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1.
针对青榨槭种子的休眠特性,采取低温层积和赤霉素处理两种催芽方法进行对比试验,分析研究破除青榨槭种子休眠的有效方法.结果表明,青榨槭种子经低温层积14d或500mg/LGA3浸种处理后,发芽指数和发芽率均达到最高,是打破青榨槭种子休眠的最佳方法.  相似文献   

2.
通过采取6种处理措施:赤霉素(GA3)浸种、萘乙酸(NAA)浸种、雪水浸种、变温催芽、机械破壳、层积处理(CK),对处理后的天女木兰种子进行发芽试验。结果看出:该批天女木兰种子的生活力平均值在60%左右;赤霉素处理、机械破壳能缩短发芽时间;采用赤霉素处理和变温处理对解除天女木兰种子深度休眠有较好促进作用,其中赤霉素300mg/L浸泡48h催芽效果最好。  相似文献   

3.
天女木兰种子层积过程中种胚的研究   总被引:3,自引:0,他引:3  
通过对天女木兰种子层积过程中胚的发育、呼吸变化等进行研究,得出休眠的原因及影响其休眠的因素。结论是采用赤霉素浸种变温层积催芽的方法,是解除天女木兰种子休眠的较好途径。  相似文献   

4.
赤霉素浸种与低温层积对桂花种子发芽的影响   总被引:12,自引:0,他引:12  
对桂花种子进行了层积前赤霉素浸种与低温层积相结合的催芽试验。结果表明,桂花种子具有休眠特性,低温层积能够增进桂花种子萌发。层积前用赤霉素浸泡促进桂花种子萌发的作用明显,试验以3000mg/L赤霉素浸泡2天和2000mg/L赤霉素浸泡2天或4天最好,极显著或显著地高于其它处理组合。赤霉素浸泡必须与低温层积相结合,其作用效果才能发挥,试验中以层积60天最好。清水浸泡促进萌发的效果不明显。  相似文献   

5.
以距瓣尾囊草种子为实验材料,采用培养皿发芽法,研究不同浓度赤霉素浸种与层积时间对种子萌发的影响。结果表明:种子具有休眠特性,层积能够促进种子的萌发,时间越长效果越好。层积前用赤霉素浸种处理可显著提高种子发芽率和发芽势。用浓度400 mg·L-1的赤霉素对种子浸泡24 h后层积60 d的萌发效果最好,可使种子的发芽率和发芽势达到83.91%和51.60%。  相似文献   

6.
狭叶冬青种子休眠与萌发的研究   总被引:4,自引:0,他引:4  
采用低温层积、酸蚀处理、赤霉素溶液浸种与低温层积相结合等方法,探讨了狭叶冬青种子休眠的原因和解除休眠的方法.结果表明,狭叶冬青种子经酸蚀处理1.5 h,可使种壳减轻37.6%,降低种壳的机械束缚力,增强了种子的透气性;进一步以赤霉素(GA3)250 mg·kg-1溶液浸种24h和层积处理510 d,发芽率可达21.2%,且发芽整齐,而对照种子发芽率仅为14.4%.  相似文献   

7.
林木种子播前预处理研究   总被引:4,自引:0,他引:4  
对广东省46种林木种子进行播种前预处理研究,结果表明:无休眠特性的种子用45℃温水浸种或随采随播,湿沙储藏,其发芽率均较高;具休眠特性的林木种子,可采用60~100℃热水浸种、化学药剂处理、机械擦伤或层积催芽,可破除种子的休眠,促进种子发芽。  相似文献   

8.
以杜仲种子为研究对象,研究不同处理方法对杜仲种子场圃发芽率的影响。结果表明,温水浸种、层积催芽、赤霉素处理均能提高杜仲种子的场圃发芽率。其中两种温水浸种法场圃发芽率分别为57.6%、58.3%,层积催芽法场圃发芽率为54.6%,200 mg/L赤霉素(GA3)处理法场圃发芽率为54.9%。试验处理间差异不显著,但均显著优于对照。  相似文献   

9.
赤霉素对三种白蜡种子发芽率的影响   总被引:1,自引:0,他引:1  
在不同温度条件下,利用不同浓度的赤霉素对大叶白蜡、绒毛白蜡和水曲柳的种子进行药剂催芽试验,浸种时间均为48 h,分别以经过层积处理60 d的种子作为对照。结果表明,用适宜浓度的赤霉素处理后,在20℃~30℃的变温条件下,可以显著提高大叶白蜡和绒毛白蜡种子的发芽率。催芽大叶白蜡的最佳处理组合为200 mg/L,平均发芽率为71%,催芽绒毛白蜡最佳处理组合为300 mg/L(20℃~30℃),平均发芽率为73%,发芽率均接近各自的对照,催芽效果十分显著。水曲柳具有相对深的休眠特性,单纯赤霉素处理不能有效打破水曲柳种子的休眠。  相似文献   

10.
白蜡类树种是我国重要的用材及绿化树种,8常以种子繁殖为主,实际生产中,在处前常以层积催芽法解除种子休眠,需时约80d。由于需时太长,生产中常因得到种子较晚, 得不延迟播种时节。对白蜡种子进行药剂浸种催芽对比试验。结果表明,50℃始温的温水浸种24h后,用200×10^-6吲哚乙酸(IAA)浸种3h和200×10^-6赤霉素(GA3)浸种24h发芽率分别达到64%和62%,与层积催芽发芽率基本相当,  相似文献   

11.
Mooseer(Allium hirtifolium Boiss.) is bulbous perennial herb widely used in pharmaceutical and food industry in Iran.We studied germination of mooseer seeds in two separate experiments.In the first experiment,we evaluated four treatments:sulfuric acid scarification,sandpaper scarification,cold stratification,and gibberellic acid(GA 3) application.In the second experiment,we evaluated combinations of these treatments.All treatments in the first experiment had no effect on seed germination,suggesting that mooseer seeds have physical and physiological dormancy.In the second experiment,the highest germination percentage(86.6%) was observed after five minutes scarification with sulfuric acid(75% v/v),followed by 60 days of cold stratification.Duration of sulfuric acid scarification(5,10,and 20 min) did not affect germination rates,but increasing duration of cold stratification(from 15 to 60 days),increased germination from 28.3% to 86.6%.Our study showed that mooseer seeds have both physical and physiological dormancy.  相似文献   

12.
通过不同试验材料、GA3应用和变温层积催芽对比试验,对刺楸种子解除休眠进行研究的结果表明:鲜种+GA3+(15℃,30 d;冷冻,90 d;-4~0℃,90 d)处理方法发芽率达到90.2%,出苗率达到81.5%;气干种温水(72 h)+GA3+(13~18℃,90 d;0~4℃,90 d)处理方法发芽率达到76.3%,出苗率达到66.4%。采用鲜种进行变温催芽处理对刺楸种子发芽率、出苗率和1年生苗木质量提高具有显著作用(p<0.01和p<0.05)。研究表明,刺楸种子属于形态-生理休眠类型,采用鲜种+GA3+变温层积催芽的综合处理方法能有效解除刺楸种子休眠。  相似文献   

13.
层积和激素处理对山楂种子生理生化的影响   总被引:1,自引:0,他引:1  
为了研究山楂种子的体眠机理,以野生山楂种子为试材,经1个冬季的层积后,用200 mg/L GA3和ABA处理种子,测定层积与激素处理对种子生理生化指标的影响.结果表明,野生山楂种子层积1个冬天不能解除休眠,其休眠程度较深,经GA3处理后,少量种子萌发,但只长子叶,胚芽和胚根没有萌动.层积后山楂种子的细胞膜透性增加,呼吸加强,总糖、还原糖和游离氨基酸含量降低,可溶性蛋白质含量增加.CAT和SOD活性增加.POD和PAL活性降低.GA3处理使种子CAT、SOD、POD和PAL活性均有所降低,而ABA处理除了使SOD活性降低外,对其余各酶的影响与GA3的作用相反.  相似文献   

14.
Chen SY  Kuo SR  Chien CT 《Tree physiology》2008,28(9):1431-1439
Intact seeds from freshly harvested fruits of Myrica rubra (Sieb et Zucc.) were dormant and required 8 weeks of warm stratification followed by 12 weeks of cold stratification for germination. Exogenous application of gibberellic acid (GA(3)) to intact fresh seeds was effective in breaking dormancy, with > 70% of seeds germinating when treated with 5.2 mM GA(3) and incubated at a day/night temperature of 30/20 degrees C for 20 weeks. Removing the hard endocarp or endocarp plus seed coat of fresh seeds promoted germination, and addition of GA(3) to the embryo accelerated germination. The gibberellins GA(1) and GA(4) were more effective than GA(3) in promoting germination of seeds with the endocarp removed. Endogenous contents of GA(1), GA(3), GA(4), GA(7) and GA(20) were quantified by gas chromatography-mass spectrometry-selected ion monitoring in the endocarps, seed coats and embryos of fresh seeds treated with 5.2 mM GA(3). The content of GA(3) decreased in the endocarp during incubation, whereas GA(1) contents increased in the endocarp and seed coat. A high GA(1) content was detected in the endocarps and embryos of newly germinated seeds. We speculate that GA(3) was converted to GA(1) during incubation and that GA(1) is involved in seed germination. Endogenous abscisic acid (ABA) contents were measured in fresh seeds and in warm and cold stratified seeds. The ABA content in fresh seeds was distributed in the order endocarp > seed coat > embryo, with the content in the endocarp being about 132-fold higher than in the seed coat and embryo. Total ABA content of seeds subjected to warm or cold stratification, or both, was 8.7- to 14.0-fold lower than that of fresh seeds. Low contents of endogenous GA(1), GA(3), GA(7) and GA(20), but elevated contents of GA(4), were found in the seed coats and endocarps of warm plus cold stratified seeds and in the seed coats and embryos of newly germinated seeds. These observations, coupled with the finding that GA stimulated germination of dormant Myrica seeds, provide evidence that endogenous ABA inhibited release of dormancy and that endogenous gibberellins, especially GA(4) or GA(1), or both, are involved in germination.  相似文献   

15.
日本厚朴种子具有休眠习性,常规层积方法催芽时间长且播后发芽率不整齐。研究了昼夜变温、阶段变温以及不同浓度GA处理结合低温层积的方法对日本厚朴种子催芽的效果,实验结果表明:GA处理结合低温层积的方法催芽效果最佳,且随GA浓度升高,催芽效果稍呈上升趋势;昼夜变温处理效果次之,阶段变处理效果最差。  相似文献   

16.
We measured physiological parameters including water uptake, in-vitro embryo germination ratio, and seed coat structure observed by scanning electron microscopy (SEM) to explore the influence of seed coat on the germination of seeds of tallow tree (Sapium sebiferum (Linn) Roxb.). Tallow tree seeds had good water permeability. We found that germination of cabbage seeds was inhibited when cabbage seeds were soaked in extracted solutions from tallow tree seed coat. Seed coat structure at the side of the radicle appeared to be a barrier to seed germination. We tested methods to break tallow tree seed dormancy. Dormancy of tallow tree seeds was overcome by soaking the seeds in 500 mg·L-1 or 1000 mg·L-1 GA3, followed by 100 days of cold stratification.  相似文献   

17.
We used pre-sowing treatments v/z., soaking seeds in concen- trated sulphudc acid (CSA), gibberellic acid (GA), combined treatment of CSA + GA and mechanical scarification to overcome seed dormancy and enhance synchronous germination of Macaranga peltata seeds. We analysed percent seed germination data by logistic regression and con- finned that except in GA treatment, time and acid concentration together were crucial for enhancing germination. The combination treatment of CSA and GA resulted in higher percent germination (up to 74%) than either treatment used separately, but produced defective seedlings (26%). Mechanical scarification of seed coat had the greatest impact in enhanc- ing germination (78%) and reducing imbibition time (6 days) against the control (0%). Germination studies and SEM analysis confirmed that seed germination in M. peltata was inhibited by seed coat dormancy.  相似文献   

18.
种子休眠广泛存在于木本植物, 且其原因复杂多样, 休眠程度深浅不一, 这是目前木本植物种子休眠与萌发的分子生物学研究的主要瓶颈。文中根据Baskin的分类系统, 总结了一些林木种子休眠的归属类型; 对以欧洲水青冈、挪威槭、欧亚槭为阔叶树模式植物的种子休眠, 种子萌发的分子生物学研究进行了综述; 指出相关研究仅限于少量树种的生理休眠类型及与有限领域如激素(ABA、GA及乙烯)信号传导相关的基因及表达和蛋白及其功能, 更多方面如代谢途径、光对种子萌发的调控等研究还有待加强。  相似文献   

19.
Seeds of many woody plant species have one of several types of dormancy. They do not germinate unless specific environmental signals are in place or events occur. This study was conducted to evaluate the effects of scarification treatments on seed dormancy and germination of Acacia nilotica (L.) Willd. ex Del., Prosopis juliflora (Sw.) DC. and Dodonaea viscosa (L.) Jacq. The following treatments were applied: T1, untreated seed (control); T2, sulphuric acid (97%) for 45 min; T3, boiling water for 5 min. The seeds were cultured on a Murashige and Skoog (MS) medium after sterilization. The responses of seeds to treatments were compared with each other and with the control treatment. Germination was observed daily for a 30-day period. Results indicated positive responses to treatments, while impermeable seed coats may be responsible for low germination rates in intact seeds as seen experimentally in the untreated control. The highest germination was obtained for P. juliflora and D. viscosa acid-scarified seeds (80.8%-90.8%) and for scarified seeds of A. nilotica (50.2%) boiled in water. The germination indices, i.e., final germination percentage (FG), mean daily germination (MDG) and germination rate (GR), were significantly affected by treatments and species (p<0.01).  相似文献   

20.
Acer velutinum Boiss is a valuable tree species native to Iran, and its seeds possess physiological dormancy that hampers seedling production in the nursery for large-scale reforestation efforts. The aim of this study was to determine the optimal dormancy breaking treatments for A. velutinum seeds. We conducted a factorial experiment involving six seed lots collected along an elevation gradient from 300 to 1800 m at 300 m interval and four cold-moist stratification periods (0, 4, 8 and 16 weeks) at 4℃ and 70% relative humidity. The result shows that the germination of cold-moist stratified seeds was significantly (p < 0.0001) higher than the control for all seed lots. The highest germination capacity was recorded after 16 weeks of cold-moist stratification for all seed lots (68% 88% depending on the seed lot) except those collected from mid altitude sites (600 and 900 m) that germinated equally well (≥ 75%) after 4and 8-week of clod-moist stratification compared to the other seed lots. The mean germination time was significantly shorter (12 to 19 days, depending on the seed lot) for seeds stratified for 16 weeks than for untreated seeds. It can be concluded that: (1) cold-moist stratification for 16 weeks is the best pre-sowing treatment for breaking dormancy in A. velutinum seeds; and (2) seeds should be collected from mid altitude sites (600 and 900 m) to get more than 80% germination within 15 days, and these seed lots even required shorter cold-moist stratification period(eight weeks) than other seed lots.  相似文献   

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