共查询到20条相似文献,搜索用时 421 毫秒
1.
The distribution and abundance of a species may be simultaneously influenced by both local-scale habitat features and the
broader patch and landscape contexts in which these populations occur. Different factors may influence patch occupancy (presence–absence)
versus local abundance (number of individuals within patches), and at different scales, and thus ideally both occupancy and
abundance should be investigated, especially in studies that seek to understand the consequences of land management on species
persistence. Our study evaluated the relative influences of variables associated with the local habitat patch, hillside (patch
context), and landscape context on patch occupancy and abundance of the collared lizard (Crotaphytus collaris) within tallgrass prairie managed under different fire and grazing regimes in the northern Flint Hills of Kansas, USA. Using
a multi-model information-theoretic approach that accounted for detection bias, we found that collared lizard abundance and
occupancy was influenced by factors measured at both the local habitat and landscape scales. At a local scale, collared lizard
abundance was greatest on large rock ledges that had lots of crevices, high vegetation complexity, and were located higher
up on the hillslope. At the landscape scale, collared lizard abundance and occupancy were both higher in watersheds that were
burned frequently (1–2 year intervals). Interestingly, grazing only had a significant effect on occupancy and abundance within
less frequently burned (4-year burn interval) watersheds. Our results suggest that, in addition to the obvious habitat needs
of this species (availability of suitable rock habitat), land-management practices have the potential to influence collared
lizard presence and abundance in the grasslands of the Flint Hills. Thus, mapping the availability of suitable habitat is
unlikely to be sufficient for evaluating species distributions and persistence in such cases without consideration of landscape
management and disturbance history. 相似文献
2.
The tallgrass prairie of North America has undergone widespread habitat loss and fragmentation (<4% remains). The Flint Hills
region of Kansas and Oklahoma is the largest tallgrass prairie remaining and therefore provides an opportunity to study the
population genetic structure of grassland species in a relatively contiguous landscape and set a baseline for evaluating changes
when the habitat is fragmented. We adopted a landscape genetics approach to identify how landscape structure affected dispersal,
population genetic structure, and landscape connectivity of the Eastern Yellowbelly Racer (Coluber constrictor flaviventris) across a 13,500-km2 landscape in northeastern Kansas, USA. The racer population had high allelic diversity, high heterozygosity, and was maintaining
migration-drift equilibrium. Autocorrelation between genetic and geographic distance revealed that racers exhibited restricted
dispersal within 3 km, and isolation-by-distance. Significant isolation-by-distance occurred at broad regional scales (>100 km),
but because of sufficient gene flow between locations, we were unable to define discrete subpopulations using Bayesian clustering
analyses. Resistance distance, which considers the permeability of habitats, did not explain significant variation in genetic
distance beyond Euclidean distance alone, suggesting that racers are not currently influenced by landscape composition. In
northeastern Kansas, racers appear to be an abundant and continuously distributed snake that perceives the landscape as well
connected with no cover type currently impeding snake dispersal or gene flow. 相似文献
3.
T. Basaki M. Mardi M. Jafarkhani Kermani S.M. Pirseyedi M.R. Ghaffari A. Haghnazari P. Salehi Shanjani P. Koobaz 《Scientia Horticulturae》2009,120(4):538-543
The genetic diversity among 128 Iranian Rosa persica (R. persica) accessions in the different populations was analyzed. Amplified fragment length polymorphisms (AFLP) technique was used to produce 171 polymorphic fragments. The number of polymorphic loci ranged from 101 to 147 and the polymorphism information content (PIC) varied from 0.289 to 0.073, with an average of 0.16. This shows extreme variability and genetic diversity among the studied R. persica populations. An indirect estimate of the number of migrants per generation (Nm = 0.376) indicated that gene flow was relatively low among populations of the species. Cluster analysis using the UPGMA method grouped all accessions into six clusters. The results did not show relative agreement with the genotypes’ region of origin. Based on an analysis of molecular variance, 48% of the genetic variation of R. persica was within population and 52% was among populations. The present analysis revealed that Iranian R. persica genotypes are highly variable and genetically distinct from their origins. The apparent unique nature of the R. persica genotypes revealed by our results supports the case for the implementation of more intense characterization and conservation strategies, and provides useful information to address breeding programmes and germplasm resource management in Rosa spp. 相似文献
4.
Fine-scale landscape change can alter dispersal patterns of animals, thus influencing connectivity or gene flow within a population.
Furthermore, dispersal patterns of different species may be influenced by the landscape in varying ways. Our research first
aimed to examine whether the spatial genetic structure within populations of closely related bird species differs in response
to the same landscape. Second, we examined whether individual-level movement characteristics are a mechanistic driver of these
differences. We generated a priori predictions of how landscape features will influence dispersal (particularly the response
of individuals to habitat boundaries both natural and human-induced) based on a movement model developed by Fahrig (Funct
Ecol 21:1003–1015, 2007). This model allowed us to predict genetic relatedness patterns in populations of two passerine bird species with different
life-history traits from Queensland, Australia (yellow-throated scrubwren Sericornis citreogularis, a habitat specialist; white-browed scrubwren Sericornis frontalis, a habitat generalist). We quantified our predictions using cost-distance modelling and compared these to observed pairwise
genetic distances (a
r
) between individuals as calculated from microsatellite markers. Mantel tests showed that our a priori models correlated with
genetic distance. Euclidean distance was most closely correlated to genetic distance for the generalist species (r = 0.093, P = 0.002), and landscape models that included the avoidance of unsuitable habitat were best for the specialist species (r = 0.107, P = 0.001). Our study showed that predictable movement characteristics may be the mechanism driving differences in genetic
relatedness patterns within populations of different bird species. 相似文献
5.
Rebecca Lange Tim Diekötter Lisa A. Schiffmann Volkmar Wolters Walter Durka 《Landscape Ecology》2012,27(3):381-392
Unlike rare or specialised species, widespread abundant species have often been neglected when studying effects of habitat
fragmentation. However, recently, it was shown that in the widespread abundant bush cricket Pholidoptera griseoaptera gene flow becomes restricted when the share of suitable habitat dropped below a threshold of 20% at the landscape scale.
Here, using the same highly fragmented landscape, we studied the impact of habitat configuration and matrix quality on genetic
variation and population differentiation of P. griseoaptera at a small spatial scale. We investigated four clusters of three populations that were either disconnected or connected and
had either low quality (arable land) or high quality (grassland) matrix. The number of alleles was significantly lower in
disconnected than in connected clusters, irrespective of matrix quality. Genetic differentiation was equally high in the two
disconnected clusters and in the connected cluster with low quality matrix (G
ST ≥ 0.030; D ≥ 0.082), whereas it was significantly reduced when connected habitats were embedded in a high quality grassland matrix (G
ST = 0.004; D = 0.011). Analyses of least-cost paths showed that grassy landscape elements in fact represent high quality matrix, but that
linear grassy margins are costly for dispersal. The effect of habitat configuration on genetic diversity may be explained
by lower effective population sizes in disconnected habitats. The fact that only the connected populations in high quality
matrix were not differentiated indicates that landscape management should simultaneously consider habitat configuration and
matrix quality to effectively promote small and dispersal-limited species, also at small spatial scales. 相似文献
6.
Kyung-Ho Ma Jae-Gyun Kwag Weiguo Zhao Anupam Dixit Gi-An Lee Haeng-Hoon Kim Ill-Min Chung Nam-Soo Kim Jae-Sun Lee Jae-Jun Ji Tae-San Kim Yong-Jin Park 《Scientia Horticulturae》2009,122(3):355-361
Here we characterized eight novel polymorphic SSR markers, developed from an enriched genomic library of garlic (Allium sativum L.). These SSRs produced a total of 64 alleles across 90 garlic accessions, with an average of 8 alleles per locus. Values for observed (HO) and expected (HE) heterozygosity ranged from 0.16 to 0.77 (mean = 0.44) and from 0.22 to 0.86 (mean = 0.65), respectively. Six loci deviated significantly (P < 0.05) from Hardy–Weinberg equilibrium (HWE). The averages of gene diversity and PIC values were 0.65 and 0.62, respectively. The mean genetic similarity coefficient was 0.4380, indicating that among garlic accessions existed wide genetic variation. Based on 64 alleles obtained by 8 SSRs, a phenogram was constructed to understand the relationships among the 90 accessions. These newly developed SSRs should prove very useful tools for genotypes identification, assessment of genetic diversity and population structure in garlic. 相似文献
7.
Erin L. Koen Jeff Bowman Colin J. Garroway Stephen C. Mills Paul J. Wilson 《Landscape Ecology》2012,27(1):29-43
Landscape heterogeneity can influence animal dispersal by causing a directional bias in dispersal rate, as certain landscape
configurations might promote, impede, or prevent movement and gene flow. In forested landscapes, logging operations often
contribute to heterogeneity that can reduce functional connectivity for some species. American martens (Martes americana) are one such species, as they are considered specialists of late-seral coniferous forests. We assessed marten gene flow
to test the hypothesis that habitat management has maintained landscape connectivity for martens in the managed forests of
Ontario, Canada. We genotyped 653 martens at 12 microsatellite loci, sampled from 29 sites across Ontario. We expected that
if forest management has an effect on marten gene flow, we would see a correlation between effective resistance, estimated
by circuit theory, and genetic distance, estimated by population graphs. Although we found a positive relationship between
effective resistance and genetic distance (Mantel r = 0.249, P < 0.001), marten gene flow was better described by isolation by Euclidean distance (Mantel r = 0.410, P < 0.001). Our results suggest that managed forests in Ontario are well connected for marten and neither impede nor promote
marten gene flow at the provincial scale. 相似文献
8.
Klaus Henle Claudia Andres Detlef Bernhard Annegret Grimm Pavel Stoev Nikolay Tzankov Martin Schlegel 《Landscape Ecology》2017,32(1):131-145
Context
Species show different sensitivity to habitat loss and fragmentation depending on their specialization. Populations of a species at the range margin are generally assumed to be more stenoecious than populations at the core of the distribution and should therefore be more sensitive to habitat fragmentation.Objectives
We evaluated the hypothesis that fragmentation effects species more strongly at the range periphery of their range compared to the core, resulting in lower genetic variability in comparable patch sizes and lower gene flow among populations.Methods
We compared the genetic diversity and structure of five sand lizard (Lacerta agilis) populations at the margin of its range in Bulgaria and of 11 populations at the core of its distribution in Germany. We based the analysis on microsatellites, comprising 15 loci in Bulgaria and 12 in Germany.Results
All diversity indices declined with patch size. For medium-sized patches all diversity indices were lower at the range periphery compared to the core, with two of them being significant. AICc based model selection showed strong support for core/periphery and patch size effects for observed and expected heterozygosity but only a patch size effect for allelic richness. There was no isolation-by-distance and each sampled population was allocated to a separate cluster with high probability for both countries, indicating that all populations are (almost) completely isolated.Conclusion
Our study indicates an increased sensitivity of a species to fragmentation at the periphery compared to the core of its distribution. This differential sensitivity should be accounted for when prioritizing species based on their fragmentation sensitivity in landscape management.9.
Rodney J. Dyer David M. Chan Vicki A. Gardiakos Crystal A. Meadows 《Landscape Ecology》2012,27(2):239-251
The manner by which pollinators move across a landscape and their resulting preferences and/or avoidances of travel through
particular habitat types can have a significant impact on plant population genetic structure and population-level connectivity.
We examined the spatial genetic structure of the understory tree Cornus florida (Cornaceae) adults (N
Adults = 452) and offspring (N
Offspring = 736) across two mating events to determine the extent to which pollen pool genetic covariance is influenced by intervening
forest architecture. Resident adults showed no spatial partitioning but genotypes were positively autocorrelated up to a distance
of 35 m suggesting a pattern of restricted seed dispersal. In the offspring, selfing rates were small (sm = 0.035) whereas both biparental inbreeding (s
b;open canopy = 0.16, s
b;closed canopy = 0.11) and correlated paternity (r
p;open canopy = 0.21, r
p;closed canopy = 0.07) were significantly influenced by primary canopy opening above individual mothers. The spatial distribution of genetic
covariance in pollen pool composition was quantified for each reproductive event using Pollination Graphs, a network method
based upon multivariate conditional genetic covariance. The georeferenced graph topology revealed a significant positive relationship
between genetic covariance and pollinator movement through C. florida canopies, a negative relationship with open primary canopy (e.g., roads under open canopies and fields with no primary canopy),
and no relationship with either conifer or mixed hardwood canopy species cover. These results suggest that both resident genetic
structure within stands and genetic connectivity between sites in C. florida populations are influenced by spatial heterogeneity of mating individuals and quality of intervening canopy cover. 相似文献
10.
Pomegranate (Punica granatum L.) is one of the oldest known edible fruits. It is native to Iran and spread from Iran to other areas. In this study amplified fragment length polymorphism (AFLP) was used to detect intra- and inter-population genetic diversity of pomegranate. A group of 67 accessions belonged to 4 populations from Iran was studied using eight primer combinations. A total of 221 scorable bands were amplified, of which, 118 (54.13%) were polymorphic. Resolving power (Rp) ranged from 5.70 to 9.21, and the average of polymorphism information content (PIC) per primer pair was 0.40. According to Nei's gene diversity and allelic statistics, Isfahan population had a highest genetic diversity (H = 0.3646, I = 0.5327, Ne = 1.6467). Coefficient of gene differentiation between populations (GST) was 0.124, indicated that mainly proportion of genetic variation (87.6%), was within populations and the remaining (12.4%) of the variation was among populations that, also supported by analysis of molecular variance (AMOVA). The gene flow (Nm) varied from 0.969 to 10.404 between pair-wise populations and was 3.504 among all of the populations. The Jaccard similarity coefficient between individuals ranged from 0.26 to 0.88. The UPGMA dendrogram clustered all 67 accessions into 6 groups. In some cases accessions from same region were grouped together but in most cases, there was gene exchange. To study the genetic relationships among populations, a principal coordinate analysis (PCoA) based on Nei's genetic distances was performed. Results of this study showed that AFLP marker can be a useful tool for investigating the genetic diversity of pomegranate genotypes. 相似文献
11.
Paul Arens Theo van der Sluis Wendy P. C. van’t Westende Ben Vosman Claire C. Vos Marinus J. M. Smulders 《Landscape Ecology》2007,22(10):1489-1500
We studied the effects of landscape structure, habitat loss and fragmentation on genetic differentiation of Moor frog populations
in two landscapes in The Netherlands (Drenthe and Noord-Brabant). Microsatellite data of eight loci showed small to moderate
genetic differentiation among populations in both landscapes (F
ST values 0.022 and 0.060, respectively). Both heterozygosity and population differentiation indicate a lower level of gene
flow among populations in Noord-Brabant, where populations were further apart and have experienced a higher degree of fragmentation
for a longer period of time as compared to populations in Drenthe. A significant isolation-by-distance pattern was found in
Drenthe, indicating a limitation in dispersal among populations due to geographic distance. In Noord-Brabant a similar positive
correlation was obtained only after the exclusion of a single long-time isolated population. After randomised exclusion of
populations a significant additional negative effect of roads was found but not of other landscape elements. These results
are discussed in view of improving methodology of assessing the effects of landscape elements on connectivity.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
12.
Random amplified polymorphic DNA (RAPD) analysis was used to characterize genetic diversity of 26 Cyclamen persicum and Cyclamen com accessions. Eighty-four arbitrary primers tested, among which nine primers showed reliable polymorphic banding patterns and yielded 104 polymorphic markers. Jaccard's similarity coefficient among accessions ranged from 0.99 to 0.08. At a similarity of 68%, accessions were divided into three clusters. The cophenetic correlation coefficient between the similarity matrix and the dendrogram was relatively high (r = 0.9) showing the goodness of fit of the dendrogram. The RAPD analysis offered a rapid and reliable tool for the estimation of inter- and intra-specific variability in cyclamens. The wide genetic variation observed for cyclamens within Iran guarantees a promising future of breeding. 相似文献
13.
Understanding interactions between roadways and population structure and movements of wildlife is key to mitigating “road
effects” associated with increasing urbanization of the landscape. Aquatic turtles are a useful focal group because (1) population
persistence is sensitive to mortality of individuals upon roads; (2) turtles frequently move among wetlands and encounter
roads, and (3) turtles are an important component of vertebrate biomass in aquatic ecosystems. From 2005 to 2007, we examined
the effects of urbanization on local- and landscape-scale populations of turtles. To do so, we sampled and marked turtles
in 15 ponds arranged along a steep, urban–rural gradient in central New York State. We captured 494 turtles, representing
327 individuals, the majority of which were common snapping turtles Chelydra serpentina (n = 191) and eastern painted turtles Chrysemys picta picta (n = 122). At the local population (pond) scale, a higher proportion of female snapping turtles in ponds was associated with
lower road densities within 500 m of ponds. The mean size of both species of turtle increased in ponds with a lower density
of roads within 100 m. At the landscape-level, we observed fewer turtles dispersing through urbanized habitat than forested,
and fewer movements through areas with a higher density of roads. Our study suggests that roads alter both local- and landscape-level
turtle populations through a loss of female turtles, and by reducing movement between ponds. By extension, the study targets
key landscape features upon which to focus mitigation efforts. 相似文献
14.
Miltiadis V. Christopoulos Dimos Rouskas Eleni Tsantili Penelope J. Bebeli 《Scientia Horticulturae》2010
Inter-Simple Sequence Repeat (ISSR) markers were used for the assessment of genetic diversity among walnut (Juglans regia L.) selections from Greek native populations in comparison to internationally cultivated walnut genotypes. Similarity coefficient values from 0.13 to 0.93 (with an average of 0.48) were found among the 56 accessions examined, which indicated the presence of a high degree of genetic variability. Most international cultivars were grouped together while most Greek native populations could not be placed into a distinct group. The Greek native population genotypes were found more diverse than the international cultivars. The mean similarity coefficient values for the former and latter were 0.44 and 0.56, respectively. In the cultivar group, two subgroups were distinguished; one consisted of genotypes involving ‘Payne’ and the other ‘Franquette’ in their pedigrees. Some cultivars and populations could not be grouped according to their pedigrees or collection area. Analysis of molecular variance (AMOVA) revealed that a larger part of the genetic variation exists among Greek walnut populations within a collection region (89%) than among the regions (11%). The pairwise regional PhiPT values indicated that the most geographically distant regions are the most genetically differentiated. The high variability existing in the Greek germplasm in combination with their valuable agro-morphological traits suggested that it would be beneficial to utilize this native germplasm pool in walnut breeding programs and germplasm management activities to maximize genetic diversity in cultivated walnut. 相似文献
15.
Floriana Fiocchetti Barbara Laddomada Mariaincoronata Roselli Paola Crinò Sergio Lucretti 《Scientia Horticulturae》2009
Italian lentil landraces are principally cultivated for self or local consumption. Most of them are disappearing, particularly macrosperma types by being less required by the market. A pre-requisite for the conservation and the efficient use of genetic resources is the better understanding of the extent and the distribution of the existing genetic variation, useful for future breeding programmes. Our study was undertaken to analyse and quantify the genetic diversity within and among three macrosperma Italian lentil landraces (Onano, Altamura and Villalba), using fluorescent AFLP markers. AFLP markers generated information to differentiate among closely related genotypes and group within the same cluster individuals belonging to the same landrace. The total genetic diversity (HT), the genetic diversity within population (HS) and the extent of differentiation between populations (DST) were 0.198, 0.155 and 0.043, respectively. The fixation index (GST = 0.219) showed that about 78% of the observed total genetic variation can be attributed to within population differences and around 22% is due to differences among populations. The gene flow estimate (Nm = 1.774) and the mean genetic distance value (0.077) suggested narrow genetic base among the analysed populations, confirming the tendency of Italian lentil landraces to group together. The present study showed that fluorescence-based AFLP technique is a biotechnological tool that can provide significant insights for research in genetic diversity of lentil landraces and their subsequent conservation and utilization in breeding programs. 相似文献
16.
Dendrobium loddigesii Rolfe is an endangered perennial herb with ornamental and medicinal value. Due to habitat deterioration and human over-exploitation, it has suffered a significant decline in abundance. Determining the level of genetic diversity and pattern of population genetic structure of this species would be helpful for its conservation and management. In this paper, sequence-related amplified polymorphism (SRAP) markers were applied to assess the level and pattern of genetic diversity in seven populations of D. loddigesii. Seventeen SRAP primer combinations generated a total of 231 clear amplification bands encompassing 187 (80.95%) polymorphic bands. A high level of genetic diversity was detected (PPB = 80.52%, H = 0.2743, I = 0.4113) at the species level. There was a moderate genetic differentiation (Gst = 0.304) among populations. Two main clusters were detected by cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA). Mantel test revealed that no significant positive correlation was found between genetic distances and geographic distances (r = 0.2302; P > 0.05). Recommendations for conservation of the endangered species resources are proposed. 相似文献
17.
新疆野苹果群体遗传结构和遗传多样性的SRAP分析 总被引:15,自引:2,他引:13
采用SRAP标记,对中国新疆野苹果4个种下居群的群体遗传结构和遗传多样性进行了研究。结果表明:10对SRAP引物总共扩增了209条带,其中206条是多态性带(98.56%)。巩留群体、新源群体、霍城群体和裕民群体分别扩增了180、169、178和165条多态性带,巩留群体的随机交配杂合度(hs = 0.3037 ± 0.0058)最高,其次为霍城群体。UPGMA聚类分析和群体间遗传分化系数显示,巩留群体和新源群体之间,以及霍城群体和裕民群体之间,遗传关系最近,群体间遗传变异最低。新疆野苹果群体内遗传变异高于群体间,占总变异的87.9%,主坐标轴分析显示4个群体是相对独立,其中巩留群体和新源群体,霍城群体和裕民群体之间,有较高的基因交流。所有参数分析表明,巩留群体遗传多样性最丰富,故在制定新疆野苹果原地和异地种质保护计划时应优先考虑巩留群体。 相似文献
18.
芍药野生居群父系分析与遗传结构研究 总被引:4,自引:0,他引:4
采用AFLP分子标记,对中国芍药(Paeonia lactiflora Pallas)代表居群——山西省交城县嵛岭山居群半同胞家系进行了父本组成、繁殖适合度、花粉散布、代际间遗传多样性水平和空间遗传结构的分析。9对引物共得到清晰判读的AFLP扩增带255条,其中多态性条带113条。对半同胞家系的父系分析结果表明,25个候选父系亲本在95%的可信度内检测出总的雄性繁殖贡献率为78.07%。芍药仅存在微弱的自交(自交率为2.25%),花粉散布以5 m为中心呈现尖峰分布,最远可达50 m,平均花粉散布距离23 m。亲代的多态性比率(P)为42.75%,平均Nei’s基因多样度指数(He)为0.1683 ± 0.2083,Shannon指数(I)为0.2462 ± 0.2982;子代P为52.94%,He为0.1921 ± 0.2055,I为0.2855 ± 0.2920。0 ~ 32 m距离范围内呈现显著的空间遗传结构。研究结果表明,芍药作为一种异交植物,花粉散布尺度小,受地理距离的影响显著,花粉散布符合邻近距离散布模式;遗传多样性水平不高,亲本和子代代际之间的遗传多样性水平差别不明显;近距离范围内存在显著的空间遗传结构。 相似文献
19.
基于SSR 标记的四川野生中国樱桃遗传多样性和居群遗传结构分析 总被引:9,自引:0,他引:9
采用SSR 分子标记技术对四川野生中国樱桃5 个居群共133 株的遗传多样性水平及居群的
遗传结构进行了研究。结果显示:10 对SSR 引物共检测到78 个等位基因,平均每位点等位基因7.8 个。
Nei’s 基因多样性指数(H)为0.6112 ~ 0.6689,Shannon’s 信息指数(I)为1.1984 ~ 1.3786。基于分子方
差分析(AMOVA),92.53%的变异来自居群内,7.47%的遗传变异来自于居群间。居群间遗传距离(GD <
0.2416)、遗传一致度(GI > 0.7854)、遗传分化指数(Fst = 0.0844)以及较强的基因流(Nm = 2.7125)均
表明居群间的遗传分化水平较低,居群内存在显著近交现象(Fis = 0.3986),且居群在大多数位点上偏离
Hardy-Weinberg 平衡。基于上述结果,分析讨论了居群较高遗传多样性和居群间较低遗传分化形成的可能
原因,并提出野生中国樱桃的保护利用策略。 相似文献
20.
Weiguo Zhao Yuhuang Wang Tingting Chen Gaofeng Jia Xiaoming Wang Jinliang Qi Yanjun Pang Sisi Wang Zhonghai Li Yongping Huang Yile Pan Yong-Hua Yang 《Scientia Horticulturae》2007
Mulberry is a perennial and economically important plant that has traditionally been used for feeding the silkworm. Evaluating genetic relationship is important for long-term improvement in mulberry yield, quality and resistance, and for germplasm conservation and identification. Population structure and genetic diversity of 8 mulberry populations from different ecotypes in China were analyzed by ISSR markers. Twelve ISSR primers generated a total of 83 amplification products, of which 50 were polymorphic, revealing 60.24% polymorphism among 66 mulberry local varieties, the mean PIC value was 0.1469. The total heterozygosity (HT), heterozygosity within population (HS), diversity between populations (DST) were 0.1600, 0.0851 and 0.0749, respectively. The coefficient of population differentiation (GST) was 0.4683, indicating that the variations among populations and those within populations contributed 46.8% and 53.2% to the total heterozygosity, respectively. The gene flow (Nm) was 0.5678, suggesting that genetic drift between populations can caused local genetic differentiation and therefore, population divergence. The mean genetic similarity coefficient was 0.8456, genetic similarity coefficient among 8 mulberry populations ranged from 0.8441 to 0.9640, indicating that genetic diversity of different populations existed variation. A dendrogram of all 66 local varieties of mulberry based on the genetic similarity using ISSR markers was generated by UPGMA cluster method. In the dendrogram, most varieties from the same ecotype clustered together. 相似文献