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不同贮藏温度下兰州百合种球淀粉代谢与萌发关系初探 总被引：36，自引：5，他引：36
不同的贮藏温度和贮藏时间，兰州百合鳞茎碳水化合物含量均有显著差异。顶芽6℃处理的淀粉含量最高，10℃处理最低；鳞茎盘的淀粉含量与之相反；鳞片的淀粉含量随着贮藏温度的降低而下降。鳞茎各部位可溶性糖的含量以及顶芽和鳞片的淀粉酶活性随着贮藏温度的升高而降低，鳞茎盘的淀粉酶活性为6℃处理最高，2℃处理最低。淀粉含量和淀粉酶活性呈极显著负相关。贮藏初期的34 d，顶芽在鳞茎内迅速发育，鳞茎内物质变化最活跃：淀粉含量明显下降，淀粉酶活性迅速增大，可溶性糖含量显著升高。顶芽和鳞茎盘的变化幅度大于鳞片，外部鳞片的变化幅度大于中部和内部鳞片。贮藏温度与贮藏时间之间的显著互作影响碳水化合物的代谢及鳞茎的萌发。在本试验中，2℃贮藏101 d为解除休眠的最佳处理。 相似文献
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GA3、IBA以及变温处理对东方百合鳞片扦插繁殖及淀粉降解的影响 总被引：2，自引：1，他引：1
以东方百合‘Sorbonne’为试材,研究了GA3、IBA以及温度处理对鳞片扦插繁殖以及碳水化合物代谢的影响。结果表明：GA3处理可在较短时间内促进小鳞茎膨大,IBA处理使小鳞茎形成的时间延后,但可提高繁殖系数和整齐度。GA3、IBA分别结合5 ℃预处理可使小鳞茎质量显著增加,在较短时间内促进小鳞茎膨大;其中IBA 100 mg · L-1,1 h结合5 ℃预处理的鳞片繁殖系数和小鳞茎整齐度依次高于25 ℃与15 ℃变温培养及25 ℃恒温培养;GA3 100 mg · L-1,2 h结合5 ℃预处理获得的小鳞茎品质最佳。鳞片繁殖过程中,GA3、IBA结合各温度处理鳞片淀粉及总可溶性糖含量均呈下降趋势,尤其鳞片下部在25 ℃培养初期（20 d）下降幅度最大;IBA促进淀粉降解,结合低温处理的母鳞片较无低温处理的总可溶性糖含量低,为小鳞茎的诱导和形成提供了营养;5 ℃低温预处理促进GA3处理母鳞片淀粉的降解。 相似文献
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以番红花鳞茎为试材,进行不同变温处理,采用蒽酮比色法、3,5-二硝基水杨酸比色法和高速氨基酸自动分析仪,测定不同时间段花芽内可溶性糖、淀粉、淀粉酶和游离氨基酸含量,探讨低温冷藏与番红花发芽休眠解除之间的关系。结果表明:处理30 d后,可溶性糖含量急剧上升,淀粉含量急剧下降,α-淀粉酶和β-淀粉酶活性均表现出与可溶性糖含量相似的变化趋势;5℃/10℃处理30 d后花芽中可溶性蛋白质含量显著上升,精氨酸和脯氨酸含量显著下降;3个处理的谷氨酸含量均呈现整体上升趋势;多项测定指标之间呈显著或极显著相关。由此可知,番红花花芽经30 d低温处理后开始解除休眠,且以5℃/10℃处理效果最佳。 相似文献
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AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
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多效唑对猕猴桃离体试管苗生长及内源激素的影响 总被引：18，自引：0，他引：18
多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。 相似文献
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HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献
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Duncan Brean W. Boyle Shannon Breininger David R. Schmalzer Paul A. 《Landscape Ecology》1999,14(3):291-309
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere. 相似文献
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AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis. 相似文献
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Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations
from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations,
dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture
or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near
Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between
November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation
was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme
temperatures (summer maximums, winter minimums). The correlation (r
2) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation
in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences
respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds
that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for
37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest
species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain
the patchy distribution of many species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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AIM: To detect the expression of cytoplasmic inhibitor of apoptosis protein 2 (c-IAP2) and growth arrest-specific gene 1 (GAS1) in Hodgkin's lymphoma (HL) and anaplastic large cell lymphoma (ALCL) and to investigate the role of two genes in the pathogenesis of HL and ALCL.METHODS: HE staining, the antibodies CD30, CD15, CD45RO and CD20 were used to screen the cases of HL and ALCL from 288 cases of lymphoma. The clarified HL and ALCL were subjected for immunohistochemical staining by SP and ABC methods to analyze the expression of c-IAP2 and GAS1. RESULTS: ①The positive rate of c-IAP2 in HL was 25/26(96.1%) while that in ALCL was 6/19(31.6%), there presented statistic significance between HL and ALCL groups(P<0.05), meanwhile the positive rate of GAS1 showed statistic significance between HL and ALCL groups(P<0.05). ②Two cases were showed to be a mixed type combined with large tumor cells of HL and relatively smaller tumor cells of ALCL.CONCLUSION: ①The different expression of c-IAP2 and GAS1 in HL and ALCL implied a different mechanism of oncogenesis and the different defects in the pathway of signal transduction of apoptosis in HL and ALCL;②Few cases showed an overlap and a likely transitional state between HL and ALCL; ③The different expressing manner of GAS1 and c-IAP2 in HL and ALCL implied the potential marks for the differential dignosis of two kinds of lymphoma. 相似文献
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Effects of patch attributes, barriers, and distance between patches on the distribution of a rock-dwelling rodent (Lagidium viscacia) 总被引：1，自引：0，他引：1
We tested whether size of habitat patches and distance between patches are sufficient to predict the distribution of the mountain
vizcacha Lagidium viscacia a large, rock-dwelling rodent of the Patagonian steppe Argentina, or whether information on other patch and landscape characteristics
also is required. A logistic regression model including the distance between rock crevices and depth of crevices, distance
between a patch and the nearest occupied patch, and whether or not there was a river separating it from the nearest occupied
patch was a better predictor of patch occupancy by mountain vizcachas than was a model based only on patch size and distance
between patches. Our results indicate that a simple metapopulation analysis based on size of habitat patches and distance
between patches may not provide an accurate representation of regional population dynamics if patches vary in habitat quality
independently of patch size and features in the matrix alter connectivity.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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β3-adrenergic receptor is the third subtype of β-adrenergic receptors. The genetic structure and pharmacological property of β3-adrenergic receptor are markedly distinguished from β1-and β2-adrenergic receptor subtypes. Recently studies show that myocardial β3-adrenergic receptor mediates negative inotropic effect through Gi-protein/NO/cGMP pathway, the expression of β3-adrenergic receptor and negative inotropic effect mediated by β3-adrenergic receptor are increased in heart failure. However, because of the low expression of β3-adrenergic receptor in the heart, the actual pathophysiological significance of β3-adrenergic receptor remains unknown. 相似文献