共查询到19条相似文献，搜索用时 234 毫秒
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化学诱导型启动子能够从时空上精确调控基因的表达,是研究基因产物的有力工具。利用植物内源性化学诱导启动子或其他生物的化学诱导响应元件都可以在植物中构建化学诱导表达系统。文章介绍了化学诱导表达系统的类型及其构建原理,并对其在植物基因工程中的应用提出了展望。 相似文献
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细菌通过信号转导网络系统感知外界的环境变化，并通过调节自身基因表达，控制菌体的行为、代谢以及发育等。细菌和古细菌大多数使用双组分转导系统和化学传感系统感知环境变化。基于基因组研究发现在细菌的双组份转导系统和化学传感系统中存在多种不同的胞外结构域，这些胞外结构域能感知胞外信号，对细菌的各种生理功能具有重要作用。介绍了Cache结构域、CHASE结构域、NIT结构域以及4HB结构域这4类胞外感觉结构域的结构特性以及功能，为胞外感觉结构域的分类以及识别信号的位点提供借鉴。 相似文献
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微生物表达系统是用于蛋白质生产及性质研究的重要基础。常用的微生物表达系统有大肠杆菌表达系统,酵母表达系统和丝状真菌表达系统。该文主要介绍了上述表达系统的优缺点,并作比较分析。 相似文献
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由于DNA重组技术和蛋白组技术的发展与成熟,外源基因表达技术备受关注。其在生物、食品、工业以及医学领域发挥着举足轻重的作用。以原核生物细胞及真核生物细胞作为表达系统,进行目的基因序列的表达以生产蛋白疫苗、核酸疫苗和生物酶制剂等是近年来发酵工业的新型领域。原核和真核表达系统是近年来研究和应用最广泛和最成熟的外源基因表达系统。对近年来关于大肠杆菌表达系统和酵母表达系统的研究进展进行了综述。 相似文献
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大肠杆菌表达系统的研究进展 总被引：3，自引：0，他引：3
随着蛋白组时代的到来,近年来对蛋白质结构以及功能的研究进一步深入,重组蛋白技术的应用范围也越来越广泛。大肠杆菌表达系统以其成本低廉、生产率高、操作简单等优点备受青睐,常常作为表达重组蛋白的首选表达系统。但是,如何选择合适的表达系统来高效的表达目的蛋白还需要考虑多方面的因素,包括表达载体、表达宿主菌、表达条件等。综述了常用的大肠杆菌表达系统的组成以及影响外源基因表达的相关因素。 相似文献
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论述了ACC合成酶的分子生物学研究进展,阐述了系统1乙烯和系统2乙烯形成过程中ACC合成酶基因的表达特点. 相似文献
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ZHANG Shu-juan LI Yu-lian SONG Guo-qi GAO Jie ZHANG Rong-zhi LI Wei CHEN Ming-li LI Gen-ying 《农业科学学报》2020,19(4):941-952
Th IPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat(Triticum aestivum L.) production. The present study investigated whether the application of the Th IPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized Th IPK2 gene was transferred into common wheat through Agrobacterium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29 A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of Th IPK2 in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type(WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content(RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized Th IPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering. 相似文献
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Regulation of the Amount of Starch in Plant Tissues by ADP Glucose Pyrophosphorylase 总被引：2，自引：0，他引：2
Starch, a major storage metabolite in plants, positively affects the agricultural yield of a number of crops. Its biosynthetic reactions use adenosine diphosphate glucose (ADPGlc) as a substrate; ADPGlc pyrophosphorylase, the enzyme involved in ADPGlc formation, is regulated by allosteric effectors. Evidence that this plastidial enzyme catalyzes a rate-limiting reaction in starch biosynthesis was derived by expression in plants of a gene that encodes a regulatory variant of this enzyme. Allosteric regulation was demonstrated to be the major physiological mechanism that controls starch biosynthesis. Thus, plant and bacterial systems for starch and glycogen biosynthesis are similar and distinct from yeast and mammalian systems, wherein glycogen synthase has been demonstrated to be the rate-limiting regulatory step. 相似文献
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《农业科学学报》2017,(9)
Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants(e.g., soybean), which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies. 相似文献
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植物基因工程为果树育种开辟了一条新途径,在果树重要性状遗传改良方面具有重要意义。果树转基因是一个复杂而长期的工作,包括从对外源基因功能的全面认识、高效表达载体的构建、基因转化、以及转基因植株的鉴定与性状观测等多个重要环节。应该说,每一个环节甚至每一实验步骤都需严格把握。迄今,鉴于已经有大量果树再生体系成功建立的报道,笔者在总结实际研究经验的基础上,结合文献的报道,有针对性地对果树基因转化技术和转基因植株的鉴定与性状分析进行简要介绍,为果树基因转化技术的选用,以及进行高效转基因植株的鉴定与性状分析提供一定的参考。 相似文献
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风速预测对风电场和含风电的电力系统的运行都具有重要意义。对风速进行比较准确的预测,可以有效地减轻或避免风电场对电力系统的不利影响,同时提高风电场在电力市场中的竞争能力,因此预测方法得到广泛地发展。本文重点分析随机时间序列法、工神经网络法、卡尔曼滤波法、模糊逻辑法、小波分析法、空间相关法等几种基本方法的原理。 相似文献