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1.
Quantitative trait loci for agronomic traits in soybean   总被引：2，自引：0，他引：2  
There continues to be improvement in seed yields of soybean by conventional breeding, but molecular techniques may provide faster genetic gains. The objective of this study was to identify quantitative trait loci (QTL) associated with the agronomic traits seed yield, lodging, plant height, seed filling period and plant maturity in soybean. To achieve this objective, 101 F6‐derived recombinant inbred lines (RIL) from a population developed from a cross of N87‐984‐16 × TN93‐99 were used. Experiments were conducted in six environments during 2002–2003. Heritability estimates on an entry mean basis from data combined across environments ranged from 0.12 to 0.65 for seed yield and seed filling period, respectively. Composite interval mapping detected one QTL for yield (near Satt076), two for lodging (near Satt225 and Satt593) and four for maturity (near Satt263, Satt292, Satt293 and Satt591) in this population. Additional environmentally sensitive QTL for these traits, and for seed filling period and plant height are also reported. The QTL associated with agronomic traits that we report and the recently released germplasm (PI 636460) from this population may be useful in soybean breeding programmes.  相似文献   

2.
Four forage maize stover quality traits were analysed including in vitro digestibility of organic matter (IVDOM), neutral detergent fibre (NDF), water‐soluble carbohydrates (WSC) and digestibility of NDF (DNDF). We mapped quantitative trait loci (QTL) in three DH (doubled haploid) populations (totally 250–720 DH lines): one RIL population (358 lines) and two testcross (TC) populations, based on field phenotyping at multiple locations and years for each. High phenotypic and genotypic correlations were found for all traits and significant (p < .01) at two locations, and NDF was negatively correlated with the other traits. QTL analyses were conducted by composite interval mapping. A total of 33, 23, 32 and 25 QTL were identified for IVDOM, NDF, WSC and DNDF, respectively, with three, four, five and two major QTL for each. Few consistent QTL for IVDOM, WSC and DNDF were detected in more than two populations. This study contributed to the identification of key QTL associated with forage maize digestibility traits and is beneficial for marker‐assisted breeding and fine mapping of candidate genes associated with forage maize quality.  相似文献   

3.
Yield QTL affected by heading date in Mediterranean grown barley   总被引：1，自引：0，他引：1  
Knowledge regarding quantitative trait loci (QTL) has led to remarkable advances in breeding for a variety of traits, some of which have an effect on yield under particular environmental conditions. However, the same yield QTL are not usually found, even in the same population tested in different environments, as a result of large genotype-by-environment interactions. In this study, we aimed at identifying yield QTL in a series of experiments carried out in Spain. We used a barley doubled haploid population derived from a spring by winter cross. The relationship between heading date and yield, and between the principal heading date QTL and yield, changed depending on the environment. Allelic combinations causing early or late heading dates usually did not favour high yield, whereas intermediate heading dates were associated with not only higher variability but also higher yield potential. To identify QTL for grain yield independent of heading date, the population was divided into three classes: Early , Intermediate and Late . We found three new QTL affecting yield only at specific maturity groups. The implications of these findings in relation to barley breeding are discussed.  相似文献   

4.
The objective of this study was to examine the relationship between heterosis and genetic distance in upland cotton (Gossypium hirsutum L.). Heterosis in the different environments was evaluated and the relationship between heterosis and genetic distance (GD) was determined based on SSR markers for yield quantitative trait loci (QTL). Yields of seed cotton and lint showed a linear relationship with mid‐parent heterosis (MPH) and better‐parent heterosis (BPH). The variation in heterosis for other traits and their correlation with GD may be due to environmental factors or the effort of QTLs tested in the present study may vary in different environments. The present findings provide a foundation for heterotic grouping of parental lines and breeding of new cotton hybrids with improved seed cotton yield. This study calls for more research with stable QTLs as well as advance molecular marker techniques may be used in predicting yield heterosis in a more precise and reliable manner.  相似文献   

5.
Lolium perenne L. (perennial ryegrass) is the principle forage grass species in temperate agriculture. Improving biomass yield still remains one of the most important aims of current forage breeding programmes. A quantitative trait locus (QTL) study investigating biomass yield traits in perennial ryegrass was carried out in greenhouse and field environments. The study is based on an F2 population consisting of 360 individuals derived from two inbred grandparents where the F1 has a large biomass yield phenotype. For both experimental environments co-localized QTL for biomass yield traits including fresh and dry weight and dry matter were identified on linkage groups 2, 3 and 7. A major QTL for fresh and dry weight was identified on LG 3 which explained around 30% of the phenotypic variance in the field experiment. The findings of this study are discussed with regard for their potential in research and breeding.  相似文献   

6.
The construction of genetic maps is an expensive and time-consuming process. The breeder is therefore interested in using maps developed from other mapping populations but this is only possible if the genetic structure is similar for the chromosomal regions of interest. In this paper, maps of three populations of sugar beet (Beta vulgaris L.) with common polymorphic marker loci are compared. Maps were constructed with MAPMAKER 3.0 and JOINMAP 2.0. Both mapping programs gave, in general, the same order for common markers. However, the number of common markers was too low to construct a combined map for all chromosomes. For one population, in contrast to the other two, the map constructed with MAPMAKER 3.0 was much longer than that constructed with JOINMAP 2.0. For two of these populations yield traits were also available from different environments. For quantitative trait loci (QTL) analysis of the yield data, the packages MAPMAKER/QTL 1.1 and PLABQTL were used. No QTL common for the two populations could be detected. The program and the version used strongly influenced the estimated positions of QTLs. There was also a strong interaction with environments.  相似文献   

7.
Four agronomic traits were analysed including dry matter concentration (DMC) and dry matter yield (DMY) for stover, plant height (PHT) and days from planting to silking (DPS). We mapped quantitative trait loci (QTL) in three populations with doubled haploid lines (DHL), one RIL population and two testcross (TC) populations derived from crosses between two of the four populations mentioned above to elite tester lines, based on field phenotyping at multiple locations and years for each; 146–168 SSRs were used for genotyping of the four mapping populations. Significant high phenotypic and genotypic correlations were found for all traits at two locations, while DMC was negatively correlated with the other traits. A total of 42, 41, 54, and 45 QTL were identified for DMC, DMY, PHT, and DPS, respectively, with 9, 7, 12, and 7 major QTL for each trait. Most detected QTL displayed significant interactions with environment. Major QTL detected in more than two populations will contribute to marker‐assisted breeding and also to fine mapping candidate genes associated with maize agronomic traits.  相似文献   

8.
Heterosis is a phenomenon whereby hybrids of inbred lines produce favourable phenotypes that exceed those of their parents. Traits of interest are higher yield and stronger stress tolerance. The two‐line super‐hybrid rice ‘Liangyoupei9’ (LYP9) shows superiority to both its elite inbred line ‘93‐11’ and ‘Pei'ai64s’ (‘PA64s’) parents and conventional hybrids. However, the genetic basis of its hybrid vigour, especially yield determination, remains elusive. In the present study, a set of 156 chromosome segment substitution lines (CSSLs) carrying overlapping segments from ‘PA64s’ in a genetic background of ‘93‐11’ were constructed and planted in six environments. Three major agronomic traits, viz. panicle length (PL), heading date (HD) and plant height (PH), and five yield‐related traits, viz. grain weight per panicle (GWP), number of grains per panicle (GPP), 1000‐grain weight (TGW), seed set (SS) and number of panicles of per plant (PPP), were evaluated over 3 years. Quantitative trait loci (QTL) analysis was conducted using a likelihood ratio test based on stepwise regression. Forty‐six putative QTL distributed on 11 chromosomes were detected in more than one year. Remarkably, GWP of four CSSLs carrying positive yield QTL outperformed the recurrent parent ‘93‐11’ by more than 15%, in at least two environments. These results indicate that CSSLs are effective in identifying yield‐associated traits, and lines harbouring such QTL will be rich in resources for future molecular breeding programmes.  相似文献   

9.
X. Yin    S. D. Chasalow    P. Stam    M. J. Kropff    C. J. Dourleijn    I. Bos  P. S. Bindraban 《Plant Breeding》2002,121(4):314-319
Genes contributing to the quantitative variation of a complex crop trait can be numerous. However, using existing approaches, the number of quantitative trait loci (QTL) detected for a trait is limited. Therefore, rather than looking for QTL for a complex trait itself, determining QTL for underlying component traits might give more information. In this study the potential of component analysis in QTL mapping of complex traits was examined using grain yield in spring barley as an example. Grain yield was divided into three components: number of spikes/m2, number of kernels/spike, and 1000‐kernel weight. These traits were measured for individuals of a recombinant inbred‐line population in field trials conducted over 2 years. By the use of an approximate multiple QTL model, one to eight QTL were detected for each trait in a year. Some QTL were mapped to similar positions in both years. Almost all QTL for yield were found at the position of or in close proximity to QTL for its component traits. A number of QTL for component traits were not detected when yield itself was subjected to QTL analysis. However, relative to the QTL for yield itself, all component‐trait QTL did not explain the variation in yield better. The results in relation to the potential of using component analysis in studying complex crop traits are discussed.  相似文献   

10.
To manipulate the composition of the maize kernel to meet future needs, an understanding of the molecular regulation of kernel quality‐related traits is required. In this study, the quantitative trait loci (QTL) for the concentrations of grain protein, starch and oil were identified using three sets of RIL populations in three environments. The genetic maps and the initial QTL were integrated using meta‐analyses. A total of 38 QTL were identified, including 15 in population 1, 12 in population 2 and 11 in population 3. The individual effects ranged from 2.87% to 13.11% of the phenotypic variation, with seven QTL each contributing over 10%. One common QTL was found for the concentrations of grain protein and starch in bin 3.09 in the three environments and the three RIL populations. Of the 38 initial QTL, 22 were integrated into eight mQTL by meta‐analysis. mQTL3 and mQTL8 of the key mQTL in which the initial QTL displayed R2 > 10% included six and three initial QTL for grain protein and starch concentrations from two or three populations, respectively. These results will provide useful information for marker‐assisted selection to improve the quality of the maize kernel.  相似文献   

11.
A doubled haploid (DH) population of 125lines derived from IR64 × Azucena, an indica – japonica cross were grown in three different locations in India during the wet season of 1995. The parents of mapping population had diverse phenotypic values for the eleven traits observed. The DH lines exhibited considerable amount of variation for all the traits. Transgressive segregants were observed. Interval analysis with threshold LOD > 3.00 detected a total of thirty four quantitative trait loci (QTL) for eleven traits across three locations. The maximum number of twenty QTL were detected at Punjab location of North India. A total of seven QTL were identified for panicle length followed by six QTL for plant height. Eight QTL were identified on three chromosomes which were common across locations. A maximum of seven QTL were identified for panicle length with the peak LOD score of 6.01 and variance of 26.80%. The major QTL for plant height was located on Chromosome 1 with peak LOD score of 16.06 flanked by RZ730-RZ801 markers. Plant height had the maximum number of common QTL across environment at the same marker interval. One QTL was identified for grain yield per plant and four QTL for thousand grain weight. Clustering of QTL for different traits at the same marker intervals was observed for plant height, panicle exsertion, panicle number, panicle length and biomass production. This suggests that pleiotropism and or tight linkage of different traits could be the plausible reason for the congruence of several QTL. Common QTL identified across locations and environment provide an excellent opportunity for selecting stable chromosomal regions contributing to yield and yield components to develop QTL introgressed lines that can be deployed in rice breeding program. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

12.
Grain yield is one of the most important and complex quantitative traits in maize breeding. In the present study, a total of 11 connected RIL populations, derived from crosses between elite inbreed “Huangzaosi” as the common parent and 11 elite inbreeds, were evaluated for five yield components and kernel-related traits under six environments. Quantitative trait loci (QTL) were detected for the traits under each environment and in joint analysis across all environments for each population. A total of 146 major QTL with R2 > 10 % in at least one environment and also detected based on joint analysis across all environments were identified in the 11 populations. Lqkwei4 conferring kernel weight and Lqklen4-1 conferring kernel length both located in the adjacent marker intervals in bin 4.05 were stably expressed in four environments and in joint analysis across six environments, with the largest R2 over 27 and 24 % in a single environment, respectively. Moreover, all major QTL detected in the 11 populations were aligned on the IBM2 2008 neighbors reference map. Totally 16 common QTL (CQTL) were detected. Seven important CQTL (CQTL1-2, CQTL1-3, CQTL4-1, CQTL4-2, CQTL4-3, CQTL4-4, and CQTL6-1) were located in bin 1.07, 1.10, 4.03, 4.05, 4.08, 4.09 and 6.01–6.02, respectively. These chromosomal regions could be targets for fine mapping and marker-assisted selection.  相似文献   

13.
Despite the well-recognized importance of grain yield in high-oil maize (Zea mays L.) breeding and production, few studies have reported the application of QTL mapping of such traits. An inbred line of high-oil maize designated ‘GY220’ was crossed with two dent maize inbred lines to generate two connected F2:3 populations with 284 and 265 F2:3 families. Our main objective was to evaluate the influence of genetic background on QTL detection of grain yield traits through comparisons between the F2:3 populations. The field experiments were conducted during the spring in Luoyang and summer in Xuchang, Henan, China. Two genetic linkage maps were constructed with a genetic distance of 2111.7 and 2298.5 cM using 185 and 173 polymorphic SSR markers, respectively. In total, 18 and 15 QTL were detected for six grain yield traits in the two populations. Only one common QTL marker was shared between the two populations. A QTL cluster associated with five traits was identified at bin 1.05–1.06, including the shared QTL for 100GW, which demonstrated the largest effect (16.7%). Among the detected QTL, 12 digenic interactions were identified. Our results reflect the substantial influence of dent maize genetic background on QTL detection of grain yield traits.  相似文献   

14.
越冬栽培稻是一类能越过自然冷冬季节并在第2年春季萌芽、正常开花结实、收获稻谷的水稻品种。本文通过对越冬栽培稻产量性状QTL分析,明确产量相关性状的遗传规律,旨在进一步解析越冬栽培稻产量性状的遗传机制,为育种创新利用提供理论依据。以3份越冬栽培稻构建的3个半同胞F2群体为材料。各考察15个产量相关性状,利用Excel 2003、GraphPad Prism 5.0和QTL IciMapping 4.10软件分析数据、绘制遗传图谱、定位QTL和联合分析。结果表明,产量性状表型值在3群体中呈连续正态分布,表现为数量性状遗传。共检测到37个QTL和26对上位性QTL,贡献率分别介于2.32%～36.31%和1.04%～2.05%;检测到9个同时影响2个及以上产量性状(一因多效)QTL标记区间;以联合分析检测到13个产量性状相关QTL,其中4个QTL区间与单群体检测QTL区间重叠;越冬栽培稻产量相关性状QTL以加–显性效应遗传为主、上位性遗传效应为辅。本研究将为越冬栽培稻产量相关基因挖掘及育种创新利用奠定基础。  相似文献   

15.
Heterosis and mixing effects in barley under drought stress   总被引：1，自引：0，他引：1  
Yield stability is one of the main breeding objectives in breeding for stress environments, such as the semi‐arid areas of Syria. The objectives of this study were to measure the effects of heterogeneity and heterozygosity on yield and yield stability by comparing doubled haploid lines (DHL) in mixed vs. pure stand (influence of heterogeneity) and F2 populations vs. corresponding DHL mixtures (influence of heterozygosity). Six barley lines from two gene pools (LR = landraces, EL = experimental lines) were used to produce nine crosses (two LR × LR, three EL × EL, four LR × EL). The F2 generation and eight DHL per cross were produced from each cross. The six parental DHL, nine F2 populations, nine 8‐line mixtures and 72 DHL in pure stands were tested in five environments under drought stress in north Syria. The mean superiority of F2 populations over DHL mixtures for yield traits across environments and cross combinations ranged between 7.5 and 10%. The effect of heterogeneity was small throughout. For grain yield, harvest index, 1000‐grain weight and plant height significant interactions between heterozygosity levels and environments were observed. The effect of heterozygosity for grain yield increased substantially from ‐1.2% in the highest‐yielding environment to 45.6% in the most stressful environment. Interactions between levels of heterozygosity and cross combinations were significant for most traits. F2 populations were considerably more stable than DHL in pure stands, yet not as stable as DHL mixtures. It is concluded that heterozygosity is more important than heterogeneity in breeding for improved yield and yield stability under drought stress.  相似文献   

16.
A population of 112 F1-derived doubled haploid lines was produced from a reciprocal cross of Brassica juncea. The parents differed for seed quality, seed color and many agronomic traits. A detailed RFLP linkage map of this population, comprising 316 loci, had been constructed, and was used to map quantitative trait loci (QTL) for seed yield and yield components, viz. siliqua length, number of seeds per siliqua, number of siliques per main raceme and 1000-seed weight. Stable and significant QTLs were identified for all these yield components except seed yield. For yield components, a selection index based on combined phenotypic and molecular data (QTL effects) could double up the efficiency of selection compared to the expected genetic advance by phenotypic selection. Selection indices for high seed yield, based on the phenotypic data of yield and yield components, could only improve the efficiency of selection by 4% of the genetic advance that can be expected from direct phenotypic selection for yield alone. Inclusion of molecular data together with the phenotypic data of yield components in the selection indices did not improve the efficiency of selection for higher seed yield. This is probably due to often negative relationships among the yield components. Most of the QTLs for yield components were compensating each other, probably due to linkage, pleiotropy or developmentally induced relationships among them. The breeding strategy for B. juncea and challenges to marker assisted selection are discussed.  相似文献   

17.
Improved cold tolerance during the juvenile phase is a major breeding goal to develop new sorghum cultivars suitable as an alternative energy crop in temperate regions. The objectives of this study were to identify marker‐trait associations for cold tolerance in a sorghum diversity panel fingerprinted with 2620 single nucleotide polymorphism (SNP) markers and to detect quantitative trait loci (QTL) in two F2:3 populations. Traits of interest were dry matter growth rate (DMGR), leaf appearance rate (LAR), chlorophyll content (SPAD) and chlorophyll fluorescence (Fv′/Fm′ and ФPSII) in relation to temperature. The association panel comprised 194 genotypes, while the F2:3 populations consisted of 80 and 92 genotypes. All populations were tested under controlled conditions in a minimum of four temperature regimes ranging from 9.4°C to 20.8°C. QTL were identified for means across environments and regression parameters describing temperature effects. Several marker‐trait associations for mean (m) DMGR, base temperature (Tb) of SPAD and ФPSII and temperature effect on LAR were validated by QTL detected in population 1 or 2. Promising QTL regions were found on chromosomes SBI‐01, SBI‐02, SBI‐03, SBI‐04, SBI‐06 and SBI‐09, among them genomic regions where candidate genes involved in the C‐repeat binding pathway or encoding cold‐shock proteins are located.  相似文献   

18.
Flag leaf-related traits (FLRTs) are determinant traits affecting plant architecture and yield potential in wheat (Triticum aestivum L.). In this study, three related recombinant inbred line (RIL) populations with a common female parent were developed to identify quantitative trait loci (QTL) for flag leaf width (FLW), length (FLL), and area (FLA) in four environments. A total of 31 QTL were detected in four environments. Two QTL for FLL on chromosomes 3B and 4A (QFll-3B and QFll-4A) and one for FLW on chromosome 2A (QFlw-2A) were major stable QTL. Ten QTL clusters (C1–C10) simultaneously controlling FLRTs and yield-related traits (YRTs) were identified. To investigate the genetic relationship between FLRTs and YRTs, correlation analysis was conducted. FLRTs were found to be positively correlated with YRTs especially with kernel weight per spike and kernel number per spike in all the three RIL populations and negatively correlated with spike number per plant. Appropriate flag leaf size could benefit the formation of high yield potential. This study laid a genetic foundation for improving yield potential in wheat molecular breeding programs.  相似文献   

19.
干旱胁迫对玉米产量及其相关性状有重要影响。本研究以我国玉米育种骨干亲本齐319和掖478分别和黄早四组配构建的两个F2:3群体为材料,应用逐步联合分析的QTL定位方法,剖析新疆不同水分环境下(包含水区和旱区)玉米产量构成因子及籽粒相关性状的遗传基础。结果表明,在相同水分处理不同年份间产量构成因子和籽粒相关性状超过70%的QTL可稳定表达,旱区QTL的稳定性明显低于水区,当全部环境联合分析时,各性状QTL稳定性呈现一定程度的降低,但超过60%的QTL仍然稳定表达。两群体中共检测到11个环境钝感的主效QTL(在2个以上环境中检测到,且至少在一个环境下的贡献率大于10%),分布在bin1.10、2.00、4.09、7.02、9.02、10.04和10.07共7个基因组区段上,除bin10.04外所有环境钝感的主效QTL在全部环境下稳定表达。因此,玉米产量构成因子和籽粒相关性状的QTL在新疆相同水分处理不同年份间,甚至不同水分条件下大部分均可稳定表达,这些主效QTL位点可为抗旱分子育种和进一步精细定位提供参考。  相似文献   

20.
Heterosis, or hybrid vigour, has been used to improve seed yield in several important crops for decades and it has potential applications in soybean. The discovery of over‐dominant quantitative trait loci (QTL) underlying yield‐related traits, such as seed weight, will facilitate hybrid soybean breeding via marker‐assisted selection. In this study, F2 and F2 : 3 populations derived from the crosses of ‘Jidou 12’ (Glycine max) × ‘ZYD2738’ (Glycine soja) and ‘Jidou 9’ (G. max) × ‘ZYD2738’ were used to identify over‐dominant QTL associated with seed weight. A total of seven QTL were identified. Among them, qSWT_13_1, mapped on chromosome 13 and linked with Satt114, showed an over‐dominant effect in two populations for two successive generations. This over‐dominant effect was further examined by six subpopulations derived from ‘Jidou12’ × ‘ZYD2738’. The seed weight for heterozygous individuals was 1.1‐ to 1.6‐fold higher than that of homozygous individuals among the six validation populations examined in different locations and years. Therefore, qSWT_13_1 may be a useful locus to improve the yield of hybrid soybean and to understand the molecular mechanism of heterosis in soybean.  相似文献   

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