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1.
To determine if egg drop syndrome 76 virus infection is among the causes of lowered egg productivity in commercial poultry
farms in South Eastern Part of Nigeria and to know the prevalence of the infection, ten farms with history of lowered egg
production in Nsukka local government area of Enugu State were randomly selected. Sera from ten hens in each of the selected
farms were assayed for antibodies against EDS 76 virus by the haemagglutination-inhibition (HI) test. The mean HI titre of
the ten hens in each of the farms was recorded as EDS - 76 antibody titre for the farm. Nine out of the 10 farms tested were
positive for EDS - 76 antibodies with HI titres ranging between 16 and 256. Out of 10 flocks with production of 65% and above
9 were EDS-76 HI negative. 相似文献
2.
对应用当地分离病毒株研制的鸡产蛋下降综合症系列油乳剂灭活苗进行了免疫试验。结果表明,免疫后,鸡产蛋下降综合症（ＥＤＳ７６）油乳剂灭活苗、鸡新城疫（ＮＤ）－产蛋下降综合症二联油乳剂灭活苗及鸡新城疫－鸡传染性支气管炎（ＩＢ）－产蛋下降综合症三联油乳剂灭活苗免疫组的血清ＥＤＳ７６ＨＩ抗体迅速上升,维持４个月后仍在６．８－８（ｌｏｇ２）以上,并且能够抵抗强毒的攻击。证明所研制的ＥＤＳ７６油苗、ＮＤ－ＥＤＳ７６二联油苗、ＮＤ－ＩＢ－ＥＤＳ７６三联油苗对ＥＤＳ７６具有良好的免疫作用,能够抵抗ＥＤＳ７６强毒的攻击并产生高而持久的血清ＨＩ抗体。此外,对ＮＤ－ＥＤＳ７６二联苗、ＮＤ－ＩＢ－ＥＤＳ７６三联苗免疫组的血清ＮＤＨＩ抗体测定结果表明,上述二联苗、三联苗能产生与接种ＮＤ油苗一样良好的ＮＤ免疫效果,在免疫后１３０天时,其血清ＨＩ抗体仍高达９－１１（ｌｏｇ２）以上,与对照组有极显著的差异 相似文献
3.
七彩山鸡传染性法氏囊病及其并发感染 总被引：1，自引：0，他引：1
1998年,贵阳地区两个珍禽养殖场（A场和B场）的七彩山鸡暴发一种以急性死亡、法氏囊肿大为特征的传染病。病料对试验鸡能引起临床症状和死亡,复制出在自然病例中观察到的典型病理变化。A场无并发感染,死亡率为11．4％,死亡高峰出现在第5天;B超由于致病性链球菌的混合感染,死亡率上升到50％,死亡高峰提前到第3天,剖解病变更加复杂化,该场的致病性链球菌是加剧本病高死亡率和严惩的出血性素质的真正原因。该病毒能引起实验鸡出现临床症状和死亡,复制出在自然病例中观察到的曲型病理变化。另对两场的鸡新城疫（ND）和鸡减蛋综合症（EDS）的血清效价测定结果表明,部分鸡群的ND免疫不合格,鸡群曾被EDS病毒感染过。 相似文献
4.
A simple, user-friendly, and rapid method to detect the presence of antibodies to egg drop syndrome 76 (EDS) virus in chicken sera based on an immunofiltration (flow-through) test was developed. Purified EDS virus antigen was coated onto nitrocellulose membranes housed in a plastic module with layers of absorbent filter pads underneath. Following addition of serum to be tested and washing, monoclonal antibodies or polyclonal serum to chicken immunoglobulin G (IgG) was used as a bridge antibody to mediate binding between EDS virus-specific IgG and protein A gold conjugate. The appearance of a pink dot indicated the presence of antibodies to EDS virus in the sample tested. The results could be obtained within 5-10 min. The developed immunofiltration test could detect antibodies in the sera of experimentally vaccinated chickens from 2 wk postvaccination. With field sera samples, this test was positive in samples having hemagglutination inhibition titers of 8 and above. This test has the potential to be used as a field-based kit to assess seroconversion in EDS-vaccinated flocks. 相似文献
5.
使用鸡新城疫病毒LaSota株和产蛋下降综合征病毒分离株HE03株制备鸡新城疫和产蛋下降综合征二联灭活氢氧化铝胶疫苗。把40只16周龄SPF蛋鸡分成A、B 组,分别是30只、10只。A组接种0.5 mL鸡新城疫和产蛋下降综合征二联灭活氢氧化铝胶疫苗,B组作为对照组,每隔14 d采集血清样本直到接种后第6周。至22周龄时,A组再次分成A1和A2两组。A2组与B组的鸡接受强致病性的产蛋下降综合征病毒攻击。结果表明,A组在不同时间采集的血清抗鸡产蛋下降综合征病毒的最高的抗体出现在接种后的第4周,抗鸡产蛋下降综合征病毒的HI抗体滴度从1∶128到1∶512不等,几何平均滴度（GMT）为1∶111;第4周时,抗鸡新城疫病毒的HI抗体滴度GMT为1∶134。该疫苗能保护鸡抵抗强致病性的产蛋下降综合征病毒和鸡新城疫病毒的感染,B组在攻毒感染后产蛋率显著下降,出现畸形蛋、软壳蛋和无壳蛋。 相似文献
6.
分别用三个滴度的ＮＤＶ、ＥＤＳ_７６Ｖ、ＩＢＶ作正交配比，制成八组不同配比的三联油佐剂灭活苗，鸡免疫后每月采血分离血清，应用ＨＩ监测其抗体，结果表明，三种病毒适当配比，不产生干扰，疫苗的免疫效果与抗原量有关。本疫苗与Ｉｎｔeｒｖｃｔ公司生产的ＮＤ－ＩＢ－ＩＢＤ三联油佐剂灭活苗作比较试验，免疫鸡所产生的ＮＤ、ＩＢ抗体无显著性差异。应用含抗原量最低口比的疫苗，对不明原因的有产蛋率下降疫病感染史的鸡群作免疫接种，冬季该场蛋鸡群普遍发生产蛋率明显下降的疫病，用本苗免疫的鸡群产蛋享未受影响。 相似文献
7.
8.
鸡转移因子最低有效浓度的确定 总被引：4，自引：0，他引：4
本文以实验鸡的减蛋综合征血凝抑制抗体效价为指示系统，来确定鸡转移因子的最低有效浓度。首先，用减蛋综合征灭能油佐剂疫苗免疫实验鸡，然后将实验鸡随机分为实验组（共六个组）和对照组。给实验组的实验鸡分别肌肉注射１ｍｌ含不同剂量的高浓度鸡转移因子，对照组注射１ｍｌ生理盐水。随后，定期采血，用微量血凝抑制实验测定其抗体效价的变化。结果表明：实验组有４个组与对照组差异显著（Ｔ〉２．３０６，Ｐ〈０．０５），其它组差异不显著，最低有效浓度为０．０４ｍｌ／只高浓度鸡转移因子。 相似文献
9.
An egg drop syndrome within Australian broiler poultry is described. The syndrome was characterised by delayed onset of laying, a lower peak in egg production and a drop in egg production shortly after reaching peak production. Antibody to virus 127 was detected in 102 of 106 fowl serums tested. Two haemagglutinating viruses were isolated from one affected flock and one was subjected to further study. It was adenovirus-like on electron-microscopic examination and haemagglutination was not inhibited by a specific antiserum to Newcastle disease virus. An antiserum was raised in White Leghorn fowl against the isolate and this antiserum was found to cross-react with virus 127, a prototype virus of Egg Drop Syndrome 76. 相似文献
10.
In order to determine whether the current field strains of egg drop syndrome (EDS) 1976 viruses adapt to chickens, we compared the growth efficiency of three Japanese field strains (PA-1/79, AWI/98, Gifu/01) in chicken and duck embryo liver cells. The growth efficiency in chicken or duck embryo liver cells was almost similar in these strains. The fiber protein may carry the type-specific antigen and the hemagglutination activity, and hexon protein may contain the subgroup-specific antigenic determinants. Therefore, the fiber head and hexon loop 1 DNA domain sequences of the six Japanese field strains UPA-1/79, ME/80, 44/81, Kyoto/91, AWI/98, Gifu/01) were compared, but these DNA domains were identical among the six field strains. Our data suggested that the EDS virus was maintained without discernible changes for the last two decades in the field. 相似文献
11.
鸭黄病毒感染逆转录半套式PCR检测方法的建立 总被引：1，自引：0，他引：1
根据GenBank发布的黄病毒Bagaza毒株的NS3基因的保守序列设计了3条引物,建立了一种适用于鸭黄病毒的半套式PCR快速检测方法。采用该方法对鸭黄病毒山东分离株进行检测,结果半套式PCR对2株分离株均能扩增出277bp的特异性条带,而对鸭瘟病毒、禽流感病毒（H9亚型）、新城疫病毒、传染性法氏囊病病毒、减蛋综合征病毒的扩增结果均为阴性;经检测该方法第1次扩增的敏感性为1×105拷贝/μL,第2次扩增的敏感性为1×102拷贝/μL,第2次扩增的敏感性比第1次高103倍;该方法对山东省各地采集的24份疑似病料可检出22份阳性。表明本试验所建立的套式PCR方法可用于鸭黄病毒感染的临床诊断和流行病学调查。 相似文献
12.
After a more general mention of Egg drop Syndrome 76 (EDS 76) an account is given of 3 cases of EDS 76 in broiler parent flocks. In all cases the animals involved originated from Ireland and were imported as day-olds, either directly from Ireland or via Sweden. Serological examinations indicate that the infectious agent has not previously been present in Danish hen flocks, but that it is presumably stationary in Danish flocks of ducks. In 2 of the 3 cases of EDS 76 animals from 2 different foreign firms were involved, and everything indicates that the infection has been carried by chickens from one of the firms and spread horizontally to the other. In the first case 12 weeks lapsed from the first to the last of 6 flocks in the same locality were attacked by SDS 76. An example is also given of trans-ovarial spread of EDS 76-virus to broiler flocks which at the age of 10--20 days were attacked by a disease similar to gangrenous dermatitis. In the course of the following few weeks an intensive rise took in the frequency of positive reaction to EDS 76. It is assumed that the EDS 76-virus has been of importance to the development of the said disease in these broiler flocks. 相似文献
13.
根据GenBank公布的鸡产蛋下降综合征病毒六邻体蛋白基因的高度保守序列,设计了2对特异性引物和1条TaqMan探针.以构建的阳性重组质粒为标准品,绘制标准曲线,建立了一种快速检测鸡产蛋下降综合征病毒的TaqMan荧光实时定量PCR方法.该方法最小检出量达10 copies·μL-1,在1.0×102～1.0×108copies· μL-1检测范围间有良好的线性关系,特异性、稳定性和重复性也较好.用建立的本方法检测感染鸡产蛋下降综合征病毒鸡群的产蛋分离物,与普通PCR相比,该荧光实时定量PCR方法具有更高的敏感性,可更好地用于鸡产蛋下降综合征病毒的临床检测. 相似文献
14.
Mycoplasma gallisepticum vaccination: effects on egg transmission and egg production 总被引：1，自引：0，他引：1
The effects of Mycoplasma gallisepticum (MG) vaccination on egg transmission of MG and egg production were evaluated. Leghorn hens vaccinated with live MG (strain F), with strain F plus MG bacterin, with one dose of MG bacterin, or with two doses of MG bacterin all transmitted MG through the egg at a significantly lower level than unvaccinated controls. Hens vaccinated with two doses of MG bacterin had the longest lag before detectable transmission of MG through the egg. All vaccinated groups were protected against the egg-production drop seen in unvaccinated hens challenged with virulent MG. 相似文献
15.
Mycoplasma gallisepticum vaccination: further studies on egg transmission and egg production 总被引：2，自引：0，他引：2
Leghorn hens vaccinated twice with an inactivated Mycoplasma gallisepticum (MG) bacterin before egg production and subsequently challenged with virulent MG were protected against transmission of MG through the egg. Unvaccinated control hens transmitted MG through the egg at a high rate. When unvaccinated hens were vaccinated with MG bacterin 2 weeks after challenge with MG, there was no significant decrease in egg transmission. Hens vaccinated twice before laying did not suffer as severe egg-production drops as unvaccinated hens did when challenged with virulent MG. In a natural MG outbreak in a leghorn breeder flock, 50 hens were separated from the remainder of the flock and vaccinated with MG bacterin approximately 3 weeks after initial exposure. The unvaccinated hens transmitted MG through the egg at a rate three times higher than the rate of transmission of the vaccinated hens. 相似文献
16.
An investigation of poor laying performance in a flock of free-range hens revealed high levels of serum antibodies to EDS 76 in the flock initially examined and in another, older flock on the same farm. These flocks had contact with ducks on a farm dam and were supplied with untreated drinking water from the dam. Serological evidence indicated that another flock supplying the same egg packing station had been infected with EDS 76 virus. Little serological evidence of EDS infection was detected from five other flocks supplying the packing station, parent breeders or the ducks resident on the dam. Therefore, the source of the EDS 76 virus remains conjectural. 相似文献
17.
An egg drop associated with splenomegaly in broiler breeders 总被引：2，自引：0，他引：2
A new disease in broiler breeders known in Australia as Big Liver and Spleen Disease (BLS) is described from field observations, retrospective record analysis, and detailed study of a selected flock. BLS has a predilection for adult birds. It is characterized clinically by a sudden drop in egg production, splenomegaly, hepatomegaly, and increased mortality and histologically by a period of lymphoproliferation followed by a period of lymphoid destruction that coincides with the clinical signs. Epidemiology suggests an infectious cause, though initial attempts at isolation of a causative agent have been unsuccessful, and it is serologically distinct from the common avian viral and mycoplasma diseases. 相似文献
18.
Sugiyama M Koimaru H Shiba M Ono E Nagata T Ito T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):783-787
Decreases in egg production and increased incidence of abnormal eggs due to malformation of egg shells were observed in specific pathogen free (SPF) 173-day-old laying hens inoculated intravenously with an avian metapneumovirus (aMPV) strain PLE8T1. This strain was derived from an isolate from broiler birds exhibiting swollen head syndrome (SHS). Some SPF birds inoculated with the virus showed, slight diarrhea without any respiratory symptoms. Thus, the PLE8T1 strain was used as a challenge virus to evaluate efficacy of aMPV vaccines. SPF chickens which received a live attenuated aMPV vaccine (NEMOVAC; Merial) at 7 or 77 days old and an inactivated aMPV vaccine (OVO-4; Merial) at 105 days old were protected against poor egg production caused by the challenge with the PLE8T1 strain. Thus, aMPV, the PLE8T1 strain passaged 22 times after isolation, from birds exhibiting SHS, could induce a drop in egg production in laying hens accompanied by malformation of egg shells. It was suggested that this challenge system could be applied to evaluate the efficacy of aMPV vaccine. 相似文献
19.
The immunogenicity of the ts-11 vaccine strain of Mycoplasma gallisepticum was assessed following eye drop or coarse aerosol administration in chickens of various ages. Protection was evalualted following intra-abdominal (IA) or fine droplet aerosol administration of virulent M. gallisepticum, usually the Ap3AS strain and was measured mainly by the scoring of gross air sac lesions or by egg production. Vaccination of chickens with ts-11 did not elicit a substantial serum antibody response as measured by rapid serum agglutination test, or ELISA. Protection was never demonstrated when no M. gallisepticum serum antibody response was detected in a vaccinated group of chickens. Failure to protect occurred usually, although not invariably, following aerosol administration of the vaccine. Vaccination by eye drop usually, although not invariably provided protection against challenge. In one experiment, chickens vaccinated by eye drop at 8-weeks were as susceptible as non vaccinated controls when challenged by IA inoculation at 13-weeks-of-age. Yet other birds from the same vaccinated group were resistant when challenged in an identical way at 23-weeks. No measurable increase in M. gallisepticum specific serum antibody concentrations occurred in the intervening period. Equally surprising was the response of another group of birds in the same experiment that had been vaccinated with a higher dose of ts-11. An antibody response was detected in this group, but they were susceptible to challenge at 23-weeks. Interestingly, a drop in egg production commenced 4 weeks after challenge, 2 weeks later than that observed in a non vaccinated group challenged at the same time.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
20.
采用猪瘟病毒C株毒（CSFV C strain）免疫BALB/C小鼠,按照常规单克隆抗体制作方法构建并筛选出能稳定分泌抗猪瘟单克隆抗体杂交瘤细胞株4株,分别命名为1C9,1B11,3C8和3G9,经鉴定,4株单克隆抗体腹水效价达到104~106。交叉试验及特异性试验表明,所制备的McAb与鸽新城疫病毒、猪蓝耳病病毒、猪传染性胃肠炎病毒、鸡减蛋综合征病毒无交叉反应性,均完全针对猪瘟病毒（CSFV）抗原决定簇,具有高度特异性。抗CSFV McAb的成功制备,为进一步研究建立CSFV的快速诊断方法奠定了基础。 相似文献