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1.
Juan Majada Celia Martínez-Alonso Isabel Feito Angelo Kidelman Ismael Aranda Ricardo Alía 《New Forests》2011,41(3):399-412
Pinus pinaster Ait. is one of the main forest tree species planted in Spain, Portugal and France. Due to its high economic relevance, there
is considerable interest in developing techniques for vegetative breeding aimed at mass propagation. In this study we present
a mini-propagation protocol in order to define an efficient method to propagate families or clones of P. pinaster. We carried out three experiments using mini-cuttings of 3–5 cm in length with the aim of evaluating the effects of temperature
(4°C vs. 25°C), plant growth regulator (IBA) and shoot age on rooting ability. Percentage of rooted cuttings and morphological
root variables were recorded. The percentage of rooted cuttings per treatment ranged from 68 to 97%. Treatment with IBA significantly
influenced the rooting process at 25°C but not at 4°C. The number of apexes, length, area and volume of roots were all positively
affected by temperature treatment. Shoot age also had a positive effect on rooting capacity of cuttings, with the cuttings
from the youngest shoots (70 days after pruning) having higher rooting percentages, ranging from 84.7 to 98.3%. The use of
juvenile material, good environmental conditions and IBA all benefited the rooting of clonal material, resulting in high rooting
capacity. This study presents an innovative propagation protocol for P. pinaster that can be used as a tool in breeding programs. 相似文献
2.
By propagating lodgepole pine (Pinus contorta) cuttings in vivo, we obtained after 7 growing cycles (ca 3.5 years) in a greenhouse, sufficient number of cuttings from most families to establish clonal progeny tests. Twenty-one full-sib families with approximately 20 clones per family were studied for five years. Years when cuttings were set, families within latitude and clones within families differed significantly in rooting percentages, with the variance components 4.2%, 8.2% and 9.5%, respectively. One way to get a frequent and uniform rooting is to take cuttings from non-leading shoots since they have higher rooting percentage than leading shoots. Neither total length of the cuttings nor length of the primary needles were significantly correlated to rooting percentage. With appropriate management of the ortets and the cuttings during rooting, most clones could be included in a cutting propagation program. 相似文献
3.
《Scandinavian Journal of Forest Research》2012,27(4):313-317
Abstract One-year-old Pinus sylvestris donor plants were top-pruned in early May, allowing cuttings of three types to develop: shoots from adventitious buds near the top of the plant (T), weaker shoots from adventitious buds emerging further down the stem (M) and shoots from normal buds on branches (B). Cuttings from 46 half-sib families were collected at the beginning of August, treated with indole-butyric acid, immediately inserted in containers with porous substrate and put in a greenhouse, where soil was heated and high air humidity was maintained. The rooting frequency, estimated by counting living plants 10 months after insertion, was 54%. The family variance component for rooting was small and non-significant, while the clone-within-family component was strongly significant and accounted for 21% of the total random variance. Type M and type B cuttings showed significantly better rooting ability than type T cuttings. Including only plants with at least four acceptable shoots in the propagation experiment, 61% of the potential donor plants remained, without loss of any family. If a more restricted selection criterion was applied, including only plants with at least eight acceptable shoots, 21% remained, with a loss of 13 families. It was concluded that rooting ability of P. sylvestris can be brought to a level that could justify its inclusion in a breeding programme. The lack of family variation was also favourable in this respect, as no families were lost owing to low rooting response. However, the number of suitable cuttings produced by the donor plants needs to be increased. 相似文献
4.
Tarit Kumar Baul Mohammad Mosharraf Hossain Mohammad Mezbahuddin Mohammed Mohiuddin 《林业研究》2011,22(3):409-416
In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala (Roxb) Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid (IBA) treatments. Significantly better rooting response (p ≤ 0.05) was observed with 0.1% IBA compared to control (0% IBA). The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference (p≤0.05). After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest (100 ± 0.00%) survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly (p≤0.05) among the treatments. Effects of three fertilizer treatments, viz. T0 (no fertilizer), T1 (10g Urea, 20g TSP, 10g MOP dissolved in 1 L water) and T2 (10g Urea, 20g TSP, 10g MOP dissolved in 2 L water) on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher (p≤0.05) under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags. 相似文献
5.
It was the aim of this study to determine if Calliandra calothyrsus could be vegetatively propagated in a low-tech non-mist propagation environment suitable for the rural tropics. In addition,
within and between provenance variation in the rooting ability of C. calothyrsus was determined. Leafy stem cuttings were propagated successfully in non-mist propagators, with 100% rooting success recorded
for some genotypes. The average rooting potential of the best rooting provenance was 76% for San Ramon provenance from Nicaragua.
Cuttings harvested from stockplants grown from seed collected at La Ceiba, Honduras, were also successful with 65% rooting;
while the least successful provenance was Suchitepeques, Guatemala, which did not form adventitious roots. It is concluded
that low-tech vegetative propagation is a viable means of providing planting stock of C. calothyrsus, and that the development of operational scale propagation procedures would be worthwhile. 相似文献
6.
A study to determine the best vegetative propagation protocol was carried out for Dalbergia melanoxylon. Natural regeneration of D. melanoxylon is limited due to poor seed viability and germination. The effects of age of donor plant, indole-3-butyric acid (IBA) treatment
and cutting position on rooting of stem cuttings were investigated. The effect of age of donor plant was significant (P < 0.001) only for percent rooting, position effect was significant for all studied parameters, and IBA treatment at 300 ppm
was insignificant only for percent callusing. Interactive effects of cutting position × age of donor and cutting position × IBA
treatment were significant for percent rooting and root number per cuttings while interactive effect of age × IBA was significant
only for percent rooting. Observed difference in rooting performance could be due to uneven distribution of root promoting
and inhibiting substances with respect to age of donor plant and IBA which intensified root formation. Cuttings from juvenile
donor plant performed better in all rooting parameters than mature donor plant. The highest percentages in rooting were (71.11%)
and (24.42%) for cuttings from juvenile and mature donor plants, respectively. IBA treated cuttings produced higher percentage
rooting, number of roots and root length than untreated cuttings which revealed a strong IBA influence on rooting ability
of stem cuttings in D. melanoxylon. It was concluded that stem cuttings should be taken from juvenile donor plants and basal cutting position should be used
to produce high quality planting stock material for the afforestation and conservation programme of D. melanoxylon. 相似文献
7.
The main objective of our clonal propagation program is the production of high quality timber and faster tree growth. Teak
(Tectona grandis Linn. f.) is an important plantation species in the tropics but the demand of teak timber is increasing globally. Teak planting
stock is still produced from seeds of unselected elite genotypes which may lead to poor stand establishment. The number of
seeds per fruit and their germination ability are also variable and limited by several factors. Therefore an alternative method
i.e., clonal propagation is required for mass multiplication of elite trees. It is an established fact that the clonal propagation
or adventitious root formation in cuttings is markedly affected by several external and internal factors. Considering these,
an experiment was conducted on leafy soft wood shoot cuttings of 3-year-old hedged teak stock plants grown in a hedge garden
to study the effects of branch position and auxin treatment on adventitious root formation. A factorial completely randomized
design was used for experimentation. Coppice shoots were collected from basal, middle and upper positions of the hedge garden;
and mono-nodal leafy cuttings were prepared. These cuttings were treated with different concentrations of IBA and NAA; and
were cultured in a mist chamber for rooting where the relative humidity was maintained at 85 ± 2% with maximum and minimum
day-night temperature at 32 ± 1°C and 26 ± 1°C respectively. After 45 days the cuttings were removed from the rooting medium
and observations were made. It was observed that the rooting parameters viz., percent rooting and percent sprouting, mean
number of leaves, mean number of shoots and length, and mean number of roots and length per cutting were significantly influenced
by the branch position and auxin treatment. Treatment with 4000 ppm IBA increased percent rooting and percent sprouting whereas
NAA suppressed it. Treatment with 4000 ppm IBA also increased the mean number of leaves and shoots, and the length, and the
mean number of roots per cutting. The highest mean number of roots and length were observed in cuttings taken from the branch
emerging at the upper position followed by branches of middle and lower position. Cuttings of branches at a middle position
showed the highest values of percent rooting, percent sprouting, mean number of leaves and shoots and mean shoot length per
cutting. Furthermore, the interactive effect of branch position and auxin treatment on rooting and sprouting response was
also found to be significant. Overall, the findings of this investigation suggest that middle branch position in hedge plants
and 4000 ppm IBA can be made to induce maximum rooting and to produce high quality planting stock material for clonal forestry
program of teak. 相似文献
8.
9.
罗田垂枝杉不同材料扦插试验研究 总被引：1，自引：1，他引：0
为探索罗田垂枝杉的无性繁殖,以大树侧枝和基部萌条及采穗圃穗条为试验材料进行扦插,分析不同材料、不同系号及不同激素处理生根差异。结果表明:20 a以上的大树Ⅱ级侧枝的扦插生根率较低,为48.11%,而Ⅰ级侧枝的扦插生根率可达65%以上;萌条扦插生根率(88.86%)要远高于优树侧枝扦插生根率(48.01%),两者差异达到极显著水平(p0.01),黄化未木质化穗条扦插生根率仅为6.67%,远低于其他萌条86.94%~91.67%的生根水平,不同系号间萌条生根率从51.57%~98.77%不等;激素处理的采穗圃穗条生根率都在91.57%以上,粉剂1处理后生根数量最多,与其余三种处理差异达到显著水平。可见侧枝扦插要显著差于萌条扦插,而激素处理可以有效提高扦插生根率。 相似文献
10.
Welander M 《Tree physiology》1995,15(1):11-18
Differences in rooting ability of birch (Betula pubescens J.F. Ehrh.) cuttings were observed as a result of differences in genotype and physiology of the stock plants. The uniformity in response among cuttings from micropropagated plants compared with cuttings from seed plants confirmed the advantage of using micropropagated plants to study environmental effects. Shoot morphology of the seed stock plants was influenced by both photoperiod and thermoperiod. A day/night temperature of 15/25 degrees C reduced stem elongation compared with a day/night temperature of 25/15 degrees C regardless of photoperiod, and a continuous light regime resulted in more shoots per plant in both temperature regimes than a 16-h photoperiod. A reduction in the supply of macronutrients did not influence shoot morphology, but increased rooting substantially and seemed to override the effects of environmental factors. In cuttings of seed plants, the highest rooting percentage and number of roots were obtained in a 16-h photoperiod with a day/night temperature of 15/25 degrees C. In micropropagated stock plants, there was a positive correlation between shoot length and number of leaves per shoot and topographical distribution of light within the plants, but there was no correlation between these parameters and rooting ability of the cuttings. A rooting temperature of 16 degrees C delayed the rate of root production compared with the rate at higher temperatures, but the final rooting percentage was the same over the range from 16 to 28 degrees C. Root branching increased with temperature. At all temperatures, there was a large increase in sucrose content at the base of the cuttings during rooting, whereas the concentration of nontranslocated sugars remained constant. The carbohydrate content at the base of cuttings from micropropagated stock plants was three times higher than at the base of cuttings from seed stock plants, but the higher carbohydrate content was not correlated with a higher rooting potential. 相似文献
11.
Catherine Ky-Dembele Jules Bayala Antoine Kalinganire Fatoumata Tata Traoré Bréhima Koné Alain Olivier 《Southern Forests》2016,78(3):193-199
Pterocarpus santalinoides is a multipurpose tree species, native to Africa and South America. However, excessive pruning for fodder is jeopardising its seed production leading to poor regeneration in the Sahel region. Efficient vegetative propagation techniques may overcome this concern. To address this issue, four experiments were conducted to determine the effects of substrate (Experiment 1), cutting type (Experiments 2 and 3), genotype (Experiment 4) and auxins (Experiments 1, 2, and 4) on rooting ability in a non-mist propagating unit in Mali. Among the four tested substrates, no significant differences were found in rooting parameters, including the percentage of rooted cuttings, the number of roots and secondary roots per rooted cutting, and the length of the longest root per rooted cutting. Semi-hardwood cuttings rooted significantly better than softwood cuttings with a higher percentage of rooted cuttings (31.7?±?6.5% vs. 9.2?±?2.7%), greater number of roots (8.2?±?4.8 vs 1.5?±?0.7), greater number of secondary roots (16.7?±?4.2 vs 4.6?±?1.8) and longer root length (17.8?±?12.0?cm vs 2.3?±?1.0?cm) per rooted cutting. In addition, one-node cuttings cut below the second and third nodes, respectively, had the highest rooting success (88% and 90%, respectively) followed by cuttings cut below the fourth node (68%) and the first node (62%). Significant differences in the percentage of rooted cuttings were found among 12 randomly selected genotypes with rooting varying from 2% to 75%.The results also showed that auxin application, especially IBA, had a significant effect on rooting success, the number of roots and secondary roots per rooted cutting. Single-node cuttings, and cuttings of 10?cm length with multiple softwood and semi-hardwood nodes, can be used with or without auxin treatment for successful clonal propagation of P. santalinoides. The results of the present study indicated that P. santalinoides is amenable to clonal propagation using low-cost propagators and sand-based rooting media. 相似文献
12.
This study investigates the optimal conditions for the vegetative propagation of the multipurpose leguminous shrub Colutea istria from leafy stem cuttings. The conditions examined included 1) the origin of the cutting (apical, medial or basal), 2) indole-3-butyric
acid (IBA) treatment (0, 50, 200 or 400 mg/l), 3) the relative humidity of the propagation environment (high or low), 4) wounding
base of cutting, and 5) the season in which the cutting was taken. The rooting ability of the cuttings was significantly influenced
by all these factors. Winter cuttings obtained from the middle and base of the branch showed a greater rooting capacity than
those harvested from the apical area, while autumn cuttings harvested from the middle and apical areas of the branch showed
a greater rooting capacity than those harvested from the base. Winter cuttings showed the best rooting capacity than autumn
cuttings. IBA treatment (200 mg/l), high humidity (98-100%), and wounding increased the rooting capacity of the cuttings. The sprout
of new leaves was higher in basal cuttings, while IBA and wounding also significantly enhanced the root number of rooted cuttings.
A method for vegetatively propagating C. istria plants is given. 相似文献
13.
杂种松扦插繁殖试验初报 总被引：9，自引：4，他引：9
通过近３年的杂种松扦插繁殖试验结果表明,选用黄泥心土基质、穗条采下后浸于５０ｍｇ／ｋｇ的台化牌植物生根促进剂溶液或１ｇ／ｋｇ多菌灵溶液各０．５ｈ或浸于１ｇ／ｋｇ多菌灵溶液０．５ｈ后蘸黄泥浆扦插等处理、在冬春季节光照充足的圃地、穗条长６～１４．５ｃｍ、顶端次生叶长５．５～１３ｃｍ等条件下扦插生根率高;并且在穗条、管理措施、扦插季节等条件合适的情况下,部分家系的扦插生根率较稳定,可达到７５％,最高达１００％。大规模扦插试验平均生根率达９１％。 相似文献
14.
《Scandinavian Journal of Forest Research》2012,27(1-4):27-36
Leader shoots from juvenile succulent Norway spruce seedlings were used as cuttings 11 weeks after sowing. The seedlings were grown for 6 additional weeks and then apically dominant and lateral shoots were also used as cuttings. These three cutting categories, leaders, dominants and laterals, rooted 99.4, 99.0 and 97.5 % respectively and performed differently with respect to rooting development, number of roots per cutting and response to IBA and PIBA treatments (synthetic auxins). A large individual variation in number of roots per cutting, which auxin treatments did not reduce, was present within each cutting categoty. Laterals from the three different families included in the study showed a different variation than did leaders and dominants. Generally, the juvenile succulent cuttings were easy to root. Seedlings grown at a rather high light intensity (62 W/m2) had the potential to give good rooting even without hormonal treatments. 相似文献
15.
R. L. Jinks 《New Forests》1995,10(2):183-195
The effect of propagation environment on the rooting of field collected leafy cuttings of ash (Fraxinus excelsior, sycamore (Acer pseudoplatanus) and sweet chestnut (Castanea sativa) was investigated in two experiments. In the first experiment, rooting of all three species in mist enclosed inside a polythene tent was greater than 50%, while rooting of ash cuttings was reduced from 63% in enclosed mist to only 16% in open mist. Rooting in open mist, enclosed mist, and contact polythene was studied in a second experiment. Sycamore cuttings rooted equally well in the two mist systems with an average rooting of 78%. In contrast cuttings of both ash and sweet chestnut rooted most under the enclosed mist system, at 64 and 46% respectively. Rooting of ash cuttings was again depressed in the open mist system where less than 30% of cuttings rooted. This reduction was related to an increase in the percentage of cuttings which remained alive without rooting, while showing proliferation of callus at the cutting base. The percentage of sweet chestnut cuttings which callused without rooting was also much greater in the open mist system than in enclosed mist or contact polythene. 相似文献
16.
The effect of auxins (IAA or IBA at 100,200,500 mg L 1) on rooting and sprouting parameters differed significantly (p < 0.05).Rooting and sprouting percentages were higher in some treatments while other parameters were significantly higher in other treatments.The results of the present study revealed that rooting was significantly (p < 0.05) affected by the length of cuttings,as well as the position of shoots and type of cuttings.On average,86.0 per cent rooting was observed in the 30-cm long cuttings compared to the other two lengths of cuttings,i.e.,20 and 25 cm,irrespective of any auxin treatment.In a control set,without any auxin treatment,a maximum of 60.0% rooting was recorded in the 30-cm long cuttings and a minimum of 40.0% of the cuttings rooted in the 20-cm long cuttings.Similarly,cuttings collected from the lower and upper portions of shoots resulted in 72.5 and 52.5 per cent rooting respectively,irrespective of auxin treatments.Maximum rooting was observed in the lower portion (40.0%) of cutting compared to the upper portion (30.0%) in the control set without auxin treatment.Leafless branches produced maximum (72.0%) rooting compared to the leafy cuttings (35.0%),irrespective of the auxin treatment.The leafy cuttings produces a minimum of 15.0% sprouting and rooting in the control set. 相似文献
17.
Effects of genotype, collection date, auxin treatment, crown position and artificial chilling on rooting and subsequent vegetative growth of Cupressus sempervirens L. softwood cuttings taken from 13-year-old plants were evaluated. Three different clones (318, 296 and 22) previously selected for resistance to cypress cortical cancer caused by Seiridium cardinale were used. In all these clones, both the IBA treated and the untreated cuttings showed seasonal periodicity of rooting. Untreated cuttings rooted best when collected in April (up to 49% for the best clone, i.e. 296) and rooted very poorly in July and October. Auxin treatments appeared unable to overcome this periodicity, as the cuttings taken in July and October did not respond to IBA stimulation. However, in January and April, IBA treatments enhanced adventitious root formation of poorly rooting clones (318 and 22), as well as of clone 296, which performed best (89%) when cuttings were treated in April with 1.5% IBA. Cuttings of each clone collected in the lower third of the crown of the donor trees showed a significantly higher rooting percentage, when compared with those from the upper third. A 4-week post-severance chilling treatment (4°C) significantly enhanced the rooting capacity of cuttings taken in January, while a longer chilling treatment (8 weeks) had a negative effect. The subsequent growth of the rooted cuttings was not affected by either the crown position of the severed cuttings, or the genotype. 相似文献
18.
The influence of seasonal variation, indole-3-butyric acid (IBA) and type of cuttings was examined on induction and growth
of adventitious roots in Bambusa nutans Wall. and Bambusa tulda Roxb. Singlenode culm and culm-branch cuttings from the mature culms were provided with immersion treatment for 24 h of either
water (control) or 2 mM IBA in four different seasons, i.e., spring (mid February), summer (mid May), rainy (mid July), and
winter (mid November) and maintained for two months in the mist chamber at the relative humidity of (70±5)% and the temperature
of (30±2)°C. In B. nutans, adventitious rooting occurred in both types of cuttings in all the seasons with the best rooting in the summer season i.e.,
May (88% in culm cuttings) and the least in winter. On the contrary, adventitious rooting was recorded only in culm cuttings
in spring and summer season in B. tulda. IBA treatment significantly enhanced rooting, root number and root length; registering 14 to 17 times improvement over control
in the best rooting season. Three factor-interactions (season × cutting type × IBA treatment) were significant for rooting
in B. nutans and all characteristics, except sprouting in B. tulda. Thus, single-node culm and culm-branch cuttings in B. nutans and culm cuttings in B. tulda treated with 2 mM IBA during spring (February) to summer (May) season are recommended for their clonal multiplication. 相似文献
19.
Øystein Johnsen 《Forest Ecology and Management》1985,11(4):271-282
The results of bulk propagation of Norway spruce for one year are presented. The number of cuttings produced per seedling ranged from 21 to 38 between different families, with a large within-family variation. No clear relationship between family field performance and production of cuttings could be seen. Established cuttings produced more propagules per plant than seedlings in the first propagation. Misting gave about 92% rooting, with no significant differences between different rooting media used. A bulk propagation programme is proposed with a potential production of about 1000–1500 plants per germinated seed within 3 years of sowing. 相似文献
20.
大规模生产杉木改良苗木的方法 总被引：3，自引：1，他引：3
1986年李明鹤把林木改良区分为个体改良与群体改良,并在此基础上提出了采用无性系选择法,分两步迅速实现杉木良种化的方案。从1986年起,经三年的实验,现已完成了第一步,并研究出了建立采穗圃的新技术,它有助于使我国杉木造林迅速实现良种化。现将研究的结果报道如下。 相似文献