共查询到18条相似文献，搜索用时 625 毫秒
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为解决奶白菜杂交种生产中的杂交制种手段问题,配制优良杂交种.以核不育“复等位基因遗传”假说为指导,以青梗白菜核基因雄性不育系00S107作不育源,采用连续回交转育同时测交鉴定基因型的方法,定向转育奶白菜核基因雄性不育系.并利用转育成的不育系与奶白菜优良自交系配制杂交组合,进行了杂种优势分析.选育出园艺学性状与目标品系相似,具有100％不育株率和不育度的奶白菜核基因雄性不育系GMS3,筛选出2个产量高、整齐度高的优异杂交组合GMS3×B1、GMS3×B2.定向转育模式兼顾了不育基因和园艺学性状的转育,解决了奶白菜核不育系转育和利用的难题. 相似文献
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利用分子标记辅助选育大白菜核基因雄性不育系 总被引：3，自引：0，他引：3
本研究以含不育基因Ms的大白菜细胞核复等位基因型雄性不育两用系"AB01"的可育株(MsfMs)为供体亲本,以高代自交系‘a20’(msms)为轮回亲本,采用杂交和连续回交转育方法,利用与不育基因Ms连锁的SCAR标记syau_scr01辅助不育基因Ms选择,成功地将不育基因转育到可育品系‘a20’中,育成了不育度和不育株率均为100%,植物学性状与自交系‘a20’相近的新核不育系GMS4。选择结果表明syau_scr01选择的准确率为100%,验证了该标记可以用于大白菜核不育系转育辅助选择。 相似文献
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大白菜细胞核复等位基因型雄性不育系转育研究 总被引：2，自引：0，他引：2
为解决具有100%不育株率的大白菜细胞核复等位基因型雄性不育系转育难的问题,根据该不育系的遗传特性,设计了不育系的转育方案。采用常规的有性杂交、回交和自交方法,将青帮核不育系3A的不育基因转入白帮自交系C34中,育成了新的核基因雄性不育系A4。植物学性状鉴定结果表明,新育成的核不育系A4的株高、株幅、叶长、叶宽、叶柄长、叶球高、叶球粗、叶球毛重和叶球净重等的平均值和变异系数均接近于C34。 相似文献
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为解决白菜薹杂种优势利用中的杂交制种手段问题,以大白菜复等位基因型雄性不育系为不育源,设计定向转育方案,采用连续回交的方法转育不育性和农艺性状,同时利用分子标记辅助选择目标基因型植株,向白菜薹自交系中转育不育基因,创制白菜薹雄性不育系。通过26对SSR引物的筛选鉴定,获得与显性雄性不育基因Ms紧密连锁、且同样可以标记同一位点恢复基因Msf和可育基因ms的分子标记GSSR1。经过3个世代的回交转育,创制出了具有100%不育度和100%不育株率的白菜薹复等位基因型雄性不育系BGMS-3。以BGMS-3为母本,与6个白菜薹自交系杂交,筛选出1个强优势组合C3。 相似文献
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不同来源的大白菜核不育两用系存在不同的遗传机制,其不育株与可育株都各略占50％并存。在利用上,50％不育株可作母本用于配制F_1杂种和核基因互作雄性不育系;50％可育株可用作F_1杂种父本、质核复合雄性不育保持系和核基因互作雄性不育临保系,同时自交还可快速选育自交系。在优势育种上,大白菜核不育两用系具有一定开发潜力和应用前景。 相似文献
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利用杂交种转育大白菜核基因雄性不育系的研究 总被引：2，自引：0，他引：2
利用杂交种绿星58的不育株作不育源,以大白菜核基因雄性不育复等位基因遗传假说为依据,转育新的核基因雄性不育系,以解决有些育种单位获得不育源难及具有100%不育株率的大白菜核基因型雄性不育系转育难的问题。采用常规有性杂交、回交和自交方法,育成了含有待转育亲本C1-4遗传基因的雄性不育系A2及其相应的甲型两用系AB2和临时保持系B2,并对它们及亲本C1-4和绿星58进行农艺性状鉴定和病害调查。结果表明,新育成的核不育系、两用系和临时保持系的株高、株幅、叶长、叶宽、叶柄长、叶球高和叶球粗等的平均值和变异系数以及病情指数均接近于C1-4,而叶球毛重和叶球净重等的平均值和变异系数均接近于绿星58。 相似文献
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白菜型油菜(B. campestris L.)双显性核不育896AB的选育 总被引：9，自引：1，他引：8
以北方白菜型油菜自交系Ｓ３－１８为母本,加拿大白菜型春油菜Ｔｏｂｉｎ为父本杂交,在自交２代中出现不育株３７ＡＢ,经研究认为,该不育材料是由２种不育基因（ＭＳＭＳｒｆｒｆ,ＭＳｍｓｒｆｒｆ）,４种保持基因（ＭＳＭＳＲｆｒｆ,ＭＳｍｒＲｆｒｆ,ｍｓｍｓＲｆｒｆ,ｍｓｍｓｒｆｒｆ）组成的杂合不育群体,用成对保持,双隐性测交的方法,经２～３个世代的选育,育成８９６ＡＢ双显性不育系及１２５ａｂ等双隐性临保系 相似文献
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Wnafstu棉花雄性不育系遗传类型研究 总被引：3，自引：0，他引：3
以中棉所41、陕7359（系）、陕2089（系）、陕2234、陕024（系）5个陆地棉种质和8046-1、8046-2、8046-3三个与不育系具相同遗传背景的可育种质为测试父本,采用连续多代和多父本分别株对株测交,对西北农林科技大学发现的暂定名Wnafstu的雄性不育系进行研究。结果表明,5个陆地棉种质父本与不育系测交后,子代均为100%不育株;3个与不育株具相同遗传背景的可育株分别与不育株测交后,子代有不育和可育株两种类型,分离没有规律。据此认为,该不育系属细胞质雄性不育类型。一般陆地棉种质与其杂交,都可保持其不育性。与其具相同遗传背景的可育种质对不育系育性有恢复功能。提出了对该不育系下一步研究的方向。 相似文献
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Five restorers of ‘polima’ cytoplasmic male sterility (pol CMS) cannot restore the fertility in dominant genie male sterility (DGMS). A dominant male sterility gene from both, a DGMS line Rs l046AB and DGMS hybrid ‘Zhongza No. 3’, was successfully introduced into Polima cytoplasm. A random-mating population of pol CMS restorers was established by using many double-low pol CMS restorers as pollinators to cross continuously to the DGMS plants which had Polima cytoplasm. 相似文献
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A male sterile line with dominant gene (Ms) in cabbage (Brassica oleracea var. capitata) and its utilization for hybrid seed production 总被引：4，自引：0，他引：4
Zhiyuan Fang Peitian Sun Yumei Liu Limei Yang Xiaowu Wang Anfu Hou Chunsong Bian 《Euphytica》1997,97(3):265-268
A male sterile plant, 79-399-3, was identified from a spring cabbage line 79-399 in 1979. Light-microscopic studies with paraffin
section indicated that meiotic division stopped at the tetrad stage. The ratio of male sterile plants to fertile plants in
the progenies of test crosses was 1:1 in five experiments conducted in 1982 and 1991–1994. However, some male sterile plants
were sensitive and developed a very low number of viable pollen grains. When sensitive male sterile plants were selfed, the
progenies segregated into male sterile and fertile plants at a ratio of 3:1. The male sterile plants from selfing of sensitive
male sterile plants were propagated by tissue culture and crossed with different inbred lines. From the progenies of the crosses,
populations with 100% male sterile plants were observed. The results indicated that homozygous dominant male sterile (MsMs)
plants segregated from the selfed progenies. Populations with 100% male sterile plants and stable male sterility were developed
as male sterile lines. Several favorable combinations were also selected by crossing the male sterile lines with inbred lines
with high combining ability for desirable horticultural characters.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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To produce hybrid seeds of Wutacai (Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee), a “directional transfer program” was designed to breed the multiple-allele male sterile line of Wutacai. A multiple-allele male sterile line of Naibaicai (Brassica campestris L. ssp. chinensis L., S01) was used as the male sterile resource, and an inbred line of Wutacai (WT01) was used as the target line. Recurrent backcrossing was employed to transfer the male sterility and other botanical traits simultaneously, while the genotype was identified through test crossing. The male sterility was successfully transferred from S01 to WT01. A new male sterile line, GMS-3, with similar botanical traits to WT01, was bred. Four hybrid combinations were generated with GMS-3 as the female parent. One hybrid (C1) that contained the most desirable traits was developed from the new male sterile line. 相似文献
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The male sterility (ms) of three recessive genie ms lines, 117AB, S45AB and 9012AB, was controlled in each case by two recessive male sterility genes. The genes responsible in 117AB and S45AB are the same, but they are genetically different from those in 9012AB. 相似文献
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抑制消减杂交法研究复等位基因遗传的 总被引：1，自引：1，他引：0
AB01是本课题组培育的复等位基因遗传的核雄性不育大白菜甲型“两用系”,目前已建立了一套该材料的应用技术体系,但其不育分子机制尚不明确。本研究以AB01的不育株和可育株为材料,利用抑制差减杂交技术构建了正反抑制差减cDNA文库,并通过测序及生物信息学手段寻找育性相关基因,以此来推断该材料的不育分子机制。研究中共找到27个差异表达基因,其中25个基因在NCBI数据库中均有同源序列,这些基因中7个与花发育相关,5个与脂类代谢相关,3个与活性氧及能量代谢相关,3个与光合作用及叶绿体合成相关,其余7个为功能未知基因。由此推测复等位基因遗传的核雄性不育大白菜不育的发生与脂类、能量代谢及光合作用有关。 相似文献