and K was measured. Single exponential decay function adequately described both dry weight and nutrient loss. Tree species differed significantly in their rate of breakdown with decomposition constants (k) varying from 0.0299 to 0.2006 week−1 for leaves and from 0.0225 to 0.0633 week−1 for stems. Gliricidia showed the highest k for leaves with the rest in the following descending order: Senna > TithoniaEuphatorium > Calliandra > Flemingia. A similar pattern was observed for loss of all nutrients with Calliandra and Flemingia always having lower k values than the rest. Although N immobilization was not observed, immobilization of P and K was observed during the first week of incubation in some species, particularly in stem prunings. Annual biomass of prunings differed significantly between tree species in the following descending order: Calliandra > Senna > Flemingia > Tithonia > Gliricidia > Euphatorium. Calliandra added the greatest amount of nutrients annually to the soil with Euphatorium adding the least. Calliandra prunings provided the annual total K requirement and 49% of the N requirement of mature tea. However, none of the species provided more than 5% of the P requirement. It is concluded that among the tree species tested, Calliandra and Flemingia are the most suitable for contour hedgerows in tea plantations of this agroclimatic region because of their higher soil nutrient enrichment capacity and slower decomposition rates which would minimize leaching losses. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

14.
Heterobasidion parviporum and Heterobasidion annosum are widely distributed root‐rot fungi that infect conifers throughout Europe. Infection of conifer stumps by spores of these pathogens can be controlled by treating fresh stumps with a competing non‐pathogenic fungus, Phlebiopsis gigantea. In this study, growth of three Latvian strains of P. gigantea and the biological control agent ‘Rotstop’ strain was evaluated in stem pieces of Norway spruce, Scots pine, lodgepole pine, Douglas‐fir, Weymouth pine, Siberian larch and Sitka spruce. The growth rates of one H. parviporum and one H. annosum isolate were also measured in the same stem pieces. The growth rate of P. gigantea varied greatly in wood of different conifer species. It was higher in the three pine species, lower in Norway spruce and lowest in Sitka spruce and Siberian larch, and in Douglas‐fir, this fungus did not grow. The largest area of wood occupied by P. gigantea was in lodgepole pine. Growth of Latvian isolates of P. gigantea in the wood of Pinus and Picea species was comparable to that of the Rotstop isolate. Consequently, stump treatment with local P. gigantea isolates should be recommended. However, our results suggest that Douglas‐fir stump treatment against Heterobasidion by P. gigantea may be ineffective and other stump treatment methods should be considered.  相似文献   

15.

The effects of seed weight and seed type on seedling growth of Pinus sylvestris (L.) were studied by seeding individually weighed orchard and stand seed in different mixtures under harsh (direct seeding in field) and optimal (seeding in nursery) conditions. In the nursery experiment an increase in the seed weight from 3 to 7 mg increased the seedling height by 10-27% and total weight by 27-113%, and decreased the height/diameter ratio by 5-6% after 2 yrs. With elimination of seed weight effects, orchard seedlings were 2% taller than stand seedlings in year 2. Without elimination of seed weight effects, orchard seedlings were 7-13% taller. In the field experiment an increase in the seed weight from 3 to 7 mg increased seedling height by 18-65%, stem volume by 81-274% and the number of top-buds by 23-34% in year 5. After elimination of seed weight effects, orchard seedlings were 7-13% taller than stand seedlings and without elimination of seed weight effects 20-21% taller after 5 yrs. Even after elimination of both seed weight and genetic effects orchard seedlings were 3-9% larger than stand seedlings in the field experiment. In conclusion, the influence of seed weight and seed type on growth traits and slenderness is highly significant and the influence seems to be greater in harsh conditions.  相似文献   

16.

This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland.  相似文献   

17.

The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions.  相似文献   

18.
本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。  相似文献   

19.
Baobab leaves form an important part of the local diet in Sahel countries and elsewhere in Africa. Existing leaf nutritional data and agroforestry performance information are based solely on Adansonia digitata L., the baobab of continental Africa. The introduction potential of Adansonia species from the center of diversity in Madagascar and from Australia remains untapped. To assess this potential, the mineral contents and B1 and B2 vitamin levels of dried baobab leaves were determined for five-year old trees of A. digitata, A. gibbosa (A. Cunn.) Guymer ex D. Baum, A. rubrostipa Jum. & H. Perrier (syn. A. fony Baill.), A. perrieri Capuron and A. za Baill. grown in an introduction trial in Mali. Nutritional data were evaluated against survival and vigor to identify promising germplasm. Leaf vitamin and crude protein contents were highest in the Madagascar species, especially A. rubrostipa (B1 88 mg 100 g−1, B2 187 mg 100 g−1, protein 20.7% dry weight). However, the local species far outperformed the introductions in survival, tree height, basal diameter and resistance to termites. We suggest grafting as a way of harnessing the vigor of well-adapted local baobab varieties to the superior nutritional profiles of A. rubrostipa and others. Cross-species grafting tests in Adansonia were successful, thus creating new agroforestry possibilities with different scion/rootstock combinations.  相似文献   

20.
Abstract

Wood in general and wooden studs in particular are often distorted owing to uneven shrinkage during the drying process in the sawmill. Twist is often the most detrimental of all types of distortion, and it is caused by spiral grain in combination with variations in moisture content. For sawmills, the objective is to produce dried, straight boards, and one method of dealing with boards with excessive spiral grain is to sort them out and then dry them in a pretwisted position to obtain straight boards after drying. A model using the finite element (FE) method for the simulation of drying twist distortions was first calibrated against laboratory experiments in which boards were dried with and without restraints and pretwists. After the calibration, the FE results were compared with industrial test results for boards that were dried without restraints or with restraints with zero pretwist, i.e. straight restraints. The FE model used an elastic–ideally plastic material model to obtain permanent deformations. The calibration was to set the yield stresses so that there was a good match between FE results and results from the laboratory experiments. The comparison between the industrial test results and the FE results showed that the FE model is capable of realistic simulations of drying boards with and without restraints and presumably also pretwists.  相似文献   

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