首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 234 毫秒
1.
为了解新疆昭苏县马匹体表蜱虫和周围游离蜱虫携带马梨形虫情况及其之间流行关系,于2016年4―7月份,分别采集马匹血液、相应马体表携带蜱虫和周围牧场的蜱虫,经PCR检测总携带梨形虫率分别为93.9%(31/33),15.8%(6/38),3.4%(6/175);对所采集的蜱虫进行形态学鉴定,结果分别为草原革蜱、森林革蜱和边缘革蜱;3种革蜱占马体表蜱虫总数的比率和带虫率分别为52.6%(7.9%),44.8%(7.9%),2.6%(0.0%),占牧场游离蜱虫总数的比率和带虫率分别为56.0%(2.3%),43.4%(1.1%),0.6%(0.0%);根据统计学分析,马匹年龄与携带梨形虫及其媒介蜱之间差异显著(P<0.05),马携带蜱虫数量与马源性、蜱源性泰勒虫的感染三者之间均存在显著性差异(P<0.01)。因此,新疆昭苏地区马梨形虫的流行与马匹年龄、带虫媒介蜱虫的数量、种类等因素有关联性。  相似文献   

2.
Vitamin D is essential in calcium and phosphorus regulation, bone physiology, cell proliferation and epithelial integrity. Literature on vitamin D in growing horses is sparse, and the effect of age on vitamin D has not been evaluated in equids in the United States or in tropical countries. The goal of this study was to determine if there was an effect of age on serum 25(OH)D3 concentrations in equids in the US (Ohio/Kentucky) and Thailand (Chiang Rai and Kanchanaburi) during the same time of the year. Blood samples were collected from healthy ponies (n = 21) and Thoroughbred foals (n = 13), yearlings (n = 10), and horses (n = 20) in Thailand and from Thoroughbred foals (n = 10) and horses (n = 17) in the US. Serum concentrations of 25(OH)D3, calcium and phosphorus were measured.In both countries, serum 25(OH)D3 concentrations were lower in foals than in yearlings and adult horses. Serum 25(OH)D3 concentrations were higher in horses than in ponies in Thailand, but were not different between horses from either country. Calcium concentrations were not different between groups or location. In both countries, phosphorus concentrations were higher in foals than in older groups; however, were not different between ponies and horses. This study shows that independent of geography there are age-related differences in 25(OH)D3 concentrations in horses and further confirms that 25(OH)D3 concentrations are lower in horses compared to other species. The information will serve as the basis for future clinical studies and to help understand better the pathophysiology of equine disorders associated with calcium and phosphorus dysregulation.  相似文献   

3.
应用５因素２次回归几乎正交旋转组合设计，运用主成分分析方法对４３２只ＡｒｂｏｒＡｃｒｅｓ公雏（３６组，１２只／组）的日粮钙、磷、锰、锌、ＶＤ３５因素与９项检测指标之间的关系进行研究。相关性分析和主成分分析结果表明：日粮钙水平与肉仔鸡１４日龄体重、日粮钙和锌水平与肝脏锌沉积、日粮磷水平与ＡＫＰ活性、胫骨锌沉积均呈显著负相关；日粮锌水平与肉仔鸡体重、胫骨锌沉积呈强正相关。分析累计方差贡献率８６％以上的１１个主成分表达式结果：日粮钙水平阻碍肉仔鸡早期生长，使组织锌沉积降低；日粮钙与磷、锰与锌、ＶＤ３与磷之间的互作在不同程度上影响肉仔鸡组织、血液和骨骼锌沉积。衡量日粮钙供给状况的是第１、９、１０主成分因子；衡量日粮钙、磷供给状况的是第３、８、１１主成分因子；衡量日粮锰与钙供给状况的是第４主成分因子；衡量日粮锌与钙供给状况的是第５主成分因子。  相似文献   

4.
从发病猪的关节液中分离细菌,镜检后确定为链球菌.将该菌株培养后进行分群鉴定、生化试验及耐药性试验.结果表明,分离的细菌为链球菌D群.该菌株对大部分药敏试剂都不敏感,耐药性偏高.  相似文献   

5.
  相似文献   

6.
  相似文献   

7.
ABSTRACT

Aim: To determine if vitamin D3 treatment reduced the incidence of vaginal prolapse in pregnant sheep on a North Canterbury sheep breeding property.

Methods: Pregnant ewes from a single farm were allocated to three treatment groups in May 2018. At this time, the first group (EarlyVitADE; n?=?512) received an I/M 1?mL dose of 500,000?IU/mL vitamin D3, 60,000?IU/mL vitamin A, and 25?mg/mL vitamin E. This was repeated in July 2018, when the second group (LateVitADE; n?=?695) also received the same treatment. The third group (n?=?737) were untreated controls. All cases of vaginal prolapse on the property were recorded from pregnancy diagnosis in June 2018 until ewes were set-stocked in August 2018. The planned start of lambing was 10 August 2018.

Results: During the period of observation, vaginal prolapses were recorded in 3/699 (0.4%) 2-year-old ewes, and the odds of vaginal prolapse were not associated with treatment group in these ewes (p?>?0.3). Amongst ewes aged ≥3 years, during the same period, there were 6/333 (1.8%), 6/443 (1.4%) and 25/469 (5.3%) cases in the EarlyVitADE, LateVitADE and control groups, respectively. Compared to control ewes, the odds of vaginal prolapse were reduced in both the EarlyVitADE (OR?=?0.37; 95% CI?=?0.15–0.92) and LateVitADE (OR?=?0.25; 95% CI?=?0.10–0.62) treatment groups.

Conclusions and clinical relevance: In this preliminary study, administration of injectable vitamins A, D3, and E to pregnant ewes reduced the incidence of vaginal prolapse during the period from pregnancy diagnosis to set-stocking on one North Canterbury hill-country farm. Due to the restricted data collection period, this investigation should be replicated to better quantify the repeatability of the observed treatment effect over the complete lambing period.  相似文献   

8.
A pregnant 2-year-old alpaca was presented for evaluation of progressive weight loss, decreased appetite and lethargy that developed in winter. Haematologic and serum biochemical analyses revealed marked anaemia, leukopenia, severe hypophosphataemia and mild hypocalcaemia. Evaluation of bone marrow core biopsies and aspirates revealed an increased proportion of immature haematopoietic cells, without sufficient numbers of blast cells to be termed an acute myeloid leukaemia (AML). 1 The haematological and bone marrow findings were suggestive of myelodysplastic syndrome (MDS). The anaemia, leukopenia, lethargy and weight loss remained refractory to medical therapy and the alpaca was euthanased on humane grounds.  相似文献   

9.
本试验旨在研究当日粮钙磷比一定时,维生素D和钙水平对冬毛期水貂生长性能、营养物质消化率及氮、钙、磷代谢的影响。随机选取（135±5）日龄、健康雄性水貂117只,分成9组,每组13个重复,每个重复1只。采用3×3双因素随机试验设计,设3个维生素D水平,分别为2 300、4 300、6 300 IU/kg;设3个钙水平,分别为2.0%、2.4%、2.8%,钙磷比固定为1.7∶1,配制9组试验日粮,分别为：Ⅰ组（2 300 IU/kg维生素D＋2.0%钙）、Ⅱ组（4 300 IU/kg维生素D＋2.0%钙）、Ⅲ组（6 300 IU/kg维生素D＋2.0%钙）、Ⅳ组（2 300 IU/kg维生素D＋2.4%钙）、Ⅴ组（4 300 IU/kg维生素D＋2.4%钙）、Ⅵ组（6 300 IU/kg维生素D＋2.4%钙）、Ⅶ组（2 300 IU/kg维生素D＋2.8%钙）、Ⅷ组（4 300 IU/kg维生素D＋2.8%钙）、Ⅸ组（6 300 IU/kg维生素D＋2.8%钙）。预试期13 d,正试期60 d。结果表明：①各组水貂料重比差异极显著（P<0.01）,Ⅱ组最低。钙水平对料重比有显著影响（P<0.05）,维生素D水平对料重比有极显著影响（P<0.01）。②钙水平可极显著影响粗脂肪消化率（P<0.01）,其中2.8%水平极显著低于2.0%和2.4%水平（P<0.01）。③日粮维生素D和钙水平对水貂氮代谢指标均无显著影响（P>0.05）。④各组水貂粪钙、粪磷、钙消化率、磷消化率均差异极显著（P<0.01）,其中Ⅱ组钙消化率、磷消化率最高,Ⅲ组次之。钙水平可极显著影响粪钙、粪磷、钙消化率、磷消化率（P<0.01）,2.0%水平可极显著降低粪钙、粪磷含量（P<0.01）,极显著提高钙消化率及磷消化率（P<0.01）。维生素D水平对钙消化率影响显著（P<0.05）,对粪磷、磷消化率影响极显著（P<0.01）,2 300~4 300 IU/kg维生素D水平有利于提高钙消化率、磷消化率,降低粪磷含量。综合考虑各项指标,在本试验条件下,日粮中钙磷比为1.7∶1,维生素D水平为2 300~4 300 IU/kg、钙水平为2.0%时,冬毛期水貂料重比及钙、磷排放量较低,而粗脂肪、钙、磷消化率较高。  相似文献   

10.
1. The aim of the present study was to examine the effects of improving vitamin D status in broiler diets by supplementary 25-hydroxycholecalciferol (25OHD3), alone or in combination with calcium (Ca) and available phosphorus (aP), on live performance, sternum mineralisation and breast meat quality in broilers.

2. A total of 936 1-d-old Ross 308 broilers were used in the study. After gender determination at the hatchery, chicks from each sex were randomly distributed into three dietary treatments. The following dietary treatments were used in the experiment from hatch to 38 d: (1) A control diet formulated to meet all of the nutrient requirements of broiler chicks according to the management guide; (2) The control diet supplemented with 18.7–15.0 µg/kg of 25OHD3; and (3) The control diet supplemented with 18.7–15.0 µg/kg of 25OHD3 plus Ca + aP.

3. Improvement in vitamin D status by 25OHD3 supplementation, alone or in combination with Ca and aP, had no effect on body weight and feed conversion ratio of broilers.

4. The serum 25OHD3 concentration significantly increased with 25OHD3 and 25OHD3 plus Ca + aP supplementation (P < 0.05), whereas the ionised Ca and Mg concentrations remained unchanged.

5. Sternum absolute weight, ash content and the concentrations of Ca and P significantly increased (P < 0.01) with supplementation of 25OHD3, alone or in combination with Ca + aP.

6. Supplemental 25OHD3, alone or in combination with Ca + aP, slightly increased pH24 (P = 0.05) and decreased (P < 0.01) squeezable water loss in breast meat, whereas it had no significant effect on lightness, yellowness and sarcoplasmic protein solubility.

7. In conclusion, the results suggested that enhancing vitamin D status by 25OHD3 supplementation alone or in combination with Ca + aP may improve sternum structure and mineral accretion. Furthermore, supplemental 25OHD3, even in a nutritionally complete diet, may offer an effective way to improve protein solubility in female broilers.  相似文献   


11.
柞蚕抗菌肽D杀菌机理研究   总被引：10，自引：2，他引：8  
李文楚  戴祝英 《蚕业科学》1991,17(3):163-168
本文研究柞蚕抗菌肽D对水稻白叶枯病细菌及番茄、烟奇、马铃薯青枯病细菌的杀菌作用及其机理。在37°C,pH6.8条件下,最低有效剂量2ng/1×10~5活菌。通过电镜观察,抗菌肽D对大肠杆菌及白叶枯病细菌的作用,首先使其外壁层及细胞质膜损伤,尤以菌体两端更为明显。此后形成喇叭状缺口,内容物呈囊状挤出胞外而致死。抗菌肽作用的菌体表面形成微管通道,钾离子大量渗出,但菌体外形在短时间内不致崩坏。  相似文献   

12.
A study was conducted to determine the circadian rhythms and trends of vitamin D metabolites including 25‐hydroxyvitamin D3, 25‐hydroxyvitamin D2, 1,25‐dihydroxyvitamin D and parathyroid hormone, in addition to serum calcium, phosphorus and magnesium concentrations in horses over 48 h on the shortest and longest days of the year in 2013. Five healthy adult horses (Equus caballus) were on a constant pasture feeding regimen, and blood samples were collected from each horse every 3 h over a 48‐h period, starting at 07:00 PM on day one and finishing at 07:00 PM on day three, for the measurement of calciotropic hormones and electrolytes. There was a significant difference between the serum concentration of calciotropic hormones, iCa, tCa, P and tMg between the shortest (winter) and longest (summer) days of the year in horses. Serum concentration of 25OHD3 was very low and mostly undetectable. Serum iCa, 1,25(OH)2D and PTH concentrations clearly showed a circadian rhythm on the longest days of the year and serum tCa, P and tMg concentrations showed a diurnal pattern on the longest days (summer) of the year. None of the analytes showed any circadian rhythm on the shortest days (winter) of the year. The result of this study could have significant relevance to equine athletes travelling to international equestrian competitions and facing a huge time and seasonal differences that might affect their ability to adjust their circadian rhythms to new time zones.  相似文献   

13.
  相似文献   

14.
Antioxidant enzymes, such as catalase, superoxide dismutases (SOD), MnSOD and Cu/ZnSOD, protect cells by scavenging reactive oxygen species (ROS). Numerous studies have reported the anti‐cancer effects of 1,25‐dihydroxyvitamin D3 (calcitriol) and its related analogues, seocalcitol and analogue V. In this study, canine bladder transitional cell carcinoma (cbTCC) cells were used to determine effects of calcitriol and its related analogues on antioxidant enzyme gene expression, protein expression and activity. Catalase mRNA was increased in response to calcitriol (10?7 M), and seocalcitol (10?7 and 10?9 M). MnSOD mRNA was decreased in response to calcitriol at 10?7 M. Catalase was significantly increased in response to calcitriol (10?7 and 10?9 M), and seocalcitol (10?9 M). Catalase enzymatic activity increased in response to calcitriol, seocalcitol and analogue V (10?9 M). In addition, global gene expression analysis identified the involvement of mitogen‐activated protein kinase (MAPK) signalling in cbTCC's response to calcitriol and seocalcitol treatment.  相似文献   

15.
  相似文献   

16.
Vitamin D is an important hormone in vertebrates, and most animals acquire this hormone through their diet and/or exposure to ultraviolet B (UVB) radiation. To date, no study has determined how chinchillas (Chinchilla laniger) acquire vitamin D. The objective of this study was to determine whether exposure to UVB radiation had an effect on serum 25-hydroxyvitamin D levels in captive chinchillas. Overall, 10 juvenile chinchillas were used for this scientific investigation. Baseline blood samples were collected from the animals while under isoflurane anesthesia to determine their serum 25-hydroxyvitamin D concentrations. The chinchillas were then randomly assigned to 2 treatment groups: Group A, 12 hours of UVB exposure per day and Group B, no UVB exposure. At the end of the study (Day 16), a second blood sample was collected from each animal while again under isoflurane anesthesia to measure serum 25-hydroxyvitamin D concentrations. Mean ± standard deviation serum 25-hydroxyvitamin D concentrations differed significantly (P = 0.048) between juvenile chinchillas provided supplemental UVB radiation (189.0 ± 102.7 nmol/L) and those not provided supplemental UVB radiation (87.8 ± 34.4 nmol/L). This study found that exposing juvenile chinchillas to UVB radiation significantly increased their circulating serum 25-hydroxyvitamin D levels. Because vitamin D is an essential hormone in vertebrates, these findings suggest that the provision of UVB radiation to captive chinchillas may be important. Further research to elucidate the importance of 25-hydroxyvitamin D and UVB radiation in captive chinchillas is required.  相似文献   

17.
经对宁夏4个地区1085只家兔和35只野兔的调查,发现有3种双腔属（Dicrocoelium）吸虫:矛形双腔吸虫（D.lanceatum）、东方双腔吸虫（D.orientalis）和中华双腔吸虫（D.chinensis）。其中后2种为宿主新纪录。它们在家兔中的感染率依次为4.2％、1.8％、0.4％,在野兔中的感染率依次为14.3％、5.7％、5.7％。中华双腔吸虫呈纺锤形,体长5.702～6.934mm,体宽1.091～1.549mm,长宽比4.9:1,睾丸并列,阴茎囊椭圆形并达腹吸盘前缘,卵黄腺起自于睾丸之后、卵巢之前水平处;其余2虫种的形态与记载的基本一致。  相似文献   

18.
During the winter time in Finland, sunlight is inadequate for vitamin D synthesis. Many pet rabbits live as house rabbits with limited outdoor access even during summer and may therefore be dependent on dietary sources of vitamin D. The aims of this study were to report the serum 25‐hydroxyvitamin D concentrations in Finnish pet rabbits and to identify factors that influence vitamin D status. Serum 25‐hydroxyvitamin D concentrations from 140 pet rabbits were determined using a vitamin D enzyme immunoassay (EIA) kit. Eleven rabbits were excluded from the statistical analysis because of unclear dietary data. The remaining 129 rabbits were divided into groups depending on outdoor access during summer (no access n = 26, periodic n = 57, regular n = 46) as well as daily diet: little or no hay and commercial rabbit food ≤1/2 dl (n = 12); a lot of hay and no commercial food daily (n = 23); a lot of hay and commercial food <1 dl (n = 59); a lot of hay and commercial food ≥1 dl (n = 35). The range of serum 25‐hydroxyvitamin D concentration was from 4.5 to 67.5 ng/ml with a mean of 26.1 ng/ml. Statistical general linear model adjusted for weight, age and season indicated that diet was associated with vitamin D concentrations (p = 0.001), but outdoor access during summer was not (p = 0.41). Mean 25‐hydroxyvitamin D concentration was significantly higher in the rabbits receiving a lot of hay and commercial food ≥1 dl (33.9 ± 13.2 ng/ml) than in rabbits in other diet groups (24.0 ± 8.5 ng/ml, 21.7 ± 8.1 ng/ml, and 22.2 ± 18.0 ng/ml, respectively). This investigation showed wide variation in 25‐hydroxyvitamin D concentrations among Finnish pet rabbits. Diet remains a main source since outdoor access seems to be too limited to provide adequate vitamin D synthesis for most of them, and the use of vitamin D supplements is rare.  相似文献   

19.
水貂多巴胺受体D2基因的克隆测序及外显子区多态性检测   总被引：1，自引：0，他引：1  
以多巴胺受体D2基因(DRD2)作为候选基因,分析该基因对水貂自咬行为的影响。本试验采用聚合酶链式反应方法,首次从美洲黑貂脚部肌肉组织扩增出多巴胺受体D2基因的部分序列片段,并克隆测序,将该序列提交到Gene Bank上,已得到序列号为EU085471。通过序列比较,在第四外显子区域未发现碱基突变,在第5外显子区域发现在41位点处发生了(T→C)转换,采用单链构象多态性(PCR-SSCP)方法进行了多态性检测,结果表明:在不同个体中未检测到基因多态性。  相似文献   

20.
为构建和表达抗RT单链抗体(ScFv)蛋白,用RT-PCR方法从能分泌特异性抗RT单克隆抗体(McAb)的杂交瘤细胞中分离纯化抗体VH和VL基因。用重叠延伸PCR方法将VH和VL拼接在一起,构建抗RT-ScFv基因。将ScFv基因连接到pMAL-p2X表达载体,转化TB1表达菌。阳性克隆用IPTG诱导18h,Western blotting鉴定重组蛋白。结果表明,试验成功扩增出了ScFv基因,长度约为750bp。通过DNA序列测定和分析,构建出VL-(Gly4Ser)3-VH。其VH全长363bp,可编码121个氨基酸,VL全长324bp,可编码108个氨基酸。SDS-PAGE和Western blotting分析结果表明,抗RT-ScFv在TB1表达菌中获得高效表达,pMAL-p2X表达的ScFv加上同时融合表达的MBP标签分子质量约为75ku。本试验成功构建了pMAL-RT-ScFv表达载体,并获得了高效表达。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号