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1.
Eddy covariance measurements and estimates of biomass net primary production (NPP) in combination with soil carbon turnover modelled by the Roth-C model were used to assess the ecosystem carbon balance of an agricultural ecosystem in Thuringia, Germany, growing winter wheat in 2001. The eddy CO2 flux measurements indicate an annual net ecosystem exchange (NEE) uptake in the range from −185 to −245 g C m−2 per year. Main data analysis uncertainty in the annual NEE arises from night-time u1 screening, other effects (e.g. coordinate rotation scheme) have only a small influence on the annual NEE estimate. In agricultural ecosystems the fate of the carbon removed during harvest plays a role in the net biome production (NBP) of the ecosystem, where NBP is given by net ecosystem production (NEP=−NEE) minus non-respiratory losses of the ecosystem (e.g. harvest). Taking account of the carbon removed by the wheat harvest (290 g C m−2), the agricultural field becomes a source of carbon with a NBP in the order of −45 to −105 g C m−2 per year. Annual carbon balance modelled with the Roth-C model also indicated that the ecosystem was a source for carbon (NBP −25 to −55 g C m−2 per year). Based on the modelling most of carbon respired resulted from changes in the litter and fast soil organic matter pool. Also, the crop and management history, particularly the C input to soil in the previous year, significantly affect next year’s CO2 exchange.  相似文献   

2.
After reforesting pasture land, it is often observed that soil carbon stocks decrease. The present work reports findings from a site near Canberra, Australia, where a pine forest (Pinus radiata) was planted onto a former unimproved pasture site. We report a number of detailed observations seeking to understand the basis of the decline in soil C stocks. This is supported by simulations using the whole-ecosystem carbon and nitrogen cycling model CenW 3.1. The model indicated that over the first 18 years after forest establishment, the site lost about 5.5 t C ha?1 and 588 kgN ha?1 from the soil. The C:N ratio of soil organic matter did not change in a systematic manner over the observational period. Carbon and nitrogen stocks contained in the biomass of the 18-year old pine stand exceeded that of the pasture by 88 t C ha?1 and 393 kgN ha?1. An additional 6.1 t C ha?1 and 110 kgN ha?1 accumulated in above-ground litter. These changes, together with the vertical distribution of carbon and nitrogen in the soil, agreed well with the observation at the site. It was assumed that over 18 years, there was also a loss of 86 kgN ha?1 from the ecosystem because of normal gaseous losses during nitrogen turn-over and a small amount of nitrogen leaching. Those losses could not be replenished in the pine system without symbiotic biological nitrogen fixation, and there were no fertiliser additions. A simple mass balance approach indicated that the amount of nitrogen accumulating in plant biomass and the litter layer plus the assumed nitrogen loss from the site matched the amount of nitrogen lost from the soil organic nitrogen pool. This reduction in soil nitrogen, together with an unchanged C:N ratio, provided a simple and internally consistent explanation for the observed reduction of soil carbon after reforestation. It supports the general notion that trends in soil carbon upon land-use change can often be controlled by the possible fates of available soil nitrogen.  相似文献   

3.
We studied a semi-natural forest in Northern Italy that was set aside more than 50 years ago, in order to better understand the soil carbon cycle and in particular the partitioning of soil respiration between autotrophic and heterotrophic respiration. Here we report on soil organic carbon, root density, and estimates of annual fluxes of soil CO2 as measured with a mobile chamber system at 16 permanent collars about monthly during the course of a year. We partitioned between autotrophic and heterotrophic respiration by the indirect regression method, which enabled us to obtain the seasonal pattern of single components.The soil pool of organic carbon, with 15.8 (±4.5) kg m?2, was very high over the entire depth of 45 cm. The annual respiration rates ranged from 0.6 to 6.9 μmol CO2 m?2 s?1 with an average value of 3.4 (±2.3) μmol CO2 m?2 s?1, and a cumulative flux of 1.1 kg C m?2 yr?1. The heterotrophic component accounted for 66% of annual CO2 efflux. Soil temperature largely controlled the heterotrophic respiration (R2 = 0.93), while the autotrophic component followed irradiation, pointing to the role of photosynthesis in modulating the annual course of soil respiration.Most studies on soil respiration partitioning indicate autotrophic root respiration as a first control of the spatial variability of the overall respiration, which originates mainly from the uppermost soil layers. Instead, in our forest the spatial variability of soil respiration was mainly linked to soil carbon, and deeper layers seemed to provide a significant contribution to soil respiration, a feature that may be typical for an undisturbed, naturally maturing ecosystem with well developed pedobiological processes and high carbon stocks.  相似文献   

4.
The substrate availability for microbial biomass (MB) in soil is crucial for microbial biomass activity. Due to the fast microbial decomposition and the permanent production of easily available substrates in the rooted top soil mainly by plants during photosynthesis, easily available substrates make a very important contribution to many soil processes including soil organic matter turnover, microbial growth and maintenance, aggregate stabilization, CO2 efflux, etc. Naturally occurring concentrations of easily available substances are low, ranging from 0.1 μM in soils free of roots and plant residues to 80 mM in root cells. We investigated the effect of adding 14C-labelled glucose at concentrations spanning the 6 orders of magnitude naturally occurring concentrations on glucose uptake and mineralization by microbial biomass. A positive correlation between the amount of added glucose and its portion mineralized to CO2 was observed: After 22 days, from 26% to 44% of the added 0.0009 to 257 μg glucose C g?1 soil was mineralized. The dependence of glucose mineralization on its amount can be described with two functions. Up to 2.6 μg glucose C g?1 soil (corresponds to 0.78% of initial microbial biomass C), glucose mineralization increased with the slope of 1.8% more mineralized glucose C per 1 μg C added, accompanied by an increasing incorporation of glucose C into MB. An increased spatial contact between micro-organisms and glucose molecules with increasing concentration may be responsible for this fast increase in mineralization rates (at glucose additions <2.6 μg C g?1). At glucose additions higher than 2.6 μg C g?1 soil, however, the increase of the glucose mineralization per 1 μg added glucose was much smaller as at additions below 2.6 μg C g?1 soil and was accompanied by decreasing portions of glucose 14C incorporated into microbial biomass. This supports the hypothesis of decreasing efficiency of glucose utilization by MB in response to increased substrate availability in the range 2.6–257 μg C g?1 (=0.78–78% of microbial biomass C). At low glucose amounts, it was mainly stored in a chloroform-labile microbial pool, but not readily mineralized to CO2. The addition of 257 μg glucose C g?1 soil (0.78 μg C glucose μg?1 C micro-organisms) caused a lag phase in mineralization of 19 h, indicating that glucose mineralization was not limited by the substrate availability but by the amount of MB which is typical for 2nd order kinetics.  相似文献   

5.
《Soil biology & biochemistry》2001,33(7-8):983-996
We investigated the influence of earthworms on the three-dimensional distribution of soil organic carbon (SOC) in a chisel-tilled soil. By burrowing, foraging, and casting at the surface and throughout the soil, anecic earthworms such as Lumbricus terrestris L. may play a major role in regulating the spatial distribution of organic matter resources both at the surface and within the soil. In the fall of 1994, we manipulated ambient earthworm communities, which were without deep burrowing species, by adding 100 earthworm individuals m−2 in spring and fall for 3 years. Overall, the biomass of L. terrestris was increased with earthworm additions and total earthworm biomass declined compared with ambient control treatments. To investigate the spatial variability in soil organic carbon due to this shift in earthworm community structure, we sampled soil on a 28×24 cm grid from the surface to 40 cm in four layers, 10 cm deep. Samples were analyzed for total carbon. We found that additions of anecic earthworms significantly increased average soil organic carbon content from 16.1 to 17.9 g C kg−1 for the 0–10 cm soil, and from 12.4 to 14.7 g kg−1 at 10–20-cm depth, and also changed the spatial distribution of soil organic carbon from uniform to patchy, compared with the ambient treatment.  相似文献   

6.
The potential for microorganisms to affect the quantity and quality of organic and condensed forms of phosphorus (P) in soils was investigated by repeated addition of different carbon sources (glucose, starch, cellulose; 2.5 g C kg?1) with or without inorganic P (50 mg P kg?1) to acid and calcareous soils which were either natural soils or clay–sand mixtures free of organic matter. Forms of P after five amendments and subsequent incubation periods of 5 weeks each were analyzed by 31P solution nuclear magnetic resonance (NMR) spectroscopy, and the microbial community composition was assessed by selective plate counts and fatty acid methyl ester (FAME) analysis. All carbon additions induced a redistribution of P from inorganic to organic and condensed forms, which was only little affected by the addition of inorganic P. Compared to non-carbon-amended controls, the greatest increase (7–38 mg P kg?1) in organic P was observed in the monoester region. In the acid clay–sand mixture, there was a large accumulation of pyrophosphate (101 mg P kg?1) after glucose addition and smaller accumulations (6–25 mg P kg?1) after addition of starch and cellulose. Carbon additions increased the microbial biomass in all cases and except in the natural calcareous soil also the proportion of fungi. Redundancy analysis with Monte Carlo permutation tests revealed that for carbon-amended soils, the microbial community composition was more strongly influenced by soil type than by carbon source. Pyrophosphate was positively related to fungi, and diester P was positively related to soil pH. A large proportion of organic and condensed forms of P may still have been in microbial cells at the time of extraction. We have shown that soil organic P consists of some discrete and simple compounds along with some more complex forms, and that organic P recently synthesized by microbes consists almost exclusively of and thus is a likely source for the simple compounds found in natural soils.  相似文献   

7.
《Soil biology & biochemistry》2001,33(4-5):533-551
This study aimed to determine the factors which regulate soil microbial community organisation and function in temperate upland grassland ecosystems. Soil microbial biomass (Cmic), activity (respiration and potential carbon utilisation) and community structure (phospholipid fatty acid (PLFA) analysis, culturing and community level physiological profiles (CLPP) (Biolog®)) were measured across a gradient of three upland grassland types; Festuca–Agrostis–Galium grassland (unimproved grassland, National Vegetation Classification (NVC) — U4a); Festuca–Agrostis–Galium grassland, Holcus–Trifolium sub-community (semi-improved grassland, NVC — U4b); Lolium–Cynosurus grassland (improved grassland, NVC — MG6) at three sites in different biogeographic areas of the UK over a period of 1 year. Variation in Cmic was mainly due to grassland type and site (accounting for 55% variance, v, in the data). Cmic was significantly (P<0.001) high in the unimproved grassland at Torridon (237.4 g C m−2 cf. 81.2 g C m−2 in semi- and 63.8 g C m−2 in improved grasslands) and Sourhope (114.6 g C m−2 cf. in 44.8 g C m−2 semi- and 68.3 g C m−2 in improved grasslands) and semi-improved grassland at Abergwyngregyn (76.0 g C m−2 cf. 41.7 g C m−2 in un- and 58.3 g C m−2 in improved grasslands). Cmic showed little temporal variation (v=3.7%). Soil microbial activity, measured as basal respiration was also mainly affected by grassland type and site (n=32%). In contrast to Cmic, respiration was significantly (P<0.001) high in the improved grassland at Sourhope (263.4 l h−1m−2 cf. 79.6 l h−1m−2 in semi- and 203.9 l h−1m−2 unimproved grasslands) and Abergwyngregyn (198.8 l h−1m−2 cf. 173.7 l h−1m−2 in semi- and 88.2 l h−1m−2 unimproved grasslands). Microbial activity, measured as potential carbon utilisation, agreed with the respiration measurements and was significantly (P<0.001) high in the improved grassland at all three sites (A590 0.14 cf. 0.09 in semi- and 0.07 in unimproved grassland). However, date of sampling also had a significant (P<0.001) impact on C utilisation potential (v=24.7%) with samples from April 1997 having highest activity at all three sites. Variation in microbial community structure was due, predominantly, to grassland type (average v=23.6% for bacterial and fungal numbers and PLFA) and date of sampling (average v=39.7% for bacterial and fungal numbers and PLFA). Numbers of culturable bacteria and bacterial PLFA were significantly (P<0.001) high in the improved grassland at all three sites. Fungal populations were significantly (P<0.01) high in the unimproved grassland at Sourhope and Abergwyngregyn. The results demonstrate a shift in soil microbial community structure from one favouring fungi to one favouring bacteria as grassland improvement increased. Numbers of bacteria and fungi were also significantly (P<0.001) higher in August than any other sampling date. Canonical variate analysis (CVA) of the carbon utilisation data significantly (P<0.05) differentiated microbial communities from the three grassland types, mainly due to greater utilisation of sugars and citric acid in the improved grasslands compared to greater utilisation of carboxylic acids, phenolics and neutral amino acids in the unimproved grasslands, possibly reflecting substrate availability in these grasslands. Differences in Cmic, activity and community structure between grassland types were robust over time. In addition, broad scale measures of microbial growth and activity (Cmic and respiration) showed little temporal variation compared to measures of soil microbial community structure, which varied quantitatively with respect to environmental variables (temperature, moisture) and plant productivity, hence substrate supply.  相似文献   

8.
Earthworms strongly affect soil organic carbon cycling. The aim of this study was to determine whether deep burrowing anecic earthworms enhance carbon storage in soils and decrease C turnover. Earthworm burrow linings were separated into thin cylindrical sections with different distances from the burrow wall to determine gradients from the burrow wall to the surrounding soil. Organic C, total N, radiocarbon (14C) concentration, stable isotope values (δ13C, δ15N) and extracellular enzyme activities were measured in these samples. Anecic earthworms increased C stocks by 270 and 310 g m?2 accumulated in the vertical burrows. C-enrichment of the burrow linings was spatially highly variable within a distance of millimetres around the burrow walls. It was shown that C accumulation in burrows can be fast with C sequestration rates of about 22 g C m?2 yr?1 in the burrow linings, but accumulated C in the burrows may be mineralised fast with turnover times of only 3–5 years. Carbon stocks in earthworm burrows strongly depended on the earthworm activity which maintains continuous C input into the burrows. The enhanced extracellular enzyme activity of fresh casts was not persistent, but was 47% lower in inhabited burrows and 62% lower in abandoned burrows. Enzyme activities followed the C concentrations in the burrows and were not further suppressed due to earthworms. Radiocarbon concentrations and stable isotopes in the burrow linings showed an exponential gradient with the youngest and less degraded organic matter in the innermost part of the burrow wall. Carbon accumulation by anecic earthworm is restricted to distinct burrows with less influence to the surrounding soil. Contrary to the initial hypothesis, that organic C is stabilised due to earthworms, relaxation time experiments with nuclear magnetic resonance spectroscopy (NMR) did not reveal any enhanced adsorption of C on iron oxides with C stabilising effect. Our results suggest that earthworm activity does not substantially increase subsoil C stocks but burrows serve as fast ways for fresh C transport into deep soil horizons.  相似文献   

9.
Soil of the former lake Texcoco is alkaline saline with pH often >10 and electrolytic conductivity (EC) >70 dS m?1 with rapidly changing water contents. Little is known how fertilizing this area with urea to vegetate the soil would affect emissions of carbon dioxide (CO2) and dynamics of N. Texcoco soil with electrolytic conductivity (EC) 2.3 dS m?1 and pH 8.5 (TEXCOCO A soil), EC 2.0 dS m?1 and pH 9.0 (TEXCOCO B soil) and 200 dS m?1 and pH 11.2 (TEXCOCO C soil) was amended with or without urea and incubated at 40% of water holding capacity (WHC), 60% WHC, 80% WHC and 100% WHC, while emissions of nitrous oxide (N2O) and CO2 and dynamics of ammonium (NH4+), nitrite (NO2?) and nitrate (NO3?) were monitored for 7 days. An agricultural soil served as control (ACOLMAN soil). The emission of CO2 increased in the urea amended soil 1.5 times compared to the unamended soil, it was inhibited in TEXCOCO C soil and was >1.2 larger in soil incubated at 40%, 60% and 80% WHC compared to soil incubated at 100% WHC. The emission of N2O increased in soil added with urea compared to the unamended soil, was similar in TEXCOCO A and B soils, but was <0.2 mg N kg?1 soil day?1 in TEXCOCO C soil and generally larger in soil incubated at 60% and 80% WHC compared to soil incubated at 40% and 100% WHC. The water content of the soil had no significant effect on the mean concentration of NH4+, but addition of urea increased it in all soils. The concentration of NO2? was not affected by the water content and the addition of urea except in TEXCOCO A soil where it increased to values ranging between 20 and 40 mg N kg?1. The concentration of NO3? increased in the ACOLMAN, TEXCOCO A and TEXCOCO B soil amended with urea compared to the unamended soil, but not in the TEXCOCO C soil. It decreased with increased water content, but not in TEXCOCO C soil. It was found that the differences in soil characteristics, i.e. soil organic matter content, pH and EC between the soils had a profound effect on soil processes, but even small changes affected the dynamics of C and N in soil amended with urea.  相似文献   

10.
The increasing frequency of periodic droughts followed by heavy rainfalls is expected for this current century, but little is known about the effects of wetting intensity on the in situ biogenic greenhouse gas (GHG) fluxes of forest soils and soil microbial biomass. To gain new insights into the underlying mechanisms responsible for wetting-induced GHG fluxes in situ, rain simulation field experiments during a natural prolonged drought period were done under a temperate forest in northeast China. The intensity of rainfall-induced CO2 pulses increased from 0.84 to 2.08 g CO2–C m? 2 d? 1 with the intensity of wetting up to ca. 80% water-filled pore space, which coincided with an increase in soil microbial biomass and with a decrease in soil labile organic C following wetting. Methane uptake rates decreased from 1.76 to 0.87 mg CH4–C m? 2 d? 1 with the intensity of wetting. Wetting dry forest floor increased N2O fluxes from 6.2 to 25.9 μg N2O–N m? 2 d? 1, but there was no significant difference between all experimental wetted plots. The rainfall-induced N2O pulses with increasing wetting intensity were opposite to that of the CO2 pulses, showing a maximum response at the lowest wetting intensity. An analysis of the temperature sensitivity of GHG fluxes indicated that temperature had an increased effect on the in situ CO2 flux and CH4 uptake, respectively, under wetted and dry conditions. The global warming potential of GHG fluxes and Q10 value of the temperature response of CO2 fluxes increased linearly with wetting intensity. The results indicate that the rainfall-induced soil CO2 pulse is mainly due to enhanced microbial consumption on substrates and highlight the complex nature of belowground C-cycling responses to climate change in northeast China forests that normally experience periodic droughts followed by heavy rainfalls over the year.  相似文献   

11.
In this study, the soil structure of two soils (Haplic Chernozem and Eutric Fluvisol) of different land uses (forest, meadow, urban and agro-ecosystem – consisted of four crop rotations) in Slovakia was compared. The soil aggregate stability was determined with a dependence on the chemical composition of plant residues. The quantity and quality of the organic matter was assessed through the parameters of the C and N in size fractions of dry-sieved and water-resistant aggregates. The soil structure of the forest ecosystem was evaluated as the best of all of forms of land use. Differences in the soil structure under the grass vegetation of a meadow (natural conditions) and urban ecosystem were also recorded. The agro-ecosystem was characterised by a higher portion (55.95%) of the most valuable (agronomically) water-resistant aggregate size fraction of 0.5–3 mm. Values of the carbon management index showed that the larger water-resistant aggregates were, the greater were the changes in the organic matter (r = ?0.680, P < 0.05). In addition, a smaller content of dry-sieved aggregates of the 3–5 mm size fraction was observed with higher contents of soil organic carbon (SOC) (r = ?0.728, P < 0.05) and labile carbon (CL) (r = ?0.760, P < 0.05); there were also greater changes in the soil organic matter and vice versa, higher contents of SOC (r = 0.744, P < 0.05) and CL (r = 0.806, P < 0.05) greater contents of dry-sieved aggregates of size fraction 0.5–1 mm. The soil structure of agro-ecosystem was superior at a higher content of cellulose (r = ?0.712, P < 0.05) in the plant residues. The higher content of cellulose and hemicellulose in the plant residue of a previous crop was reflected in a smaller CL content in the water-resistant aggregates (r = ?0.984, P < 0.05). A correlation was observed between a high content of lignin in the plant residue and a smaller SOC content in the water-resistant aggregates (r = ?0.967, P < 0.05). Lastly, a higher content of proteins in the plant residues (r = 0.744, P < 0.05) supported a greater content of dry-sieved aggregates of the 0.5–1 mm size fraction.  相似文献   

12.
Tree species have significant effects on the availability and dynamics of soil organic matter. In the present study, the pool sizes of soil dissolved organic matter (DOM), potential mineralizable N (PMN) and bio-available carbon (C) (measured as cumulative CO2 evolution over 63 days) were compared in soils under three coniferous species — 73 year old slash (Pinus elliottii), hoop (Araucaria cunninghamii) and kauri (Agathis robusta) pines. Results have shown that dissolved organic N (DON) in hot water extracts was 1.5–1.7 times lower in soils under slash pine than under hoop and kauri pines, while soil dissolved organic C (DOC) in hot water extracts tended to be higher under slash pine than hoop and kauri pines but this was not statistically significant. This has led to the higher DOC:DON ratio in soils under slash pine (32) than under hoop and kauri pines (17). Soil DOC and DON in 2 M KCl extracts were not significantly different among the three tree species. The DOC:DON ratio (hot water extracts) was positively and significantly correlated with soil C:N (R2 = 0.886, P < 0.01) and surface litter C:N ratios (R2 = 0.768, P < 0.01), indicating that DOM was mainly derived from litter materials and soil organic matter through dissolution and decomposition. Soil pH was lower under slash pine (4.5) than under hoop (6.0) and kauri (6.2) pines, and negatively correlated with soil total C, C:N ratio, DOC and DOC:DON ratio (hot water extracts), indicating the soil acidity under slash pine favored the accumulation of soil C. Moreover, the amounts of dissolved inorganic N, PMN and bio-available C were also significantly lower in soils under slash pine than under hoop and kauri pines. It is concluded that changes in the quantity and quality of surface litters and soil pH induced by different tree species largely determined the size and quality of soil DOM, and plantations of hoop and kauri pine trees may be better in maintaining long-term soil N fertility than slash pine plantations.  相似文献   

13.
A 67-day incubation experiment was carried out with a soil initially devoid of any organic matter due to heating, which was amended with sugarcane sucrose (C4-sucrose with a δ13C value of ?10.5‰), inorganic N and an inoculum for recolonisation and subsequently at day 33 with C3-cellulose (δ13C value of ?23.4‰). In this soil, all organic matter is in the microbial biomass or in freshly formed residues, which makes it possible to analyse more clearly the role of microbial residues for decomposition of N-poor substrates. The average δ13C value over the whole incubation period was ?10.7‰ in soil total C in the treatments without C3-cellulose addition. In the CO2 evolved, the δ13C values decreased from ?13.4‰ to ?15.4‰ during incubation. In the microbial biomass, the δ13C values increased from ?11.5‰ to ?10.1‰ at days 33 and 38. At day 67, 36% of the C4-sucrose was left in the treatment without a second amendment. The addition of C3-cellulose resulted in a further 7% decrease, but 4% of the C3-cellulose was lost during the second incubation period. Total microbial biomass C declined from 200 μg g?1 soil at day 5 to 70 μg g?1 soil at day 67. Fungal ergosterol increased to 1.5 μg g?1 soil at day 12 and declined more or less linearly to 0.4 μg g?1 soil at day 67. Bacterial muramic acid declined from a maximum of 35 μg g?1 soil at day 5 to a constant level of around 16 μg g?1 soil. Glucosamine showed a peak value at day 12. Galactosamine remained constant throughout the incubation. The fungal C/bacterial C ratio increased more or less linearly from 0.38 at day 5 to 1.1 at day 67 indicating a shift in the microbial community from bacteria to fungi during the incubation. The addition of C3-cellulose led to a small increase in C3-derived microbial biomass C, but to a strong increase in C4-derived microbial biomass C. At days 45 and 67, the addition of N-free C3-cellulose significantly decreased the C/N ratio of the microbial residues, suggesting that this fraction did not serve as an N-source, but as an energy source.  相似文献   

14.
It is known that carbon (C) amendments increase microbial activity in anoxic soil microcosm studies, however the effects on abundance of total and denitrifier bacterial communities is uncertain. Quantitative PCR was used to target the 16S rRNA gene for the total bacterial community, the nosZ functional gene to reflect a broad denitrifier community, and functional genes from narrow denitrifier communities represented by Pseudomonas mandelii and related species (cnorBP) and Bosea/Bradyrhizobium/Ensifer spp. (cnorBB). Repacked soil cores were amended with varying amounts of glucose and red clover plant tissue (0–1000 mg C kg? 1 of soil) and incubated for 96 h. Carbon amendment significantly increased respiration as measured by cumulative CO2 emissions. Inputs of red clover or glucose at 1000 mg C kg? 1 of soil caused increased abundance in the total bacteria under the conditions used. There was about an approximate 2-fold increase in the abundance of bacteria bearing the nosZ gene, but only in treatments receiving 500 or 1000 mg C kg? 1 of soil of glucose or red clover, respectively. Additions of ≥ 500 mg C kg? 1 soil of red clover and ≥ 250 mg C kg? 1 of glucose increased cnorBP-gene bearing denitrifiers. Changes in abundance of the targeted communities were related to C availability in soil, as indicated by soil respiration, regardless of C source. Applications of C amendments at rates that would occur in agricultural soils not only increase microbial activity, but can also induce changes in abundance of total bacterial and denitrifier communities in studies of anoxic soil microcosms.  相似文献   

15.
Addition of organic manure over thousands of years has resulted in the development of very fertile soils in parts of the Loess Plateau in Northwest China. This region also suffers from serious soil erosion. For that reason, afforestation of arable soils has taken place. The dynamics of soil organic matter in these soils affected by a very specific management and by land use changes is largely unknown. Therefore, we measured C mineralization in a 35-days incubation experiment and analyzed amounts and properties of water-extractable organic carbon (WEOC) in 12 topsoils of this region. The soils differed in land use (arable vs. forest) and in amounts of added organic manure. Afforestation of arable soils resulted in a distinct stabilization of organic C as indicated by the smallest C mineralization (0.48 mg C g−1 C d−1) and the highest C content (2.3%) of the studied soils. In the soils exposed to intensive crop production without regular addition of organic manure we found the largest C mineralization (0.85 mg C g−1 C d−1) and the lowest contents of organic C (0.9%). Addition of organic manure over a time scale of millennia resulted in high organic C contents (1.8%) and small C mineralization (0.55 mg C g−1 C d−1). The content of WEOC reflected differences in C mineralization between the soils quite well and the two variables correlated significantly. Water-extractable organic C decreased during C mineralization from the soil illustrating its mainly labile character. Carbon mineralization from soils was particularly large in soils with small specific UV absorbance of WEOC. We conclude that amounts and properties of WEOC reflected differences in the stability of soil organic C. Both afforestation of arable land and the long-term addition of organic manure may contribute to C accumulation and stabilization in these soils.  相似文献   

16.
Spatial and temporal patterns of soil respiration rates and controlling factors were investigated in three wet arctic tundra systems. In situ summer season carbon dioxide fluxes were measured across a range of micro-topographic positions in tussock tundra, wet sedge tundra, and low-centre polygonal tundra, at two different latitudes on the Taimyr Peninsular, central Siberia. Measurements were carried out by means of a multi-channel gas exchange system operating in continuous-flow mode.Measured soil respiration rates ranged from 0.1 g CO2-C m?2 d?1 to 3.9 g CO2-C m?2 d?1 and rate differences between neighbouring sites in the micro-topography (microsites) were larger than those observed between different tundra systems. Statistical analysis identified position of the water table and soil temperature at shallow depths to be common controls of soil respiration rates across all microsites, with each of these two factors explaining high proportions of the observed variations.Modelling of the response of soil respiration to soil temperature and water table for individual microsites revealed systematic differences in the response to the controlling factors between wet and drier microsites. Wet microsites – with a water table position close to the soil surface during most of the summer – showed large soil respiration rate changes with fluctuations of the water table compared to drier microsites. Wet microsites also showed consistently higher temperature sensitivity and a steeper increase of temperature sensitivity with decreasing temperatures than drier sites. Overall, Q10 values ranged from 1.2 to 3.4. The concept of substrate availability for determining temperature sensitivity is applied to reconcile these systematic differences. The results highlight that soil respiration rates in wet tundra are foremost controlled by water table and only secondarily by soil temperature. Wet sites have a larger potential for changes in soil respiration rates under changing environmental conditions, compared to drier sites.It is concluded that understanding and forecasting gaseous carbon losses from arctic tundra soils and its implication for ecosystem-scale CO2 fluxes and soil organic matter dynamics require good knowledge about temporal and spatial patterns of soil water conditions. The water status of tundra soils can serve as a control on the temperature sensitivity of soil respiration.  相似文献   

17.
Northern peatlands contain substantial reservoirs of carbon (C). Forestry activities endanger the C storages in some of these areas. While the initial impacts of forestry drainage on peatland greenhouse gas (GHG) balance have been studied, the impacts of other silvicultural practices, e.g. logging residue (LR) retention or removal, are not known. We measured the CH4, N2O and CO2 fluxes between peat soil and atmosphere with and without decomposing LR over three (2002–2004) seasons (May–Oct) following clearfelling in a drained peatland forest, along with the mass loss of LR. Seasonal average CO2 efflux from plots with LR (3070 g CO2 m−2 season−1) was twice as high as that from plots without LR (1447 g CO2 m−2 season−1). Less than 40% of this difference was accounted for by the decay of logging residues (530 g CO2 m−2 season−1), so the majority of the increased CO2 efflux was caused by increased soil organic matter decomposition under the LR. Furthermore LR increased soil N2O fluxes over 3-fold (0.70 g N2O m−2 season−1), compared to plots without LR (0.19 g N2O m−2 season−1), while no change in CH4 emissions was observed. Our results indicate that LR retention in clearfelled peatland sites may significantly increase GHG emissions and C release from the soil organic matter C storage. This would make the harvesting of LR for biofuel more beneficial, in the form of avoided emissions. Further investigations of the sources of CO2 under logging residues are, however, needed to confirm this finding.  相似文献   

18.
V.O. Polyakov  R. Lal 《Geoderma》2008,143(1-2):216-222
Soil organic carbon (SOC) is an important component of the global carbon cycle. Its dynamics depends upon various natural and anthropogenic factors including soil erosion. A study on Miamian silty clay loam soil in central Ohio was conducted to investigate the effect of soil erosion on SOC transport and mineralization. Runoff plots 10, 20 and 30 m long on a 7% slope under natural rainfall were used. Total soil loss, evolution of CO2 from the displaced aggregates of various fractions, and total SOC concentrations were determined. It was shown that the primary ways of SOC loss resulted from two processes: 1) mechanical preferential removal of SOC by overland flow and 2) erosion-induced mineralization. Significant amounts of SOC mobilized by erosion at the upper part of the slope during the season (358 kg ha? 1) could be lost to the atmosphere within 100 days (15%) and transported off site (44%). Breakup of initial soil aggregates by erosive forces was responsible for increased CO2 emission. During the initial 20 days of incubation the amount of CO2 released from coarse size sediment fractions (0.282 g C kg? 1 soil d? 1) was 9 times greater than that in fine fractions (0.032 g C kg? 1 soil d? 1) due to the greater initial amount of SOC and its exposure to the environment. Sediment size distribution as well as its residence time on the site was the primary controllers of CO2 loss from eroded soil.  相似文献   

19.
Factors determining C turnover and microbial succession at the small scale are crucial for understanding C cycling in soils. We performed a microcosm experiment to study how soil moisture affects temporal patterns of C turnover in the detritusphere. Four treatments were applied to small soil cores with two different water contents (matric potential of ?0.0063 and ?0.0316 MPa) and with or without addition of 13C labelled rye residues (δ13C=299‰), which were placed on top. Microcosms were sampled after 3, 7, 14, 28, 56 and 84 days and soil cores were separated into layers with increasing distance to the litter. Gradients in soil organic carbon, dissolved organic carbon, extracellular enzyme activity and microbial biomass were detected over a distance of 3 mm from the litter layer. At the end of the incubation, 35.6% of litter C remained on the surface of soils at ?0.0063 MPa, whereas 41.7% remained on soils at ?0.0316 MPa. Most of the lost litter C was mineralised to CO2, with 47.9% and 43.4% at ?0.0063 and ?0.0316 MPa, respectively. In both treatments about 6% were detected as newly formed soil organic carbon. During the initial phase of litter decomposition, bacteria dominated the mineralisation of easily available litter substrates. After 14 days fungi depolymerised more complex litter compounds, thereby producing new soluble substrates, which diffused into the soil. This pattern of differential substrate usage was paralleled by a lag phase of 3 days and a subsequent increase in enzyme activities. Increased soil water content accelerated the transport of soluble substrates, which influenced the temporal patterns of microbial growth and activity. Our results underline the importance of considering the interaction of soil microorganisms and physical processes at the small scale for the understanding of C cycling in soils.  相似文献   

20.
Earthworms play an important role in many soil functions and are affected by soil tillage in agricultural soils. However, effects of tillage on earthworms are often studied without considering species and their interactions with soil properties. Furthermore, many field studies are based on one-time samplings that do not allow for characterisation of temporal variation. The current study monitored the short (up to 53 days) and medium term (up to 4 years) effects of soil tillage on earthworms in conventional and organic farming. Earthworm abundances decreased one and three weeks after mouldboard ploughing in both conventional and organic farming, suggesting direct and indirect mechanisms. However, the medium-term study revealed that earthworm populations in mouldboard ploughing systems recovered by spring. The endogeic species Aporrectodea caliginosa strongly dominated the earthworm community (76%), whereas anecic species remained <1% of all earthworms in all tillage and farming systems over the entire study. In conventional farming, mean total earthworm abundance was not significantly different in reduced tillage (153 m−2) than mouldboard ploughing (MP; 130 m−2). However, reduced tillage in conventional farming significantly increased the epigeic species Lumbricus rubellus from 0.1 m−2 in mouldboard ploughing to 9 m−2 averaged over 4 years. Contrastingly, in organic farming mean total earthworm abundance was 45% lower in reduced tillage (297 m−2) than MP (430 m−2), across all sampling dates over the medium-term study (significant at 3 of 6 sampling dates). Reduced tillage in organic farming decreased A. caliginosa from 304 m−2 in mouldboard ploughing to 169 m−2 averaged over 4 years (significant at all sampling dates). Multivariate analysis revealed clear separation between farming and tillage systems. Earthworm species abundances, soil moisture, and soil organic matter were positively correlated, whereas earthworm abundances and penetration resistance where negatively correlated. Variability demonstrated between sampling dates highlights the importance of multiple samplings in time to ascertain management effects on earthworms. Findings indicate that a reduction in tillage intensity in conventional farming affects earthworms differently than in organic farming. Differing earthworm species or ecological group response to interactions between soil tillage, crop, and organic matter management in conventional and organic farming has implications for management to maximise soil ecosystem functions.  相似文献   

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