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Serological relationships between five fungally transmitted cereal viruses and other elongated viruses 总被引：2，自引：0，他引：2
F(ab')2 and protein A ELISA tests were used to investigate the serological affinities of five fungally transmitted cereal viruses: barley yellow mosaic (BaYMV), barley mild mosaic (BaMMV), oat mosaic (OMV), wheat yellow mosaic (WYMV) and oat golden stripe (OGSV). Within this group only BaYMV and WYMV were related. Chinese and UK isolates of BaYMV appeared to be similar. In tests using antisera to 29 other elongated viruses, BaYMV was related to one isolate of bean yellow mosaic poty virus (BYMV-G) and OGSV had affinities with BYMV-G, potato virus M, red clover vein mosaic (both carlaviruses) and perhaps Hordeum mosaic virus. The results were confirmed in immunoelectron microscopic tests. No affinities were found for BaMMV, OMV or WYMV. 相似文献
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Djabbar Hariri Michel Meyer Hayat Prud'homme 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(9):921-928
In March 2002 in a French field, severe mosaic symptoms appeared on plants of the barley cultivar Tokyo with the rym5 locus controlling resistance to all European strains of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV). Electron microscopic examination revealed that the disease symptoms were associated with the presence of flexuous particles which resemble bymoviruses. From these observations and after enzyme-linked immunosorbent assay analysis it was first determined that the plants could be infected by BaMMV and BaYMV. Mechanical transmission of these viruses to the barley cultivar Magie susceptible to both viruses was only possible for BaMMV. This new pathotype (BaMMV-Sil) from Sillery (Marne Department, 51, France), in contrast to another mechanically transmitted French BaMMV isolate (BaMMV-MF), could be transmitted mechanically to two barley cultivars (Tokyo, Misato Golden), Arachis hypogaea, Datura stramonium and Lactuca sativa. BaMMV-Sil was indistinguishable from three BaMMV isolates from Germany (G), Japan (Ka1) and France (PF) by monoclonal antibodies in ELISA while the Japanese isolate (Na1) and BaMMV-MF were distinguishable from all. The sequence of the 3-terminal region of BaMMV-Sil RNA1 was determined. Comparison with previously published sequence data of capsid proteins indicated that BaMMV-Sil was closely related to BaMMV-Ka1, BaMMV-G and another German isolate (BaMMV-ASL1). Resistance-breaking BaMMV strains able to infect cultivars carrying the rym5 locus have also been described in Japan (BaMMV-Na1) and Korea (BaMMV-Kor). No specific amino acid differences were detected between the capsid proteins of BaMMV-Sil, BaMMV-Na1, BaMMV-Kor and those BaMMV isolates that do not overcome the rym5 resistance gene. These results indicate that BaMMV-Sil is a new pathotype of BaMMV in France and suggests that the capsid protein is not the determining factor of the pathogenicity towards the resistance gene rym5. 相似文献
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Molecular comparisons amongst wheat bymovirus isolates from Asia, North America and Europe 总被引：2，自引：0，他引：2
Jiong Chen Sohn Chen Lei Cheng Schulze Steinbiss Antoniw & Adams 《Plant pathology》1999,48(5):642-647
To study the variation between wheat bymovirus isolates and to resolve uncertainties about the identity of the virus in some countries, leaves of infected plants were obtained from nine sites in China and from one each in Italy, Germany, USA and Canada. The German isolate was obtained from rye and the Canadian isolate was the type strain of wheat spindle streak mosaic virus (WSSMV). In RT-PCR, using primers designed from a partial sequence of a French isolate (tentatively described as WSSMV), genome fragments were obtained from the Italian and the French isolates but not from the Chinese ones. Conversely, products were consistently obtained from the Chinese isolates, but not from the Italian or French ones, when primers were designed from the sequence of a Japanese isolate of wheat yellow mosaic virus (WYMV). Nucleotide sequences were obtained from regions at or near the 3'-terminus of RNA1 of six Chinese isolates and the four from Europe and North America, usually including the coat protein. Nucleotide and amino acid sequence comparisons demonstrated that the European and North American isolates were extremely similar and were therefore WSSMV, while the Chinese isolates were close to the Japanese isolate and were thus WYMV. 相似文献
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核苷酸序列分析结果表明,小麦黄色花叶病毒(W YMV)不同分离物的外壳蛋白基因存在一定的差异。邓州分离物CP基因在其31~33nt处均缺失了3个核苷酸,其余分离物与潢川分离物及日本分离物长度一致,均为882nt。不同分离物CP基因核苷酸序列同源性为97.3%~98.9%,由此推导的氨基酸序列同源性为97.6%~99.3%,外壳蛋白N末端的110个氨基酸和C末端的55个氨基酸在各个分离物间是高度保守的。潢川分离物有5个氨基酸与其它5个分离物明显不同。WYMV不同分离物外壳蛋白序列分析结果进一步确认了WYMV与WSSMV为Bymovirus属的2种不同病毒。 相似文献
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用香石竹斑驳病毒(CarMV)免疫的BALB/c鼠脾细胞与Sp2/0鼠骨髓瘤细胞融合,经筛选克隆,获得5株能稳定传代且分泌抗CarMV单克隆抗体(MAb)的杂交瘤细胞,并分别制备它们的单克隆抗体腹水。5株单克隆抗体腹水间接ELISA效价达10-6,其中3G1、1B9、2A9和2F8的抗体类型及亚类均为IgG1,而2F2为IgG3。Western-blot分析表明,5株单克隆抗体均与CarMV 38 kD的外壳蛋白亚基有特异性反应。利用2A9单抗建立的抗原包被的间接ELISA (ACP-ELISA)检测CarMV方法,病叶1:800倍稀释、提纯CarMV病毒浓度为1 ng/mL (绝对检测量为0.1 ng)时仍能检测到病毒。利用ACP-ELISA对田间香石竹样品的检测表明,CarMV在香石竹上发病很普遍。 相似文献
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Djabbar Hariri Michel Meyer 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(1):1-10
In April 2001, stunted barley plants bearing mosaic symptoms were observed in a field in France (Marne Department, 51). Rod-shaped
and flexuous particles were visualized by electron microscopy and positive serological reactions were detected by ELISA with
Barley yellow mosaic virus (BaYMV) and Soil-borne cereal mosaic virus (SBCMV) polyclonal antisera. The tubular virus which was soil transmissible to barley cv. Esterel was separated from BaYMV
by serial mechanical inoculations to barley cv. Esterel. This furo-like virus, in contrast to a French isolate of SBCMV, could
be transmitted to Hordeum vulgare, Avena sativa, Beta vulgaris and Datura stramonium. RT-PCR was used to amplify the 3′-terminal 1500 nucleotides of RNA1 and the almost complete sequence of RNA2. Nucleotide
and amino acid sequence analyses revealed that the French virus infecting barley is closely related to a Japanese isolate
of Soil-borne wheat mosaic virus (SBWMV-JT) which was originally isolated from barley. This French isolate was named SBWMV-Mar. The 3′ UTRs of both RNAs can
be folded into tRNA-like structures which are preceded by a predicted upstream pseudoknot domain with seven and four pseudoknots
for RNA1 and RNA2, respectively. The four pseudoknots strongly conserved in RNAs 1 and 2 of SBWMV-Mar show strong similarities
to those described earlier in SBWMV RNA2 and were also found in the 3′ UTR of Oat golden stripe virus RNAs 1 and 2 and Chinese wheat mosaic virus RNA2. Sequence analyses revealed that the RNAs 2 of SBWMV-Mar and -JT are likely
to be the product of a recombination event between the 3′ UTRs of the RNAs 2 of SBWMV and SBCMV. This is the first report
of the occurrence of an isolate closely related to SBWMV-JT outside of Japan. 相似文献
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小麦黄花叶病毒(WYMV)2个山东分离物的全基因组序列及进化分析 总被引：1，自引：1，他引：0
利用5'RACE结合一步法RT-PCR分别从山东泰安与临沂冬小麦上克隆了小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV)的全基因组序列。这2个分离物(TADWK和LYJN)基因组间核苷酸一致性分别为97.21%(RNA1)和95.12%(RNA2)。通过对目前已报道的共14个分离物的基因组不同部分的分析,表明5'UTR是WYMV基因组变化幅度最大的区域,而编码区(ORF)及3'UTR的序列一致性较高且变动幅度小。另外,发现LYJN RNA2的5'UTR与已报道的所有分离物RNA2的核苷酸一致率仅为90%左右。综合分析RNA1和RNA2的系统发生树,表明WYMV各基因组片段呈单独进化特征,不同分离物间存在RNA重排。RNA重组分析显示在LYJN RNA1和TADWK RNA2发现了RNA重组。此研究说明WYMV在山东地区存在分离物分化现象,WYMV的5'UTR是基因组中的突变热点。 相似文献
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Incidence of Soil-borne Wheat Mosaic Virus in Mixtures of Susceptible and Resistant Wheat Cultivars 总被引：1，自引：0，他引：1
Djabbar Hariri Marc Fouchard Hayat Prud'homme 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(6):625-631
The multiplication of Soil-borne wheat mosaic virus (SBWMV) was studied in mixtures of two winter wheat (Triticum aestivum) cultivars, one susceptible (Soissons) and the other resistant (Trémie). Two seed mixtures of susceptible and resistant varieties in ratios of 1 : 1 and 1 : 3 and their component pure stands, i.e. each variety grown separately, were grown in a field infected with SBWMV. The presence of the virus was detected using DAS-ELISA from January to May. The resistant cultivar Trémie showed no foliar symptoms nor could the virus be detected in the leaves or roots. In May, about 88% of plants of susceptible cultivar Soissons grown in pure stands were infected. At this time, the disease reduction relative to pure stands was 32.2% in the 1 : 1 mixture and 39.8% in the 1 : 3 mixture. Optical density (OD) values from ELISA of the infected plants in the two mixtures were consistently lower than that of the infected plants in cultivar Soissons in pure stands. The ELISA index (EI) calculated using three scales of OD values was 65.5% in the susceptible cultivar in pure stands. The value for this index was 19.1% in the 1 : 1 mixture and 7.9% in the 1 : 3 mixture. The plants of the resistant cultivar Trémie infected in the same field and transferred in January to a growth cabinet at 15 °C multiplied the virus and produced viruliferous zoospores. These results show that the resistant cultivar Trémie plays a role in disease reduction in the cultivar mixtures in field conditions. Possible reasons for this are discussed. 相似文献
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小麦黄花叶病(WYNW)的研究 总被引：3，自引：0，他引：3
小麦黄花叶病的病状是黄色花叶。病株细胞内有1至数个内含体。病毒质粒为线状,稍弯曲,大小为13×100-600毫微米,存在于细胞质中。内含体呈风轮状。病毒仅感染小麦属(Triticum)10多种,能通过病根、病土及它们侵出液传染。介体为禾谷多粘霉(Polymyxa graminis),其寄主有小麦等28种禾本科植物。病土加灭菌土1000倍混合仍有致病性,病田表土致病性最强,深到31-40厘米处还会发病。田间3月中旬以后病株不再增加。予防本病以选育选育抗病品种为主,辅以轮作,增施肥料和迟播等。 相似文献
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Differences in cultivar response and complete sequence analysis of two isolates of wheat yellow mosaic bymovirus in China 总被引：1，自引：0，他引：1
J. Chen J.-P. Chen † J.-P. Yang Y. Cheng A. Diao M. J. Adams J. Du 《Plant pathology》2000,49(3):370-374
Seeds of selected European and Japanese winter wheat cultivars were grown at two experimental sites in China, namely Yaan, Sichuan province (YA), and Yangzhou, Jiangsu province (YZ), where wheat yellow mosaic bymovirus (WYMV) was severe. There were some differential responses of the cultivars to the virus isolates present at the two sites. The complete nucleotide sequence of both RNAs of both virus isolates was determined. Their genome organization was identical to that reported for a Japanese isolate and the sizes were very similar. Nucleotide comparisons demonstrated that parts of the CI and NIa coding regions on RNA1 and the N-terminal part of the P2 coding region on RNA2 were particularly variable, while substantially conserved regions occurred in the 3' UTR of RNA2, the 7K, one part of the CI and parts of the NIb and coat protein. It seems unlikely that differences in the 7K and NIa-VPg proteins are responsible for virulence differences and the CI and NIb regions were considered the most promising for further study. 相似文献
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为建立南方菜豆花叶病毒(southern bean mosaic virus,SBMV)的快速、简便、高通量检测技术,加强该病毒的口岸检验检疫,以提纯的SBMV粒子为免疫原免疫BALB/C小鼠,利用杂交瘤技术获得3株杂交瘤细胞株19C3、19H9和20G4,其分泌的SBMV腹水单抗效价均达到10-7,且3个单抗与感染SBMV大豆叶片组织粗提液有强烈的特异性免疫反应,而不与感染南方豇豆花叶病毒(southern cowpea mosaic virus,SCPMV)的豇豆、健康的大豆、毛豆、豌豆、蚕豆和菜豆叶片组织粗提液发生免疫反应。以制备的单抗为核心,建立了检测植物中SBMV的ACP-ELISA和dotELISA两种血清学方法。3个单抗中19H9单抗的检测灵敏度最高,以其建立的ACP-ELISA和dot-ELISA方法检测大豆病叶粗提液的灵敏度分别达到1∶163 840和1∶10 240稀释浓度。利用建立的dot-ELISA方法可从上海口岸截获的大豆种子中检测出SBMV,且该检测结果得到RT-PCR方法验证。表明制备的SBMV单抗及建立的SBMV血清学检测技术可有效用于我国SBMV的口岸检验检疫。 相似文献
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中国小麦黄花叶病毒(WYMV)分布的RT-PCR鉴定 总被引：16，自引：3，他引：16
镰刀菌产生的单端孢霉烯毒素,包括DON、DAS和T-2毒素,是一类重要的植物病原真菌毒素。这类毒素不仅加强病菌的为害程度,而且人畜食用罹病谷物及其制成品易造成中毒。近年来,单端孢霉烯毒素的生物合成途径、关键酶及其基因克隆,毒素在致病过程中的作用,以及利用毒素作为选择压鉴定小麦抗病性和筛选抗病突变体均取得了明显进展。本文对此作一综述。 相似文献
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小麦黄花叶病是由禾谷多黏菌传播的小麦黄花叶病毒引起的病害,近年在黄淮麦区呈蔓延加重趋势。为了给病害的防治和抗病育种工作提供依据,本研究利用分级评价方法对黄淮地区推广的小麦品种进行了田间抗病性鉴定。两年鉴定结果表明,在145个供试小麦品种中,‘濮优938’、‘新麦208’、‘豫麦416’、‘新原958’、‘豫麦70-36’、‘泛麦5号’等70个品种表现为免疫,占总数的48.28%;‘豫麦47’和‘邯6172’表现为抗病,占总数的1.38%;‘洪育2号’、‘花培2号’、‘偃展4110’、‘豫麦41’、‘郑麦9023’等48个品种表现为中抗,占总数的33.10%;‘兰天06129’、‘兰天0591’、‘徐麦9158’、‘徐麦0054’、‘徐麦1108’等25个品种表现为感病,占总数的17.24%。研究结果为指导小麦黄花叶病区合理选择小麦品种提供了科学依据。 相似文献
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D. Hariri M. Meyer J. Le Gouis N. Bahrman M. Fouchard C. Forget A. Andre 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(4):365-372
The reaction of thirty-four barley cultivars from European and Asiatic origin was analysed in six soils infected with barley yellow mosaic virus complex (BaYMV, BaMMV). These soils were selected from 16 sites for their differences in cultivar response. Amongst the six cultivars carrying the ym4 gene (Esterel, Express, Labéa, Majestic, Réjane, Vanoise), only cv Majestic was infected at one site with BaYMV and BaMMV. Concerning BaYMV, three cultivars were infected on all the soils and 19 on none of them. Twelve cultivars were differentially infected depending on the soil. In the case of BaMMV, four cultivars were infected on all the soils and 19 on none of them. Eleven cultivars were differentially infected depending on the soil. ELISA tests revealed the presence, in these soils, of variants of BaYMV and BaMMV that were able to overcome at least seven of the 12 known resistance genes (ym3, ym4, ym6, ym8, ym9, ym10, ym11) and the resistance of three varieties (Tosan Kawa 73, OU1 and Taihoku A) in which the genetic basis is unknown. Amplification by RT-PCR of the N-terminal region for three of BaYMV variants was performed. Nucleotide and amino acid sequences were determined and compared with the corresponding sequence of a common strain of BaYMV-G. A few nucleotide differences were detected between all the French isolates, but there were no strain specific amino acid differences. 相似文献
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小麦对黄花叶病的抗性鉴定及典型品种的遗传分析 总被引：2，自引：0，他引：2
本研究针对来自江苏、河南、四川、湖北、日本和美国的37个小麦品种,在进行小麦抗黄花叶病的抗病性鉴定、调查的基础上,结合分子生物学检测(RT-PCR和ELISA),证实小麦品种扬辐9311对小麦黄花叶病毒(WYMV)表现免疫。将该品种与表现高感的小麦品种进行杂交和回交,通过对其后代抗感分离的调查与分析,确定了小麦品种扬辐9311对黄花叶病的抗性受一对显性基因控制,并为寻找小麦抗黄花叶病基因的分子标记提供理论依据和实验材料。同时,本文对小麦抗黄花叶病表现的影响因素及宁麦9号的抗病性进行了分析和讨论。 相似文献
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M. J. ADAMS 《Plant pathology》1991,40(1):53-58
Improved diagnosis of barley yellow mosaic (BaYMV) and barley mild mosaic (BaMMV) viruses was obtained by adjusting the buffers used in immunospecific electron microscopy (ISEM) to ensure a pH ≥ 7-0 and in ELISA by replacing ovalbumin with 10 g/l full cream milk powder.
Over 70% of samples of winter barley with symptoms of mosaic received from different sites in the UK during 1987-90 had BaYMV and 37% had BaMMV, with 11% containing both viruses. BaMMV was much more common on malting cultivars than on those grown for feed and this resulted in an easterly bias to the geographical distribution of the virus. Both viruses were, however, widely distributed in areas where winter barley is grown intensively. A small number of BaYMV records were from cultivars previously regarded as resistant and these are probably a distinct strain of the virus. 相似文献
Over 70% of samples of winter barley with symptoms of mosaic received from different sites in the UK during 1987-90 had BaYMV and 37% had BaMMV, with 11% containing both viruses. BaMMV was much more common on malting cultivars than on those grown for feed and this resulted in an easterly bias to the geographical distribution of the virus. Both viruses were, however, widely distributed in areas where winter barley is grown intensively. A small number of BaYMV records were from cultivars previously regarded as resistant and these are probably a distinct strain of the virus. 相似文献