共查询到19条相似文献，搜索用时 156 毫秒
1.
内蒙古地区奶牛病毒性腹泻/黏膜病血清流行病学调查 《畜牧与饲料科学》2014,35(3):106-106
应用ELLSA试验对来自内蒙古地区17个大﹑中﹑小型奶牛场的2391份牛血清样品进行了牛病毒性腹泻/黏膜病抗体检测,并对其中222份抗体阴性牛应用ELISA试验进行牛病毒性腹泻/黏膜病的抗原检测。结果表明：17个奶牛场均检出BVDV抗体阳性,共检出阳性血清2125份,阳性率最高达100%,最低为46.8%,平均为88.9%。对14个奶牛场进行了BVDV抗原检测,在5个奶牛场检出BVDV抗原阳性,阳性率为3.6%（8/222）。表明内蒙古地区奶牛场普遍存在牛病毒性腹泻/黏膜病感染,感染率较高,并且牛群中存在持续性感染（PI）牛。 相似文献
2.
3.
为了解某奶牛场牛群牛病毒性腹泻感染情况,随机对440份牛血清进行牛病毒性腹泻抗体和抗原检测。结果显示,抗体和抗原的阳性率分别为7.73%和0.68%,牛病毒性腹泻抗体/抗原阳性样品转换值的平均值分别为0.900和3.303,高于判定阳性的临界值,证实该奶牛场牛群确实存在牛病毒性腹泻感染。 相似文献
4.
5.
为了解湖南省规模牛场牛病毒性腹泻、牛传染性鼻气管炎、牛结核病、牛副结核病4种疫病的流行状况,采用ELISA方法,对全省未免疫以上4种疫病的规模牛场开展了血清学调查。结果显示：湖南省规模牛场的牛病毒性腹泻、牛传染性鼻气管炎、牛结核病、牛副结核病的场阳性率分别为51.35%、74.32%、13.64%、22.73%,个体阳性率分别为23.54%、45.45%、3.11%、4.67%。全省14个市州规模牛场均检出牛病毒性腹泻与牛传染性鼻气管炎病原抗体阳性,4个市州检出牛分枝杆菌抗体阳性,7个市州检出副结核分枝杆菌抗体阳性;不同规模牛场4种疫病的抗体阳性率存在一定差异,大规模场偏高;多重感染占比为45.45%。结果表明：牛病毒性腹泻和牛传染性鼻气管炎在湖南省规模牛场流行较为普遍、严重,部分地区还存在牛结核病、牛副结核病流行,而在大规模牛场4种疫病流行更为严重,各规模牛场4种疫病病原的多重感染情况也较为普遍。因此,湖南省规模牛场4种疫病防控形势严峻,需加强防控。本调查为湖南省此类疫病防控提供了数据参考。 相似文献
6.
为探讨河南省牛病毒性腹泻病毒的流行情况,用牛病毒性腹泻病毒抗体检测试剂盒(IDEXX)对本省部分地区6个奶牛场、5个肉牛养殖场的181份牛奶样品和395份血清样品进行BVD抗体的检测.结果检出阳性牛奶106份,阳性率最高为93.3％,最低的为5.0％,平均阳性率为58.6％.其中有5个规模化奶牛场牛奶样品阳性率均在75％以上.肉牛血清样品共检出阳性124份,最高阳性率为74.12％,最低5.80％,平均阳性率31.39％.结果表明,该病在所调查地区中奶牛和肉牛均有感染,不同牛场间感染率差异较大,其中规模化奶牛的感染率较高.表明应采取相应的净化措施对该病进行控制. 相似文献
7.
在对北京地区20个奶牛群进行抗体水平检测和全群抗原筛查的基础上,将牛群中牛病毒性腹泻病毒流行状况同牛群在3年内是否引入过动物进行相关性比较。结果显示,20个牛群中,引入过动物的8个牛群中后备牛群血清抗体平均水平为80.0%,抗原筛查的总体阳性率为0.61%;未引入过动物的12个牛群中后备牛血清抗体平均水平为65.5%,抗原筛查的总体阳性率为0.15%,两组数据之间差异极显著,表明牛群在3年内是否引进过动物对牛群持续感染牛的分布影响极显著,动物引进可能是造成BVDV在北京地区流行传播的主要原因之一。 相似文献
8.
用ELISA方法对三明市2016年随机抽取的牛群血清样品进行检测,检测内容包括牛病毒性腹泻、牛白血病、牛传染性鼻气管炎、牛布鲁氏菌病。本次调查共检测样品204份。其中检测规模场7个,样品116份;检测散养户14个,样品88份。结果表明：检测牛病毒性腹泻病毒血清204份,检出阳性血清65份;检测牛白血病血清188份,检出阳性血清34份;检测牛传染性鼻气管炎血清184份,检出阳性血清64份;检测牛布鲁氏菌病血清204份,检出阳性血清0份。说明三明地区牛群存在多种疫病感染,应采取相应的防控措施。 相似文献
9.
10.
针对某牛场奶牛流产率过高的状况,对该牛场流产情况及相关疾病进行诊断调查。该场2007—2009年奶牛年流产率分别为13.89%,16.52%和19.24%,流产率逐年上升。2009年1月至2010年4月的流产病例多发生于头胎牛,流产时妊娠时间以4～6月龄为主。收集流产奶牛病料24份。牛病毒性腹泻病毒血清抗体阳性率75%,犬新孢子虫血清抗体阳性率83.33%,其中9个病例为牛病毒性腹泻病毒与犬新孢子虫混合感染。布氏杆菌、李氏杆菌与牛传染性鼻气管炎病毒检测均为阴性。场内对照牛20头,牛病毒性腹泻血清抗体阳性率5.00%,犬新孢子虫抗体阳性率15.00%,与流产病例检测结果存在显著性差异。由此,可初步确定牛病毒性腹泻病毒合并犬新孢子虫感染是导致该牛场流产的主要原因。 相似文献
11.
12.
重庆市部分地区牛病毒性腹泻／粘膜病血清流行病学调查 总被引：1，自引：0，他引：1
为了解牛病毒性腹泻／粘膜病（BVD／MD）的流行情况,从重庆市辖区内11个区县（自治县）采集奶牛、黄牛、水牛血清共;369头份,用酶联免疫吸附试验（ELISA）检测BVD／MD抗体。结果从9个区县（自治县）检测出阳性样品,阳性率介于8．33％～50．00％之间,总阳性率为22．49％;规模奶牛场、散养奶牛、散养黄牛、散养水牛的阳性率分别为42．16％、9．62％、21．31％、9．76％。提示该病在我市各种牛群中存在,污染较广,应引起重视。 相似文献
13.
14.
Background
Bovine viral diarrhoea (BVD) is an infectious disease of cattle with a worldwide distribution. Herd-level prevalence varies among European Union (EU) member states, and prevalence information facilitates decision-making and monitoring of progress in control and eradication programmes. The primary objective of the present study was to address significant knowledge gaps regarding herd BVD seroprevalence (based on pooled sera) and control on Irish farms, including vaccine usage.Methods
Preliminary validation of an indirect BVD antibody ELISA test (Svanova, Biotech AB, Uppsala, Sweden) using pooled sera was a novel and important aspect of the present study. Serum pools were constructed from serum samples of known seropositivity and pools were analysed using the same test in laboratory replicates. The output from this indirect ELISA was expressed as a percentage positivity (PP) value. Results were used to guide selection of a proposed cut-off (PCO) PP. This indirect ELISA was applied to randomly constructed within-herd serum pools, in a cross-sectional study of a stratified random sample of 1,171 Irish dairy and beef cow herds in 2009, for which vaccination status was determined by telephone survey. The herd-level prevalence of BVD in Ireland (percentage positive herds) was estimated in non-vaccinating herds, where herds were classified positive when herd pool result exceeded PCO PP. Vaccinated herds were excluded because of the potential impact of vaccination on herd classification status. Comparison of herd-level classification was conducted in a subset of 111 non-vaccinating dairy herds using the same ELISA on bulk milk tank (BMT) samples. Associations between possible risk factors (herd size (quartiles)) and herd-level prevalence were determined using chi-squared analysis.Results
Receiver Operating Characteristics Analysis of replicate results in the preliminary validation study yielded an optimal cut-off PP (Proposed Cut-off percentage positivity - PCO PP) of 7.58%. This PCO PP gave a relative sensitivity (Se) and specificity (Sp) of 98.57% and 100% respectively, relative to the use of the ELISA on individual sera, and was chosen as the optimal cut-off since it resulted in maximization of the prevalence independent Youden’s Index.The herd-level BVD prevalence in non-vaccinating herds was 98.7% (95% CI - 98.3-99.5%) in the cross-sectional study with no significant difference between dairy and beef herds (98.3% vs 98.8%, respectively, p = 0.595).An agreement of 95.4% was found on Kappa analysis of herd serological classification when bulk milk and serum pool results were compared in non-vaccinating herds. 19.2 percent of farmers used BVDV vaccine; 81% of vaccinated herds were dairy. A significant association was found between seroprevalence (quartiles) and herd size (quartiles) (p < 0.01), though no association was found between herd size (quartiles) and herd-level classification based on PCO (p = 0.548).Conclusions
The results from this study indicate that the true herd-level seroprevalence to Bovine Virus Diarrhoea (BVD) virus in Ireland is approaching 100%. The results of the present study will assist with national policy development, particularly with respect to the national BVD eradication programme which commenced recently. 相似文献15.
Billinis C Leontides L Amiridis GS Spyrou V Kostoulas P Sofia M 《Preventive veterinary medicine》2005,72(1-2):75-9; discussion 215-9
Thirty-nine Greek dairy herds, totalling 6333 cattle, enrolled in a voluntary bovine viral diarrhoea virus (BVDV) eradication programme based on the identification and removal of persistently infected (PI) animals. The aim of this study was to estimate the prevalences of BVD antigen-positive and PI animals, and investigate the significance of the associations between the prevalence estimates and herd size. Initially, all animals were bled and examined for BVDV, using an antigen ELISA. A second sample was collected from the positive animals, after a period of at least three weeks. Animals retested positive were classified as PI. Antigen positive and PI animals were detected in all herds. The respective mean prevalences, adjusted for the test's accuracy and the herd-clustering effect, were 14% (95%CI: 11-18%) and 1.3% (0.8-1.8%), respectively. Herd size was not associated with the prevalence of antigen-positive or PI animals. 相似文献
16.
Camilla Luzzago Valerio Bronzo Stefano Salvetti Michela Frigerio Nicola Ferrari 《Veterinary research communications》2010,34(1):19-24
The herd seroprevalence of bovine respiratory syncytial virus (BRSV) was studied in 59 dairy cattle herds using serology on
random selected animals stratified by two age classes (heifers, cows). Risk factors for primary infections in heifers were
investigated using a questionnaire on management conditions and data on bovine viral diarrhoea (BVD) status. At least one
seropositive cow was present in all the herds. In 25% of the herds all individual were seropositive and 22% of herds had all
heifers seronegative. Analysis of the influence of risk factors retained summer pasture and BVD status. In particular, absence
of summer pasture and the BVD positive status of heifers were associated with an increased risk of BRSV infection in heifers
group. 相似文献
17.
为了解新疆地区部分规模奶牛场牛病毒性腹泻病（BVD）的流行情况,优化防控措施,以达到建设防控净化场的目的,自2020年11月到2021年7月累计采集新疆5 个地区16 个奶牛场共计26 997 份血清、3 843 份犊牛耳组织进行全群普检。通过使用IDEXX公司牛病毒性腹泻病毒（BVDV）抗原检测试剂盒检测及RT-PCR复检结合测序等方法,淘汰阳性牛,同源性分析流行毒株情况。BVDV血清抗原检测结果为：沙湾某奶牛场BVD阳性率为1.63%（38/2 326）;乌鲁木齐某奶牛场BVD阳性率为0.35%（4/1 132）;其余奶牛场均为阴性。犊牛耳组织抗原检测结果为：乌鲁木齐某牛场BVDV阳性率为1.17%（4/342）;其余奶牛场均为阴性。研究结果揭示,奶牛场通过淘汰BVDV阳性牛,调整免疫程序、制定消毒程序等方法,可有效净化BVD。 相似文献
18.
Rikula U Nuotio L Aaltonen T Ruoho O 《Preventive veterinary medicine》2005,72(1-2):139-42; discussion 215-9
The bovine viral diarrhoea virus (BVDV) situation among dairy herds and suckler-cow herds was monitored annually from 1998 to 2004. Bulk-tank milk (BTM) samples from all dairy herds and serum samples from beef animals at slaughter were examined for BVDV antibodies using a commercial indirect ELISA test. New BTM antibody-positive herds and herds with a history of BTM antibodies, but previously untested were sampled individually and tested for evidence of BVDV. The reason for the antibody-positivity or the source of infection was investigated. The percentage of BTM antibody-positive herds ranged from 0.45% in 2000 to 0.15% in 2003. The number of herds with persistently infected (PI) animals ranged from 10 in 2001 to 0 in 2003. The most common cause for a herd to become BTM antibody-positive was the purchase of a seropositive animal or a PI animal or a dam carrying a PI fetus. The new BVD decree of 2004 will be described in brief. 相似文献
19.
Nuotio L Juvonen M Neuvonen E Sihvonen L Husu-Kallio J 《Veterinary microbiology》1999,64(2-3):231-235
Bulk milk samples from every herd supplying milk to dairies in Finland were examined for the presence of antibodies to BVD virus (BVDV) annually during 1993-1997. The highest prevalence, 0.99% in 1994, declined to 0.37% in 1996; however, this favourable trend appeared to discontinue in 1997, where the prevalence remained at 0.41%. In 1993, sera of all individual animals from bulk milk antibody-positive herds were examined for the presence of these antibodies. Since 1994, only sera of animals from herds with a bulk milk absorbance reading greater than 0.250 in the EIA test were examined individually. Three geographic foci of BVDV antibody-positive dairy herds were resolved in 1994, one in the north-western, another in the eastern and a diffuse third in the southern part of Finland. A distinct limiting of the spread was apparent in 1997. Beef cattle were also studied during 1993-1997; in 1993 breeding units, in 1994 mainly beef suckler herds and in 1995-1997 serum samples of beef animals at slaughter were examined for the presence of antibodies to BVDV. The prevalence of seropositive herds in 1993 and 1994 was 30.2% and 3.2%, respectively, while the prevalence among slaughter animals ranged 0.8-1.6%. Seronegative animals in herds with > 50% of seropositive animals were examined for the presence of BVD-virus. A total of 40 dairy herds and two beef herds with viraemic (persistently infected, PI) animals was encountered during 1993-1997. A comprehensive control programme and a more specific, cooperatively funded eradication programme for dairy cattle were launched in 1994. These programmes most probably contributed to the decline in prevalence during 1994-1996. 相似文献