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土耳其斯坦叶螨对杀螨剂的抗性选育及解毒酶活力变化 总被引：3，自引：0，他引：3
为探索土耳其斯坦叶螨的抗药性及其生化机理,在室内对敏感系土耳其斯坦叶螨分别用螺螨酯、甲氰菊酯和阿维菌素逐代处理,选育出抗性种群。结果表明,选育至15代,土耳其斯坦叶螨对螺螨酯、甲氰菊酯和阿维菌素的抗性指数分别达到268.63、37.98和112.68倍。分别测定敏感品系(SS)、抗螺螨酯(RS)、抗甲氰菊酯(RF)、抗阿维菌素(RA)品系的解毒酶活性显示,3种不同抗性品系相对SS品系的羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFO)的比活力均有不同程度的提高,差异均达到显著水平(P0.05)。其中,RF品系的MFO比活力上升最快,是SS品系的12.7倍;RA品系的MFO比活力次之,是SS品系的5.76倍;RS品系的3种解毒酶比活力均增长较慢,其中CarE比活力上升最慢,是SS品系的1.31倍。由此表明,CarE、GSTs、MFO的活性增大可促进土耳其斯坦叶螨对3种杀虫剂的抗性形成;螺螨酯的抗性增强可能与CarE关系甚微;MFO活性的增加可能与甲氰菊酯抗性升高密切相关;GSTs、MFO的活性升高可能是土耳其斯坦叶螨对阿维菌素产生抗性的主要原因。 相似文献
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在室内模拟田间药剂的选择压力,用阿维菌素和甲氰菊酯对朱砂叶螨Tetranychus cinnabarinus 进行逐代处理,以选育其抗药性品系。阿维菌素品系选育至42代,抗性增长到8.7倍,甲氰菊酯品系选育至40代,抗性增长到68.5倍。阿维菌素抗性品系羧酸酯酶(CarE)、谷胱甘肽-S-转移酶(GSTs)、多功能氧化酶(MFO)的活性分别为敏感品系的2.7、3.4和1.4倍,差异达显著水平。推测3种解毒酶活性显著升高是朱砂叶螨对阿维菌素产生抗性的重要原因。甲氰菊酯抗性品系GSTs的活性为敏感品系的2.8倍,差异显著,表明该抗性品系的形成与GSTs活性增强有关。羧酸酯酶动力学测定结果表明,朱砂叶螨阿维菌素抗性品系体内存在变构的羧酸酯酶。 相似文献
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为了明确巴氏新小绥螨Neoseiulus barkeri溴氰菊酯抗性品系对常用杀虫(螨)剂的交互抗性水平及生物适合度,为巴氏新小绥螨抗药性品系田间应用提供科学依据。本文应用玻片浸渍法测定了巴氏新小绥螨溴氰菊酯抗性品系对12种杀虫(螨)剂的交互抗性,采用种群生命表评价了其生长发育及繁殖情况。结果显示,相对于巴氏新小绥螨敏感品系,溴氰菊酯抗性品系(226.38倍)对供试的其他6种拟除虫菊酯类药剂均存在交互抗性,尤其对氯氟氰菊酯的交互抗性倍数最高,达到2 210.91倍。对噻虫嗪、毒死蜱和乙螨唑的交互抗性分别是116.46、110.71倍和20.43倍,对丁硫克百威、阿维菌素和哒螨灵无交互抗性。对其生物适合度研究发现,溴氰菊酯抗性品系相较于敏感品系,前若螨期和产卵前期显著缩短,产卵期和单雌总产卵量显著增加。上述结果表明,巴氏新小绥螨溴氰菊酯抗性品系对10种常用杀虫(螨)药剂产生了不同水平的交互抗性,且其繁殖率显著增加,推荐在田间推广应用。 相似文献
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采自甘肃兰州兴隆山公园的二斑叶螨(Tetranychus urticae Koch),用雌雄单系培养敏感品系(S),用螺螨酯处理二斑叶螨种群培养抗性品系(SP R),用室内生测法对二斑叶螨S和SP R品系进行室内毒力测定。结果表明,二斑叶螨对螺螨酯抗性发展初期较慢,中期稳定,后期较快,选育至26代抗性指数(RI)达58.83。SP R对甲氰菊酯、氯氰菊酯有一定的交互抗性,RI分别为11.54和10.03;对苯丁锡、四螨嗪、苦皮藤生物碱、阿维菌素、氯氟氰菊酯、哒螨•四螨嗪、哒•水胺硫磷、三唑锡、三氯杀螨醇、哒螨灵、氧化乐果无交互抗性(1＜RI<5.00);对浏阳霉素、毒死蜱、噻螨酮、柴油、哒螨灵、唑螨酯可能存在负交互抗性(RI<1)。 相似文献
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朱砂叶螨对两种杀螨剂的抗性遗传力及风险评估 总被引：3，自引：3，他引：3
在室内抗性培育的基础上,应用数量遗传学中的域性状分析法研究了朱砂叶螨对甲氰菊酯和阿维菌素两种杀螨剂的抗性现实遗传力,并对两种药剂在不同杀死率下,朱砂叶螨抗性发展的速率进行了预测。结果表明,用甲氰菊酯、阿维菌素分别连续汰选16、18代后,朱砂叶螨对两者的抗性分别为28.61和4.36倍,抗性现实遗传力分别为0.2685和0.1385。在室内选择条件下,杀死率为50％～90％时,要获得10倍抗性,甲氰菊酯需要约13～6代,阿维菌素需要约28～13代。生物源农药阿维菌素的抗性风险明显低于菊酯类药剂甲氰菊酯。试验结果为朱砂叶螨抗性治理提供了理论依据。 相似文献
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为了明确羧酸酯酶(carboxylesterase,CarE)基因、谷胱甘肽S-转移酶(glutathione S-transferases,GST)基因和过氧化氢酶(catalase,CAT)基因与柑橘全爪螨Panonychus citri对双甲脒抗性的关系,通过BLAST检索,从柑橘全爪螨转录组数据库中对这3种代谢酶抗性相关基因进行鉴定,并采用RPKM法对双甲脒抗性品系和敏感品系代谢抗性相关基因进行表达差异分析,对差异较大的基因作定量PCR检测.基因差异性分析发现,抗性品系中有9条CarE基因、12条GST基因及6条CAT基因表达量发生上调,13条CarE基因、12条GST基因和3条CAT基因表达量发生下调;Pc29773nrt、Pcl7807nlg和Unigene31477为上调倍数最高的3个基因,其log2 ratio (RS/SS)分别为12.95、10.81、10.01.定量分析显示,Pc29773 nrt、Pcl7807nlg和Unigene31477的上调倍数分别为3.72、2.03和3.09,Pc29773 nrt和Unigene31477上调显著.研究表明柑橘全爪螨Pc29773nrt和Unigene31477上调与其对双甲脒的抗性相关. 相似文献
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本研究通过生物测定检测了重庆潼南田间朱砂叶螨种群对5种杀虫杀螨剂的抗药性,并测定了主要代谢酶活性及抗性相关基因的表达量。抗性监测结果显示,朱砂叶螨对联苯肼酯和哒螨灵表现为中等水平抗性,对阿维菌素、丁氟螨酯和甲氰菊酯为低水平抗性。代谢酶活性检测结果显示:田间种群的P450和GST活性明显提高,且多个相关基因的表达也显著上调,而CarE的活性没有显著变化。综上所述,重庆潼南田间朱砂叶螨种群已对联苯肼酯、哒螨灵、阿维菌素、丁氟螨酯和甲氰菊酯产生了不同程度的抗药性,P450和GST活性提高和有关基因过表达是抗性产生的主要原因。 相似文献
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He Lin Xue Chuan-hua Wang Jin-jun Li Ming Lu Wen-cai Zhao Zhi-mo 《Pesticide biochemistry and physiology》2009,93(1):47-52
A Tetranychus cinnabarinus strain was collected from Chongqing, China. After 42 generations of selection with abamectin and 20 generations of selection with fenpropathrin in the laboratory, this T. cinnabarinus strain developed 8.7- and 28.7-fold resistance, respectively. Resistance to abamectin in AbR (abamectin resistant strain) and to fenpropathrin in FeR (fenpropathrin resistant strain) was partially suppressed by piperonyl butoxide (PBO), diethyl maleate (DEM) and triphenyl phosphate (TPP), inhibitors of mixed function oxidase (MFO), glutathione S-transferases (GST), and hydrolases, respectively, suggesting that these three enzyme families are important in conferring abamectin and fenpropathrin resistance in T. cinnabarinus. The major resistant mechanism to abamectin was the increasing activities of carboxylesterases (CarE), glutathione-S-transferase (GST) and mixed function oxidase (MFO), and the activity in resistant strain developed 2.7-, 3.4- and 1.4-fold contrasted to that in susceptible strain, respectively. The activity of glutathione-S-transferase (GST) in the FeR strain developed 2.8-fold when compared with the susceptible strain, which meant the resistance to fenpropathrin was related with the activity increase of glutathione-S-transferase (GST) in T. cinnabarinus. The result of the kinetic mensuration of carboxylesterases (CarE) showed that the structure of CarE in the AbR has been changed. 相似文献
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Ya-ning FengJin Yan Wei SunShu Zhao Wen-Cai LuMing Li Lin He 《Pesticide biochemistry and physiology》2011,100(1):70-73
The carmine spider mite Tetranychus cinnabarinus is the most serious of crop mite pests in China. Their ability to rapidly develop resistance to acaricides has caused difficulty in controlling this mite. In this study, the molecular mechanism of acaricide resistance associated with esterase genes TCE1 and TCE2 was investigated in susceptible and acaricide-resistant strains of T. cinnabarinus. The quantitative real-time PCR (qrtPCR) method was adopted to compare the expression level of two esterase genes TCE1 and TCE2 among four different strains (abamectin-resistant, AbR; fenpropathrin-resistant, FeR; omethoate-resistant, OmR and susceptible strains, S) of T. cinnabarinus. The relative expression level of TCE2 was 1.39-2.47 fold in the three resistant strains compared with the S strain. And after inducing with abamectin, fenpropathrin, and omethoate the highest expression level of TCE2 in the S was 1.64-, 2.92- and 2.24-fold compared with the control, respectively, and this difference was found to be significant. However, there was no obvious difference of the mRNA relative expression levels of TCE1 genes among the four strains, and those of TCE1 were not higher than the control throughout the study. Furthermore, the expression modes of TCE1 and TCE2 in AbR and FeR were similar with that in the S after being treated with abamectin and fenpropathrin, respectively. These results indicated that the enhanced expression of esterase gene TCE2 was associated with acaricide-resistance in T.cinnabarinus. 相似文献
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BACKGROUND: The carmine spider mite (CSM), Tetranychus cinnabarinus, is the most harmful mite pest of various crops and vegetable plants. Pyrethroid insecticide fenpropathrin has been used to control insects and mites worldwide, but CSM has developed resistance to this compound. RESULTS: Three synergists together eliminated about 50% resistance against fenpropathrin in the CSM. A point mutation was identified from the sodium channel gene of fenpropathrin‐resistant CSM (FeR) by comparing cDNA sequences between FeR and susceptible (S) sodium channel genes, which caused a phenylalanine (F) to isoleucine (I) change at amino acid 1538 position in IIIS6 of the sodium channel and has been proven to confer strong resistance to pyrethroid in other species. The mRNA expression of the sodium channel gene in the FeR and abamectin‐resistant strain (AbR), which was included as a control, were both relatively lower than in the S. CONCLUSION: These results demonstrate that a mutation (F1538I) is present in the sodium channel gene in FeR of CSM, likely playing an important role in fenpropathrin resistance in T. cinnabarinus, but that decrease in the abundance of sodium channel did not confer this resistance. The F1538I mutation could be used as a molecular marker for detecting kdr resistance in Arachnida populations. Copyright © 2011 Society of Chemical Industry 相似文献
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柑橘全爪螨 Panonychus citri 是一种重要的世界性害螨, 已对多种常用杀螨剂产生了不同程度的抗性?为有效控制该害螨的危害, 本研究于2017年-2018年通过田间小区试验评价了30%乙唑螨腈·螺螨酯SC?30%乙唑螨腈SC?240 g/L螺螨酯SC?1.8%阿维菌素EC及43%联苯肼酯SC对柑橘全爪螨的防治效果及其对柑橘树的安全性?研究结果表明:试验浓度下, 叶面喷施1次上述药剂均对柑橘树安全?30%乙唑螨腈·螺螨酯SC及30%乙唑螨腈SC能有效控制柑橘全爪螨危害, 速效性好, 持效期长达30 d, 药后1~30 d的防治效果分别为76.55%~100%和81.21%~98.30%?240 g/L螺螨酯SC和43%联苯肼酯SC对柑橘全爪螨的控制作用较好, 速效性一般而持效期可达30 d, 药后1~30 d的防治效果分别为69.15%~91.55%和64.63%~88.46%?1.8%阿维菌素EC难以控制柑橘全爪螨危害, 速效性较差, 持效期约15 d; 药后1~30 d对该害螨的防治效果为57.08%~83.39%?综上所述, 在柑橘生产实践中, 为有效控制柑橘全爪螨为害, 应于害螨初发期叶面喷施1次30%乙唑螨腈·螺螨酯SC 75 mg/kg或30%乙唑螨腈SC 100 mg/kg? 相似文献
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Fenpyroximate resistance in Tetranychus urticae (Acari: Tetranychidae): cross-resistance and biochemical resistance mechanisms 总被引：1，自引：0，他引：1
A field colony of the Two-spotted spider mite, Tetranychus urticae (Koch), resistant to fenpyroximate was further selected with fenpyroximate 5SC for 20 generations at a selection pressure of 30-50% mortality (designated as FR-20 strain). Resistance and cross-resistance levels of the FR-20 strain to 18 acaricides were determined using a spray method. The FR-20 strain was extremely resistant to fenpyroximate [resistance ratio (RR) 252]. The strain exhibited extremely strong positive cross-resistance to acrinathrin (RR 196), and high levels of resistance to benzoximate (RR 55) and propargite (RR 64). Moderate levels of cross-resistance (RR 11-40) to abamectin, fenbutatin oxide, fenpropathrin, pyridaben, pyridaben + bifenthrin and tebufenpyrad were observed. The FR-20 strain showed low levels of resistance (RR < 10) to azocyclotin, bromopropylate, chlorfenapyr, chlorfenapyr + bifenthrin, chlorfenapyr + pyridaben, dicofol, fenazaquin and milbemectin. Synergist experiments with different metabolic inhibitors revealed that piperonyl butoxide had the greatest effect on the efficacy of fenpyroximate, followed by iprobenfos and triphenyl phosphate. In a comparative assay with detoxifying enzymes, the FR-20 strain showed 2.5-fold higher activity in p-nitroanisole-O-demethylation, and 2.5- and 2.2-fold higher activities in alpha- and beta-naphthyl acetate hydrolysis, respectively. These results suggested that enhanced activities of both mixed-function oxidases and esterases likely contribute to the fenpyroximate resistance of the FR-20 strain of T urticae. 相似文献
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Lu Qian 《Pesticide biochemistry and physiology》2008,91(3):175-179
Experiments have been carried out to confirm the cross-resistance between abamectin and tebufenozide in Plutella xylostella and demonstrate its mechanism. The results showed that the resistant strain of P. xylostella selected by tebufenozide (RF 99.38) really showed high cross-resistance to abamectin (RF 29.25). When this strain was subjected to resistance decaying treatment, breeding without contacting any insecticides, and abamectin resistance selection for 20 generations, the former resulted in decrease of its resistance to both tebufenozide and abamectin to about one third of the original (RF 35.03 and 11.67, respectively), and the later enhanced its resistance to abamectin dramatically (RF 303.77), but not to tebufenozide(RF 50.04). PBO showed high synergism to abamectin (SR 2.11-12.23), and the synergism ratio positively related to the resistance level among different strains. Enzyme analysis also proved that the activity of cytochrome P450 monooxygenase (MFO) was notable enhanced in the strains resistant to both tebufenozide and abamectin (1.71- to 3.01-fold). Based on discussion, it was concluded that tebufenozide selection could resulted in significant cross-resistance of P. xylostella to abamectin. The major mechanism for the cross-resistance should be the enhancement of MFO activity. For resistance management, tebufenozide and abamectin would not recommend for rotational use. 相似文献
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二点叶螨对氧乐果、甲氰菊酯、四螨嗪及螨嗪菊酯混剂的抗药性 总被引：5，自引：0，他引：5
以兰州吐鲁沟公园金花忍冬植物上采集的二点叶螨为敏感种群,在室内盆栽菜豆苗上饲养繁殖后分别用氧乐果、甲氰菊酯、四螨嗪及螨嗪菊酯(甲氰菊酯 四螨嗪)混剂喷雾处理20代,获得二点叶螨抗氧乐果种群(抗性指数RF=35.84倍)、抗甲氰菊酯种群(RF=479.79倍)、抗四螨嗪种群(RF=67.26倍)以及抗混剂螨嗪菊酯种群(RF=26.75倍)。用生化法测定离体酶活性的结果表明,上述四个抗性种群的形成与体内羧酸酯酶、磷酸酯酶、谷胱甘肽转移酶的活力增加及乙酰胆碱酯酶的活性降低有关。4个抗性种群对常用15种供试药剂交互抗性测定结果表明,氧乐果、甲氰菊酯与联苯菊酯、三氟氯氰菊酯、水胺硫磷、久效磷、氰久合剂有交互抗性,甲氰菊酯还与螨蚧克有交互抗性;四螨嗪与三氯杀螨醇(RF=14.15倍)、齐螨素(RF=10.26倍)有交互抗性;螨嗪菊酯与双甲脒、氧乐菊酯有负交互抗性,RF值分别为0.85、0.71倍。 相似文献