共查询到19条相似文献，搜索用时 593 毫秒
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为了研究金钗石斛复方对小鼠免疫功能的影响,将实验小鼠随机分为对照组、高剂量组、中剂量组、低剂量组;采用Con A诱导小鼠淋巴细胞转化试验及迟发性变态反应试验测试试验组小鼠的细胞免疫功能;采用抗体生成细胞检测及溶血试验测试其体液免疫功能;采用小鼠腹腔巨噬细胞吞噬鸡红细胞试验测试其特异性免疫功能.结果表明,与对照组比较,金钗石斛复方能够明显提高脾淋巴细胞增殖能力、溶血空斑数、小鼠抗体积数、巨噬细胞吞噬鸡红细胞的能力;金钗石斛复方对提高小鼠NK细胞活性能力无显著影响.金钗石斛复方对正常小鼠的细胞免疫、体液免疫及巨噬细胞功能均有明显的增强作用. 相似文献
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为了探讨抑制脂多糖结合蛋白(LBP)基因表达对水牛单核巨噬细胞在内毒素(LPS)诱导下炎症相关基因的表达,首先采用三质粒慢病毒包装系统包装LBP基因shRNA重组质粒pSicoR-GFP-shLBP774.利用病毒颗粒感染水牛单核巨噬细胞,进行荧光定量qRT-PCR及ELISA检测.结果显示,单核巨噬细胞在用5×108 IU/mL滴度的LBP-shRNA774慢病毒颗粒,感染指数(MOI)为300、感染7d的条件下,感染效率超过50%.qRT-PCR检测结果显示,与LPS对照组相比,shLBP774感染组可极显著抑制LBP基因的表达(p0.01),CD14,TLR4,TNF-α,IL-1β,IL-8等基因表达显著降低(p0.05).ELISA检测结果发现,与对照组相比,细胞分泌的TNF-α,IL-1β蛋白量显著降低(p0.05).以上结果表明,抑制LBP基因表达可以有效降低LPS诱导下单核巨噬细胞炎症相关基因的表达,进而调控LPS引发的炎症反应,提示可将LBP作为靶基因深入研究水牛单核巨噬细胞免疫功能和LPS致炎信号传导分子机制. 相似文献
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研究绞股蓝黄芪保健茶对小鼠免疫功能的影响。将200只清洁级ICR雌性小鼠,按体重分成免疫5大组,每组随机分成4小组,每小组10只;按人日推荐用量的5倍、10倍、30倍,分别设0.415、0.830、2.490g/kg-Bw 3个剂量组和蒸馏水对照组。小鼠每天按20mL/kg BW连续灌胃30d后,分别测定小鼠的体重、脏器指数、细胞免疫功能、体液免疫功能、单核-巨噬功能以及NK细胞活性。结果表明:样品高剂量组小鼠淋转OD差值、抗体积数、溶血空斑数和碳廓清吞噬指数a都明显高于对照组,经统计,差异均有统计学意义(p0.05);高剂量的样品对小鼠的免疫功能有明显的增强作用。 相似文献
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[目的]研究复方霍山石斛浸膏对小鼠免疫力的影响。[方法]将200只健康雌性小鼠分为5个试验组,每组分高、中、低和空白对照4个剂量组[3.0、1.0、0.5、0 g/(kg.bw)],连续灌胃给药30 d后分别对不同剂量组小鼠进行细胞免疫的功能检测(ConA诱导小鼠脾淋巴细胞转化试验、DNFB诱导小鼠DTH试验)、体液免疫功能检测(抗体生成细胞检测试验、血清溶血素测定试验)、单核-巨噬细胞功能检测(小鼠炭粒廓清试验、小鼠腹腔巨噬细胞吞噬鸡红细胞试验)和NK细胞活性测定。[结果]各项检测结果与空白对照组相比,均能刺激小鼠脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数和血清溶血素水平,增强小鼠的单核-腹腔巨噬细胞吞噬、碳廓清能力及NK细胞的活性。[结论]复方霍山石斛浸膏具有增强免疫力功能。 相似文献
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评价乳铁蛋白牛初乳颗粒增强免疫力作用,为该产品提供功能学资料。采用SPF级雌性小鼠,设0.200,0.04,1.200 g/kg(分别相当于人体推荐量的5,10,30倍)3个剂量组,每日1次经口给予小鼠相应浓度的受试样品,连续给样1个月。检测对小鼠体重、胸腺、脾脏器官、ConA诱导的脾淋巴细胞转化、DNFB诱导的DTH、溶血空斑数、血清半数溶血值(HC50)、碳廓清能力、腹腔巨噬细胞吞噬鸡红细胞、NK细胞活性的影响。结果表明:细胞免疫功能、体液免疫功能、NK细胞活性均与对照组有显著差异,认为该乳铁蛋白牛初乳颗粒具有增强免疫力功能效果(P<0.05)。 相似文献
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研究沙棘果粉、库拉索芦荟提取物、三七提取物制成的复配物对小鼠免疫力的影响。实验设0.625 g/kg.bw、1.250 g/kg.bw、3.750 g/kg.bw三个剂量组和阴性对照组,经口灌胃30天后,考察样品对小鼠免疫脏器/体重比值、免疫细胞功能、体液免疫功能、单核-巨噬细胞功能和NK 细胞活性等的影响。结果表明,与对照组比较,中剂量和高剂量能显著提高小鼠迟发型变态反应能力、NK细胞活性、抗体生成细胞数(P<0.05),高剂量能显著提高小鼠半数溶血值(P<0.05),说明复配物能够增强小鼠的免疫力。本研究为相关产品开发提供了功能依据。 相似文献
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【目的】研究党参总皂苷纳米乳(total saponins of Codonopsis pilosula nanoemulsion,TSCP-NE)对小鼠免疫功能的影响,为TSCP-NE作为免疫增强剂用于保健品或药品提供理论依据。【方法】将小鼠分为空白对照组、模型对照组、空白纳米乳组、TSCP水溶液组、TSCP-NE高中低剂量组,空白对照组和模型对照组小鼠灌胃蒸馏水,空白纳米乳组小鼠灌胃空白纳米乳,TSCP水溶液组灌胃200 mg/kg TSCP,TSCP-NE高中低剂量组分别灌胃200,100,50 mg/kg TSCP-NE,连续灌胃14 d(1次/d),用药量0.01 mL/g。除空白对照组外,其余各组小鼠在给药第1天皮下注射氢化可的松(HY),连续注射7 d,制备免疫抑制小鼠,检测分析免疫抑制小鼠细胞免疫(血清IL-2和IFN-γ含量、ConA诱导脾细胞增殖活性)、体液免疫(血清溶血素水平)和非特异性免疫(NK细胞杀伤活性、单核巨噬细胞吞噬活性)相关指标。【结果】TSCP-NE为透明淡黄色O/W纳米乳,平均粒径为60.67 nm,粒径多分散值为0.347,折光率、pH值、粘度、浊点分别是1.340 6,6.79,4.38×10~(-3)(Pa·s),60.5℃。50～200 mg/kg TSCP-NE能显著提高免疫抑制小鼠血清IL-2和IFN-γ水平、脾T细胞增殖刺激指数、NK细胞杀伤率、血清溶血素半数溶血值(HC_(50))、单核巨噬细胞廓清指数和吞噬指数;TSCP-NE免疫增强作用具有量效依赖性,随着给药剂量增加,免疫增强作用明显增大,且其作用显著强于同剂量TSCP水溶液。【结论】TSCP-NE能增强TSCP对细胞免疫、体液免疫和非特异性免疫的调节功能,可以作为免疫增强剂用于保健品或药品。 相似文献
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通过4种中药复方对小鼠的脾脏指数、体重、腹腔巨噬细胞吞噬指数和吞噬率、溶血素、溶血空斑、淋巴细胞增殖、血清中一氧化氮(NO)、一氧化氮合酶(NOS)、自然杀伤细胞(NK细胞)的影响作用,测得不同中药复方与对照组的差异。结果表明,复方1对小鼠的脾脏指数、腹腔巨噬细胞吞噬率和吞噬指数、血液中溶血素水平差异显著,复方2对小鼠的脾脏指数、腹腔巨噬细胞吞噬率、血液中溶血素和溶血空斑差异显著;复方3对小鼠吞噬指数、血液中溶血素和溶血空斑、淋巴细胞增殖(ConA)差异显著;复方4对小鼠的脾脏指数、腹腔巨噬细胞吞噬率和吞噬指数、血液中溶血素和溶血空斑、淋巴细胞增殖(LPS)、血液中NO和NK细胞均差异显著。不同中药复方中复方4的免疫增强作用最强。 相似文献
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Brown MG Dokun AO Heusel JW Smith HR Beckman DL Blattenberger EA Dubbelde CE Stone LR Scalzo AA Yokoyama WM 《Science (New York, N.Y.)》2001,292(5518):934-937
Natural killer (NK) cells are lymphocytes that can be distinguished from T and B cells through their involvement in innate immunity and their lack of rearranged antigen receptors. Although NK cells and their receptors were initially characterized in terms of tumor killing in vitro, we have determined that the NK cell activation receptor, Ly-49H, is critically involved in resistance to murine cytomegalovirus in vivo. Ly-49H requires an immunoreceptor tyrosine-based activation motif (ITAM)-containing transmembrane molecule for expression and signal transduction. Thus, NK cells use receptors functionally resembling ITAM-coupled T and B cell antigen receptors to provide vital innate host defense. 相似文献
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NKR-P1, a signal transduction molecule on natural killer cells 总被引：23，自引：0，他引：23
R Giorda W A Rudert C Vavassori W H Chambers J C Hiserodt M Trucco 《Science (New York, N.Y.)》1990,249(4974):1298-1300
Natural killer (NK) cells are a subpopulation of large granular lymphocytes characterized by densely staining azurophilic granules. NK cells are able to recognize and lyse various virally infected or neoplastic target cells without previous sensitization or major histocompatibility complex restriction. A 60-kD disulfide-linked dimer, highly expressed on NK cells, was found capable of mediating transmembrane signaling. The gene encoding this signal transduction molecule was cloned and its nucleotide sequence determined. The encoded protein showed significant homology with a number of lectin-related membrane proteins that share receptor characteristics. This protein may function as a receptor able to selectively trigger NK cell activity. 相似文献
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类风湿关节炎是慢性自体免疫性疾病,文章从作用于细胞因子及其受体、针对T淋巴细胞的清除、影响G蛋白偶联受体信号转导和Ras-丝裂原激活的蛋白激酶信号转导四个方面阐述了消炎免疫药物治疗类风湿性关节炎的分子作用机制。 相似文献
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【目的】构建表达牛源犬新孢子虫NcSAG1基因的重组腺病毒株,并接种Balb/c小鼠,评价重组腺病毒株对Balb/c小鼠的体液免疫和细胞免疫应答水平。【方法】PCR扩增牛源犬新孢子虫NcSAG1基因,构建pMD18-T-NcSAG1克隆质粒和pCR259-NcSAG1重组腺病毒穿梭质粒;将鉴定正确的pCR259-NcSAG1重组腺病毒穿梭质粒依次转化HighQ-1 Transpose-Ad 294和HighQ-1™感受态细胞,构建Transpose-Ad-NcSAG1重组腺病毒表达质粒;PacI酶切线性化后,脂质体介导转染QBI-HEK 293细胞包装重组腺病毒Ad5-NcSAG1,应用PCR和Western blotting技术检测Ad5-NcSAG1及其表达蛋白产物。测定Ad5-NcSAG1重组腺病毒滴度后,接种Balb/c小鼠,测定小鼠血清中IgG特异性抗体和细胞因子IFN-γ、IL-4水平,以此评价重组腺病毒株的免疫应答效果。【结果】扩增的牛源犬新孢子虫NcSAG1基因大小为982bp,与GenBank中发表的NcSAG1（AF132217）核苷酸序列的同源性为99.2%;经PCR和Western blotting检测,重组腺病毒Ad5-NcSAG1在QBI-HEK 293细胞中包装成功,表达蛋白的分子量为33kD,具有较好的反应原性;测得重组腺病毒Ad5-NcSAG1滴度为1010TCID50/mL,Ad5-NcSAG1接种Balb/c小鼠后,能够诱导产生高水平的IgG特异性抗体和IFN-γ、IL-4细胞因子。说明Ad5-NcSAG1重组腺病毒对Balb/c小鼠产生了较强的体液免疫和细胞免疫应答。【结论】成功构建了牛源犬新孢子虫NcSAG1基因的重组腺病毒株,该毒株能够诱导Balb/c小鼠产生高水平的体液免疫和细胞免疫应答,为犬新孢子虫新型疫苗的临床试验奠定了基础。 相似文献
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Heil F Hemmi H Hochrein H Ampenberger F Kirschning C Akira S Lipford G Wagner H Bauer S 《Science (New York, N.Y.)》2004,303(5663):1526-1529
Double-stranded ribonucleic acid (dsRNA) serves as a danger signal associated with viral infection and leads to stimulation of innate immune cells. In contrast, the immunostimulatory potential of single-stranded RNA (ssRNA) is poorly understood and innate immune receptors for ssRNA are unknown. We report that guanosine (G)- and uridine (U)-rich ssRNA oligonucleotides derived from human immunodeficiency virus-1 (HIV-1) stimulate dendritic cells (DC) and macrophages to secrete interferon-alpha and proinflammatory, as well as regulatory, cytokines. By using Toll-like receptor (TLR)-deficient mice and genetic complementation, we show that murine TLR7 and human TLR8 mediate species-specific recognition of GU-rich ssRNA. These data suggest that ssRNA represents a physiological ligand for TLR7 and TLR8. 相似文献
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Fogg DK Sibon C Miled C Jung S Aucouturier P Littman DR Cumano A Geissmann F 《Science (New York, N.Y.)》2006,311(5757):83-87
Macrophages and dendritic cells (DCs) are crucial for immune and inflammatory responses and belong to a network of cells that has been termed the mononuclear phagocyte system (MPS). However, the origin and lineage of these cells remain poorly understood. Here, we describe the isolation and clonal analysis of a mouse bone marrow progenitor that is specific for monocytes, several macrophage subsets, and resident spleen DCs in vivo. It was also possible to recapitulate this differentiation in vitro by using treatment with the cytokines macrophage colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. Thus, macrophages and DCs appear to renew from a common progenitor, providing a cellular and molecular basis for the concept of the MPS. 相似文献
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多糖免疫机制研究进展 总被引：14，自引：0，他引：14
综述了近年来多糖对动物免疫功能影响的研究进展,包括多糖受体的研究、多糖对免疫细胞基因表达的影响、多糖对淋巴细胞信息传导的影响、多糖对巨噬细胞一氧化氮的影响和多糖对前列腺素的影响等。 相似文献
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Bone marrow-derived lymphoid cells (B cells): functional depletion with cobra factor and fresh serum 总被引：2，自引：0，他引：2
Treatment of rat spleen cells with cobra factor and fresh rat serum provided a simple, rapid means of functionally eliminating complement receptor lymphocytes. Cells able to differentiate into plaque-forming cells in a syngeneic, irradiated host were diminished, but cells able to induce a graft-versus-host reaction were not diminished. There was no effect on plaque-forming cells from an immune spleen. 相似文献
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The experiment was conducted to study the dynamic changes of immune responses of chicks immunized with Marck‘s disease(MD) trivalent vaccine and turkey herpesvirus (HVT)at one day age.Results were found that after immunization of chicks with MD vaccines,the interlcukine-2 (IL-2) inductive activity and IL-2 receptor expression of T cells from thymus and spleen significantly increased.suggesting that the immunoregulative function was markedly enhanced in the immune organs;the number of antibody-producing cells,the number and proliferative function of T cells rose markedly in Bursa Fabricius,Splcen and thymus,indicating that the ccllular and humoral immune responses were elevated remarkablly in the central and peripheral immune organs; the number of T and antibody-producing cells as well as the content of IgG,IgA and IgM obviously mounted in cecal tonsil,Harder ian gland mucosal lymphoid tissucs of bronchus along with tears,trachea washings,bilc and intestinal fluids,demonstrating that the local and mucosal immunity was raised in the respiratory and digestive tract;the levels of immune responses mentioned above in the trivalent vaccine-immuniacd chicks were apparently higher than those of HVT-immunized birds. 相似文献