共查询到19条相似文献，搜索用时 140 毫秒
1.
对喜马拉雅长叶松种子进行24小时GA3和H2O2浸泡预处理和15d的2-3℃低温预处理后,研究了在20℃,25℃和30℃萌发条件下,21个不同种源的喜马拉雅长叶松种子的萌发情况。结果表明,H2O2（1%v/v）和GA3（10mg/L）浸泡预处理,种子萌发率分别是82.39%和78.19%,而未经预处理的种子平均萌发率为70.79%。GA3和H2O2浸泡预处理分别使种子萌发时间缩短了8d和10d。在超过21天的20℃萌发条件下,湿冷处理提高了种子萌发率和缩短了萌发时间 而在25℃和30℃萌发条件下,总的萌发率未受到影响。喜马拉雅长叶松种子因缺少休眠而表现出很好的萌发,但是因为越来越多的造林项目需要大量的种子,播种预处理有利于提高种子萌发率和萌发速率,有助于满足种子需求。 相似文献
2.
3.
以砂生槐种子为材料,利用清水、300mg.L- 1和600mg.L- 1GA3浸泡种子2d、4d和8d,探讨不同处理对砂生槐 种子萌发特性的影响。结果表明,随着浸泡时间的延长,砂生槐种子的发芽率有所提高,而且4~8d的300mg.L- 1 GA3 浸种可显著提高砂生槐种子的发芽率,相比清水浸泡发芽率提高10%~15%。 对于发芽势,清水、300mg.L- 1及600mg .L- 13种处理在浸泡2d和8d后只有 一个发芽势高峰,而浸泡4d后有两个发芽势高峰。 同时,方差分析结果表明,发芽 率在9种不同处理间呈极显著差异,腐烂率呈显著差异,但硬实率在不同处理间没有显著差异。 相似文献
4.
温度及浸种对杜鹃种子萌发特性的影响 总被引：1，自引：0，他引：1
《福建林业科技》2016,(4)
研究温度、浸泡液pH、赤霉素(GA3)及噻苯隆(TDZ)等处理对映山红(R.simsii)和马银花(R.ovatum)种子萌发特性的影响。结果表明:25℃/15℃变温条件有利于映山红种子的萌发;马银花种子在15℃恒温条件下发芽率达到最大值。GA3100 mg·L~(-1)处理促进映山红种子的萌发,但对马银花的种子发芽率影响不显著;不同浓度的TDZ溶液对马银花、映山红种子发芽率的影响差异均不显著;pH 4.5的溶液浸泡马银花种子,种子发芽率达到最大值,pH 6的溶液浸泡映山红,种子发芽率最好。综合试验结果表明,最适宜的发芽条件为:映山红种子用GA3100 mg·L~(-1)+TDZ 0.001 mg·L~(-1)、pH 6的溶液浸种,在温度为15℃的发芽箱中培养;马银花种子用GA3100 mg·L~(-1)+TDZ 0.001 mg·L~(-1)、pH 4.5的浸泡液中浸种,在25℃温度的发芽箱中培养。 相似文献
5.
6.
为找出最优育种方法,提高发芽率,获取更多优质种苗,从而为漆树种质资源及产业发展提供指导,采用三因素(98%的H_2SO_4、400 mg/L的GA3、砂藏时间)三水平的全因素试验方法,每处理设置单个变量,共计27个处理组合,对不同种源漆树种子萌发条件进行研究。结果表明:A种源漆树种子的最佳萌发条件为,98%的H_2SO_4处理30 min+400 mg/L GA3处理60 min+砂藏30 d;B种源漆树种子萌发率最高的条件为,98%的H2SO4处理30 min+400 mg/L GA3处理60 min+砂藏20 d;C种源漆树种子萌发率最高的条件为,98%的H_2SO_4处理30 min+400 mg/L GA3处理120 min+砂藏20 d。98%的H2SO4处理时间、400 mg/L GA3处理时间、砂藏时间对不同种源的漆树种子发芽率、芽长、芽粗均存在极显著影响。 相似文献
7.
大花黄牡丹种子休眠的解除 总被引：4，自引：0，他引：4
以9月采收于西藏林芝县、米林县并经自然晾干、室内储存3个月的大花黄牡丹种子为材料,研究温度、GAs、乙烯利等处理对种子休眠的解除效应。结果表明:1)经7天常温清水浸泡后直接播种于露地苗床,种子于第140天发根,第288天发芽,至发芽结束共490天,发芽率35%左右。2)温度对大花黄牡丹种子萌发具显著影响,低于10℃及高于25℃培养的种子均不能发根,15℃对下胚轴休眠解除效果最佳;15℃及20℃对上胚轴休眠解除效果最理想。3)各处理与对照的发根率均可达96%以上。4)GA3处理大花黄牡丹生根种子可起到显著的上胚轴休眠解除效果,其中以300mg·L-1处理效果最佳。5)综合考虑上胚轴及下胚轴休眠解除,理想的种子萌发条件为:播种前常温清水浸泡7天,于15℃恒温培养;待根生长至3cm以上时,以300mg·L-1GA3浸泡2h,置于15℃恒温培养。按此处理,发芽历期约120天,发芽率达95%以上,分别较常规播种缩短370天和提高171.4%以上。 相似文献
8.
珍稀濒危植物连香树种子萌发的研究 总被引：10，自引：0，他引：10
进行了濒危植物连香树种子萌发特性的研究,探讨不同光照条件(12 h,1 500 lux和黑暗)、不同温度(15℃、20℃、25℃和30℃)、不同浓度的赤霉素(0.5 mg.L-1、1 mg.L-1、2 mg.L-1、4 mg.L-1)浸泡连香树种子不同时间(2 h、4 h6、h、8 h)、50℃温水预处理15 min以及不同储藏条件对连香树种子萌发的影响。结果表明:25℃是连香树种子发芽的最适温度;在光照条件下连香树种子的发芽势高,光照可以促进连香树种子发芽迅速、整齐;经过赤霉素处理的干藏种子的发芽率和发芽势比没经过其处理的干藏种子的发芽率和发芽势高,且在一定浓度范围内随着赤霉素浓度的增大和处理时间的延长,发芽率和发芽势逐渐提高;湿藏有助于连香树种子萌发;50℃温水预处理15 min对种子的萌发有促进作用。 相似文献
9.
10.
《河北林业科技》2017,(2)
通过5组不同温度处理和赤霉素(GA3)、聚乙二醇(PEG)、细胞分裂素(6-BA)、磷酸二氢钾(KH_2PO_4)处理,研究不同条件对天人菊种子萌发特性的影响。结果表明:在变温25/15℃处理下,种子发芽率、发芽势、发芽指数最高,分别为83.67%、72.83%、6.88。由此可得:变温25/15℃是天人菊种子萌发的最适温度。在25℃恒温处理下,分别施用不同的激素,得到天人菊种子最适发芽浓度分别为300mg/L赤霉素(GA3)、10%PEG、100mg/L细胞分裂素(6-BA),但KH_2PO_4对天人菊种子萌发的影响并不显著。由试验可得:100mg/L的细胞分裂素(6-BA)对天人菊种子的萌发有更好的促进作用。 相似文献
11.
For improving seed germination of Prosopis koelziana and Prosopis juliflora, different treatments of seeds were conducted, including scarification with sulfuric acid 98% for 10 and 15 min, sandy paper,
hot water for 5 and 10 min, potasium nitrate 0.1%, gibberellic acid at 250 mg·L−1 and 500 mg·L−1 and combinational treatment of scarification with gibberellic acid of 250 mg·L−1 and 500 mg·L−1. The results show that scarifications with sandy paper and sulfuric acids 98% were the most effective treatments on breaking
seed dormancy and seed germination induction. Scarification with sulfuric acid 98% for 15 min was the best treatment. According
to the positive effect of scarification and lack of reaction of seeds against KNO3 and gibberellic acid, the kind of seed dormancy was determined as exogenous. 相似文献
12.
Cottonhead windhairdaisy (Saussurea laniceps Hand.-Mazz.) is one of the most famous and important medicinal herbs in China. Illegal collection from wild populations is increasingly threatening the present environment of S. laniceps. Establishment of an efficient method for micropropagation is the best way to change its endangered situation. When mature seeds of S. laniceps were cultured on hormone-free MS medium, plantlets were formed from germinated seeds in 7–10 d. Then 0.5 cm × 0.5 cm leaf explants were transplanted to MS medium supplemented with 1-naphthalene-acetic acid (NAA)/2,4-D and benzyladenine (BA)/KT and callus was achieved 10 d after transfer. Shoot bud regeneration occurred from callus cultured on MS medium supplemented with different growth regulators 20 d after culturing. The regeneration percentages varied with the different components of plant growth regulators. The percent regeneration from callus pretreated at low temperature of 5°C increased significantly compared with those incubated at 23/20°C directly. Optimal regeneration was observed with explants on media supplemented with 1.5 mg•L–1 BA plus 0.2 mg•L-1 NAA. In the presence of 0.2 mg•L–1 NAA in half-strength MS, 78% of the shoots formed roots. Plantlets from explants showed 63% survival after acclimatization. 相似文献
13.
We investigated the effect of provenance variation on cone and seed morphology and germination behaviour under different pre- treatments in Picea smithiana. Three categories of cones were recognized: large (13.18 cm × 3.30 cm 15.42 cm × 4.35 cm), medium (10.85 cm × 3.93 cm 12.18 cm × 3.98 cm) and small (7.69 cm × 3.06 10.98 cm× 3.39 cm). Significant variation was observed for various cone and seed characteristics. Seeds moisture content varied from 44.48% to 56.91%, seed size from 0.64 cm × 0.31 cm (largest seed) to 0.49 cm × 0.10 cm (smallest seed), and the seed weight from 2.45 g per 100 seeds to 1.36 g per 100 seeds on fresh weight basis of P. smithiana. The highest seed germination (72.0±7.53%) at 10°C was observed under chilling treatment in Tapovan provenance, while the minimum (15.0±5.71%) seed germina- tion was recorded at 25°C under control set in Tapovan provenance. Further among all the sources Pandukeshwar consistently had the highest average (38%) percent germination across all treatments. Compared to other temperature regimes and pretreatments, seeds subjected to 10°C and chilling treatment had the highest germination and took the least time for germination irrespective of provenance effects. Altitude (provenance) had little or no relationship with germination following laboratory treat- ments. Seeds from Tapovan provenance subjected at 10°C following chilling treatment may be suggested for further multiplication of this species. 相似文献
14.
Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L^-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L^-1 2,4-D. The best subculture medium was MS ＋ 0.8 mg＇L-1 2,4-D ＋ 30 g·L^-1 sucrose with a subculture cycle of seven days. 相似文献
15.
Leucaena leucocephala and Acacia farnesiana are tree species used for several agricultural purposes in the Mediterranean region. The seeds of these species exhibit dormancy,
causing delayed germination. Two experiments were conducted to investigate the effect of pre-sowing treatments (scarification,
hot water, or soaking) on seed germination of L. leucocephala and A. farnesiana. In one experiment, seeds were exposed to three pre-sowing treatments: control, sandpaper scarification, or soaking in 70°C
water for 4, 8, 12, 16, 20, or 24 min. In another experiment, seeds were soaked in 70°C water for 20 min, and then soaked
in water at room temperature for an additional 24, 48, or 72 h or blade scarified. In general, soaking the seeds of the two
species in hot water was more effective in breaking seed dormancy than scarification. Sandpaper scarification was not effective
for either species. Blade scarification increased A. farnesiana seed germination to 56%, indicating that seed dormancy was mainly a consequence of hardseededness.
L. leucocephala seeds collected from Jordan University of Science and Technology (JUST) site and soaked in 70°C water for 20 min and then
soaked for 24, 48, or 72 h had germination rates above 97%. Our results suggest that blade scarification of A. farnesiana seeds and soaking of L. leucocephala seeds in 70°C water for 20 min are effective treatments to break seed dormancy and enhance seed germination of these vital
species. 相似文献
16.
The AhDREB1 gene, cloned from Atriplex hortensis L., was transferred into black locust （Robiniapseudoacacia L.） by an Agrobacterium-mediated transformation. The results suggest that stems of black locust sub-cultured in vitro for 20 d are suitable for genetic transformation. The optimum concentrations of kanamycin and cefotaxime were 30 and 150 mg.L-1, respectively. Important factors affecting the transformation efficiency were studied by means of a L9（3^4） orthogonal design. An effective system for genetic transformation in black locust was developed as follows： the stems were pre-cultured for 2 d, immersed in the Agrobacterium solution （OD600 = 0.7） with 10 mg·L^-1 acetosyringone for 21 min and then co-cultured for 2 d. The selection pressures, changing from low to high, could improve transformation efficiency. The transgenic plants were identified by a PCR method. The PCR results indicated that the AhDREB1 gene had been integrated into the genome of black locust and two lines of the transgenic plants were obtained. 相似文献
17.
Effect of priming on the germination of<Emphasis Type="Italic">Peltophorum dubium</Emphasis> seeds under water stress 总被引：1，自引：0，他引：1
ZHANGWan-li LILe-ihong ZUYuan-gangt SoniaPerez 《林业研究》2004,15(4):287-290
Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 rain to overcome mechanical dormancy. Seeds were primed in solutions of 0.2% Captan at 10℃ and 27℃, PEG 6000 -1.0 MPa at 10℃ and 27℃, 0.5 mol KNO3, 0.75 Mol KNO3, 1.0 Mol KNO3. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27℃. For the experiments of all the groups, osmotic potential were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2, and -1.4 MPa. P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to -1.0 MPa.Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG -1.4 MPa. 相似文献
18.
Nursery practice using microbial inoculants was performed to find out the efficacy of the inoculants on seed germination and
seedling growth of sissoo (Dalbergia sissoo Roxb.). Microbial inoculants or effective microorganisms (EM) are a mixture of many different beneficial microorganisms in
a solution. The seedlings were grown in a mixture of sandy soil and cowdung (3:1) kept in polybags with pouring EM solution
at different concentrations (0.1%, 0.5%, 1%, 2%, 5% and 10%) before and after a week of sowing the seeds. Seed germination
rate and growth parameters of seedlings were measured, such as, shoot and root length, vigor index, fresh and dry weight of
shoot and root and total biomass increment. The nodulation status influenced by EM was also observed along with the measurement
of pigment contents in leaves. The highest germination rate (69%) was observed in 2% EM treatment, followed by 67% and 65%
in 1% and 5% EM. The highest shoot length (33.2 cm) was in 2% EM, whereas highest root length (26.3 cm) was in 1% EM. Both
fresh and dry weights from shoot and root, were maximum (4.16 g and 1.57 g; 2.12 g and 0.83 g respectively) in 2% EM and were
significantly (p ≤ 0.05) different from control. Vigor index was highest (4071) in 2% EM, which was significantly (p ≤ 0.05) different from control. Total dry weight increment was highest in 2% EM treatment, followed by 1% and 5% concentrations
of EM. Nodulation number was higher at very low (0.1%) concentration of EM solution but it normally decreased with the increase
of EM concentrations. The contents of chlorophyll a, chlorophyll b and carotenoid were highest (60.11, 17.05 and 42.48 mg·L−1 respectively) in 2% EM treatment and lowest (39.35, 13.55 and 27.29 mg·L−1 respectively) in control treatment. Therefore, low concentration of EM (up to 2%) can be used for getting maximum seed germination
rate and seedling development of Dalbergia sissoo Roxb. 相似文献
19.
We measured physiological parameters including water uptake, in-vitro embryo germination ratio, and seed coat structure observed by scanning electron microscopy (SEM) to explore the influence of seed coat on the germination of seeds of tallow tree (Sapium sebiferum (Linn) Roxb.). Tallow tree seeds had good water permeability. We found that germination of cabbage seeds was inhibited when cabbage seeds were soaked in extracted solutions from tallow tree seed coat. Seed coat structure at the side of the radicle appeared to be a barrier to seed germination. We tested methods to break tallow tree seed dormancy. Dormancy of tallow tree seeds was overcome by soaking the seeds in 500 mg·L-1 or 1000 mg·L-1 GA3, followed by 100 days of cold stratification. 相似文献