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1.
Hydrogen peroxide (H2O2) treatment is an alternative for disinfection in aquaculture, which may be advantageous as it dissociates and disinfects while increasing water oxygen concentration. Yet, accurate dosing remains undeveloped in Recirculating Aquaculture Systems (RAS). Dosage requirements can depend on organic burden, stocking density, feeding frequency, salinity, temperature and biofilter performance. The present case study investigated the dual effect of H2O2 application for oxygen enrichment and disinfection when continuously applied to a RAS rearing European seabass. H2O2 addition equivalent to 2.4 and 15.8 H2O2 mg L−1 were applied for 4 h per day in three 5-days experiments. H2O2 was injected at the inlet of protein skimmer and/or the rearing tanks in or without combination with traditional disinfection methods. Water microbial load and oxygen saturation were determined, along with stress markers glucose and cortisol in blood plasma of fish. Doses of 15.8 mg L−1 H2O2 steadily increased oxygen levels in holding tank water from ∼50 % to over 100 % saturation while reducing microbial load (from 604.4 CFU ml−1 in the rearing tanks before dosing to 159.8 CFU ml−1 after application), achieving suitable conditions for commercial fish densities in RAS. The doses used had negligible impact on biofilter performance and did not affect the fish in terms of stress. Overall results indicate H2O2 is effective for disinfection and oxygenation of RAS systems when applied at appropriate dosage and we recommend the protein skimmer as the safest position in order to protect the bacterial community of the biofilters and the reared fish.  相似文献   

2.
Foam fractionation is often considered an ineffective way of removing organic matter from freshwater due to the low surface tension of the water. There is, however, a lack of studies testing foam fractionation efficiency in replicated freshwater recirculating aquaculture systems (RAS). Foam fractionation can be applied with or without ozone. Ozone is a strong oxidiser previously shown to improve water quality and protein skimmer efficiency. To test the efficiency of foam fractionation and ozonation (20 g O3 kg-1 feed) separately and in combination in freshwater RAS, a two-by-two factorial trial was conducted with each main factor at two levels (applied or not applied). Each treatment combination was carried out in triplicates using 12 replicated pilot scale RAS stocked with juvenile rainbow trout (Oncorhynchus mykiss) and operated at a feed loading of 1.66 kg feed m-3 make-up water. The trial lasted 8 weeks and samples were obtained once a week. Ozone applied by itself significantly reduced the number of particles (83%), bacterial activity (48%) and particulate BOD5 (5-days biochemical oxygen demand; 54%), and increased ultra violet transmittance (UVT; 43%) compared to the untreated control group. Foam fractionation by itself lead to significant reductions in particle numbers and volume (58% and 62%, respectively), turbidity (62%), bacterial activity (54%) and total BOD5 (51%). A combination of both treatments resulted in a significant additional improvement of important water quality variables, including a 75% reduction in total BOD5, 79% reduction in turbidity, 89% reduction in particle numbers and 90% reduction in bacterial activity compared to the control. The removal efficiencies were within the same range as those observed in previous studies conducted with foam fractionators in saltwater systems (with or without ozone), corroborating that foam fractionation may become a useful tool for controlling organic matter build-up and bacterial loads in freshwater RAS.  相似文献   

3.
By keeping tropical shrimp, like Litopenaeus vannamei, in recirculating aquaculture systems (RAS), valuable food for human consumption can be produced sustainable. L. vannamei tolerates low salinities, and therefore, the systems can operate under brackish water conditions. The stabilization of the microbial community in RAS might be difficult under high organic loads, and therefore, water treatment measures like UV irradiation or ozone application are commonly used for bacterial reduction. To investigate the impact of these measures, the effects of UV irradiation and ozone application were studied in small-scale brackish water RAS with a salinity of 15‰ stocked with L. vannamei. UV reactors with 7 and 9 W were used, and by ozonizers with a power of 5–50 mg/hr, the redox potential in the water was adjusted to 350 mV. Ozone had a stabilizing effect on the microbial composition in the water and on biofilms of tank surfaces and shrimp carapaces, prevented an increase of nitrite and accelerated the degradation of nitrate in the water. UV irradiation led to changes in the microbial composition and was less effective in optimizing the chemical water quality. Thus, the use of ozone could be recommended for water treatment in brackish water RAS for shrimp.  相似文献   

4.
This study proposes a new and simple assay that allows rapid assessment of microbial activity in water samples. The assay consists of standardized hydrogen peroxide (H2O2) addition to a water sample and subsequent spectrophotometric determination of H2O2 reduction over time. The H2O2 decomposition rate constant reflects the level of enzymatic activity from planktonic and particle-associated bacteria as well as algae and protozoans. The proof of concept was verified on water samples from recirculating aquaculture systems (RAS), showing that the vast majority of H2O2 decomposition was related to microbial activity. Only 3% of the total H2O2 decomposition was related to abiotic processes when 0.20 μm sterile filtered RAS water was compared with unfiltered RAS water. Planktonic bacteria (size range 0.20–1.6 μm) accounted for 16% of H2O2 decomposition, while bacterial aggregates, particle-associated bacteria and microbiota above 1.6 μm were responsible for the remaining 81%. H2O2 decomposition rate constants were positively correlated to BOD5 (r = 0.893; p < 0.001; n = 18) and to the number of 1–30 μm micro particles (r = 0.909; p < 0.001; n = 72) in RAS water, substantiating the biologically mediated decomposition processes in the water phase. The H2O2 decomposition assay thus represents a new alternative to existing methods that allows rapid (1–2 h) and simple quantification of microbial activity in fresh- and saltwater samples from aquaculture systems. Potential applications of the assay are discussed.  相似文献   

5.
A continuous water disinfection process can be used to prevent the introduction and accumulation of obligate and opportunistic fish pathogens in recirculating aquaculture systems (RAS), especially during a disease outbreak when the causative agent would otherwise proliferate within the system. To proactively prevent the accumulation of fish pathogens, ozonation and ultraviolet (UV) irradiation processes have been used separately or in combination to treat water in RAS before it returns to the fish culture tanks. The objective of the present study was to determine the process requirements necessary to disinfect the full RAS flow, using ozonation followed by UV irradiation, just before the flow was returned to the fish culture tank(s). We found that a proportional-integral (PI) feed-back control loop was able to automatically adjust the concentration of ozone (O3) generated in the oxygen feed gas (and thus added in the low head oxygenator) in order to maintain the dissolved O3 residual or ORP at a pre-selected set-point. We determined that it was easier and effective to continuously monitor and automatically control O3 dose using an oxidative reduction potential (ORP) probe (in comparison to a dissolved ozone probe) that was located at the outlet of the O3 contact chamber and immediately before water entered the UV irradiation unit. PI control at an ORP set-point of 450 and 525 mv and a dissolved O3 set-point of 20 ppb provided almost complete full-flow inactivation of heterotrophic bacteria plate counts (i.e., producing <1 cfu/mL) and improved water quality (especially color and %UVT) in a full-scale recirculating system. Achieving this level of treatment required adding a mean dose of approximately 29 ± 3 g O3 per kg feed. However, because water is treated and reused repeatedly in a water reuse system, the mean daily O3 demand required to maintain an ORP of 375–525 mV (or at 20 ppb dissolved O3) was 0.34–0.39 mg/L, which is nearly 10 times lower than what is typically required to disinfect surface water in a single pass treatment. These findings can be used to improve biosecurity and product quality planning by providing a means for continuous water disinfection in controlled intensive RAS.  相似文献   

6.
A pilot-scale wastewater treatment station was built and operated at a commercial recirculating aquaculture facility in order to initiate, characterize and optimize the operation of a treatment strategy for effluent recovery and reuse. The treatment train consisted of sedimentation, denitrification, ozonation, trickling filter treatment, and chemical flocculation. The study consisted of four different sets of treatment conditions, differentiated by alternative use of 6 or 4 lpm flow and recycling rates, ozone doses between 36.6 and 82.5 mg O3/l water, and 6- or 9-min ozonation time. The effects of treatment on solids and dissolved organic compounds are reported here. Over 70% of solids were removed by sedimentation under all experimental conditions. At the end of treatment, up to 99% of TSS was removed due to the combined action of ozonation and chemical flocculation. COD removal was not significantly different among experimental conditions by sedimentation (59.2–62.7%, p > 0.05), but was positively correlated with ozone dose (slope = 0.452, r2 = 0.99), yielding total COD removal η(CODt) of 19.8–40.7%. Of these amounts, 60.4–66.5% of COD was removed with foam, while the balance was mineralized. The ozone reactivity was 83.7% at a dose of 82.5 mg O3/l water. The ozone consumption coefficient Y(O3/CODox) for COD oxidized was 1.92–2.23 g/g O3 COD and 0.70–0.78 g O3/g COD when total COD removed was considered. Overall, 87.9–92.4% of COD was removed by the treatment train, to an average of 44 mg/l at the highest ozone dose, a value 3.3–3.9 times less than in fish tanks. Under the same conditions, cBOD5 was reduced by 88%, 3.8–4.1 times less than in fish tanks. The water’s biodegradability was increased by over 20%. DOC did not change significantly through the treatment train, and fluctuated through the system due to methanol addition to support denitrification. Work with the pilot station showed that the treatment strategy employed could support effective recovery and recycling of aquaculture effluent, although salts and refractory organics may accumulate in the system.  相似文献   

7.
Fungal infestation by water mold Saprolegnia spp. causes great losses in aquaculture and fish egg hatching. To find a safe and effective alternative for the fungal prevention, we studied continuous disinfection of the inlet water by UV irradiation and ozonation combined with low concentration hydrogen peroxide (H2O2) treatments in a rainbow trout (Oncorhynchus mykiss) egg hatching system. High dose of UV irradiation (400 mWs/cm2) of the inlet water decreased the mortality of rainbow trout eggs from the 77.3% to 14.3% in a 28 day trial. UV irradiation did not modify water quality parameters, while combination of UV irradiation and H2O2 caused up to fivefold increase in the formate levels, and combination of O3 and H2O2 caused even ten-fold increase in the acetate and formate levels. UV suppressed the gradual increase of the heterotrophic bacterial counts on the fish eggs. Based on the molecular profiling high dose of UV reduced the growth of some of the dominating bacterial groups and combination of UV and H2O2 had a distinctive effect on the overall bacterial community structure on the fish eggs.  相似文献   

8.
Three of the most commonly used veterinary antibiotics—enrofloxacin (ENR), sulfamethoxazole (SMX), and oxytetracycline (OTC)—were chosen as representative antibiotics for UV/H2O2 treatments. The objective was to determine the optimization of UV/H2O2 to remove antibiotics from aquaculture discharge water using response surface methodology. The degradation of the antibiotics was investigated under varying UV/H2O2 conditions in environments with different levels of pH, water matrices, humic acid, and constituent ions. The degradation results demonstrated that increasing the H2O2 dosage facilitated ENR degradation at a neutral pH while facilitating degradation of SMX and OTC at a slightly acidic pH. The optimum removal conditions for ENR, which was used in all influential effect experiments and the contact tank experiments, was obtained at 10 mM H2O2, a pretreated COD of 87.51 mg L−1, and an initial pH of 6.15. Among the tested anions, only the presence of Cl- showed slight positive effects on ENR degradation, due to the generation of secondary active radicals. During the reaction, the hydroxyl radical (OH) was present at a higher pH while singlet oxygen (1O2) was slightly present at a lower pH. The experimental results from H2O2 sequential addition indicated that freshly added H2O2 could quench the recently generated OH and therefore a high H2O2 concentration with frequent adding was not necessary. Our contact system reduced the ENR concentration in both the effluent reservoir and in the UV irradiation zone. The overall results supported the use of the UV/H2O2 system to treat remnant antibiotics in the discharge water.  相似文献   

9.
The immune response in rainbow trout fry against Flavobacterium psychrophilum was elucidated using an immersion‐based challenge with or without prior exposure to hydrogen peroxide (H2O2). Samples were taken from the head kidney 4, 48, 125 and 192 h after immersion, and the regulation of several genes was examined. Bacterial load was assessed based on the presence of 16S rRNA and correlated with gene expression, and the levels of specific antibodies in the blood were measured 50 days post‐infection. Separately, both H2O2 and F. psychrophilum influenced gene expression, and pre‐treatment with H2O2 influenced the response to infection with F. psychrophilum. Pre‐treatment with H2O2 also affected correlation between gene regulation and pathogen load for several genes. A delay in antibody production in H2O2‐treated fish in the early phase of infection was indicated, but H2O2 exposure did not affect antibody levels 50 days post‐infection. An increasing amount of F. psychrophilum 16S rRNA was found in the head kidneys of infected fish pre‐treated with H2O2 relative to the F. psychrophilum group. The results show that a single pre‐treatment with H2O2 impairs the response against F. psychrophilum and may intensify infection.  相似文献   

10.
When operating water recirculating systems (RAS) with high make-up water flushing rates in locations that have low alkalinity in the raw water, such as Norway, knowledge about the required RAS alkalinity concentration is important. Flushing RAS with make-up water containing low alkalinity washes out valuable base added to the RAS (as bicarbonate, hydroxide, or carbonate), which increases farm operating costs when high alkalinity concentrations are maintained; however, alkalinity must not be so low that it interferes with nitrification or pH stability. For these reasons, a study was designed to evaluate the effects of alkalinity on biofilter performance, and CO2 stripping during cascade aeration, within two replicate semi-commercial scale Atlantic salmon smolt RAS operated with moving bed biological filters. Alkalinity treatments of nominal 10, 70, and 200 mg/L as CaCO3 were maintained using a pH controller and chemical dosing pumps supplying sodium bicarbonate (NaHCO3). Each of the three treatments was replicated three times in each RAS. Both RAS were operated at each treatment level for 2 weeks; water quality sampling was conducted at the end of the second week. A constant feeding of 23 kg/day/RAS was provided every 1–2 h, and continuous lighting, which minimized diurnal fluctuations in water quality. RAS hydraulic retention time and water temperature were 4.3 days and 12.5 ± 0.5 °C, respectively, typical of smolt production RAS in Norway.It was found that a low nominal alkalinity (10 mg/L as CaCO3) led to a significantly higher steady-state TAN concentration, compared to when 70 or 200 mg/L alkalinity was used. The mean areal nitrification rate was higher at the lowest alkalinity; however, the mean TAN removal efficiency across the MBBR was not significantly affected by alkalinity treatment. The CO2 stripping efficiency showed only a tendency towards higher efficiency at the lowest alkalinity. In contrast, the relative fraction of total inorganic carbon that was removed from the RAS during CO2 stripping was much higher at a low alkalinity (10 mg/L) compared to the higher alkalinities (70 and 200 mg/L as CaCO3). Despite this, when calculating the total loss of inorganic carbon from RAS, it was found that the daily loss was about equal at 10, and 70 mg/L, whereas it was highest at 200 mg/L alkalinity. pH recordings demonstrated that the 10 mg/L alkalinity treatment resulted in the lowest system pH, the largest increase in [H+] across the fish culture tanks, as well as giving little response time in case of alkalinity dosing malfunction. Rapid pH changes under the relatively acidic conditions at 10 mg/L alkalinity may ultimately create fish health issues due to e.g. CO2 or if aluminium or other metals are present. In conclusion, Atlantic salmon smolt producers using soft water make-up sources should aim for 70 mg/L alkalinity considering the relatively low loss of inorganic carbon compared to 200 mg/L alkalinity, and the increased pH stability as well as reduced TAN concentration, compared to lower alkalinity concentrations.  相似文献   

11.

Gilthead sea bream (Sparus aurata) was raised in six individual recirculating aquaculture systems (RAS) whose biofilters’ performance was analyzed. Fish were fed with three different diets (a control diet, a fishmeal-based diet (FM), and a plant meal-based diet (VM)) and with three different feeding strategies (manual feeding to apparent satiation, automatic feeding with restricted ration, and auto-demand feeding). For every combination of diet and feeding strategy, the mean oxygen consumption, ammonia excretion, and ammonia removal rate were determined. Fish fed with the VM diet consumed the most oxygen (20.06?±?1.80 gO2 consumed kg?1 day?1). There were significant differences in ammonia excretion depending on the protein content and protein efficiency of the diet, as well as depending on feeding strategy, which in turn affected ammonia removal rates. Fish fed by auto-demand feeders led to the highest mean ammonia removal rate (0.10 gN-TAN removed m?2 biofiltration area day?1), while not leading to peaks of high ammonia concentration in water, which preserve fish welfare and growth.

  相似文献   

12.
This study was conducted to investigate the protective effect of L‐carnitine (LC) against H2O2‐induced oxidative stress in the fathead minnow muscle cell line (FHM). The FHM cells were stimulated with 1 mM H2O2 for 1 h after LC pre‐treatment, and the cell viability and the activity and mRNA relative expression of antioxidant enzyme were measured to assess the antioxidant properties of LC. The results showed that the toxic effect of H2O2 on the viability of FHM cells was both dose‐ and time‐dependent. Furthermore, the viability of the 0.01–1 LC mM groups was significantly higher than those of the 1 mM H2O2 group. L‐carnitine protected the cells from H2O2‐induced oxidative damage, which was demonstrated by a significant reduction in the malondialdehyde and reactive oxygen species levels and increases in the intracellular total glutathione levels and the activities of total superoxide dismutase, catalase, glutathione peroxidase (GPx) and gamma‐glutamyl‐cysteine synthetase (γ‐GCS) in FHM cells pre‐treated with LC for 6 h compared with the 1 mM H2O2 group. In addition, the mRNA relative expression levels of the γ‐GCS catalytic subunit and nuclear factor nuclear factor erythroid 2‐related factor 2 were significantly higher than those of the 1 mM H2O2 group. It could be concluded that LC exerts a beneficial antioxidant effect against oxidative stress induced by H2O2 in FHM cells and that the appropriate treatment is 0.1–1 mM for 6 h in this study.  相似文献   

13.
The current study combined P and N removal using organic flocculant chemicals and woodchip bioreactors in both freshwater and brackish water (7 ppm) recirculating aquaculture systems (RAS). The use of carbon (C) containing flocculant chemicals in the process was hypothesized to further stimulate C-demanding N removal (denitrification) in bioreactors. The trial of combined P and N removal consisted of four treatments: freshwater and brackish water RAS with and without the addition of supernatant from flocculation process to the woodchip reactor. Duplicate woodchip reactors were used per treatment and the trial was run for six weeks. 56% and 49% of P was removed from fresh and brackish sludge water, respectively. The nitrate-N (NO3-N) removal rate was improved in the treatment when supernatant from flocculation process was used together with RAS discharge water when compared against the control. In brackish water RAS, the improvement was more pronounced (from 6.6–16.5 g NO3-N m−3 d-1) than in freshwater RAS (from 5.1–6.5 NO3-N m−3 d-1). In the freshwater bioreactors using supernatant, N was largely discharged as a nitrite-N (NO2-N). High NO2-N concentrations in freshwater reactors allude to incomplete denitrification reactions taking place. The results suggest that the organic flocculants did provide an additional C source for denitrification, which improved the N-removal process. However, in freshwater RAS this might have been partly due to untargeted processes such as DNRA (dissimilatory nitrate reduction to ammonium), and/or insufficient denitrification reactions taking place (excessive NO2-N production).  相似文献   

14.
Diets incorporating different levels of corn gluten meal replacement using biofuel algae or Spirulina protein at 0%, 25%, 50%, 75% and 100% were evaluated for larval/juvenile stage of Nile tilapia (Oreochromis niloticus). Fish averaging 0.02 g were divided into groups of 50. There were three replicates per every dietary treatment that were fed one of six diets for 11 weeks. Corn gluten protein was replaced with algae on the protein basis. All diets were supplemented with 1.5% lysine and 0.5% methionine. The experimental diets were formulated to contain 37 ± 2.8% protein and 14 ± 4.3% lipid in the form of fish oil and soybean lecithin (phospholipids source). The results indicated that algae positively affected feed consumption and fish growth up to the 50% replacement and then performance was depressed. Significant differences in concentration of individual minerals (Al, Fe, Zn and Cu) in the whole fish body were found. Mineral composition of algae might have affected growth when diets which contained more than 75% of plant protein were replaced with microalgae. These findings suggest that up to 50% of dietary corn gluten meal protein can be replaced with microalgae which significantly enhance fish growth.  相似文献   

15.
The goal of this study was to develop and characterize a cell line from the caudal fin tissue of zebrafish and also its application as an in vitro model to study the effect of H2O2 in wound healing. Fibroblastic cell line was developed using explant culture method from caudal fin tissue of zebrafish and characterized. This cell line was named as DrF cell line. The DrF cells treated with 0–10 µM/ml H2O2 were tested for viability, proliferation and motility by MTT assay, trypan blue assay and chemotaxis assay, respectively. Among the different concentrations of H2O2, 4 µM was found to be nontoxic to study cell migration in in vitro scratch wound assay. Furthermore, the expression of proliferating cell nuclear antigen (PCNA) and chemokine receptor (CXCR4) genes was carried by qPCR. The cell survival, proliferation and migration were extremely enriched at 4 µM level of H2O2. We observed accelerated wound closure in DrF cells treated with H2O2. The qPCR results indicated that H2O2 markedly up‐regulated mRNA expression of PCNA and CXCR4. The findings from our study suggest that H2O2 at low levels promotes cell survival, proliferation, migration and wound healing in DrF cells.  相似文献   

16.
Formalin is applied in certain aquaculture systems to control parasites infestations as well as bacterial and fungal diseases. This study investigated the capacity of end-of-pipe denitrifying woodchip bioreactors to remove potentially harmful amounts of residual formaldehyde (FA) from aquaculture effluents. Formaldehyde was readily removed by experimental- and field-scale denitrifying woodchip bioreactors and the removal of FA was found to be a combination of an initial adsorption of FA to woodchip surfaces (52 ± 2.8 g FA/m3 woodchips) and microbial degradation. Volumetric FA removal rates reaching 261 ± 27 g FA/m3/d were found at FA inlet concentrations of 90 mg FA/L and hydraulic retention times (HRT) of 5 h. High FA removal efficiencies ranged from 88.3 ± 4.6–99.8 ± 0.2% found for FA inlet concentrations –up to 105 mg FA/L and HRTs between 3.4 and 15 h. Microbial FA degradation rates in woodchip bioreactors were positively correlated to temperature with a Q10 value of 2.27 and a corresponding Arrhenius temperature coefficient of 1.086 for the investigated temperature range of 7–23 °C. At a commercial, outdoor recirculating aquaculture system (RAS) three full-scale woodchip compartments, achieved an average volumetric FA removal rate of 29.4 ± 0.2 g FA/m3/d and a removal efficiency of 82.5 ± 0.8% during the first 24 h following addition of FA. The results demonstrated that woodchip bioreactors are efficient in removing residual FA from RAS effluents and that nitrate removal was transiently enhanced during FA removal.  相似文献   

17.
The selection of aerators and correct numbers can play an essential role in reducing the cost of production in aquaculture. The new generation aerators, namely spiral leaf, air‐jet, submersible and impeller, used in aquaculture, were assessed for its aeration efficiency and energy cost compared with the commonly used paddle wheel aerator. Of the aerators tested, the impeller had the highest aeration efficiency of 2.098 kg O2/kW hr, followed by paddle wheel with 1.436 kg O2/kW hr at 20‰ salinity. The spiral and air‐jet aerators had maximum aeration efficiency of 1.326 and 1.419 kg O2/kW hr, respectively, at 35‰ salinity. The submersible aerator was not efficient as its maximum efficiency was 0.380 kg O2/kW hr. The water flow by paddle wheel was 3 ft/sec and also provides better coverage than other types of aerators. The efficiency of aerators was high in optimum salinities (20‰ and 35‰) than the low or high saline condition. The average energy cost of shrimp pond aeration per hectare was lowest for impeller, followed by paddle wheel aerator. The study provided economic comparisons of vannamei culture using different aeration systems by keeping a uniform set of economic assumptions. Shrimp farms with impellors can give 14%–25% high returns across salinities, whereas spiral leaf can provide 5% high returns at 35‰ salinity. The combination of the type of aerators and the calculated use based on the salinity of the culture systems can result in energy‐saving and also a reduction in the production cost.  相似文献   

18.
The immune response and morphological changes in the gills of rainbow trout fry after immersion in hydrogen peroxide (H2O2), Flavobacterium psychrophilum or combined exposure were examined. The gills were sampled 4, 48, 125 and 192 h after exposure, and the regulation of expression of the following genes was investigated using qPCR: IgT, IgM, CD8, CD4, MHC I, MHC II, IL-4/13A, TcR-β, IL-10, IL-1β, IL-17, SAA and FoxP3. Bacteria were not observed in haematoxylin-and-eosin-stained gill tissue, but the presence of F. psychrophilum 16S rRNA was detected using qPCR. The 16S rRNA levels were correlated with gene expression. Although pretreatment with H2O2 before immersion in F. psychrophilum did not significantly alter the amount of bacteria found in the gill, the immune response was influenced: exposure to F. psychrophilum resulted in a negative correlation with expression of IL-17c1, MHC I and MHC II, while pretreatment with H2O2 resulted in a positive correlation with IL-4/13A and IgM. Exposure to either H2O2 or F. psychrophilum influenced the regulation of gene expression and damaged tissue. Exposure to both combined altered the immune response to infection and postponed healing of gill tissue.  相似文献   

19.
Hydrogen peroxide (H2O2) is a commonly used treatment for a range of parasitic diseases of marine finfish, including amoebic gill disease (AGD). While this treatment is partially effective at reducing parasite load, H2O2 can have detrimental effects on the host under certain conditions. Treatment temperature and dose concentration are two factors that are known to influence the toxicity of H2O2; however, their impact on the outcome of AGD treatment remains unclear. Here, we investigated the effects of treatment temperature (8, 12 or 16°C) and dose concentration (750, 1,000, 1,250 mg/L) on the efficacy of H2O2 to treat AGD. We demonstrated that a 20-min bath treatment of H2O2 at all doses reduced both parasite load and gross gill score significantly. Parasite load and gross gill score were lowest in the 1,000 mg/L treatment performed at 12°C. At the high dose and temperature combinations, H2O2 caused moderate gill damage and a significant increase in the plasma concentration of electrolytes (sodium, chloride and potassium). Taken together, our study demonstrates that higher H2O2 treatment temperatures can adversely affect the host and do not improve the effectiveness of the treatment.  相似文献   

20.
A step toward environmental sustainability of recirculat aquaculture systems (RAS) is implementation of single-sludge denitrification, a process eliminating nitrate from the aqueous environment while reducing the organic matter discharge simultaneously. Two 1700 L pilot-scale RAS systems each with a 85 L denitrification (DN) reactor treating discharged water and hydrolyzed solid waste were setup to test the kinetics of nitrate and COD removal. Nitrate removal and COD reduction efficiency was measured at two different DN-reactor sludge ages (high θX: 33–42 days and low θX: 17–23 days). Nitrate and total N (NO3− + NO2− + NH4+) removal of the treated effluent water ranged from 73–99% and 60–95% during the periods, respectively, corresponding to an overall maximum RAS nitrate removal of approximately 75%. The specific nitrate removal rate increased from 17 to 23 mg NO3−-N (g TVS d)−1 and the maximal potential DN rate (measured at laboratory ideal conditions) increased correspondingly from 64–68 mg NO3−-N (g TVS d)−1 to 247–294 mg NO3−-N (g TVS d)−1 at high and low θX, respectively. Quantification of denitrifiers in the DN-reactors by qPCR showed only minor differences upon the altered sludge removal practice. The hydrolysis unit improved the biodegradability of the solid waste by increasing volatile fatty acid COD content 74–76%. COD reductions in the DN-reactors were 64–70%. In conclusion, this study showed that single-sludge denitrification was a feasible way to reduce nitrate discharge from RAS, and higher DN rates were induced at lower sludge age/increased sludge removal regime. Improved control and optimization of reactor DN-activity may be achieved by further modifying reactor design and management scheme as indicated by the variation in and between the two DN-reactors.  相似文献   

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