共查询到20条相似文献，搜索用时 88 毫秒
1.
选用８号新西兰白兔来制备牛生长激素抗独特型抗血清,初次免疫以完全弗氏佐剂乳化抗原,加强免疫用不完全旨氏佐剂乳化,最后一次加强注射后１０天采血,采用酶联免疫吸附试验来测定抗血清滴度和血清阻断率：３只兔血清抗体滴度分别为１：１２８０００,１：５１２０００和１：１２８０００,而血清阻断率在血清释稀率为１：２０００到１：２５６０００时处于２７．７％到９２．９％范围。随后用硫酸铵沉淀结合ＤＥＡＥ纤维素层析对 相似文献
2.
选用８只新西兰白兔来制备牛生长激素抗独特型抗血清，初次免疫以及弗氏佐剂乳化抗原，加强免疫用不完全弗氏佐剂乳化，最后一次加强注射后１０ｄ采血，采用酶联免疫吸附试验来测定抗血清滴度和血清阻断率。 相似文献
3.
牛重组型生长激素抗独特型抗体的产生与鉴定 总被引：2，自引：0，他引：2
利用纯化的兔抗牛生长激素抗体ＩｇＧ来免疫３只绵羊以制备抗独特型血清。间接ＥＬＩＳＡ法和琼脂糖双扩散法测定抗血清滴度分别达到或超过１：１２８０００和１：６４以上。之后用硫酸铵沉淀法和亲和层析法纯化抗独特型抗体。采用间接ＥＬＩＳＡ法来研究抗独特型抗体与第一抗体ＩｇＧ成分特异性结合及生长激素与第一抗体中ＩｇＧ结合的影响,结果显示出它不但能ＩｇＧ成分特异性结合。而且能与生长激素竞争性结合第一抗体ＩｇＧ成分 相似文献
4.
5.
《江西畜牧兽医杂志》2017,(5)
本试验主要采用鸡胚尿囊腔繁殖病毒的方法繁殖新城疫病毒(NDV),差速离心法对NDV进行纯化,用纯化的NDV作为抗原,与弗氏佐剂充分乳化后,免疫8周龄的Balb/C小鼠。首免弗氏完全佐剂,二免、三免、四次加免用弗氏不完全佐剂。四免后第14天取免疫小鼠血清,用血凝抑制试验HI和间接ELISA测定抗体效价,分别为1:128和1:6.4×104,获得抗体与NDV具有良好的特异性反应,与鸡传染性法氏囊病毒、鸡传染性支气管炎病毒、鸡流感病毒均无交叉反应性。 相似文献
6.
选取18只BALB／c小鼠，以不同抗原（交联牛生长激素或未交联牛生长激素），以不同剂量、不同免疫间隔分别进行免疫，皆完全弗氏佐剂乳化抗原，皮下多点注射，三次免疫后，检测小鼠血清效价，确定免疫方案。从取脾前五天起，分别腹腔注射牛生长激素50、75、100、125和150μg，然后进行细胞融合，得到了分泌牛生长激素单克隆抗体的杂交瘤两株，分别将两株杂交瘤细胞注入小鼠体内生产腹水，腹水效价均达1：10000（ELISA）。 相似文献
7.
本试验提取乳酸杆菌L.caseiZhang的DNA作佐剂,按每只鸡100ug剂量添加到新城疫油苗及禽流感病毒H5亚型油苗中。新城疫和禽流感试验分别设空白对照组(Ⅰ组)、无佐剂疫苗组(Ⅱ组)、细菌核酸佐剂组(Ⅲ组)、弗氏完全佐剂组(Ⅳ组)、弗氏完全佐剂核酸免疫协同组(Ⅴ组)5组进行免疫,分别于15和28日龄首免,两周后二免。二免后每周采样用血凝及血凝抑制法检测血清抗体水平,并用MTT法检测脾脏T淋巴细胞增殖反应。结果显示,添加核酸组鸡HI抗体和T淋巴细胞增殖反应均极显著高于单独免疫疫苗组(P<0.01);而免疫组均极显著高于空白对照组(P<0.01);细菌核酸组HI抗体略高于弗氏完全佐剂组,但T淋巴细胞转化率极显著高于弗氏完全佐剂组(P<0.01);同时弗氏完全佐剂核酸免疫协同组与核酸组无显著性差异。由此得出,乳酸杆菌L.caseiZhangDNA对新城疫油苗和禽流感病毒H5亚型油苗具有免疫增强作用;乳酸杆菌L.caseiZhangDNA与弗氏完全佐剂无明显的免疫协同作用。 相似文献
8.
二种方法检测猪传染性胃肠炎病毒抗体的比较 总被引：3，自引：0，他引：3
分别用血清中和试验和单克隆抗体阻断酶联免疫吸附试验对８１头美国进口猪轿清作猪传染性胃肠炎抗体检测。ＳＮ试验同７份阳性血清，检出率为８．６４％，Ｂ－ＥＬＩＳＡ试验检出７份ＰＲＣＶ抗体阳性血清，检出率为８．６４％，无ＴＧＥ阳性。ＳＮ试验检出７份ＴＧＥ抗体阳性血清与Ｂ－ＥＬＩＳＡ试验检出的７份ＰＲＣＶ抗体阳性血清完全重合。结果证明，应用单克隆抗体进行的Ｂ－ＥＬＩＳＡ可鉴别诊断ＴＧＥ与ＰＲＣＶ感染，优于Ｓ 相似文献
9.
10.
为研究奶牛S100A12蛋白功能提供一种灵敏、高效的免疫学检测试剂,将纯化好的S100A12蛋白分别与弗氏完全佐剂和弗氏不完全佐剂乳化制备成抗原,免疫新西兰大白兔制备S100A12多克隆抗血清,应用琼脂双扩散法、间接ELISA法和Western blot方法检测该抗体的效价及其特异性。结果表明,所制备的S100A12抗血清经琼扩法和间接ELISA法检测效价分别达到1∶8和1∶409 600,同时免疫印迹法证实该抗体能与S100A12蛋白特异性结合。本试验成功获得了特异性强、效价高的S100A12多克隆抗体,为进一步深入研究S100A12基因功能提供了有力工具。 相似文献
11.
TAO Xuan GU Yi-ren LIANG Yan YANG Xue-mei ZENG Kai CHEN Xiao-hui YANG Yue-kui ZHONG Zhi-jun LV Xue-bin 《中国畜牧兽医》2015,42(12):3288-3293
The growth hormone (GH) and growth hormone releasing hormone (GHRH) contents in serum of 3 weights (90,110 and 130 kg) of Chuanzang Black swine hybrid and DLY pigs were detected by enzyme-linked immune sorbent assay (ELISA),their relationship with cross sectional area of muscle fiber (CSA) and intramuscular fat (IMF) content were also analyzed.The results showed that the GH contents of Chuanzang Black swine hybrid and DLY pigs of 3 weights were 14.25,14.61,14.11 and 17.86,16.98,16.77 μg/L,respectively,while the GHRH contents of which were 22.88,23.98,24.33 and 27.72,27.47,28.39 μg/L,respectively.There was no significant difference in different weight of the same breeds (P >0.05) except the significant difference between 90 and 130 kg of DLY pigs (P<0.05).The GH and GHRH contents of Chuanzang Black swine hybrid were both significant lower than DLY pigs of the same bodyweight (P< 0.05) except GHRH content of 110 kg weight (P >0.05).Relevance analysis showed that GH and GHRH contents had a significant positive correlation with CSA (P< 0.05),and an extremely significant negative correlation with IMF content (P< 0.01),which prompted that GH and GHRH might regulate the growth of muscle fiber and IMF deposition through blood. 相似文献
12.
采用ELISA方法检测了3个不同体重梯度(90、110和130 kg)的川藏黑猪配套系和DLY猪血清中生长激素(GH)和生长激素释放激素(GHRH)的含量变化,并分析了其与肌纤维面积(CSA)和肌内脂肪(IMF)含量的关系。结果显示,90、110和130 kg体重梯度川藏黑猪配套系和DLY猪血清中GH含量分别为14.25、14.61、14.11及17.86、16.98、16.77 μg/L,GHRH含量分别为22.88、23.98、24.33及27.72、27.47、28.39 μg /L,除DLY猪90和130 kg血清中GH含量差异显著(P<0.05)外,品种内不同体重梯度间差异均不显著(P >0.05);不同猪种间,除110 kg梯度GHRH水平差异不显著(P >0.05)外,其余川藏黑猪配套系均显著低于DLY猪(P< 0.05)。相关性分析结果显示,GH、GHRH水平与CSA呈显著正相关(P< 0.05),与IMF含量呈极显著负相关(P< 0.01)。结果表明GH和GHRH可能通过血液对猪肌纤维的生长和肌内脂肪的沉积进行调控。 相似文献
13.
SHI Jian-zhong LIU Yi WANG Ze-yi SHEN Nan ZHANG Ning-fang CHENG Zhi-min GAO Peng-fei GUO Xiao-hong LI Bu-gao CAO Guo-qing 《中国畜牧兽医》2016,43(10):2655-2660
The purpose of this study was to understand the change trend of growth hormone (GH) and growth hormone receptor (GHR) in serum of Mashen and Large White pigs during the period from 0 to 6 months of age,and to analyze the influence of GH and GHR on growth rate.The method of ELISA was used to detect the concentration of GH and GHR in serum of Mashen and Large White pigs from 0 to 6 months of age.The results showed that the variation trends of GH in Mashen pig was roughly the same as in Large White pig,the GH concentration was increased with age increasing after birth and reached the peaks at 4 and 5 months of age for Mashen and Large White pigs,respectively,and then decreased gradually.The serum GH concentration in Mashen pig was a little greater than that in Large White pig at 3 and 4 months of age,on the contrary,the serum GH concentration in Large White pig was greater than that in Mashen pig at other months.During the period from 0 to 6 months of age,the difference of GHR concentration in serum was not significant in Mashen pig (P>0.05).In Large White pig,the serum GHR concentration at 1 month of age was lowest,and was significantly lower than that at 4 and 6 months of age (P<0.01;P<0.05).During the period of 0 to 2 months of age,the GHR concentration in Mashen pig was greater than that in Large White pig,but the difference was extremely significant only at 1 month of age (P<0.01).Conversely,the serum GHR concentration in Large White pig was greater than those in Mashen pig during the period from 3 to 6 month of age,there was significant difference at 4 and 6 months of age (P<0.05),and there was extremely significant at 5 months of age(P<0.01).The concentration of GH and GHR in serum was related to the developmental stages and genetic background of pig,and its change trend was in accordance with the trend of growth rate. 相似文献
14.
试验旨在探讨马身猪和大白猪血清中生长激素（growth hormone,GH）及其受体（growth hormone receptor,GHR）在生长发育过程中的变化规律,采用ELISA方法检测马身猪和大白猪0~6月龄血清中GH和GHR的浓度。结果表明,马身猪和大白猪在0~6月龄血清中GH浓度变化趋势基本相似,随着日龄的增长,血清中GH浓度呈逐渐上升的趋势,分别在4和5月龄时达到最高值,随后又逐渐降低,但与峰值无显著差异（P>0.05）,在3和4月龄时,马身猪血清中GH浓度略高于大白猪,而在其他月龄,大白猪血清中GH均高于马身猪;0~6月龄,马身猪血清中GHR浓度无显著差异（P>0.05）,大白猪血清中GHR的含量在1月龄时最低,与4月龄差异极显著（P<0.01）,与6月龄差异显著（P<0.05）,其他月龄间差异不显著（P>0.05）,0~2月龄,马身猪血清中GHR含量高于大白猪,但只有在1月龄时差异达极显著水平（P<0.01）;3~6月龄,大白猪血清中GHR含量高于马身猪,且在4和6月龄时差异显著（P<0.05）,5月龄时差异极显著（P<0.01）。血清中GH和GHR浓度与猪的发育阶段和遗传背景有关,其变化规律与猪生长速度的变化趋势相一致。 相似文献
15.
16.
17.
To investigate the effects of long-term growth hormone-releasing factor (GRF) administration on plasma growth hormone (GH), LH and progesterone and body weight gain in growing buffalo calves, 12 female Murrah buffaloes within the age group of 6-8 months of age were divided into two groups (treatment and control groups) of six each in such a way so that average body weights between the groups did not differ (p > 0.05). Control buffaloes were not given any hormonal treatment and treatment group buffaloes were treated with synthetic bovine GRF [bGRF (1-44)-NH(2)] at the rate of 10 microg/100 kg body weight intravenously at an interval of 15 days from week 6 (5-week pre-treatment period) till 18 injections were completed (week 6-42 treatment period) and thereafter, effect of exogenous GRF were observed for 10-week post-treatment period. Jugular blood samples were drawn twice a week at 3-4-day intervals for plasma GH, LH and progesterone quantification. Body weight of all animals was recorded twice a week. During pre-treatment period, mean plasma GH, LH and progesterone did not differ (p > 0.05) between the groups. But during treatment as well as post-treatment period, mean plasma GH levels were found to be significantly (p < 0.01) higher in treatment than control group of buffaloes. Administration of GRF for longer term sustained a higher level of plasma GH even after cessation of treatment. GRF-treated buffaloes attained higher (p < 0.01) body weight than the controls. Repeated GRF administration for long-term significantly (p < 0.01) increased plasma LH and progesterone. In conclusion, repeated long-term exogenous GRF administration induces and even enhances GH release without any sign of refractoriness. GRF may, therefore, be used to induce daily GH release without loss of responsiveness over an extended period of time in young growing female buffaloes and it may assist these animals to grow faster. 相似文献
18.
小型猪生长激素基因启动子区SNPs分析 总被引：4，自引：1，他引：3
利用PCR-SSCP方法检测4种小型猪（五指山猪、巴马猪、香猪和藏猪）和2种中大型猪（达兰猪和长白猪）生长激素（GH）基因的单核苷酸多态性。发现小型猪中有3处碱基突变发生在5′-调控区,对基因型和等位基因频率进行分析,4种小型猪生长激素5′-侧翼区基因位点中,等位基因A和D为优势等位基因,与达兰猪和长白猪中的分布差异显著（P〈0.05）。以上结果表明猪品种间的体型差异可能与GH基因5′-调控区的基因突变和前导肽中的氨基酸变异有关。 相似文献
19.
生长激素释放肽-6缓释微球的制备及其对动物生长的影响 总被引：3，自引：0，他引：3
以生物降解材料乳酸乙醇酸共聚物(PLGA,LA-GA 8515)为载体,采用复乳-液中蒸发法制备含生长激素释放肽-6的乳酸乙醇酸共聚物微球,并通过体外药物释放试验评价载药微球的释药特征.结果得到收率、粒径大小和分布及含药量均满意的微球,其中包封率为100%,平均体积径为3.45μm,收率为79%.在37℃、pH7.0的生理等渗缓冲液中,首日释药19.19%,其后以平均日释药3.23%的速度释药25 d.小鼠肌肉注射微球30 d后,累积增重比注射生长激素释放肽-6、生理盐水分别高22.56%(P<0.05)和53.17%(P<0.01).结果表明,生长激素释放肽-6乳酸乙醇酸共聚物微球有望发展成为新型长效制剂. 相似文献
20.
动物生长激素的研究现状 总被引：2，自引：0，他引：2
生长激素是动物脑垂体远侧部腺垂体合成并分泌的一种具有种属特异性的单链蛋白质类激素,它对动物的生长发育、繁殖等起着非常重要的作用。作者综述了生长激素的结构、生理、功能及其基因等方面的研究进展。 相似文献