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1.
Tran Van Minh Nguyen Van Uyen Peter Vander Zaag 《American Journal of Potato Research》1990,67(11):789-797
Seed tubers are costly and often unavailable in the warm tropics. Alternative methods of growing potatoes include the use of cuttings. Vigorous mother plants are needed to produce large quantities of cuttings particularly under warmer temperatures. This paper reports the influence of temperature, media fertility and hormones on the growth of apical cuttings and their subsequent productivity as mother plants in Vietnam. Subsoil or sand and dried sieved cow manure at a ratio of 1:3 was best for rooting and establishing cuttings as mother plants under high temperatures (minimum/maximum of 23.7/31°C). Weekly foliar sprays of diluted Knop’s media or complete NPK improved growth of rooted cuttings. Mother plants produced the greatest number of cuttings at the mid and high elevation locations (800 and 1300 m above sea level) where it is possible throughout the year. At the lowland site, temperatures went beyond the critical level from April to October for mother plant survival and apical cutting production. Kinetin or GA3 did not significantly improve cutting production while their combination did improve productivity compared to the control. Mother plants were generally productive for four months after their establishment. 相似文献
2.
Ribavirin (virazole) treatment of cultured axillary bud tips (3-4 mm) was tested as a method of eradicating potato virus Y (PVY) and potato virus S (PVS) from two potato cultivars, Norchip and Desiree. Ribavirin treatment was phytotoxic at all concentrations tested, but cultivars treated at 5 mg/1 were visually similar to the nontreated control cultures after 20 weeks. The buds treated with ribavirin at 20 mg/1 had a survival rate of only 30–40%. Virus assays indicated that 2,74,82, and 89% of the plants were free of PVY, and 1, 71, 83 and 90% were free of PVS, 20 weeks following treatment with 0, 5, 10, and 20 mg/1, respectively. Virus assays indicated that 2, 74, 82, 89% of the plants were free of PVY, and 1, 71, 83 and 90% were free of PVS, 20 weeks following treatment with 0, 5, 10, and 20 mg/1 ribavirin treatment respectively for cultivar Norchip. Desiree cultivar assayed 2, 69, 80 and 86% free of PVY and 2, 74, 85 and 93% free of PVS, 20 weeks following treatment with 0, 5, 10, and 20 mg/1 ribavirin treatment respectively. 相似文献
3.
Ribavirin treatment of cultured potato shoot tips was tested as a means of eradicating potato virus X (PVX) from two potato cultivars. Shoot tips were cultured on liquid medium containing 0, 1, 10 or 100μg/ml ribavirin. Cultures were evaluated periodically for viability, and scored for vigor on a relative growth scale. Developed plantlets were assayed for PVX by transmission tests toGomphrena globosa. Ribavirin treatment was phytotoxic at all tested concentrations, and lethal to all cultivars treated at 100 μg/ml. Treatment also delayed plantlet development by up to 2 months at 1 and 10 μg/ml as compared with nontreated controls. PVX assays indicated that 80 and 83% of the plantlets were free of PVX following treatment with 10 μg/ml for cultivars Russet Burbank and Red McClure, respectively. Five and 6% of the plantlets developed from the 1 μg/ml treatment were PVX-free, whereas 0 and 2% of the controls were PVX-free for the same cultivars. Six to 8 months were required to develop plants from shoot-tip cultures treated with 10 μg/ml ribavirin. 相似文献
4.
Thirty-fourSolanum genotypes infected with one or more potato viruses were evaluated in anin vitro virus eradication system combining heat therapy and the antiviral chemical ribavirin. This system was compared within vitro heat therapy or ribavirin treatment alone. The effect of expiant size on virus eradication following therapy was also evaluated. Infected plantlets were established in ribavirin lacking and amended medium; half of the plantlets in each medium were subjected to a 30-day heat treatment (35 C/31 C, 4-hr alternating periods), while the remaining plantlets were kept at room temperature (23 C). Following therapy, plantlets were tested quantitatively (OD at A405) for the appropriate virus by enzyme linked immunosorbent assay (ELISA) and divided into 3 categories: low(< -0.05 OD), medium (> 0.05 to < 1.0 OD) and high (> -1.0 OD). Three plantlets were selected per group and further propagated as nodal cuttings or meristemtips. Regenerants which tested negative (< -0.05 OD) were transplanted to plug trays in a greenhouse and were retested after a 30-day grow-out period. Seventy-four and 91% of the genotypes yielded virus-free (VF) plants following propagation as nodal cuttings and meristem-tips, respectively. The combined therapies generated twice as many VF plants as thermotherapy alone and 5% and 15% more VF plants for nodal cutting and meristemtip derived plantlets, respectively, than for chemotherapy alone. Eleven of the 34 genotypes exhibited susceptibility to heat, but VF plants were obtained from survivors or by shortening the thermotherapy period. The efficiency of the system was enhanced by selecting plantlets for propagation which tested < -0.05 OD after treatment with 92%, 93%, 71%, and 100% of them remaining VF from potato viruses S, X, Y and leafroll, respectively. Twice as many VF plantlets were produced when treated plantlets were then propagated from meristem-tips than from nodal cuttings, but the rapid regeneration rate of plantlets from nodal cuttings makes it an attractive propagation method. Plantlet evaluation by ELISA after therapy to select plantlets with a high probability of producing VF plantlets and after further propagation to assure virus freedom are strongly recommended. 相似文献
5.
Summary Single leaf cuttings with their subtended axillary buds proved to be useful models to study maturation and senescence in potato
(Solanum tuberosum L.). Senescence was more rapid if the cultivar was early maturing or if plants were exposed to short days before cuttings
were taken. Rooting was decreased by short days before cutting; excision of the buried bud only partially overcame the effects
on senescence and rooting. High rates of N to mother plants in the field delayed senescence of cuttings in a way analogous
to the delayed senescence of field plants. The tuberization of cuttings from late maturing cultivars was decreased by high
rates of N. The color of crisps made from bud tubers was positively correlated with the color of crisps made from ordinary
tubers of the same cultivars. Sugar concentrations in bud tubers decreased as cuttings became more senescent and increased
after cold storage. Bud tubers harvested while ‘immature’ had greater concentrations of reducing sugars after 90 d at 10°C
than did ‘mature’ bud tubers.
Paper No. 878, Department of Vegetable Crops, Cornell University. This research was supported through HATCH project NYS161414,
a contributing project to Regional Project NC150, and through HATCH project NYS161407. 相似文献
6.
The productive potential of potatoes (Solanum tuberosum L. cvs. Norland, Superior, Norchip, and Kennebec) was assessed for life support systems being proposed for space stations and/or lunar colonies. Plants were grown in walk-in growth rooms for 15 weeks at 20 C under 12-, 16- and 20-h photoperiods of 400 μmol mt-2st-1 photosynthetic photon flux (PPF). Norland yielded the greatest tuber fresh weight, producing 2.3, 2.4, and 2.9 kg/plant under 12-, 16-, and 20-h photoperiods, respectively. The respective yields for the other cultivars under 12-, 16-, and 20-h were: Superior, 1.9, 1.5, and 1.8 kg/plant; Norchip, 1.8, 1.4, and 2.0 kg/plant; and Kennebec, 2.3, 0.2, and 0.8 kg/plant. Shoot and total plant biomass increased with lengthening photoperiods except for Kennebec, which showed increased shoot growth but no change in total growth with the longer photoperiods. Kennebec shoot growth under the 20-h photoperiod, and to some extent under 16-h, was noticeably stunted with shortened internodes. In addition, leaves of these plants showed mild chlorosis with rusty “flecking” of the surfaces. The harvest index (ratio of tuber yield/total biomass) was highest for all cultivars under the 12-h photoperiod, with a maximum of 0.69 for Norland. Similarly, the tuber yield per input of irradiant energy also was highest under 12-h for all cultivars. The tuber yield expressed on an area basis for the highest yielding treatment (Norland under 20-h) equaled 2.2 kg dry matter mt-2. Over 15 weeks this equates to a productivity of 20.7 g tuber dry matter mt-2 dayt-1. Assuming 3.73 kcal per g tuber dry matter and a daily human dietary requirement of 2800 kcal, then 36 m2 of potatoes could supply the daily energy requirement for one human. Potential for increasing productivity is discussed. 相似文献
7.
Response of ten potato cultivars to temperature as measured by chlorophyll fluorescencein vivo 总被引：1，自引：0，他引：1
Forty-day-old plants of ten cultivars of potato,Solanum tuberosum L. (Alpha, Atlantic, Bintje, Caribe, Kennebec, Red Pontiac, Russet Burbank, Sebago, Shepody and Superior), were placed into a controlled environment chamber held continuously at 35 C and their growth (Plastochron Index) and several chlorophyll fluorescence parameters (O, P, T, Fv and Fr) were measured after 1, 14, 21 and 28 days’ exposure. The cultivars were grouped according to heat tolerance based upon survival of three of four plants. The least heat tolerant group, surviving 14 days, included Atlantic, Bintje and Superior. The medium heat tolerant group, surviving 21 days’ exposure, included Kennebec, Red Pontiac and Sebago. The best heat tolerant group, surviving 28 days’ exposure, included Alpha, Caribe, Russet Burbank and Shepody. In addition, chlorophyll fluorescence parameters of six plants of each cultivar were also measured after 1 h at 5, 15, 25 and 35 C exposure in a second experiment. In all cases plants in the group with the least tolerance displayed less fluorescence than the medium or high heat tolerance plants, suggesting that plants with the least tolerance to high temperature exposure had less energy transfer through PSII (Photosystem II). These plants also had a rise in T at temperatures aboveca. 15 C. A decrease in Fv during growth at 35 C was a good indicator of foliar heat damage. Chlorophyll fluorescence of plant tissue in all three groups increased after short exposure of 1 h to temperatures below 15 C and also after continuous exposure to 35 C. At both ranges of temperature, damage was probably occurring to the thylakoid membranes which inhibited re-oxidation of PSII. Since the rate of response was different, chilling and high temperature apparently differ in how they alter the thylakoid membranes. 相似文献
8.
PVX infected plantlets from two potato cultivars grownin vitro with 0.3, 3, 30 or 300 ppm kinetin were exposed to temperatures of 28 or 35 C. After 3 wk, axillary buds were isolated and grown aseptically in organogenic media, followed by PVX testing by ELISA. The serological test was also run on whole plantlets at the end of the kinetin-temperature exposure. No donor plants exposed to 28 C nor the plantlets derived from their buds gave an ELISA (-) reaction, regardless of the kinetin content of the media or that of the cultivar. At 35 C the virus was suppressed to undetectable levels in several whole plantlets. In the cultivar Alpha, 2 out of 6 resulting plantlets after isolation of buds were virus-free in the presence of 3.0 mg/1 kinetin during the treatments. From Atzimba, about 15–40% of the regenerated plants were ELISA (-), without any relationship to the cytokinin content in the media. Heat had a stronger influence on virus elimination than kinetin and the results varied with the cultivar. 相似文献
9.
澳洲坚果扦插繁殖技术在生产中的应用 总被引：2，自引：0，他引：2
在南亚所苗圃对6个澳洲坚果品种和在西双版纳苗圃对9个澳洲坚果品种插穗，分别以WGD-1和IBA、ABT—1号、IAA等生长调节剂处理，进行扦插繁殖比较试验。结果表明：WGD—1、IBA、ABT-1号和IAA的合理使用均有显著促进澳洲坚果插穗生根的作用。WGD-18000mg/L处理的插穗成苗率显著高于8000mg/L IBA及ABT-1号、IAA处理：IBA 8000mg/L处理成苗率显著高于IAA；但IBA与ABT—1号之间，ABT—1号与IAA之间，差异不显著。经2个苗圃3a以上应用WGD—1配方生产扦插苗，成苗率达82．34％；品种344在版纳苗圃成苗率显著高于660、741，而344、246、O．C、788、800、H2之间差异不显著，但788、294扦插苗根系少，扦插难度较大：在常规条件下，我国植区的澳洲坚果扦插繁育期最宜在冬季，以长20～25cm皮色灰白木栓化枝作插穗为佳，带叶扦插，插后40～45 d所有插穗均有愈伤组织形成，生根高峰期在插后115～150 d之间，但品种间有差异。 相似文献
10.
《Industrial Crops and Products》2000,11(1):7-10
Grindelia chiloensis (Asteraceae) is a shrub native to Patagonia, Argentina, in the process of domestication as a source of resin to complement rosin production by pines. Vegetative propagation to multiply selected genotypes has produced breakthroughs in the cultivation of new crops like jojoba (Simmondsia chinesis). The only available way to propagate Grindelia is by transplanting seedlings or direct seeding. Both alternatives rely on selected varieties, a process that takes several years in an open-pollinated, self-incompatible species. The objective of this study was to generate a protocol for the vegetative propagation of G. chiloensis. Two experiments were carried out to evaluate the effect of the mother plant, the source of the cutting (apical or basal), and the concentration of indole butyric acid (IBA) on rooting success and root and above-ground biomass accumulation. A third study was conducted to evaluate the field survival of the propagated plants. Stems (15 cm) were cut from several mother plants growing in the field and stored in plastic containers with ice for about 4 h. Two types of cuttings were prepared from each stem: apical (upper 6–8 nodes) and basal (lower 6–8 nodes). A commercially available IBA source (Hormex, rooting powder, Brooker Chemical, Hollywood, CA) was used at six concentrations (0.1, 0.3, 0.8, 1.6, 3.0, and 4.5%). The cuttings were dipped in water and in the plant regulator and planted into speedlings filled with peat moss, vermiculite and sand (1:1:1, v/v/v). The speedlings were placed under a mist system in a greenhouse at 25°C. Rooting success and root weight were evaluated 30 days after the experiments were started. The position of the cutting, the IBA concentration, and the mother plant affected rooting success. None of the basal cuttings rooted even with IBA treatment. For the apical cuttings, IBA concentrations between 0.1 and 1.6% resulted in more than 64% rooting and the largest root mass (P<0.01). The control cuttings (0% IBA) did not produce adventitious roots. Accessions differed on rooting success. Transplanting survival varied between 25 and 100% depending on the clone. Vegetative propagation will allow the multiplication of Grindelia clones selected for their productive superiority. Traits such as resin content and composition, and regrowth after harvest are important characteristics to select for. 相似文献
11.
Tuber tissues of 123 commercial cultivars were tested for their ability to synthesize solamarine glycoalkaloids. Eleven cultivars including ‘Kennebec’ and ‘White Rose’ synthesized major concentrations of solamarines, ranging between 42 and 85% of total glycoalkaloid, when tuber slices were exposed to light during wound-healing. Tuber tissues of the other 112 cultivars did not synthesize solamarines, or they synthesized only trace concentrations of these unusual glycoalkaloids. Nine of the 11 solamarine-synthesizing cultivars have a common ancestor, USDA 96-56. This parental clone synthesizes major solamarine concentrations and it also carries the R1 gene for late blight resistance that it inherited fromSolatium demissum. Results of solamarine analyses of foliage from 47 USDA 96-56 selfed progeny suggest that this parental clone is the source of a major gene(s) for solamarines present in 9 of the commercial cultivars. However, there appeared to be an alternative source of a gene(s) for solamarines because ‘White Rose’, with onlyS. tuberosum ancestors, also synthesized major solamarine concentrations. There was no association between the R1 gene for late blight resistance and the ability to synthesize solamarines in 31 USDA 96-56 selfed progeny that were analyzed for both characters. 相似文献
12.
Bertha J. Rios Iriarte N. R. Thompson C. L. Bedford 《American Journal of Potato Research》1972,49(7):255-260
Th protein content of six potato cultivars, Russet Burbank and the numerically designated 58, 709, 711–3, 321–65 and 322–6, ranged from 5.5 to 8.7 g per 100 g. Similar results were obtained by both Kjeldahl and Biuret procedures. Amino acid analyses with a Beckman model 120C showed methionine to be the low level amino acid. Glutamic and aspartic acids were in higher amounts than the essential amino acids. The Chemical Score was similar to data of bio-assays by the meadow vole,Microtus pennsylvanicus. Cultivar 58 produced the greatest weight gain, but was only significantly higher than cultivars 709 and 711–3. Both weight gains and digestibility were increased by the inclusion of methionine in the diet. 相似文献
13.
Zoospore suspensions ofPhytophthora infestans applied to potato cultivars in the field with an exponential inoculum sprayer resulted in defoliation within a range of severity that was log-normal with respect to inoculum concentration. Regression lines for different cultivars differed in position and, depending upon moisture conditions, slope, indicating that the cultivars differed in resistance to penetration and to invasion of tissue by the pathogen. 相似文献
14.
15.
C. H. Livingston R. Lambert M. Kaufmann K. Knutson 《American Journal of Potato Research》1976,53(3):81-86
Two Julesburg sandy-loam fields in Weld County, Colorado, with a history of Corky Ringspot (CRS) caused by tobacco rattle virus (TRV) were injected with Telone-C as fall or spring applications and planted to potatoes. Deep soil sample cores taken from the experimental fields were planted toNicotiana tabacum L. var. Samsun serving as TRV bait and indicator plants. Systemic infection of bait plants and assay of the bait plant roots for TRV indicate that potential TRV inoculum was present which escaped the effects of fumigation treatments. TRV was found to be widely but erratically distributed throughout the experimental fields. The low incidence of CRS in tubers harvested from the treated fields suggests that fumigation may have short term benefits but potential inoculum exists which could lead to infection after the effects of fumigation have dissipated. The total yield of tubers was increased in the two cultivars planted (Norgold and Norland) in the fumigated fields receiving spring or fall applications. The yield of U.S. No 1 Grade potatoes in the Norland cultivar was increased significantly by both fall and spring fumigation. These yield increases were directly related to the reduced incidence ofVerticillium wilt observed in the fumigated soils and reported as a personal communication from unpublished data. 相似文献
16.
G. R. Benoit C. D. Stanley W. J. Grant D. B. Torrey 《American Journal of Potato Research》1983,60(7):489-501
A study was conducted to establish a growth rate response of potato plant (Solanum tuberosum L.) tops to exposure to 72-hr periods of six constant temperature treatments (10, 15, 20, 25, 30, 35°C) and to determine a thermal optimum for vegetative potato growth rates. The plants were maintained under “no stress” soil moisture conditions throughout the study. The temperature treatments were accomplished by inserting the plants into a growth chamber for 72 hours at selected temperatures. Maximum growth (as measured by percent change in leaf area) occurred at 25°C with temperature growth differences significant at the 5% level. Plant height measurements (percent change occurring during treatment time) showed maximum stem elongation at 30°C during an early growth stage and 25°C during stage 2 (4 weeks later). Predictive models developed from the data indicate maximum rates of leaf area increase in both growth stages occur at 24.7°C and that maximum rates of stem elongation occur at 31.3 and 27.4°C for growth stages 1 and 2, respectively. Leaf water potential and stomatal resistance measurements taken at the end of each treatment period indicated that no water stress occurred. 相似文献
17.
Kathy L. Flanders David W. Ragsdale Edward B. Radcliffe 《American Journal of Potato Research》1990,67(9):589-602
Use of enzyme-linked immunosorbent assay (ELISA) in detecting potato leafroll infections in field grown potato, cv. Russet Burbank, was studied from 1986 to 1988 at Rosemount, Minnesota. The objective was to determine relative reliability of current season foliage ELISA, tuber tissue ELISA, and tuber progeny foliage ELISA. Serological tests were most accurate when foliage of tuber progenies was tested. ELISA underestimated total leafroll infection when current season foliage from the inoculated plant was used, in those plants inoculated during late tuber bulking stage. Current season foliage ELISA tests using newly expanded terminal leaflets were more reliable than were tests using older leaflets. Leafroll infection was detected in the current season foliage and tuber progenies (tuber tissue as well as tuber progeny foliage) of some plants seven days after inoculation. Most current season foliage infections were detected by day 14–28 depending on year. Differences among years were most likely caused by variation in quality of virus source plants and numbers of vectors used in inoculation. ELISA tests on tuber tissue were almost as effective as ELISA tests on tuber progeny foliage in detecting potato leafroll 20 days after inoculation, but ELISA on tuber tissue substantially underestimated infection if plants were sampled earlier. Maximum percent tuber infection occurred 20 days or more after inoculation. Movement of the virus from the inoculated stem to other stems decreased with increased plant age at inoculation. Percent infected tubers declined with increased plant age at inoculation. Action thresholds developed for aphids in managing potato leafroll virus should take into account the temporal change in percent infected tubers. 相似文献
18.
The concentration of potato virus Y (PVY) was determined, using ELISA values (A405 nm), in twenty-six potato cultivars belonging to five resistance groups, grown in the field and in the greenhouse. On the basis of virus concentration, potato cultivars of group A, B, and C did not differ significantly and constitute the most susceptible group; those of group D and E differ significantly with each other and with group A, B, C, and constitute moderate and highly resistant groups, respectively. In the second year of infection, virus concentration was higher in each group, irrespective of resistance level. Thus, the infected plants of resistant groups, in a second year of growth, could be as rich sources of virus to aphids as plants from susceptible groups. 相似文献
19.
《Plant Production Science》2013,16(3):359-364
AbstractA priming method called sand priming was developed using sand as a priming solid matrix. The effect of sand priming on improving the field emergence performance of five super sweet corn cultivars was investigated. Sand priming significantly improved field emergence performance of all super sweet corn cultivars, and there was marked improvement by priming at 20ºC for 24 hr. After sand priming at 20ºC for 24 hr, field emergence percentage (FEP) of “Green Superman”, “Huatian 1”, “Yangtian 1”, “Mitian 8”, and “Chaotian 43” was increased by 52.1%, 37.5%, 38.0%, 40.9%, and 33.3%, respectively. Their field emergence speed (FES) was 2.3, 1.8, 2.0, 2.0, and 1.8 times of the control, respectively. To further elucidate the effect of sand priming on improving the field emergence performance of super sweet corn, we analyzed the membrane system integrity, α-amylase activity and protein content. Sand priming at 20ºC for 24 hr improved membrane system integrity and α-amylase activity in all super sweet corn cultivars. Furthermore, sand priming at 20ºC for 24 hr accelerated the degradation of embryo protein after 1 d germination in “Green Superman”. 相似文献
20.
An efficient and convenient procedure for the isolation of protoplasts fromSolanum etuberosum is described. Excised leaves of plants grownin vitro were preconditioned for 3 days in the dark at 4 C. A 16 h enzyme digestion followed with purification by flotation in Babcock bottles produced a mean yield of 12.5 × 106 protoplasts/g fresh weight. Protoplast viability as judged by morphology at the time of isolation was 89%. Liquid preculture for 1 day at 1 × 106 protoplasts/ml was used prior to plating at 1 × 105 protoplasts/ml and gave a mean survival of protoplasts of 43% after 8 days in culture. 相似文献