共查询到19条相似文献，搜索用时 234 毫秒
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灵芝提取物对人肝癌SMMC—7721细胞DNA合成的影响 总被引：6，自引：0，他引：6
本文以人肝癌ＳＭＭＣ－７７２１细胞为材料，对水溶性灵芝提取物（ＥＧＬ）抗癌作用机理进行了研究。结果表明，ＥＧＬ能明显抑制ＳＭＭＣ－７７２１细胞的ＤＮＡ合成；ＥＧＬ在５０ｕｇ／ｍｌ到３０ｕｇ／ｍｌ范围表现出明显的剂量效应；ＥＧＬ最小有效作用剂量为１００ｕｇ／ｍｌ，最大有效作用剂量为３００ｕｇ／ｍｌ。结果提示ＥＧＬ的抗癌机理主要是通过抑制癌细胞ＤＮＡ合成而起作用的。 相似文献
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双孢蘑菇子实体多糖的提取及其对癌细胞的抑制 总被引：13，自引：1，他引：12
双孢蘑菇子实体（Ａｇａｒｉｃｕｓｂｉｓｐｏｒｕｓ）经热水提取，乙醇沉淀，胰蛋白酶消化，Ｓｅｖａｇ法去除蛋白质等处理，得多糖。经紫外可见光分光光度计测定无紫外吸收峰，为较纯多糖。并研究了其对人肝癌ＳＭＭＣ－７７２１细胞生长曲线、有丝分裂指数及线粒体活性的影响，结果表明该多糖对体外培养的人肝癌ＳＭＭＣ－７７２１细胞增殖有一定抑制作用，抑癌实验证明该多糖对小鼠Ｓ－１８０实体瘤亦有一定抑制作用。 相似文献
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自由基清除剂对延缓青花菜花蕾衰老的效应 总被引：20，自引：2，他引：18
青花菜采后花蕾迅速衰老，叶绿素和蛋白质含量明显下降，超氧物歧化酶（ＳＯＤ）和过氧化氢酶（ＣＡＴ）活性随衰老进程先升高而后下降，丙二醛（ＭＤＡ）持续增加，细胞膜透性迅速增大。２０ｍｇ／Ｌ６－苄基腺嘌呤（６－ＢＡ）处理明显延缓叶绿素和蛋白质的降解，提高ＳＯＤ活性，对ＣＡＴ激活尤为明显，抑制了Ｈａｂｅｒ－Ｗｅｉｓｓ反应，降低过氧化氢（Ｈ２Ｏ２）和羟基自由基（ＯＨ）含量，延缓膜脂过氧化作用，降低子ＭＤＡ含量，减小膜渗漏，延缓花蕾衰老。２０ｍｇ／Ｌ６－ＢＡ中加入０２％苯甲酸钠（ＳＢＮ），对６－ＢＡ产生的各种延衰效应均有增效作用，进一步推迟花蕾衰老。２００ｍｇ／Ｌ抗坏血酸（ＡｓＡ）促进叶绿素和蛋白质降解，在提高ＳＯＤ和ＣＡＴ活性的同时，ＭＤＡ反而明显增加，膜透性迅速增大，加速衰老。 相似文献
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哈椒４号是用ＳＬＡ－０４作母本，ＳＬＧ－１６作父本配制的早熟一代杂种，从定植至始收需２０～２５天。果深绿色，光滑，３～４心室，肉厚０．２５ｃｍ，长１０．２ｃｍ，横径８．５ｃｍ。株型紧凑，生长势强，适宜密植，连续坐果力强，抗ＴＭＶ，耐ＣＭＶ和疫病，平均产量２３００ｋｇ／６６７ｍ２。 相似文献
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苹果褪绿叶斑病毒提纯及抗血清制备技术研究 总被引：12，自引：0，他引：12
本试验比较了２种提取缓冲液和３种繁殖寄主对苹果褪绿叶斑病毒（ＡＣＬＳＶ）提纯效果的影响。以昆诺藜（Ｃｈｅｎｏｐｏｄｉｕｍｑｕｉｎｏａ）和苋色藜（Ｃ．ａｍａｒａｎｔｉｃｏｌａｒ）作繁殖寄主,经蔗糖密度梯度和硫酸铯平衡密度梯度离心,获得了高度纯化的病毒样品。其Ａ２６０／Ａ２８０＝１．５７,产量约为５μｇ／ｇ叶,病毒粒体呈柔软线状。用提纯病毒免疫大白兔制备出抗该病毒的抗血清。免疫电镜观察,该抗血清对ＡＣＬＳＶ粒体具明显的修饰作用。采用双抗体夹心法（ＤＡＳ－ＥＬＩＳＡ）和Ａ蛋白酶联法（ＰＡＳ－ＥＬＩＳＡ）分别对１８个桃树和１７个梨树样品进行检测,阳性与阴性样品吸光值差异明显。 相似文献
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AIM: To explore the different inhibitory effect of arsenic trioxide (As2O3) on hepatocarcinoma cell growth in SMMC-7721 and BEL-7402 cell lines and its mechanism. METHODS: The cell culture and trypan blue staining were used to study the inhibitory effect of arsenic trioxide on cell growth, and the glutathione (GSH) contents in hepatocarcinoma cells treated with arsenic trioxide were detected. RESULTS: Arsenic trioxide inhibited the growth of BEL-7402 cells in a time and dose-dependent manner. The inhibitory effect was significant at a lower dose of 0.50 μmol/L for 24 h, however, to SMMC-7721 cells, a higher dose of 2.00 μmol/L for 96 h was needed. The inhibitory rate of arsenic trioxide (0.25-2.00 μmol/L) on BEL-7402 cell growth was higher than that on SMMC-7721 cells. The content of GSH in SMMC-7721 cells was much higher than that in BEL-7402 cells . CONCLUSION: There was a significant difference in inhibition of hepatocarcinoma cell growth by arsenic trioxide between BEL-7402 and SMMC-7721 cell lines, the cause of which may be due to the difference in GSH content in BEL-7402 and SMMC-7721 cells. 相似文献
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AIM: To investigate the effects of 2-deoxy-D-glucose (2-DG) or oxaliplatin (L-OHP) alone and the combination of both on the proliferation and apoptosis of hepatoma cell line SMMC-7721 in vitro. METHODS: Human hepatoma cell line SMMC-7721 was treated with 2-DG or L-OHP alone, or both. The inhibitory effect on the proliferation of SMMC-7721 cells was estimated by MTT method. The q value, which represents synergistic effect, was determined. Apoptotic rate and cell cycle were assayed by flow cytometry. The activity of caspase-3 was detected by a caspase-3 activity assay kit. RESULTS: 2-DG or L-OHP at different concentrations inhibited the growth of SMMC-7721 cells obviously and the inhibitory effect on SMMC-7721 cell growth strongly depended on the exposure time and dose. When the 2 drugs worked together, the inhibitory effect was improved (P<0.05). 2-DG induced cell apoptosis and arrested the cells at G2/M phase. When combined with L-OHP,the 2 drugs induced more severe apoptosis and arrested the cells at S and G2/M phase. Meanwhile, the activity of caspase-3 increased when the 2 drugs used together. CONCLUSION: 2-DG inhibits the growth of hepatoma cell line SMMC-7721. The combination of 2-DG and L-OHP improves the ability of L-OHP to attack the tumor cells. The mechanism might be related to increasing the activity of caspase-3. 相似文献
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PENG Lin-hui ZHOU Jie HUO Feng PU Miao-shui ZHANG Qi SU Chang-qing QIAN Qi-jun WAN Yun-le 《园艺学报》2012,28(5):802-806
AIM: To investigate the cytotoxicity and mouse IFN-γ (mIFN-γ) expression of oncolytic adenovirus CNHK300-mIFN-γ (CNHK300-Mγ) containing mIFN-γ gene in malignant tumor cells in vitro . METHODS: Human lung cancer cell line A549, human liver cancer cell line SMMC-7721, human pancreatic cancer cell line PANC-1, and human normal fibroblast line BJ were cultured and treated with CNHK300-Mγ, CNHK300, ONYX-015 or AdEasy-mIFN-γ (AdEasy-Mγ). TCID50 assay was used to evaluate the replication ability of CNHK300-Mγ, CNHK300 and ONYX-015 in carcinoma cell lines and normal cell line, and the cytotoxicity was evaluated by cytopathic effect assay and MTT assay. The mIFN-γ expression in the supernatant was detected by ELISA after CNHK300-Mγ or AdEasy-Mγ infection in carcinoma cell lines and normal cell line. RESULTS: The tumor-specific replication ability and cytotoxicity of CNHK300-Mγ were similar to those of CNHK300. The IC50 was as low as MOI of 0.47 pfu/cell for A549 cells, 0.074 pfu/cell for SMMC-7721 cells, 0.532 pfu/cell for PANC-1 cells and was as high as MOI of 281.73 pfu/cell for BJ cells. CNHK300-Mγ was a more powerful killer of malignant tumor cells than ONYX-015 (P<0.01). The tumor cells infected with CNHK300-Mγ efficiently expressed mIFN-γ in vitro and mIFN-γ largely increased as the time prolonged in A549, SMMC-7721 and PANC-1 cells. The mIFN-γ expression in the carcinoma cell lines infected with CNHK300-Mγ was much higher than that in the cells infected with AdEasy-Mγ (P<0.01), but was similar to that in the normal cell line (P>0.05). CONCLUSION: CNHK300-Mγ selectively replicates and effectively promotes the expression of mIFN-γ in carcinoma cells, and specifically kills the tumor cells. 相似文献
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HUANG Tao ZHOU Qi GONG Dong-wei GAO Quan-li ZHANG Xu-hua LV Xiao-dong ZHOU Jin-xue 《园艺学报》2011,27(3):523-527
AIM: To study the biological characteristics of side population (SP) cells sorted from hepatoma SMMC-7721 cell line. METHODS: Fluorescence-activated cell sorter (FACS) was used to sort SP cells and non-SP (NSP) cells from SMMC-7721 cell line. The colony-formation ability and proliferation ability between SP cells and NSP cells were compared in terms of plate colony assay and growth curve. The migratory and invasive properties of SP cells and NSP cells were tested by Transwell method. The cell cycle and apoptosis were analyzed by flow cytometry. The oncogenicity of the cells was analyzed by nude mouse transplantation tumor experiment in vivo. RESULTS: The results of FACS analysis indicated that (9.2±0.2)% of the SMMC-7721 cells were SP cells. The proportion of G0/G1 phase of SP cells was higher, and the apoptotic rate was lower than those of NSP cells (P<0.05). The proliferation ability and colony-forming ability and migratory and invasive properties of SP cells were significantly higher than those of NSP cells (P<0.05). The nude mouse transplantation tumor experiment displayed that the oncogenicity of SP cells was higher than that of NSP cells. CONCLUSION: The SP cells sorted from SMMC-7721 cell line may enrich tumor stem cells. 相似文献
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BAI Hui-li LI Bao-lin HE Fang ZHANG Ru-yi YAN Shu-juan LIU Chen YANG Dan-dan SHI Qiong 《园艺学报》2013,29(11):1921-1927
AIM: To investigate the effects of marrow stromal HS-5 cells on hepatocellular carcinoma SMMC-7721 cells in the tumor microenvironment. METHODS: The effects of HS-5 cell-conditioned medium (HS-5-CM) on the proliferation, migration and invasion abilities of SMMC-7721 cells were detected by MTT, wound-healing and Transwell assays. After co-culture of SMMC-7721 cells with HS-5 cells in the Transwell chamber, the expression of chemokine CCL5 and its receptor CCR5 at mRNA and protein levels in SMMC-7721 cells was examined by quantitative real-time PCR (qRT-PCR), ELISA or Western blotting. Akt and p-Akt473 protein levels in SMMC-7721 cells treated with PI3K inhibitor LY294002 were observed by Western blotting. RESULTS: HS-5-CM promoted the proliferation, migration and invasion abilities of SMMC-7721 cells. The expression of CCL5 and CCR5 at mRNA and protein levels in SMMC-7721 cells was increased after co-cultured with HS-5 cells. PI3K inhibitor LY294002 inhibited the activation of PI3K-Akt signaling pathway and the secretion of CCL5 in SMMC-7721 cells after co-cultured with HS-5 cells. CONCLUSION: HS-5 cells significantly promote the proliferation, migration and invasion abilities of SMMC-7721 cells. Co-culture of SMMC-7721 cells with HS-5 cells activates PI3K-Akt signaling pathway to increase the secretion of CCL5 in SMMC-7721 cells. 相似文献
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AIM:To investigate the antitumor activity and mechanism of Paecilomyces tenuipes polysaccharide(PTPS).METHODS:PTPS-I was obtained by water extraction and alcohol precipitation, and purified by DEAE-cellulose and Sephadex G-100 chromatography. Human erythroleukemia cell line K562, laryngocarcinoma cell line Hep2 and hepatic carcinoma cell line SMMC-7721were co-cultured with PTPS-I or the conditioned medium which prepared with PTPS-I-stimulated human mononuclear cells (PTPS-I-MNC-CM), and the proliferation of tumor cells was determined. The cell counting kit-8 (CCK-8) was used to determine the proliferation of MNCs. The FQ-RT-PCR was applied to investigate the expression of TNF-α and IL-6 mRNA in MNCs. RESULTS:PTPS-I-MNC-CM inhibited the proliferation of K562, Hep2 and SMMC-7721 cells in vitro (P<0.01). Cytotoxicity of PTPS-I against K562, Hep2 and SMMC-7721 cells was not observed (P<0.01). PTPS-I stimulated the proliferation of MNCs (P<0.01) and significantly enhanced the expression of TNF-α and IL-6 mRNA in MNCs (P<0.05, P<0.01). CONCLUSION:The results suggest that PTPS-I is an immunomodulator and its antitumoral activity is through the immunomodulatory mechanism rather than the direct cytotoxicity against tumor cells. 相似文献
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AIM: To investigate the expression of microRNA-141 (miR-141) in human hepatocellular carcinoma (HCC) cell line SMMC-7721 and normal hepatocyte line HL-7702, and to analyze the effect of abnormal expression of miR-141 on the malignant biological behaviors of human hepatocarcinoma cells. METHODS: The RNA from SMMC-7721 cells and HL-7702 cells was extracted. SYBR Green real-time PCR was performed to detect the expression of miR-141. Synthetic miR-141 mimic and its negative control were transfected into the SMMC-7721 cells, and miR-141 inhibitor and its negative control were transfected into the HL-7702 cells by the method of Lipofectamine. After transfection, MTS assay and BrdU-ELISA were employed to evaluate the effect of miR-141 on the cell proliferation. Flow cytometry was used to detect cell cycle and apoptosis. The changes of migration ability were investigated by Transwell invasion assay. RESULTS: The expression of miR-141 in the SMMC-7721 cells was significantly lower than that in the HL-7702 cells (P < 0.05). Compared with blank group, Lipofectamine group and negative control group, the proliferation of the SMMC-7721 cells transfected with 25 nmol/L miR-141 mimic was significantly inhibited in a time-dependent manner (P < 0.05). The percentages of G1 phase cells and early apoptotic rate were significantly increased when miR-141 was up-regulated, but the migration ability was inhibited (P < 0.05). Compared with blank group, Lipofectamine group and negative control group, the proliferation of HL-7702 cells transfected with 50 nmol/L miR-141 inhibitor was significantly increased in a time-dependent manner (P < 0.05). When miR-141 was down-regulated, the percentages of G1 phase cells and early apoptotic rate were significantly decreased, but the migration ability was enhanced (P < 0.05). CONCLUSION: miR-141 is down-regulated in human hepatocarcinoma cell line. Up-regulation of miR-141 will not only inhibit cell proliferation and migration ability, but also affect the cell cycle and apoptosis of SMMC-7721 cells. miR-141 may function as a tumor suppressor gene during HCC development. 相似文献
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AIM: To observe the influence of high expression of miR-15a-5p on the proliferation and migration of human hepatocellular carcinoma SMMC-7721 cells.METHODS: The miR-15a-5p oligonucleotide, which was reconstructed with additional restriction sites of EcoR Ⅰ and Hind Ⅲ, was chemically synthesized and confirmed by sequencing. The miR-15a-5p eukaryotic expression system was constructed by pcDNA6.2-GW/Em-GFP-pre-miR-15a-5p plasmid. The miR-15a-5p was transfected into the SMMC-7721 cells transiently by plasmid, and quantified by quantitative real-time PCR at the mRNA level. The cell viability was measured by CCK-8 assay, and the living cell counting was performed by the method of Trypan blue exclusion. The migration ability of the SMMC-7721 cells with high expression of miR-15a-5p was detected by wound healing test.RESULTS: The sequence of miR-15a-5p oligonucleotide 100% matched the designed sequence. Compared with control group, the miR-15a-5p expression was increased significantly (P<0.05). The viability, the living cell number and the migration ability of the SMMC-7721 cells were decreased in high expression of miR-15a-5p group with statistically significant difference (P<0.05).CONCLUSION: The abilities of proliferation and migration in human hepatocellular carcinoma SMMC-7721 cells are decreased by high expression of miR-15a-5p. 相似文献