首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 31 毫秒
1.
ABSTRACT

Legumes, including hairy vetch (Vicia villosa Roth), are widely used as green manures. They fix nitrogen (N) and provide the N to other crops when they decompose, and thus are considered alternatives for chemical N fertilizers. However, N-rich plant residues, including hairy vetch, are also sources of soil nitrous oxide (N2O) emissions, a greenhouse gas. On one hand, rice (Oryza sativa L. ssp. japonica) husk biochar is widely used as a soil conditioner in Japan and has been reported as a tool to mitigate soil N2O emissions. We conducted a soil core incubation experiment (1.5 months) to compare the N2O emissions during the decomposition of surface-applied hairy vetch (0.8 kg dried hairy vetch m?2 soil) under semi-saturated soil moisture conditions (~100% water-filled pore space (WFPS)), using two soil types, namely Andosol and Fluvisol. Throughout the incubation period, the use of biochar suppressed soil NH4+-N concentrations in Andosol, whereas the effect of biochar on NH4+-N was not clear in Fluvisol. Biochar increased the nitrate (NO3?-N) levels both in Andosol and Fluvisol, suggesting a negative influence on denitrification and/or a positive influence on nitrification. Biochar application did not influence the cumulative N2O emissions. Our study suggests that rice husk biochar is not a good option to mitigate N2O emissions during the decomposition of surface-applied hairy vetch, although this study was performed under laboratory conditions without plants. However, the trends of the inorganic-N concentration changes followed by the addition of hairy vetch and biochar were markedly different between the two soil types. Thus, factors behind the differences need to be further studied.  相似文献   

2.
The reliability of the most probable number (MPN) method for estimating bradyrhizobial numbers was evaluated by comparison with the plate count procedure. MPN estimates increased with time of nodulation scoring after seedling inoculation through 6 weeks of incubation. Ratios of MPN to plate counts increased as the bradyrhizobial cell suspension concentration increased. The MPN method could not detect Bradyrhizobium japonicum numbers at concentrations of 103 colony forming units (CFU) ml-1 and below. A proposal for re-evaluating MPN estimates is discussed.  相似文献   

3.
Volcanic ash soils along the western edge of the Omiya tableland, Japan, are covered with thick anthropogenic soil horizons. The formation of anthropogenic soil horizons occurs because of the soil dressing practice known as “Dorotsuke,” where alluvial soil materials are deposited on fields and mixed with volcanic ash topsoil by tillage over the years. To clarify the chronology of this anthropedogenesis, carbon-14 (14C) age profiles were estimated using humic acid fractions from three pedons: an anthropogenic soil, an undressed Andosol, and a Fluvisol. Soil charcoal fragments were also dated to estimate maximum burial age. Charcoal fragments displayed vertically random age distributions, indicating that the fragments may have had multiple origins. However, the age of charcoal in the lower part of the anthropogenic soil horizons indicated that the initiation of anthropedogenesis occurred later than the late 13th century. The 14C age profile of humic acid in the Andosol exhibited little variation in age with depth in the subsoil. The 14C age profile of humic acid in the Fluvisol suggested that the humic acid fraction included allochthonous old carbon (C), although the soil itself had been formed from recent sediments. The 14C age profile of humic acid in the anthropogenic soil showed features of its two component soils. The 14C ages in the volcanic ash subsoil matched with those in the Andosol, whereas the ages increased in the anthropogenic soil horizons because of supplementation with old C from alluvial soil materials. However, the peak 14C ages occurred in the lower part of the anthropogenic horizons, whereas the middle part on the peak position displayed a gradual age-depth gradient. This feature was interpreted as a sign of 14C activity equilibrium throughout anthropedogenesis. On the basis of this postulated 14C activity equilibrium, the linear age-depth gradient at the peak position was derived from differences in burial time, and burial ages were calculated by estimating steady-state 14C. The calculated ages were lower than the charcoal ages. These age estimates suggest that anthropedogenesis was initiated in the Middle Ages and reached an intermediate stage before or during the first half of the Edo period.  相似文献   

4.
Summary Potential denitrifying activity and population dynamics of Azospirillum lipoferum (137C) and Bradyrhizobium japonicum (G2sp) inoculated into a -sterilized soil were studied for a period of 3 weeks. The denitrifying enzyme potential of soil inoculated independently with each bacterial species was strongly stimulated by the presence of a plant (Zea mays L.). Simultaneous inoculation of both bacteria also produced a higher denitrifying enzyme potential than simple inoculation. Even with double inoculation, the presence of a plant did not modify the evolution of the activity. The response of the population dynamics to these treatments followed a different pattern. The population dynamics of A. lipoferum was not affected by the presence of the plant or by the presence of B. japonicum. In contrast, the presence of both a plant and of A. lipoferum seemed to promote the growth of B. japonicum.  相似文献   

5.
Volcanic ash soil, which is widely distributed in Japan, contains a large amount of well-structured soil aggregates. By using these aggregates as carrier materials, we prepared (brady)rhizobial inoculants for red kidney bean (Phaseolus vulgaris) and soybean (Glycine max). Autoclaved soil aggregates were inoculated with Rhizobium tropici CIATS99R or Bradyrhizobium japonicum USDA110R, incubated for 15 or 21 d at 30°C, slowly air-dried at 20°C to prepare the aggregate-based inoculants, and stored at various temperatures. The populations of CIATS99R and USDA110R in the aggregate-based inoculants were maintained during several months of storage at 20°C. When the aggregate-based inoculants were mixed with soil, CIATS99R and USDA110R cells showed a remarkably improved survival in soils compared with those mixed with soil without carrier material. The effect of the aggregate-based inoculants on the growth of red kidney bean and soybean was examined in pot experiments. By placing a small amount of the inoculant just beneath the seeds at the time of sowing, plant growth was significantly enhanced compared with the use of traditional peat-based inoculant. In addition, nodule formation on the upper part of soybean roots and nodule occupancy by the inoculated strain were remarkably enhanced by the aggregate-based inoculant. It is suggested that soil aggregates might be suitable carrier materials for preparing cheap and effective (brady)rhizobial inoculants.  相似文献   

6.
The effects of inoculation with Bradyrhizobium japonicum and Azospirillum brasilense strains on the growth of soybean were evaluated with regard to the estimation of N2 fixation using the 15N isotope dilution technique. Inoculation, in general, increased the dry mass of soybean as well as nitrogen content. Dual inoculation with a mixture of B. japonicum and A. brasilense strains was superior over single inoculation with B. japonicum. Nitrogen fixed (Ndfa) varied according to inoculant and soil conditions. Percentages of nitrogen derived from air (% Ndfa) using a non-nodulating isoline were 72% and 76% for B. japonicum and B. japonicum plus A. brasilense, respectively, in non-sterile soil. A similar but higher trend was recorded in sterilized soil, in which the percentages of N2 fixed were 81% and 86% for single and dual inoculation, respectively. The correlation coefficient between N2 fixed and N uptake (r=0.94) and dry mass (r=0.89) was significant. Application of special bacterial inoculants in agricultural systems of Egypt seems to be a promising technology and could be used for improving soybean growth as well as soil fertility, thus minimizing environmental pollution. Received: 10 January 1996  相似文献   

7.
Summary Soil solarization greatly reduced the native chickpea Rhizobium population. With inoculation, it was possible to increase the population of the Rhizobium in solarized plots. In the 1st year, 47% nodulation was obtained with chickpea inoculant strain IC 59 when introduced with a cereal crop 2 weeks after the soil solarization and having a native Rhizobium count of <10 g-1 soil, and only 13% when introduced 16 weeks after solarization at the time the chickpeas were sown, with 2.0×102 native rhizobia g-1 soil. In the non-solarized plots inoculated with 5.6×103 native rhizobia g-1 soil, only 6% nodulation was obtained with the inoculant. In the succeeding year, non-inoculated chickpea was grown on the same plots without any solarization or Rhizobium inoculation. The treatment that showed good establishment of the inoculant strain in year 1 formed 68% inoculant nodules. Other treatments indicated a further reduction in inoculant success, from 1%–13% to 1%–9%. Soil solarization thus allowed an inoculant strain to successfully displace the high native population in the field and can serve as a research tool to compare strains in the field, irrespective of competitive ability. In year 1, Rhizobium inoculation of chickpea gave increased nodulation and increased plant growth 20 and 51 days after sowing, and increased dry matter, grain yield, and grain protein yield at maturity. These beneficial effects of inoculation on plant growth and yield were not measured in the 2nd year.Submitted as Journal Article No. JA 945 by the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh 502 324, India  相似文献   

8.
Summary In Japan some paddy fields are used for upland crops for several years and then returned to paddy fields (paddy-upland rotation). Soybeans (Glycine max L.) are an important summer crop. The ability to denitrify and some characteristics of denitrification by isolated strains of Bradyrhizobium japonicum were investigated to clarify the frequency of denitrifiers in indigenous populations of B. japonicum in fileds under paddyupland rotation. Eight field plots with different cropping systems at two sites were used. The fields consisted of a Gray Lowland Soil, and either soybeans or paddy rice (Oryza sativa L.) was grown as a summer crop, with barley (Hordeum distichum L.) or wheat (Triticum aestivum L.) as a winter crop. All B. japonicum strains present in the plots were able to denitrify. Isolated strains fell into two main groups (groups I and II) according to the rate of denitrification. Strains of group I evolved N2O with C2H2 at a rate comparable to that of Alcaligenes denitrificans IAM 12370, whereas the denitrification activity of group II strains was 100 times lower than that of group I strains. Both group I and II strains occurred in each plot. Amounts of N2O produced by indigenous strains with and without C2H2 suggested that strains of group I and II evolved N2 or N2O, respectively, as the end product of denitrification. One strain (S 107) that was isolated had the highest denitrifying ability with an end product of N2O. These results indicate that indigenous bradyrhizobia may partly contribute to denitrification of field soil under a paddy-upland rotation.  相似文献   

9.
Summary Studies were performed to assess the influence of percolating water and an advancing wetting front on the transport of Bradyrhizobium japonicum in sand and silt loam soils, and to assess the influence of clay content on water-facilitated dispersal of these bacteria in a sand amended with various amounts of kaolin. The data obtained showed that movement of B. japonicum in soil was dependent upon water movement and that both percolating water and an advancing wetting front readily transported bacteria in coarse-textured soils. Percolation with the equivalent of 10 cm of rainfall dispersed B. japonicum throughout 40-cm columns containing sand and silt loam soils. Percolation with 5 cm of water was sufficient to disperse B. japonicum throughout 20-cm columns of these soils but did not transport these bacteria below the surface 4 cm of a sand amended with 12% kaolin. Our finding that cells of B. japonicum are readily transported by an advancing wetting front indicates that non-saturated flow of soil water contributes to dispersal of inoculum in soils.  相似文献   

10.
Bradyrhizobium japonicum strain CB 1809 was recently chosen to replace strain WB 1 in commercial soybean [Glycine max (L.) Merr.] inoculants in South Africa, the selection criterion being N2-fixing effectiveness. Nodulation competitiveness is an additional characteristic required of inoculants and was determined for CB 1809 and WB 1 as well as two other strains, USDA 110 and a Brazilian strain 965, using the gusA marker gene to identify strains. Initial experiments with plants grown in sterile sand showed that the competitive index of strain WB 1 was less than that of the other strains. Further comparisons used plants grown in five soils containing established populations of B. japonicum. When strains were applied in peat inoculum to seed at a rate of 1,000 cells per seed in a soil containing 300 rhizobia g–1, significant differences in nodule occupancy were detected and strains ranked in the order 965>CB 1809>USDA 110>WB 1. The remaining four soils each contained about 106 rhizobia g–1 and 5×106 cells were applied per seed. Nodule occupancy by inoculant strains ranged from 22% to 81% between soils. In this experiment, WB 1 was consistently the poorest performer and its competitiveness was significantly less than CB 1809. The competition results supported the recent decision to replace WB 1 with CB 1809 in commercial inoculants. Although WB 1 had been used in inoculants over a period of 19 years, this strain was detected in only one soil, where it comprised 8% of isolates. In contrast, a substantial proportion (32–78%) of isolates from the soils corresponded serologically to a former inoculant strain WB 66, which had been discontinued in 1966. This illustrates the difficulty of replacing a resident population with an introduced strain. The effect of naturalized populations on the establishment of CB 1809 in South African soils will need monitoring Received: 23 November 1999  相似文献   

11.
Summary The ability of Bradyrhizobium strains to survive saprophytically in soil was studied by means of fluorescent antibodies (FA). It was found that the recovery rate may be considered a constant value although the limit of detection by the FA technique is approximately 103 bacteria g–1 soil. By studying the survival kinetics of B. japonicum strains introduced into soils, we observed that whichever soil-strain combination was tested in a given soil during the incubation all the different populations of a strain reached the same survival balance level, generally about 103–104 Bradyrhizobium g–1 soil. When we reintroduced strains into a soil containing rhizobia of the same specificity, the new inhabitants reached the same equilibrium level as that of the initial population. The balance threshold level does not appear to be a very sensitive way to classify, strains on their saprophytic ability. We suggest that survival kinetics should be characterized by the rate at which the population reaches equilibrium.  相似文献   

12.
本文研究了大豆根瘸菌PRC005的接菌量对大豆生长的影响.田间试验结果表明:接菌量在播种后40天和60天没有显著增加根瘤数、根瘤干重、地上部植株干重和植株含氮量.施氮肥处理和较高接菌量处理之间的大豆种子产量差异不显著,与不接菌对照处理相比,施氮肥和较高接菌量两个处理的种子产量显著增加.施氮肥处理没有使植株含氮量增加,并且还妨碍了大豆的结瘸作用.室内盆栽试验结果表明:只有当接菌量高于土著菌数1200倍时,才能显著地提高大豆的结瘤数和植株干重.  相似文献   

13.
《Applied soil ecology》2007,35(1):57-67
Soils of many potential soybean fields in Africa are characterized by low levels of biological nitrogen fixation (BNF) activities and often cannot support high soybean yields without addition of inorganic N fertilizers or external application of soybean rhizobia. The most probable number (MPN) technique was used to determine the bradyrhizobial populations that nodulate TGx soybean genotypes (a cross between nonpromiscuous North American soybean genotypes and promiscuous Asian soybean genotypes), cowpea or North American soybean cv. Clark IV, in soils from 65 sites in 9 African countries. The symbiotic effectiveness of isolates from these soils was compared to that of Bradyrhizobium japonicum strain USDA110. The bradyrhizobial population sizes ranged from 0 to 104 cells g−1 soil. Bradyrhizobium sp. (TGx) populations were detected in 72% and B. japonicum (Clark) in 37% of the soil samples. Bradyrhizobium sp. (TGx) populations were generally low, and significantly less than that of the cowpea bradyrhizobial populations in 57% of the samples. Population sizes of less than 10 cells g−1 soil were common as these were detected in at least 43% of the soil samples. B. japonicum (Clark) occurred in higher population densities in research sites compared to farmers’ fields. Bradyrhizobium sp. (TGx) populations were highly correlated with biotic but not abiotic factors. The frequent incidence of low Bradyrhizobium sp. (TGx) populations is unlikely to support optimum BNF enough for high soybean yields while the presence of B. japonicum (Clark) in research fields has the potential to compromise the selection pressure anticipated from the indigenous Bradyrhizobium spp. (Vigna) populations. Bradyrhizobium isolates could be placed in four symbiotic phenotype groups based on their effectiveness on a TGx soybean genotype and the North American cultivar Clark IV. Symbiotic phenotype group II isolates were as effective as B. japonicum strain USDA110 on both soybean genotypes while isolates of group IV were effective on the TGx soybean genotype but not on the Clark IV. The group IV isolates represent a unique subgroup of indigenous bradyrhizobia that can sustain high soybean yields when available in sufficient population densities.  相似文献   

14.
Summary A field experiment was condutced in a clay loam soil to study the performance of three Bradyrhizobium japonicum strains; USDA 110, USDA 138 and TAL 379, in relation to their N2-fixing potential and competitiveness on two soybean cultivars (Clark and Calland). Inoculation of soybean cultivars with these strains, either singly or in combination, induced significant increases in plant dry weight, N2 fixation and seed yields. However, no significant differences were found between the rhizobial strains and/or their mixtures in N2 fixation and increased seed yield for both cultivars. The two soybean cultivars gave similar responses to inoculation. No significant differences in seed yield were observed between Clark and Calland cultivars. The interaction between inoculant strain and soybean cultivar was not significant. The competition between strains for nodulation was assessed. Strain USDA 110 was the most competitive, followed by USDA 138. Strain TAL 379 was always less competitive on both cultivars. The incidence of double-strain occupancy of nodules varied from 8% to 40%.  相似文献   

15.
Summary Previous laboratory and greenhouse studies have shown that phages significantly reduce soil populations of homologous rhizobia. Reductions in nodulation and N2 fixation have also been observed. The purpose of the current study was to examine the effect of a phage specific ofBradyrhizobium japonicum USDA 117 on nodulation, nodule occupancy, N2 fixation and soybean growth and yield under field conditions. The phage was inoculated in combination withB. japonicum USDA 117 and/orB. japonicum USDA 110 (resistant strain) into a rhizobia-free sandy loam soil and planted toGlycine max (L.) Merr. Williams. When the phage was applied to soil inoculated withB. japonicum USDA 117 alone, significant reductions in nodule weight and number, shoot weight, foliar N, nitrogenase activity, and seed index were observed. When, however, the soil also contained the non-homologous strain,B. japonicum USDA 110, no significant effects on any of these parameters were found. Nodule occupancy by competing strains ofB. japonicum USDA 110 and USDA 117 was also affected by the phage. In soil which did not contain the phage, 46% and 44% of the identified nodules were occupied by USDA 110 and 117, respectively. When the phage was present in the soil, nodule occupancy byB. japonicum USDA 117 was reduced to 23%, while occupancy byB. japonicum USDA 110 was increased to 71%. These results suggest that nodulation by selected strains of rhizobia can be restricted and nodulation by more effective, inoculated strains can be increased through the introduction of a homologous phage to soils.  相似文献   

16.
Summary Six fast-growing soybean rhizobia (Rhizobium fredii) and thirteen slow-growing soybean rhizobia (Bradyrhizobium japonicum) were examined for resistance to 10 antibiotics. Axenic studies were carried out to determine the competitiveness of dual-strain inocula consisting of fast- and slow-growing rhizobia isolated from subtropical-tropical soils for nodule occupancy on a hybrid of Asian and US soybean cultivars. Nodule occupancy was determined by intrinsic resistance to erythromycin and neomycin. The results showed wide variability in resistance to 10 antibiotics for fast- and slow-growing rhizobia. The intrinsic antibiotic resistance of fast- and slow-growing rhizobia was extremely high against nalidixic acid (400 g ml–1) and penicillin (200 g ml–1). The competitive ability of inoculant strains for nodule occupancy varied for different combination sets and with the plant growing media. Our results show that fast-growing rhizobia nodulate a hybrid of Asian and US soybean cultivars. Fast-growing soybean rhizobia did not completely exclude nodulation by the slow-growing strains, which formed 0–79% nodules, depending on the strain used in the inoculum.  相似文献   

17.
Bradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina.  相似文献   

18.
The competitiveness of two Bradyrhizobium japonicum strains was determined under controlled conditions. The relationship between the ratio of the nodules formed by the two bradyrhizobial strains and the ratio of the two strains in the inoculum was established and used as an internal standard to evaluate the equilibrium ratio between populations of the two strains in the soil. The fluorescent antibody (FA) technique was also used for counting the bradyrhizobial populations directly in the soil. Results with the FA technique were analysed and compared with the estimate obtained through nodule occupancy. The initial ratio of the two bradyrhizobial strains in populations introduced simultaneously into the soil changed during incubation time and the two strains reached the same equilibrium levels after 53 days. Never-theless, the strain introduced into the soil as an overpopulation remained clearly dominant for the nodule occupancy. We conclude that direct counts with the FA approach are selective and these counts could be mainly of the unattached bacteria.  相似文献   

19.
Abstract

Humic acids (HAs) obtained from four different soils by sequential extraction with pyrophosphate solutions at different pH values (i.e. at pH 3, 5, 7, 9, 11, and 13), were examined by ultraviolet and visible (UV-VIS) spectroscopy and elemental analysis. UV-VIS spectra from 230 to 700 nm were measured, and absorbance coefficients of 1% humic acid solutions at 600 nm (E 1% 600) and Δlog K were calculated. The shape of the spectra of humic acids depended on the soil types rather than on the pH values. The (E 1% 600) values were higher in the neutral region, and lower in the alkaline region, for each pH value they decreased in the following order: buried Andosol > Andosol > Histosol ≧ Cambisol. The Δlog K values for each pH value were higher in the following order: Histosol> Cambisol > buried Andosol≧Andosol. Although the linear correlation was found to be significant between the E 1% 600 and Δlog K values, the relation between these optical properties and pH values differed among the soil types. However, in the neutral region, the higher E 1% 600 values of humic acids from buried Andosol and Andosol and the lower Δlog K values of humic acids from Cambisol and Histosol suggest that the humification degree was higher in the neutral region. Data of elemental composition and atomic ratios were obtained for each humic acid. The H/C ratios in the four soils increased gradually with increasing pH values from 5 to 13, and those for each pH value were commonly higher in the following order: Cambisol> Histosol> Andosol> buried Andosol. The O/C and OIH ratios decreased with increasing pH values. The results of the H/C versus O/C diagram suggested that decarboxylated humic acids were extracted at the higher pH values. A highly significant correlation coefficient was obtained between the H/C and OIH ratios (r= ?0.891***). Results showing that the decrease of the pH values corresponded to the order of the humic acids in the correlation curve in the H/C versus O/H diagram suggest that the humic acids with a higher content of unsaturated bonds and a higher degree of oxidation were extracted at the lower pH values within the range from 5 to 13.  相似文献   

20.
Summary Experiments to assess the ability of free-living cells of six strains of soybean rhizobia (Bradyrhizobium japonicum USDA 76, 94, 110, 122, 123, and 135) to denitrify nitrate in five soils showed that although some strains ofB. japonicum have the capacity to rapidly denitrify nitrate in soils under anaerobic conditions, it is unlikely that the numbers of soybean rhizobia commonly found under field conditions are sufficient to significantly influence either the extent or the products of denitrification in soil. It is our general conclusion that the advantages, if any, that the ability to denitrify conveys to rhizobia or to the rhizobia-legume symbiosis are not offset by increased losses of plant-available N when denitrifying strains of rhizobia are present as free-living cells in soil.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号