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花色苷是常见的水溶性天然食用色素,具有多种对人体健康有利的生物功效。但花色苷的稳定性较差,易受外界因素影响。简述了分子结构、pH值、温度、光照等因素对花色苷稳定性的影响,并进一步综述了花色苷有机酸酰化和黄酮辅色研究进展,为深入探究花色苷稳定化方法和扩大花色苷在食品加工中的应用提供借鉴。 相似文献
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蓝莓花色苷提取工艺及其抗氧化性研究 总被引:1,自引:0,他引:1
以蓝莓为原料,采用溶剂萃取法提取蓝莓花色苷,通过单因素和正交试验确定其最佳提取工艺条件。并以VC为对照,研究花色苷清除超氧阴离子自由基(O2-·)、DPPH自由基、羟自由基(·OH)和抗油脂氧化、还原力等抗氧化活性。结果表明,溶剂法提取蓝莓花色苷的最佳工艺条件为:提取溶剂80%乙醇,液料比15∶1(m L·g-1),p H 3,温度40℃,提取70 min,蓝莓花色苷的色价最高。蓝莓花色苷具有很好的抗氧化性,其抗氧化能力随浓度的增大而提高,花色苷对油脂的抗氧化能力比VC的效果稍好;花色苷的还原力低于同浓度的VC,VC的还原力是花色苷的1.7倍;0.1%和0.5%的花色苷对·OH的清除率高于相同浓度的VC,但是0.2%~0.4%浓度的VC比相同浓度花色苷的效果稍好;相同浓度下,VC对O2-·的清除能力明显好于花色苷,是花色苷的1.54倍;蓝莓花色苷对DPPH自由基的清除效果好于同浓度的VC,是VC的1.07倍。 相似文献
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《农产品加工.学刊》2020,(15)
花色苷是广泛存在于植物细胞液中的一类以黄酮核为基础的化合物,因具有抗氧化、抗肿瘤、保护视力等生物活性而被广泛应用于食品、医药和保健行业。但是,由于影响花色苷稳定性的因素较多,极大地限制了花色苷的加工与应用,因此广泛探究花色苷稳定性性质非常重要。通过综述影响花色苷稳定性的主要因素,重点关注提高花色苷稳定性的方法,同时阐述花色苷降解前后的生理功能变化,为提高花色苷的利用率提供参考。 相似文献
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以“圆蓝”兔眼蓝莓为试材,研究了0.5%壳聚糖(0.4%高分子壳聚糖+0.1%低分子壳聚糖)涂膜对常温贮藏蓝莓果实品质的影响。结果表明,在20℃常温条件下贮藏20d的过程中,壳聚糖涂膜可以有效减缓蓝莓果实感官品质及果实硬度的下降速度,降低腐烂率和失重率,保持较稳定的TSS和有机酸含量;抑制PPO活性的快速升高和MDA的大量积累:有效延长常温条件下的贮藏期,有利于保持采后蓝莓的品质。但对采后蓝莓果实中的花色苷含量无明显影响.对类黄酮的增加有一定的抑0作用。 相似文献
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套袋和抑制剂处理对桃果皮中葡萄糖-6-磷酸脱氢酶以及花色苷的影响 总被引:1,自引:0,他引:1
研究套袋处理和细胞G6PDH活性抑制剂-脱氢异雄酮(DHEA)涂抹处理,探讨桃果实着色过程中G6PDH、PAL活性与花色苷的关系。以‘早玉’桃(Prunus persica L. Batsch cv. ‘zaoyu’)为试材,研究了套袋对果皮花色苷、G6PDH和PAL活性、G6PDH亚细胞分布的影响,也试用DHEA处理,观察果皮颜色变化,并测定果皮中G6PDH活性和花色苷含量。自然生长的果实中,花色苷与G6PDH、PAL活性变化趋势相同;套袋果实摘除套袋后,G6PDH和PAL活性、花色苷都迅速增加,而未摘掉套袋的果实中,G6PDH、PAL活性和花色苷的含量基本没有变化;果实G6PDH主要存在于细胞质和质体(转色前为叶绿体,而转色期为杂色体)中,套袋使得叶绿体狭长,而且发生叶绿体裂殖;果皮质体中胶体金颗粒数量与G6PDH活性变化一致;脱氢异雄酮(DHEA)处理降低了G6PDH活性,花色苷含量也明显减少。套袋处理果实中G6PDH、PAL活性和花色苷有一定的相关关系,抑制G6PDH的活性能够抑制花色苷的合成。进一步表明G6PDH在果实花色苷合成中具有关键作用。 相似文献
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为研究紫薯花色苷提取方法及其稳定性,以紫薯为原料,吸光值或提取量为考察指标,在单因素试验的基础上,通过正交试验优化紫薯花色苷的提取工艺,并对花色苷稳定性进行了研究。结果表明,紫薯花色苷提取最佳工艺条件为:酸醇比1∶2(V/V),提取温度70℃,提取时间12 min,料液比1∶2(g/mL),此条件下花色苷吸光值为3.026。稳定性研究表明:花色苷对光敏感,在温度50℃以下和p H6的酸性条件下较稳定,耐还原能力比耐氧化能力强,大多数食品添加剂和金属离子Na~+、K~+、Ca~(2+)对花色苷溶液的稳定性影响不明显,但Fe~(3+)、Zn~(2+)对花色苷稳定性的影响较大。研究结果可为紫薯花色苷的提取和应用提供参考。 相似文献
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Inheritance of anthocyanins in berries of <Emphasis Type="Italic">Vitis vinifera</Emphasis> grapes 总被引:2,自引:2,他引:0
Zhenchang Liang Chunxiang Yang Jun Yang Benhong Wu Lijun Wang Jieshan Cheng Shaohua Li 《Euphytica》2009,167(1):113-125
Inheritance patterns for table grape anthocyanins were investigated on three cross offspring populations during two successive
years. Sixteen anthocyanins were detected, and all were monoglucoside derivatives. The proportion of anthocyanins in the maternal
parent determined the proportion of anthocyanins in the offspring. But the absolute content of the maternal parent had no
significant effect on progenies. Peonidin 3-O-glucoside and malvidin 3-O-glucoside were the most abundant anthocyanins, not only in the maternal parent but also in the progenies. The presence or
absence of anthocyanins in grape skin was inheritance of a quality character controlled by oligogenes, and anthocyanins content
was a quantitative character controlled by polygenes. Via principal component (PC) analysis, factors that affected the total
content of cross progeny populations were peonidin 3-O-glucoside, malvidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, petunidin 3-O-glucoside, peonidin 3-O-(6-O-coumaryl)-glucoside, and malvidin 3-O-(6-O-coumaryl)-glucoside. Anthocyanins content was a high broad sense heritability character (H2), and H2 was stable in different cross combinations (ranging from 0.65 to 0.98). 相似文献
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芜菁黄烷酮3-羟化酶基因的克隆、序列分析及表达 总被引:4,自引:0,他引:4
花青素是一类重要的植物次生代谢产物,黄烷酮3-羟化酶(F3H)是花青素生物合成早期阶段的重要催化酶。利用UV-A处理津田芜菁(Tsuda turnip)和赤丸芜菁(Yurugi Akamaru turnip)块根24h后提取总RNA,通过RT-PCR方法克隆了BrF3H基因。津田芜菁和赤丸芜菁的F3H基因完全相同。BrF3H的开放读码框为1077bp,编码358个氨基酸。氨基酸序列分析显示,BrF3H与甘蓝型油菜(Brassica napus)F3H-1的同源性为99%。Northern杂交结果显示,UV-A可以诱导BrF3H表达,基因的表达量与处理时间呈相关。 相似文献
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Sharifah N. Syed Jaafar Johanna Baron Susanne Siebenhandl‐Ehn Thomas Rosenau Stefan Böhmdorfer Heinrich Grausgruber 《Plant Breeding》2013,132(6):546-552
Due to their antioxidant activity, anthocyanins are of increasing interest for nutritionists, food scientists and plant breeders. Anthocyanins in wheat grains are expressed in either the pericarp or aleurone layer. Previous studies revealed that different anthocyanins are present in wheat varieties carrying genes for either the purple pericarp or the blue aleurone trait. Progeny from crosses between red‐, purple‐ and blue‐grained wheat varieties were selected over several cycles for grain colour by visual scoring. Bulked F5 grains were evaluated for their total anthocyanin content by UV‐VIS spectrophotometry and HPLC‐MS. The results demonstrate that it is possible to increase the anthocyanin content by the combination of the different genetic backgrounds for purple pericarp and blue aleurone, even though the majority of progeny were within the range of the purple‐ and blue‐grained check varieties. Visual scoring for grain colour is efficient, reliable and fast for selection in early breeding generations. Advanced breeding lines with high anthocyanin content can be identified by simple extraction methods and spectrophotometric measurements. 相似文献
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Chiara Lago Elena Cassani Cecilia Zanzi Michela Landoni Riccardo Trovato Roberto Pilu 《Plant Breeding》2014,133(2):210-217
Among the phytonutrients, anthocyanins have been extensively studied because of their antioxidant power, the characteristic supposedly responsible for their capacity for chronic disease prevention. Anthocyanins can also be synthesized in maize even though in Europe the colourless varieties have always been preferred. The aim of this study was to develop and characterize a new polenta variety of maize rich in anthocyanins, bred by a recurrent selection scheme, to increase the antioxidant power of this food. The recurrent selection was based on the anthocyanin content and other specific traits of the kernel. The coloured polenta obtained was analysed by TLC (Thin Layer Chromatography) analysis and DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) radical scavenging ability, before and after cooking. The results obtained showed that even though cooking reduced the anthocyanin content by about 22%, the remaining anthocyanins exhibited twofolds higher antioxidant capacity, expressed as ARP (antiradical power) using acetone/water extraction buffer in DPPH assay, compared to the colourless control. Furthermore, our data showed that the anthocyanin content did not alter the taste of the coloured polenta. 相似文献
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T. Yabuya 《Euphytica》1987,36(2):381-387
Summary Anthocyanins of colchicine-induced amphidiploids of I. laevigata × I. ensata were analyzed by the HPLC procedure and compared with those of parental species and F1 hybrids of I. laevigata × I. ensata. The amphidiploids were characterized by seventeen anthocyanins, and exhibited all anthocyanins of the parental species and the F1 hybrids. Malvidin 3RGac5G and petunidin 3RGac5G in anthocyanins detected were major pigments in the amphidiploids, which exhibited strongly the malvidin 3RGac5G-petunidin 3RGac5G type of I. ensata rather than the petunidin 3RGac5G-malvidin 3RGac5G type of I. laevigata. Moreover, a higher degree of anthocyanin content was observed in the amphidiploids and the F1 hybrids. Namely, the anthocyanin content of the amphidiploids and the F1 hybrids were 2.81 and 2.45 times as much as that of the mid-parent. This implies that there is a complementary effect on anthocyanin synthesis of parental genes in them. Finally, the utility of the amphidiploids was discussed.Contribution from the Laboratory of Plant Breeding, Faculty of Agriculture, Miyazaki Univ., No. 64. 相似文献
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《Postharvest Biology and Technology》2004,31(1):69-79
Graciano is a Spanish Vitis vinifera L. variety traditionally used to improve wine mixtures containing other original varieties. Given the scant literature on anthocyanins in Graciano grapes, the aim of this work was to determine its anthocyanin composition on the basis of HPLC/MS profiles and to compare it to those of other well-known varieties such as Tempranillo and Cabernet-Sauvignon. Thanks to this technique, the isomer cis of malvidin-3-(6-p-coumaroyl)-glucoside has been identified in the three varieties, being the first time this compound is reported in Vitis varieties. Anthocyanins in Graciano show a high proportion of peonidins, with peonidin-3-glucoside/malvidin-3-glucoside (PnG/MG) being considered as a potential marker for the characterization of the variety. However, the ratio between the sums of the p-coumaroyl/acetylated anthocyanins (ΣCm/ΣAc) allows the three varieties to be best distinguished, Tempranillo having the highest values followed by Graciano (intermediate) and Cabernet-Sauvignon (values of less than one). 相似文献
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黑果枸杞(Lycium ruthenicum Murry)中含有多种生物活性成分,如花青素类、多糖类、黄酮类、甜菜碱等,这些活性成分发挥抗肿瘤的药理作用机制目前已较为明确,其主要包括抑制肿瘤细胞增殖、诱导肿瘤细胞凋零、周期阻滞、肿瘤细胞分化及增强对肿瘤细胞免疫等作用。但目前关于黑果枸杞中主要抗肿瘤成分组成及对抑制肿瘤的转化机制和影响作用尚不明确。因此,综述了黑果枸杞中花青素、多糖、黄酮、甜菜碱等活性成分的研究现状及抗肿瘤机制,阐述了近年来有关黑果枸杞在抗肿瘤方面的研究进展,为黑果枸杞中功能性成分深入研究及精深加工提供参考依据。 相似文献