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1.
Summary Different nitrogen sources (NO3 −, NH4 +, glutamic acid and their combinations) influenced the growth and morphogenic responses (node number, shoot length, and stem, leaf and root dry weight) of three micropropagated potato cultivars (Spunta, Kennebec, Huinkul). Addition of reduced nitrogen (NH4 + or glutamic acid) in a nitrate medium increased shoot length and leaf number. The large increase in growth in plants fed with NO3 −, NH4 + could be explained by higher organic nitrogen content and enhanced dry matter partition to the shoot. This suggests that reduced nitrogen source is required, at least as a supplement to NO3 −, to enhance N assimilation and growth.  相似文献   

2.
The effect of controlled irrigation and drainage on N leaching losses from paddy fields was investigated by controlling root zone soil water content and water table depth using a lysimeter equipped with an automatic water table control system. Three treatments that combined irrigation and drainage managements were implemented: controlled irrigation (CI) + controlled water table depth 1 (CWT1), CI + controlled water table depth 2 (CWT2), and flooding irrigation (FI) + actual field water table depth (FWT). Controlled irrigation and drainage had significant environmental effects on the reduction of NH4 +–N and NO3 ?–N leaching losses from paddy fields by decreasing water leakage. The NH4 +–N leaching losses from CI + CWT1 and CI + CWT2 were 3.68 and 4.45 kg ha?1, respectively, which significantly reduced by 59.2 and 50.7 % compared with FI + FWT (9.02 kg ha?1). The NO3 ?–N leaching losses from CI + CWT1 and CI + CWT2 were 0.88 and 0.43 kg ha?1 with a significant reduction of 45.2 and 73.2 %, respectively, compared with FI + FWT (1.61 kg ha?1). The application of CI + CWT1 can be a pollution-controlled water management method of reducing N leaching losses from paddy fields.  相似文献   

3.
We test the hypothesis that reduction in grain N concentration under elevated CO2 concentration (e[CO2]) is associated with N types (NH4+ and NO3−) and their ratios. Wheat (Triticum aestivum L. cv. H45) was grown in a glasshouse under two CO2 concentrations (389 μmol mol−1 and 700 μmol mol−1), supplied with equal amount of N with different ratios of NH4+ and NO3−: (i) 100% NO3−–N; (ii) 50% NO3−–N and 50% NH4+–N; and (iii) 25% NO3−–N and 75% NH4+–N. Plant growth, N uptake and partitioning were measured during plant development. Plant biomass and grain yield was increased at e[CO2] when N was supplied as an equal proportion of NO3− and NH4+. Despite the yield increment, grain N concentration was not affected by e[CO2], in 50% NO3−–N treatment. In contrast, grain N concentration decreased in 100% NO3−–N and 25% NO3−–N treatments. In 50% NO3−–N treatment, N uptake during post-anthesis stage (from 69 to 141 days after planting) was significantly stimulated under e[CO2] compared to 100% NO3−–N and 25% NO3−–N treatments. We concluded that supplement of N in an equal proportion of NO3− and NH4+ which increases post-anthesis N uptake, avoid the reduction of grain N concentration under e[CO2].  相似文献   

4.
The present study was carried out to evaluate nutrient losses that occur during the course of agricultural activity from rice paddy fields of reclaimed tidal flat. For this study, we chose a salt-affected rice paddy field located in the Saemangeum reclaimed tidal area, which is located on the western South Korean coasts. The plot size was 1,000 m2 (40 m × 25 m) with three replicates. The soil belonged to the Gwanghwal series, i.e., it was of the coarse silty, mixed, mesic type of Typic Haplaquents (saline alluvial soil). The input quantities of nitrogen and phosphorus (as chemical fertilizer) into the experimental rice paddy field were 200 kg N ha−1 and 51 kg P2O5 ha−1 per annum, and the respective input quantities of each due to precipitation were 9.3–12.9 kg N ha−1 and 0.4–0.7 kg P ha−1 per annum. In terms of irrigation water, these input quantities were 4.5–8.2 kg N ha−1 and 0.3–0.9 kg P ha−1 per annum, respectively. Losses of these nutrients due to surface runoff were 22.5–38.1 kg N ha−1 and 0.7–2.2 kg P ha−1 for the year 2003, and 26.8–29.6 kg N ha−1 and 1.6–1.9 kg P ha−1 for the year 2004, respectively. Losses of these nutrients due to subsurface infiltration during the irrigation period were 0.44–0.67 kg N ha−1 and 0.03–0.04 kg P ha−1 for the year 2003, and 0.15–0.16 kg N ha−1 and 0.05–0.06 kg P ha−1 for 2004. When losses of nitrogen and phosphorus were compared to the amount of nutrients supplied by chemical fertilizers, it was found that 11.3–19.1% of nitrogen and 0.5–1.7% of phosphorus were lost via surface runoff, whereas subsurface losses accounted to 0.2–0.8% for nitrogen and only 0.02–0.04% for phosphorus during the 2-year study period.  相似文献   

5.
Intensive use of chemical fertilizer for crops may be responsible for nitrogen and phosphate accumulation in both groundwater and surface waters. The return flow polluted by nutrients not only results in the limitation of water reuse goals but also creates many environmental problems, including algal blooms and eutrophication in neighboring water bodies, posing potential hazards to human health. This study is to evaluate the N-fertilizer application of terraced paddy fields impacting return flow water quality. Water quality monitoring continued for two crop-periods around subject to different water bodies, including the irrigation water, drainage water at the outlet of experimental terraced paddy field, and shallow groundwater were conducted in an experimental paddy field located at Hsin-chu County, Northern Taiwan. The analyzed results indicate that obviously increasing of ammonium-N (NH4 +-N) and nitrate-N (NO3 ?-N) concentrations in the surface drainage water and ground water just occurred during the stage of basal fertilizer application, and then reduced to relatively low concentrations (<0.1 mg/l and <3 mg/l, respectively) in the remaining period of cultivation. The experimental results demonstrate the potential pollution load of nitrogen can be reduced by proper drainage water control and fertilizer application practices.  相似文献   

6.
Nitrogen fertilization is one of the factors that influences Bemisia tabaci (Gennadius) population density. The aim of this study was to determine the effects of three N application rates (75, 205 and 335 mg/l) and three ratios of NO3−:NH4+ ions (92:8, 75:25 and 55:45) in standard nutrient solution (205 mg/l N) on the population density of B. tabaci. The experiments were conducted on spring-summer hydroponic crops of tomato. The effect of plant stratum on the whitefly population was also determined. The aggregation of B. tabaci adults as well as their oviposition rate was higher at 205 and 335 mg/l N than on plants grown at 75 mg/l N. By the end of the experiment (60 d after infestation), the number of nymphs on plants at 205 mg/l N was higher than on plants at 75 mg/l N. The number of pupae was lowest on plants supplied with 75 mg/l N. An increase in NH4+ percentage in standard nutrient solution (from 25% to 45% of the total N) reduced adult population density and oviposition rate. The density of nymphs and pupae, at 60 d after infestation, was lower on the tomato plants grown at 75:25 and 55:45 NO3−:NH4+ ratios compared to the 92:8 ratio. The 75:25 and 55:45 NO3−:NH4+ ratios resulted in a higher incidence of blossom-end rot of tomato fruit, with a lower incidence of disorder at 75:25 than at the 55:45 ratio. Plant stratum influenced adult whitefly distribution in two years of the study. Middle stratum leaves were more attractive to adults in both years. The results demonstrate the effects of N fertilization (N rate and the ratio of NO3−:NH4+) and plant stratum on B. tabaci population density.  相似文献   

7.
We investigated the groundwater flow and the transport and potential source of groundwater nitrates in the typical karst setting of the Ryukyu Limestone aquifer in the southern part of Okinawa Island, Japan. Analysis of groundwater hydrographs indicated that this is a ??mixed flow?? aquifer with the coexistence of slow diffuse flow in the matrices and rapid conduit flow in the caves and caverns. This relationship is indicated by the travel time of groundwater flow: 70?days in the matrices of the aquifer and 6?days through the caves and caverns. The conduit flow system was also confirmed by the distribution of relatively low concentrations of 222Rn near caverns. The sampling sites were categorized into upland field (UF) type and residential area (RA) type according to the land-use ratio on the upstream side with a 600-m influential radius, and cave and cavern (CC) type according to the hydrogeologic setting near two large caverns, even though the CC type should be categorized as the UF type from the viewpoint of land use. Cross plots of NO3-N versus SO4 2? showed that the predominant source of UF groundwater nitrates was chemical fertilizer. A difference was observed in average ??15N values between UF (8.9??) and RA (10.0??). On the other hand, the average ??15N value for CC (10.5??) was similar to that for RA, indicating that CC nitrates were not related to the surrounding land use. This phenomenon is considered as evidence that CC groundwater nitrates were carried by rapid groundwater flow through caves and caverns from residential areas located higher upstream compared to the influential areas. According to previous studies, animal and human waste was considered the predominant sources of RA and CC groundwater nitrogen. The contribution ratio of chemical fertilizer (R CF) was calculated using mass balance equations under assumed predictability. There was a relatively high correlation between the rate of upland areas and of residential areas and R CF. Average R CF for UF, RA and CC was 41, 27, and 25%, respectively.  相似文献   

8.
A water and nitrogen balance model for the surface ponded water compartment of rice fields was developed. The model estimates the daily ponded water depth and the daily losses and the uses of NH4–N and NO3–N in their transformation processes. The model was applied with data obtained from two rice fields during 2005 at Thessaloniki plain in northern Greece. Significant amounts of applied irrigation water were lost with the surface runoff and deep percolation to groundwater. The gaseous losses of nitrogen (volatilization and denitrification) and nitrogen uptake by algae were the main processes of nitrogen reduction in the ponded water of rice fields. The study showed that the system of a rice field is a natural system where an important amount of influent nitrogen applied by irrigation water can be reduced. These processes decrease the possibilities of water resources contamination.  相似文献   

9.
Nitrous oxide (N2O) emission from flooded rice paddy fields was continuously measured by the closed chamber method at an experimental plot in Thailand for a whole cultivation period. To characterize the N2O emission with regard to the denitrification N loss, the C2H2 inhibition method was applied. Flood water on the soil greatly suppressed the N2O emission. The N2O emission was mitigated considerably by even a thin film of the flood water. The overall average N2O emissions under flood conditions for one crop season (83 days) at the control site and the C2H2 treated site were 10.3 and 11.8 μg N m−2 h−1, respectively. The N2O emission from the C2H2 treated site was consistently higher than that from the control site and the N2O emission from both sites followed the same diurnal and seasonal variation pattern, indicating the effect of denitrification inhibition by the supplied C2H2. The N2O emission enhanced along with temperature increase when NO3–N concentration in the soil water was above 0.4 mg N l−1 and soil temperature was above 24°C, suggesting specific temperature influence over the emission. The increase in NO3–N concentration and temperature in the soil affected only the N2O emission while the difference in the emission at the C2H2 treated site and the control site was not so much affected. It was suggested that most of the actively produced N2O under higher NO3–N concentration and temperature would likely to quickly emit to the atmosphere rather than to undergo further reduction to N2.  相似文献   

10.
An investigation of the carbon economy of single S184 white clover plants nodulated with an effective strain of Rhizobium trifolii growing on N-free nutrient solution and supplied with 150 parts/106 N as NH4NO3 has shown that 10% more of the C fixed per day is available for growth in the plants supplied with combined N. The difference between the two groups of plants is a result of higher respiratory activity in the roots of plants growing exclusively on N2. In terms of shoot growth, however, the difference in growth rate is likely to be less than 10%, because the rate of root growth is greater in the plants supplied with a moderate level of combined N. There is no evidence that NO-3 and CO2 compete for photosynthetically produced reductant in the leaves of plants grown on N2+NH4NO3, since no reduction in net photosynthesis was observed in plants assimilating combined N. An experiment carried out on detached leaflets of white clover grown on N2 and on N2+ NH4NO3 has shown that NO-3 -reducing activity is present in the leaflets of plants grown on N2+ NH4NO3 but not in plants grown on N2 and that the activity is light-dependent. Measurements of 14CO2 respired in the light and dark by leaflets previously allowed to photosynthesize 14CO2 showed that the ratio of 14CO2 output in the light to 14CO2 output in the dark was no higher in plants grown on N2+ NH4NO3 than in plants grown on N2. This observation is consistent with the finding that N source does not affect net photosynthesis in whole plants.  相似文献   

11.
为了解不同氮效率小麦品种根系氮代谢特征及其吸收能力的差异,明确小麦氮高效利用的生理机制,在水培条件下,研究了氮高效小麦品种漯麦18和氮低效小麦品种西农509的根系氮代谢特征和对NO-3、NH+4吸收的动力学特征。结果表明,漯麦18的根系GS活性、硝酸还原酶活性、游离氨基酸含量、可溶性蛋白质含量均高于西农509;而西农509的根系硝态氮和铵态氮含量高于漯麦18;漯麦18根系对NO-3、NH+4吸收的最大吸收速率(Vmax)显著高于西农509;漯麦18根系对NO-3、NH+4的亲和力(以Km的倒数衡量)低于西农509。结果说明,氮高效型小麦品种根系对NO-3、NH+4的吸收能力和同化能力均显著高于氮低效型小麦品种;小麦根系对NO-3、NH+4的吸收和同化是相互促进的关系。  相似文献   

12.
To valuate the multifunctionality economically is effective to make it possible to realize the value for the nation and to compare functionalities among countries of the world. In this paper, the external economies of paddy fields and fallow paddy fields including wetlands as N removal function sites, and of upland fields and orchards as pollution sites are valuated by the newly proposed replacement cost method, by replacing them with construction costs of water quality improvement facilities. In addition, we discuss an agricultural land-use scenario in which cultivated land has no net negative economic effect on the water environment. The results showed that (1) paddy fields and fallow paddy fields including wetlands were respectively valued at 1.2×103 and 2.81×103 JPY m−2 on average as the N removal sites, (2) upland fields had 0.32×103 JPY m−2 on average of economic value, and suggested that paddy fields have an external economic value that compensates for the negative external economic value of upland fields 3.65 times their size.  相似文献   

13.
The widely adopted alternate wetting and drying (AWD) irrigation for rice production is increasingly needed to quantify the different water outflows and nitrogen leaching losses. We investigated the effects of AWD on percolation, water productivity, nitrogen leaching losses, and nitrogen productivity through in situ experiments. Results show that AWD reduced irrigation water without a significant impact on grain yields and increased the mean water productivity by 16.9 % compared with continuously flood irrigation (CFI). The mean nitrogen productivity of 135 kg ha?1 N level was 22.2 % higher than that of 180 kg ha?1 N level, although grain yields substantially increased because of nitrogen fertilization application. The percolation was also reduced by 15.3 % in 2007 and 8.3 % in 2008 compared to CFI. However, the cumulative percolation of the first 5 days after irrigation in AWD plots is significantly larger than that in CFI plots. The NH4 +–N and TN leaching losses of AWD and CFI had no significant variations while the NO3 ?–N leaching losses were increased caused by AWD. The total NH4 +–N, NO3 ?–N, and TN leaching losses of AWD in the first 3 days after irrigation were higher than that of contemporaneous CFI. The results indicate that the bypass or preferential flow and strengthened nitrification–denitrification nitrogen transformation processes because of alternate wetting and drying potentially decrease the water saving effectiveness and increase the NO3 ?–N loading to the groundwater.  相似文献   

14.
The pool of nitrate-N (NO3–-N) in the soil is more prone to losses than that of ammonium-N (NH4+-N) so any shift towards NO3–-N dominance in the soil pools, caused by management intensity, could have environmental implications. The change in the balance of soil NH4+-N and NO3–-N content with time was studied using grazed grass swards receiving different fertilizer N inputs. In addition, the effect of past management on net nitrification of 400 μg NH4+-N g–1 was investigated in a soil incubation study. Mineral N was determined at frequent intervals (at least every 2 weeks) throughout the year in the top 5 or 7·5 cm of a sandy clay-loam soil at the Agricultural Research Institute of Northern Ireland at Hillsborough, County Down, for a 7-year period (1989–90 to 1995–96). The treatments were a perennial ryegrass–white clover sward receiving no fertilizer N, together with perennial ryegrass swards receiving 100, 200, 300, 400 or 500 kg N ha–1 year–1 as calcium ammonium nitrate. The plots were continuously grazed by beef steers from April to October to maintain a constant sward height of 7 cm. There was little or no change in average soil NO3–-N and NH4+-N content from 1989–90 to 1995–96 on the grass–clover sward and plots receiving 100 and 200 kg N ha–1 year–1. However, with the plots receiving 300, 400 and 500 kg N ha–1 year–1 NO3–-N became progressively more dominant with time. The incubation study confirmed that this was due to an increase in net nitrification rate. There was evidence that rapid microbial assimilation of NO3–-N occurred during the soil incubations. Past management history can play an important role in determining soil NO3–-N content and hence potential losses of N to the environment.  相似文献   

15.
Gymnema sylvestre is an important medicinal plant which bears bioactive compound namely gymnemic acids. The present work deals with optimization of cell suspension culture system of G. sylvestre for the production of biomass and gymnemic acid and we investigated effects of macro elements (NH4NO3, KNO3, CaCl2, MgSO4 and KH2PO4 - 0.0, 0.5, 1.0, 1.5 and 2.0× strength) and nitrogen source [NH4+/NO3− ratio of: 0.00/18.80, 7.19/18.80, 14.38/18.80, 21.57/18.80, 28.75/18.80, 14.38/0.00, 14.38/9.40, 14.38/18.80, 14.38/28.20 and 14.38/37.60 (mM)] of Murashige and Skoog medium on accumulation of biomass and gymnemic acid content. The highest accumulation of biomass (165.00 g l−1 FW and 15.42 g l−1 DW) was recorded in the medium with 0.5× concentration of NH4NO3 and the highest production of gymnemic acid content was recorded in the medium with 2.0× KH2PO4 (11.32 mg g−1 DW). The NH4+/NO3− ratio also influenced cell growth and gymnemic acid production; both parameters were greater when the NO3− concentration was higher than that of NH4+. Maximum biomass growth (159.72 g l−1 of FW and 14.95 g l−1 of DW) was achieved at an NH4+/NO3− ratio of 7.19/18.80, and gymnemic acid production was also greatest at the same concentration of NH4+/NO3− ratio (11.35 mg g−1 DW).  相似文献   

16.
A field experiment was carried out to research the changes and spatial distributions of soil nutrients in saline–sodic soil for different number of cultivated years under drip irrigation. The distributions of available potassium (AK), available phosphorus (AP), nitrate nitrogen (NO3 ?–N), ammonium nitrogen (NH4 +–N), as well as the amount of total nitrogen (TN), total phosphorus (TP) and organic carbon (OC) in the 0–40 cm soil layers in saline–sodic soils planted with Leymus chinensis for 1, 2, and 3 years were studied. The results showed that the distance from the emitter had an obvious effect on soil nutrients. Drip irrigation had substantial effects on levels of AK, AP, and NO3 ?–N. The contents of AK, AP, and NO3 ?–N were very high in the area near the emitter in the horizontal direction. In the vertical direction, levels of all of the available and total soil nutrients decreased with increased soil depth. Levels of AK, AP, NO3 ?–N, NH4 +–N, TN, TP, and OC all increased with continued cultivation of crops on saline–sodic soil using drip irrigation. Compared to the nutrients found in soils from the natural L. chinensis grasslands, the contents of AK and TP were higher in the drip-irrigated soils, although the contents of AP, NO3 ?–N, and NH4 +–N were broadly comparable. Given the rate of improvements in nutrient levels, we forecast that the nutrients in drip-irrigated saline–sodic soils should match those of the natural L. chinensis grasslands after 3–6 years of cultivation.  相似文献   

17.
《Plant Production Science》2013,16(2):138-143
Abstract

Potato tuber initiation and its growth are key processes determining tuber yield, which are closely related to stolon growth, and are influenced by many factors including N nutrition. We investigated the influences of different forms of nitrogen (N) on stolon and tuber growth in sand culture with a nitrification inhibitor during 2010 – 2011, and using two potato cultivars. Plants supplied with NO3-N (N as nitrate, NO3-) produced more and thicker stolons than those supplied with NH4-N (N as ammonium, NH4+) at tuber initiation stage. In the plants fed NO3-N, the stolon tips swelled or formed tubers earlier and produced more tubers than in those fed with NH4-N. However, no significant difference was observed among N forms in terms of tuber yield at harvest, this may have been because of the shoot growth rate at tuber initiation stage was lower in the plants fed NO3-N. During the tuber bulking stage, the difference in shoot DWs among N forms began to decrease, and the shoot DW of plants fed NO3-N was even heavier than those fed NH4-N in some cases. The influence of N form on potato plant growth may therefore vary with the potato growth stage.  相似文献   

18.
Flooded paddy fields have many functions, including not only rice production, and ecological and environmental conservation. This work estimates the extent of paddy field infiltration in Taiwan by adopting a one-dimensional Darcy-based soil/water balance model SAWAH (Simulation Algorithm for Water Flow in Aquatic Habitats). A 10 cm thick plow sole layer with a hydraulic conductivity of 0.03 cm/day, coupled with the soil texture and irrigation data obtained from 15 irrigation associations, is used to estimate the volumetric amount of annual infiltration in Taiwan. Simulation results from SAWAH indicate that the plow sole layer controls the movement of infiltrated water, with a rate about 1.8 billion cubic meters annually. The estimated infiltration rate of 1.8 billion m3/yr comprises more than 40% of the annual infiltration recharge to ground water in Taiwan. Additionally, the amount of infiltration recharge to groundwater is equivalent to 20 billion Taiwan dollars NT$ (or 0.65 billion US$) while the yearly rice crop production is 35 billion NT$ (or 1.13 billion US$). It is evident that the infiltration from rice paddy is of great importance to the economy, environment, and water resources conservation in Taiwan.  相似文献   

19.
为明确外源硝态氮对高铵胁迫下小麦幼苗生长的影响及其生化机理,采用温室水培的方式,以豫麦49(耐高铵品种)和鲁麦15(高铵敏感型品种)为材料,研究了外源硝态氮对高铵胁迫下小麦幼苗形态、激素含量和抗氧化系统的影响。结果表明,高铵胁迫条件下,外源硝态氮显著增加两个小麦品种株高、根长、干重,其中鲁麦15的地上部干重增加量高于豫麦49,而根系干重增加量则表现为豫麦49高于鲁麦15。高铵胁迫下,两个小麦品种植株的IAA、CTK含量、IAA/CTK显著低于对照;外源硝态氮处理5 d后,豫麦49地上部和根系IAA含量、根系CTK含量显著增加,恢复至对照水平;鲁麦15植株虽亦表现显著增加,但仍低于对照。另外,外源硝态氮对高铵胁迫下两个小麦品种地上部和根系的O■释放速率、SOD和POD活性及MDA含量没有显著影响。综上,外源硝态氮缓解小麦幼苗生长高铵胁迫的原因可能是通过增加IAA和CTK合成和转运,影响IAA和CTK之间的平衡,进而达到缓解效果。品种间比较,耐铵型品种豫麦49缓解作用可能源于对地上部和根系IAA含量以及根系CTK含量的协同调控;而高铵敏感型品种鲁麦15的缓解作用可能主要源于对地上部IAA含量的调控。  相似文献   

20.
The fertilization management of the rice crop in Piedmont was analyzed at a regional scale, and the agronomic and environmental sustainability of the actual fertilization strategy of rice was evaluated through the analysis of its effect on the soils and waters quality. On average, a total amount of 127 kg ha−1 of N, 67 kg ha−1 of P2O5 and 161 kg ha−1 of K2O were supplied to the rice crop. In most cases N and P fertilization was rather well balanced with crop removal. The N balance was in the range ±50 kg for 77% of the surface. The low concentration of N in the groundwater reflected the small N surplus. P fertilization resulted to be smaller than removal for 53% of the surface. Nevertheless, the soil extractable P was very high, probably because of former higher P inputs. This resulted in a high concentration in water courses and aquifers. The K fertilization was excessive (surplus >100 kg ha−1) for 53% of the surface, but most soils showed a low K content. K is probably contributing to nutrient leaching to a great extent. The average soil organic matter (SOM) content of paddy fields was higher than that of normally-cultivated soils in Piedmont, and the C/N was higher, owing to the low mineralization rate in waterlogged conditions. The SOM content was in relation with the management of the crop residues, as the tradition of burning straw after harvest was still widespread on 65% of the paddy surface.  相似文献   

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