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以贴梗海棠初开花朵为试验材料,研究了TTC、碘-碘化钾2种染色剂测定花粉生活力的效果,离体培养时葡萄糖对花粉萌发的影响和3种不同温度(15、20、25℃)条件下的花粉萌发的变化规律,3种贮藏条件(常温、4℃、-23℃)对花粉贮藏的影响。结果表明:不同染色剂测定的花粉生活力差异显著,TTC染色法(76.26%)显著优于碘-碘化钾染色法(42.46%)。在一定范围内,培养基葡萄糖浓度和萌发温度提高能促进花粉的萌发,超出范围后抑制萌发。试验证明:最佳的葡萄糖浓度为10%,最佳的萌发温度为20℃。贮藏温度对花粉生活力的影响显著,其中最佳的贮藏条件是冷冻干燥,但综合考虑成本、便捷性等因素,实际应用中一般选择4℃。 相似文献
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采用4种不同染色方法及培养基萌发法对5种花色的三色堇花粉生活力进行了测定。结果表明：I-KI染色法、醋酸洋红染色法和TTC染色法不适用于三色堇花粉生活力的测定,而过氧化物酶沉淀法与培养基萌发法是测定三色堇花粉生活力较好的方法;蔗糖和硼酸的浓度影响三色堇花粉的萌发,质量浓度分别为20mg/L、1.5mg/L蔗糖和硼酸对三色堇花粉萌发有很好的促进作用;花粉的生活力与贮藏条件也密切相关。 相似文献
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《辽宁林业科技》2021,(3)
花粉生活力对油松杂交育种至关重要。为筛选油松花粉离体萌发培养基,探讨不同温度贮藏下花粉的生活力,采用正交试验设计,研究不同水平蔗糖、硼酸和pH值对油松花粉离体萌发的影响;测定不同温度贮藏下油松花粉萌发率随时间的动态变化。结果表明:油松新鲜花粉在蔗糖100 g·L~(-1)、硼酸0.10 g·L~(-1)、琼脂8 g·L~(-1)、pH值6.5的培养基上萌发效果最好,萌发率高达93.12%。不同贮藏温度下油松花粉生活力存在明显差异,-80℃贮藏15 d和30 d的花粉萌发率分别为92.93%和91.27%,萌发率下降不显著;-20℃和4℃贮藏15 d,花粉萌发率下降不显著,但贮藏30 d的花粉萌发率与初始萌发率相比均极显著下降,萌发率分别为88.65%和86.84%;常温25℃贮藏15 d、30 d的花粉萌发率分别为90.27%、82.66%,与初始萌发率均达到极显著差异;由此可见,贮藏温度越低,越有利于花粉生活力的保持。此研究可为油松花粉生活力检测提供一种可靠方法,并为花粉贮藏提供参考依据。 相似文献
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《山东林业科技》2015,(1)
以金沙槭(Acer paxii Franch.)和三角枫(Acer buergerianum Miq.)为试验材料,蕾期采粉,并且采用TTC染色法和液体培养基离体萌发法对它们的新鲜花粉,室温条件下(25℃)和4℃冷藏条件下保存的两种花粉进行了生活力测定。结果表明:液体培养基中蔗糖浓度对花粉萌发具有明显影响,三角枫花粉的最佳萌发浓度为10%,而金沙槭花粉的最佳萌发浓度为15%;TTC染色法测定的花粉生活力比液体培养基离体萌发法测定的花粉生活力高;室温条件下(25℃)保存的金沙槭花粉4天后便失去了生活力,三角枫花粉6天后完全失去生活力;4℃低温保存条件下,金沙槭花粉的可贮藏时间较短,约40d后花粉便失去了生活力,而三角枫的花粉贮藏40d后的萌发率为31.37%。 相似文献
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以聚石斛花粉为试材,研究了其花粉生活力的检测方法及贮藏方法。结果表明:(1)检测聚石斛花粉萌发适宜的培养基为100 g·L-1蔗糖+100 mg·L-1H3BO3+80 mg·L-1Ca Cl2,TTC染色法不适用于聚石斛花粉生活力的检测;(2)聚石斛开花第0 8天的花粉生活力高于65%,作为父本,授粉结实率高于85%,显著高于蕾期和衰败期。(3)不同贮藏方法对聚石斛花粉生活力的影响较大,4℃冷藏干燥贮藏80 d后,花粉生活力下降为0,而4℃冷藏湿润贮藏、-20℃冷冻湿润贮藏和干燥贮藏90 d后,花粉生活力分别为1.4%、21.7%、6.4%。经-20℃冷冻湿润贮藏90 d后的花粉经杂交授粉后仍然能够正常结实,其他处理的结实率明显降低。 相似文献
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We present a new approach for selecting seed sources of Eucalyptus to be grown on saline land. We evaluated our method using 22 seed sources, 11 tree-form species and 11 mallee-form species.
We found that the commonly used strategy of selecting seed sources on the basis of average productivity was inappropriate,
as average productivity was poorly correlated with the relationship between productivity and salinity that was actually observed.
Using our new approach, we categorized seed sources as salt-sensitive or salt-tolerant based on the strength of the observed
relationship between salinity and productivity. Seed sources defined as sensitive were associated with relatively large (−24.8
to −64.2%) productivity losses, while the changes in productivity for tolerant seed sources ranged from −17.1 to 10.5%. On
average, reductions in height due to increased salinity were about 30% greater for sensitive seed sources than tolerant ones,
although the effect was smaller for mallee-form and greater for tree-form seed sources. The difference between sensitive and
tolerant seed sources was similar after 1, 2 and 4 years of growth. 相似文献
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王珊子 《绿色中国(A版)》2006,(15):66-68
“民以食为天”,“人命关天”,这两句古话阐明了食品安全无以复加的重要性。实际上,食品安全的重要性还远不止于此,这个问题不仅关系到人的健康和生命,而且关系到经济发展、社会稳定,乃至公民对整个社会和政府的信心。 相似文献
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王珊子 《绿色中国(综合版)》2006,(8):66-68
“民以食为天”，“人命关天”，这两句古话阐明了食品安全无以复加的重要性。实际上，食品安全的重要性还远不止于此，这个问题不仅关系到人的健康和生命，而且关系到经济发展、社会稳定，乃至公民对整个社会和政府的信心。[编者按] 相似文献
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《Journal of Sustainable Forestry》2013,32(3-4):307-316
Concerns for maintaining biodiversity have led to the adoption of ecosystem management as the paradigm for federal land management. This approach will identify desired future conditions as the goal for management, based on ecological objectives for a given landscape. Some management efforts attempt to identify desired future conditions based on existing successional stages as defined by a classification of overstory vegetation types. Such an approach ignores most of the underlying ecological parameters of the landscape, and is inadequate for identifying past disturbance regimes and future successional pathways. An assessment of desired future conditions based on an ecological classification system is essential to overcome these inadequacies. The strategy proposed in this paper uses an appropriate ecological land classification, based on either ecological land types or habitat types, included in a broader hierarchical classification system. It also uses a vegetation map of existing overstory vegetation. These two maps are overlaid to generate polygons of ecological units that can then be used to create an ecosystem diversity matrix. Each polygon (stand) can be evaluated as to its composition and structure relative to its possible placement within the ecosystem diversity matrix through comparisons with historical ranges of variability. The overall ecosystem diversity matrix can then be examined in terms of the distribution of successional stages within each habitat type or ecological land type. The goal should be to maintain at least adequate ecological representation of all successional stages within each habitat type that occurred historically, based on past disturbance regimes. Adequate ecological representation is defined as sufficient size and distribution of inherent ecosystems to maintain viable populations of all endemic species dependent on these ecosystems. This approach can maintain and enhance regional biodiversity, but also maintain flexibility in land management options. 相似文献
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12个桃品种的花芽休眠需冷量和开花需热量 总被引：3，自引：0，他引：3
在浙西南地区研究12个桃品种花芽休眠需冷量和开花需热量生态指标.犹他模型测定结果显示:12个桃品种中,花芽休眠需冷量低的品种为春蜜、超红、中油11号、中油7号、中油4号,约为550～650 cu;其次是丽油5号、仓方早生、燕红、丽油3号、早凤凰,约为650～ 800 cu;需冷量高的品种为新川中岛和赤月,约为900～ 950cu.同一地区不同品种需热量差异较大,同一品种年际间差异相对较小.以生长度天模型试验结果表明:开花需热量低的品种为赤月和丽油3号,大约400 ～ 500 d·℃;其次是新川中岛,大约500～600 d·℃;春蜜、丽油5号、早凤凰、中油4号、中油7号、仓方早生和燕红大约600 ～ 700 d·℃;需热量较高的是中油11号和超红,约为700～800 d·℃.12个桃品种花芽休眠需冷量与开花需热量之间呈乘幂函数曲线显著负相关. 相似文献