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A 27‐year‐old Thoroughbred gelding was examined for a right nasal mass visible inside the right nares. Airflow through the right nostril was absent. Endoscopy and radiography revealed the mass to occupy the entire right nasal passage. Nasal biopsies were inconclusive, so en bloc resection was performed. A diagnosis of an incompletely resected osteoblastic osteosarcoma was made. Endoscopic biopsies performed 4 weeks post surgery revealed osteosarcoma cells present in the caudal right nasal cavity. Metastatic disease was not present in mandibular lymph node aspirates or on thoracic radiographs. The right nasal passage was irradiated with 12 treatments over the course of 4 weeks. Comfort and quality of life were excellent during treatment and no adverse side effects were noted. Endoscopy and follow‐up biopsies at 1, 2, 4, 12 and 14 months post radiation therapy have not found any evidence of regrowth of the osteosarcoma.  相似文献   

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An 18-year-old African elephant was determined to have a nonrepairable crack in its left tusk. Treatment included extraction of the tusk, using rotational and extractional forces, and administration of antibiotics, followed by 1 year of flushing the opened tusk cavity with warm tap water. Two years after surgery, the elephant was healthy, and the tusk cavity was 80% filled with normal tissue.  相似文献   

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Objective: To report surgical planning, technique, and outcome of stabilization surgery in an adult dog with occipitoatlantoaxial malformation (OAAM). Study Design: Clinical report. Animal: A 19‐month‐old, 25.5 kg, male castrated, Shiba Inu. Methods: Radiographic and magnetic resonance imaging were used to identify and characterize OAAM. Using a ventral approach to the cranial cervical region 2 cortical bone screws were inserted from the axis into the malformed atlas and occiput. Results: Ambulation was conserved postoperatively. Within 4 weeks, neurologic examination was mostly normal except for decreased proprioception in the right pelvic limb. At 9 months, the dog retained an extended neck posture, but had no neurologic abnormalities. Conclusion: OAAM should be considered as a differential diagnosis in an adult dog with cervical myelopathy. Surgical fixation with cortical bone screws using a ventral approach can be successful.  相似文献   

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An open radial fracture in an adult horse (450 kg) was repaired by internal fixation, using two 18-hole 4.5-mm broad dynamic compression plates and 5.5- and 4.5-mm bone screws. The fracture healed completely, but when evaluated 9 months after surgery, the horse was lame on the fractured limb at a trot. Local infiltration of anesthesia along the distal half of the bone plates greatly ameliorated the lameness, suggesting that the plates were irritating the soft tissues and extensor tendons along the cranial and lateral aspects of the antebrachium. Both bone plates were removed simultaneously with no complications, and the horse became sound.  相似文献   

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This case report describes the use synthetic bone graft particulate and 24% EDTA gel to treat an infrabony defect adjacent to the mandibular right first molar tooth in an American Eskimo dog. Postoperative examination 33-months following surgery showed osseous integration at the infrabony defect and restoration of the periodontal ligament space with a small refractory periodontal pocket.  相似文献   

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Ectopic teeth occur because of failure of the first branchial cleft to close during development and are found mostly in young horses. Such dentigerous cysts are often located at the base of the ear, forming a notable swelling with a fistula, as it was the case with the two year old Iceland mare ?Runa?. In order to confirm the diagnosis, x-ray images were taken, which is also necessary to locate the ectopic tooth correctly. While operating, the whole cystic membrane should be removed and it is important to prevent adjacent nerves and blood vessels from damage. Prognosis for complete healing after removing an ectopic tooth is excellent.  相似文献   

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A fully virulent field Anaplasma marginale isolate, passaged to a splenectomised white tailed deer, lost virulence for cattle after 18 months in the non-bovine host. This attenuated A. marginale produced a mild response in four 18-month-old Holstein steers characterised by an average 2.4% parasitaemia, a 13% drop in PCV and a positive serological response. All vaccinated steers were immune to challenge with a fully virulent, heterologous A. marginale when compared with non-vaccinated controls.  相似文献   

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An enteric infection in cockatoos associated with a 30 nm diameter enterovirus-like agent seen in faeces and intestinal epithelial cells is described. The disease is characterised by intractable, profuse, mucoid diarrhoea, weight loss, dehydration and death. Lesions in the intestine consist of villous atrophy, villous fusion, enterocyte hyperplasia and, in some cases, chronic inflammation. Affected birds so far examined have concurrent psittacine beak and feather disease.  相似文献   

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Sheep red blood cells were fractionated by ion exchange and gel filtration chromatography to yield glutathione peroxidase approximately 99% pure. An antiserum against glutathione peroxidase was raised in the rabbit. The antiserum has been shown to cross-react with both bovine and human glutathione peroxidase by double diffusion.An enzyme linked immunosorbent assay has been developed for glutathione peroxidase which detected 6.15×10–5 IU of the enzyme. The antiserum has also been shown to be effective in the detection of glutathione peroxidase immobolised on strips of nitrocellulose, subsequent to sodium dodecyl sulphate polyacrylamide gel electrophoresis, by second antibody conjugate. Avidin-biotin was also used to detect nitrocellulose immobolised enzyme. These techniques provide an alternative highly sensitive and specific means of assaying glutathione peroxidase which is not dependent on the lability of enzymatic activity nor the chemical specificity of the assay.  相似文献   

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Sheep red blood cells were fractionated by ion exchange and gel filtration chromatography to yield glutathione peroxidase approximately 99% pure. An antiserum against glutathione peroxidase was raised in the rabbit. The antiserum has been shown to cross-react with both bovine and human glutathione peroxidase by double diffusion. An enzyme linked immunosorbent assay has been developed for glutathione peroxidase which detected 6.15 X 10(-5) IU of the enzyme. The antiserum has also been shown to be effective in the detection of glutathione peroxidase immobolised on strips of nitrocellulose, subsequent to sodium dodecyl sulphate polyacrylamide gel electrophoresis, by second antibody conjugate. Avidin-biotin was also used to detect nitrocellulose immobolised enzyme. These techniques provide an alternative highly sensitive and specific means of assaying glutathione peroxidase which is not dependent on the lability of enzymatic activity nor the chemical specificity of the assay.  相似文献   

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End of an era     
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Invasive species control requires understanding the mechanisms behind their establishment and their interactions with other species. One potential ecosystem alteration influencing the establishment and spread of invasive species is anthropogenic nitrogen enrichment, from sources like introduced or invasive nitrogen (N)-fixing legumes, which can alter competition between native, non-native, and invasive plants. Kentucky bluegrass (Poa pratensis) and N-fixing yellow sweet clover (Melilotus officinalis) are exotic to the Great Plains and are currently invading and degrading native rangelands by altering ecosystem processes and displacing native plants. Therefore, we investigated how N enrichment from yellow sweet clover affects the aboveground biomass production of Kentucky bluegrass and western wheatgrass (Pascopyrum smithii), a native cool-season grass, the ranges of which overlap in the northern Great Plains. In a controlled greenhouse environment, we conditioned experimental pots by growing yellow sweet clover and terminating each plant after 8 wk. Conditioned soils contained ≈ 340% more plant-available N than untreated soils 2 wk after yellow sweet clover death. We then grew Kentucky bluegrass and western wheatgrass transplant seedlings in interspecific and intraspecific pairs in pots conditioned either with or without yellow sweet clover for 12 wk. Aboveground biomass production of both Kentucky bluegrass and western wheatgrass grown in interspecific and intraspecific pairs increased in conditioned soils. However, when grown together in conditioned pots, the increase in Kentucky bluegrass biomass relative to untreated pots (520%) was double that of the increase in western wheatgrass biomass (260%). Our results reveal that Kentucky bluegrass can use increased soil N to produce proportionally more aboveground biomass than western wheatgrass, a native grass competitor. Thus, our results suggest yellow sweet clover and other sources of N enrichment may facilitate the invasion of Kentucky bluegrass.  相似文献   

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Composting of poultry carcasses represents an alternative method for disposal in case of an outbreak of an epizootic disease. Two composting experiments, each with a different construction of the compost pile, were carried out in a stable. In the first experiment two layers of turkey carcasses were formed. This compost pile covered with straw was directly built on the ground. In the second experiment no layers of carcasses were formed, and it was assembled on straw bales covered with plastic foil. One part of this compost pile was covered with straw, the other one was additionally covered with plastic foil. In the first experiment in the upper layers of the compost pile temperatures of up to 54.9 degrees C were reached and the decomposition of carcasses was very advanced with no soft tissues remaining after 30 days. In contrast temperatures of only 45.2 degrees C were reached in the lower layers and decomposition was far less advanced. This difference in decomposition was most likely caused by the temperature difference observed. In the second experiment the near complete decomposition seen in the upper layers of the compost pile at the first trial, was not achieved. Decomposition was more advanced in the straw covered part of this compost pile than in the part covered with straw and plastic foil. On the other hand, higher temperatures of up to 48.4 degrees C were measured in the lower layers of this compost pile most likely as a result of the increased heat insulation in particular to the ground.  相似文献   

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Summary An enzyme linked immunosorbent assay (ELISA) test and the classic agar gel immunodiffusion (AGID) test were used as diagnostic methods in the eradication of a focus of equine infectious anaemia from a herd of 86 horses. The ELISA test proved to be more sensitive, detecting positive animals earlier than the AGID test. A group of 16 animals positive only by ELISA also became positive to the AGID when retested one month later, except for 2 animals which showed clinical signs of the disease and died before retesting.
El Uso De Una Prueba Elisa En La Erradicacion De Un Foco De Anemia Infecciosa Equina
Resumen La prubla ELISA (Enzyme Linked Immunosorbent Assay) y la pruebla clásico de imunodifusión en gel agar (IDGA) fueron utilizados como métodos de diagnóstico para eliminar un foco de Anemia Infecciosa Equina. La pruebla de ELISA fue más sensible, detectando los animales positivos antes que la pruebla de IDGA. Un grupo de animales positivos, que fueron detectados solamente por la pruebla de ELISA, fueron también detectados por la pruebla de IDGA en el reteste después de treinta dias. De los 16 animales de ese grupo, solamente 2 mostrarom signos clinicos de la enfermedad y murieron antes de la realización del reteste.

L'utilisation Du Test Elisa Dans L'eradication D'un Foyer D'anemie Infectieuse Equine
Résumé Un test d'ELISA (Enzyme Linked Immunosorbent Assay) et un test cassique d'immunodiffusion en gel d'agar (IDGA) ont été utilisés comme méthodes de diagnostic de l'erradication d'un foyer d'Anémie Infectiuse Equine. Le test d'ELISA a été le plus sensible detectant animaux positifs plus tot que le test d'IDGA. Un groupe d'animaux positifs qui ont été detectés seulement par le test d'ELISA ont aussi été detectés par le test d'IDGA lors d'un nouveau test après trente jours. De ce groupe constituté par seize animaux, seulement deux ont montré des signes cliniques de la maladie et sort morts avant la réalisation du nouveau test.
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