共查询到20条相似文献，搜索用时 15 毫秒
1.
鸡胚破骨细胞的分离培养与鉴定 总被引：5，自引：0，他引：5
从18日龄鸡胚长骨（股骨和胫骨）中机械分离破骨细胞于盖玻片和骨片上培养,以建立鸡胚破骨细胞体外培养方法。结合倒置相差显微镜、抗酒石酸酸性磷酸酶（TRAP）染色、甲苯胺蓝染色及透射电镜（TEM）和扫描电镜（SEM）对其形态和功能进行观察鉴定。结果表明,直接分离的细胞具有典型的破骨细胞的形态特点：属多核大细胞,TRAP染色阳性,能在骨片表面形成吸收陷窝。结论：鸡胚长骨机械分离法可获得一定数量并具有骨吸收活性的破骨细胞。 相似文献
2.
鸡胚肺动脉内皮细胞与平滑肌细胞的分离培养及鉴定 总被引：1，自引：0，他引：1
探索简单快速实用的鸡胚肺动脉内皮细胞和平滑肌细胞培养方法:在严格无菌条件下对肺动脉内皮细胞和平滑肌细胞分离培养,待细胞融合度达约80%时进行传代,得到的内皮细胞利用差速消化法纯化,并观察2种细胞形态特征及培养特性,待细胞稳定生长后,采用荧光免疫组化技术鉴定细胞,并进行冻存和复苏,观察复苏后细胞活性。结果显示:24 h后组织块周围即有少量细胞爬出,内皮细胞大量生长后呈突起状排列,立体感强,平滑肌细胞在培养后的第5天呈现"峰谷"样外观,经免疫荧光法证明是内皮细胞和平滑肌细胞的纯培养,冻存于液氮中1~2个月复苏后细胞的活性都较好,可以恢复旺盛生长。结论:本研究提出的组织贴块法能成功获得内皮细胞和平滑肌细胞的纯培养,比酶消化法操作简单,更适合小血管内皮细胞、平滑肌细胞的原代培养。 相似文献
3.
Treatment of normal chick embryo fibroblasts with phorbol myristate acetate causes those cells to express many of the phenotypic properties of virally transformed cells and also enhances the expression of transformed properties in Rous sarcoma virus-transformed chick embryo fibroblasts. We report here that phorbol myristate acetate has little or no effect on the level of protein kinases encoded by the viral src or endogenous sarc genes. 相似文献
4.
将引进的新城疫V4无毒耐热毒株连续用鸡胚传代.每次挑选48～96 h有明显病变的死亡鸡胚,取其尿囊液毒于-20℃保存,观察V4毒株对鸡胚毒力的病理变化.结果显示,经过20代次的鸡胚毒力筛选.V4毒株对鸡胚的致死率从小于10%上升至20%以上,接种后120 h的活胚有明显地萎缩症状.将鸡胚20代次以上筛选的V4病毒液在鸡胚成纤维细胞上传代培养,并进行蚀斑筛选纯化病毒,直到第5代才出现明显地细胞病变.使用二层琼脂进行病毒的蚀斑分析,第二层琼脂糖在第一层凝固后72 h覆盖,8 h后NDV鸡胚成纤维细胞适应株的蚀斑出现.蚀斑大小直径在1～2 mm变化.通过对NDV V4鸡胚毒力筛选和蚀斑纯化.获得了一株蚀斑克隆株(NDV HB92). 相似文献
5.
1-Adamantanamine hydrochloride: inhibition of Rous and Esh Sarcoma viruses in cell culture 总被引：5，自引：0，他引：5
I-Adamantanamine hydrochloride, added to chick embryo cells, inhibited focus production upon subsequent inoculation of the cells with Rous and Esh sarcoma viruses. Addition of the chemical to suspensions of Rous and Esh viruses before they were added to chick cell cultures did not inactivate the viruses; and the addition to chick cells did not prevent plaque formation following their inoculation with Newcastle disease virus. This indicates that cells treated with 1-adamantanamine hydrochloride are capable of supporting viral replication, and these observations suggest that the drug inhibits growth of two oncogenic viruses by prevention of virus penetration, but not by a virucidal effect or cell intoxication. 相似文献
6.
Homologous viral interference: induction by RNA from defective particles of vesicular stomatitis virus 总被引：2，自引：0，他引：2
T Sreevalsan 《Science (New York, N.Y.)》1970,169(949):991-993
The viral RNA isolated from the defective particles of vesicular stomatitis virus was capable of interfering with the replication of this virus in chick embryo cells. The data indicate that the interfering ability of the defective particles of this virus is due to their nucleic acid component. 相似文献
7.
Protection tests in guinea pigs indicate that vaccines prepared from virus propagated in chick embryo cell cultures are as effective as the purified whole chick embryo vaccines which are currently used for human immunization against eastern equine encephalomyelitis. 相似文献
8.
B P Toole 《Science (New York, N.Y.)》1973,180(83):302-303
The formation of cartilage-like aggregates in high-density stationary cultures of trypsinized chick embryo precartilage cells is blocked by low concentrations of hyaluronate. Thyroxine (and triiodothyronine), growth hormone, calcitonin, and adenosine 3',5'-monophosphate prevented this inhibition by hyaluronate, whereas other hormones tested did not. 相似文献
9.
Secretory activity and oncogenicity of a cell line (MDCK) derived from canine kidney 总被引：26，自引：0，他引：26
A cell line (MDCK) of dog kidney origin grows on a glass surface as a mosaic of epithelium with many multicellular hemispherical vesicles. The cells lining the blisters actively secrete into the cyst cavities. Suspensions of these cells injected intravenously in the chick embryo produce brain metastases resembling adenocarcinoma. 相似文献
10.
When the lens of the 5-day chick embryo is surgically reversed so that its epithelium faces the neural retina the elongation of those lens cells which have already differentiated is arrested and the epithelial cells differentiate into a new set of lens fibers. This internal reorganization, together with a reversal in polarity at the lens equator, results in a complete reversal of the polarity of the-entire lens. 相似文献
11.
12.
Raf kinases have been linked to endothelial cell survival. Here, we show that basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) differentially activate Raf, resulting in protection from distinct pathways of apoptosis in human endothelial cells and chick embryo vasculature. bFGF activated Raf-1 via p21-activated protein kinase-1 (PAK-1) phosphorylation of serines 338 and 339, resulting in Raf-1 mitochondrial translocation and endothelial cell protection from the intrinsic pathway of apoptosis, independent of the mitogen-activated protein kinase kinase-1 (MEK1). In contrast, VEGF activated Raf-1 via Src kinase, leading to phosphorylation of tyrosines 340 and 341 and MEK1-dependent protection from extrinsic-mediated apoptosis. These findings implicate Raf-1 as a pivotal regulator of endothelial cell survival during angiogenesis. 相似文献
13.
A subcellular infectious material has been found in a strain of the amoeba Naegleria gruberi, strain EG, which is capable of infecting chick embryo cells and causing them to undergo cytopathic changes with the release of more infectious material. The material is present in two lines of the amoeba which were separated shortly after the isolation of the strain and subsequently maintained in separate laboratories. 相似文献
14.
An endonucleolytic activity has been identified in nuclear extracts of chick embryo bursa and mouse fetal liver cells. The activity introduces a double-strand cut in the vicinity of the recombination site of immunoglobulin joining gene segments. The cleavage occurs at the dinucleotide pair TG-AC. This activity is a good candidate for the putative endonuclease involved in recombination of the immunoglobulin variable, diversity, and joining regions. It is distinct from the endonuclease activities previously reported by others. 相似文献
15.
When cultures of cells derived from the entoderm of the chick embryo were infected with trachoma virus, cytoplasmic inclusion bodies composed of viral particles were easily demonstrated. The inclusions are similar to those found in stained smears from trachomatous eyes and they developed in a sequence characteristic for this group of viruses. This method of culture appears to offer a valuable additional tool for study of the trachoma agent. 相似文献
16.
本实验将鸡胚采用冻干和熟制的方法分别制作出熟制鸡胚胚体混悬液(CCES)和生制鸡胚胚体混悬液(FCES),灌喂小鼠30d。通过测定小鼠脾脏指数,血清溶血素活性,巨噬细胞吞噬率、吞噬指数以及小鼠力竭至死游泳时间分析,探索进食鸡胚对小鼠免疫功能和抗疲劳能力的影响。结果发现FCES、CCES均能提高小鼠脾脏指数,提高血清HC50值,增强小鼠腹腔巨噬细胞吞噬功能,显著延长小鼠游泳时间。研究表明FCES、CCES均能增强小鼠免疫功能与抗疲劳作用,但程度有所不同,以FCES总体效果更佳。 相似文献
17.
Ethanol neurotoxicity: effects on neurite formation and neurotrophic factor production in vitro 总被引：3，自引：0，他引：3
The effects of ethanol on chick embryo sensory and spinal cord neurons growing on one of several biological substrates (poly-D-lysine, laminin, or neuron-produced neurite-promoting materials) were examined. Ethanol inhibited process formation by the neurons in a dose-dependent manner and inhibited the production of neurotrophic factors. Neuronal attachment to the substrates, survival of attached neurons, and receptor interactions of sensory neurons with nerve growth factor were not influenced by ethanol. It appears that ethanol alters certain metabolic characteristics of developing neurons. 相似文献
18.
通过对GenBank发表的鸭副粘病毒(DPV)和鸭H9亚型禽流感病毒(AIV,H9)基因序列分析,针对保守区分别设计并合成2对特异性引物.通过对扩增条件的优化,建立了检测两种病毒的快速双重PCR方法.结果表明,该检测方法可同时扩增出DPV (363 bp)和H9亚型AIV (732 bp)的特异性片段,而对照DHV、I... 相似文献
19.
Hypophyseal control of genetic expression during chick feather and skin differentiation 总被引：1，自引：0，他引：1
M B Yatvin 《Science (New York, N.Y.)》1966,153(732):184-185
The normal sequence of molecular events which occurs during feather and skin differentiation in the chick embryo is interrupted by pituitary gland ablation. The characteristic pattern of development is reestablished in hypophysectomized embryos treated with pituitary extract and in hypophysectomized embryos in parabiotic union with their twins within a double-yolked egg. These results suggest that genetic expression in differentiating chick feather and skin after day 12 of incubation is regulated by hormones. 相似文献
20.
Cell aggregation: role of acid mucopolysaccharides 总被引：18，自引：0，他引：18
Factors that induce cell aggregation are released by several types of chick embryo and mammalian cell cultures. These aggregation factors are also present in some serums. The factors in each of the preparations tested were inactivated by treatment with bovine testicular hyaluronidase. Conversely, hyaluronic acid promoted aggregation of only those cells that were aggregated by media containing the factors. These factors appear to be acid mucopolysaccharides, with hyaluronic acid being a major component. 相似文献