共查询到20条相似文献，搜索用时 984 毫秒
1.
陆地棉耐盐性状与SSR分子标记的关联分析 总被引：1，自引：1，他引：1
本研究以134份陆地棉栽培种为试验材料,测定其在0.3%盐浓度(质量分数)下的出苗率,并使用74对SSR引物对这些材料进行基因组变异扫描。利用Structure2.3.4软件分析该自然群体的遗传结构,在此基础上采用Tassel2.1软件对耐盐性状与SSR分子标记进行关联分析,寻找与棉花耐盐性状相关的分子标记。研究结果表明:(1)134份陆地棉栽培种的出苗率呈极显著差异,并筛选出27个盐敏感材料和10个耐盐材料。(2)74个SSR分子标记共检测出148个多态性位点,涉及246个等位变异,变异范围为2~7,平均每个标记3.32个;基因多样性指数变异范围为0.0295~0.4959,平均值为0.2897;SSR分子标记多态性信息含量(PIC)变幅为0.0290~0.3729,平均值为0.2381。(3)通过群体结构分析,将该自然群体划分2个亚群体,分别包含89份和45份材料。(4)关联分析共发现8个与棉花耐盐性状相关的SSR分子标记位点,表型变异解释率变幅为2.91%~7.82%,平均值为4.32%。此研究结果可以为棉花耐盐性状分子标记辅助选择育种提供参考。 相似文献
2.
陆地棉繁殖群体大小对遗传完整性的影响 总被引：1，自引：0，他引：1
利用形态标记和SSR分子标记技术分析了澳L23/757(鸡脚叶)、中棉所19(正常叶)、红桃(红叶)的25、50、100、150株不同自然授粉繁殖群体的遗传完整性。结果表明,3个种质间的形态标记的天然异交率和SSR标记遗传纯度有显著差异,但4个群体大小间的天然异交率和SSR标记遗传纯度的差异均不显著。说明棉花繁殖群体为25株时,可作为棉花种质繁殖更新过程中的最低繁殖群体量,能较好地保持繁殖种质的遗传完整性。 相似文献
3.
4.
小麦分子遗传图谱的加密 总被引：2，自引：1，他引：1
高密度的分子标记遗传图谱是QTL定位、图位克隆和分子标记辅助选择等研究的基础。以小麦品种“京花1号/小白冬麦”的双单倍体(DH)群体和“农大015/复壮30”的重组自交系(RIL)群体为作图群体,选用在DH群体双亲间的339个多态性标记和在RIL群体双亲间的343个多态性标记分析作图群体各个株系的基因型,对本中心近年开发的SCAR、EST-SSR标记以及他人开发的SSR、EST-SSR标记进行了染色体定位,并利用连锁分析软件Joinmap 4.0将2个作图群体的结果整合,最终构建了10个连锁群,将217个SSR、EST-SSR和SCAR位点定位在9条染色体上,进一步提高了小麦遗传图谱的密度。 相似文献
5.
甜瓜果肉颜色是影响消费者选择的重要果实品质性状之一。为了研究甜瓜果肉颜色性状的遗传规律,以‘库克拜热’(绿肉)与‘西州蜜’(橙肉)构建BC1群体,‘库克拜热’与‘皇后’(橙肉)构建F2群体,利用集群分离分析(bulked segregation analysis, BSA)与简单重复序列(simple sequence repeats, SSR)分子标记相结合的方法,明确了在上述甜瓜品种中,果肉颜色受两个基因的调控,其中橙色果肉控制基因gf对白色果肉控制基因wf具有显性上位作用,绿色对白色为隐性。本研究同时开发出相应的多态性分子标记,其中分子标记SSR2021、SSR2103与gf紧密连锁,分子标记SSR2956、SSR2980与wf紧密连锁,可用于区分橙色、白色和绿色果肉甜瓜。本研究为不同果肉颜色甜瓜的选育提供一定的理论基础和技术支持。 相似文献
6.
陆地棉7个置换系的遗传评价 总被引：11，自引：0，他引：11
对7个陆地棉置换系的形态、产量、纤维品质及个体发育等性状进行了系统研究,结果表明所研究的7个置换系分别与不同的农艺性状相关联,同一农艺性状同时与几个置换系相关联.利用(TM-1×Sub16)F2群体进行分子标记筛选,标记SSR1694195与第一果枝节位、铃重、衣指连锁,标记SSR2986120和SSR300880均与铃重、开花日期连锁,标记SSR306 相似文献
7.
《分子植物育种》2017,(7)
为了研究花生SSR分子标记与品质性状之间的关系,本研究以农家品种四粒红和冀农黑3号杂交产生的251个重组自交系(RIL)群体为材料,运用SPSS17.0软件分析花生RIL群体品质性状与SSR分子标记的相关性。结果表明多数品质性状间有极显著或显著相关的关系。棕榈酸和油酸的相关系数最高为(r=-0.887),其次为硬脂酸和油酸(r=-0.753)。Pearson’s相关分析表明77个分子标记与8个品质性状存在着显著相关性。运用多元回归分析得到45个与8个品质性状相关的标记,解释的表型总变异的范围为8.1%~28.5%。上述结果为利用分子标记辅助选择改良花生的品质性状提供了参考。 相似文献
8.
9.
柱花草重要性状与SSR、SRAP分子标记的关联分析 总被引：1，自引：0，他引：1
《分子植物育种》2017,(5)
为了寻找与柱花草重要性状相关联的分子标记,本研究以20份柱花草品种(系)为对象,进行了株高等5个农艺性状和粗蛋白等8个品质性状的观测分析。在分析遗传多样性和群体结构的基础上,将品种间差异显著的11个性状与SSR、SRAP标记进行关联分析。结果表明,共有14个SSR标记与5个性状相关联,有16个SRAP标记与4个性状相关联。其中,1个SSR标记、15个SRAP标记与鲜重相关联;12个SSR标记和1个SRAP标记与株高相关联;12个SSR标记、1个SRAP标记与茎粗相关联;11个SRAP标记与粗蛋白含量相关联,分别只有1个SSR标记与叶宽、粗灰分含量相关联。关联分析能够有效地找到与部分性状相关联的标记,为柱花草种质鉴定、杂交育种及分子育种实践提供依据。 相似文献
10.
基于AFLP和SSR标记的高粱分子遗传连锁图构建 总被引：3，自引：0，他引：3
以茎秆糖份含量高的高粱自交系1095和低糖高粱自交系N3杂交获得的F2分离群体(205个个体)为材料,采用AFLP(amplified fragment length polymorphism)和SSR(simple sequence repeat)两种分子标记,构建了包含273个(232AFLP,41 SSR)标记,覆盖基因组长度为978.1cM的高粱分子标记连锁遗传图.以SSR标记为锚标记,19个连锁群中,18个连锁群各自被归并于高粱的10个连锁群(A-J)中.该连锁图平均图距和最大图距分别为3.6 cM和19.4 cM,未出现大的空隙(gap＞25 cM),归并后的10个连锁群(A-J)分别对应于高粱染色体SBI-01、SBI-02、SBI-03、SBI-04、SBI-07、SBI-09、SBI-10、SBI-08、SBI-06、SBI-05. 相似文献
11.
以58份不同类型(野生、半野生和栽培)大豆种质为材料,利用32对SSR标记分析大豆种质间的遗传多样性和进化关系,采用NIRS和GC方法分别分析大豆脂肪含量和脂肪酸组分含量,研究不同类型大豆种质油脂组成特点及其与演化的关系。结果显示,野生大豆和栽培大豆的油脂组成存在显著差异,栽培大豆脂肪含量(平均20.8%)显著高于野生大豆(平均10.49%),油酸含量(平均28.5%)显著高于野生大豆(平均14.37%),而亚麻酸含量却显著低于野生大豆;由相关性分析可知,大豆种子中的脂肪与油酸含量显著正相关(r=0.85**),而与其他脂肪酸组分极显著负相关;油酸与所有其他脂肪酸组分均负相关,特别是与亚麻酸和亚油酸呈极显著负相关(r=?0.90**和?0.89**);油脂组成和SSR标记对不同类型大豆种质的聚类和主成分分析表明,2种分类结果基本一致,可分为栽培和野生2个亚群,半野生大豆则分布于2个亚群中。由此可见,大豆油脂组成与大豆种质的驯化程度有关,脂肪含量和亚麻酸含量可以作为大豆演化分类的参考指标。 相似文献
12.
Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape (Brassica napus) 总被引：1，自引：0，他引：1
The quality of the oil derived from oilseed rape is determined by its fatty acid composition. Breeding oilseed rape for enhanced oil quality includes the development of cultivars with high oleic and low linolenic acid. Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) techniques were investigated for the development of molecular markers for genes controlling oleic and/or linolenic acid. Markers that were identified were converted to sequence characterized amplified region (SCAR) markers for use in breeding. Molecular markers associated with these two fatty acids were identified in a doubled haploid population derived from a cross between the oilseed rape lines TO99‐5318‐20, very high oleic (>79%) and very low linolenic acid (<2%) × DH12075, high oleic (68%) and higher linolenic acid (>7%). Eight RAPD markers were associated with oleic and linolenic acid contents. The RAPD marker UBC 2830 accounted for 43% and 13% of the genetic variation for oleic and linolenic acid levels, respectively. The RAPD marker UBC 153550 accounted for 19% of the genetic variation for linolenic acid. The UBC 2830 fragment was converted to a SCAR marker. The markers identified in this study should be useful tools for the early generation selection of high oleic and low linolenic acid genotypes in oilseed rape breeding programmes. 相似文献
13.
大豆脂肪酸主要组分含量QTL定位 总被引：2，自引：1，他引：1
以中黄13×中黄20的100个BC2F2家系为作图群体,构建了一张包含131个SSR分子标记的遗传连锁图谱,图谱总长为2157.3 cM,平均遗传距离为16.5 cM,涵盖了大豆的20个连锁群。利用气相色谱技术测定BC2F2、BC2F3和BC2F4回交群体的脂肪酸主要组分含量,采用IciMapping 3.3完备区间作图法定位QTL,共检测到5种脂肪酸组分相关的QTL 26个,与棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸相关的QTL分别为5、5、7、5和4个;3个区间在不同年份被检测到与同一脂肪酸组分相关,sat_294~satt228连续3年被检测到与棕榈酸含量相关,sat_253~satt323和sat_292~satt397连续2年被检测到与油酸含量相关;4个区间被检测到与2种脂肪酸组分相关,其中sat_294~satt228与棕榈酸、油酸相关,satt308~sat_422与硬脂酸、亚油酸相关,sat_292~satt397与油酸、亚油酸相关,satt374~satt269与油酸、亚麻酸相关。 相似文献
14.
SCAR and RAPD markers associated with 18-carbon fatty acids in rapeseed, Brassica napus 总被引：4，自引：0，他引：4
Breeding rapeseed for enhanced oil quality includes the development of varieties with low linolenic acid content. The breeder also aims to develop varieties with a high linoleic acid content because of its nutritional value. Restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers have been developed for linolenic acid content, but they are not best suited for a direct application in marker-assisted selection. The RFLP technique is too complex and time-consuming and RAPD markers lack codominance, precluding the distinction of homozygous from heterozygous individuals. In this report the conversion of a RAPD marker to a codominant sequence characterized amplified region (SCAR) marker named L1L9 is described. One of the alleles consisting of an 899 bp fragment (allele A), is associated with low linolenic acid content. The other allele consists of an 641 bp fragment (allele B) and is associated with high linolenic acid content. This marker explains approximately 25% of the genetic variation for this trait. Linkage analysis in the mapping population indicates that the SCAR marker probably tags an ω-3 desaturase gene in B. napus. Two RAPD markers were found to be associated with oleic/linoleic acid content. Markers M14-350 and I06-650 explained approximately 10% and 7% of the genetic variation for linoleic acid content, respectively. These two markers were found linked at 12.3cM in the segregating B. napus F2 progeny used for mapping. All the markers reported in this paper should be useful in breeding programmes for developing high linoleic and low linolenic acid rapeseed varieties. 相似文献
15.
Undesirable characteristic of rapeseed oil is a relatively high level of linolenic acid (18:3), which is easily oxidized leading
to rancidity and a shortened shelf life of the oil. Previous attempts to reduce linolenic acid levels in rapeseed oil through
breeding have been impaired by complex genetics and strong environmental sensitivity of this trait. Therefore, our objective
was to develop molecular markers for low linolenic acid that could facilitate the breeding of low linolenic rapeseed. Bulked
segregant analysis was employed to identify two RAPD markers associated with 18:3 in a doubled haploid population segregating
for linolenic and erucic acid levels. Based on analysis of individual DH lines, the markers RM350 and RM574, representing
two independent loci, accounted for a total of 39% of the genetic variability in this population. This marker RM350 alone
accounted for 25% genetic variation for this trait with no evidence of recombination. Significant interlocus interaction found
between the markers RM350 and RM574 suggested that epistasis was involved in the genetic control of 18:3 level in this population.
Another marker designated as RM322, which was independent of the other two, was found significantly associated with the erucic
acid level and oil content. RAPD markers identified in this study should be a useful tool for the early detection of low linolenic,
or low or high erucic acid genotypes in rapeseed breeding programs based on doubled haploids.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
16.
大豆脂肪酸组分的胚、细胞质和母体遗传效应分析 总被引：1，自引：0，他引：1
利用5个大豆品种配制20个杂交组合,采用广义种子遗传模型分析了大豆脂肪酸组分的胚、细胞质和母体植株等3套遗传体系的基因主效应和基因型×环境效应。棕榈酸含量、硬脂酸含量和亚油酸含量是以基因型×环境互作效应为主。亚麻酸和油酸的遗传主效应和基因型×环境互作效应相近。在脂肪酸组分的遗传主效应中,棕榈酸、硬脂酸和亚油酸含量是以胚主效应为主。油酸含量和亚麻酸含量以细胞质主效应为主。在基因型×环境互作方差中,脂肪酸组分以极显著的胚互作方差为主。亚麻酸含量是以基因的加性效应和加性×环境互作效应为主,棕榈酸含量、硬脂酸含量、油酸含量和亚油酸含量以基因的显性和显性×环境互作效应为主。棕榈酸含量和油酸含量是以普通狭义遗传率为主。硬脂酸、亚油酸含量和亚麻酸含量以互作狭义遗传率为主。在普通狭义遗传率中,棕榈酸含量、油酸含量和亚麻酸含量以细胞质普通遗传率和母体普通遗传率为主。在互作狭义遗传率中,油酸含量和亚麻酸含量以胚互作狭义遗传率为主,亚油酸含量以母体植株互作遗传率为主。棕榈酸含量、硬脂酸含量、油酸含量和亚油酸含量以细胞质及母体选择响应和互作选择响应为主,亚麻酸含量的胚普通选择响应和互作选择响应为主。 相似文献
17.
Soybean [Glycine max (L.) Merr.] is the principal oilseed crop in the world. Soybean oil has various industrial and food applications. The quality of soybean oil is determined by its fatty acid composition. Palmitic, stearic, oleic, linoleic and linolenic are the predominant fatty acids in soybean oil. The objective of this study was to determine the associations of simple sequence repeat (SSR) molecular markers with minor differences in fatty acids in soybean oil thereby detecting modifier quantitative trait loci (QTL) which could further improve soybean oil quality. To achieve this objective, 101 F6-derived recombinant inbred lines (RIL) from a population whose parents did not contain major mutant fatty acid alleles were developed from a cross of N87-984-16 × TN93-99. Fatty acids were determined by gas chromatography. Heritability estimates on an entry mean basis for fatty acids ranged from 65.8 to 77.3% for palmitic and linoleic acids, respectively. Molecular marker Satt537 located on molecular linkage group (MLG) D1b was associated with palmitic acid and Satt168 and Satt249 located on MLG B2 and J, respectively were associated with stearic acid. Molecular markers Satt185 or Satt268 (which are within 0.6 cM of each other) located on MLG E were consistently associated with oleic and linoleic acid, and Satt263 and Satt235 located on MLG E and G, respectively were associated with linolenic acid. The lack of markers associated with multiple fatty acids suggests the possibility of independently changing fatty acid levels to achieve a desirable composition, except for regions common to all saturated fatty acids. Phenotypic variation explained by the fatty acids modifier QTL ranged from 10 to 22.5%. These modifier QTL may be useful in making minor improvements to further enhance the quality of soybean oil. 相似文献
18.
不同品种紫苏种子营养成分及脂肪酸组分分析 总被引：2，自引：1，他引：1
为了给优化种质资源改良提供理论依据,选用10个不同紫苏品种作为供试材料,研究其种子性状、品质、脂肪酸组成及含量,并对其相关性进行分析。结果表明,10个不同品种紫苏种子直径、千粒重、粗脂肪、粗蛋白和纤维素含量均存在显著差异,变幅为籽粒直径1.58~2.15 mm,千粒重2.36~5.12 g,粗脂肪34.0%~45.2%,粗蛋白18.5%~21.8%,纤维素19.0%~25.1%。总脂肪酸中含棕榈酸3.16%~4.77%,硬脂酸0.56%~1.86%,油酸11.42%~23.06%,亚油酸8.53%~13.02%,亚麻酸58.48%~70.90%,总不饱和脂肪酸占到总脂肪酸的93.26%~95.00%。ZY-3,ZY-1,ZB-1,BZ-2和BS-1粗脂肪含量高,α-亚麻酸比例大,可作为高油高亚麻酸优质育种材料。试验结果还表明,粗脂肪、亚麻酸含量与籽粒直径和千粒重间均呈显著正相关,油酸含量与籽粒直径和千粒重间呈显著负相关,而油酸与亚麻酸含量间呈极显著负相关。因此,要提高紫苏籽的亚麻酸含量,就要适当控制其油酸含量。 相似文献
19.
利用DH和IF_2群体检测油菜籽粒油酸、亚油酸、亚麻酸含量QTL 总被引：1，自引：0，他引：1
以德国冬性甘蓝型油菜Express和中国半冬性甘蓝型油菜SWU07为亲本构建包含261个株系的DH群体和包含234个株系的IF2群体,检测不同年份条件下油菜籽粒油酸、亚油酸、亚麻酸含量相关的QTL。在DH群体4年环境下共检测出71个QTL,在IF2群体2年环境下共检测出4个QTL。去掉在不同年份和群体中置信区间相互重叠的QTL之后,共得到3个品质性状的51个QTL,其中有15个在2年以上环境中被检测到。这些QTL分别分布在13个连锁群上,其中与油酸含量相关的18个,分布于A01、A02、A04、A05、A07、A09、C01连锁群,揭示3.44%～13.97%的表型变异;与亚油酸相关的12个,分布于A02、A06、A09、C01、C02连锁群,揭示3.84%～19.51%的表型变异;与亚麻酸相关的21个,分布于A01、A02、A03、A04、A05、A08、A09、C01、C02、C03、C06连锁群,揭示2.86%～11.91%的表型变异。这些结果将为油菜脂肪酸品质改良提供更多遗传信息。 相似文献
20.
Summary The fatty acid composition of seed-oil of breeding lines and F1 hybrids of Matthiola incana was analyzed, using direct esterification and gas chromatography. The breeding lines tested differed significantly with respect to the levels of palmitic, oleic, linoleic and linolenic acids. Embryonic-stage heterosis in linolenic acid concentration was demonstrated by F1 hybrid seeds, derived from mating horticulturally different lines of M. incana. Linolenic acid content was negatively correlated with both oleic acid content (r=–0.85) and linoleic acid content (r=–0.66). None of the breeding lines or the F1 hybrids significantly passed the limit of 67% linolenic acid. Possible genetic and biochemical explanations for the above phenotypic data are discussed. 相似文献