共查询到13条相似文献，搜索用时 281 毫秒
1.
《江苏农业学报》2017,(3)
为了探讨孕酮通过环氧合酶-2(COX-2)/前列腺素2α(PGF2α)信号通路调节家畜黄体溶解的作用机制,本研究采用孕酮受体的特异抑制剂Onapristone处理体外培养的绵羊子宫内膜组织,检测分泌到培养液中的PGF2α浓度以及子宫内膜中PGF2α合成过程中关键酶基因COX-2的mRNA和蛋白质表达水平。结果显示,Onapristone添加到子宫内膜组织培养液中后,内膜组织合成分泌的PGF2α浓度明显下降,且抑制了COX-2 mRNA和蛋白质表达水平,高剂量的Onapristone(20.0μmol/L)处理72 h后COX-2的蛋白表达水平仅为对照组的23%。表明,孕酮通过其受体介导的COX-2/PGF2α信号通路调节家畜的黄体溶解过程,且COX-2对于PGF2α的合成起到重要的作用。 相似文献
2.
用放射免疫分析法,对9头持久黄体病牛中的7头子宫内灌注15甲PGF_2α2～8 mg/头(另2头不用药作对照)和8头卵巢静止病牛中的5头肌注LRH-I 400～600μg/头(另3头不用药作对照)的监测结果表明,持久黄体病牛血浆孕酮浓变为5.54±3.18 ng/ml,灌注15甲PGF_2α后1～3 d,血浆孕酮迅速下降,当降至<0.5 ng/ml时,母牛出现发情;卵巢静止病牛血浆17β-雌二醇浓度为55.91±18.29 pg/ml,肌注LRH-I后,17β-雌二醇浓度缓慢下降,1～3 d后回升,7～15 d达到峰值,母牛出现发情。 相似文献
3.
[目的]探究奶牛子宫内膜上皮细胞来源外泌体及其miR-218在减轻大肠杆菌脂多糖诱发免疫反应中的作用.[方法]用不同质量浓度大肠杆菌脂多糖作用于奶牛子宫内膜上皮细胞系24 h后采用细胞计数试剂盒检测细胞活性;酶联免疫吸附测定检测免疫因子IL-1β和TNF-α释放;采用透射电子显微镜和蛋白印记法验证提取外泌体的粒径和标识蛋白CD63表达,荧光定量聚合酶链式反应法检测外泌体中miR-218表达变化;采用激光共聚焦显微镜观察外泌体与奶牛子宫内膜上皮细胞融合;细胞转染mimics 218检测其对大肠杆菌脂多糖诱发奶牛子宫内膜上皮细胞免疫因子释放的影响.[结果]100μg/mL大肠杆菌脂多糖处理奶牛子宫内膜上皮细胞后,IL-1β、TNF-α含量均显著高于对照组(P＜0.05);大肠杆菌脂多糖刺激奶牛子宫内膜上皮细胞来源外泌体中miR-218的表达量极显著低于奶牛子宫内膜上皮细胞来源外泌体中miR-218的表达量(P＜0.01);正常培养奶牛子宫内膜上皮细胞来源外泌体能够与大肠杆菌脂多糖处理的奶牛子宫内膜上皮细胞融合并与NF-KB (p65)蛋白共定位于细胞质中.将奶牛子宫内膜上皮细胞来源外泌体加入到100 μg/mL大肠杆菌脂多糖处理奶牛子宫内膜上皮细胞24 h后,显著抑制了大肠杆菌脂多糖引起的奶牛子宫内膜上皮细胞培养上清中的IL-1β和TNF-a释放(P＜0.05).同样的,转染进奶牛子宫内膜上皮细胞内mimics 218与磷酸化的NF-KB (p65)核外部分共定位并显著抑制了大肠杆菌脂多糖诱发的奶牛子宫内膜上皮细胞中TNF-α释放(P＜0.05),而对IL-1β释放无显著差异.[结论]miR-218能够随着健康子宫内膜上皮细胞分泌外泌体进入到受到大肠杆菌脂多糖刺激奶牛子宫内膜上皮细胞内,并与磷酸化p65核外部分共定位并抑制大肠杆菌脂多糖引起的TNFα的释放. 相似文献
4.
不同BMPRIB基因型小尾寒羊同期发情处理后FSHR和LHR表达量的研究 总被引：10，自引：1，他引：10
【目的】根据小尾寒羊BMPRIB突变位点进行基因分型。研究发情期小尾寒羊BMPRIB基因型（BB、AB和AA）与FSHR和LHR mRNA表达量的关系。【方法】利用半定量PCR技术。【结果】在发情期BB型右侧卵巢FSHR 的mRNA水平（1.14±0.11）显著高于AA（0.44±0.11）和AB（0.36±0.08）型（P<0.01），但左侧卵巢在基因型间无显著性差异；BB型右侧卵巢LHR的mRNA水平（0.42±0.02）也显著高于AA（0.23±0.02）和AB（0.25±0.04）型（P<0.01），左侧卵巢各基因型间无显著性差异。【结论】FSHR和LHR的mRNA的高表达量可能是引起小尾寒羊较高的产羔数的原因之一。 相似文献
5.
Human prolactin: 24-hour pattern with increased release during sleep 总被引：10，自引：0，他引：10
Human prolactin was measured in plasma by radioimmunoassay at 20 minute intervals for a 24-hour period in each of six normal adults, whose sleep-wake cycles were monitored polygraphically. A marked diurnal variation in plasma concentrations was demonstrated, with highest values during sleep; periods of episodic release occurred throughout the 24 hours. 相似文献
6.
Behavioral effects of progesterone associated with rapid modulation of oxytocin receptors 总被引：4，自引：0，他引：4
The ventromedial nuclei of the hypothalamus (VMN) are important for the control of feminine mating behavior, and hormone action within these nuclei has been causally related to behavior. Estradiol induces receptors for oxytocin in the VMN and in the area lateral to these nuclei over the course of 1 to 2 days, and progesterone causes, within 30 minutes of its application, a further increase in receptor binding and an expansion of the area covered by these receptors lateral to the VMN. The rapid progesterone effect appears to be a direct and specific effect of this steroid on the receptor or membrane, because it was produced in vitro as well as in vivo and was not mimicked by a variety of other steroids. The effect of progesterone occurred in the posterior part of the VMN, where oxytocin infusion facilitated feminine mating behavior; it did not take place in the anterior part of the VMN, where oxytocin infusion had no effect on mating behavior. 相似文献
7.
通过对泥鳅进行生殖激素的注射,研究分别注射25、30、35IU/g剂量的hCG、分别注射10、12、14μg/尾剂量的LHRH-A2、分别注射16IU/g＋10μg/尾、18IU/g＋ 10μg/尾、20IU/g＋ 10μg/尾剂量的hCG＋LHRH-A2对泥鳅催产效果的影响.结果表明：单独使用hCG注射量以30IU/g效果最好.单独使用LHRH-A2其注射量以12μg/尾效果最好.复合激素hCG＋LHRH-A2其注射量以18IU/g＋10μg/尾效果最好.综合考虑抗药性以及价格因素后,实际生产中建议使用12μg/尾LHRH-A2为宜. 相似文献
8.
Tyzio R Cossart R Khalilov I Minlebaev M Hübner CA Represa A Ben-Ari Y Khazipov R 《Science (New York, N.Y.)》2006,314(5806):1788-1792
We report a signaling mechanism in rats between mother and fetus aimed at preparing fetal neurons for delivery. In immature neurons, gamma-aminobutyric acid (GABA) is the primary excitatory neurotransmitter. We found that, shortly before delivery, there is a transient reduction in the intracellular chloride concentration and an excitatory-to-inhibitory switch of GABA actions. These events were triggered by oxytocin, an essential maternal hormone for labor. In vivo administration of an oxytocin receptor antagonist before delivery prevented the switch of GABA actions in fetal neurons and aggravated the severity of anoxic episodes. Thus, maternal oxytocin inhibits fetal neurons and increases their resistance to insults during delivery. 相似文献
9.
Yuqin WANG Zhilong TIAN Huibin SHI Qiujue WU Fang YANG Yuanxiao LI Xiaohui ZHANG Jianping WANG Mingxing CHU 《农业科学与技术》2017,18(5)
Hu Sheep is a local sheep breed in the plain of Taihu,China.Due to the natural conditions in Taihu and human domestication,Hu Sheep is gradually featured with high adaptability,fast growth rate,early maturity and high productivity,which make them to be a unique and rare breed of sheep.In the present study,an experiment was conducted by means of instantaneous scan sampling,focal animal sampling and all-occurrence sampling to learn about Hu Sheep's behavior and activity pattern under certain feeding management and ecological condition.The result showed that the activity time budgets for several behaviors were different between rams and ewes,with respect to feeding (rams 30.60％ vs.ewes 33.23％),ruminating (33.43％ vs.35.30％);sleeping (19.92％ vs.13.69％),standing (4.13％ vs.5.26％),moving (8.10％ vs.7.46％),and other behaviors (3.86％ vs.5.06％).A circadian activity rhythm was shown in some behaviors;e.g.,total feeding time was greater in the daytime than at night,whereas resting time showed the opposite tendency.Moving mostly occurred in the daytime,and ruminating mainly occurred at night.Additionally,feeding activity occurred 32.22 times per day in rams and 31.73 times per day in ewes,whereas ruminating activity after feeding occurred 19.83 times per day in rams and 24.34 times per day in ewes.For each food bolus,the average ruminating time was 51.22 s in rams and 49.52 s in ewes,with 68.22 bouts of rumination per day in rams and 57.35 bouts of rumination in ewes.By conducting the experiment,we studied and grasped the local Hu Sheep's main behavior and regularity which are of great significance on improving the feeding management and reducing diseases. 相似文献
10.
Sulfhydryl groups and estradiol-receptor interaction 总被引：5，自引：0，他引：5
The characteristic ability of rat uteri to take up tritiated estradiol in vitro or to retain estradiol previously incorporated either in vivo or in vitro is destroyed by treating the tissue with various sulfhydryl-blocking reagents. The two radioactive estradiol-receptor complexes, observed in uterine homogenates in the supernatant fraction and in an extract of the nuclear fraction, respectively, are disrupted by brief exposure to organic mercurials in the cold. Sulfhydryl groups of uterine receptor substances apparently play a vital role in estradiol binding, perhaps indirectly through contribution to receptor conformation. 相似文献
11.
Estrogen receptor analysis by flow cytometry 总被引：2，自引：0，他引：2
A fluorescently labeled estradiol, N'-fluoresceino-N'-(17 beta-estradiol hemisuccinamide) thiourea (FE) was used for measuring estrogen receptor content per cell in tumor cells. The cellular content of FE was measured quantitatively by flow cytometry. Binding of FE occurs in the nanomolar concentration range, an indication of the high affinity of the labeled estradiol. Competition of FE for binding sites is observed with estrogens, but not with progestins, androgens, or glucocorticosteroids, indicating the specificity of FE binding. In contrast to other estrogen receptor assays, this new technique requires a small sample size (about 5000 cells) and permits the assessment of heterogeneity in estrogen receptor expression among tumor cells. 相似文献
12.
用免疫组织化学方法检测雌二醇(estradiol)及其受体(estrogen receptor)在北方山溪鲵(Batrachuperus tibetanus)精巢发育周期中的表达变化。结果表明,在4~5月,雌二醇及受体在精原细胞的胞质中强表达;6月,雌二醇在精母细胞的胞质中表达较弱,其受体在精母细胞的核质中有较强表达;7月,雌二醇及受体在精子细胞的胞质中表达;8月,雌二醇及受体在精原细胞的胞质中和变态后的精子头部有弱表达;9-11月,两者均在精原细胞的胞质中及精子头部有较强表达。说明雌二醇及其受体存在于整个精巢周期中,二者在生精细胞中的强弱变化基本一致,其变化规律与精子发育周期密切相关;雌激素受体多存在于生精细胞的胞质中,雌激素可能以非基因组效应调控细胞的增殖,并与精子的发育成熟密切相关。 相似文献
13.
A single daily "surge" in pituitary luteinizing hormone release was observed in ovariectomized-estrogen-treated hamsters expressing an intact circadian rhythm of locomotor activity. In contrast, two luteinizing hormone surges occurred within a single 24-hour period in hamsters whose activity rhythm had dissociated or "split" into two distinct components. These observations indicate that both behavioral and endocrine circadian rhythms are regulated by the same multioscillator system, which seems to be composed of at least two distinct circadian oscillators. 相似文献